WO2008133164A1 - アミノ末端1箇所で配向制御固定化された固定化タンパク質 - Google Patents

アミノ末端1箇所で配向制御固定化された固定化タンパク質 Download PDF

Info

Publication number
WO2008133164A1
WO2008133164A1 PCT/JP2008/057481 JP2008057481W WO2008133164A1 WO 2008133164 A1 WO2008133164 A1 WO 2008133164A1 JP 2008057481 W JP2008057481 W JP 2008057481W WO 2008133164 A1 WO2008133164 A1 WO 2008133164A1
Authority
WO
WIPO (PCT)
Prior art keywords
immobilized
protein
sequence
amino terminal
lysine
Prior art date
Application number
PCT/JP2008/057481
Other languages
English (en)
French (fr)
Inventor
Masahiro Iwakura
Kiyonori Hirota
Hiroyuki Sota
Gou Sarara
Hisashi Takahashi
Yukiko Aruga
Chiori Yamane
Original Assignee
National Institute Of Advanced Industrial Science And Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Institute Of Advanced Industrial Science And Technology filed Critical National Institute Of Advanced Industrial Science And Technology
Priority to US12/596,600 priority Critical patent/US20100130721A1/en
Publication of WO2008133164A1 publication Critical patent/WO2008133164A1/ja

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)

Abstract

　本発明は、アミノ末端1箇所で配向制御固定化された固定化タンパク質であって、 一般式　S1-R1-R2で表されるリジン及びシステイン残基を全く含まないアミノ酸配列［式中、配列は、アミノ末端側からカルボキシ末端側に向かう配列を示し、 S1部分の配列は存在しなくてもよく、存在する場合はリジン及びシステイン残基以外のアミノ酸残基により構成されるスペーサー配列であり； R1部分の配列は、固定化対象タンパク質の配列であり、リジン残基及びシステイン残基を含まないことを特徴とする配列であり； R2部分の配列は存在しなくてもよく、存在する場合はリジン及びシステイン残基以外のアミノ酸残基により構成されるスペーサー配列であることを特徴とする］を有するタンパク質に唯一存在するα－アミノ基を介してタンパク質のアミノ末端１箇所が固定化担体と結合していることを特徴とする固定化タンパク質を提供する。
PCT/JP2008/057481 2007-04-20 2008-04-17 アミノ末端1箇所で配向制御固定化された固定化タンパク質 WO2008133164A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US12/596,600 US20100130721A1 (en) 2007-04-20 2008-04-17 Immobilized protein that is immobilized only at its amino terminus in orientation-controlled manner

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-112218 2007-04-20
JP2007112218A JP2008266221A (ja) 2007-04-20 2007-04-20 アミノ末端１箇所で配向制御固定化された固定化タンパク質

Publications (1)

Publication Number Publication Date
WO2008133164A1 true WO2008133164A1 (ja) 2008-11-06

Family

ID=39925620

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/057481 WO2008133164A1 (ja) 2007-04-20 2008-04-17 アミノ末端1箇所で配向制御固定化された固定化タンパク質

Country Status (3)

Country Link
US (1) US20100130721A1 (ja)
JP (1) JP2008266221A (ja)
WO (1) WO2008133164A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102993276A (zh) * 2012-11-06 2013-03-27 北京博奥森生物技术有限公司 一种具有免疫活性的多肽衍生物及其内参抗体的制备方法和应用
JP2013256484A (ja) * 2012-06-14 2013-12-26 National Institute Of Advanced Industrial Science & Technology 抗体認識結合タンパク質

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8592555B2 (en) * 2008-08-11 2013-11-26 Emd Millipore Corporation Immunoglobulin-binding proteins with improved specificity
JP5339278B2 (ja) * 2008-11-28 2013-11-13 独立行政法人産業技術総合研究所 モノリスゲルを用いたタンパク質アレイ用検出および解析システム
SG162687A1 (en) 2008-12-24 2010-07-29 Millipore Corp Caustic stable chromatography ligands
JP5392682B2 (ja) * 2009-12-22 2014-01-22 独立行政法人産業技術総合研究所 固定化タンパク質
JP5392683B2 (ja) * 2009-12-22 2014-01-22 独立行政法人産業技術総合研究所 固定化タンパク質作製用活性化担体
JP5392684B2 (ja) * 2009-12-22 2014-01-22 独立行政法人産業技術総合研究所 固定化タンパク質の製造方法
JP5769124B2 (ja) * 2010-06-30 2015-08-26 株式会社 京都モノテック 固定化タンパク質および固定化タンパク質作製用活性化担体
SG10201604554WA (en) 2011-06-08 2016-07-28 Emd Millipore Corp Chromatography matrices including novel staphylococcus aureus protein a based ligands
JP5963248B2 (ja) 2012-06-14 2016-08-03 国立研究開発法人産業技術総合研究所 抗体精製用担体並びにその製造方法及びその用途
JP6174361B2 (ja) * 2013-04-19 2017-08-02 株式会社日立製作所 タンパク質固定化担体の製造方法
JP6456831B2 (ja) * 2013-09-04 2019-01-23 プロテノバ株式会社 イムノグロブリン結合ドメイン多量体
JP6950957B2 (ja) 2015-10-22 2021-10-13 プロテノバ株式会社 イムノグロブリン結合ポリペプチド
US20200369716A1 (en) * 2017-08-01 2020-11-26 Carnegie Mellon University Solid-phase synthesis of protein-polymer conjugates on immobilization supports
US11472894B2 (en) 2018-07-23 2022-10-18 Carnegie Mellon University Enzyme-assisted ATRP procedures
US12053529B2 (en) 2018-08-01 2024-08-06 Carnegie Mellon University Amino-reactive positively charged ATRP initiators that maintain their positive charge during synthesis of biomacro-initiators

