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Definitions

• the present invention relates to novel mercaptopurine derivatives and to pharmaceutical compositions thereof.
• the mercaptopurine derivatives are useful for treatment of various diseases or disorders, in particular, proliferative, inflammatory, skin and autoimmune diseases or disorders.
• 6-Mercaptopurine (6-MP) first synthesized by Elion et at. (1952), as well as metabolically related compounds thereof such as azathioprine, 6-mercaptopurine riboside (6-MPR, also known as thioinosine), 6-thiouric acid, 6- methylmercaptopurine (6-MMP), 6-methyl-thioinosine 5 '-monophosphate, and 6- thioguanine (6-TG), are structural analogs of adenine and guanine, thus the therapeutic and, in particular, antiproliferative properties of this class of compounds have long been recognized.
• 6-MPR 6-mercaptopurine riboside
• 6-thiouric acid 6- methylmercaptopurine
• 6-MMP 6-methyl-thioinosine 5 '-monophosphate
• 6- thioguanine 6-thioguanine
• 6-MP and 6-MPR are cytotoxic prodrugs that interfere with nucleic acid synthesis by either direct substitution of deoxythioGTP, thereby causing further modifications and mismatches upon replication, or by inhibition of de novo purine biosynthesis (Karran, 2006).
• 6-MP, azathioprine and 6-MPR are the most prominent therapeutic agents, and as such are currently used in the treatment of a variety of medical conditions such as cancer (in particular leukemia), inflammatory bowel diseases (in particular Crohn's disease and ulcerative colitis), psoriatic arthritis, psoriasis, Reiter's syndrome, Behcet's disease, polymyositis, systemic lupus erythematosus and systemic vasculitis.
• Azathioprine is further used in the prevention of rejection following organ transplants (Carroll et al, 2003; Watters and McLeod, 2003; Dubinsky, 2004).
• Thiopurines are prodrugs that are transformed enzymatically by three competitive enzymatic pathways: the first, xanthine oxidase, catalyzes the oxidation of 6-MP to the biologically inactive metabolite, thiouric acid; the second, hypoxanthine-guanine phosphoribosyl transferase (HGPRT), catalyzes the formation of 6-thioinosine monophosphate that may further be converted by cellular enzymes to thioguanine nucleotides that may then be incorporated by polymerase directly into the DNA; and the third, thiopurine methy ⁇ transferase (TPMT), catalyzes S-methylation of 6-MP and 6-thioinosine monophosphate to 6-methyl- mercaptopurine (MeMP) and S-methyl-thioinosine 5 '-monophosphate (MeTIMP), respectively.
• xanthine oxidase catalyzes the
• the latter is a potent inhibitor of phosphoribosylpyrophosphate amidotransferase, the first step of de novo purine biosynthesis, thereby causing purine depletion (Karran, 2006; Coulthard and Hogarth, 2005; Krynetski and Evans, 1999; Cara, et a/., 2004).
• 6-MP, 6-MPR and various derivatives thereof have exceptional therapeutic potential, particularly in the fields of proliferative and inflammatory diseases and disorders, their practice is often limited, mainly due to cytotoxicity and/or poor pharmacokinetic thereof, as described hereinabove.
• novel therapeutic agents which would possess the biological activities of mercaptopurines, and at the same time would circumvent the limitations associated with the use of these compounds in various treatments.
• mercaptopurine derivatives e.g., S-allylthio-6-mercaptopurine and S-allylthio-6- mercaptopurine 9-riboside
• S-allylthio-6-mercaptopurine and S-allylthio-6- mercaptopurine 9-riboside are highly efficient anti-proliferative agents, having DNA synthesis inhibitory effect that is either equal or superior to that of 6- mercaptopurine and 6-mercaptopurine riboside, respectively, while further circumventing the limitations associated with the administration of each of the latter alone.
• the present invention thus relates to a compound of the general formula
• R] to R 3 each independently is either a covalent bond or selected from H, halogen, SH, NRsR 6 , O-hydrocarbyl, S-hydrocarbyl, heteroaryl, unsubstituted hydrocarbyl, hydrocarbyl substituted by halogen, CN, SCN, NO 25 OR 5 , SR 5 , NR 5 R 6 or heteroaryl, or a carbohydrate residue, wherein R 5 and R 6 each independently is H or hydrocarbyl or R 5 and R 6 together with the nitrogen atom to which they are attached form a 5- or 6-membered saturated heterocyclic ring optionally containing 1-2 further heteroatoms selected from the group consisting of oxygen, nitrogen and sulfur, the additional nitrogen being unsubstituted or substituted by alkyl substituted by halogen, hydroxy 1 or phenyl, provided that one Of R 1 , R 2 and R 3 is a covalent bond;
• R 4 is H, alkyl, a carbohydrate residue or NR 5 R 6 , wherein R 5 and R 6 each independently is H or hydrocarbyl or R 5 and R 6 together with the nitrogen atom to which they are attached form a 5- or 6-membered saturated heterocyclic ring optionally containing 1-2 further heteroatoms selected from the group consisting of oxygen, nitrogen and sulfur, the additional nitrogen being unsubstituted or substituted by alkyl substituted by halogen, hydroxyl or phenyl; Y is heteroaryl, unsubstituted hydrocarbyl, or hydrocarbyl substituted by halogen, CN, SCN, NO 2 , OR 7 , SR 7 , NR 7 R 8 or heteroaryl, wherein R 7 and R 8 each independently is H or hydrocarbyl or R 7 and R 8 together with the nitrogen atom to which they are attached form a 5- or 6-membered saturated heterocyclic ring optionally containing 1-2 further heteroatoms selected from the group consisting of oxygen, nitrogen
• the present invention relates to a pharmaceutical composition
• a pharmaceutical composition comprising a compound as defined above, or a pharmaceutically acceptable salt thereof, and a pharmaceutical acceptable carrier.
