WO2009011297A1 - Ｊａｋ２遺伝子の変異検出用プローブおよびその用途 - Google Patents

Ｊａｋ２遺伝子の変異検出用プローブおよびその用途 Download PDF

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Publication number
WO2009011297A1
WO2009011297A1 PCT/JP2008/062548 JP2008062548W WO2009011297A1 WO 2009011297 A1 WO2009011297 A1 WO 2009011297A1 JP 2008062548 W JP2008062548 W JP 2008062548W WO 2009011297 A1 WO2009011297 A1 WO 2009011297A1
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WIPO (PCT)
Prior art keywords
mutation
probe
jak2 gene
sequence
detected
Prior art date
Application number
PCT/JP2008/062548
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English (en)
French (fr)
Inventor
Mitsuharu Hirai
Satoshi Majima
Taira Maekawa
Shinya Kimura
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Arkray, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arkray, Inc. filed Critical Arkray, Inc.
Priority to JP2008553963A priority Critical patent/JPWO2009011297A1/ja
Priority to EP08791078.2A priority patent/EP2119796B1/en
Priority to US12/513,642 priority patent/US20100068713A1/en
Priority to CN200880006541.8A priority patent/CN101622363B/zh
Priority to KR1020087032135A priority patent/KR101147277B1/ko
Publication of WO2009011297A1 publication Critical patent/WO2009011297A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plasma & Fusion (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　一塩基のみが異なる、変異を含む検出対象配列と変異を含まない非検出対象配列とが共存する場合でも、前記変異を含む検出対象配列を検出可能なＪＡＫ２遺伝子変異の検出用プローブを提供する。 　配列番号１の塩基配列からなるＪＡＫ２遺伝子のｅｘｏｎ１２における８４番目のシトシン塩基（Ｃ）を一塩基目として５’方向に向かって１７～２２塩基目までの領域と同じ配列である少なくとも一つのオリゴヌクレオチドであって、前記シトシン塩基（Ｃ）を３’末端とするオリゴヌクレオチドをプローブとして使用する。これらのプローブを、例えば、Ｔｍ解析に使用することによって、変異が発生しているＪＡＫ２遺伝子と変異が発生していないＪＡＫ２遺伝子とが含まれる試料であっても、前者の変異を検出することができる。前記プローブは、蛍光色素で標識化されていることが好ましい。
PCT/JP2008/062548 2007-07-13 2008-07-11 Ｊａｋ２遺伝子の変異検出用プローブおよびその用途 WO2009011297A1 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2008553963A JPWO2009011297A1 (ja) 2007-07-13 2008-07-11 Ｊａｋ２遺伝子の変異検出用プローブおよびその用途
EP08791078.2A EP2119796B1 (en) 2007-07-13 2008-07-11 Probe for detecting mutation in jak2 gene and use thereof
US12/513,642 US20100068713A1 (en) 2007-07-13 2008-07-11 Probe for detecting mutation in jak2 gene and use thereof
CN200880006541.8A CN101622363B (zh) 2007-07-13 2008-07-11 用于检测jak2基因的突变的探针及其用途
KR1020087032135A KR101147277B1 (ko) 2007-07-13 2008-07-11 Ｊａｋ２ 유전자의 변이 검출용 프로브 및 그 용도

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-184842 2007-07-13
JP2007184842 2007-07-13

Publications (1)

Publication Number Publication Date
WO2009011297A1 true WO2009011297A1 (ja) 2009-01-22

Family

ID=40259630

Family Applications (1)

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PCT/JP2008/062548 WO2009011297A1 (ja) 2007-07-13 2008-07-11 Ｊａｋ２遺伝子の変異検出用プローブおよびその用途

Country Status (6)

Country Link
US (1) US20100068713A1 (ja)
EP (1) EP2119796B1 (ja)
JP (1) JPWO2009011297A1 (ja)
KR (1) KR101147277B1 (ja)
CN (1) CN101622363B (ja)
WO (1) WO2009011297A1 (ja)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011244815A (ja) * 2010-04-30 2011-12-08 Arkray Inc ＪＡＫ２遺伝子のｅｘｏｎ１２における変異の検出法、ならびにそのための核酸プローブおよびキット
JP2013017395A (ja) * 2011-07-07 2013-01-31 Toyobo Co Ltd 骨髄増殖性疾患に関する遺伝子変異を検出するためのプローブおよび該プローブを用いた遺伝子変異の検出方法
JP2014528721A (ja) * 2011-09-30 2014-10-30 エピスタム リミテッド Ｊａｋ２の突然変異解析
JP2016073304A (ja) * 2010-10-29 2016-05-12 アークレイ株式会社 Ｅｇｆｒ遺伝子の多型検出用プローブ、増幅用プライマーおよびその用途

