WO2008093762A1 - 低分子量ペプチドの高感度定量方法 - Google Patents

低分子量ペプチドの高感度定量方法 Download PDF

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Publication number
WO2008093762A1
WO2008093762A1 PCT/JP2008/051472 JP2008051472W WO2008093762A1 WO 2008093762 A1 WO2008093762 A1 WO 2008093762A1 JP 2008051472 W JP2008051472 W JP 2008051472W WO 2008093762 A1 WO2008093762 A1 WO 2008093762A1
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WO
WIPO (PCT)
Prior art keywords
protein removal
tca
molecular weight
low molecular
determination method
Prior art date
Application number
PCT/JP2008/051472
Other languages
English (en)
French (fr)
Inventor
Naoaki Murao
Masaki Ishigai
Original Assignee
Chugai Seiyaku Kabushiki Kaisha
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Chugai Seiyaku Kabushiki Kaisha filed Critical Chugai Seiyaku Kabushiki Kaisha
Priority to JP2008556163A priority Critical patent/JP5276993B2/ja
Publication of WO2008093762A1 publication Critical patent/WO2008093762A1/ja

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)

Abstract

　前処理方法としてTCA除タンパク法に着目しLC-MS/MSを行った。血漿中のPTH類似体、CNP-22の測定について、TCA除タンパク処理した後LC-MS/MSを行った場合と、他の除タンパク法の場合と結果を比較した。TCA除タンパク法は、他の前処理方法と比較して非常に高い回収率を示した。また、プロダクトイオンに単一アミノ酸由来のインモニウムイオンを用いるSRM測定を組み合わせることで高感度測定が可能となった。本方法のバリデーション結果からは、本発明の方法が十分な再現性および真度のみならず、高感度の方法であることが示された。本発明の方法は、病気の診断や薬物動態試験にも応用可能である。
PCT/JP2008/051472 2007-01-31 2008-01-31 低分子量ペプチドの高感度定量方法 WO2008093762A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2008556163A JP5276993B2 (ja) 2007-01-31 2008-01-31 低分子量ペプチドの高感度定量方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-022347 2007-01-31
JP2007022347 2007-01-31

Publications (1)

Publication Number Publication Date
WO2008093762A1 true WO2008093762A1 (ja) 2008-08-07

Family

ID=39674069

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/051472 WO2008093762A1 (ja) 2007-01-31 2008-01-31 低分子量ペプチドの高感度定量方法

Country Status (2)

Country Link
JP (1) JP5276993B2 (ja)
WO (1) WO2008093762A1 (ja)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010204050A (ja) * 2009-03-05 2010-09-16 Shimadzu Corp マトリックス支援レーザ脱離イオン化質量分析用マトリックス
JP2015118029A (ja) * 2013-12-19 2015-06-25 国立大学法人東北大学 生体試料中のビオチンまたはその関連物質の測定方法
JP2016075698A (ja) * 2008-08-08 2016-05-12 クエスト ダイアグノスティックス インヴェストメンツ インコーポレイテッド 血漿レニンの質量分析法
JP2017134077A (ja) * 2011-12-05 2017-08-03 クエスト ダイアグノスティックス インヴェストメンツ インコーポレイテッド 質量分析による逆トリヨードサイロニンの検出法
CN108663245A (zh) * 2018-05-17 2018-10-16 北京和合医学诊断技术股份有限公司 精确检测血液中谷氨酸和天冬氨酸含量的血液处理方法
CN111108377A (zh) * 2017-07-14 2020-05-05 株式会社Ｍｃｂｉ 疾病检测方法
CN113702463A (zh) * 2021-08-31 2021-11-26 重庆医科大学国际体外诊断研究院 一种快速检测血浆硫酸吲哚酚的电化学方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002505740A (ja) * 1997-05-22 2002-02-19 オックスフォード・グリコサイエンシーズ・（ユーケイ）・リミテッド 新たなペプチド配列決定法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002505740A (ja) * 1997-05-22 2002-02-19 オックスフォード・グリコサイエンシーズ・（ユーケイ）・リミテッド 新たなペプチド配列決定法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ALDO RODA ET AL.: "Development and validation of a sensitive HPLC-ESI-MS/MS method for the direct determination of glucosamine in human plasma", JOURNAL OF CHROMATOGRAPHY B, vol. 844, no. 1, 21 November 2003 (2003-11-21), pages 119 - 126, XP005755353 *
CHEN Z.-J. ET AL.: "Selective method for the determination of cefdinir in human plasma using liquid chromatography electrospray ionization tandem mass spectrometry", JOURNAL OF CHROMATOGRAPHY, vol. 834, no. 1-2, 13 April 2006 (2006-04-13), pages 163 - 169, XP005367464 *
CHERLET M. ET AL.: "Control of the keto-enol tautomerism of chlortetracycline for its straight forward quantitation in pig tissues by liquid chromatography-elecrospray ionization tandem mass spectrometry", JOURNAL OF CHROMATOGRAPHY, vol. 1133, no. 1-2, 10 November 2006 (2006-11-10), pages 135 - 141, XP005713005 *
POLSON C. ET AL.: "Optimization of protein precipitation based upon effectiveness of protein removal and ionization effect in liquid chromatography-tandem mass spectrometry", JOURNAL OF CHROMATOGRAPHY B, vol. 785, no. 2, 5 March 2003 (2003-03-05), pages 263 - 275, XP004404026 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016075698A (ja) * 2008-08-08 2016-05-12 クエスト ダイアグノスティックス インヴェストメンツ インコーポレイテッド 血漿レニンの質量分析法
JP2010204050A (ja) * 2009-03-05 2010-09-16 Shimadzu Corp マトリックス支援レーザ脱離イオン化質量分析用マトリックス
JP2017134077A (ja) * 2011-12-05 2017-08-03 クエスト ダイアグノスティックス インヴェストメンツ インコーポレイテッド 質量分析による逆トリヨードサイロニンの検出法
JP2015118029A (ja) * 2013-12-19 2015-06-25 国立大学法人東北大学 生体試料中のビオチンまたはその関連物質の測定方法
CN111108377A (zh) * 2017-07-14 2020-05-05 株式会社Ｍｃｂｉ 疾病检测方法
CN108663245A (zh) * 2018-05-17 2018-10-16 北京和合医学诊断技术股份有限公司 精确检测血液中谷氨酸和天冬氨酸含量的血液处理方法
CN113702463A (zh) * 2021-08-31 2021-11-26 重庆医科大学国际体外诊断研究院 一种快速检测血浆硫酸吲哚酚的电化学方法

Also Published As

Publication number Publication date
JPWO2008093762A1 (ja) 2010-05-20
JP5276993B2 (ja) 2013-08-28

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