Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
  • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
  • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
  • C07K16/249—Interferons
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
  • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
  • A61P17/06—Antipsoriatics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P3/00—Drugs for disorders of the metabolism
  • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
  • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/12—Antivirals
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/12—Antivirals
  • A61P31/14—Antivirals for RNA viruses
  • A61P31/18—Antivirals for RNA viruses for HIV
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P37/00—Drugs for immunological or allergic disorders
  • A61P37/02—Immunomodulators
  • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K39/00—Medicinal preparations containing antigens or antibodies
  • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
  • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
  • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
  • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
  • C07K2317/565—Complementarity determining region [CDR]
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
  • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding

Definitions

• the present invention relates to methods and compositions useful to diagnose and treat conditions correlated with an abnormal level of interferon- ⁇ (IFN ⁇ ) expression in a subject.
• IFN ⁇ interferon- ⁇
• Type I IFNs Human interferons
• Type I IFNs include six types (IFN- ⁇ , IFN- ⁇ , IFN- ⁇ , IFN-K, IFN- ⁇ , and IFN- ⁇ ).
• IFN- ⁇ , ⁇ , ⁇ , and ic act through an identical IFN receptor, IFNAR.
• IFN- ⁇ associates with a distinct receptor, IFNLR.
• the receptor for IFN- ⁇ is currently unknown.
• Type Il IFN consists of a single type (IFN - ⁇ ) and associates with the receptor IFNGR.
• Type I IFNs are strongly induced during viral infections, Type II IFN is induced primarily in response to immune and inflammatory stimuli, and thus IFN- ⁇ is frequently referred to as "immune IFN".
• the most studied of the numerous Type I IFNs include IFN- ⁇ , IFN- ⁇ , and IFN-co. Of these, IFN- ⁇ is the most complex, includes at least fifteen distinct protein subtypes exhibiting upwards of 75% sequence homology. Diaz (1995) Semin. Virol. 6: 143-149; Weissmann et al. (1986) Prog Nucl Acid Res MoI Biol 33:251; J Interferon Res (1993) 13:443-444; Roberts et al.
• IFN- ⁇ genes and their products show functional similarities. For example, they are induced by dsRNA or virus, and can interact with the same receptor, the IFN ⁇ / ⁇ receptor IFNAR. Mogensen, et al. (1999) J. Interferons and other Regulatory Cytokines, John Wiley & Sons. IFN ⁇ also inhibits apoptosis, promotes the survival and differentiation of antigen-activated T helper cells and promotes the maturation of functionally efficient monocyte -derived dendritic cells.
• IPCs interferon- ⁇ producing cells
• PBMCs peripheral blood mononuclear cells
• IFN- ⁇ has been implicated as a mediator of the pathology seen in several autoimmune diseases. Moreover, it can cause autoimmune disease development in patients treated with IFN- ⁇ for cancer and viral infections. Increased expression of IFN- ⁇ has been observed in the disease-localized tissues of patients with insulin-dependent diabetes mellitus (IDDM or type I diabetes), psoriasis, Crohn ' s disease, and celiac disease. Over expression of IFN- ⁇ has been observed in patients with systemic lupus erythematosus (SLR), IDDM and AIDS.
• SLR systemic lupus erythematosus
• IFN- ⁇ expression is observed in not only tissue lesions but circulating within the blood of afflicted individuals. Furthermore, the IFN- ⁇ serum levels tend to correlate with the clinical disease activity index. This is believed to stem from cyclical induction of normally quiescent monocytes into potent antigen-presenting dendritic cells (DCs) as triggered by upregulation of IFN- ⁇ production by plasmacytoid DCs (pDCs).
• DCs potent antigen-presenting dendritic cells
• SLE can be distinguished by "signatures" of unregulated genes involved in granulopoiesis and IFN induction; these signatures revert to normal upon high-dose infusion of glucocorticoids (U.S. patent application 1 1/228,586, the content of which is incorporated by reference hereto).
• SLE Systemic lupus erythematosus
• SLE include corticosteroids, nonsteroidal immune suppressants, antimalarials, and nonsteroidal anti-inflammatory drugs. These drugs abrogate the integrity of all immune effector responses rather than acting upon those specific to the pathogenesis of SLE.
• SLE represents an unmet medical need since these treatments are only partially effective with moderate to severe side-effects including bone thinning, weight gain, acne, anemia, sterility, rashes diarrhea, hair loss, and nausea. Furthermore, no new therapeutics for SLE have been approved in 40 years. 10007] SLE has recently been closely linked to unabated IFN ⁇ production. Shi et al. (1987) Br. J. Dermatol. 117(2): 155-159.
• H 7 Na is present at elevated levels in SLE serum (Crow et al. (2004) Curr. Opin. Rheumatol. 16(5):541-547) and plasmacytoid DCs (pDCs), the primary source of IFN ⁇ , accumulate in SLE skin.
• pDCs plasmacytoid DCs
• IFN ⁇ may act via the differentiation of monocytes into functional dendritic cells (DCs) which in turn mediates the etiopathogenesis of SLE. Pascual V. et al. (2003) Cui ⁇ . Opin. Rheumatol. 15(5):548-556.
• a proposed approach for the treatment of SLE is neutralization of IFN ⁇ (see Banchereau et al., PCT/US02/00343, the contents of which is incorporated by reference).
• 2003/0166228 A l discloses a monoclonal antibody (designated 9F3) that was derived from immunization of mice with leukocyte IFN ⁇ (which includes all of the IFN ⁇ protein subtypes).
• the 9F3 MAb binds and neutralizes the anti-viral activity of the proteins encoded by seven human IFN ⁇ gene subtypes 1 , 2, 4, 5, 8, 10 and 2 L (which encode IFN ⁇ protein subtypes D, A, 4, G, B2, C and I ⁇ respectively), without neutralizing the antiviral activity of human IFN ⁇ .
• IFN ⁇ is a multi-functional mediator of the immune response and has beneficial antiviral activity
• complete inhibition or significant down-regulation of all IFN ⁇ subtypes is not an optimal therapeutic approach.
• This invention satisfies this need and provides related advantages as well.
• the invention provides monoclonal antibodies and derivatives thereof that neutralize specific IFN ⁇ subtypes.
• the antibodies of the invention are useful in the amelioration and treatment of conditions associated with increased expression of IFN ⁇ , such as SLE, psoriasis, type I diabetes, AIDS, Graft versus Host Disease and other autoimmune diseases.
• the invention includes an antibody that selectively neutralizes a bioactivity of at least two interferon alpha ("IFN ⁇ " ) proiein subtypes such as subtypes A, 2, B2, C.
• IFN ⁇ interferon alpha
• the antibody preferably binds essentially the same, or the same, or competes with an antibody that binds essentially the same, or the same, IFN ⁇ epitope as the anti-IFN ⁇ antibody produced by the hybridoma having ATCC Accession No. PTA-7778.
• the antibody is a monoclonal antibody.
• the antibody does not significantly neutralize IFN ⁇ protein subtype D nor 1.
• the monoclonal antibody is ACO-2.
• the antibodies of the invention inactivate the ability of serum isolated from systemic lupus erythematosus (SLE) patients to stimulate the differentiation of monocytes into dendritic cells.
• IFN ⁇ monoclonal anti-interferon alpha
• the antibodies, derivatives or fragments thereof of the invention can be mouse, rat, human, or from other mammals, or fragments or humanized or chimeric fo ⁇ ns thereof.
• the antibodies of the invention include mouse monoclonal antibodies, e.g., ACO-2, as well as humanized forms, chimeric forms, or fragments thereof.
• the invention further provides antibodies that bind to essentially the same IFN ⁇ epitope as murine monoclonal antibody ACO-2.
• the invention further provides host cells, hybridomas, compositions, pharmaceutical compostions and kits comprising the antibodies of the invention.
• the antibodies, derivatives or fragments thereof of the invention have use in the treatment of diseases or conditions associated with overexpression of IFN ⁇ , including but not limited to SLE, psoriasis, AIDS, type I diabetes and autoimmune thyroiditis, and for the production of medicaments to treat such diseases and conditions.
