WO2009071102A1 - Procédé et dispositif optique pour mesurer des concentrations de substances dans des fluides biologiques - Google Patents
Procédé et dispositif optique pour mesurer des concentrations de substances dans des fluides biologiques Download PDFInfo
- Publication number
- WO2009071102A1 WO2009071102A1 PCT/EE2008/000026 EE2008000026W WO2009071102A1 WO 2009071102 A1 WO2009071102 A1 WO 2009071102A1 EE 2008000026 W EE2008000026 W EE 2008000026W WO 2009071102 A1 WO2009071102 A1 WO 2009071102A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- concentration
- sample
- cuvette
- biological fluid
- substance
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 66
- 239000000126 substance Substances 0.000 title claims abstract description 34
- 239000013060 biological fluid Substances 0.000 title claims abstract description 28
- 230000003287 optical effect Effects 0.000 title claims description 24
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 claims abstract description 44
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 claims abstract description 44
- 229940116269 uric acid Drugs 0.000 claims abstract description 44
- 238000001228 spectrum Methods 0.000 claims abstract description 18
- 238000004422 calculation algorithm Methods 0.000 claims abstract description 14
- 238000005259 measurement Methods 0.000 claims abstract description 14
- 230000001131 transforming effect Effects 0.000 claims abstract description 8
- 238000000338 in vitro Methods 0.000 claims abstract description 6
- 238000012545 processing Methods 0.000 claims description 10
- 238000012544 monitoring process Methods 0.000 claims description 8
- 238000009499 grossing Methods 0.000 claims description 6
- 238000013079 data visualisation Methods 0.000 claims description 2
- 239000012530 fluid Substances 0.000 abstract description 2
- 238000000502 dialysis Methods 0.000 description 24
- 239000007788 liquid Substances 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 230000006870 function Effects 0.000 description 5
- 201000005569 Gout Diseases 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 4
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 3
- 201000001431 Hyperuricemia Diseases 0.000 description 3
- 238000004737 colorimetric analysis Methods 0.000 description 3
- 210000003734 kidney Anatomy 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 230000005855 radiation Effects 0.000 description 3
- 230000009897 systematic effect Effects 0.000 description 3
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 229960005070 ascorbic acid Drugs 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000001684 chronic effect Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000005518 electrochemistry Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000001631 haemodialysis Methods 0.000 description 2
- 230000000322 hemodialysis Effects 0.000 description 2
- 230000007257 malfunction Effects 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000004611 spectroscopical analysis Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000002211 L-ascorbic acid Substances 0.000 description 1
- 235000000069 L-ascorbic acid Nutrition 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108010092464 Urate Oxidase Proteins 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 108010046334 Urease Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000002615 hemofiltration Methods 0.000 description 1
- 208000007475 hemolytic anemia Diseases 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000000711 polarimetry Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000012088 reference solution Substances 0.000 description 1
- 238000000611 regression analysis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 210000003371 toe Anatomy 0.000 description 1
- ZNOKGRXACCSDPY-UHFFFAOYSA-N tungsten trioxide Chemical compound O=[W](=O)=O ZNOKGRXACCSDPY-UHFFFAOYSA-N 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
Definitions
- This invention relates to a novel method and a device for measuring concentration of substances, preferable uric acid, in biological fluids. More specifically, the present invention relates to an optical method utilizing spectrum of the biological fluid and the Savitzky-Golay algorithm to determine the concentration of the substances on-line.
- Uric acid a final product of the metabolism of purine, is very important biological molecule present in body fluids. It is mostly excreted from human body through the kidneys in the form of urine. The concentration of uric acid in blood increases when the source of uric acid increases or the kidney malfunctions. Hyperuricemia is a symptom when the uric acid concentration is above 7 mg/dL. Uric acid is hard to dissolve in blood and will crystallize when supersaturated. The uric acid crystallites deposit on the surface of skin, in joints, and especially in toes and results in gout. The analysis of the uric acid concentration in blood helps to diagnose gout.
- hyperuricemia In addition to gout, hyperuricemia is connected with lymph disturbance, chronic hemolytic anemia, an increase of nucleic acid metabolism and kidney malfunction. High caloric foods and alcohol as well as disturbances of organs and tissues are the main causes of hyperuricemia and even gout. Harm can be prevented and reduced by an early diagnosis and monitoring. A simple and inexpensive detecting system helps patients to detect the uric acid concentration on their own.
- Uric acid can be determined in several different ways. Two of the most common methods are (1) reduction method by the reaction of uric acid with alkaline phosphotungstate, and (2) the enzymatic method by the enzyme urease.
