WO2008145457A1 - Colonne capillaire perfectionnée pour chromatographie en phase gazeuse - Google Patents

Colonne capillaire perfectionnée pour chromatographie en phase gazeuse Download PDF

Info

Publication number
WO2008145457A1
WO2008145457A1 PCT/EP2008/054807 EP2008054807W WO2008145457A1 WO 2008145457 A1 WO2008145457 A1 WO 2008145457A1 EP 2008054807 W EP2008054807 W EP 2008054807W WO 2008145457 A1 WO2008145457 A1 WO 2008145457A1
Authority
WO
WIPO (PCT)
Prior art keywords
gas chromatography
column
stationary phases
different
chromatography column
Prior art date
Application number
PCT/EP2008/054807
Other languages
English (en)
Inventor
Mario Galli
Stefano Galli
Original Assignee
Dani Instruments S.P.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dani Instruments S.P.A. filed Critical Dani Instruments S.P.A.
Publication of WO2008145457A1 publication Critical patent/WO2008145457A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6052—Construction of the column body
    • G01N30/6069—Construction of the column body with compartments or bed substructure
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6052—Construction of the column body
    • G01N30/6073—Construction of the column body in open tubular form
    • G01N30/6078—Capillaries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6052—Construction of the column body
    • G01N30/6082—Construction of the column body transparent to radiation

Definitions

  • the present invention relates to an improved capillary column for gas chromatography.
  • GC Gas chromatography
  • Traditional gas chromatography is also known to be used for analysis and separation (total or partial) of a mixture of components flowing within an inert carrier gas (generally helium, hydrogen or nitrogen), via a chromatography column containing a stationary phase.
  • an inert carrier gas generally helium, hydrogen or nitrogen
  • the instrument used for gas chromatography consists of a gas chromatograph, the main components of which are the sample introduction system, the heated chamber containing the column, and the detection system.
  • Gas chromatography currently uses capillary columns consisting of a fused silica, metal, glass or other material tube of very small inner diameter (typically from 0.05 mm to 0.53 mm), on the inner surface of which a constant thickness film of polymer material is deposited, to form the stationary phase. This thickness is generally between 0.05 ⁇ m and 5 ⁇ m depending on the column type, and is constant throughout the length of the column.
  • the different mixture components have different affinities with the specific stationary phase of the column, they travel within the tube at different velocities, for which reason they can be separated along the gas chromatography column.
  • the chemical composition of the stationary phase determines the types of components which can be separated by the gas chromatography column.
  • Typical components of stationary phases are OV-1 , SE-1 (silicone based) or Carbowax 2OM (polyethyleneglycol based).
  • the stationary phase hence depends on the substances to be analyzed and separated, on their chemical nature and in particular their volatility and polarity.
  • Another drawback consists of the fact that when the glass connectors are subjected to thermal and mechanical stresses (due for example to the modulation systems applied to the 2DGC), the handling of the connected columns becomes problematic and can lead to their mutual disconnection. This sudden disconnection can itself have serious drawbacks both from the analytical and instrumental viewpoints.
  • a column which no longer receives carrier gas and is at high temperature can easily degrade and irremediably lose its separative capacity, and likewise any mass spectrometer coupled to the gas chromatograph in the GC-MS method can be seriously damaged if it no longer receives carrier gas.
  • a preferred embodiment of the present invention is further clarified hereinafter with reference to the accompanying drawing, showing a schematic longitudinal section through a portion of a gas chromatography column according to the invention.
  • the gas chromatography column of the invention comprises a single continuous capillary tube 2 made of fused silica, metal or other material, in which two or more separate stationary phases 4, 6 of different nature and/or different thicknesses are deposited.
  • the invention also includes all possible stationary phase combinations, i.e. stationary phases of different substances or of the same substance but of different thicknesses, or different substances of different thicknesses. These can be positioned one following the other without discontinuities, or can be separated by a portion of column 2 without stationary phases.
  • the capillary column consists of two separate portions of different outer diameter, each of which presents one stationary phase, the two stationary phases being of different substances and/or thicknesses, as in the preceding example.

