WO2008102743A1 - 新規なα－ガラクトシダーゼ - Google Patents

新規なα－ガラクトシダーゼ Download PDF

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Publication number
WO2008102743A1
WO2008102743A1 PCT/JP2008/052691 JP2008052691W WO2008102743A1 WO 2008102743 A1 WO2008102743 A1 WO 2008102743A1 JP 2008052691 W JP2008052691 W JP 2008052691W WO 2008102743 A1 WO2008102743 A1 WO 2008102743A1
Authority
WO
WIPO (PCT)
Prior art keywords
galactosidase
novel
raffinose
attaining
galactose
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/052691
Other languages
English (en)
French (fr)
Inventor
Shinichi Imazu
Kazunobu Konishi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Asahi Kasei Chemicals Corp
Original Assignee
Asahi Kasei Chemicals Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asahi Kasei Chemicals Corp filed Critical Asahi Kasei Chemicals Corp
Priority to CN200880005393.8A priority Critical patent/CN101657544B/zh
Priority to JP2009500177A priority patent/JP5006380B2/ja
Publication of WO2008102743A1 publication Critical patent/WO2008102743A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

　本発明の目的は、選択的にラフィノースを合成することが可能な新規α－ガラクトシダーゼを提供することである。本発明によれば、スクロースとガラクトースを原料とした脱水縮合反応において、生成オリゴ糖中のラフィノース含有率が０．５％以上のとき、ラフィノース合成選択率が６５％以上という性質を有するα－ガラクトシダーゼが提供される。
PCT/JP2008/052691 2007-02-19 2008-02-19 新規なα－ガラクトシダーゼ Ceased WO2008102743A1 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN200880005393.8A CN101657544B (zh) 2007-02-19 2008-02-19 新型α-半乳糖苷酶
JP2009500177A JP5006380B2 (ja) 2007-02-19 2008-02-19 新規なα−ガラクトシダーゼ

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2007037505 2007-02-19
JP2007-037504 2007-02-19
JP2007-037505 2007-02-19
JP2007037504 2007-02-19

Publications (1)

Publication Number Publication Date
WO2008102743A1 true WO2008102743A1 (ja) 2008-08-28

Family

ID=39710020

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/052691 Ceased WO2008102743A1 (ja) 2007-02-19 2008-02-19 新規なα－ガラクトシダーゼ

Country Status (4)

Country Link
JP (1) JP5006380B2 (ja)
CN (1) CN101657544B (ja)
TW (1) TW200846472A (ja)
WO (1) WO2008102743A1 (ja)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009148189A (ja) * 2007-12-20 2009-07-09 Asahi Kasei Chemicals Corp 新規なα−ガラクトオリゴ糖の製造方法
WO2017202997A1 (en) * 2016-05-24 2017-11-30 Novozymes A/S Compositions comprising polypeptides having galactanase activity and polypeptides having beta-galactosidase activity
CN110066780A (zh) * 2019-03-29 2019-07-30 上海国龙生物技术集团有限公司 一种饲用α-半乳糖苷酶固体发酵生产工艺
US10947520B2 (en) 2016-05-24 2021-03-16 Novozymes A/S Compositions comprising polypeptides having galactanase activity and polypeptides having beta-galactosidase activity
CN114181925A (zh) * 2020-09-14 2022-03-15 武汉禾元生物科技股份有限公司 一种重组蛋白酶k工业化纯化及冻干方法

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5236967B2 (ja) * 2008-03-06 2013-07-17 旭化成ケミカルズ株式会社 Ｂａｃｉｌｌｕｓｃｏａｇｕｌａｎｓに属する菌株を用いたメリビオースの製造方法
CN101974583A (zh) * 2010-09-20 2011-02-16 南京农业大学 一种利用α-半乳糖苷酶合成α-低聚半乳糖的方法
US10808268B2 (en) * 2016-05-24 2020-10-20 Novozymes A/S Polypeptides having alpha-galactosidase activity and polynucleotides encoding same
CN106282142B (zh) * 2016-08-05 2019-05-31 南京林业大学 一种β-甘露糖苷酶含量低的α-半乳糖苷酶的制备方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001321179A (ja) * 2000-05-15 2001-11-20 Nippon Beet Sugar Mfg Co Ltd ラフィノースの新規製造方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001321179A (ja) * 2000-05-15 2001-11-20 Nippon Beet Sugar Mfg Co Ltd ラフィノースの新規製造方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
AJISAKA K. ET AL.: "Synthetic studies of oligosaccharides by use of a reversed hydrolysis activity of glycohydrolases", DENPUN KAGAKU, vol. 36, no. 2, 1989, pages 113 - 122 *
DEY P.M.: "Transgalactosylation activity of sweet almond alpha-galactosidases: Synthesis of saccharides", PHYTOCHEMISTRY, vol. 18, 1979, pages 35 - 38 *
GANTER C. ET AL.: "Production of thermostable, recombinant alpha-galactosidase suitable for raffinose elimination from sugar beet syrup", JOURNAL OF BIOTECHNOLOGY, vol. 8, 1988, pages 301 - 310 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009148189A (ja) * 2007-12-20 2009-07-09 Asahi Kasei Chemicals Corp 新規なα−ガラクトオリゴ糖の製造方法
WO2017202997A1 (en) * 2016-05-24 2017-11-30 Novozymes A/S Compositions comprising polypeptides having galactanase activity and polypeptides having beta-galactosidase activity
CN109196098A (zh) * 2016-05-24 2019-01-11 诺维信公司 包含具有半乳聚糖酶活性的多肽和具有β-半乳糖苷酶活性的多肽的组合物
US10927359B2 (en) 2016-05-24 2021-02-23 Novozymes A/S Compositions comprising polypeptides having galactanase activity and polypeptides having beta-galactosidase activity
US10947520B2 (en) 2016-05-24 2021-03-16 Novozymes A/S Compositions comprising polypeptides having galactanase activity and polypeptides having beta-galactosidase activity
CN110066780A (zh) * 2019-03-29 2019-07-30 上海国龙生物技术集团有限公司 一种饲用α-半乳糖苷酶固体发酵生产工艺
CN114181925A (zh) * 2020-09-14 2022-03-15 武汉禾元生物科技股份有限公司 一种重组蛋白酶k工业化纯化及冻干方法

Also Published As

Publication number Publication date
TWI363093B (ja) 2012-05-01
CN101657544B (zh) 2014-01-29
TW200846472A (en) 2008-12-01
JP5006380B2 (ja) 2012-08-22
JPWO2008102743A1 (ja) 2010-05-27
CN101657544A (zh) 2010-02-24

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