• the compounds and the pharmaceutical compositions of the present invention may be used for treatment of a disease or disorder selected from a proliferative disease or disorder, an inflammatory disease or disorder, a skin disease or disorder, or an immune disease or disorder.
• the present invention relates to use of a compound as defined above, or a pharmaceutically acceptable salt thereof, for the treatment of a disease or disorder selected from a proliferative disease or disorder, an inflammatory disease or disorder, a skin disease or disorder, or an immune disease or disorder.
• the present invention further provides a method for treatment of a disease or disorder selected from a proliferative disease or disorder, an inflammatory disease or disorder, a skin disease or disorder, or an immune disease or disorder, said method comprising administering to an individual in need a therapeutically effective amount of a compound as defined above, or a pharmaceutically acceptable salt thereof.
• Figs. 1A-1B show spectrum analysis of S-allylthio-6-mercaptopurine
• SA-6MP (hereinafter SA-6MP) (IA) and S-allylthio-6-mercaptopurine 9-riboside (hereinafter
• Figs. 2A-2C show cell proliferation of Daudi cells (2A), HeIa cells (2B) and
• N87 cell (2C) treated with different concentrations (0-200 ⁇ M of either 6MP or SA-
• Figs. 3A-3D show the lethal effect of 6-MP and SA-6MP on MDR HT-29 cells (3A), HeIa cells (3B), Daudi cells (3C) and B-CLL cells (3D).
• Cells were incubated with the prodrugs for 16 h at 37 0 C and stained with trypan blue and propidium iodine (PI). Cells were counted after the trypan blue exclusion test and the percentage of viable cells was calculated. Alternatively, the percentage of viable PI stained cells was determined by FACS analysis. The values presented are the means ⁇ SD. Viability values that are significantly different (p ⁇ 0.05) from non- treated cells (*).
• Fig. 4 shows cell death assessment using PI staining.
• MDR HT-29 cells were cultured in the presence of either 6-MP or SA-6MP (150 ⁇ M) for 16 h at 37 0 C and stained with propidium iodide (PI). Images of treated-cells were observed by phase- contrast microscopy to determine normal growth patterns (T, upper panel) and fluorescent images of the treated-cells indicated dead cells stained with PI, (F, lower panel).
• Figs. 5A-5F show Fluorescence Activated Cell Sorting (FACS) analysis of chronic lymphocytic leukemia B-cells (B-CLL) treated for 16 h at 37 0 C with 50 ⁇ M of 6MP (5B), 100 ⁇ M of 6MP (5C) 5 150 ⁇ M of 6MP (5D) 3 50 ⁇ M of SA-6MP (5E) or 100 ⁇ M of SA-6MP (5F) VJ. untreated cells (5A). Cells were stained with Annexin-Cy5 and analyzed by FACS. The percentage of apoptotic cells at various drug concentrations are superimposed in the upper right part of each analysis. Figs.
• FACS Fluorescence Activated Cell Sorting
• 6A-6B show percentage of apoptotic cells (6A) and of dead cells (6B) in chronic lymphocytic leukemia B-cells (B-CLL) treated with different concentrations (0-150 ⁇ M) of 6-MP or SA-6MP for 16 h at 37 0 C. Apoptotic cells were measured using FACS analysis with annexin and cell death was measured using trypan blue test.
• Figs. 7A-7B show the effects of 6-MP 5 6-MPR 5 SA-6MP and SA-6MPR on cell proliferation in Daudi (7A) and N87 (7B) cell lines. Cells were incubated with different concentrations (0-150 ⁇ M) of the prodrugs for 16h at 37 0 C, and the antiproliferative effect was assessed using the XTT assay. The values presented are the means ⁇ SEM.
• the present invention relates, in one aspect, to a mercaptopurine derivatives of the general formula X-S-S-Y, as defined hereinabove.
• mercaptop urines have been shown heretofore to exert beneficial therapeutic activity, particularly as antiproliferative agents.
• These mercaptopurines share common structural features such as having a purine-like skeleton and one or more free thiol groups attached thereto, e.g., to the carbon atoms in the skeleton.
• purine-like skeleton refers to a structure composed of a pyrimidine ring and an imidazole ring fused to one another, as well as to analogs thereof.
• purine-like analogs include structures in which the pyrimidine ring is replaced by a pirazine, a pyridine or a phenyl ring and/or the imidazole ring is replaced by a furan ring, a pyrrole ring and the like.
