WO2008026295A1 - Supporter - Google Patents

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Publication number
WO2008026295A1
WO2008026295A1 PCT/JP2006/317807 JP2006317807W WO2008026295A1 WO 2008026295 A1 WO2008026295 A1 WO 2008026295A1 JP 2006317807 W JP2006317807 W JP 2006317807W WO 2008026295 A1 WO2008026295 A1 WO 2008026295A1
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WO
WIPO (PCT)
Prior art keywords
extract
support
mass
cellulose
water
Prior art date
Application number
PCT/JP2006/317807
Other languages
French (fr)
Japanese (ja)
Inventor
Kinya Takagaki
Original Assignee
Toyo Shinyaku Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyo Shinyaku Co., Ltd. filed Critical Toyo Shinyaku Co., Ltd.
Priority to PCT/JP2006/317807 priority Critical patent/WO2008026295A1/en
Priority to JP2008531948A priority patent/JPWO2008026295A1/en
Publication of WO2008026295A1 publication Critical patent/WO2008026295A1/en

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/141—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers
    • A61K9/146—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers with organic macromolecular compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
    • A61K47/38—Cellulose; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/0208—Tissues; Wipes; Patches
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73—Polysaccharides
    • A61K8/731—Cellulose; Quaternized cellulose derivatives

Definitions

  • the present invention relates to a support having excellent water retention, comprising cellulose having a network structure.
  • cellulose produced by microorganisms include agar-like substances known as “Nata de Coco” in foods. This substance has a network structure in which fine cell mouth fibers having a diameter of 1 ⁇ or less are entangled randomly.
  • cellulose having a network structure is used as a raw material for cosmetics, pharmaceuticals, and foodstuffs V ⁇ , which is also expected as a next-generation liquid crystal raw material (JP-A-62-1). 7 5 1 90 and JP-A-10-310 60 1).
  • Cellulose having such a network structure has water retention. For example, it is difficult to adsorb or absorb functional components between fibers (inside the mesh), and these components are simply on the surface. It only adheres. Disclosure of the invention
  • the present inventor has intensively studied in view of the above problems.
  • the support capable of holding a specific amount or more of water relative to the dry mass of the support is a desired component in the solution or dispersion.
  • the present invention is a support made of cellulose having a network structure, the support Provided is a support capable of holding 20 times or more of water with respect to the dry mass of the body.
  • the present invention also provides an M-enriched composition obtained by immersing the support in a solution or dispersion containing a desired component.
  • the wet composition is in sheet form.
  • the component is a cosmetic ingredient.
  • the support of the present invention can adsorb or absorb the desired component in the solution or dispersion by immersing it in a solution or dispersion containing the desired component.
  • a wet composition containing a functional component can be obtained.
  • the support of the present invention is made of cellulose having a network structure.
  • 90% by mass or more of the dry mass of the support is preferably cellulose and its derivatives.
  • Such a support can be obtained, for example, by culturing a microorganism that produces cellulose.
  • microorganisms that produce cellulose include microorganisms belonging to the genus Acetopacter, Syudomonas, and Agrobacterium. Among them, the genus Acetopacter is preferable because of the large amount of cellulose produced.
  • Examples of the culture method include stationary culture and stirring culture.
  • stationary culture performed without stirring the culture solution, cellulose produced by microorganisms accumulates on the surface of the culture solution to form a cellulose membrane having a network structure. Made. As the culture progresses, a film-like support made of thickened cellulose is obtained.
  • the stationary culture is preferable in that the cellulose having a network structure can be easily recovered and the cellulose is in a sheet form.
  • the culture conditions for obtaining the support of the present invention can be normal culture conditions.
  • the culture solution contains carbon sources, nitrogen sources, inorganic salts, and amino acids and vitamins as necessary, as nutrients for microorganisms.
  • grains containing these nutrients rice, wheat, etc.
  • vegetables cabbage, ghoul, onion, etc.
  • fruits cabbage, ghoul, onion, etc.
  • the pH in the culture can be appropriately set according to the microorganism to be used, and is preferably about pH 2 to 9, more preferably about pH 2 to 7.
  • the thus-prepared culture solution is inoculated with the above-mentioned cellulose-producing microorganism, and preferably static culture is performed.
  • the culture temperature is preferably 10 ° C to 40 ° C. C, more preferably 25 ° C. to 40 ° C.
  • the culture period is preferably 1 day to 60 days, more preferably 5 days to 14 days.
  • the obtained cellulose having a network structure can be used as it is as the support of the present invention.
  • cellulose having a network structure obtained from a microorganism belonging to the genus Acetopacter has a strong acetic acid odor, it is preferably washed repeatedly with water until the washing solution becomes near neutral.
  • the support of the present invention has a water retention amount of 20 times or more with respect to the dry mass of the support.
  • the water retention amount can be obtained, for example, by the following method. First, obtained After immersing the support in water for 7 days, the mass is measured. This mass is the saturated wet mass. Next, the support saturated with water is freeze-dried, and the mass after drying is measured. The water retention amount can be obtained by subtracting the mass after drying from the saturated wet mass.
  • the support of the present invention is preferably obtained in a sheet form. By being obtained in a sheet form in this way, it is easy to process into various forms. Further, a sheet-like support having a thickness of about 20 mm may be sliced to form a thin sheet-like support.
  • the thickness of the support is not particularly limited.
  • the mass of the support is 95% by mass with respect to the saturated wet mass from the viewpoint that the component is efficiently adsorbed or absorbed in the sheet by dipping in a solution or dispersion. In the above state, the thickness is preferably 5 mm or less.
  • the wet composition of the present invention can be obtained by immersing the above support in a solution or dispersion containing a desired component.
  • a solution or dispersion containing a desired component For example, when a support saturated with water is immersed in a solution or dispersion containing the desired component, the solution or dispersion immersed in water in the support (desired component concentration 0 mass%) The concentration of the desired component is in an equilibrium state, and a wet composition in which the desired component in the solution or dispersion is adsorbed or absorbed inside the support can be obtained.
  • a certain amount of water from the support it may be immersed in a solution or dispersion containing a desired component to obtain a wet composition.
  • the efficiency with which the desired component is adsorbed or absorbed on the support can be increased.
  • 1 mass of the saturated wet mass of the support. / 0 or more, preferably 3% by mass or more of water may be removed.
  • the mass of the support is preferably 15% by mass or more, more preferably 30% by mass of the saturated wet mass.
  • moisture can be removed so as to be more preferably 50% by mass or more, and most preferably 80% by mass or more. If moisture is removed until the mass of the support is less than 10% by mass of the saturated wet mass, the water absorption efficiency of the support may be deteriorated.
  • the method for removing (dehydrating) water is not particularly limited, and examples thereof include heat drying, decompression concentration, pressing, and centrifugation. Among these, it is preferable to remove water by pressing.
  • the time for immersing the support in the solution or the dispersion to obtain the wet composition of the present invention can be appropriately set depending on the thickness of the support, the solution used, the dispersion, and the like. Preferably it is 1 hour or more, more preferably 3 hours or more. It may also be soaked for a long time (for example, for a month or more). Just do it.
  • the solvent of the solution or dispersion used for preparing the wet composition of the present invention is not particularly limited as long as it does not break the cellulose network structure.
  • a solvent Preferably, polar solvents, such as water and ethanol, are mentioned.
  • Desired ingredients include pharmaceutical raw materials, food raw materials, or cosmetic raw materials, which are dissolved or dispersed in the above solvents.
  • food ingredients include royal jelly, propolis, vitamins (A,
  • beverages containing the above-mentioned food ingredients for example, plant fermented juice, vegetable juice (for example, carrot juice), fruit juice, and the like can also be used.
  • seasonings such as sugar liquid and sugar alcohol can be added to enhance sweetness.
  • Cosmetic raw materials include cosmetic base materials and cosmetic auxiliaries.
  • Such cosmetic raw materials include alcohols such as ethanol; polyhydric alcohols such as 1,3-butylene glycol and glycerin; fats and oils such as hardened castor oil and olive oil; polyethylene glycol, silicone, and cross-linked silicone Examples include base materials related to the feeling of use such as polyacrylic acid and solubilized cellulose (polymer compounds, etc.); preservatives such as phenoxetanol and paraben; surfactants such as acylglycine.
  • components having a skin improvement function include whitening agents, antioxidants, blood circulation promoters, moisturizers, anti-inflammatory agents, cell activators, antibacterial agents, enzyme inhibitors, and UV inhibitors.
  • the whitening agent one having tyrosinase activity or one having an activity of decolorizing melanin pigment is mainly used.
  • the whitening agent include L-cystein and its derivatives, ascorbic acid and its derivatives, tranexamic acid derivatives, grablizine, liquiritin, isoliquiritin, hydroquinone, alptin and kojic acid.