• the antibodies of the invention can also be used to distinguish or to purify various IFN ⁇ subtypes.
• the invention also includes host cells and hybridoma cell lines that produce antibodies having the above-noted specificities, e.g., specific binding to one or more interferon alpha ("IFN ⁇ ") protein subtypes (A, 2, B2, C, F, G, H2, I, Jl, K, 4a, 4b and WA).
• IFN ⁇ interferon alpha
• the antibodies and/or hybridoma cell lines can be combined with a carrier, such as a pharmaceutically acceptable earner, for use in diagnostic and therapeutic methods.
• the antibodies are useful to detect specific IFN ⁇ subtypes and to diagnose, prognose, treat and/or ameliorate symptoms of IFN ⁇ related disorders. Examples of such conditions include, but are not limited to SLE, psoriasis, type T diabetes, Graft versus Host (GVH) Disease, AIDS, autoimmune thyroiditis and other autoimmune disorders.
• the antibodies of the invention are also useful to neutralize and/or isolate these IFN ⁇ subtypes in vitro or in vivo.
• Figure 1 shows a schematic diagram of the Reporter Gene (RG) assay and the Cytopathic Effect Inhibition Assay (CPE).
• the filled circles in the CPE assay diagram represent live, intact cells.
• the unfilled circles represent dead cells, killed by viral infection.
• the filled circles and cells as well as the "+" in the RG assay diagram represent luciferase expression, while the unfilled circles and cells represent the lack of luciferase expression.
• Figure 2 shows a flow chart of the IFN ⁇ MAb development scheme.
• Figure 3 shows neutralization of complex IFN sources by ACO-I , 2, 3, 4, and 5.
• Figure 5 shows the results of a multiplex analysis of monoclonal antibodies ACO-
• Figure 6 shows the results of solid-phase binding assay of IFN ⁇ subtypes by monoclonal antibodies ACO-I, ⁇ CO-2, ⁇ CO-3, ⁇ CO-4, ACO-5, and ACO-6.
• Figure 7 shows the neutralization of SLE patient serum samples SLE-43 (a), SLE-
• Controls include serum alone, media only (-), and a pan- neutralizing polyclonal antibody (pAb, rabbit and anti-human IFN ⁇ , PBL). Values represent the mean of triplicates.
• Figure 8A-C shows the cross-reactivity of ACO-I (A), ACO-2 (B). and ACO-3
• Figure 9 shows the cDN ⁇ and amino acid sequence of the heavy chain from ACO- 1.
• the DNA sequence encoding the Vn 1, V] j2 and VH3 CDRS are shown in italics, while the corresponding amino acid sequences are underlined.
• Figure 10 shows the cDNA and amino acid sequence of the light chain from ACO-
• antibody refers to all classes and subclasses of intact immunoglobulins.
• the term “antibody” also covers monoclonal antibodies, antibody fragments and antibody fragment clones.
• Antibody fragments include a portion of an intact antibody that contains the antigen binding or variable region of the intact antibody. Examples of antibody fragments include Fab, Fab', F(ab')2, and Fv fragments; single-chain antibody molecules, multispecific antibodies formed from antibody fragments; a Fd fragment includes the VH and CH, domains; a Fv fragment includes the VL and VII domains of a single arm of an antibody, a dAb fragment (Ward et al.
• Single-chain Fv or “scFv” antibody fragments include the VH and VL domains of antibody, wherein these domains are present in a single polypeptide chain.
• the scFv polypeptide further includes a polypeptide linker between the VFI and VL domains, which enables the scFv to form the desired structure for antigen binding.
• any of the above -noted antibody fragments may be obtained using conventional techniques known to those of skill in the art, and the fragments are screened for binding specificity and neutralization activity in the same manner as are intact antibodies.
• the antibodies can be isolated from any suitable biological source, e.g., murine, rabbit, rat, human, sheep, canine, etc.
• “Naturally occurring” or “native” antibodies are heterotetrameric glycoproteins, typically having a molecular weight of approximately 150 - 200 kD.
• the heterotetramer includes two identical light (L) chains and two identical heavy (H) chains. Each light chain is covalently bonded to a heavy chain by a disulfide bond.
• antibody derivative refers to encompass molecules that bind an epitope and which are modifications or derivatives of a native monoclonal antibody of this invention. Derivatives include, but are not limited to, for example, bispecific, multispecific, heterospecific, trispecific, tetraspecific, multispecific antibodies, chimeric, recombinant and humanized.
• antibody variant refers to antibodies produced in a species other than a mouse or an isotype of an antibody selected from the antibodies designated ACO-I through ACO-6 and ACO-8.
• antibody variant also includes antibodies containing post-translational modifications to the linear polypeptide sequence of the antibody or fragment. It further encompasses fully human antibodies.
• the term "monoclonal antibody”, as used herein, refers to an antibody (including antibody fragments) obtained from a population of substantially homogeneous antibodies, i.e., the individual antibodies in the population are identical except for possible naturally occurring mutations that may be present in minor amounts, which are also part of the present invention so long as they exhibit the desired biological activity.
• Monoclonal antibodies are highly specific and directed against a single epitope.
• Monoclonal antibodies may be synthesized by a hybridoma culture, in a bio-reactor, as an ascites or made by recombinant methods, such as in vitro translation, in bacteria, yeast, plants, insect and/or animal cells.
• the modifier "monoclonal ' ' indicates the character of the antibody as being obtained from a substantially homogeneous population of antibodies, and is not to be construed as requiring production of the antibody by any particular method.
• the monoclonal antibodies to be used in accordance with the present invention may be made by the hybridoma method first described by Kohler et al. (1975) Nature, 256:495, or may be made by recombinant DNA methods (see, e.g., U.S. 4,816,567).
• “Monoclonal antibodies” includes, but is not limited to human monoclonal antibodies, humanized monoclonal antibodies, recombinant human antibodies, clones of antigen-recognition and binding-site containing antibody fragments (Fv clones) isolated from phage antibody libraries and derivatives thereof. (See Clackson, et al. (1991) Nature, 352:624-628; and Marks, et al. (1991 ) J MoI Biol 222:581-597).
• Monoclonal antibodies also include "chimeric" antibodies (immunoglobulins) in which, e.g., a portion of the heavy and/or light chain is identical with or homologous to corresponding sequences in antibodies derived from a particular species or belonging to a particular antibody class or subclass, while the remainder of the chain(s). or portions thereof, is identical with or homologous to corresponding sequences in antibodies derived from another species or belonging to another antibody class or subclass, as well as fragments of such antibodies, so long as they exhibit the desired biological activity (U.S. 4,816,567; Morrison, et al., (1984) Proc Natl Acad Sci USA, 81 :6851-6855).
• the term '"human monoclonal antibody refers to antibodies displaying a single binding specificity that have variable and constant regions derived from human germline immunoglobulin sequences.
• human antibody refers to antibodies having variable and constant regions derived from human germline immunoglobulin sequences.
• the human antibodies of the invention may include amino acid residues not encoded by human germline immunoglobulin sequences (e.g., mutations introduced by random or site-specific mutagenesis in vitro or by somatic mutation in vivo).
• human antibody as used herein, is not intended to include antibodies in which CDR sequences derived from the germline of another mammalian species, such as a mouse, have been grafted onto human framework sequences.
• human antibody refers to an antibody in which substantially every part of the protein (e.g., CDR, framework, CL, CH domains (e.g., CH 1 , CH 2 , CII 3 ), hinge, (VL, VH)) is substantially non-immunogenic in humans, with only minor sequence changes or variations.
• antibodies designated primate monkey, baboon, chimpanzee, etc.
• rodent mouse, rat, rabbit, guinea pig, hamster, and the like
• other mammals designate such species, sub-genus, genus, sub-family, family specific antibodies.
• chimeric antibodies may also include any combination of the above.
• a human antibody is distinct from a chimeric or humanized antibody.
• a human antibody may be produced by a non-human animal or prokaryotic or eukaryotic cell that is capable of expressing functionally rearranged human immunoglobulin (e.g., heavy chain and/or light chain) genes. Further, when a human antibody is a single chain antibody, it may also include a linker peptide that is not found in native human antibodies.