• analog refers to a compound which shares chemical and/or structural characteristics of a mercaptopurine and is thus capable, for example, of blocking nucleic acid synthesis in the body.
• derivative describes a compound which has been subjected to a chemical modification while maintaining its main structural features. Such chemical modifications can include, for example, replacement of one or more substituents and/or one or more functional moieties.
• halogen includes fluoro, chloro, bromo, and iodo, and it is preferably chloro.
• hydrocarbyl in any of the definitions of the different radicals Ri to R 8 refers to a radical containing only carbon and hydrogen atoms that may be saturated or unsaturated, linear or branched, cyclic or acyclic, or aromatic, and includes C 1 -C 2O alkyl, C 2 -C 20 alkenyl, C 2 -C 20 alkynyl, C 3 -C 20 cycloalkyl, C 3 -C 20 cycloalkenyl, C 6 -C 14 aryl, (Ci-C 20 )alkyl(C 6 -Ci 4 )aryl, and (C 6 -C 14 ) aryl(C r C 20 )alkyl.
• C 1 -C 20 alkyl typically means a straight or branched hydrocarbon radical having 1-20 carbon atoms and includes, for example, methyl, ethyl, n- propyl, isopropyl, n-butyl, sec-butyl, isobutyl, tert-butyl, n-pentyl, 2,2- dimethylpropyl, n-hexyl, n-heptyl, n-octyl, and the like.
• C 2 -C 20 alkenyl and “C 2 -C 20 alkynyl” typically mean straight and branched hydrocarbon radicals having 2-20 carbon atoms and 1 double or triple bond, respectively, and include ethenyl, propenyl, 3-buten-l-yl, 2-ethenylbutyl, 3-octen-l-yl, and the like, and propynyl, 2- butyn-1-yl, 3-pentyn-l-yl, and the like.
• C 2 -C 6 alkenyl radicals are preferred.
• C 3 -C 20 cycloalkyl means a cyclic or bicyclic hydrocarbyl group such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, adamantyl, bicyclo[3.2.1]octyl, bicyclo[2.2.1]heptyl, and the like.
• C 6 -C 14 aryl denotes a carbocyclic aromatic radical such as phenyl and naphthyl and the term "ar(Ci-C 20 )alkyl” denotes an arylalkyl radical such as benzyl andphenetyl.
• each one of said hydrocarbyls is preferably a C 1 -C 6 alkyl, most preferably methyl or ethyl, or an aryl, most preferably phenyl, or an aralkyl, most preferably benzyl, radical.
• each one of said hydrocarbyls is preferably a C 2 -C 8 alkenyl or alkynyl, more preferably C 2 -CO alkenyl or alkynyl, most preferably allyl or propargyl, radical.
• R 5 to R 8 each independently is H or hydrocarbyl as defined above or form together with the N atom to which they are attached a saturated, preferably a 5- or 6-membered, heterocyclic ring, optionally containing 1 or 2 further heteroatoms selected from nitrogen, oxygen, and sulfur.
• Such rings may be substituted, for example with one or two CpCe alkyl groups, or with one alkyl or hydroxyalkyl group at a second nitrogen atom of the ring, for example in a piperazine ring.
• radicals NR 5 R 6 and NR 7 R 8 include, without being limited to, amino, dimethylamino, diethylamino, ethylmethylamino, phenylmethylamino, pyrrolidino, piperidino, tetrahydropyridino, piperazino, ethylpiperazino, hydroxyethylpiperazino, morpholino, thiomorpholino, thiazolino, and the like.
• heteroaryl refers to a radical derived from a mono- or poly-cyclic ring containing one to three heteroatoms selected from the group consisting of N, O and S, with unsaturation of aromatic character.
• Non-limiting examples of heteroaryl include pyrrolyl, furyl, thienyl, pyrazolyl, imidazolyl, oxazolyl, isoxazolyl thiazolyl, isothiazolyl, pyridyl, 1,3-benzodioxinyl, pyrazinyl, pyrimidinyl, 1,3,4-triazinyl,
• the heteroaryl ring may be substituted. It is to be understood that when a polycyclic heteroaromatic ring is substituted, the substitution may be in the heteroring or in the carbocyclic ring.
• mercaptopurine ribosides being analogous to the ribonucleotide building blocks of RNA 5 can block nucleic acid synthesis in the body.
• at least one OfR 1 -R 4 is a carbohydrate residue.
• carbohydrate describes a molecule containing carbon, hydrogen and oxygen atoms.
• the carbohydrate can be cyclic or linear, saturated or unsaturated and substituted or unsubstituted.
• the carbohydrate residue comprises one or more saccharide residues.
• saccharide residue as used herein encompasses any residue of a sugar moiety, including monosaccharides, oligosaccharides and polysaccharides.
• the saccharide can be a saccharide derivative such as, but not limited to, glucosides, ethers, esters, acids and amino saccharides.
• Monosaccharides consist of a single sugar molecule which cannot be further decomposed by hydrolysis.
• Examples of monosaccharides include, without limitation, pentoses such as, but not limited to, arabinose, xylose and ribose.
