WO2007041689A3 - Methods of site-specific labeling of molecules and molecules produced thereby - Google Patents

Methods of site-specific labeling of molecules and molecules produced thereby Download PDF

Info

Publication number
WO2007041689A3
WO2007041689A3 PCT/US2006/039061 US2006039061W WO2007041689A3 WO 2007041689 A3 WO2007041689 A3 WO 2007041689A3 US 2006039061 W US2006039061 W US 2006039061W WO 2007041689 A3 WO2007041689 A3 WO 2007041689A3
Authority
WO
WIPO (PCT)
Prior art keywords
molecules
site
methods
specific labeling
produced
Prior art date
Application number
PCT/US2006/039061
Other languages
French (fr)
Other versions
WO2007041689A2 (en
Inventor
Christopher T Walsh
Jun Yin
Paul D Straight
Roberto Kolter
Zhe Zhou
Original Assignee
Harvard College
Christopher T Walsh
Jun Yin
Paul D Straight
Roberto Kolter
Zhe Zhou
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College, Christopher T Walsh, Jun Yin, Paul D Straight, Roberto Kolter, Zhe Zhou filed Critical Harvard College
Publication of WO2007041689A2 publication Critical patent/WO2007041689A2/en
Publication of WO2007041689A3 publication Critical patent/WO2007041689A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention relates to methods of site-specific labeling of molecules and molecules produced thereby. The methods described herein are based, at least in part, on the discovery of novel peptide substrates for phosphopantetheinyl transferases, which can be used for site-specific labeling of both proteinaceous and non-proteinaceous molecules using a phosphopantetheinylation reaction.
PCT/US2006/039061 2005-10-04 2006-10-04 Methods of site-specific labeling of molecules and molecules produced thereby WO2007041689A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US72364005P 2005-10-04 2005-10-04
US60/723,640 2005-10-04

Publications (2)

Publication Number Publication Date
WO2007041689A2 WO2007041689A2 (en) 2007-04-12
WO2007041689A3 true WO2007041689A3 (en) 2007-10-04

Family

ID=37906872

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/039061 WO2007041689A2 (en) 2005-10-04 2006-10-04 Methods of site-specific labeling of molecules and molecules produced thereby

Country Status (1)

Country Link
WO (1) WO2007041689A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12031177B1 (en) 2021-06-03 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009092808A1 (en) * 2008-01-24 2009-07-30 National University Of Ireland, Maynooth Method for detecting phosphopantetheinyl transferase activity
ES2324192B1 (en) * 2008-01-30 2010-06-17 PUIG BEAUTY & FASHION GROUP, S.L. PEPTIDIC DERIVATIVES USEFUL IN THE TREATMENT, CARE OR CLEANING OF THE SKIN, MUCOSA, LEATHER HAIR OR NAILS.
WO2010127186A1 (en) 2009-04-30 2010-11-04 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
PL2556171T3 (en) 2010-04-05 2016-04-29 Prognosys Biosciences Inc Spatially encoded biological assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
WO2012139110A2 (en) 2011-04-08 2012-10-11 Prognosys Biosciences, Inc. Peptide constructs and assay systems
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP2855520B1 (en) * 2012-06-04 2018-09-26 Novartis AG Site-specific labeling methods and molecules produced thereby
CN111233978A (en) 2013-03-15 2020-06-05 普罗格诺西斯生物科学公司 Methods for detecting peptide/MHC/TCR binding
US9868979B2 (en) 2013-06-25 2018-01-16 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
US10288608B2 (en) 2013-11-08 2019-05-14 Prognosys Biosciences, Inc. Polynucleotide conjugates and methods for analyte detection
ES2955916T3 (en) 2015-04-10 2023-12-11 Spatial Transcriptomics Ab Multiplex analysis of biological specimens of spatially distinguished nucleic acids
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
EP3894592A2 (en) 2018-12-10 2021-10-20 10X Genomics, Inc. Generating spatial arrays with gradients
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
CN109750055B (en) * 2019-03-13 2022-12-23 南开大学 Method for improving protein nucleic acid biological coupling efficiency based on alpha helical handle
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
EP3891300B1 (en) 2019-12-23 2023-03-29 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
ES2965354T3 (en) 2020-04-22 2024-04-12 10X Genomics Inc Methods for spatial analysis using targeted RNA deletion
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
EP4162074B1 (en) 2020-06-08 2024-04-24 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4165207A1 (en) 2020-06-10 2023-04-19 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
CN116034166A (en) 2020-06-25 2023-04-28 10X基因组学有限公司 Spatial analysis of DNA methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005090388A1 (en) * 2004-03-19 2005-09-29 The University Of Queensland Alpha helical mimics, their uses and methods for their production