• an Fv fragment may also include a linker peptide, such as two to about eight glycine or other amino acid residues, which connects the variable region of the heavy chain and the variable region of the light chain.
• linker peptides are considered to be of human origin.
• a human antibody is "derived from” a particular germline sequence if the antibody is obtained from a system using human immunoglobulin sequences, e.g., by immunizing a transgenic mouse carrying human immunoglobulin genes or by screening a human immunoglobulin gene library.
• a human antibody that is "derived from” a human germline immunoglobulin sequence can be identified as such by comparing the amino acid sequence of the human antibody to the amino acid sequence of human germline immunoglobulins.
• a selected human antibody typically is at least 90% identical in amino acids sequence to an amino acid sequence encoded by a human germline immunoglobulin gene and contains amino acid residues that identify the human antibody as being human when compared to the germline immunoglobulin amino acid sequences of other species (e.g., mouse or rat germline sequences).
• a human antibody may be at least 95%, or even at least 96%, 97%, 98%, or 99% identical in amino acid sequence to the amino acid sequence encoded by the germline immunoglobulin gene.
• a human antibody derived from a particular human germline sequence will display no more than 10 amino acid differences from the amino acid sequence encoded by the human germline immunoglobulin gene.
• the human antibody may display no more than 5, or even no more than 4, 3, 2, or 1 amino acid difference from the amino acid sequence encoded by the ge ⁇ nline immunoglobulin gene.
• humanized refers to the use of portions of a non-human (e.g., mouse or rat) antibodies that are used on a human immunoglobulin backbone to make chimeric immunoglobulins, immunoglobulin chains or fragments thereof (such as Fv, Fab, Fab', F(ab')2 or other antigen-binding subsequences of antibodies) that have sequences derived from non-human immunoglobulin.
• a non-human e.g., mouse or rat
• immunoglobulin chains or fragments thereof such as Fv, Fab, Fab', F(ab')2 or other antigen-binding subsequences of antibodies
• humanized antibodies are human immunoglobulins (recipient antibody) in which residues from part or all of one or more complementarity-determining regions (CDRs) of the recipient antibody are replaced by residues from one or more CDRs of a non-human species (donor antibody) such as mouse, rat or rabbit having the desired specificity, affinity, and capacity.
• CDRs complementarity-determining regions
• donor antibody such as mouse, rat or rabbit having the desired specificity, affinity, and capacity.
• Fv framework region (FR) residues of the human immunoglobulin are replaced by corresponding non- human residues, or vice-versa, that is, human immunoglobulin portions may be grafted onto the non-human immunoglobulin regions that determine antigen specificity.
• humanized antibodies may include residues that are found neither in the recipient antibody, nor in the imported CDR or framework sequences.
• the humanized antibody will include substantially all of at least one, and typically both, variable domains (light and heavy), in which all or substantially all of the CDR regions correspond to those of a non-human immunoglobulin and all or substantially all of the FR regions are those of a human immunoglobulin sequence.
• the humanized antibody may also include at least a portion of an immunoglobulin constant region (Fc), typically that of a human immunoglobulin.
• recombinant human antibody includes all human antibodies that are prepared, expressed, created or isolated by recombinant method, such as antibodies isolated from an animal (e.g., a mouse) that is transgenic or transchromosomal for human immunoglobulin genes or a hybridoma prepared therefrom, antibodies isolated from a host cell transformed to express the antibody, e.g., from a cell transfected to express the antibody (commonly a plasmacytoma), antibodies isolated from a recombinant, combinatorial human antibody library, and antibodies prepared, expressed, created or isotated by any other method that may involve splicing of human immunoglobulin gene sequences to other DNA sequences.
• an animal e.g., a mouse
• transgenic or transchromosomal for human immunoglobulin genes or a hybridoma prepared therefrom antibodies isolated from a host cell transformed to express the antibody, e.g., from a cell transfected to express the antibody (commonly a plasmacytoma)
• Such recombinant human antibodies have variable and constant regions derived from human germline immunoglobulin sequences. In certain embodiments, however, such recombinant human antibodies can be subjected to in vitro mutagenesis (or, when an animal transgenic for human Ig sequences is used, in vivo somatic mutagenesis) and thus the amino acid sequences of the VH and VL regions of the recombinant antibodies are sequences that, while derived from and related to human germline VH and VL sequences, may not naturally exist within the human antibody germline repertoire in vivo.
• the terms "antigen-binding site" or "binding portion”, as used herein, refer to the part of an immunoglobulin molecule that participates in antigen binding.
• the antigen binding site is formed by amino acid residues of the N-terminal three variable (“V") regions of the heavy (“H”) chain and three variable regions of light (“L”) chains.
• V variable
• L variable regions of light
• CDRs hypervariable regions
• Framework regions refer to amino acid sequences that are found naturally between, and adjacent to, hypervariable regions in immunoglobulins.
• the three hypervariable regions of a light chain (V L 1, V L 2 and V 1 3) and the three hypervariable regions of a heavy chain (V H 1 , V H 2 and V H 3) are disposed relative to each other in three dimensional space to form an antigen-binding surface.
• the antigen- binding surface is complementary to the three-dimensional surface of a bound antigen, and the three hypervariable regions of each of the heavy and light chains are referred to as "complementarity-determining regions" or "CDRs.”
• bioactivity refers to the ability of one or more IFN ⁇ subtypes (or IFN ⁇ ) to activate the MxA promoter (and interferon-inducible promoter) or to exert an antiviral effect.
• the EC 50 and percent neutralization for an antibody of an IFN ⁇ bioactivity can vary depending on the assay conditions and the type of IFN ⁇ bioactivity measured. For consistency, specific types of bioaclivities (i.e.. activation of the M x A promoter and antiviral activity) and assay conditions (i.e., the "RG assay” and the "CFE assay”) are used. The RG assay can be performed using the conditions described herein.
• Percent neutralization of activation of the M x A promoter is determined as described in the Examples (see Example 3), using RGmax IFN amounts and 2 micrograms per mL antibody.
• the antiviral (CPE) assay can be performed according to the methods described in the examples.
• bispecific molecule refers to any agent, e.g., a protein, peptide, or protein or peptide complex, which has two different binding specificities.
• multispecific molecule or “heterospecific molecule” is intended to include any agent, e.g. a protein, peptide, or protein or peptide complex, which has more than two different binding specificities.
• An antibody binds "essentially the same epitope" as a reference antibody when the two antibodies recognize identical or sterically overlapping epitopes.
• Antibody binding may be measured or determined by standard antibody-antigen assays, for example, competition assays, saturation assays, or standard immunoassays such as ElJSA or RIA.
• competition assays which can be configured in a number of different formats using either labeled antigen or labeled antibody.
• the antigen is immobilized on a 96-well plate, and the ability of unlabeled antibodies to block the binding of labeled antibodies is measured using radioactive or enzyme labels.
• composition refers to a combination of active agent and another carrier, e.g., compound or composition, inert (for example, a detectable agent or label) or active, such as an adjuvant, diluent, binder, stabilizer, buffers, salts, lipophilic solvents, preservative, adjuvant or the like.
• carrier e.g., compound or composition
• inert for example, a detectable agent or label
• active such as an adjuvant, diluent, binder, stabilizer, buffers, salts, lipophilic solvents, preservative, adjuvant or the like.
• Carriers also include pharmaceutical excipicnts and additives proteins, peptides, amino acids, lipids, and carbohydrates (e.g., sugars, including monosaccharides, di-, tri-, tetra-, and oligosaccharides; derivatized sugars such as alditols, aldonic acids, estcrified sugars and the like; and polysaccharides or sugar polymers), which can be present singly or in combination, including alone or in combination 1-99.99% by weight or volume.
• Exemplary protein excipients include serum albumin such as human serum albumin (HSA), recombinant human albumin (rHA), gelatin, casein, and the like.
• amino acid/antibody components which can also function in a buffering capacity, include, e.g., alanine, glycine, arginine, betaine, histidine, glutamic acid, aspartic acid, cysteine, lysine, leucine, isoleucine, valine, methionine, phenylalanine, aspartame, and the like.
