WO2009028336A1 - 一分子蛍光分析によるｓｉＲＮＡの濃度測定方法 - Google Patents

一分子蛍光分析によるｓｉＲＮＡの濃度測定方法 Download PDF

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Publication number
WO2009028336A1
WO2009028336A1 PCT/JP2008/064603 JP2008064603W WO2009028336A1 WO 2009028336 A1 WO2009028336 A1 WO 2009028336A1 JP 2008064603 W JP2008064603 W JP 2008064603W WO 2009028336 A1 WO2009028336 A1 WO 2009028336A1
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Prior art keywords
sirna
fluorometry
concentration
single molecule
test solution
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Ceased
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PCT/JP2008/064603
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English (en)
French (fr)
Inventor
Takashi Abe
Kazuhito Goda
Kazunobu Futami
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Olympus Corp
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Olympus Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

　本発明は、ｓｉＲＮＡを投与した生体試料から調製された核酸抽出物を含む被検溶液において、ｓｉＲＮＡの二本鎖を解離させ、その後、解離した一本鎖のｓｉＲＮＡに、ｓｉＲＮＡの二本鎖の一方の鎖に相補的な配列を含む蛍光標識プローブＤＮＡをハイブリダイズさせる第一の工程と、 第一の工程後の被検溶液に対して一分子蛍光分析を行い、分析値を取得する第二の工程と、既知の種々の濃度のｓｉＲＮＡを含む基準溶液を用いて作成された検量線に基いて、第二の工程で取得した分析値からｓｉＲＮＡの濃度を算出する第三の工程とを含む、一分子蛍光分析によるｓｉＲＮＡの濃度測定方法に関する。本発明によれば、高い精度、簡便かつ安価でｓｉＲＮＡの濃度を測定することができる。
PCT/JP2008/064603 2007-08-24 2008-08-14 一分子蛍光分析によるｓｉＲＮＡの濃度測定方法 Ceased WO2009028336A1 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007218770A JP2010256011A (ja) 2007-08-24 2007-08-24 一分子蛍光分析によるｓｉＲＮＡの濃度測定方法
JP2007-218770 2007-08-24

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WO2009028336A1 true WO2009028336A1 (ja) 2009-03-05

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PCT/JP2008/064603 Ceased WO2009028336A1 (ja) 2007-08-24 2008-08-14 一分子蛍光分析によるｓｉＲＮＡの濃度測定方法

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JP (1) JP2010256011A (ja)
WO (1) WO2009028336A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010227003A (ja) * 2009-03-27 2010-10-14 Jikei Univ 核酸定量法、及び核酸定量装置

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003275000A (ja) * 2002-03-26 2003-09-30 Olympus Optical Co Ltd 一分子蛍光分析により核酸と核酸結合タンパク質との結合を検出する方法
JP2006067914A (ja) * 2004-09-02 2006-03-16 Toshiba Corp 短鎖核酸の検出方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003275000A (ja) * 2002-03-26 2003-09-30 Olympus Optical Co Ltd 一分子蛍光分析により核酸と核酸結合タンパク質との結合を検出する方法
JP2006067914A (ja) * 2004-09-02 2006-03-16 Toshiba Corp 短鎖核酸の検出方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
REMAUT K. ET AL.: "Can we better understand the intracellular behaviour of DNA nanoparticles by fluoroscence correlation spectroscopy?", J.CONTROL RELEASE, vol. 121, no. 1-2, 16 August 2007 (2007-08-16), pages 49 - 63 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010227003A (ja) * 2009-03-27 2010-10-14 Jikei Univ 核酸定量法、及び核酸定量装置

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