WO2008146686A1 - 酵素含有カプセル及び核酸の増幅キット - Google Patents

酵素含有カプセル及び核酸の増幅キット Download PDF

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Publication number
WO2008146686A1
WO2008146686A1 PCT/JP2008/059375 JP2008059375W WO2008146686A1 WO 2008146686 A1 WO2008146686 A1 WO 2008146686A1 JP 2008059375 W JP2008059375 W JP 2008059375W WO 2008146686 A1 WO2008146686 A1 WO 2008146686A1
Authority
WO
WIPO (PCT)
Prior art keywords
enzyme
nucleic acid
containing capsule
acid amplification
amplification kit
Prior art date
Application number
PCT/JP2008/059375
Other languages
English (en)
French (fr)
Inventor
Ken Takubo
Original Assignee
Olympus Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Corporation filed Critical Olympus Corporation
Priority to JP2009516270A priority Critical patent/JPWO2008146686A1/ja
Publication of WO2008146686A1 publication Critical patent/WO2008146686A1/ja
Priority to US12/625,485 priority patent/US20100081176A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

本発明は、試料からタンパク質を失活させて該試料中の核酸を増幅するために用いられる酵素含有カプセル、該酵素含有カプセルを有する核酸の増幅キット、及び該酵素含有カプセルを用いた核酸の増幅方法を提供することを課題とする。本発明は、融点が６０～９５°Cであり、非タンパク質性物質を皮膜成分とし、内部に耐熱性酵素を含有することを特徴とする酵素含有カプセル、前記酵素含有カプセルとタンパク質分解酵素とを有する核酸の増幅キット、及び前記酵素含有カプセルを用いる核酸の増幅方法を提供する。
PCT/JP2008/059375 2007-05-25 2008-05-21 酵素含有カプセル及び核酸の増幅キット WO2008146686A1 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2009516270A JPWO2008146686A1 (ja) 2007-05-25 2008-05-21 酵素含有カプセル及び核酸の増幅キット
US12/625,485 US20100081176A1 (en) 2007-05-25 2009-11-24 Enzyme-containing capsules and nucleic acid amplification kits

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007139143 2007-05-25
JP2007-139143 2007-05-25

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/625,485 Continuation US20100081176A1 (en) 2007-05-25 2009-11-24 Enzyme-containing capsules and nucleic acid amplification kits

Publications (1)

Publication Number Publication Date
WO2008146686A1 true WO2008146686A1 (ja) 2008-12-04

Family

ID=40316828

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/059375 WO2008146686A1 (ja) 2007-05-25 2008-05-21 酵素含有カプセル及び核酸の増幅キット

Country Status (3)

Country Link
US (1) US20100081176A1 (ja)
JP (1) JPWO2008146686A1 (ja)
WO (1) WO2008146686A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9045611B2 (en) 2011-03-04 2015-06-02 Cryovac, Inc. Process for preparing a heat resistant polylactic acid foamed article
JP2020078337A (ja) * 2016-04-20 2020-05-28 Ｂｌｕｅ Ｉｎｄｕｓｔｒｉｅｓ株式会社 遺伝子解析用前処理キット、核酸分析用チップ、解析システム、生体物質分析用チップ

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369445A2 (en) * 1988-11-17 1990-05-23 D.M. Graham Laboratories, Inc. Solid encapsulated medicament and process and apparatus for preparing same
WO1997035537A1 (en) * 1996-03-26 1997-10-02 Bioprogress Technology Limited Improvements in or relating to encapsulation
JP2001212449A (ja) * 2000-02-01 2001-08-07 Kureha Chem Ind Co Ltd カプセルの製造方法
JP2004315466A (ja) * 2003-04-18 2004-11-11 Sansho Pharmaceutical Co Ltd カプセル用皮膜組成物
WO2005102291A1 (ja) * 2004-04-21 2005-11-03 Kyowa Hakko Kogyo Co., Ltd. 水溶性活性成分含有シームレスカプセル

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605824A (en) * 1989-10-24 1997-02-25 Stratagene Composition for hybridizing nucleic acids using single-stranded nucleic acid binding protein
DE69131891T2 (de) * 1990-02-16 2000-06-15 Hoffmann La Roche Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion
JP4102459B2 (ja) * 1997-05-14 2008-06-18 森下仁丹株式会社 生体高分子を合成するシームレスカプセルおよびその製造方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369445A2 (en) * 1988-11-17 1990-05-23 D.M. Graham Laboratories, Inc. Solid encapsulated medicament and process and apparatus for preparing same
WO1997035537A1 (en) * 1996-03-26 1997-10-02 Bioprogress Technology Limited Improvements in or relating to encapsulation
JP2001212449A (ja) * 2000-02-01 2001-08-07 Kureha Chem Ind Co Ltd カプセルの製造方法
JP2004315466A (ja) * 2003-04-18 2004-11-11 Sansho Pharmaceutical Co Ltd カプセル用皮膜組成物
WO2005102291A1 (ja) * 2004-04-21 2005-11-03 Kyowa Hakko Kogyo Co., Ltd. 水溶性活性成分含有シームレスカプセル

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SETTERQUIST R.A. ET AL.: "Ready to use agarose encapsulated PCR reagents", NUCLEIC ACIDS RESEARCH, vol. 24, no. 8, 1996, pages 1580 - 1581 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9045611B2 (en) 2011-03-04 2015-06-02 Cryovac, Inc. Process for preparing a heat resistant polylactic acid foamed article
JP2020078337A (ja) * 2016-04-20 2020-05-28 Ｂｌｕｅ Ｉｎｄｕｓｔｒｉｅｓ株式会社 遺伝子解析用前処理キット、核酸分析用チップ、解析システム、生体物質分析用チップ

Also Published As

Publication number Publication date
JPWO2008146686A1 (ja) 2010-08-19
US20100081176A1 (en) 2010-04-01

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