WO2009011125A1 - Nouvelle isomaltooligosaccharide synthase - Google Patents

Nouvelle isomaltooligosaccharide synthase Download PDF

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Publication number
WO2009011125A1
WO2009011125A1 PCT/JP2008/001913 JP2008001913W WO2009011125A1 WO 2009011125 A1 WO2009011125 A1 WO 2009011125A1 JP 2008001913 W JP2008001913 W JP 2008001913W WO 2009011125 A1 WO2009011125 A1 WO 2009011125A1
Authority
WO
WIPO (PCT)
Prior art keywords
isomaltooligosaccharide
glucose residues
synthase
novel
isomaltooligosaccharide synthase
Prior art date
Application number
PCT/JP2008/001913
Other languages
English (en)
Japanese (ja)
Inventor
Atsuo Kimura
Mikihiko Kobayashi
Michiyo Kanegae
Hiroyuki Nakai
Masayuki Okuyama
Haruhide Mori
Young-Min Kim
Original Assignee
National University Corporation Hokkaido University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National University Corporation Hokkaido University filed Critical National University Corporation Hokkaido University
Priority to JP2009523542A priority Critical patent/JPWO2009011125A1/ja
Publication of WO2009011125A1 publication Critical patent/WO2009011125A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

On vise à proposer une protéine enzyme qui permet de synthétiser un isomaltooligosaccharide à longue chaîne ayant un grand nombre de restes glucose et un procédé de fabrication d'un isomaltooligosaccharide à l'aide de celle-ci. L'invention porte sur une isomaltooligosaccharide synthase ayant des caractéristiques enzymatiques de : a) être produite par une bactérie du genre Bacillus ; b) ayant une activité d'extension de la chaîne α-1, 6-glucane d'un isomaltooligosaccharide ; et c) synthétiser un isomaltooligosaccharide ayant 5 à 12 restes glucose à partir d'un isomaltooligosaccharide ayant 3,4 ou 7 restes glucose. L'isomaltooligosaccharide synthase de l'invention permet de produire l'isomaltooligosaccharide ayant 5 à 12 restes glucose à un rendement de transfert extrêmement élevé de 90% ou plus, en pratique de plus de 99%.
PCT/JP2008/001913 2007-07-19 2008-07-17 Nouvelle isomaltooligosaccharide synthase WO2009011125A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2009523542A JPWO2009011125A1 (ja) 2007-07-19 2008-07-17 新規イソマルトオリゴ糖合成酵素

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-188298 2007-07-19
JP2007188298 2007-07-19

Publications (1)

Publication Number Publication Date
WO2009011125A1 true WO2009011125A1 (fr) 2009-01-22

Family

ID=40259471

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/001913 WO2009011125A1 (fr) 2007-07-19 2008-07-17 Nouvelle isomaltooligosaccharide synthase

Country Status (2)

Country Link
JP (1) JPWO2009011125A1 (fr)
WO (1) WO2009011125A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017110517A1 (fr) * 2015-12-21 2017-06-29 国立大学法人北海道大学 Isomaltomégalosaccharides de type à double ancre et à ancre simple, procédés pour produire respectivement lesdits composés et utilisation desdits composés
CN109456920A (zh) * 2018-11-30 2019-03-12 江苏大学 一株提高鱼酱发酵品质的中度嗜盐菌菌株海水芽孢杆菌

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005080515A (ja) * 2003-09-04 2005-03-31 Suntory Ltd 新規α−グルコシダーゼ及びその製造法
WO2006054474A1 (fr) * 2004-11-17 2006-05-26 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Dextranase de la dextrine, procede pour la produire et utilisation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005080515A (ja) * 2003-09-04 2005-03-31 Suntory Ltd 新規α−グルコシダーゼ及びその製造法
WO2006054474A1 (fr) * 2004-11-17 2006-05-26 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Dextranase de la dextrine, procede pour la produire et utilisation

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
"Proline Residues Responsible for Thermostability Occur with High Frequency in the Loop Regions of an Extremely Thermostable Oligo-1,6-glucosidase from Bacillus thermoglucosidase KP1006", J. BIOL. CHEM., vol. 226, no. 36, 1991, pages 24287 - 24294, XP002159354 *
"Structual elements in dextran glucosidase responsible for high specificity to long chain substrate", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1764, 2006, pages 688 - 698, XP005413136 *
"The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pX01", NUCLEIC ACIDS RES., vol. 32, no. 3, 2004, pages 977 - 988 *
"The Refined Crystal Structure of Bacillus cereus Oligo-1,6-glucosidase at 2.0A Resolution: Structurual Characterization of Proline-substitution Site for Protein Thermostabilization", J. MOL. BIOL., vol. 269, 1997, pages 142 - 153, XP004461424 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017110517A1 (fr) * 2015-12-21 2017-06-29 国立大学法人北海道大学 Isomaltomégalosaccharides de type à double ancre et à ancre simple, procédés pour produire respectivement lesdits composés et utilisation desdits composés
JP2017114943A (ja) * 2015-12-21 2017-06-29 国立大学法人北海道大学 ダブル及びシングルアンカー型イソマルトメガロ糖、その製造方法及びその利用
CN109456920A (zh) * 2018-11-30 2019-03-12 江苏大学 一株提高鱼酱发酵品质的中度嗜盐菌菌株海水芽孢杆菌
CN109456920B (zh) * 2018-11-30 2021-09-10 江苏大学 一株提高鱼酱发酵品质的中度嗜盐菌菌株海水芽孢杆菌

Also Published As

Publication number Publication date
JPWO2009011125A1 (ja) 2010-09-16

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