  • an extract having a whitening effect can also be used as a whitening agent. Examples of such an extract include Sohakuhi extract, Toki extract, Ibukitorano extract, Caquette extract, Clara extract, Hawthorn extract, For example, white lily extract, hop extract, and rose extract.
  • Antioxidants include, for example, carotenoids such as vitamin A, coenzyme Q 10, hypolipoic acid, vitamin B, asconolevic acid, vitamin E, L monocystine, and derivatives thereof, and salts thereof.
  • carotenoids such as vitamin A, coenzyme Q 10, hypolipoic acid, vitamin B, asconolevic acid, vitamin E, L monocystine, and derivatives thereof, and salts thereof.
  • Examples include riboflavin, SOD, mannitol, hydroquinone, cholesterol, tryptophan, histidine, catechin and its derivatives, gallic acid and its derivatives, BHT, ⁇ ⁇ ⁇ , proanthocyanin, gallotannin and the like.
  • a plant extract having an antioxidant action as an antioxidant can also be used. Examples of such an extract include pine bark extract, grape seed extract, yew leaf extract, potampi extract, parsley extract. , Plant extracts such as melissa extract and argon extract.
  • the blood circulation promoter examples include carpronium chloride, dialkyl monoamine derivatives, vitamin ⁇ and its derivatives, and asparasaponin.
  • an extract having a blood circulation promoting action can also be used as a blood circulation promoting agent.
  • examples of such an extract include an Al two force extract, a sempri extract, a life-prolonging grass. Extract, kujin extract, eel extract, citrus extract, koka extract, salamander extract, ginger extract, citrus extract, tanjin extract, chixen ginseng extract, ginseng extract, toki extract Products, beech extract, bowie extract and the like.
  • humectants include polyhydric alcohols such as ethylene glycol, glycerin, erythritol, phenoxyethanol; glucose, galactose, trehalose, sorbitol (sorbitol), chitin derivatives, chitosan derivatives, mucopolysaccharides Sugars such as lecithin, phosphatidylglycerol, phosphatidylserine, sphingomyelin; Amino acids such as lidonic carboxylic acids; oils and fats such as olive oil, jojoba oil, and squalane; cyclodextrin derivatives, collagen that is fibrous protein, elastin, Click, placenta, sterols is intercellular lipid, betaine, such as natural products moisturizing factors such as urea.
  • polyhydric alcohols such as ethylene glycol, glycerin, erythritol, phenoxyethanol
  • glucose galactose
  • mucopolysaccharides examples include hyaluronic acid, chondroitin sulfate, dermatan sulfate, heparan sulfate, heparin, keratan sulfate, and salts thereof.
  • Anti-inflammatory agents include, for example, glycyrrhizic acid, glycyrrhetinic acid, mephuminamic acid, phenylbutazone, indomethacin, ibuprofenic acid, ketoporophene, allantoin, guaiazulene, and derivatives thereof, ⁇ -aminocaproic acid, zinc oxide, diclofenac sodium, Examples include proanthocyanidins.
  • an extract having an anti-inflammatory action can be used as an anti-inflammatory agent.
  • Examples of such an extract include a yukinoshita extract, a licorice extract, an aloe extract, a sicon extract, a salvia extract, an alni Force extract, forcelet extract, birch extract, hypericum extract, eucalyptus extract, Examples include extracts of animals and plants such as mulberry extract, peach leaf extract, pine bark extract, bud seed extract, and bilberry extract.
  • Cell activators include, for example, deoxyribonucleic acid and its salts, adenosine triphosphates, adenylate derivatives such as adenosine monophosphate and their salts, ribonucleic acid and its salts, guanine, xanthine, their Examples thereof include nucleic acid substances such as derivatives.
  • an extract having a cell activating effect can also be used as a cell activator, and examples of such extracts include serum protein extract, placenta extract, spleen extract, royal jelly, carrot extract, and sempli extract. , Rosemary extract, opac extract, garlic extract, hinokitiol, cephalanthin and other animal and plant extracts; and yeast, lactic acid bacteria, ganoderma and other microorganism-derived extracts.
  • Antibacterial agents for example, erythromycin, clindamycin, gentamicin, penicillin, Kuroramufue Nicole, antibiotics such as tetracycline; benzo I helper O key side; nadifloxacin; ethanol; chloride benzalkonium Roh record Yu arm; Iou; c 0
  • Examples include lahydroxybenzoate estenoles; salicinolic acid; hinokitiol monole; triclosan; homosnorefamine; chloramine T; catechins and their derivatives; chitin / chitosan derivatives.
  • an extract having an antibacterial action can also be used as an antibacterial agent, and examples of such an extract include wikiweed, opac, chia, rose hip, force mitsu, orange, sagittarius, turmeric, gardenia, and chioji. Extracts such as sage, cypress, clove, eucalyptus, cypress, cedar.
  • Enzyme inhibitors are mainly inhibitors of degrading enzymes that degrade collagen, elastin, hyaluronic acid, etc., which make up the skin's skin.
  • the enzyme inhibitor include vitamin C, vitamin E, catechin and derivatives thereof.
  • extracts having an enzyme inhibitory action are also used as enzyme inhibitors.
  • Such extracts include: turmeric, sorghum, zea oy, thyme, yacon, rosehip, rosemary, cypress, peony, guarana, guava, acacia, grape, bilberry, yew, Examples include extracts such as lingo, tomato and pine bark.
  • UV inhibitors examples include paraaminobenzoic acid, salicylic acid homoester,
  • the wet composition in which the food material is adsorbed or absorbed may be used as it is.
  • the wet composition may be dried and powdered to be processed into tablets, granules, or various forms. You may use as a raw material of a processed product.
  • the wet composition in which the cosmetic raw material is adsorbed or absorbed may be used as it is, and may be processed into forms such as quasi-drugs, cosmetics and toiletries.
  • Quasi-drugs, cosmetics, toiletries, etc. for example, cosmetic creams, liquids, creams, packs, patches, hair tonics, hair creams, shampoos, hair rinses, treatments, body shampoos, facial cleansers, sarcophagus, foundations
  • Example 1 First, 25 g of glucose, 5 g of yeast extract, and 3 g of peptone were dissolved in 100 OmL of purified water. Next, the pH of the obtained aqueous solution was adjusted to 6, and sterilized in an autoclave to prepare a medium. Subsequently, 'Acetobacter xylinum IF013693) was seeded on this medium l O OmL, and static culture was performed at 25 ° C for 14 days. After 14 days, the cellulose film formed on the surface of the culture was collected. The collected membrane was thoroughly washed with water and boiled with 1 200 mL of 2% sodium hydroxide solution for 1 hour. Next, the membrane was washed with water until neutral, and a cellulose membrane 1 having a network structure was obtained.
  • the cellulose membrane 1 was saturated with water and pressed into a 1 g square sheet.
  • the water-saturated cellulose membrane 1 obtained by cutting was freeze-dried and the dry mass was measured to find 0.04 g. That is, it was found that the amount of water that can be retained in the cellulose membrane 1 is at least 20 times the dry mass.
  • the cellulose membrane 1 saturated with water was used as the support 1. .
  • a medium was prepared by adding commercially available black vinegar to 1% by mass of glucose. Subsequently, the microorganism used in Example 1 was inoculated into this medium lO 2 OmL, and statically cultured at 25 ° C for 1 month to obtain a cellulose membrane. This cellulose membrane had less odor than the cellulose membrane 1 obtained in Example 1. This cell mouth membrane was washed in the same procedure as in Example 1 to obtain a cellulose membrane 2 having a network structure.
  • Cellulose membrane 1 obtained in Example 1 was saturated with water and pressed into a 1 g square sheet. Next, water-saturated cellulose obtained by cutting Membrane 1 was squeezed while measuring the amount of water removed. When 0.3 g of water was removed, the pressing was stopped, and the cellulose membrane 1 obtained by cutting was prepared so that the mass of the cellulose membrane 1 was 70% by mass of the saturated wet mass. Next, the cellulose membrane 1 obtained by this cutting (that is, cellulose membrane 1 having a mass of 70% by mass of saturated wet mass) was immersed in purified water for a while and its mass was measured. 9 8 g. As described in Example 1, the dry weight of the cellulose membrane 1 is 0.04 g.
  • the amount of water that can be retained in the cellulose membrane 1 having a mass of 70% by mass of the saturated wet mass is 20 times or more the dry mass.
  • Cellulose membrane 1 having a mass of 70% by mass of the saturated wet mass was used as support 2.
  • Cellulose membrane 1 obtained in Example 1 was saturated with water and pressed into a 1 g square sheet. Next, the water-saturated cellulose membrane 1 obtained by cutting was compressed while measuring the amount of water removed. When 0.9 g of water was removed, the pressing was stopped, and the cellulose membrane 1 obtained by cutting was prepared so that the mass of the cellulose membrane 1 was 10% by mass of the saturated wet mass. Next, the cellulose membrane 1 obtained by this cutting (that is, a cellulose membrane 1 having a mass of 10% by mass of saturated wet mass) was immersed in purified water for a while and its mass was measured. It was 3 g. As described in Example 1, the dry weight of the cellulose membrane 1 is 0.04 g.
