WO2009041577A1 - 尿中タンパク質定量用の尿前処理剤、尿前処理方法、及び尿中タンパク質定量方法。 - Google Patents

尿中タンパク質定量用の尿前処理剤、尿前処理方法、及び尿中タンパク質定量方法。 Download PDF

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Publication number
WO2009041577A1
WO2009041577A1 PCT/JP2008/067422 JP2008067422W WO2009041577A1 WO 2009041577 A1 WO2009041577 A1 WO 2009041577A1 JP 2008067422 W JP2008067422 W JP 2008067422W WO 2009041577 A1 WO2009041577 A1 WO 2009041577A1
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WO
WIPO (PCT)
Prior art keywords
urine
protein determination
urinary protein
urine pretreatment
pretreatment agent
Prior art date
Application number
PCT/JP2008/067422
Other languages
English (en)
French (fr)
Inventor
Masanori Hara
Akihiko Saito
Shinya Ogasawara
Yoshiaki Hirayama
Hiroyuki Kurosawa
Original Assignee
Niigata University
Denka Seiken Co. Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Niigata University, Denka Seiken Co. Ltd. filed Critical Niigata University
Priority to EP08833401.6A priority Critical patent/EP2196803B1/en
Priority to JP2009534406A priority patent/JP5515740B2/ja
Priority to US12/680,479 priority patent/US8105840B2/en
Publication of WO2009041577A1 publication Critical patent/WO2009041577A1/ja

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6827—Total protein determination, e.g. albumin in urine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/108331—Preservative, buffer, anticoagulant or diluent

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

尿のｐＨの変動の影響を低減又は解消し、尿中の無機塩が析出した沈降物の影響を解消し、膜タンパク質の可溶化ができる尿前処理剤、尿前処理方法、及び尿中タンパク質定量方法を開発すること。 緩衝剤、キレート剤及び界面活性剤を含むことを特徴とする尿中タンパク質定量用の尿前処理剤と、尿１００質量部に対して前記尿前処理剤１０～１０００質量部を混合することを特徴とする尿前処理方法と、尿１００質量部に対して前記尿前処理剤１０～１０００質量部を混合した後、タンパク質濃度を測定することを特徴とする尿中タンパク質定量方法とを提供する。
PCT/JP2008/067422 2007-09-27 2008-09-26 尿中タンパク質定量用の尿前処理剤、尿前処理方法、及び尿中タンパク質定量方法。 WO2009041577A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP08833401.6A EP2196803B1 (en) 2007-09-27 2008-09-26 Urine pretreatment agent for urinary protein determination, urine pretreatment method, and urinary protein determination method
JP2009534406A JP5515740B2 (ja) 2007-09-27 2008-09-26 尿中タンパク質定量用の尿前処理剤、尿前処理方法、及び尿中タンパク質定量方法。
US12/680,479 US8105840B2 (en) 2007-09-27 2008-09-26 Urine pretreatment agent for urinary protein quantitation, urine pretreatment method, and urinary protein quantitation method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-251707 2007-09-27
JP2007251707 2007-09-27

Publications (1)

Publication Number Publication Date
WO2009041577A1 true WO2009041577A1 (ja) 2009-04-02

Family

ID=40511457

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/067422 WO2009041577A1 (ja) 2007-09-27 2008-09-26 尿中タンパク質定量用の尿前処理剤、尿前処理方法、及び尿中タンパク質定量方法。

Country Status (4)

Country Link
US (1) US8105840B2 (ja)
EP (1) EP2196803B1 (ja)
JP (1) JP5515740B2 (ja)
WO (1) WO2009041577A1 (ja)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010126043A1 (ja) * 2009-04-27 2010-11-04 国立大学法人新潟大学 尿中ヒトメガリンを測定することを含む腎疾患検出方法
US20110195427A1 (en) * 2010-02-10 2011-08-11 Selinfreund Richard H Systems and methods for detecting pregnancy
US20120164667A1 (en) * 2009-06-10 2012-06-28 Masanori Hara Method for test on diabetic nephropathy
CN102713625A (zh) * 2010-01-08 2012-10-03 田中贵金属工业株式会社 免疫层析用试剂组合物
US8628930B2 (en) 2009-04-27 2014-01-14 Niigata University Use of megalin in urine as marker for detecting renal disorder
US8703430B2 (en) 2006-03-28 2014-04-22 Niigata University Method for measuring human megalin
WO2014065312A1 (ja) 2012-10-23 2014-05-01 デンカ生研株式会社 尿を変性剤で前処理することによる免疫測定系の感度を上げる方法
US20160003820A1 (en) * 2012-12-26 2016-01-07 Masanori Hara Method for detecting podocytes in urine