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005090388A1 (en) * 2004-03-19 2005-09-29 The University Of Queensland Alpha helical mimics, their uses and methods for their production

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
GRIFFIN ET AL: "Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 281, 10 July 1998 (1998-07-10), pages 269 - 272, XP002116989, ISSN: 0036-8075 *
HUANG Y-T ET AL: "Backbone dynamics of Escherichia coli thioesterase/protease I: evidence of a flexible active-site environment for a serine protease", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 307, no. 4, 6 April 2001 (2001-04-06), pages 1075 - 1090, XP004461709, ISSN: 0022-2836 *
PUYAL C ET AL: "DESIGN OF A SHORT MEMBRANE-DESTABILIZING PEPTIDE COVALENTLY BOUND TO LIPOSOMES", BIOCHIMICA ET BIOPHYSICA ACTA. BIOMEMBRANES, AMSTERDAM, NL, vol. 1195, 1994, pages 259 - 266, XP002918790, ISSN: 0005-2736 *
WATANABE K ET AL: "Crystal Structure of an Acyl-ACP Dehydrogenase from the FK520 Polyketide Biosynthetic Pathway: Insights into Extender Unit Biosynthesis", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 334, no. 3, 28 November 2003 (2003-11-28), pages 435 - 444, XP004472513, ISSN: 0022-2836 *
YIN J, STRAIGHT P D, MACLOUGHLIN S M, ZHOU Z, LIN A, GOLAN D E, KELLEHER N L, KOLTER R, WALSH C: "Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 102, no. 44, November 2005 (2005-11-01), pages 15815 - 15820, XP002441313, ISSN: 0027-8424 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12031177B1 (en) 2021-06-03 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts

Also Published As

Publication number Publication date
WO2007041689A2 (en) 2007-04-12

Similar Documents

Publication Publication Date Title
WO2007041689A3 (en) Methods of site-specific labeling of molecules and molecules produced thereby
WO2006131928A3 (en) Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis
WO2009105670A3 (en) Substrates for multiplexed assays and uses thereof
AU2008257801A8 (en) RSV-specific binding molecules and means for producing them
WO2007075891A3 (en) Multiplex assays using magnetic and non-magnetic particles
MX2009006199A (en) Abeta antibody parenteral formulation.
WO2007008583A3 (en) Improved protein expression comparison assay results and applications
WO2007039903A3 (en) Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis
WO2006028936A3 (en) Heteromultimeric molecules
WO2007100506A3 (en) Mass tags for quantitative analyses
WO2007058527A3 (en) Inducible expression systems
WO2006130374A3 (en) Tweak binding antibodies
WO2008066816A3 (en) Derivatization or ligaton of peptides
WO2005050224A3 (en) Small molecule and peptide arrays and uses thereof
WO2006135793A3 (en) Protein engineering with analogous contact environments
WO2007107516A3 (en) D-amino acid selection for soybean
WO2007001962A3 (en) Systems and methods for generating biological material
WO2005017205A3 (en) Nucleic acid mapping using linear analysis
WO2005116252A8 (en) Methods for evaluating ribonucleotide sequences
WO2007008072A3 (en) CROSS-ß STRUCTURE BINDING COMPOUNDS
WO2007067433A3 (en) Poly(trimethylene terephthalate)/poly(alpha-hydroxy acid) films
WO2006074334A3 (en) Identification of rna targets using helicases
WO2009079212A3 (en) Linked peptide fluorogenic biosensors
WO2008131002A3 (en) Generation of acyl amino acids
WO2008011433A3 (en) Styrenated terpene resin as well as methods of making and using the same

Legal Events

Date Code Title Description
NENP Non-entry into the national phase

Ref country code: DE

32PN Ep: public notification in the ep bulletin as address of the adressee cannot be established

Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 112(1) EPC, EPO FORM 1205A OF 19.06.2008

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 06825529

Country of ref document: EP

Kind code of ref document: A2

122 Ep: pct application non-entry in european phase

Ref document number: 06825529

Country of ref document: EP

Kind code of ref document: A2