  • the support 2 and the support 3 obtained in Example 3 and Comparative Example 1 were mixed with coloring pigments (trade name: Anchor Red SP 500, manufactured by Yaegaki Fermentation Co., Ltd.), respectively. It was immersed in 20 mL of an aqueous solution containing 1% by mass. Next, the support 2 and the support 3 are taken out from the aqueous solution, and the surfaces of the respective supports are washed with purified water to obtain a wet composition 1 (derived from the support 2) and a wet composition 2 (derived from the support 3). It was. As a result of visual comparison of the color strength of the wet composition 1 and the wet composition 2, the wet composition 1 was darker than the wet yarn 1 and the composition 2. Therefore, it was found that the support 2 adsorbs or absorbs the coloring pigment (component) more efficiently than the support 3.
  • coloring pigments trade name: Anchor Red SP 500, manufactured by Yaegaki Fermentation Co., Ltd.
  • Example 1 The support 1 obtained in Example 1 was immersed in an aqueous solution containing the following components to obtain a wet composition 3 (unit: mass Z volume%).
  • the functional component in the solution or dispersion can be adsorbed or absorbed efficiently.
  • a wet composition containing a functional component can be obtained.
  • Such a support and a wetting composition have excellent functionality and moisture retention, and are useful as base materials for pharmaceuticals, foods, quasi drugs, cosmetics, toiletries, and the like.

Abstract

A supporter of cellulose with network structure, exhibiting a water retention capacity of 20 times or more the dry mass of supporter. This supporter, through immersion in a dispersion or solution containing a functional component, is capable of efficiently adsorbing or absorbing the functional component contained in the dispersion or solution. For example, there can be obtained a wet composition containing a functional component. The obtained supporter or wet composition has excellent functioning and moisture retaining capabilities, thereby being useful as a base material of any of pharmaceuticals, food, quasi drugs, cosmetics, toiletry products, etc.

Description

明 細 書 支持体 技術分野  Technical support Technical field
本発明は、 網目構造を有するセルロースからなる、 保水性に優れた支持体 に関する。 背景技術  The present invention relates to a support having excellent water retention, comprising cellulose having a network structure. Background art
微生物が産生するセルロースとしては、 例えば食品では、 「ナタデココ」 として知られる寒天様の物質が挙げられる。 この物質は、 直径が 1 πι以下 の微細なセル口ース繊維がランダムに絡み合あつた網目構造を有している。 近年、 網目構造を有するセルロースを、 化粧品、 医薬品、 食品の材料ある Vヽは原料として用いることが提案されており、 次世代の液晶原料としても期 待されている （特開昭 6 2— 1 7 5 1 9 0号公報および特開平 1 0— 3 1 0 6 0 1号公報を参照） 。  Examples of cellulose produced by microorganisms include agar-like substances known as “Nata de Coco” in foods. This substance has a network structure in which fine cell mouth fibers having a diameter of 1πι or less are entangled randomly. In recent years, it has been proposed that cellulose having a network structure is used as a raw material for cosmetics, pharmaceuticals, and foodstuffs V ヽ, which is also expected as a next-generation liquid crystal raw material (JP-A-62-1). 7 5 1 90 and JP-A-10-310 60 1).
このような網目構造を有するセルロースは、 保水性を有している。 し力、し、 非常に微細な繊維が絡み合つているため、 例えば、 機能性を有する成分を繊 維間 （網目内） に吸着または吸収させることは難しく、 このような成分は単 に表面に付着するにすぎない。 発明の開示  Cellulose having such a network structure has water retention. For example, it is difficult to adsorb or absorb functional components between fibers (inside the mesh), and these components are simply on the surface. It only adheres. Disclosure of the invention
本発明者は、 上記課題を鑑み、 鋭意検討を行ったところ、 支持体の乾燥質 量に対して特定の量以上の水を保持し得る支持体は、 溶液中または分散液中 の所望の成分を支持体内部に吸着または吸収し得ることを見出した。  The present inventor has intensively studied in view of the above problems. As a result, the support capable of holding a specific amount or more of water relative to the dry mass of the support is a desired component in the solution or dispersion. Has been found to be adsorbed or absorbed inside the support.
本発明は、 網目構造を有するセルロースからなる支持体であって、 該支持 体の乾燥質量に対して、 2 0倍以上の質量の水を保持し得る支持体を提供す る。 The present invention is a support made of cellulose having a network structure, the support Provided is a support capable of holding 20 times or more of water with respect to the dry mass of the body.
本発明はまた、 所望の成分を含有する溶液または分散液に上記支持体を浸 漬して得られる、 M潤組成物を提供する。  The present invention also provides an M-enriched composition obtained by immersing the support in a solution or dispersion containing a desired component.
1つの実施態様においては、 上記湿潤組成物は、 シート状である。  In one embodiment, the wet composition is in sheet form.
1つの実施態様においては、 上記成分は、 化粧品原料である。  In one embodiment, the component is a cosmetic ingredient.
本発明の支持体は、 所望の成分を含有する溶液または分散液に浸漬するこ とにより、 該溶液中または分散液中の所望の成分を該支持体内部に吸着また は吸収することができるため、 例えば、 機能性成分を含有する湿潤組成物を 得ることができる。  The support of the present invention can adsorb or absorb the desired component in the solution or dispersion by immersing it in a solution or dispersion containing the desired component. For example, a wet composition containing a functional component can be obtained.
発明を実施するための最良の形態 BEST MODE FOR CARRYING OUT THE INVENTION
以下、 本発明の支持体および湿潤組成物について説明する。  Hereinafter, the support and the wet composition of the present invention will be described.
(支持体） (Support)
本発明の支持体は、 網目構造を有するセルロースからなる。 支持体中の網 目構造を有する成分は、 支持体の乾燥質量中の 9 0質量％以上がセルロース およびその誘導体であることが好ましい。  The support of the present invention is made of cellulose having a network structure. As for the component having a network structure in the support, 90% by mass or more of the dry mass of the support is preferably cellulose and its derivatives.
このような支持体は、 例えば、 セルロースを産生する微生物を培養するこ とによって得られる。 このようなセルロースを産生する微生物としては、 ァ セトパクター属、 シユードモナス属、 ァグロパクテリゥム属などに属する微 生物が挙げられる。 この中でもセルロースの産生量が多い点から、 ァセトパ クター属が好ましい。  Such a support can be obtained, for example, by culturing a microorganism that produces cellulose. Examples of such microorganisms that produce cellulose include microorganisms belonging to the genus Acetopacter, Syudomonas, and Agrobacterium. Among them, the genus Acetopacter is preferable because of the large amount of cellulose produced.
培養方法としては、 静置培養、 撹拌培養などが挙げられる。 例えば、 培養 液を撹拌せずに行う静置培養の場合、 微生物によって産生されたセルロース が培養液の表面に集積することにより、 網目構造を有するセルロース膜が形 成される。 培養が進むにつれて肥厚したセルロースからなる膜状の支持体が 得られる。 網目構造を有するセルロースを容易に回収でき、 このセルロース がシート状である点で、 静置培養が好ましい。 Examples of the culture method include stationary culture and stirring culture. For example, in the case of stationary culture performed without stirring the culture solution, cellulose produced by microorganisms accumulates on the surface of the culture solution to form a cellulose membrane having a network structure. Made. As the culture progresses, a film-like support made of thickened cellulose is obtained. The stationary culture is preferable in that the cellulose having a network structure can be easily recovered and the cellulose is in a sheet form.
本発明の支持体を得るための培養条件は、 通常の培養条件であり得る。 培 養液は、 微生物の栄養素として炭素源、 窒素源、 無機塩類、 必要に応じてァ ミノ酸、 ビタミンなどを含有する。 特に、 これらの栄養分を含有している穀 物 （コメ、 ムギなど） 、 野菜 （キャベツ、 グール、 タマネギなど） 、 果物 The culture conditions for obtaining the support of the present invention can be normal culture conditions. The culture solution contains carbon sources, nitrogen sources, inorganic salts, and amino acids and vitamins as necessary, as nutrients for microorganisms. In particular, grains containing these nutrients (rice, wheat, etc.), vegetables (cabbage, ghoul, onion, etc.), fruits
(ミカンなどの柑橘類、 イチゴ、 パイナップルなど） などを培地成分として 用いると、 得られるセルロースの臭いが少なく、 安全性の点からも好ましい。 穀物の発酵により得られる酢を培地成分として用いてもよい。 培養における p Hは、 用いる微生物に応じて適宜設定され得、 好ましくは p H 2〜9、 よ り好ましくは p H 2〜 7程度である。 Use of citrus fruits such as mandarin oranges, strawberry, pineapple, etc. as a medium component is preferable from the viewpoint of safety because the resulting cellulose has a small odor. Vinegar obtained by cereal fermentation may be used as a medium component. The pH in the culture can be appropriately set according to the microorganism to be used, and is preferably about pH 2 to 9, more preferably about pH 2 to 7.