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2721140B1 (en) 2011-06-19 2016-11-23 Abogen, Inc. Devices, solutions and methods for sample collection
AU2015323513B2 (en) 2014-09-05 2021-10-21 American University Of Beirut Determination of risk for development of cardiovascular disease by measuring urinary levels of podocin and nephrin messenger RNA
US10809265B2 (en) 2015-04-22 2020-10-20 Masanori Hara Method for estimating number of podocytes in urine
ES2911415T3 (es) 2015-06-08 2022-05-19 Arquer Diagnostics Ltd Métodos y kits
EP3304082B1 (en) 2015-06-08 2020-05-13 Arquer Diagnostics Limited Methods for analysing a urine sample
EP3557249A4 (en) 2016-12-19 2020-09-30 Yukashikado Inc. URINE TESTING DEVICE AND URINE TESTING PROCEDURE
EP3906319A4 (en) * 2019-01-03 2022-11-16 Hangzhou New Horizon Health Technology Co., Ltd. COMPOSITIONS AND METHODS FOR STORAGE OF UREA SPECIMEN AND EXTRACTION OF DNA
CA3178146A1 (en) * 2020-03-30 2021-10-07 Dna Genotek Inc. Stabilizing composition and method for preserving a bodily fluid
CN115530155A (zh) * 2021-06-29 2022-12-30 成都理想之光科技有限公司 一种24小时尿液样本保存剂、制备方法及应用

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WO1997008549A1 (fr) * 1995-08-31 1997-03-06 Seikagaku Corporation Procede de detection d'affections renales, produit et kit de diagnostic appropries
JPH11153602A (ja) * 1997-09-23 1999-06-08 Bayer Corp 全タンパク質検出法
WO2002037099A1 (fr) * 2000-10-27 2002-05-10 International Reagents Corporation Procede de diagnostic de la nephropathie
JP2007205732A (ja) * 2006-01-31 2007-08-16 Sysmex Corp 粒子分析装置用シース液
WO2007119563A1 (ja) * 2006-03-28 2007-10-25 Niigata University ヒトメガリンの測定方法

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US5385847A (en) * 1993-12-02 1995-01-31 Miles Inc. Method for the determination of urinary protein and creatinine
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* Cited by examiner, † Cited by third party
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WO1997008549A1 (fr) * 1995-08-31 1997-03-06 Seikagaku Corporation Procede de detection d'affections renales, produit et kit de diagnostic appropries
JPH11153602A (ja) * 1997-09-23 1999-06-08 Bayer Corp 全タンパク質検出法
WO2002037099A1 (fr) * 2000-10-27 2002-05-10 International Reagents Corporation Procede de diagnostic de la nephropathie
JP2007205732A (ja) * 2006-01-31 2007-08-16 Sysmex Corp 粒子分析装置用シース液
WO2007119563A1 (ja) * 2006-03-28 2007-10-25 Niigata University ヒトメガリンの測定方法

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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8703430B2 (en) 2006-03-28 2014-04-22 Niigata University Method for measuring human megalin
WO2010126043A1 (ja) * 2009-04-27 2010-11-04 国立大学法人新潟大学 尿中ヒトメガリンを測定することを含む腎疾患検出方法
JP2010256250A (ja) * 2009-04-27 2010-11-11 Niigata Univ 尿中ヒトメガリンを測定することを含む腎疾患検出方法
US8628930B2 (en) 2009-04-27 2014-01-14 Niigata University Use of megalin in urine as marker for detecting renal disorder
US8663931B2 (en) 2009-04-27 2014-03-04 Niigata University Method for detecting renal disease comprising measuring human megalin in urine
US20120164667A1 (en) * 2009-06-10 2012-06-28 Masanori Hara Method for test on diabetic nephropathy
CN102713625A (zh) * 2010-01-08 2012-10-03 田中贵金属工业株式会社 免疫层析用试剂组合物
US20110195427A1 (en) * 2010-02-10 2011-08-11 Selinfreund Richard H Systems and methods for detecting pregnancy
WO2014065312A1 (ja) 2012-10-23 2014-05-01 デンカ生研株式会社 尿を変性剤で前処理することによる免疫測定系の感度を上げる方法
JP2014085208A (ja) * 2012-10-23 2014-05-12 Denka Seiken Co Ltd 尿を変性剤で前処理することによる免疫測定系の感度を上げる方法
US9568470B2 (en) 2012-10-23 2017-02-14 Denka Seiken Co., Ltd. Method for increasing the sensitivity of immunoassay system through pretreatment of urine with denaturant
US20160003820A1 (en) * 2012-12-26 2016-01-07 Masanori Hara Method for detecting podocytes in urine

Also Published As

Publication number Publication date
EP2196803A4 (en) 2010-12-22
JP5515740B2 (ja) 2014-06-11
JPWO2009041577A1 (ja) 2011-01-27
EP2196803B1 (en) 2015-07-29
US8105840B2 (en) 2012-01-31
EP2196803A1 (en) 2010-06-16
US20100233738A1 (en) 2010-09-16

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