WO2009069711A1 - small RNAの取得用担体、取得方法及び取得用試薬 - Google Patents

small RNAの取得用担体、取得方法及び取得用試薬 Download PDF

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Publication number
WO2009069711A1
WO2009069711A1 PCT/JP2008/071576 JP2008071576W WO2009069711A1 WO 2009069711 A1 WO2009069711 A1 WO 2009069711A1 JP 2008071576 W JP2008071576 W JP 2008071576W WO 2009069711 A1 WO2009069711 A1 WO 2009069711A1
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Prior art keywords
small rna
carrier
reagent
obtaining
particle
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PCT/JP2008/071576
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English (en)
French (fr)
Inventor
Takahiro Nishibu
Sohei Funaoka
Yuzo Hamaguchi
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Wako Pure Chemical Industries, Ltd.
Sumitomo Bakelite Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wako Pure Chemical Industries, Ltd., Sumitomo Bakelite Co., Ltd. filed Critical Wako Pure Chemical Industries, Ltd.
Priority to EP08855276.5A priority Critical patent/EP2216406B1/en
Priority to US12/744,797 priority patent/US20100311958A1/en
Publication of WO2009069711A1 publication Critical patent/WO2009069711A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C12N2310/141—MicroRNAs, miRNAs

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　本発明は、（１）核となる粒子の表面に重合性官能基又は連鎖移動基を導入し、該粒子と重合性モノマーを混合し、次いで重合反応を進行させることにより、該粒子表面に高分子化合物を含む層を形成した粒子とし、更にその表面上に、small RNA結合タンパク質に対して親和性を有する生理活性物質を固定化した、small RNA取得用担体、（２）上記担体を用いることを特徴とするsmall RNAの取得方法、（３）上記担体を含んでなるsmall RNA又は／及びsmall RNA標的核酸の取得用試薬、（４）該試薬を含んでなる、small RNA又は／及びsmall RNA標的核酸の取得用試薬キット、及び（５）上記担体を用いることを特徴とするsmall RNA標的核酸の取得方法に関する。
PCT/JP2008/071576 2007-11-30 2008-11-27 small RNAの取得用担体、取得方法及び取得用試薬 WO2009069711A1 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP08855276.5A EP2216406B1 (en) 2007-11-30 2008-11-27 Carrier, method and reagent for obtaining small rna
US12/744,797 US20100311958A1 (en) 2007-11-30 2008-11-27 Carrier, method and reagent for obtaining small rna

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2007311317 2007-11-30
JP2007-311317 2007-11-30
JP2008129068A JP5349838B2 (ja) 2007-11-30 2008-05-16 ｓｍａｌｌＲＮＡの取得用担体、取得方法及び取得用試薬
JP2008-129068 2008-05-16

Publications (1)

Publication Number Publication Date
WO2009069711A1 true WO2009069711A1 (ja) 2009-06-04

Family

ID=40678603

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/071576 WO2009069711A1 (ja) 2007-11-30 2008-11-27 small RNAの取得用担体、取得方法及び取得用試薬

Country Status (4)

Country Link
US (1) US20100311958A1 (ja)
EP (1) EP2216406B1 (ja)
JP (1) JP5349838B2 (ja)
WO (1) WO2009069711A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130197206A1 (en) * 2010-09-24 2013-08-01 Wako Pure Chemical Industries, Ltd. Method for acquisition of small rna

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008087909A1 (ja) * 2007-01-16 2008-07-24 Sumitomo Bakelite Company, Ltd. 医療用粒子、及び分析用粒子、並びにそれらの製造方法
BR112013032232A2 (pt) * 2011-06-16 2016-09-20 Caris Life Sciences Switzerland Holdings S A R L método de caracterização de câncer através do uso de biomarcador de ácido nucleico
JP2013019713A (ja) * 2011-07-08 2013-01-31 Sumitomo Bakelite Co Ltd 遺伝子物質固定化医療用粒子および遺伝子物質の捕捉方法
RU2648910C2 (ru) * 2012-07-06 2018-03-28 ВЕРТЕЛЛУС ХОЛДИНГЗ ЭлЭлСи Композиции на основе сополимера олефина и малеинового ангидрида и их применения
KR102194403B1 (ko) 2013-03-15 2020-12-24 버텔러스 홀딩스 엘엘씨 충격-개질된 폴리아미드 조성물
ES2778223T3 (es) * 2013-11-27 2020-08-10 Sigma Aldrich Co Llc Aislamiento de ARNmicro de fluido biológico
WO2019201277A1 (en) * 2018-04-17 2019-10-24 Wang Mong Lien Method for blocking stress-induced tumor progression

Citations (4)

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JPH0541946B2 (ja) 1983-08-05 1993-06-25 Wako Pure Chem Ind Ltd
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
WO2005029095A1 (ja) * 2003-09-19 2005-03-31 Sumitomo Bakelite Co., Ltd. バイオチップ
WO2007037069A1 (ja) * 2005-09-27 2007-04-05 Sumitomo Bakelite Company, Ltd. 医療用粒子及びその製造方法

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US7459146B2 (en) * 2003-05-30 2008-12-02 3M Innovative Properties Company Stabilized aerosol dispersions
JP4347211B2 (ja) * 2004-12-24 2009-10-21 住友ベークライト株式会社 バイオチップ用基板およびバイオチップ
US7264872B2 (en) * 2004-12-30 2007-09-04 3M Innovative Properties Company Durable high index nanocomposites for AR coatings
JP2006258458A (ja) * 2005-03-15 2006-09-28 Sumitomo Bakelite Co Ltd 医療材料用高分子化合物及び該高分子化合物を用いたバイオチップ用基板
JP5167811B2 (ja) * 2005-05-19 2013-03-21 住友ベークライト株式会社 医療材料用高分子化合物及び該高分子化合物を用いたバイオチップ用基板
WO2008087909A1 (ja) * 2007-01-16 2008-07-24 Sumitomo Bakelite Company, Ltd. 医療用粒子、及び分析用粒子、並びにそれらの製造方法
JP5365623B2 (ja) * 2008-03-11 2013-12-11 住友ベークライト株式会社 生理活性物質の固定化方法

Patent Citations (4)

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JPH0541946B2 (ja) 1983-08-05 1993-06-25 Wako Pure Chem Ind Ltd
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
WO2005029095A1 (ja) * 2003-09-19 2005-03-31 Sumitomo Bakelite Co., Ltd. バイオチップ
WO2007037069A1 (ja) * 2005-09-27 2007-04-05 Sumitomo Bakelite Company, Ltd. 医療用粒子及びその製造方法

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130197206A1 (en) * 2010-09-24 2013-08-01 Wako Pure Chemical Industries, Ltd. Method for acquisition of small rna

Also Published As

Publication number Publication date
EP2216406A4 (en) 2012-01-04
JP2009148243A (ja) 2009-07-09
JP5349838B2 (ja) 2013-11-20
US20100311958A1 (en) 2010-12-09
EP2216406A1 (en) 2010-08-11
EP2216406B1 (en) 2014-01-15

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