• Oligosaccharides are chains composed of saccharide units. As commonly defined in the art and herein, oligosaccarides are composed of up to nine saccharide units. Examples of oligosaccharides include, without limitation, disaccharides such as, but not limited to, sucrose, maltose, lactose and cellobiose; trisaccharides such as, but not limited to, mannotriose, raffinose and melezitose; and tetrasaccharides such as amylopectin, Syalyl Lewis X (SiaLex) and the like.
• disaccharides such as, but not limited to, sucrose, maltose, lactose and cellobiose
• trisaccharides such as, but not limited to, mannotriose, raffinose and melezitose
• tetrasaccharides such as amylopectin, Syalyl Lewis X (SiaLex) and the
• polysaccharide refers to compounds composed of at least 10 saccharide units and up to hundreds and even thousands of monosaccharide units per molecule, which are held together by glycoside bonds and range in their molecular weights from around 5,000 and up to millions of Daltons.
• Examples of common polysaccharides include, but are not limited to, starch, glycogen, cellulose, gum arabic, agar and chitin.
• the carbohydrate residue is a saccharide residue, preferably a monosaccharide residue, more preferably, a 5- or 6-membered monosaccharide residue, most preferably a riboside residue.
• R 4 is a riboside residue, such that the purine residue according to the present invention has a structure similar to a purine ribonucleotide, i.e. the building block of RNA, and hence can interfere with RNA synthesis and exhibit anti- proliferative effect.
• the purine residue according to the present invention may be linked via a disulfide bond to Y as defined above at various positions of the pyrimidine ring, namely at positions 2, 6 or 8.
• the purine residue is linked via a disulfide bond to Y at position 6 of the pyrimidine ring, namely R 2 is a covalent bond.
• the purine residue is linked via a disulfide bond to Y at position 2 of the pyrimidine ring, namely Ri is a covalent bond.
• the purine residue is linked via a disulfide bond to Y at position 8 of the mercaptopurine, namely R 3 is a covalent bond.
• R 2 is a covalent bond
• Rj is H, SH, methyl or dimethylamino
• R 3 is H, SH or methyl
• R 4 is H or a 5-membered monosaccharide residue
• Y is allyl or propargyl.
• R 2 is a covalent bond
• Ri and R 3 each is H
• R 4 is H or a riboside residue
• Y is allyl or propargyl.
• R] is a covalent bond
• R 2 is H, SH, methyl or dimethylamino
• R 3 is H, SH or methyl
• R 4 is H or a 5-membered monosaccharide residue
• Y is allyl or propargyl.
• Ri is a covalent bond
• R 2 and R 3 each is H
• R 4 is H or a riboside residue
• Y is allyl or propargyl.
• R 3 is a covalent bond
• Rj and R 2 each independently is H, SH, methyl or dimethylamino
• R 4 is H or a 5-membered monosaccharide residue
• Y is allyl or propargyl.
• R 3 is a covalent bond
• Ri and R 2 each is H
• R 4 is H or a riboside residue
• Y is allyl or propargyl.
• the compounds of the present invention are derived from 6-mercaptopurines and hence are the compounds wherein R 2 is a covalent bond.
• Representative examples of these compounds include, without limitation, the compounds wherein R h R 3 and R 4 each is H, and Y is allyl, namely S-allylthio-6-mercaptopurine, which is also abbreviated herein as SA-6MP; and the compound wherein R 1 and R 3 each is H, R 4 is riboside, and Y is allyl, namely S- allylthio-6-mercaptopurine 9-riboside, which is also abbreviated herein as SA- 6MPR (see Scheme 1 hereinafter).
• Allicin the biologically active compound derived from garlic, is produced upon crushing the garlic clove, thus exposing the enzyme alliinase to its substrate, alliin (S-allyl-L-cysteine sulfoxide) (Stoll and Seebeck, 1951).
• Allicin is known to confer many health beneficial effects, amongst which are its anti microbial, anti fungal and anti parasitic activities (Koch and Lawson, 1996), antihypertensive activity (Elkayam et al, 2000), remedial effects on cardiovascular risk factors (Eilat et al, 1995; Abramovitz et al, 1999; Gonen et al, 2005), anti-inflammatory activity (Lang et al, 2004) and anticancer activity (Koch and Lawson, 1996; Agarwal, 1996; Hirsch et al, 2000; Miron et al, 2003; Arditti et al, 2005).
• Allicin is a short-lived compound, which rapidly reacts with free thiol groups and penetrates biological membranes with ease (Rabinkov et al, 1998, 2000; Miron et al, 2000), thus shows promise in affecting different metabolic pathways (Agarwal, 1996).
• allicin is very unstable, it quickly disintegrates in the blood a few minutes after being administered both in vitro in human blood (Freeman and Kodera, 1995) and in vivo in rats (Lachmann et al, 1994), and therefore, its therapeutic effect is limited to targets close to the gastrointestinal tract.
• allicin derivatives such as allylmercaptoglutathione
• GSSA GSSA
• CSSA S-allylmercaptocysteine
• CPSSA S-allylmercaptocaptopril
• N87 cells a human gastric adenocarcinoma cell line
• CB2 cells a Chinese hamster ovary cell line
• SA-6MP and SA-6MPR have been readily prepared by reacting 6-MP or 6-MPR, respectively, with allicin, in an aqueous solvent, as depicted in Scheme 1 hereinafter.