このように調製された培養液に、 上述のセルロースを産生する微生物を播 種し、 好ましくは静置培養を行う。 培養温度は、 好ましくは 1 0 °C〜 4 0。C、 より好ましくは 2 5 °C~ 4 0 °Cである。 培養の期間は、 好ましくは 1日〜 6 0日、 より好ましくは 5日〜 1 4日である。 このようにして培養することで、 微生物が産生したセルロースがランダムに絡み合い、 網目構造を有するセル ロースを得ることができる。  The thus-prepared culture solution is inoculated with the above-mentioned cellulose-producing microorganism, and preferably static culture is performed. The culture temperature is preferably 10 ° C to 40 ° C. C, more preferably 25 ° C. to 40 ° C. The culture period is preferably 1 day to 60 days, more preferably 5 days to 14 days. By culturing in this way, cellulose produced by microorganisms can be randomly entangled and cellulose having a network structure can be obtained.
得られた網目構造を有するセルロースは、 そのまま本発明の支持体として 利用し得る。 しかし、 セルロースが絡み合う過程で、 培養液中の成分、 微生 物由来の有機酸などを含有しているため、 水で洗浄することが好ましい。 例 えば、 ァセトパクター属に属する微生物から得られた網目構造を有するセル ロースは、 強い酢酸臭を有するため、 洗浄液が中性付近となるまで水で繰り 返して洗浄することが好ましい。  The obtained cellulose having a network structure can be used as it is as the support of the present invention. However, it is preferable to wash with water because it contains components in the culture solution, organic acids derived from microorganisms, and the like in the process of intertwining cellulose. For example, since cellulose having a network structure obtained from a microorganism belonging to the genus Acetopacter has a strong acetic acid odor, it is preferably washed repeatedly with water until the washing solution becomes near neutral.
本発明の支持体は、 支持体の乾燥質量に対して 2 0倍以上の保水量を有す る。 保水量は、 例えば、 以下の方法で求めることができる。 まず、 得られた 支持体を、 7日間水に浸漬した後、 質量を測定する。 この質量を飽和湿質量 とする。 次いで、 水で飽和させた支持体を、 凍結乾燥させて、 乾燥後の質量 を測定する。 飽和湿質量から乾燥後の質量を差し引くことにより保水量が求 められる。 The support of the present invention has a water retention amount of 20 times or more with respect to the dry mass of the support. The water retention amount can be obtained, for example, by the following method. First, obtained After immersing the support in water for 7 days, the mass is measured. This mass is the saturated wet mass. Next, the support saturated with water is freeze-dried, and the mass after drying is measured. The water retention amount can be obtained by subtracting the mass after drying from the saturated wet mass.
本発明の支持体はシート状の形態で得られることが好ましい。 このように シート状で得られることにより、 様々な形態に加工し易い。 さらに、 2 0 m m程度の厚みを有するシート状の支持体を、 スライスすることによって、 厚 みの薄いシート状の支持体としてもよい。 支持体の厚みは、 特に制限されず、 例えば、 溶液または分散液に浸漬して成分をシートに効率よく吸着または吸 収させる点から、 支持体の質量が飽和湿質量に対して 9 5質量％以上の状態 で、 厚みは 5 mm以下であることが好ましい。  The support of the present invention is preferably obtained in a sheet form. By being obtained in a sheet form in this way, it is easy to process into various forms. Further, a sheet-like support having a thickness of about 20 mm may be sliced to form a thin sheet-like support. The thickness of the support is not particularly limited. For example, the mass of the support is 95% by mass with respect to the saturated wet mass from the viewpoint that the component is efficiently adsorbed or absorbed in the sheet by dipping in a solution or dispersion. In the above state, the thickness is preferably 5 mm or less.
(湿潤組成物） (Wet composition)
本発明の湿潤組成物は、 上記支持体を、 所望の成分を含有する溶液または 分散液に浸漬することにより得られる。 例えば、 水で飽和させた支持体を、 所望の成分を含有する溶液または分散液に浸漬すると、 支持体中の水 （所望 の成分濃度 0質量％) と浸漬する溶液または分散液 （成分を任意の濃度で含 む） との間で所望の成分の濃度が平衡状態となり、 溶液中または分散液中の 所望の成分が支持体内部に吸着または吸収された湿潤組成物を得ることがで きる。 あるいは、 支持体から一定量の水分を除去 （脱水） した後、 所望の成 分を含有する溶液または分散液に浸漬して湿潤組成物を得てもよい。 このよ うに支持体から一定量の水分を除去すると、 所望の成分が支持体に吸着また は吸収される効率を上げることができる。 支持体から一定量の水分を除去す る場合、 水分を完全に除去する必要はなく、 支持体の飽和湿質量の 1質量。 /0 以上、 好ましくは 3質量％以上の水分を除去すればよい。 例えば、 支持体の 質量が飽和湿質量の好ましくは 1 5質量％以上、 より好ましくは 3 0質量％ 以上、 さらに好ましくは 5 0質量％以上、 最も好ましくは 8 0質量％以上と なるように水分を除去し得る。 支持体の質量が飽和湿質量の 1 0質量％未満 となるまで水分を除去すると、 支持体の吸水効率が悪くなるおそれがある。 支持体の質量を飽和湿質量の 1 0質量％未満とする場合には、 支持体中に含 まれる水を、 水以外の溶媒 （以下に説明） に置換することが好ましい。 水分 を除去 （脱水） する方法についても特に制限されず、 例えば、 加熱乾燥、 減 圧濃縮、 圧搾、 遠心分離などが挙げられる。 これらの中でも、 圧搾によって 水分を除去することが好ましい。 The wet composition of the present invention can be obtained by immersing the above support in a solution or dispersion containing a desired component. For example, when a support saturated with water is immersed in a solution or dispersion containing the desired component, the solution or dispersion immersed in water in the support (desired component concentration 0 mass%) The concentration of the desired component is in an equilibrium state, and a wet composition in which the desired component in the solution or dispersion is adsorbed or absorbed inside the support can be obtained. Alternatively, after removing (dehydrating) a certain amount of water from the support, it may be immersed in a solution or dispersion containing a desired component to obtain a wet composition. Thus, by removing a certain amount of moisture from the support, the efficiency with which the desired component is adsorbed or absorbed on the support can be increased. When removing a certain amount of water from the support, it is not necessary to completely remove the water, 1 mass of the saturated wet mass of the support. / 0 or more, preferably 3% by mass or more of water may be removed. For example, the mass of the support is preferably 15% by mass or more, more preferably 30% by mass of the saturated wet mass. As described above, moisture can be removed so as to be more preferably 50% by mass or more, and most preferably 80% by mass or more. If moisture is removed until the mass of the support is less than 10% by mass of the saturated wet mass, the water absorption efficiency of the support may be deteriorated. When the mass of the support is less than 10% by mass of the saturated wet mass, it is preferable to replace the water contained in the support with a solvent other than water (described below). The method for removing (dehydrating) water is not particularly limited, and examples thereof include heat drying, decompression concentration, pressing, and centrifugation. Among these, it is preferable to remove water by pressing.
本発明の湿潤組成物を得るために支持体を溶液中または分散液中に浸漬す る時間は、 支持体の厚さ、 用いる溶液、 分散液などによって適宜設定され得 る。 好ましくは 1時間以上、 より好ま'しくは 3時間以上である。 また、 長期 間 （例えば 1ヶ月以上） 浸漬してもよく、 この場合は、 溶液または分散液、 あるいは支持体に腐食が生じない期間で行う力、 あるいは溶液または分散液 に防腐剤を添加して行えばよい。  The time for immersing the support in the solution or the dispersion to obtain the wet composition of the present invention can be appropriately set depending on the thickness of the support, the solution used, the dispersion, and the like. Preferably it is 1 hour or more, more preferably 3 hours or more. It may also be soaked for a long time (for example, for a month or more). Just do it.
本発明の湿潤組成物の調製に用いる溶液または分散液の溶媒は、 セルロー スの網目構造を破壌しない溶媒であれば特に制限されない。 溶媒としては、 好ましくは、 水、 エタノールなどの極性溶媒が挙げられる。  The solvent of the solution or dispersion used for preparing the wet composition of the present invention is not particularly limited as long as it does not break the cellulose network structure. As a solvent, Preferably, polar solvents, such as water and ethanol, are mentioned.
所望の成分としては、 医薬品原料、 食品原料、 または化粧品原料が挙げら れ、 上記の溶媒に溶解または分散されている。  Desired ingredients include pharmaceutical raw materials, food raw materials, or cosmetic raw materials, which are dissolved or dispersed in the above solvents.