- reduction method by the reaction of uric acid with alkaline phosphotungstate
- enzymatic method by the enzyme urease.
- uric acid is estimated by reducing alkaline phosphotungstate to tungsten blue and measuring the colored product in a colorimeter.
- the demerits of this method include: 1) some compounds similar to uric acid and ascorbic acid contained in the sample of biological fluid affect the test accuracy; 2) the operation is complex, needs lots of agents which are hard to keep, and should be operated by professionals; 3) the sample must be de- protein pretreated; and 4) the necessary equipment is expensive.
- the second method detects uric acid by optical colorimetry and electrochemistry and is classified into uricase-ultraviolet absorption, uricase-peroxidase, uricase-catalase and uricase- electrode methods, wherein the former three methods make ⁇ se of the color of reaction products and quantitatively detect uric acid of by colorimetry.
- the decrease in absorbance is proportional to the amount of uric acid initially present.
- the automatic bio-analyzers used in central bio-laboratories of hospitals detect uric acid by optical colorimetry.
- An improving system for quantification of biochemical components in biological fluids during analysis where a component reacts with an analyte is described in US6121050.
- the blood sample should be pretreated to be serum or plasma first.
- the merits of the automatic bio-analyzers reside in mass detecting, automation and quickness.
- an automatic bio-analyzer cannot be applied in household detecting because it requires professionals to operate, is expensive, and is particularly hard to store the detecting agents.
- the uricase-electrode method detects uric acid by electrochemistry.
- the electrodes can be divided as enzymatic and non-enzymatic.
- the former produced by a complex production process is hard to store and thus is only suitable for research.
- the merits of the described method are that it does not need blood samples, no disposables or chemicals, and is fast.
- the described method is general and does not specify methodology to measure exclusively a single compound and is meant to apply only for dialysis monitoring. Moreover, no results about the concentration measurements are presented. More exact description about the uric acid and urea measurements using the abovementioned method is given in a scientific papers (Uhlin, Lindberg et al. 2005), (Uhlin, Fridolin et al. 2005).
- Another method relates to a method for dialysis monitoring method and apparatus using near infrared radiation, described in WO9819592.
- the merits of the described method are similar to that of the UV -radiation.
- the described method does not measure uric acid and utilizes near infrared radiation spectrometry with different technical and optical considerations.
- near infrared radiation spectrometry the principial component analysis using calibration and prediction stage is described in US5886347.
- the objective of the invention is, therefore, a new method and a device for measuring concentration of substances, such as uric acid, in biological fluids. More specifically, the present invention relates to an optical method utilizing optical spectrum of the biological fluid and the Savitzky-Golay (or Savitsky-Golay) algorithm, and concentration calculation algorithm containing the transforming function to determine on-line the concentration of the substances, which can be effected directly at the bed-side and which avoids the disadvantages caused by the analysis in a laboratory.
- Savitzky-Golay or Savitsky-Golay
- Another object of the present invention is to provide a novel and practical optical uric acid detecting method and device which detects concentration of uric acid in the biological fluids and can be represented directly and easily on the monitor or screen printed.
- the novel method and device does not require any chemical disposables, neither expensive uricase nor both L-ascorbic acid oxidase, and can be easily made and mass-produced providing an environment-friendly optical method.
- a still further object of the present invention is to provide a method for assessing routine clinical monitoring in order to face risks of higher mortality in patients (e.g. in dialysis).
- a still further object of the present invention is to provide a novel, rapid, convenient and safe method for detecting concentration of substances in a liquid sample.
- the liquid sample can be directly dropped on the detecting cuvette for in-vitro measurements or sent a flowing stream of fluid through a flow-cuvette for on-line monitoring.
- the method is suitable for household use when being applied to detect the concentration of substances in the biological fluids.
- Fig. 1 depicts a block diagram of one embodiment of the invention.
- Fig. 2 depicts a block diagram of another embodiment of the invention applied for uric acid concentration measurements during dialysis.
- Fig. 3 shows the linear relationship between uric acid (UA) concentration measured by a known method and at the laboratory.
- Fig. 4 shows the linear relationship between uric acid (UA) concentration measured by the invented (new) method and at the laboratory.
- the device for measuring concentration of certain substances (e.g., uric acid) 5 in a biological fluid 1 comprises: an optical module 2, comprising a spectrophotometrical system, comprising a light source and a light detector; a measuring cuvette for holding a sample of the biological fluid so that the light can be led through the sample; a signal processing module 3 comprising a data acquisition module and a spectra processing module, and a data representing module 4.
