WO2008053019A3 - Procédé de réduction de l'expression d'un gène dans une cellule fongique filamenteuse - Google Patents

Procédé de réduction de l'expression d'un gène dans une cellule fongique filamenteuse Download PDF

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Publication number
WO2008053019A3
WO2008053019A3 PCT/EP2007/061768 EP2007061768W WO2008053019A3 WO 2008053019 A3 WO2008053019 A3 WO 2008053019A3 EP 2007061768 W EP2007061768 W EP 2007061768W WO 2008053019 A3 WO2008053019 A3 WO 2008053019A3
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Prior art keywords
filamentous fungal
gene
expression
fungal cell
cell
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PCT/EP2007/061768
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English (en)
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WO2008053019A2 (fr
Inventor
Van Noel Nicolaas Maria Eisabeth Peij
Lucie Parenicova
Mojca Bencina
Karolina Ivicak
Original Assignee
Dsm Ip Assets Bv
Peij Van Noel Nicolaas Maria Elisabeth
Lucie Parenicova
Mojca Bencina
Karolina Ivicak
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Application filed by Dsm Ip Assets Bv, Peij Van Noel Nicolaas Maria Elisabeth, Lucie Parenicova, Mojca Bencina, Karolina Ivicak filed Critical Dsm Ip Assets Bv
Publication of WO2008053019A2 publication Critical patent/WO2008053019A2/fr
Publication of WO2008053019A3 publication Critical patent/WO2008053019A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/12—Applications; Uses in screening processes in functional genomics, i.e. for the determination of gene function

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un procédé de réduction de l'expression d'un gène dans une cellule fongique filamenteuse. L'invention concerne en outre une cellule fongique filamenteuse modifiée appropriée pour la production d'un composé d'intérêt, dans laquelle, par comparaison avec la cellule mère de la cellule modifiée issue de celle-ci, ladite cellule modifiée présente des capacités améliorées pour la réduction de l'expression génique d'un gène cible à médiation par l'ARNi. De plus, l'invention concerne un procédé pour la fabrication d'un procédé d'intérêt dans ladite cellule fongique filamenteuse modifiée.
PCT/EP2007/061768 2006-11-02 2007-10-31 Procédé de réduction de l'expression d'un gène dans une cellule fongique filamenteuse WO2008053019A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06123393.8 2006-11-02
EP06123393 2006-11-02

Publications (2)

Publication Number Publication Date
WO2008053019A2 WO2008053019A2 (fr) 2008-05-08
WO2008053019A3 true WO2008053019A3 (fr) 2009-01-22

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PCT/EP2007/061768 WO2008053019A2 (fr) 2006-11-02 2007-10-31 Procédé de réduction de l'expression d'un gène dans une cellule fongique filamenteuse

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WO (1) WO2008053019A2 (fr)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2010275672A1 (en) 2009-07-22 2012-02-02 Dsm Ip Assets B.V. Improved host cell for the production of a compound of interest
EP2588616B1 (fr) 2010-07-01 2018-11-14 DSM IP Assets B.V. Procédé de production d'un composé particulier
MX357482B (es) 2012-07-19 2018-07-11 Dsm Ip Assets Bv Cepa deficiente en agse.
WO2014028833A1 (fr) * 2012-08-17 2014-02-20 Novozymes A/S Méthodes pour le co-silençage de l'expression de gènes dans des souches fongiques filamenteuses et leurs utilisations
AU2014273055A1 (en) 2013-05-31 2015-12-17 Dsm Ip Assets B.V. Microorganisms for diterpene production
WO2014202624A2 (fr) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Gène de rasamsonia et son utilisation
WO2014202622A2 (fr) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Gène de rasamsonia et son utilisation
WO2014202620A2 (fr) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Gène de rasamsonia et son utilisation
US10590436B2 (en) 2015-01-06 2020-03-17 Dsm Ip Assets B.V. CRISPR-CAS system for a lipolytic yeast host cell
WO2016110453A1 (fr) 2015-01-06 2016-07-14 Dsm Ip Assets B.V. Système crispr-cas pour cellule hôte fongique filamenteuse
EP3242949B1 (fr) 2015-01-06 2021-11-03 DSM IP Assets B.V. Système crispr-cas destiné à une cellule hôte de lévure
CN109715804A (zh) 2016-09-23 2019-05-03 帝斯曼知识产权资产管理有限公司 用于宿主细胞的指导rna表达系统
WO2018166943A1 (fr) 2017-03-13 2018-09-20 Dsm Ip Assets B.V. Souche déficientes en régulateur transcriptionnel d'amas binucléaire de zinc
CN110462044A (zh) 2017-04-06 2019-11-15 帝斯曼知识产权资产管理有限公司 自引导整合构建体(sgic)
US20200392513A1 (en) 2017-12-20 2020-12-17 Dsm Ip Assets B.V. A method for genome editing in a host cell
WO2019063849A1 (fr) 2017-12-20 2019-04-04 Dsm Ip Assets B.V. Purification d'un polypeptide d'intérêt
CN112088215A (zh) 2018-05-09 2020-12-15 帝斯曼知识产权资产管理有限公司 Crispr瞬时表达构建体(ctec)
US20220056460A1 (en) 2018-12-05 2022-02-24 Dsm Ip Assets B.V. Crispr guide-rna expression strategies for multiplex genome engineering
EP3966324A1 (fr) 2019-05-06 2022-03-16 DSM IP Assets B.V. Donneur de crispr à plusieurs parties
US20220332803A1 (en) * 2019-08-09 2022-10-20 Siemens Healthcare Diagnostics Inc. Anti-hog tcn1 monoclonal antibodies and methods of production and use thereof
US20220389458A1 (en) 2019-11-04 2022-12-08 Dsm Ip Assets B.V. Low volume transfection
AU2021367159A1 (en) 2020-10-22 2023-05-04 Cargill, Incorporated Microorganisms for diterpene production

Citations (3)

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Publication number Priority date Publication date Assignee Title
WO2005056772A1 (fr) * 2003-12-09 2005-06-23 Novozymes Inc. Procedes permettant d'eliminer ou de reduire l'expression de genes dans des souches fongiques filamenteuses
EP1681347A1 (fr) * 2005-01-18 2006-07-19 Metanomics GmbH & Co. KGaA Procédés ameliorés pour le silençage génique assuré par l'ARN double-brins
WO2007056826A1 (fr) * 2005-11-21 2007-05-24 Johnson & Johnson Research Pty Limited Arns interferents de multiciblage et methodes d'utilisation et de conception

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005056772A1 (fr) * 2003-12-09 2005-06-23 Novozymes Inc. Procedes permettant d'eliminer ou de reduire l'expression de genes dans des souches fongiques filamenteuses
EP1681347A1 (fr) * 2005-01-18 2006-07-19 Metanomics GmbH & Co. KGaA Procédés ameliorés pour le silençage génique assuré par l'ARN double-brins
WO2007056826A1 (fr) * 2005-11-21 2007-05-24 Johnson & Johnson Research Pty Limited Arns interferents de multiciblage et methodes d'utilisation et de conception

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