例えば食品原料としては、 ローヤルゼリー、 プロポリス、 ビタミン類 （A、 For example, food ingredients include royal jelly, propolis, vitamins (A,
C、 D、 E、 K、 葉酸、 パントテン酸、 ビォチン、 これらの誘導体など） 、 ミネラル （鉄、 マグネシウム、 カルシウム、 亜鉛など） 、 アミノ酸 （バリン、 ロイシン、 イソロイシン、 グリシン、 プロリン、 ァノレギニン、 グノレタミン、 L—カル-チン、 γ—ァミノ酪酸など） 、 セレン、 キチン 'キトサン、 レシ チン、 ポリフヱノール （カテキン類、 プロアントシァニジン、 ェラグ酸類、 ガロタンニン、 フラポノイド類および誘導体、 クロロゲン酸類など） 、 カロ テノイド （リコピン、 ァスタキサンチン、 ゼアキサンチン、 ルティンなど） 、 キサンチン誘導体 （カフェインなど） 、 脂肪酸、 タンパク質 （コラーゲン、 エラスチンなど） 、 ムコ多糖類 （ヒアルロン酸、 コンドロイチン、 デルマタ ン、 へパラン、 へパリン、 ケタラン、 これらの塩など） 、 ァミノ糖 （ダルコ サミン、 ァセチルダルコサミン、 ガラクトサミン、 ァセチルガラタトサミン、 ノィラミン酸、 ァセチルノイラミン酸、 へキソサミン、 それらの塩など） 、 オリゴ糖 （イソマルトオリゴ糖、 環状オリゴ糖など） 、 リン脂質、 スフイン ゴ脂質およびその誘導体 （ホスファチジルコリン、 スフインゴミエリン、 セ ラミドなど） 、 ュビキノン （コェンザィム Q 1 0など） 、 α—リポ酸、 含硫 化合物 （ァリイン、 セパェン、 タウリン、 グルタチオン、 メチルスルホニノレ メタンなど） 、 糖アルコール （還元麦芽糖、 還元パラチノース、 エリスリ ト ールなど） 、 リグナン類 （セサミンなど） 、 これらを含有する動植物抽出物、 根菜類 （ゥコン、 ショウガなど） 、 麦若葉末などのイネ科植物の緑葉、 ケー ルなどのアブラナ科植物の緑葉などの抽出物が挙げられる。 さらに、 上記食 品原料を含む飲料、 例えば、 植物発酵ジュース、 野菜ジュース （例えば、 人 参ジュース） 、 果汁なども利用され得る。 また、 糖液、 糖アルコールなどの 調味料を加えて甘味を強くすることもできる。 C, D, E, K, folic acid, pantothenic acid, biotin, derivatives of these), minerals (iron, magnesium, calcium, zinc, etc.), amino acids (valine, leucine, isoleucine, glycine, proline, anoleginine, gnoretamine, L —Car-tin, γ-aminobutyric acid, etc.), selenium, chitin 'chitosan, lecithin, polyphenol (catechins, proanthocyanidins, ellagic acids, gallotannins, flavonoids and derivatives, chlorogenic acids, etc.), caro Tenoids (lycopene, astaxanthin, zeaxanthin, rutin, etc.), xanthine derivatives (such as caffeine), fatty acids, proteins (collagen, elastin, etc.), mucopolysaccharides (hyaluronic acid, chondroitin, dermatan, heparan, heparin, Ketalan, their salts, etc.), amino sugars (darcosamine, acetyl darcosamine, galactosamine, acetyl galatatosamine, neurolamic acid, acetilyl neuraminic acid, hexosamine, their salts, etc.), oligosaccharide (isomalto-oligo) sugars, such as cyclic oligosaccharides), phospholipids, Sufuin Gore lipids and derivatives thereof (phosphatidylcholine, staple Ingo myelin, etc. ceramide), etc. Yubikinon (Koenzaimu Q 1 0), alpha - lipoic acid, sulfur-containing compound (Ariin Sepane, Taurine, Glutathione, Methylsulfoninomethane, etc., Sugar alcohol (Reduced maltose, Reduced palatinose, Erythritol, etc.), Lignans (Sesamin, etc.), Animal and plant extracts containing these, Root vegetables (Tucon, Ginger) And green leaves of gramineous plants such as wheat powder, and green leaves of cruciferous plants such as kale. Furthermore, beverages containing the above-mentioned food ingredients, for example, plant fermented juice, vegetable juice (for example, carrot juice), fruit juice, and the like can also be used. In addition, seasonings such as sugar liquid and sugar alcohol can be added to enhance sweetness.
化粧品原料としては、 化粧品基材、 化粧品助剤などが挙げられる。 このよ うな化粧品原料としては、 エタノールなどのアルコール類； 1， 3—ブチレ ングリコール、 グリセリンなどの多価アルコール類；硬化ヒマシ油、 オリー ブ油などの油脂類；ポリエチレングリコール、 シリコン、 架橋型シリコン、 ポリアクリル酸、 可溶化セルロースなどの使用感に関連する基材 （高分子化 合物など） ；フエノキシェタノール、 パラベンなどの防腐剤；ァシルグリシ ンなどの界面活性剤などが挙げられる。 さらに、 皮膚の改善機能を有する成 分としては、 美白剤、 抗酸化剤、 血行促進剤、 保湿剤、 抗炎症剤、 細胞賦活 剤、 抗菌剤、 酵素阻害剤、 紫外線防止剤などが挙げられる。 以下に各有効成 分となり得る物質およびそれらの作用を有する抽出物を例示するが、 これら に限定されるものではない。 さらに、 以下に例示する有効成分の中には、 単 独で様々な作用を有する成分もあるが、 その用途は限定されるものではない。 美白剤は、 チロシナーゼ活性を有するものまたはメラニン色素を脱色する 活性を有するものが主に用いられる。 美白剤としては、 例えば、 L—システ インおよびその誘導体、 ァスコルビン酸おょぴその誘導体、 トラネキサム酸 誘導体、 グラブリジン、 リクイリチン、 イソリクイリチン、 ハイドロキノン、 アルプチン、 コウジ酸などが挙げられる。 さらに、 美白剤として美白作用を 有する抽出物なども用いられ得、 このような抽出物としては、 ソゥハクヒ抽 出物、 トウキ抽出物、 イブキトラノォ抽出物、 ケィケットウ抽出物、 クララ 抽出物、 サンザシ抽出物、 シラユリ抽出物、 ホップ抽出物、 ノイバラ抽出物 などが挙げられる。 Cosmetic raw materials include cosmetic base materials and cosmetic auxiliaries. Such cosmetic raw materials include alcohols such as ethanol; polyhydric alcohols such as 1,3-butylene glycol and glycerin; fats and oils such as hardened castor oil and olive oil; polyethylene glycol, silicone, and cross-linked silicone Examples include base materials related to the feeling of use such as polyacrylic acid and solubilized cellulose (polymer compounds, etc.); preservatives such as phenoxetanol and paraben; surfactants such as acylglycine. In addition, examples of components having a skin improvement function include whitening agents, antioxidants, blood circulation promoters, moisturizers, anti-inflammatory agents, cell activators, antibacterial agents, enzyme inhibitors, and UV inhibitors. Each effective Examples of substances that can be used as components and extracts having these actions are exemplified, but the present invention is not limited thereto. In addition, some of the active ingredients exemplified below have single and various actions, but their uses are not limited. As the whitening agent, one having tyrosinase activity or one having an activity of decolorizing melanin pigment is mainly used. Examples of the whitening agent include L-cystein and its derivatives, ascorbic acid and its derivatives, tranexamic acid derivatives, grablizine, liquiritin, isoliquiritin, hydroquinone, alptin and kojic acid. Furthermore, an extract having a whitening effect can also be used as a whitening agent. Examples of such an extract include Sohakuhi extract, Toki extract, Ibukitorano extract, Caquette extract, Clara extract, Hawthorn extract, For example, white lily extract, hop extract, and rose extract.