- the light source can be either a broadband light source or a narrowband light source. If broadband light source is used, either a broadband detector and a filter can be used, or narrowband detectors.
- the optical module is operating in the ultra violet region (wavelength range 190-330 nm).
- FIG. 2 Another embodiment of the invention is shown in Fig. 2.
- the device is applied for determining the concentration of substances such as uric acid during dialysis in spent dialysate 6.
- the device comprises spectrophotometer 7, a signal processing unit 8 for smoothing and derivate calculation, a unit 9 for calculating concentration 10 of the substance in the spent dialysate.
- the measuring cuvette can be, e.g., adapted for in- vitro measurements, or designed for the on-line measurements.
- the spectra processing module is adapted to execute the Savitzky-Golay algorithm for smoothing and calculating the derivate of the measured spectra.
- the spectra processing module may be further adapted to execute a concentration calculation algorithm comprising a transforming function calculating the concentration of certain substance in the biological fluid.
- the data representing module is adapted to execute a program for data representation and comprises or is connected to a data visualization module, e.g., a monitor, a display, or a printing device.
- a data visualization module e.g., a monitor, a display, or a printing device.
- the optical module consisted of a double-beam spectrophotometer (SHIMATSU UV-2401 PC, Japan) with an accuracy of ⁇ 1% on the dialysate samples taken at pre-determined times during dialysis. Spectrophotometric analysis over a wavelength range of 190-380 nm was performed by a cuvette with an optical path length of 1 cm.
- the data acquisition module consisted of a PC incorporated in the spectrophotometer using UV-PC software (UV-PC personal spectrophotometer software, version 3.9 for Windows).
- the obtained UV-absorbance values were processed and presented by a signal processing module using Savitzky-Golay smoothing algorithm, and the derivative calculating algorithm and EXCEL (Microsoft Office Excel 2003) software (concentration calculation algorithm containing the transforming function calculating the concentration of certain substance in the biological fluid).
- the data representing module was either the computer screen or a printer.
- Table 1 Summary results for the different methods to measure concentration of the uric acid.
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Le procédé et le dispositif de l'invention permettent de mesurer la concentration de substances, de préférence, d'acide urique, dans des fluides biologiques. Les mesures sont de type optique et utilisent le spectre du fluide biologique, l'algorithme de Savitzky-Golay, et un algorithme de calcul de la concentration contenant la fonction de transformation pour déterminer la concentration des substances dans des spécimens in vitro ou des fluides en écoulement en continu. Le procédé et le dispositif déterminent la concentration des substances in vitro ou en continu au moyen d'une cuvette de mesure utilisable pour des mesures spécifiées.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EEP201000054A EE05622B1 (et) | 2007-12-04 | 2008-12-04 | Seade ja meetod bioloogilises vedelikus sisalduvate ainete kontsentratsiooni m??tmiseks |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US99215607P | 2007-12-04 | 2007-12-04 | |
| US60/992,156 | 2007-12-04 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2009071102A1 true WO2009071102A1 (fr) | 2009-06-11 |
Family
ID=40428473
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/EE2008/000026 WO2009071102A1 (fr) | 2007-12-04 | 2008-12-04 | Procédé et dispositif optique pour mesurer des concentrations de substances dans des fluides biologiques |
Country Status (2)
| Country | Link |
|---|---|
| EE (1) | EE05622B1 (fr) |
| WO (1) | WO2009071102A1 (fr) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011147425A1 (fr) | 2010-05-27 | 2011-12-01 | Tallinn University Of Technology | Procédé et dispositif de mesure et de surveillance des concentrations de substances dans un liquide biologique |