Abstract

L'invention concerne une colonne capillaire perfectionnée pour chromatographie en phase gazeuse, caractérisée par le fait qu'elle consiste en un seul tube capillaire (2) présentant au moins deux phases immobiles (4, 6) de caractéristiques différentes, mutuellement distinctes le long du tube.
PCT/EP2008/054807 2007-06-01 2008-04-21 Colonne capillaire perfectionnée pour chromatographie en phase gazeuse WO2008145457A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITVE2007A000034 2007-06-01
IT000034A ITVE20070034A1 (it) 2007-06-01 2007-06-01 Colonna capillare perfezionata per gascromatografia.

Publications (1)

Publication Number Publication Date
WO2008145457A1 true WO2008145457A1 (fr) 2008-12-04

Family

ID=39532319

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2008/054807 WO2008145457A1 (fr) 2007-06-01 2008-04-21 Colonne capillaire perfectionnée pour chromatographie en phase gazeuse

Country Status (2)

Country Link
IT (1) ITVE20070034A1 (fr)
WO (1) WO2008145457A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009073270A1 (fr) * 2007-12-03 2009-06-11 Services Petroliers Schlumberger Chromatographie par accélération différentielle
EP2592414A4 (fr) * 2010-07-08 2017-06-28 Daicel Corporation Colonne de séparation/détection et kit associé
CN106908557A (zh) * 2017-03-15 2017-06-30 厦门宜柱科技有限公司 精准可控双相色谱微柱及其制备方法
CN110494746A (zh) * 2017-03-03 2019-11-22 布莱阿姆青年大学 多模态、多检测器液相色谱系统

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60256055A (ja) * 1984-06-01 1985-12-17 Shimadzu Corp 熱分解ガスクロマトグラフ用プレカラム
US5135549A (en) * 1991-01-30 1992-08-04 The Board Of Trustees Of Southern Illinois University Chromatographic technique and apparatus
EP0637748A1 (fr) * 1993-08-02 1995-02-08 Hewlett-Packard Company Chromatographie multi-dimensionelle avec corrélation
WO2002097426A1 (fr) * 2001-05-30 2002-12-05 Katholieke Universiteit Nijmegen Methode permettant d'analyser un echantillon par chromatographie en phase gazeuse sur colonne capillaire et dispositif destine a etre utilise dans cette methode
US20060186029A1 (en) * 2003-07-14 2006-08-24 Waters Investments Limited Separation device with integral guard column
US20060243651A1 (en) * 2005-05-02 2006-11-02 Ricker Robert D Multi-velocity fluid channels in analytical instruments

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60256055A (ja) * 1984-06-01 1985-12-17 Shimadzu Corp 熱分解ガスクロマトグラフ用プレカラム
US5135549A (en) * 1991-01-30 1992-08-04 The Board Of Trustees Of Southern Illinois University Chromatographic technique and apparatus
EP0637748A1 (fr) * 1993-08-02 1995-02-08 Hewlett-Packard Company Chromatographie multi-dimensionelle avec corrélation
WO2002097426A1 (fr) * 2001-05-30 2002-12-05 Katholieke Universiteit Nijmegen Methode permettant d'analyser un echantillon par chromatographie en phase gazeuse sur colonne capillaire et dispositif destine a etre utilise dans cette methode
US20060186029A1 (en) * 2003-07-14 2006-08-24 Waters Investments Limited Separation device with integral guard column
US20060243651A1 (en) * 2005-05-02 2006-11-02 Ricker Robert D Multi-velocity fluid channels in analytical instruments

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009073270A1 (fr) * 2007-12-03 2009-06-11 Services Petroliers Schlumberger Chromatographie par accélération différentielle
US8512457B2 (en) 2007-12-03 2013-08-20 Schlumberger Technology Corporation Differential acceleration chromatography
US8778059B2 (en) 2007-12-03 2014-07-15 Schlumberger Technology Corporation Differential acceleration chromatography
EP2592414A4 (fr) * 2010-07-08 2017-06-28 Daicel Corporation Colonne de séparation/détection et kit associé
CN110494746A (zh) * 2017-03-03 2019-11-22 布莱阿姆青年大学 多模态、多检测器液相色谱系统
EP3589944A4 (fr) * 2017-03-03 2020-12-30 Brigham Young University Système chromatographique liquide multidétecteur multimodal
CN106908557A (zh) * 2017-03-15 2017-06-30 厦门宜柱科技有限公司 精准可控双相色谱微柱及其制备方法

Also Published As

Publication number Publication date
ITVE20070034A1 (it) 2008-12-02

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