抗酸化剤としては、 例えば、 ビタミン Aなどのカロテノイド類、 コェンザ ィム Q 1 0、 ひーリポ酸、 ビタミン B類、 ァスコノレビン酸、 ビタミン E、 L 一システィン、 およびこれらの誘導体、 ならびにこれらの塩、 リボフラビン、 S O D , マンニトール、 ハイ ドロキノン、 コレステロ一ノレ、 トリプトファン、 ヒスチジン、 カテキンおよびその誘導体、 没食子酸およびその誘導体、 B H T、 Β ΗΑ、 プロアントシァュジン、 ガロタンニンなどが挙げられる。 さら に、 抗酸化剤として抗酸化作用を有する植物抽出物なども用いられ得、 この ような抽出物としては、 松樹皮抽出物、 ブドウ種子抽出物、 イチヨウ葉抽出 物、 ポタンピ抽出物、 パセリ抽出物、 メリッサ抽出物、 ォゥゴン抽出物など の植物抽出物などが挙げられる。  Antioxidants include, for example, carotenoids such as vitamin A, coenzyme Q 10, hypolipoic acid, vitamin B, asconolevic acid, vitamin E, L monocystine, and derivatives thereof, and salts thereof. Examples include riboflavin, SOD, mannitol, hydroquinone, cholesterol, tryptophan, histidine, catechin and its derivatives, gallic acid and its derivatives, BHT, Β プ ロ, proanthocyanin, gallotannin and the like. Furthermore, a plant extract having an antioxidant action as an antioxidant can also be used. Examples of such an extract include pine bark extract, grape seed extract, yew leaf extract, potampi extract, parsley extract. , Plant extracts such as melissa extract and argon extract.
血行促進剤としては、 例えば、 塩化カルプロニゥム、 ジアルキルモノアミ ン誘導体、 ビタミン Εおよびその誘導体、 ァスパラサポニンなどが挙げられ る。 さらに、 血行促進剤として血行促進作用を有する抽出物なども用いられ 得、 このような抽出物としては、 アル二力抽出物、 センプリ抽出物、 延命草 抽出物、 クジン抽出物、 ォウギ抽出物、 カンキヨゥ抽出物、 コゥカ抽出物、 サンショウ抽出物、 ショウキヨウ抽出物、 センキユウ抽出物、 タンジン抽出 物、 チクセッニンジン抽出物、 朝鮮人参抽出物、 トウキ抽出物、 ビヤクシ抽 出物、 ボウイ抽出物などの抽出物などが挙げられる。 Examples of the blood circulation promoter include carpronium chloride, dialkyl monoamine derivatives, vitamin Ε and its derivatives, and asparasaponin. Furthermore, an extract having a blood circulation promoting action can also be used as a blood circulation promoting agent. Examples of such an extract include an Al two force extract, a sempri extract, a life-prolonging grass. Extract, kujin extract, eel extract, citrus extract, koka extract, salamander extract, ginger extract, citrus extract, tanjin extract, chixen ginseng extract, ginseng extract, toki extract Products, beech extract, bowie extract and the like.
保湿剤としては、 例えば、 エチレングリコール、 グリセリン、 エリスリ ト ール、 フエノキシエタノールなどの多価アルコール類；グルコース、 ガラク トース、 トレハロース、 ソルビトール （ソルビット） 、 キチン誘導体、 キト サン誘導体、 ムコ多糖類などの糖類； レシチン、 ホスファチジルグリセロー ル、 ホスファチジルセリン、 スフインゴミエリンなどの脂質；バリン、 ロイ シン、 フエエノレアラニン、 トリプトファン、 セリン、 プロリン、 ヒドロキシ プロリン、 ヒドロキシリジン、 ァノレギニン、 オノレニチン、 ヒスチジン、 ピロ リ ドンカルボン酸などのアミノ酸；ォリーブ油、 ホホパ油、 スクヮランなど の油脂；シクロデキストリン誘導体、 繊維状タンパクであるコラーゲン、 ェ ラスチン、 加水分解シルク、 プラセンタ、 細胞間脂質であるステロール、 ベ タイン、 尿素などの天然物保湿因子などが挙げられる。  Examples of humectants include polyhydric alcohols such as ethylene glycol, glycerin, erythritol, phenoxyethanol; glucose, galactose, trehalose, sorbitol (sorbitol), chitin derivatives, chitosan derivatives, mucopolysaccharides Sugars such as lecithin, phosphatidylglycerol, phosphatidylserine, sphingomyelin; Amino acids such as lidonic carboxylic acids; oils and fats such as olive oil, jojoba oil, and squalane; cyclodextrin derivatives, collagen that is fibrous protein, elastin, Click, placenta, sterols is intercellular lipid, betaine, such as natural products moisturizing factors such as urea.
ムコ多糖類としては、 例えば、 ヒアルロン酸、 コンドロイチン硫酸、 デル マタン硫酸、 へパラン硫酸、 へパリン、 ケラタン硫酸、 これらの塩などが挙 げられる。  Examples of mucopolysaccharides include hyaluronic acid, chondroitin sulfate, dermatan sulfate, heparan sulfate, heparin, keratan sulfate, and salts thereof.
抗炎症剤としては、 例えば、 グリチルリチン酸、 グリチルレチン酸、 メフ ヱナム酸、 フヱニルブタゾン、 インドメタシン、 イブプロフェン酸、 ケトプ 口フェン、 アラントイン、 グアイァズレン、 およびそれらの誘導体、 ε—ァ ミノカプロン酸、 酸化亜鉛、 ジクロフェナクナトリウム、 プロアントシァニ ジンなどが挙げられる。 さらに、 抗炎症剤として抗炎症作用を有する抽出物 なども用いられ得、 このような抽出物としては、 ユキノシタ抽出物、 カンゾ ゥ抽出物、 アロエ抽出物、 シコン抽出物、 サルビア抽出物、 アル二力抽出物、 力ミツレ抽出物、 シラカバ抽出物、 オトギリソゥ抽出物、 ユーカリ抽出物、 ムクロジ抽出物、 モモ葉抽出物、 松樹皮抽出物、 ブド.ゥ種子抽出物、 コケモ モ抽出物などの動植物の抽出物などが挙げられる。 Anti-inflammatory agents include, for example, glycyrrhizic acid, glycyrrhetinic acid, mephuminamic acid, phenylbutazone, indomethacin, ibuprofenic acid, ketoporophene, allantoin, guaiazulene, and derivatives thereof, ε-aminocaproic acid, zinc oxide, diclofenac sodium, Examples include proanthocyanidins. In addition, an extract having an anti-inflammatory action can be used as an anti-inflammatory agent. Examples of such an extract include a yukinoshita extract, a licorice extract, an aloe extract, a sicon extract, a salvia extract, an alni Force extract, forcelet extract, birch extract, hypericum extract, eucalyptus extract, Examples include extracts of animals and plants such as mulberry extract, peach leaf extract, pine bark extract, bud seed extract, and bilberry extract.
細胞賦活剤としては、 例えば、 デォキシリボ核酸おょぴその塩、 アデノシ ン三リン酸、 アデノシン一リン酸などのアデ-ル酸誘導体およびそれらの塩、 リボ核酸およびその塩、 グァニン、 キサンチン、 それらの誘導体などの核酸 物質などが挙げられる。 さらに、 細胞賦活剤として細胞賦活作用を有する抽 出物なども用いられ得、 このような抽出物としては、 血清タンパク抽出物、 胎盤抽出物、 脾臓抽出物、 ローヤルゼリー、 ニンジン抽出物、 センプリ抽出 物、 ローズマリー抽出物、 ォゥパク抽出物、 ニンニク抽出物、 ヒノキチォー ル、 セファランチンなどの動植物抽出物；および酵母、 乳酸菌、 霊芝などの 微生物由来の抽出物が挙げられる。  Cell activators include, for example, deoxyribonucleic acid and its salts, adenosine triphosphates, adenylate derivatives such as adenosine monophosphate and their salts, ribonucleic acid and its salts, guanine, xanthine, their Examples thereof include nucleic acid substances such as derivatives. Furthermore, an extract having a cell activating effect can also be used as a cell activator, and examples of such extracts include serum protein extract, placenta extract, spleen extract, royal jelly, carrot extract, and sempli extract. , Rosemary extract, opac extract, garlic extract, hinokitiol, cephalanthin and other animal and plant extracts; and yeast, lactic acid bacteria, ganoderma and other microorganism-derived extracts.
抗菌剤としては、 例えば、 エリスロマイシン、 クリンダマイシン、 ゲンタ マイシン、 ペニシリン、 クロラムフエ二コール、 テトラサイクリンなどの抗 生物質；ベンゾィルパーォキサイド；ナジフロキサシン；エタノール；塩化 ベンザノレコユウム；ィォゥ；ハ0ラヒドロキシベンゾエートエステノレ類；サリ チノレ酸； ヒノキチォ一ノレ； トリクロサン； ホモスノレファミン； クロラミン T；カテキンおよびそれらの誘導体；キチン ·キトサン誘導体などが挙げら れる。 さらに、 抗菌剤として抗菌作用を有する抽出物なども用いられ得、 こ のような抽出物としては、 ウイキヨウ、 ォゥパク、 チヤ、 ローズヒップ、 力 ミツレ、 オレンジ、 セィヨウノコギリソゥ、 ゥコン、 クチナシ、 チヨウジ、 セージ、 ユキノシタ、 クローブ、 ユーカリ、 ヒノキ、 スギなどの抽出物が挙 げられる。 Antibacterial agents, for example, erythromycin, clindamycin, gentamicin, penicillin, Kuroramufue Nicole, antibiotics such as tetracycline; benzo I helper O key side; nadifloxacin; ethanol; chloride benzalkonium Roh record Yu arm; Iou; c 0 Examples include lahydroxybenzoate estenoles; salicinolic acid; hinokitiol monole; triclosan; homosnorefamine; chloramine T; catechins and their derivatives; chitin / chitosan derivatives. Furthermore, an extract having an antibacterial action can also be used as an antibacterial agent, and examples of such an extract include wikiweed, opac, chia, rose hip, force mitsu, orange, sagittarius, turmeric, gardenia, and chioji. Extracts such as sage, cypress, clove, eucalyptus, cypress, cedar.