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63196857A (ja) * 1987-02-10 1988-08-15 Seiko Instr & Electronics Ltd タンパク質もしくはペプチドのアミノ酸配列決定方法
JP2003344396A (ja) * 2002-05-23 2003-12-03 National Institute Of Advanced Industrial & Technology 配向制御したタンパク質の固定化方法およびそれを利用したタンパク質の整列固定化方法
WO2006059737A1 (en) * 2004-11-30 2006-06-08 Semiconductor Energy Laboratory Co., Ltd. Display device and driving method thereof, semiconductor device, and electronic apparatus
WO2008044692A1 (fr) * 2006-10-10 2008-04-17 National Institute Of Advanced Industrial Science And Technology Protéine adaptée pour une commande d'orientation/immobilisation de protéine et support d'immobilisation destiné à la protéine

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63196857A (ja) * 1987-02-10 1988-08-15 Seiko Instr & Electronics Ltd タンパク質もしくはペプチドのアミノ酸配列決定方法
JP2003344396A (ja) * 2002-05-23 2003-12-03 National Institute Of Advanced Industrial & Technology 配向制御したタンパク質の固定化方法およびそれを利用したタンパク質の整列固定化方法
WO2006059737A1 (en) * 2004-11-30 2006-06-08 Semiconductor Energy Laboratory Co., Ltd. Display device and driving method thereof, semiconductor device, and electronic apparatus
WO2008044692A1 (fr) * 2006-10-10 2008-04-17 National Institute Of Advanced Industrial Science And Technology Protéine adaptée pour une commande d'orientation/immobilisation de protéine et support d'immobilisation destiné à la protéine

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
IWAKURA M. ET AL.: "Evolutional design of a hyperactive cysteine- and methionine-free mutant of Escherichia coli dihydrofolate reductase", J. BIOL. CHEM., vol. 281, no. 19, 2006, pages 13234 - 13246, XP003022197 *
TAKENAWA T. ET AL.: "Cyanocysteine-mediated molecular dissection of dihydrofolate reductase: occurrence of intra- and inter-molecular reactions forming a peptide bond", J. BIOCHEM., vol. 123, no. 6, 1998, pages 1137 - 1144, XP009018697 *
YAMAMOTO Y. ET AL.: "Site-specific PEGylation of a lysine-deficient TNF-alpha with full bioactivity", NAT. BIOTECH., vol. 21, no. 5, 2003, pages 546 - 552, XP003007344 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013256484A (ja) * 2012-06-14 2013-12-26 National Institute Of Advanced Industrial Science & Technology 抗体認識結合タンパク質
CN102993276A (zh) * 2012-11-06 2013-03-27 北京博奥森生物技术有限公司 一种具有免疫活性的多肽衍生物及其内参抗体的制备方法和应用
CN102993276B (zh) * 2012-11-06 2014-12-10 北京博奥森生物技术有限公司 一种具有免疫活性的多肽衍生物及其内参抗体的制备方法和应用

Also Published As

Publication number Publication date
US20100130721A1 (en) 2010-05-27
JP2008266221A (ja) 2008-11-06

Similar Documents

Publication Publication Date Title
WO2008133164A1 (ja) アミノ末端1箇所で配向制御固定化された固定化タンパク質
WO2007148224A8 (en) Polypeptide
WO2009137721A3 (en) Lysosomal targeting peptides and uses thereof
AU2009209251A8 (en) Stabilized Angiopoietin-2 antibodies and uses thereof
WO2010115835A3 (en) Stabilizing composition for immobilized biomolecules
WO2010089171A3 (de) Verfahren zur aminierung von polycyclischen verbindungen unter verwendung von transaminasen
EP1992639A4 (en) PEPTIDE VACCINE FOR INDUCING THE PRODUCTION OF AMYLOID ANTI-PEPTIDE ANTIBODIES
WO2006131928A8 (en) Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis
NZ584893A (en) PORCINE DC-SIGN, ICAM-3 AND LSECtin AND USES THEREOF
NZ597692A (en) Anti-IGF antibodies
WO2009113828A3 (en) Peptide nucleic acid derivatives with good cell penetration and strong affinity for nucleic acid
NZ595303A (en) Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides
MX2007007030A (es) Enzimas para procesamiento de almidon.
WO2010106180A3 (en) Optimized fc variants
WO2009079212A3 (en) Linked peptide fluorogenic biosensors
WO2007059312A3 (en) Methods and compositions comprising non-natural amino acids
WO2007041689A3 (en) Methods of site-specific labeling of molecules and molecules produced thereby
WO2006116181A3 (en) Regulatory t cell mediator proteins and uses thereof
EA201170879A1 (ru) Полипептиды с ксиланазной активностью
WO2008066816A3 (en) Derivatization or ligaton of peptides
WO2009083225A3 (en) Antibody-containing formulation for the use for treating cardiovascular diseases associated with atherosclerosis
WO2008045015A8 (en) Imino and amino sugar purification
WO2012085279A3 (en) Method for cross-linking peptides
DE602007004110D1 (de) Hausstaubmilbenallergen
WO2012175924A3 (en) Incorporation of substituted lysines into polypeptides

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08740551

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 12596600

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 08740551

Country of ref document: EP

Kind code of ref document: A1