• Carbohydrate excipients are also intended within the scope of this invention, examples of which include but are not limited to monosaccharides such as fructose, maltose, galactose, glucose, D-mannose, sorbose, and the like; disaccharides, such as lactose, sucrose, trehalose, ccllobiose, and the like; polysaccharides, such as raff ⁇ nose, mclczitose, maltodextrins. dextrans, starches, and the like; and alditols.
• monosaccharides such as fructose, maltose, galactose, glucose, D-mannose, sorbose, and the like
• disaccharides such as lactose, sucrose, trehalose, ccllobiose, and the like
• polysaccharides such as raff ⁇ nose, mclczitose, maltodext
• the term carrier further includes a buffer or a pH adjusting agent; typically, the buffer is a salt prepared from an organic acid or base.
• Representative buffers include organic acid salts such as salts of citric acid, ascorbic acid, gluconic acid, carbonic acid, tartaric acid, succinic acid, acetic acid, or phthalic acid; Tris, tromethamine hydrochloride, or phosphate buffers.
• Additional carriers include polymeric excipients/additives such as polyvinylpyrrolidones, ficolls (a polymeric sugar), dextrates (e.g., cyclodextrins, such as 2-hydroxypropyl-. quadrature. -cyclodextrin), polyethylene glycols, flavoring agents, antimicrobial agents, sweeteners, antioxidants, antistatic agents, surfactants (e.g., polysorbates such as "TWEEN 20 " and "TWEEN 80"), lipids (e.g., phospholipids, fatty acids), steroids (e.g., cholesterol), and chelating agents (e.g., EDTA).
• polymeric excipients/additives such as polyvinylpyrrolidones, ficolls (a polymeric sugar), dextrates (e.g., cyclodextrins, such as 2-hydroxypropyl-. quadrature. -cyclodextrin), polyethylene glycols
• control' ' refers to is an alternative subject or sample used in a study for comparison purpose. ⁇ control can be "positive” or “negative”.
• effective amount refers to is an amount sufficient to effect beneficial or desired results. ⁇ n effective amount can be administered in one or more administrations, applications or dosages.
• epitope refers to a site on an antigen, or an antigen fragment, recognized by an antibody or an antigen receptor.
• ⁇ T cell epitope is a short peptide derived from a protein antigen that is presented by the appropriate Major Histocompatibility Compatibility (MHC) protein.
• MHC Major Histocompatibility Compatibility
• B-ccll epitopes are generally antigenic determinants recognized by B cells and are commonly portions of a three- dimensional surface that are recognized by an antibody, which may include sequential or conformational determinants, as will be known to the skilled artisan.
• Antigenic determinant regions and predicted epitopes for the IFN ⁇ antibodies may be identified by any method for determining antigenic determinant regions, such as standard mapping and characterization techniques, further refinement of which can be accomplished by application of any suitable technique (references to "epitope mapping” techniques, "epitope identification” techniques, and the like, herein, should be understood as describing techniques applicable to the identification and/or refinement of epitopes).
• an epitope for ACO-2 antibody may be determined by epitope "ibotprinting" using chemical modification of the exposed amiiies/carboxyls in the IFN ⁇ protein.
• HXMS hydrogen-deuterium exchange detected by mass spectrometry
• a hydrogen/deuterium exchange of receptor and ligand protein amide protons is instituted, followed by peptide-anfigen binding, and back exchange of receptor and ligand protein amide protons.
• the backbone amide groups participating in protein binding are protected from back exchange and therefore remain deuterated.
• Relevant regions can be identified at this point by peptic proteolysis, fast microbore high-performance liquid chromatography separation, and/or electrospray ionization mass spectrometry. See, e.g., Ehring H, Analytical Biochemistry, Vol. 267 (2) pp.
• epitope mapping examples include, but are not limited to, nuclear magnetic resonance (NMR) epitope mapping, mass spectrometry methods, protease digestion techniques, and various phage display techniques. Examples of such techniques are described for example in U.S. Patent Publication No. 2007/0065447, the entirety of which is incorporated herein by reference. Numerous other methods are also known in the art for epitope mapping, such as those described for example in O.M.R. Westwood and F.C. Hay, EPITOPE MAPPING: A PRACTICAL APPROACH (Oxford Univ. Press, 2000).
• IgG antibodies can be cleaved into three fragments by papain digestion. Two of these fragments are typically identical antigen-binding fragments, called "Fab" fragments, each with a single antigen-binding site, and a residual "Fc" fragment.
• the Fab fragment contains the light chain and the amino-terminal half of the heavy chain held together by an interchain disulfide bond.
• the Fc fragment consists of the carboxyterminal halves of the two heavy chains disulfide -bonded to each other by the residual hinge region.
• Fab' fragments differ from Fab fragments by the addition of a few residues at the carboxy terminus of the heavy chain CHl domain including one or more cysteines from the antibody hinge region.
• Fab'-SH is the designation for Fab' in which the cysteine residue(s) of the constant domains bear a free thiol group.
• Fv refers to the minimum antibody fragment that includes a complete antigen-recognition and binding site.
• this region includes a dimer of one heavy- and one light-chain variable domain in non-covalent association.
• one heavy- and one light-chain variable domain may be linked covalently by a flexible peptide linker such that the light and heavy chains can associate in a "dimeric" structure analogous to that in a two-chain Fv species.
• heteroantibodies refers to two or more antibodies, antibody binding fragments (e.g., Fab), derivatives thereof, or antigen binding regions linked together that have at least two have different antigen specificities.
• IFNcT Interferon Alpha
• IFNcT Interferon Alpha
• IFN ⁇ B2 is sometimes also referred to as IFN ⁇ B, and is not to be confused with IFN ⁇ .
• Natural IFN ⁇ from leukocytes (leukocyte IFN), as well as these recombinant human IFN ⁇ protein subtypes are available from PBL Biomedical Labs, Piscataway, NJ (interferonsource.com). Natural IFN ⁇ is a complex mixture of IFN ⁇ subtypes. IFN ⁇ has not been detected in the natural IFN ⁇ preparations used herein.
• IFN ⁇ -producing cell refers to a specialized leukocyte that is responsible for IFN ⁇ production which is broadly induced by double stranded RNA (ds)RNA, viruses, bacteria, protozoa, certain cell lines and unmethylated CpG-DNA. Ronnblom and Aim (2004) J. Exp. Med. 194(12):F59-F63.
• ds double stranded RNA
• IFN ⁇ related condition or disease' refers to abnormal and deleterious diseases or pre-clinical disease states that have been linked with elevated levels of IFN ⁇ in a patient's serum. Examples of such include, but are not limited to SLE,
• GVHD graft versus Host Disease
• type 1 diabetes type 1 diabetes
• HIV human immunodeficiency virus
• HIV human immunodeficiency virus
• autoimmune thyroiditis psoriasis and lupus.
• Methods for determining the level of IFN ⁇ are known in the art and described herein.
• immuno response refers to the antigen-specific responses of lymphocytes to foreign substances. Any substance that can elicit an immune response is considered to be “immunogenic” and is referred to as an "immunogen". All immunogens are antigens, however, not all antigens are immunogenic. An immune response can be humoral (via antibody activity) or cell-mediated (via T cell activation).
• immunological binding refers to the non-covalent interactions of the type which occur between an immunoglobulin molecule and an antigen for which the immunoglobulin is specific.
• the strength, or affinity of immunological binding interactions can be expressed in terms of the dissociation constant (K d ) of the interaction, wherein a smaller K d represents a greater affinity.
• Immunological binding properties of selected polypeptides can be quantified using methods well known in the art.
• One such method entails measuring the rates ot antigen-binding site/antigen complex formation and dissociation, w r herein those rates depend on the concentrations of the complex partners, the affinity of the interaction, and on geometric parameters that equally influence the rate in both directions.
• both the "on rate constant” (K 0n ) and the “off rate constant” (K off ) may be determined by calculation of the concentrations and the actual rates of association and dissociation as are well known in the art.