酵素阻害剤は、 肌の角質を構成するコラーゲン、 エラスチン、 ヒアルロン 酸などを分解する分解酵素の阻害剤が主に用いられる。 酵素阻害剤としては、 例えば、 ビタミン C、 ビタミン E、 カテキンおよびその誘導体などが挙げら れる。 さらに、 酵素阻害剤として酵素阻害作用を有する抽出物なども用いら れ得、 このような抽出物としては、 ゥコン、 セィヨウノコギリソゥ、 ゼ-ァ オイ、 タイム、 ヤーコン、 ローズヒップ、 ローズマリー、 オトギリソゥ、 シ ャクャク、 ガラナ、 グアバ、 ァセンャク、 ブドウ、 コケモモ、 イチヨウ、 リ ンゴ、 トマト、 松樹皮などの抽出物が挙げられる。 Enzyme inhibitors are mainly inhibitors of degrading enzymes that degrade collagen, elastin, hyaluronic acid, etc., which make up the skin's skin. Examples of the enzyme inhibitor include vitamin C, vitamin E, catechin and derivatives thereof. In addition, extracts having an enzyme inhibitory action are also used as enzyme inhibitors. Such extracts include: turmeric, sorghum, zea oy, thyme, yacon, rosehip, rosemary, cypress, peony, guarana, guava, acacia, grape, bilberry, yew, Examples include extracts such as lingo, tomato and pine bark.
紫外線防止剤としては、 パラアミノ安息香酸、 サリチル酸ホモエステル、 Examples of UV inhibitors include paraaminobenzoic acid, salicylic acid homoester,
4ーメトキシケィ酸一 2—ェチルへキシル、 2—ヒドロキシー 4—メトキシ ベンゾフエノン、 2—ヒ ドロキシー 4ーメ トキシベンゾフエノンスノレホン酸 およびその塩、 4—tーブチルー 4 ' ーメトキシージベンゾィルメタン、 ゥ ロカユン酸、 酸化チタン、 酸化セリウム、 酸化亜鉛などが挙げられる。 4-Methoxyketic acid 1-ethylhexyl, 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone sulphonic acid and its salts, 4-tert-butyl-4'-methoxydibenzoylmethane, Examples include eurocauric acid, titanium oxide, cerium oxide, and zinc oxide.
上記食品原料を吸着または吸収させた湿潤組成物は、 そのままの形態で用 いてもよく、 例えば、 湿潤組成物を乾燥して粉末化し錠剤、 顆粒などの形態 に加工してもよく、 あるいは様々な加工品の原料として用いてもよい。  The wet composition in which the food material is adsorbed or absorbed may be used as it is. For example, the wet composition may be dried and powdered to be processed into tablets, granules, or various forms. You may use as a raw material of a processed product.
上記化粧品原料を吸着または吸収させた湿潤組成物は、 そのままの形態で 用いてもよく、 例えば、 医薬部外品、 化粧品、 トイレタリー用品などの形態 に加工され得る。 医薬部外品、 化粧品、 トイレタリー用品などとしては、 例 えば、 化粧クリーム、 轧液、 クリーム、 パック、 パッチ、 ヘアトニック、 へ ァクリーム、 シャンプー、 ヘアリンス、 トリートメント、 ボディシャンプー、 洗顔剤、 石鹼、 ファンデーション、 白粉、 口紅、 リップダロス、 頰紅、 アイ シャドー、 整髪料、 育毛剤、 水性軟膏、 油性軟膏、 歯磨剤、 マウスゥォッシ ュ、 シップ、 ゲルなどが挙げられる。 実施例  The wet composition in which the cosmetic raw material is adsorbed or absorbed may be used as it is, and may be processed into forms such as quasi-drugs, cosmetics and toiletries. Quasi-drugs, cosmetics, toiletries, etc., for example, cosmetic creams, liquids, creams, packs, patches, hair tonics, hair creams, shampoos, hair rinses, treatments, body shampoos, facial cleansers, sarcophagus, foundations White powder, lipstick, lip dallos, scarlet, eye shadow, hairdressing agent, hair restorer, aqueous ointment, oily ointment, dentifrice, mouthwash, ship, gel, etc. Example
以下、 実施例を挙げて本発明をより詳細に説明するが、 本発明は、 これら の実施例に限定されるものではない。  EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated in detail, this invention is not limited to these Examples.
(実施例 1 ) まず、 グルコース 25 g、 酵母エキス 5 g、 およびペプトン 3 gを精製水 100 OmLに溶解した。 次いで、 得られた水溶液の pHを 6に調整し、 ォ 一トクレーブで滅菌して培地を調製した。 次いで、 この培地 l O OmLに、 'ァセトパクターキシリナム （Acetobacter xylinum IF013693) を播種し、 2 5°Cで 14日間静置培養を行った。. 14日後、 培養液の表層に形成されたセ ルロース膜を回収した。 回収した膜を、 水でよく洗浄し、 1 200mLの 2 %水酸化ナトリウム溶液で 1時間煮沸した。 次いで、 水で中性になるまで 洗浄して、 網目構造を有するセルロース膜 1を得た。 (Example 1) First, 25 g of glucose, 5 g of yeast extract, and 3 g of peptone were dissolved in 100 OmL of purified water. Next, the pH of the obtained aqueous solution was adjusted to 6, and sterilized in an autoclave to prepare a medium. Subsequently, 'Acetobacter xylinum IF013693) was seeded on this medium l O OmL, and static culture was performed at 25 ° C for 14 days. After 14 days, the cellulose film formed on the surface of the culture was collected. The collected membrane was thoroughly washed with water and boiled with 1 200 mL of 2% sodium hydroxide solution for 1 hour. Next, the membrane was washed with water until neutral, and a cellulose membrane 1 having a network structure was obtained.
次いで、 このセルロース膜 1を水で飽和させて、 l gの正方形のシート状 に力ットした。 次いで、 カットして得られた水飽和状態のセルロース膜 1を 凍結乾燥し、 乾燥質量を測定したところ、 0. 04 gであった。 すなわち、 このセルロース膜 1は、 保持し得る水の量が乾燥質量の少なくとも 20倍で あることがわかった。 この水で飽和させたセルロース膜 1を、 支持体 1とし た。 .  Next, the cellulose membrane 1 was saturated with water and pressed into a 1 g square sheet. Next, the water-saturated cellulose membrane 1 obtained by cutting was freeze-dried and the dry mass was measured to find 0.04 g. That is, it was found that the amount of water that can be retained in the cellulose membrane 1 is at least 20 times the dry mass. The cellulose membrane 1 saturated with water was used as the support 1. .
(実施例 2) (Example 2)
市販の黒酢に、 グルコースを 1質量％となるように添カ卩して培地を調製し た。 次いで、 この培地 l O OmLに、 実施例 1で用いた微生物を播種し、 2 5 °Cで 1ヶ月間静置培養して、 セルロース膜を得た。 このセルロース膜は、 実施例 1で得られたセルロース膜 1に比べて臭いが少なかった。 このセル口 一ス膜を実施例 1と同様の手順で洗浄し、 網目構造を有するセルロース膜 2 を得た。  A medium was prepared by adding commercially available black vinegar to 1% by mass of glucose. Subsequently, the microorganism used in Example 1 was inoculated into this medium lO 2 OmL, and statically cultured at 25 ° C for 1 month to obtain a cellulose membrane. This cellulose membrane had less odor than the cellulose membrane 1 obtained in Example 1. This cell mouth membrane was washed in the same procedure as in Example 1 to obtain a cellulose membrane 2 having a network structure.