• the reaction is preferably performed under mild basic conditions, e.g., in the presence of a buffer having a basic pH in the range of 7.2-8.5.
• the process of preparing the compound of the present invention may further comprise isolating the obtained compound from the reaction mixture, and optionally a purification of the obtained compound by any of the purification methods known.
• the isolation of the compound from the reaction mixture was effected by collecting the precipitate formed upon cooling the reaction mixture; and purification of the precipitate was performed by recrystallization from a water: ethanol mixture.
• the compounds of the present invention may further be prepared by any other preparative methods used for the preparation of unsymmetrical disulfides as described by Antoniow and Witt (2007).
• both SA-6MP and SA-6MPR were found to act as highly efficient antiproliferative agents.
• both compounds have been shown to have DNA synthesis inhibitory effect, which was either equal or superior to that of the non-conjugated components, namely, 6-MP, 6-MPR and allicin.
• an increased lethal effect and a significant increase in the percentage of apoptotic B- CLL cells were observed in cells treated with SA-6MP, as compared with cancer cells treated with the non-conjugated mercaptopurine 6-MP.
• the high efficacy of the compounds of the present invention to induce apoptosis in B-CLL cells indicates that these compounds are highly efficient as therapeutic agents for the treatment of leukemia.
• the therapeutic efficacy of these compounds was further reflected by the inhibition of the metabolic activity of various cancer cells, which was found to be about 90% for a range of cancer cells.
• 6-MP-resistant MOLT4 cells exhibited enhanced sensitivity to methylmercaptopurine riboside (meMPR) (Fotoohi et al, 2006), suggesting the existence of a distinct transport route for meMPR and the bypass of that of 6-MP, a possible explanation for the "inverted" sensitivity may dwell in 6-MP resistance mechanisms.
• the resistant cells exhibited significant reduction in levels of mRNA encoding several proteins involved in the de novo purine synthesis, as well as in levels of ribonucleoside triphosphates, as compared to non-resistant cells.
• the allylthio moiety of the new derivative protects the free SH from such oxidation. Only later, upon entry to the cellular reducing environment is the mixed disulfide SA-6MP cleaved, which renders higher efficiency at lower concentrations.
• the proposed mechanism for the reaction of SA-6MP with free thiols in the cell is depicted in Scheme 2 hereinafter. The highest antiproliferative activity of all the compounds tested was exerted by allicin (Miron et al, 2003, 2007; Arditti et al, 2005).
• the compounds of the present invention can be efficiently and beneficially utilized in the treatment of medical conditions that are treatable by mercaptopurines. Furthermore, in cases wherein the compound of the present invention is a purine residue linked via a disulfide bond to an allyl group, the compounds of the present invention can further be utilized in the treatment of medical conditions that are treatable by allicin.
• the present invention thus relates to a pharmaceutical composition
• a pharmaceutical composition comprising a compound as defined above, or a pharmaceutically acceptable salt thereof, and a pharmaceutical acceptable carrier.
• Examples of medical conditions that are currently known as treatable by mercaptopurines include, without being limited to, proliferative diseases and disorders, particularly leukemia, inflammatory diseases and disorders, skin diseases and disorders and immune diseases and disorders.
• Examples of medical conditions that are currently known as treatable by allicin include, without being limited to, cardiovascular diseases and cardiovascular risk factors, hypertension, proliferative diseases and disorders such as cancer, bacterial infections, viral infections, parasitic infections and fungal infections.
• the pharmaceutical composition of the present invention is for treatment of a disease or disorder selected from a proliferative disease or disorder, an inflammatory disease or disorder, a skin disease or disorder, or an immune disease or disorder.
• a disease or disorder selected from a proliferative disease or disorder, an inflammatory disease or disorder, a skin disease or disorder, or an immune disease or disorder.
• Preferred compounds for such uses are compounds wherein R 2 is a covalent bond, Ri and R 3 each is H, R 4 is H or a riboside residue, and Y is allyl, namely SA-6MP or SA-6MPR, respectively, or pharmaceutically acceptable salts thereof.
• proliferative disease or disorder refers to a disease or disorder characterized by enhanced cell proliferation.
• Cell proliferation conditions which may be prevented or treated by the present invention include, for example, malignant tumors such as cancer and benign tumors.
• the proliferative disease or disorder that can be treated with the compounds of the present invention may be cancer such as brain cancers such as glioblastoma multiforme, anaplastic astrocytoma, astrocytoma, ependyoma, oligodendroglioma, medulloblastoma, meningioma, sarcoma, hemangioblastoma, and pineal parenchymal; skin cancers such as melanoma and Kaposi's sarcoma; papilloma, blastoglioma, ovarian cancer, prostate cancer, squamous cell carcinoma, astrocytoma, head cancer, neck cancer, bladder cancer, breast cancer, lung cancer, colorectal cancer, thyroid cancer, pancreatic cancer, gastric cancer, hepatocellular carcinoma, leukemia, lymphoma, Hodgldn's lymphoma and Burkitt's lymphoma.
