WO2008152888A1 - 微生物の精製方法及び微生物の検出方法 - Google Patents

微生物の精製方法及び微生物の検出方法 Download PDF

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Publication number
WO2008152888A1
WO2008152888A1 PCT/JP2008/059333 JP2008059333W WO2008152888A1 WO 2008152888 A1 WO2008152888 A1 WO 2008152888A1 JP 2008059333 W JP2008059333 W JP 2008059333W WO 2008152888 A1 WO2008152888 A1 WO 2008152888A1
Authority
WO
WIPO (PCT)
Prior art keywords
microorganisms
purifying
sample solution
blood preparation
detecting
Prior art date
Application number
PCT/JP2008/059333
Other languages
English (en)
French (fr)
Inventor
Yasuo Motoyama
Mami Matsumoto
Original Assignee
Asahi Breweries, Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asahi Breweries, Ltd. filed Critical Asahi Breweries, Ltd.
Priority to JP2009519202A priority Critical patent/JPWO2008152888A1/ja
Publication of WO2008152888A1 publication Critical patent/WO2008152888A1/ja

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/08—Plasma substitutes; Perfusion solutions; Dialytics or haemodialytics; Drugs for electrolytic or acid-base disorders, e.g. hypovolemic shock
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)

Abstract

本発明は、一度のフィルター濾過操作により、迅速かつ高精度に血液製剤中の生きている微生物を精製する方法、及び、該方法により精製された微生物を検出する方法を提供することを目的とする。本発明は、（ａ）キレート剤を含まない血液製剤に対して、ポリオキシエチレンアルキルエーテルとタンパク質分解酵素とを添加し、試料溶液を調製する工程；（ｂ）前記試料溶液を、前記タンパク質分解酵素に適する条件下でインキュベートし、タンパク質分解反応を行う工程；（ｃ）微生物を通さないフィルターで、当該タンパク質分解反応済試料溶液を濾過し、当該フィルター上に微生物を捕集する工程；を有する、血液製剤中の微生物の精製方法を提供する。
PCT/JP2008/059333 2007-06-11 2008-05-21 微生物の精製方法及び微生物の検出方法 WO2008152888A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2009519202A JPWO2008152888A1 (ja) 2007-06-11 2008-05-21 微生物の精製方法及び微生物の検出方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007153963 2007-06-11
JP2007-153963 2007-06-11

Publications (1)

Publication Number Publication Date
WO2008152888A1 true WO2008152888A1 (ja) 2008-12-18

Family

ID=40129500

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/059333 WO2008152888A1 (ja) 2007-06-11 2008-05-21 微生物の精製方法及び微生物の検出方法

Country Status (2)

Country Link
JP (1) JPWO2008152888A1 (ja)
WO (1) WO2008152888A1 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012507288A (ja) * 2008-11-03 2012-03-29 ゼネラル・エレクトリック・カンパニイ 水性媒体中の微生物含有量の測定方法及びシステム
JP2015527063A (ja) * 2012-07-09 2015-09-17 バイオカーティス エヌ ヴイ 生物サンプルをろ過するための方法
US11261476B2 (en) 2016-10-19 2022-03-01 Q-Linea Ab Method for recovering microbial cells

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04267933A (ja) * 1991-02-25 1992-09-24 Fuji Photo Film Co Ltd 分離膜洗浄方法
WO2006095697A1 (ja) * 2005-03-10 2006-09-14 Bussan Nanotech Research Institute, Inc. 微生物検出方法及び溶菌試薬
JP2007014239A (ja) * 2005-07-06 2007-01-25 Matsushita Electric Ind Co Ltd 微生物検出方法および検出装置
JP2008118899A (ja) * 2006-11-10 2008-05-29 Asahi Breweries Ltd 微生物精製方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04267933A (ja) * 1991-02-25 1992-09-24 Fuji Photo Film Co Ltd 分離膜洗浄方法
WO2006095697A1 (ja) * 2005-03-10 2006-09-14 Bussan Nanotech Research Institute, Inc. 微生物検出方法及び溶菌試薬
JP2007014239A (ja) * 2005-07-06 2007-01-25 Matsushita Electric Ind Co Ltd 微生物検出方法および検出装置
JP2008118899A (ja) * 2006-11-10 2008-05-29 Asahi Breweries Ltd 微生物精製方法

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012507288A (ja) * 2008-11-03 2012-03-29 ゼネラル・エレクトリック・カンパニイ 水性媒体中の微生物含有量の測定方法及びシステム
JP2015527063A (ja) * 2012-07-09 2015-09-17 バイオカーティス エヌ ヴイ 生物サンプルをろ過するための方法
US11261476B2 (en) 2016-10-19 2022-03-01 Q-Linea Ab Method for recovering microbial cells

Also Published As

Publication number Publication date
JPWO2008152888A1 (ja) 2010-08-26

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