(実施例 3) (Example 3)
実施例 1で得られたセルロース膜 1を水で飽和させて、 1 gの正方形のシ 一ト状に力ットした。 次いで、 カットして得られた水飽和状態のセルロース 膜 1を、 除去される水分量を測定しながら圧搾した。 0 . 3 gの水が除去さ れた時点で圧搾を止めて、 カットして得られたセルロース膜 1の質量が、 飽 和湿質量の 7 0質量％となるように調製した。 次いで、 このカットして得ら' れたセルロース膜 1 (すなわち、 飽和湿質量の 7 0質量％の質量を有するセ ルロース膜 1 ) を精製水に一晚浸漬し、 質量を測定したところ、 0 . 9 8 g であった。 実施例 1に記載されているように、 セルロース膜 1の乾燥質量は、 0 . 0 4 gである。 したがって、 飽和湿質量の 7 0質量％の質量を有するセ ルロース膜 1は、 保持し得る水の量が乾燥質量の 2 0倍以上であることがわ かった。 この飽和湿質量の 7 0質量％の質量を有するセルロース膜 1を支持 体 2とした。 Cellulose membrane 1 obtained in Example 1 was saturated with water and pressed into a 1 g square sheet. Next, water-saturated cellulose obtained by cutting Membrane 1 was squeezed while measuring the amount of water removed. When 0.3 g of water was removed, the pressing was stopped, and the cellulose membrane 1 obtained by cutting was prepared so that the mass of the cellulose membrane 1 was 70% by mass of the saturated wet mass. Next, the cellulose membrane 1 obtained by this cutting (that is, cellulose membrane 1 having a mass of 70% by mass of saturated wet mass) was immersed in purified water for a while and its mass was measured. 9 8 g. As described in Example 1, the dry weight of the cellulose membrane 1 is 0.04 g. Therefore, it was found that the amount of water that can be retained in the cellulose membrane 1 having a mass of 70% by mass of the saturated wet mass is 20 times or more the dry mass. Cellulose membrane 1 having a mass of 70% by mass of the saturated wet mass was used as support 2.
(比較例 1 ) (Comparative Example 1)
実施例 1で得られたセルロース膜 1を水で飽和させて、 1 gの正方形のシ 一ト状に力ットした。 次いで、 カットして得られた水飽和状態のセルロース 膜 1を、 除去される水分量を測定しながら圧搾した。 0 . 9 gの水が除去さ れた時点で圧搾を止めて、 カットして得られたセルロース膜 1の質量が、 飽 和湿質量の 1 0質量％となるように調製した。 次いで、 このカットして得ら れたセルロース膜 1 (すなわち、 飽和湿質量の 1 0質量％の質量を有するセ ルロース膜 1 ) を精製水に一晚浸漬し、 質量を測定したところ、 0 . 3 gで あった。 実施例 1に記載されているように、 セルロース膜 1の乾燥質量は、 0 . 0 4 gである。 したがって、 飽和湿質量の 1 0質量％の質量を有するセ ルロース膜 1は、 保持し得る水の量が乾燥質量の 2 0倍以下であることがわ かった。 すなわち、 ー且、 多量の水を失ったセルロース膜は、 水を再吸収し 難くなることがわかった。 この飽和湿質量の 1 0質量％の質量を有するセル ロース膜 1を支持体 3とした。 (実施例 4 ：湿潤組成物の調製） Cellulose membrane 1 obtained in Example 1 was saturated with water and pressed into a 1 g square sheet. Next, the water-saturated cellulose membrane 1 obtained by cutting was compressed while measuring the amount of water removed. When 0.9 g of water was removed, the pressing was stopped, and the cellulose membrane 1 obtained by cutting was prepared so that the mass of the cellulose membrane 1 was 10% by mass of the saturated wet mass. Next, the cellulose membrane 1 obtained by this cutting (that is, a cellulose membrane 1 having a mass of 10% by mass of saturated wet mass) was immersed in purified water for a while and its mass was measured. It was 3 g. As described in Example 1, the dry weight of the cellulose membrane 1 is 0.04 g. Therefore, it was found that in the cellulose membrane 1 having a mass of 10% by mass of the saturated wet mass, the amount of water that can be retained is 20 times or less of the dry mass. That is, it was found that a cellulose membrane that has lost a large amount of water has difficulty in reabsorbing water. The cellulose membrane 1 having a mass of 10% by mass of the saturated wet mass was used as the support 3. (Example 4: Preparation of wet composition)
実施例 3および比較例 1で得られた支持体 2およぴ支持体 3を、 それぞれ 着色色素 （商品名 ：アンカレッド S P 5 0 0、 ヤエガキ醱酵技研株式会社 製） を 0 . 0 0 0 1質量％含有する水溶液 2 0 m Lに一晚浸漬した。 次いで、 支持体 2および支持体 3を水溶液から取り出し、 それぞれの支持体の表面を 精製水で洗浄して湿潤組成物 1 (支持体 2由来） および湿潤組成物 2 (支持 体 3由来） を得た。 湿潤組成物 1およぴ湿潤組成物 2の色の濃さを目視で比 較したところ、 湿潤組成物 1の方が、 湿潤糸且成物 2よりも色が濃かつた。 し たがって、 支持体 2の方が支持体 3よりも、 効率よく着色色素 （成分） を吸 着または吸収することがわかった。  The support 2 and the support 3 obtained in Example 3 and Comparative Example 1 were mixed with coloring pigments (trade name: Anchor Red SP 500, manufactured by Yaegaki Fermentation Co., Ltd.), respectively. It was immersed in 20 mL of an aqueous solution containing 1% by mass. Next, the support 2 and the support 3 are taken out from the aqueous solution, and the surfaces of the respective supports are washed with purified water to obtain a wet composition 1 (derived from the support 2) and a wet composition 2 (derived from the support 3). It was. As a result of visual comparison of the color strength of the wet composition 1 and the wet composition 2, the wet composition 1 was darker than the wet yarn 1 and the composition 2. Therefore, it was found that the support 2 adsorbs or absorbs the coloring pigment (component) more efficiently than the support 3.
(実施例 4 ) (Example 4)
実施例 1で得られた支持体 1を、 以下の成分を含有する水溶液に一晚浸漬 し、 湿潤組成物 3を得た （単位は質量 Z容量％) 。  The support 1 obtained in Example 1 was immersed in an aqueous solution containing the following components to obtain a wet composition 3 (unit: mass Z volume%).
多価アルコール （グリセリン)  Polyhydric alcohol (glycerin)
工タノ一/レ  Tanoichi / Le
防腐剤 レ、。ラベン）  Preservatives. Raven)
ヒァノレ口ン酸  Hyanolic acid
松樹皮抽出物  Pine bark extract
得られた湿潤組成物 3を皮膚に付着させると、 保湿感を感じた。 したがつ て、 湿潤組成物 3は、 優れた保湿作用を有していることがわかった。 産業上の利用可能性  When the obtained wet composition 3 was adhered to the skin, a moisturizing feeling was felt. Therefore, the wet composition 3 was found to have an excellent moisturizing action. Industrial applicability
本発明の支持体は、 機能性成分を含有する溶液または分散液に浸漬するこ とで、 溶液中または分散液中の機能性成分を効率よく吸着または吸収するこ とができる。 例えば、 機能性成分を含有する湿潤組成物を得ることができる。 このような支持体および湿潤組成物は、 優れた機能性および保湿性を有し、 医薬品、 食品、 医薬部外品、 化粧品、 トイレタリー製品などの基材として有 用である。 By immersing the support of the present invention in a solution or dispersion containing a functional component, the functional component in the solution or dispersion can be adsorbed or absorbed efficiently. For example, a wet composition containing a functional component can be obtained. Such a support and a wetting composition have excellent functionality and moisture retention, and are useful as base materials for pharmaceuticals, foods, quasi drugs, cosmetics, toiletries, and the like.

Claims

請求の範囲 The scope of the claims
1 . 網目構造を有するセルロースからなる支持体であって、 該支持体の乾燥 質量に対して、 2 0倍以上の質量の水を保持し得る、 支持体。 1. A support made of cellulose having a network structure, which can hold 20 times or more of water with respect to the dry weight of the support.
2 . 請求項 1に記載の支持体を、 所望の成分を含有する溶液または分散液に 浸漬して得られる、 湿潤組成物。 2. A wet composition obtained by immersing the support according to claim 1 in a solution or dispersion containing a desired component.
3 . 前記湿潤組成物がシート状である、 請求項 2に記載の湿潤組成物。 3. The wet composition of claim 2, wherein the wet composition is in sheet form.
4 . 前記成分が、 化粧品原料である、 請求項 2または 3に記載の湿潤組成物。 4. The wet composition according to claim 2 or 3, wherein the ingredient is a cosmetic raw material.
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JP2018100241A (en) * 2016-12-21 2018-06-28 ロレアル Composite sheet of biocellulose and sphaerotilus natans-derived microtubes
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WO2012096385A1 (en) * 2011-01-14 2012-07-19 学校法人 立教学院 Method for producing aerogel with pore-shaped portions, aerogel with pore-shaped portions, method for producing restored hydrogel, and restored hydrogel
JP2018100241A (en) * 2016-12-21 2018-06-28 ロレアル Composite sheet of biocellulose and sphaerotilus natans-derived microtubes
JP6988016B1 (en) * 2021-04-22 2022-01-05 佐藤製薬株式会社 Granzyme B inhibitor
JP2022166987A (en) * 2021-04-22 2022-11-04 佐藤製薬株式会社 Granzyme b inhibitors

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