• brain cancers such as gli
• both SA-6MP and SA-6MPR were found to be highly effective in inhibiting the growth and/or killing cancerous blood cells, thus, these compounds are particularly effective for the treatment of leukemia.
• Other non cancerous proliferative disorders are also treatable using the compounds of the present invention.
• Such non cancerous proliferative disorders include, for example, stenosis, restenosis, in-stent stenosis, vascular graft restenosis, arthritis, rheumatoid arthritis, diabetic retinopathy, angiogenesis, pulmonary fibrosis, hepatic cirrhosis, atherosclerosis, glomerulonephritis, diabetic nephropathy, thrombic microangiopathy syndromes and transplant rejection.
• the inflammatory disease or disorder that can be treated with the compounds of the present invention includes, for example, polymyositis, septic/toxic shock, acute respiratory distress syndrome (ARDS), asthma, systemic lupus erythematosus, dermatitis (contact hypersensitivity), peritoneal inflammation, delayed-type hypersensitivity reactions, reperfusion injury, bum injury, transplant rejection, chronic inflammatory disease, hemorragic-traumatic shock, metastases, etc.
• conjugates described herein are useful for the treatment of chronic inflammatory diseases and disorders including, but not limited to, inflammatory bowel diseases such as Crohn's disease and ulcerative colitis, Behcet's disease, polymyositis, Reiter's syndrome, psoriatic arthritis, systemic lupus erythematosus and vasculitis.
• chronic inflammatory diseases and disorders including, but not limited to, inflammatory bowel diseases such as Crohn's disease and ulcerative colitis, Behcet's disease, polymyositis, Reiter's syndrome, psoriatic arthritis, systemic lupus erythematosus and vasculitis.
• the skin disease or disorder that can be treated with the compounds of the present invention includes, but is not limited to, psoriasis, atopical dermatitis, contact dermatitis and further eczematous dermatitises, seborrhoeic dermatitis, Lichen planus, Pemphigus, bullous Pemphigoid, Epidermolysis bullosa, urticaria, angioedemas, vasculitides, erythemas, cutaneous eosinophilias, Lupus erythematosus and acne.
• the compounds described herein are in particular useful for the treatment of psoriasis.
• immune disease or disorder refers to any disease or disorder associated with a development of an immune reaction, either a cellular or a humoral immune reaction, or both, and/or which affects the immune system.
• immune diseases and disorders include inflammatory diseases and disorders, allergy and autoimmune diseases.
• Non-limiting examples of immune diseases include demyelinating diseases that are characterized by a demyelinating process of the central nervous system, such as, e.g., multiple sclerosis, sub-acute sclerosing panencephalomyelitis (SSPE), metachromatic leukodystrophy, inflammatory demyelinating polyradiculoneuropathy, Pelizaeus-Merzbacher disease and Guillain-Barre syndrome.
• demyelinating diseases that are characterized by a demyelinating process of the central nervous system, such as, e.g., multiple sclerosis, sub-acute sclerosing panencephalomyelitis (SSPE), metachromatic leukodystrophy, inflammatory demyelinating polyradiculoneuropathy, Pelizaeus-Merzbacher disease and Guillain-Barre syndrome.
• SSPE sub-acute sclerosing panencephalomyelitis
• metachromatic leukodystrophy e.g
• autoimmune disease generally relates to an immune disease wherein the immune response is developed against antigens normally present in the affected patient, for example an organ specific autoimmune disease (an immune response specifically directed against for example, the endocrine system, the hematopoietic system, the skin, the cardiopulmonary system, the neuromuscular system, the central nervous system, etc) or a systemic autoimmune disease, e.g., Systemic lupus erythematosous, Rheumatoid arthritis, polymyositis, transplant rejection etc.
• organ specific autoimmune disease an immune response specifically directed against for example, the endocrine system, the hematopoietic system, the skin, the cardiopulmonary system, the neuromuscular system, the central nervous system, etc
• a systemic autoimmune disease e.g., Systemic lupus erythematosous, Rheumatoid arthritis, polymyositis, transplant rejection etc.
• the compounds described herein are in particular useful for the treatment of autoimmune diseases or
• the pharmaceutical composition provided by the present invention may be prepared by conventional techniques, e.g., as described in Remington: The Science and Practice of Pharmacy, 19th Ed., 1995.
• the composition may be in solid, semisolid or liquid form and may further include pharmaceutically acceptable fillers, carriers or diluents, and other inert ingredients and excipients.
• the pharmaceutical composition can be designed for a slow release of the conjugate.
• the composition can be administered by any suitable route, e.g. intravenously, orally, parenterally, rectally, or transdermally. The dosage will depend on the state of the patient, and will be determined as deemed appropriate by the practitioner.
• the route of administration may be any route which effectively transports the active compound to the appropriate or desired site of action, the oral route being preferred.
• a solid carrier is used for oral administration, the preparation may be tabletted, placed in a hard gelatin capsule in powder or pellet form or it can be in the form of a lozenge.
• a liquid carrier is used, the preparation may be in the form of a syrup, emulsion or soft gelatin capsule. Tablets, dragees or capsules having talc and/or a carbohydrate carrier or binder or the like are particularly suitable for oral application.