• the ratio of K off /K 0n enables cancellation of all parameters not related to affinity, and is thus equal to the dissociation constant K d . See, e.g., Coligan, et al., CURRENT PROTOCOLS IN IMMUNOLOGY, Wiley, NY (1999).
• isolated refers to an antibody that has been identified and separated and/or recovered from a component of its natural environment. Contaminant components of its natural environment are materials that may interfere with diagnostic or therapeutic uses for the antibody, and may include enzymes, hormones, and other proteinaceous or nonproteinaceous solutes.
• the antibody will be purified (1) to greater than 95% by weight of antibody as determined by the Lowry method, and most preferably more than 99% by weight, (2) to a degree sufficient to obtain at least 15 residues of N-terminal or internal amino acid sequence by use of a spinning cup sequenator, or (3) to homogeneity by SDS-PAGE under reducing or nonreducing conditions using Coomassie blue or, preferably, silver stain.
• Isolated antibody includes the antibody in situ within recombinant cells since at least one component of the antibody's natural environment will not be present. Ordinarily, however, isolated antibody may be prepared by at least one purification step. Monoclonal antibodies and variants and derivatives thereof are considered isolated antibodies.
• isotype refers to the antibody class based on the amino acid sequence of the constant domain of their heavy chains.
• immunoglobulins There are five major isotypes of immunoglobulins: IgA, IgD, IgE, IgG, and IgM, and several of these can be further divided into subclasses, e.g., IgGl, IgG2, IgG3, IgG4, IgAl, and IgA2.
• “Lupus” refers to several diseases or disorders.
• Systemic lupus erythematosus (SLE) is the form of the disease that can affect many parts of the body. The symptoms of SLE may be mild or serious, and are reviewed herein.
• Discoid lupus erythematosus is a chronic skin disorder in which a red, raised rash appears on the face, scalp, or elsewhere. The raised areas may become thick and scaly and may cause scarring. The rash may last for days or years and may recur. A small percentage of people with discoid lupus have or develop SLE later.
• Subacute cutaneous lupus erythematosus refers to skin lesions that appear on parts of the body exposed to sun.
• Drug-induced lupus is a form of lupus caused by certain medications. Symptoms are similar to those of SLE (arthritis, rash, fever, and chest pain) and they typically go away completely when the drug is stopped. The kidneys and brain are rarely involved.
• Neonatal lupus is a rare disease that can occur in newborn babies of women with SEE, Sjogren's syndrome, or no disease at all, and may be caused by autoantibodies in the mother's blood called anti-Ro (SSA) and anti-La (SSB). At birth, the babies typically have a skin rash, liver problems, and low blood counts.
• compositions may also include stabilizers and preservatives and any of the above noted carriers with the additional provisio that they be acceptable for use in vivo.
• carriers e.g., a phosphate buffered saline solution, water, and emulsions, such as an oil/water or water/oil emulsion, and various types of wetting agents.
• the compositions may also include stabilizers and preservatives and any of the above noted carriers with the additional provisio that they be acceptable for use in vivo.
• carriers, stabilizers and adjuvants see Martin REMINGTON'S PHARM. SCL, 18th Ed., Mack Publ. Co., Easton, PA (1995), and in the "PHYSICIAN'S DESK REFERENCE", 58th ed., Medical Economics, Montvale, NJ. (2004).
• the terms, “selectively neutralizes” and “selectively neutralizing”, as used herein, refer to an isolated and purified antibody (such as, but not limited to a monoclonal antibody) that neutralizes selectively at least 40% of a bioactivity of one or more "IFN ⁇ " protein subtypes, but does not significantly neutralize at least one bioactivity of another IFN ⁇ protein subtype, wherein the bioactivity is activation of the MxA promoter or antiviral activity. Since the different subtypes of IFN ⁇ vary in function, it is advantageous to selectively neutralize specific forms of IFN ⁇ to control specific functions.
• the one or more antibodies of the present invention are specific for IFN ⁇ , but are also "selective' " for one or more subtypes and not others.
• one or more antigenic epitopes on IFN ⁇ that do not significantly cross-react with antigenic epitopes on, e.g., the D subtype have been identified, isolated, characterized and purified, as disclosed herein.
• the phrase "does not significantly neutralize”, as used herein, refers to an antibody that neutralizes less than 40% of the bioactivity of a specified IFN ⁇ subtype (or of IFN ⁇ ), wherein the bioactivity is measured by the MxA reporter gene assay (RG assay) or cylopathic effect assay (CPE assay) in accordance with the conditions described herein.
• an antibody of the invention does not significantly neutralize more than 35%, 30%, 25%, 20%, 15%, 10%, 5%, 2% or even not more than 1% of the bioactivity of the specified IFN ⁇ subtype.
• subject refers to a vertebrate, preferably a mammal, more preferably a human. Mammals include, but are not limited to, rodents, simians, humans, farm animals, horses, dogs and cats.
• ⁇ desirable MAb candidate against IFN- ⁇ would require specific characteristics, including: (i) ability to react against most or all of the human IFN- ⁇ subtypes involved in the etiology of SLE; (ii) ability to block the biological activities of such IFN- ⁇ subtypes, (iii) inability to block cither IFN- ⁇ or the IFN ⁇ R; and/or (iv) high affinity.
• Neutralizing IFN- ⁇ rather than the IFNAR may also provide a safer and more specific therapeutic since this approach would not affect the antiviral effects of the IFN- ⁇ signaling pathway, which uses the same receptor as IFN- ⁇ .
• the invention also provides a series of monoclonal antibodies (MAbs) capable of neutralizing human IFN- ⁇ .
• two of these anti-IFN- ⁇ MAbs are capable of blocking the bioactivity of thirteen recombinant IFN- ⁇ subtypes as well as two complex mixtures of IFN produced upon viral infection.
• the invention provides seven MAbs that variably neutralize human IFN- ⁇ , of which three significantly neutralize up to thirteen recombinant IFN- ⁇ subtypes and complex IFN- ⁇ mixtures (both commercially- available leukocyte IFN and supernatants produced upon infection of PBMCs with flu virus).
• Two of the M ⁇ bs, ACO-I and ACO-2 also consistently block the bioactivity of serum from SLE patients that exhibit IFN- ⁇ signatures by microarray analysis.
• ACO-I and ACO-2 do not significantly neutralize the bioactivity of IFN ⁇ protein subytpes D and 1, but do neutralize the IFN ⁇ bioactivity of SLE serum, these subtypes are unlikely to be involved significantly in the etiology of SLE. Accordingly, it is desirable to treat SLE using antibodies, such as humanized or non-antigenic (e.g., deimmunized) variants of ACO-I and ACO-2, which block the bioactivity of IFN ⁇ subtypes associated with SLE, but do not block the bioactivities of IFN ⁇ protein subytpes (D and 1) that are not significantly associated with SLE.
• antibodies such as humanized or non-antigenic (e.g., deimmunized) variants of ACO-I and ACO-2, which block the bioactivity of IFN ⁇ subtypes associated with SLE, but do not block the bioactivities of IFN ⁇ protein subytpes (D and 1) that are not significantly associated with SLE.
• the invention also provides an antibody that selectively neutralizes a bioactivity of at least two interferon alpha ("IFN ⁇ ") protein subtypes selected from the group consisting of protein subtypes A. 2, B2, C, F, G, 112, I, Jl, K, 4a, 4b and WA. but does not significantly neutralize at least one bioactivity of IFN ⁇ protein subtype D; wherein the bioactivity is, e.g,. activation of the MxA promoter and/or antiviral activity.
• IFN ⁇ interferon alpha
• the invention provides a method for treating a disease or condition associated with abno ⁇ nal expression of at least one interferon alpha ("IFN ⁇ ") protein subtype selected from protein subtypes A, 2, B2, C, F, G, H2, I, J l, K, 4a, 4b and WA without neutralizing IFN ⁇ protein subtype D antiviral activity, in a subject, including administering to the subject an effective amount of one or more of the antibodies described herein.