| WO2012000521A1 (fr) | 2010-06-28 | 2012-01-05 | Tallinn University Of Technology | Procédé et dispositif pour déterminer la teneur en toxines urémiques liées aux protéines et moyennes dans un fluide biologique |
| DE202010017537U1 (de) | 2010-12-16 | 2012-02-09 | Ldiamon As | Nachweisvorrichtung zur Bestimmung des Urea-Gehalts |
| WO2012152514A1 (fr) | 2011-05-11 | 2012-11-15 | Ldiamon As | Procédé et dispositif de dosage pour déterminer le taux d'acide urique et de créatinine |
| EP2942614A1 (fr) | 2014-05-08 | 2015-11-11 | B. Braun Avitum AG | Dispositif et procédé de commande de dispositif destiné à la détermination de concentration quantitative de substances sélectionnées dans un liquide extraites par filtration d'un corps d'un patient |
| CN110411969A (zh) * | 2019-08-02 | 2019-11-05 | 淮阴师范学院 | 紫外分光光度法测定禽类粪便中尿酸含量的方法 |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5351686A (en) * | 1990-10-06 | 1994-10-04 | In-Line Diagnostics Corporation | Disposable extracorporeal conduit for blood constituent monitoring |
| US6674526B1 (en) * | 2000-08-03 | 2004-01-06 | Ralf Marbach | Methods and apparatus for improving the long-term stability of spectroscopic quantitative analyses |
| GB2390420A (en) * | 2002-07-02 | 2004-01-07 | James Samsoondar | Method for calibrating spectrophotometric apparatus |
| US20050119541A1 (en) * | 1999-10-08 | 2005-06-02 | Lorenz Alexander D. | Method of adapting in-vitro models to aid in noninvasive glucose determination |
-
2008
- 2008-12-04 WO PCT/EE2008/000026 patent/WO2009071102A1/fr active Application Filing
- 2008-12-04 EE EEP201000054A patent/EE05622B1/xx not_active IP Right Cessation
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5351686A (en) * | 1990-10-06 | 1994-10-04 | In-Line Diagnostics Corporation | Disposable extracorporeal conduit for blood constituent monitoring |
| US20050119541A1 (en) * | 1999-10-08 | 2005-06-02 | Lorenz Alexander D. | Method of adapting in-vitro models to aid in noninvasive glucose determination |
| US6674526B1 (en) * | 2000-08-03 | 2004-01-06 | Ralf Marbach | Methods and apparatus for improving the long-term stability of spectroscopic quantitative analyses |
| GB2390420A (en) * | 2002-07-02 | 2004-01-07 | James Samsoondar | Method for calibrating spectrophotometric apparatus |
Non-Patent Citations (4)
| Title |
|---|
| FRIDOLIN I ET AL: "ON-LINE MONITORING OF SOLUTES IN DIALYSATE USING ABSORPTION OF ULTRAVIOLET RADIATION: TECHNIQUE DESCRIPTION", INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS, MILAN, IT, vol. 25, no. 8, 1 August 2002 (2002-08-01), pages 748 - 761, XP008101248, ISSN: 0391-3988 * |
| J.T. OLESBERG ET AL.: "Online Measurement of Urea Concentration in Spent Dialysate during Hemodialysis", CLINICAL CHEMISTRY, vol. 50, no. 1, 2004, pages 175 - 181, XP002520388 * |
| SAVITZKY A ET AL: "SMOOTHING AND DIFFERENTIATION OF DATA BY SIMPLIFIED LEAST SQUARES PROCEDURES", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 36, no. 8, 1 July 1964 (1964-07-01), pages 1627 - 1639, XP000560623, ISSN: 0003-2700 * |
| UHLIN F ET AL: "ESTIMATING TOTAL UREA REMOVAL AND PROTEIN CATABOLIC RATE BY MONITORING UV ABSORBANCE IN SPENT DIALYSATE", NEPHROLOGY DIALYSIS TRANSPLANTATION, OXFORD UNIVERSITY PRESS, GB, vol. 20, 1 January 2005 (2005-01-01), pages 2458 - 2464, XP001167226, ISSN: 0931-0509, [retrieved on 20050802] * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011147425A1 (fr) | 2010-05-27 | 2011-12-01 | Tallinn University Of Technology | Procédé et dispositif de mesure et de surveillance des concentrations de substances dans un liquide biologique |
| WO2012000521A1 (fr) | 2010-06-28 | 2012-01-05 | Tallinn University Of Technology | Procédé et dispositif pour déterminer la teneur en toxines urémiques liées aux protéines et moyennes dans un fluide biologique |
| DE202010017537U1 (de) | 2010-12-16 | 2012-02-09 | Ldiamon As | Nachweisvorrichtung zur Bestimmung des Urea-Gehalts |
| WO2012152514A1 (fr) | 2011-05-11 | 2012-11-15 | Ldiamon As | Procédé et dispositif de dosage pour déterminer le taux d'acide urique et de créatinine |
| DE102011101193A1 (de) | 2011-05-11 | 2012-11-15 | Ldiamon As | Verfahren und Nachweisvorrichtung zur Bestimmung des Harnsäure- und Kreatinin-Gehalts |
