WO2009031678A1 - ヒト間葉系幹細胞濃縮方法 - Google Patents

ヒト間葉系幹細胞濃縮方法 Download PDF

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Publication number
WO2009031678A1
WO2009031678A1 PCT/JP2008/066169 JP2008066169W WO2009031678A1 WO 2009031678 A1 WO2009031678 A1 WO 2009031678A1 JP 2008066169 W JP2008066169 W JP 2008066169W WO 2009031678 A1 WO2009031678 A1 WO 2009031678A1
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WIPO (PCT)
Prior art keywords
cells
mesenchymal stem
stem cells
human mesenchymal
mass
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PCT/JP2008/066169
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English (en)
French (fr)
Inventor
Yumi Matsuzaki
Yo Mabuchi
Satoru Morikawa
Hideyuki Okano
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Keio University
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Publication date
Application filed by Keio University filed Critical Keio University
Priority to US12/676,827 priority Critical patent/US20110053183A1/en
Publication of WO2009031678A1 publication Critical patent/WO2009031678A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Rheumatology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

　本発明の目的は、ヒト間葉系幹細胞が含まれる細胞集団から、ヒト間葉系幹細胞を高度に濃縮する方法を提供することである。ヒト間葉系幹細胞を高度に濃縮するには、フローサイトメトリー等を用いて、ヒト間葉系幹細胞が含まれる細胞集団から、CD271+CD90+細胞を回収する。また、その細胞集団に血球細胞が含まれる場合（例えば、骨髄、末梢血等から調製した細胞集団）には、CD45-CD235a-CD271+CD90+細胞を回収する。これらの細胞分画には、自己複製能、自己増殖能、及び多分化能を兼ね備えた間葉系幹細胞が高純度に含まれる。従って、ヒト間葉系幹細胞が含まれる細胞集団から、CD271+CD90+細胞を回収すれば、ヒト間葉系幹細胞を高度に濃縮することができる。
PCT/JP2008/066169 2007-09-06 2008-09-08 ヒト間葉系幹細胞濃縮方法 WO2009031678A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US12/676,827 US20110053183A1 (en) 2007-09-06 2008-09-08 Method of concentrating human mesenchymal stem cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007231298A JP2009060840A (ja) 2007-09-06 2007-09-06 ヒト間葉系幹細胞濃縮方法
JP2007-231298 2007-09-06

Publications (1)

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WO2009031678A1 true WO2009031678A1 (ja) 2009-03-12

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PCT/JP2008/066169 WO2009031678A1 (ja) 2007-09-06 2008-09-08 ヒト間葉系幹細胞濃縮方法

Country Status (3)

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US (1) US20110053183A1 (ja)
JP (1) JP2009060840A (ja)
WO (1) WO2009031678A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010150650A1 (ja) * 2009-06-23 2010-12-29 学校法人日本大学 幹細胞の未分化状態を維持する新規方法
WO2021144995A1 (ja) * 2020-01-16 2021-07-22 ＰｕＲＥＣ株式会社 高純度間葉系幹細胞

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012210154A (ja) * 2009-08-03 2012-11-01 Keio Gijuku 分化細胞由来多能性幹細胞の樹立方法
CA2842181C (en) * 2010-08-27 2021-01-05 University Of Miami Bone marrow derived cd271 precursor cells for cardiac repair
US9555061B2 (en) 2011-04-15 2017-01-31 National University Corporation Torrori University Synthesis and analysis of compounds capable of inducing differentiation of human mesenchymal stems cells into hepatocytes
JP6463029B2 (ja) * 2013-08-02 2019-01-30 有未 伊谷 ヒト間葉系幹細胞を特異的に認識するモノクローナル抗体並びにこれを用いたヒト間葉系幹細胞の分離及び／または品質評価を行う方法
JP6207299B2 (ja) * 2013-08-20 2017-10-04 学校法人関西医科大学 間葉系幹細胞の分離方法
EP3074506A1 (en) 2014-02-12 2016-10-05 National University of Ireland, Galway Selection and use of stem cells
AU2015297347B2 (en) 2014-08-01 2021-05-27 PuREC Co., Ltd Method for evaluating quality of human mesenchymal stem cell, and monoclonal antibody for use in said method
CA3017589A1 (en) * 2016-03-30 2017-10-05 University Of Pittsburgh-Of The Commonwealth System Of Higher Education System for portable gas storage and delivery
CN105866432B (zh) * 2016-05-11 2018-01-26 天津欣普赛尔生物医药科技有限公司 用于鉴定牙髓间充质干细胞的试剂盒
US11613733B2 (en) 2017-05-12 2023-03-28 Medical & Biological Laboratories Co., Ltd. Method for purifying mesenchymal stem cells to improve transplantation efficiency

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9441244B2 (en) * 2003-06-30 2016-09-13 The Regents Of The University Of California Mutant adeno-associated virus virions and methods of use thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
COVAS DT ET AL.: "Isolation and culture of umbilical vein mesenchymal stem cells.", BRAZ J MED BIOL RES., vol. 36, no. 9, 2003, pages 1179 - 1183, XP002427643 *
NAOKI YAMAMOTO ET AL.: "Isolation of multipotent stem cells from mouse adipose tissue.", JOURNAL OF DERMATOLOGICAL SCIENCE, vol. 48, no. 1, 31 August 2007 (2007-08-31), pages 43 - 52, XP022209304 *
QUIRICI N ET AL.: "Isolation of bone marrow mesenchymal stem cells by anti-nerve growth factor receptor antibodies.", EXP HEMATOL., vol. 30, no. 7, 2002, pages 783 - 791, XP002973273 *
YOUNG HE ET AL.: "Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors.", ANAT REC., vol. 264, no. 1, 2001, pages 51 - 62, XP055069134 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010150650A1 (ja) * 2009-06-23 2010-12-29 学校法人日本大学 幹細胞の未分化状態を維持する新規方法
JP2011004607A (ja) * 2009-06-23 2011-01-13 Nihon Univ 幹細胞の未分化状態を維持する新規方法
WO2021144995A1 (ja) * 2020-01-16 2021-07-22 ＰｕＲＥＣ株式会社 高純度間葉系幹細胞
WO2021145002A1 (ja) 2020-01-16 2021-07-22 ＰｕＲＥＣ株式会社 高純度間葉系幹細胞
JPWO2021145002A1 (ja) * 2020-01-16 2021-07-22
JP7466218B2 (ja) 2020-01-16 2024-04-12 ＰｕＲＥＣ株式会社 高純度間葉系幹細胞

Also Published As

Publication number Publication date
JP2009060840A (ja) 2009-03-26
US20110053183A1 (en) 2011-03-03

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