• IFN ⁇ interferon alpha
• antibodies include, but are not limited to, the antibodies designated ACO-I, ACO-2, ACO-3, ACO-4, ACO-5, ACO-6 and antibodies that recognize the same or essentially the same IFN ⁇ epitope, or antibodies that compete with an antibody that recognizes essentially the same, or the same IFN ⁇ epitope as any of the foregoing antibodies.
• the antibodies are monoclonal antibodies.
• the ATCC deposit numbers of hybridoma cell lines that produce these monoclonal antibodies are listed hereinbelow. Accordingly, the invention further provides hybridoma cell lines expressing the ACO-I , ACO-2, ACO-3, ACO-4, ⁇ CO-5, ACO-6, and ACO-8 antibodies.
• the antibody binds essentially the same IFN ⁇ epitope as the anti-lFN ⁇ antibody produced by the hybridoma having ATCC Accession No. PTA-7778. In another aspect, the antibody binds to the same IFN ⁇ epitope as the anti-IFN ⁇ antibody produced by the hybridoma having ATCC Accession No. PTA-7778. In a further aspect, the antibody competes with an antibody that binds with essentially the same or the same IFN ⁇ epitope as the anti-IFN ⁇ antibody produced by the hybridoma having ATCC Accession No. PTA-7778.
• the invention provides an antibody that binds essentially the same IFN ⁇ epitope as an antibody selected from the group consisting of ACO-I, ACO-2, ACO-3, ACO-4, ACO-5 and ACO-6.
• the invention provides an antibody that competes with essentially the same IFN ⁇ epitope as an antibody selected from the group consisting of ACO-I , ACO-2, ACO-3, ACO-4, ACO-5, and ACO-6.
• the invention provides an antibody that binds the same epitope as an antibody selected from the group consisting of ACO-I , ⁇ CO-2, ACO-3, ACO-4, ACO-5, and ACO-6.
• the invention provides an antibody that competes with an antibody that binds the same IFN(X epitope as an antibody selected from the group consisting of ACO-I , ACO-2, ACO-3, ACO-4, ACO-5, and ACO-6.
• the invention further provides cell lines, e.g.. a hybridoma, which expresses such antibodies.
• the invention provides an antibody that neutralizes a bioactivity of at least 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13 or 14 IFN ⁇ protein subtypes selected from protein subtypes A, 2, B2, C, F, G, H2, I, Jl , K, 4a, 4b and WA, but does not neutralize at least one bioactivity of IFN ⁇ protein subtype D; wherein the bioactivity is activation of the MxA promoter or antiviral activity.
• the invention further provides hybridoma cell lines expressing such antibodies.
• Monoclonal antibodies are provided that do not neutralize at least one bioactivity of IFN ⁇ protein subtype 1, wherein the bioactivity is activation of the MxA promoter and/or antiviral activity.
• the invention provides a monoclonal antibody that selectively neutralizes a bioactivity the IFN ⁇ protein subtypes A, 2, B2, C, F, G, H2, I, K, 4a, 4b and WA, but does not significantly neutralize the bioactivity of IFN ⁇ protein subtypes D and 1, wherein the bioactivity is activation of the MxA promoter and/or antiviral activity.
• Other embodiments include ACO- 1 and ACO-2.
• the invention provides a monoclonal antibody that selectively neutralizes the bioactivity the IFN ⁇ protein subtypes A, 2, B2, C, I, K and 4a, but does not significantly neutralize the bioactivity of IFN ⁇ protein subtypes D, F, G, 4b and 1 ; wherein the bioactivity is activation of the MxA promoter and/or antiviral activity.
• a monoclonal antibody that selectively neutralizes the bioactivity the IFN ⁇ protein subtypes A, 2, B2, C, I, K and 4a, but does not significantly neutralize the bioactivity of IFN ⁇ protein subtypes D, F, G, 4b and 1 ; wherein the bioactivity is activation of the MxA promoter and/or antiviral activity.
• One such embodiment is the ACO-3 antibody made by the ACO-3 cell and derivatives thereof.
• the invention provides an antibody that selectively neutralizes the bioactivity the IFN ⁇ protein subtypes A, 2, B2, and C, but does not significantly neutralize the bioactivity of IFN ⁇ protein subtypes D, 4b, and 1, wherein the bioactivity is activation of the MxA promoter and/or antiviral activity.
• One such embodiment is the ACO-4 antibody and derivatives thereof made by the ACO-4 cell and derivatives thereof.
• the antibody of this invention selectively neutralizes a bioactivity IFN ⁇ 4a, but does not selectively neutralize IFN ⁇ 4b, wherein the bioactivity is activation of the MxA promoter.
• these antibodies are the antibodies designated ACO-3 and ⁇ CO-4, and derivatives thereof, made by, e.g., the ACO-3 and ACO-4 cells and derivatives thereof, respectively.
• the invention provides a monoclonal antibody that selectively neutralizes the bioactivity the IFNu protein subtypes A, 2, G, I, K, WA and 1, but does not significantly neutralize the bioactivity of IFN ⁇ protein subtypes B2 and D, wherein the bioactivity is activation of the MxA promoter and/or antiviral activity.
• One such embodiment is the ACO-5 antibody and derivatives thereof, made by, e.g., the ACO-5 cell and derivatives thereof.
• the invention provides a monoclonal antibody that selectively neutralizes the bioactivity the IFN ⁇ protein subtypes 2 and C, but does not neutralize the bioactivity of IFN ⁇ protein subtypes A, B2, C. D, F and 1, wherein the bioactivity is activation of the MxA promoter.
• a monoclonal antibody that selectively neutralizes the bioactivity the IFN ⁇ protein subtypes 2 and C, but does not neutralize the bioactivity of IFN ⁇ protein subtypes A, B2, C. D, F and 1, wherein the bioactivity is activation of the MxA promoter.
• An example is the ACO-6 antibody and derivatives thereof made by the ACO-6 cell and derivatives thereof.
• the invention provides a monoclonal antibody that selectively neutralizes the bioactivity the IFN ⁇ protein subtypes A, 2, B2, D, F, I, 4a, 4b, and 1, but does not significantly neutralize the bioactivity of IFN ⁇ protein subtypes C, H2, K and WA, wherein the bioactivity is activation of the MxA promoter.
• An example is the ACO-8 antibody and derivatives thereof made by the ACO-8 cell and derivatives thereof.
• the invention provides a monoclonal an ti -interferon alpha ("IFN ⁇ ”) antibody produced by the hybridoma having ATCC Accession No. PTA-7778.
• IFN ⁇ ti -interferon alpha
• the invention provides an antibody that selectively neutralizes a bioactivity of at least two interferon alpha ("IFN ⁇ ") protein subtypes with a half maximal effective concentration (EC 50 ) of less than about 350 ng/ml, more preferably less than about 300 ng/ml, for subtypes selected from the group consisting of protein subtypes A, 2, B2, C, F, G. 112, 1. Jl , 4a, 4b and WA and/or less than about 400 ng/ml, more preferably less than about 375 ng/ml for subtype K.
• Half maximal effective concentrations may be determined using any method available, preferably using the RCi bioassay as described herein.
• the antibody molecules of the present invention may have a high binding affinity for IFN ⁇ protein subtypes (e.g. nanomolar binding). Affinity may be measured using any suitable method known in the art, including the Biacore assay as described in the examples herein. Preferably affinity is measured using recombinant IFNa-A as described in the examples herein.
• An antibody molecule according to the present invention may have a binding affinity for IFN ⁇ - ⁇ of less than about 5xl(T 9 M, or an antibody molecule according to the present invention may have a binding affinity for IFNa-A of less than 1.5xlO ⁇ 9 M.
• an antibody molecule of the present invention has a binding affinity of between about 9x10 "9 M and about 4x10 "10 M. In another embodiment, an antibody molecule of the present invention may have a binding affinity of between about 5x10 " ° M and about 1.5x10 "9 M.
• the monoclonal antibodies of the invention also include a humanized antibody, a human antibody, a chimeric antibody, an antibody fragment, such as an Fab fragment, an F(ab')2 fragment, an Fab' fragment or any other fragment(s) known to the skilled artisan. In one example, the antibody is a humanized chimeric antibody.