| EP2942614A1 (fr) | 2014-05-08 | 2015-11-11 | B. Braun Avitum AG | Dispositif et procédé de commande de dispositif destiné à la détermination de concentration quantitative de substances sélectionnées dans un liquide extraites par filtration d'un corps d'un patient |
| DE102014106489A1 (de) | 2014-05-08 | 2015-11-12 | B. Braun Avitum Ag | Vorrichtung und Vorrichtungs-Steuerungsverfahren zur quantitativen Konzentrationsbestimmung ausgewählter aus einem Patientenkörper ausgefilterter Substanzen in einer Flüssigkeit |
| US9423338B2 (en) | 2014-05-08 | 2016-08-23 | B. Braun Avitum Ag | Apparatus and apparatus control method for the quantitative concentration determination of selected substances filtered out of a patient's body in a fluid |
| CN110411969A (zh) * | 2019-08-02 | 2019-11-05 | 淮阴师范学院 | 紫外分光光度法测定禽类粪便中尿酸含量的方法 |
| CN110411969B (zh) * | 2019-08-02 | 2021-10-01 | 淮阴师范学院 | 紫外分光光度法测定禽类粪便中尿酸含量的方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| EE05622B1 (et) | 2013-02-15 |
| EE201000054A (et) | 2010-08-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP4515786B2 (ja) | 全血中の分析対象物の計測に対するヘマトクリットの影響を減らす方法ならびに当該方法で有用な試験キット及び試験用品 | |
| EP1214579B1 (fr) | Procede d'etalonnage d'un dispositif spectroscopique | |
| EP3737938B1 (fr) | Systèmes et procédés de détection et de quantification d'ammoniac et d'ammonium dans des liquides | |
| US7002670B2 (en) | Optical sensor and method for measuring concentration of a chemical constituent using its intrinsic optical absorbance | |
| WO2003016882A1 (fr) | Mesure d'une substance dans un echantillon biologique | |
| JPH0734758B2 (ja) | 全血グルコース決定方法及び該方法を実施するためのキュベット及び光度計 | |
| WO2009071102A1 (fr) | Procédé et dispositif optique pour mesurer des concentrations de substances dans des fluides biologiques | |
| WO2011147425A1 (fr) | Procédé et dispositif de mesure et de surveillance des concentrations de substances dans un liquide biologique | |
| Roth et al. | Infrared spectroscopy in hemodialysis: reagent-free monitoring of patient detoxification by infrared spectroscopy | |
| WO2008037068A1 (fr) | Technique de corrélation pour l'analyse d'un état clinique | |
| JP2009109196A (ja) | 希釈倍率導出方法、定量方法、及び分析装置 | |
| Swati et al. | Nanoengineered optical urea biosensor for estimating hemodialysis parameters in spent dialysate | |
| Jensen et al. | Determination of urea, glucose, and phosphate in dialysate with Fourier transform infrared spectroscopy | |
| Lin et al. | Optoelectronic online monitoring system for hemodialysis and its data analysis | |
| Sudha et al. | Quantification of sweat urea in diabetes using electro-optical technique | |
| BRPI0822570B1 (pt) | Processo in vitro para a determinação da concentração de hemoglobina em uma amostra de sangue diluída em uma só etapa | |
| Vályi-Nagy et al. | Application of near infrared spectroscopy to the determination of haemoglobin | |
| Devi et al. | Analysis on renal failure patients blood samples: characterization and efficacy study | |
| Kondepati et al. | Infrared transmission spectrometry for the determination of urea in microliter sample volumes of blood plasma dialysates | |
| Woo et al. | The advance of technology as a prelude to the laboratory of the twenty-first century | |
| Sbrignadello et al. | Determination of glucose levels during dialysis treatment: different sensors and technologies | |
| Snoer Jensen et al. | Online monitoring of urea concentration in dialysate with dual-beam Fourier-transform near-infrared spectroscopy | |
| US20240110919A1 (en) | A system and method for determining a concentration of an analyte in a fluid | |
| Sulistiyowati et al. | The Comparison of Blood Glucose Check Results Using Photometer and Glucometer | |
| Martinez et al. | On-line measurement of urea in blood using optical spectroscopy in the visible range; validation of the cell shrinkage hypothesis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 08857365 Country of ref document: EP Kind code of ref document: A1 |
|
| NENP | Non-entry into the national phase |
Ref country code: DE |
|
| 122 | Ep: pct application non-entry in european phase |
Ref document number: 08857365 Country of ref document: EP Kind code of ref document: A1 |