• Preferable carriers for tablets, dragees or capsules include lactose, com starch and/or potato starch.
• the present invention relates to use of a compound as defined above, or a pharmaceutically acceptable salt thereof, for the treatment of a disease or disorder selected from a proliferative disease or disorder, an inflammatory disease or disorder, a skin disease or disorder, or an immune disease or disorder.
• the present invention provides a method for treatment of a disease or disorder selected from a proliferative disease or disorder, an inflammatory disease or disorder, a skin disease or disorder, or an immune disease or disorder, said method comprising administering to an individual in need a therapeutically effective amount of a compound as defined above or a pharmaceutically acceptable salt thereof.
• Preferred compounds for use in the method of the present invention are SA- 6MP and SA-6MPR, or pharmaceutically acceptable salts thereof.
• Alliin was synthesized as previously described (Stoll and Seebeck, 1951). Allicin was produced by applying synthetic alliin on an immobilized alliinase column (Miron et al, 2006), and the concentration was determined by HPLC as previously described (Miron et al., 2002). 2-nitro 5-thio-benzoic acid (NTB) was prepared as previously described (Miron et al, 1998).
• Mass spectra were recorded on a Micromass Platform LCZ 4000 Mass Spectrometer Instrument, using ESI-Electro Spray Ionization Mode, at the following conditions: samples were directly infused at 5 ⁇ l/minute, maintaining the nitrogen flow at 360 liters/hour; the capillary used was 4.16 KV; the cone voltage was 43 V, and the extractor voltage was 4 V; the source block temperature was kept at 100 0 C and the desolvation temperature was kept at 150 0 C; LM RES 14.4; HM RES 14.4; and Ion Energy 0.5. NMR experiments were performed on a Bruker Avance-500 spectrometer. SA-6MP and SA-6MPR were dissolved in CDCl 3 at about 5-10 mM.
• N87 a human gastric adenocarcinoma cell line
• HeIa HtTA-I cells a human cervix carcinoma cell line
• clone HtTA-I Gossen and Bujard, 1992
• MDR HT-29 a human colon adenocarcinoma cell line.
• DMEM Dulbecco modified Eagle's medium
• FCS heat-inactivated fetal calf serum
• All the other cells were grown in suspension: among them were established cell lines such as HL60, a human leukocyte promyelocytic leukemia; U937, human myelomonocytic cells; MOLT4, a T-lymphoblastic cell line derived from acute lymphoblastic leukemia; Jurkat, a human T cell, lymphoblast-like cell; Daudi, a B-lymphoblastoid cell line derived from Burkitt lymphoma.
• HL60 a human leukocyte promyelocytic leukemia
• U937 human myelomonocytic cells
• MOLT4 a T-lymphoblastic cell line derived from acute lymphoblastic leukemia
• Jurkat a human T cell, lymphoblast-like cell
• Daudi a B-lymphoblastoid cell line derived from Burkit
• peripheral blood mononuclear cells were obtained from heparinized whole blood drawn from patients at Rai stage IV with their written consent. Blood cells were subjected to ficoll density gradient centrifugation and the mononuclear cells were diluted to the desired concentration. The cells in suspensions were maintained in RPMI- 1640 supplemented with 2 mM L-glutamine, antibiotics and 10% (v/v) heat-inactivated fetal calf serum (FCS).
• FCS heat-inactivated fetal calf serum
• Cell proliferation was determined by the XTT viability assay in 96-well plates, based on the reduction of tetrazolium salt to soluble formazan compounds by living cells.
• Cells 10,000-15,000 cells/well
• 50 ⁇ l of XTT/PMS mixture 50 ⁇ M PMS, 0.1% XTT in medium
• SDS 1%, 10 ⁇ l/well was added to reference wells before adding the XTT/PMS solution.
• Adhesive cells (N87, HeIa HtTA-I and MDR HT-29) were seeded at 10,000 cells/well (96-well plate) or 60,000 cells/well (24-well plate). Cells in suspension (B-CLL, Daudi, HL-60, Jurkat, MOLT4 and U937cells) were seeded at 15,000 cells/well (96-wells plate) or 100,000 cells/well (24-wells plate). Adhesive cells were grown at 37 0 C for 6 h after seeding, before treatment.
• B-CLL cells were incubated with FITC-CD 19 anti-human antibodies (Becton Dickinson, NJ, USA) for 20 minutes at 4 0 C. After washing off the unbound antibodies, samples were incubated with 5 ⁇ l Annexin-Cy5 (Pharmingen, San Diego, CA, USA) in 10 mM HEPES pH 7.4 buffer containing 140 mM NaCl and 2.5 mM CaCl 2 , (HBS) for 10 minutes at room temperature.
• Annexin-Cy5 Pharmingen, San Diego, CA, USA
• PI propidium iodide
• SA-6MP S-allylthio-6-mercaptopurine
• SA-6MPR S- aHylthio-6-mercaptopurine 9-riboside
• SA-6MP S-allylthio-6-merca ⁇ topurine
• SA-6MPR S-allylthio-6-mercaptopurine 9-riboside
• SA-6MP molecular weight 2234
• SA-6MPR appeared as white crystals (ESI-MS: molecular weight 356), its maximum absorbance in ethanol at 284 run E M 284 was 14,240 M -1 Cm "1 .