• the invention provides a method of producing a hybridoma cell line by, for example, immunizing a mammal with a composition including recombinant IFN ⁇ subtypes A, B2 and F; fusing splenocytes from the mammal to a myeloma cell line to produce hybridomas; and identifying a hybridoma cell line that produces a monoclonal antibody that selectively neutralizes one or more IFN ⁇ protein subtypes selected from the group consisting of 2, C, G, I, Jl, K, 4a, 4b and WA and 1, but does not selectively neutralize IFN ⁇ protein subtype D.
• the term “neutralizes”, as used herein, refers to the ability of an antibody to inhibit one or more biological activities of an IFN ⁇ protein subtype by at least 40% as measured by the RG, CPE or monocyte differentiation assays defined herein.
• the antibody neutralizes at least 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 97%, 98%, 99% or 100% of a biological activity of an IFN ⁇ protein subtype.
• IFN ⁇ biological activities include transcriptional activation of the MxA promoter (see, e.g., "RG " ' assay, infra), antiviral activity (e.g., cytopathic effect assay ("CPF").
• the antibody neutralizes less than 35%, or less than 35%, or less than 30%, or less than 25%. or less than 20%, or less than 15%, or less than 10%, or less than 8%, or less than 5%, or less than 3%, or even less than 1 % of the bioaclivity.
• the antibody of the invention may be antibody variants, derivatives or fragments.
• the antibodies are isolated, ⁇ n another aspect, the antibodies are combined with a suitable carrier.
• the antibodies can be isolated from any species, mouse, rat, simian, or recombinantly produced. Examples of mouse monoclonal antibodies are the antibodies designated ACO-I , ACO-2, ⁇ CO-3, ⁇ CO-4, ⁇ CO-5, ACO-6 and ACO-8.
• Also provided by this invention are the hybridoma cell lines that produce these monoclonal antibodies, alone in combination with a carrier or in culture.
• polypeptides that include an antibody, variant, derivative or fragment thereof, including but not limited to immunoglobulin chains and CDRs.
• the polypeptides preferably bind, inhibit and/or neutralize LFN ⁇ as described above, with the same or similar affinity and/or ability.
• the present invention further provides an anti-idiotypic antibody reactive with any of the antibodies ⁇ CO-1 through ACO-6 or ACO-8.
• An anti-idiotype antibody is an antibody made against the unique determinants of a single antibody. Anti-idiotype antibodies are useful for detecting bound antibodies in immunoassays and other applications.
• An antiidiotype antibody of the invention can include or be derived from any mammal, such as but not limited to a human, a mouse, a rabbit, a rat. a rodent, a primate, and the like.
• One or more of the above antibodies can be further combined with a carrier, a pharmaceutically acceptable carrier or medical device that is suitable for use of the antibody or related composition in diagnostic or therapeutic methods.
• the carrier can be a liquid phase carrier or solid phase earner, e.g., bead, gel or carrier molecule such as a liposome.
• the composition can optionally further include at least one further compound, protein or composition.
• An additional example of "carriers" includes therapeutically active agents such as another peptide or protein (e.g., an Fab' fragment, a J-chain. another antibody, a toxin and the like).
• an anti-EFN ⁇ antibody of this invention can be functionally linked (e.g., by chemical coupling, genetic fusion, noncovaleiit association or otherwise) to one or more other molecular entities, such as another antibody (e.g., to produce a bispccific or a multispecific antibody), a cytotoxin, a cellular ligand or an antigen.
• this invention encompasses a large variety of antibody conjugates, bi- and multispecific molecules, and fusion proteins, whether or not they target the same epitope as the antibodies of this invention.
• Additional examples of carriers are organic molecules (also termed modifying agents) or activating agents that may be attached covalently, directly or indirectly, to an antibody of this invention.
• Attachment of the molecule can improve pharmacokinetic properties (e.g., increased in vivo serum half-life).
• organic molecules include, but are not limited to a hydrophilic polymeric group, a fatty acid group or a fatty acid ester group.
• fatty acid refers to, e.g., mono-carboxylic acids and di- carboxylic acids.
• hydrophilic polymeric group refers to an organic polymer that is, e.g., more soluble in water than in octane.
• Hydrophilic polymers suitable for modifying antibodies of the invention can be linear or branched and include, for example, polyalkane glycols (e.g., polyethylene glycol (PEG), monomethoxy-polyethylene glycol (mPEG), polypropylene glycol (PPG) and the like), carbohydrates (e.g., dextran, cellulose, oligosaccharides, polysaccharides and the like), polymers of hydrophilic amino acids (e.g., polylysine, polyargininc, polyaspartate and the like), polyalkane oxides (e.g., polyethylene oxide, polypropylene oxide and the like) and polyvinyl pyrolidone.
• polyalkane glycols e.g., polyethylene glycol (PEG), monomethoxy-polyethylene glycol (mPEG), polypropylene glycol (PPG) and the like
• carbohydrates e.g., dextran, cellulose, oligosaccharides, polys
• a suitable hydrophilic polymer for use with the antibody of the invention may have a molecular weight of,e.g., about 800 to about 150,000 Daltons as a separate molecular entity.
• the hydrophilic polymeric group can be substituted with one to about six alkyl, fatty acid or fatty acid ester groups.
• Hydrophilic polymers that are substituted with a fatty acid or fatty acid ester group can be prepared by employing suitable methods. For example, a polymer including an amine group can be coupled to a carboxylate of the fatty acid or fatty acid ester, and an activated carboxylate (e.g., activated with N, N-carbonyl diimidazole) on a fatty acid or fatty acid ester can be coupled to a hydroxy! group on a polymer.
• an activated carboxylate e.g., activated with N, N-carbonyl diimidazole
• Fatty acids and fatty acid esters suitable for modifying antibodies of the invention can be saturated or can contain one or more units of unsaturation. Examples of such include, but are not limited to, n-dodecanoate, n-tetradecanoate, n-octadecanoate, n-eicosanoate, n- docosanoate, n-triacontanoate.
• Suitable fatty acid esters include mono-esters of dicarboxylic acids that include a linear or branched lower alkyl group.
• the lower alkyl group can include from one to about twelve, preferably one to about six, carbon atoms.
• the present invention provides a transgenic nonhuman animal, such as a transgenic mouse (also referred to herein as a "Human MAb mouse"), which expresses a fully human monoclonal antibody that neutralizes at least one IFN ⁇ protein subtype similar to an antibody of this invention as defined above.
• the transgenic nonbuman animal is a transgenic mouse having a genome including a human heavy chain transgene and a human light chain transgene encoding all or a portion of an anti- alpha V antibody of the invention.
• the transgenic nonhuman animal can be immunized with a purified or enriched preparation of IFN ⁇ protein subtypes A, B and F.
• ⁇ n example of a transgenic nonhuman animal may be, e.g., a transgenic mouse that is capable of producing multiple isotypes of human monoclonal antibodies to IFN ⁇ (e.g., IgG, IgA and/or IgM) by undergoing V-D-J recombination and isotype switching, lsotype switching may occur by, e.g., classical or non-classical isotype switching.
• IFN ⁇ e.g., IgG, IgA and/or IgM
• the invention provides isolated cells derived or isolated from a transgenic nonhuman animal as described above, e.g.. a transgenic mouse, which express human antibodies.
• the isolated B-cells can then be immortalized by fusion to an immortalized cell to provide a source (e.g., a hybridoma) of human antibodies.
• a source e.g., a hybridoma
• the present invention further provides at least one antibody method or composition, for diagnosing at least one IFN ⁇ related condition in a cell, tissue, organ, animal or patient and/or, prior to, subsequent to, or during a related condition, as known in the art and/or as described herein. They are also used to prognose or monitor disease progression.
• compositions comprising at least one anti-IFN ⁇ antibody of this invention, variant, derivative or fragment thereof, suitable for administration in an effective amount to modulate or ameliorate IFN ⁇ -associated symptoms or treat at least one IFN ⁇ related condition in a cell, tissue, organ, animal or patient and/or, prior to, subsequent to, or during a related condition, as known in the art and/or as described herein.