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103243153B (zh) * 2012-02-10 2014-11-05 复旦大学附属华山医院 Jak2 v617f突变的检测应用
CN103571959B (zh) * 2013-11-04 2015-07-15 北京海思特临床检验所有限公司 用于检测基因位点突变的引物、探针、试剂盒
CN116234903A (zh) * 2020-11-18 2023-06-06 xFOREST制药株式会社 用于突变谱分析的rna探针及其用途

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005287335A (ja) * 2004-03-31 2005-10-20 Arkray Inc Ｍｄｒ１遺伝子の変異の検出法ならびにそのための核酸プローブおよびキット
JP2005328712A (ja) * 2004-05-18 2005-12-02 Arkray Inc Ｃｙｐ２ｄ６＊４の変異の検出法ならびにそのための核酸プローブおよびキット
JP2005328707A (ja) * 2004-05-18 2005-12-02 Arkray Inc Ｎａｔ２＊６の変異の検出法ならびにそのための核酸プローブおよびキット

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7019129B1 (en) * 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
ES2267803T3 (es) * 2000-08-11 2007-03-16 University Of Utah Research Foundation Sondas de oligonucleotidos marcados individuales.
JP4454249B2 (ja) * 2003-04-18 2010-04-21 アークレイ株式会社 膵ラ氏島アミロイドタンパク質変異遺伝子の検出法ならびにそのための核酸プローブおよびキット
FR2877013A1 (fr) * 2004-10-27 2006-04-28 Assist Publ Hopitaux De Paris Indentification d'une mutation de jak2 impliquee dans la polyglobulie de vaquez

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005287335A (ja) * 2004-03-31 2005-10-20 Arkray Inc Ｍｄｒ１遺伝子の変異の検出法ならびにそのための核酸プローブおよびキット
JP2005328712A (ja) * 2004-05-18 2005-12-02 Arkray Inc Ｃｙｐ２ｄ６＊４の変異の検出法ならびにそのための核酸プローブおよびキット
JP2005328707A (ja) * 2004-05-18 2005-12-02 Arkray Inc Ｎａｔ２＊６の変異の検出法ならびにそのための核酸プローブおよびキット

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
See also references of EP2119796A4 *
STEENSMA P.D.: "JAK2 V617F in Myeloid Disorders: Molecular Diagnostic Techniques and Their Clinical Utility", J. MOL. DIAGN., vol. 8, no. 4, 2006, pages 397 - 411, XP008110066 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011244815A (ja) * 2010-04-30 2011-12-08 Arkray Inc ＪＡＫ２遺伝子のｅｘｏｎ１２における変異の検出法、ならびにそのための核酸プローブおよびキット
JP2016073304A (ja) * 2010-10-29 2016-05-12 アークレイ株式会社 Ｅｇｆｒ遺伝子の多型検出用プローブ、増幅用プライマーおよびその用途
JP2013017395A (ja) * 2011-07-07 2013-01-31 Toyobo Co Ltd 骨髄増殖性疾患に関する遺伝子変異を検出するためのプローブおよび該プローブを用いた遺伝子変異の検出方法
JP2014528721A (ja) * 2011-09-30 2014-10-30 エピスタム リミテッド Ｊａｋ２の突然変異解析

Also Published As

Publication number Publication date
KR20090090263A (ko) 2009-08-25
CN101622363A (zh) 2010-01-06
JPWO2009011297A1 (ja) 2010-09-24
KR101147277B1 (ko) 2012-05-21
EP2119796B1 (en) 2014-01-15
US20100068713A1 (en) 2010-03-18
CN101622363B (zh) 2012-09-05
EP2119796A1 (en) 2009-11-18
EP2119796A4 (en) 2011-03-30

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