• 6-MP and SA-6MP were applied to Daudi, HeIa and N87 cells cultured in 96-well plates in the presence of [ 3 H] thymidine, as described in Materials and Methods.
• SA-6MP inhibited DNA synthesis at a much higher efficacy than 6-MP and in all the cell lines tested, treatment resulted in a dose-dependent inhibition of cell proliferation.
• the sensitivity to the prodrug is cell type dependent.
• 50% inhibition was observed at about 20 ⁇ M, compared to 100 ⁇ M in N87 cells and 110 ⁇ M in HeIa cells. 6-MP caused no inhibition of proliferation, at the same concentrations.
• the trypan blue dye exclusion test was used to assess cell death.
• different concentrations of 6MP and SA-6MP were applied to various cell cultures (Daudi and HeIa cells, each at 30,000 cells/well) as described in Materials and Methods.
• the cells were stained with trypan blue to monitor the lethal effect of several concentrations of the tested conjugates as the percentage of dead cells.
• cell death induced by 6-MP and SA-6MP was both concentration and cell type dependent.
• MDR HT-29 cells were treated for 16 h with 150 ⁇ M of either 6-
• B-CLL human peripheral blood mononuclear cells PBMC
• 6-MP or SA-6MP peripheral blood mononuclear cells
• Sorting showed that there was no significant increase in the percent of apoptotic cells treated for 16 h with 6-MP at the range between 0-150 ⁇ M (Figs. 5A-5D); however, when following treatment with 50 and 100 ⁇ M SA-6MP, the percent of apoptotic cells increased to 38 and 95%, respectively (Figs. 5E-5F).
• apoptosis FACS analysis with annexin
• cell death trypan blue test
• Daudi cells and N87 cells grown in the presence of 6-MP or 6-MPR (0-100 ⁇ M) showed no significant loss of cell proliferation.
• a slightly decreased proliferation was observed for N87 cells at 150 ⁇ M (6-MP ⁇ 80%; 6-MPR ⁇ 75%, p ⁇ 0.05).
• SA-6MP had a very potent antiproliferative effect on Daudi cells, reducing their proliferation to 15-30% at 50 ⁇ M.
• the residual proliferation was 50-60%. Treatment with SA-6MPR showed similar results.
• the residual proliferation of Daudi cells-treated with SA-6MPR at 50 or 100 ⁇ M was 60% and 25%, respectively, whereas in N87 cells treated with SA-6MPR at 50 or 100 ⁇ M, the residual viability was about 70% and 55%, respectively. There was a complete loss of proliferation in N87 cells treated with SA-6MPR at 150 ⁇ M.
• the concentration effects of 6-MP, 6-MPR SA-6MP and SA-6MPR on cell proliferation were used to assess the efficacy of the different drugs in various cell lines.
• cell in suspension namely, Daudi, HL-60, U937, Molt-4, Jurkat and B-CLL
• monolayer cells namely, HeIa HtTA-I, MDR HT-29 and N87
• cell viability was determined by trypan blue dye exclusion assay. Data are presented in Table 2 hereinbelow for 6MP derivatives-treated for 16 h.
• B-CLL cells from human peripheral blood mononuclear cells were highly resistant to the treatment. Nevertheless, the FACS results, indicating apoptotic processes in progress (Figs. 5E-5F), suggest that longer incubation times may result in cell death.
• the monolayer cell lines tested were less sensitive to SA-6MP and SA-6MPR than cells in suspension.
• the calculated IC 5O of 6-MP for most of the cell lines tested was higher than 200 ⁇ M under a 16 h exposure to the drug. This might seem a rather high concentration, as compared with other results reported in the nM range.
• the IC 50 values after 4 days of treatment with azathioprine (AZ) or 6-MP were 230.4 ⁇ 231.3 and 149.5 ⁇ 124.9 nM, respectively.
• Example 3 The efficacy of mercaptopurine derivatives in treatment of colitis
• the anti-inflammatory activity of the compounds of the present invention in examined in vivo, using mice in which chronic inflammation of the colon has been induced as a model for colitis.
• the chronic infalammation is induced either by intrarectal administration of 2,4,6- trinitrobenzene sulfonic acid (TNBS) (80 mg/kg body weight, dissolved in 0.9% NaCl 5 30 ⁇ l/mouse) or by administration of dextran sodium sulphate (DSS, 2% w/v) in the drinking water for 5 days.
• TNBS 2,4,6- trinitrobenzene sulfonic acid
• DSS dextran sodium sulphate
• mice are treated with various amounts of either 6-MP or a compound of the present invention, or with a control vehicle, and the development of inflammation is followed 7-13 days after the induction of colitis. Development of Colitis is assessed daily by measurement of body weight and stool consistency. At the end of the experimentmt, mice are sacrificed by cervical dislocation. The colon length and the histological parameters are used to evaluate the efficacy of the various treatments. Macroscopic lesion analysis is performed as previously described (Wallace et al, 1989).

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