• the compositions include, for example, pharmaceutical and diagnostic compositions/kits, including a pharmaceutically acceptable carrier and at least one anti-IFN ⁇ antibody of this invention, variant, derivative or fragment thereof.
• the composition can further include additional antibodies or therapeutic agents which in combination, provide multiple therapies tailored to provide the maximum therapeutic benefit.
• compositions of this invention can be co-administered with other therapeutic and cytotoxic agents, whether or not linked to them or administered in the same dosing. They can be coadministered simultaneously with such agents (e.g., in a single composition or separately) or can be administered before or after administration of such agents.
• agents can include corticosteroids, nonsteroidal immune suppressants, antimalarials, and nonsteroidal anti-inflammatory drugs.
• the compositions can be combined with alternative therapies such as administration of corticosteroids, nonsteroidal immune suppressants, antimalarials, and nonsteroidal anti-inflammatory drugs.
• the invention provides methods for selectively neutralizing a bioactivity of at least two interferon alpha (''IFNa") protein subtypes selected from the group consisting of protein subtypes A, 2, B2, C, F, G, 112. I, Jl, K, 4a, 4b and WA, without selectively neutralizing at least one bioactivity of IFN ⁇ protein subtype D; wherein the bioactivity is activation of the MxA promoter or antiviral activity.
• the method requires contacting the sample suspected of containing the subtype with a monoclonal antibody that selectively neutralizes the subtype. Examples of such antibodies include, but are not limited to the antibodies designated ACO-I , ACO-2, ACO-3, ACO-4, ACO-5 and ACO-6.
• this specification discloses methods for ameliorating the symptoms associated with abnormal expression of at least one interferon alpha ("LFN ⁇ ”) protein subtype selected from the group consisting of protein subtypes A, 2, B2, C, F, G, H2, L K, 4a, 4b and WA without binding IFN ⁇ protein subtypes D and 1 in a subject, by administering to the subject an effective amount of an antibody that selectively neutralizes the subtype. Examples of such arc provided above.
• the invention provides methods for treating a disease or condition which results in abnormal expression of at least one interferon alpha ("IFN ⁇ ”) protein subtype selected from the group consisting of protein subtypes A. 2, B2, C, F, G, H2.
• the immunization method includes administering to a mammal a composition consisting essentially of IFN ⁇ subtypes A, B2 and F, a pharmaceutically acceptable carrier, and optionally, one or more adjuvants.
• immunization raises antibodies that neutralize IFN ⁇ protein subtypes A, B2 and F. but not IFN ⁇ protein subtypes D and 1.
• the method and composition neutralizes IFNa 4a and not IFN ⁇ 4b (e.g., ACO-3).
• the antibodies and compositions of this invention are administered or delivered to patients (e.g., human subjects) at therapeutically effective dosages to neutralize selected IFN ⁇ subtypes. Additionally, administration or delivery of effective amounts of antibodies or compositions of this invention can be use to treat or ameliorate the symptoms of an IFN ⁇ related condition such as SEE, diabetes, psoriasis or ATDS, in a subject using any suitable route of administration for antibody-based clinical products. Many are known in the art, such as injection or infusion. [00096] Dosages Forms.
• a dosage unit for use of the antibodies of the present invention may be a single compound or mixtures thereof with other compounds. The compounds may be mixed together, form ionic or even covalent bonds.
• One or more of the antibodies of the present invention may be administered in oral, intravenous (bolus or infusion), intraperitoneal, subcutaneous, or intramuscular form, all using dosage forms well known to those of ordinary skill in the pharmaceutical arts.
• different dosage forms e.g., tablets, capsules, pills, powders, granules, elixirs, tinctures, suspensions, syrups, and emulsions may be used to provide the antibody(ies) of the present invention to a patient in need of therapy that includes the neutralization of certain IFN ⁇ subtypes, as described herein.
• the antibodies are generally hydrophobic but they may be administered as any one of known salt forms.
• Antibodies are typically administered in admixture with suitable pharmaceutical salts, buffers, diluents, extenders, excipients and/or carriers (collectively referred to herein as a pharmaceutically acceptable carrier or cairiei mateiials) selected based on the intended form of administration and as consistent with conventional pharmaceutical practices.
• a pharmaceutically acceptable carrier or cairiei mateiials selected based on the intended form of administration and as consistent with conventional pharmaceutical practices.
• the antibodies may be formulated to provide, e.g., maximum and/or consistent dosing for the particular form for oral, rectal, topical, intravenous injection or parenteral administration.
• antibodies e.g., humanized forms of ACO- 1 , ACO-2, ACO-3, ACO-4, ACO-5, ACO-6 and ACO-8
• the carrier may be solid or liquid, depending on the type and/or location of administration selected.
• he antibodies of the present invention may be administered in the form of liposome delivery systems, e.g., small unilamellar vesicles, large unilamallar vesicles, and multilamellar vesicles, whether charged or uncharged.
• liposomes may include one or more: phospholipids (e.g., cholesterol), stcarylamine and/or phosphatidylcholines, mixtures thereof, and the like.
• the antibodies may also be coupled to one or more soluble, biodegradable, bioacceptable polymers as drug carriers or as a prodrug.
• polymers may include: polyvinylpyrrolidone, pyran copolymer, polyhydroxylpropylmethacrylamide-phenol, polyhydroxyethylasparta-midephenol, or polyefhyleneoxide-polylysine substituted with palmitoyl residues, mixtures thereof, and the like.
• biodegradable polymers for use with the present invention include: polylactic acid, polyglycolic acid, copolymers of polylactic and polyglycolic acid, polyepsilon caprolactone, polyhydroxy butyric acid, polyorthoesters, polyacetals. polydihydropyrans, polycyanoacylates, and crosslinked or amphipathic block copolymers of hydrogels, mixtures thereof, and the like.
• the antibodies may also be delivered as an intranasal form via use of a suitable intranasal vehicle.
• a suitable intranasal vehicle for direct delivery to the nasal passages, sinuses, mouth, throat, esophagous, tachea, lungs and alveoli, the antibodies may also be delivered as an intranasal form via use of a suitable intranasal vehicle.
• the antibodies may be delivered using lotions, creams, oils, elixirs, serums, transdermal skin patches and the like, as are well known to those of ordinary skill in that art.
• Parenteral and intravenous forms may also include pharmaceutically acceptable salts and/or minerals and other materials to make them compatible with the type of injection or delivery system chosen, e.g., a buffered, isotonic solution.
• useful pharmaceutical dosage forms for administration of antibodies may include the following forms.
• a parenteral composition suitable for administration by injection is prepared by stirring 1.5% by weight of active ingredient in deionized water and mixed with, e.g., up to 10% by volume propylene glycol and water.
• the solution is made isotonic with sodium chloride and sterilized using, e.g., ultrafiltration.
• Sterile injectable solutions are prepared by incorporating the active compounds in the required amount in the appropriate solvent with various of the other ingredients enumerated above, as required, followed by filtered sterilization.
• dispersions are prepared by incorporating the various sterilized active ingredients into a sterile vehicle that has a basic dispersion medium.
• useful methods for the preparation of a dry-powder include, vacuum-drying, spray-freezing, vaccum drying in the presence of heat, and freeze-drying techniques, which yield a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof.
• the antibodies of the present invention may be delivered as micro or nanoparticles in an injectable form or via pulmonary or other delivery. [000103] Suspensions.
• an aqueous suspension may be prepaied for administration so that each 5 ml has, e.g., 0.001 - 1,000 mg of finely divided active ingredient, 200 mg of sodium carboxymethyl cellulose, 5 mg of sodium benzoate, 1.0 g of sorbitol solution, and saline to 0.01, 0.1, 1, 5 or 10 ml.
• the effective dose of antibody may include amounts yielding upon reconstitution, if in a wet/dry system, concentrations from about l .O ⁇ g/ml to about 1000 mg/ml, although lower and higher concentrations are operable and are dependent on the intended delivery vehicle, e.g., solution formulations will differ from transdermal patch, pulmonary, transmucosal, or osmotic or micro pump methods.
• Formulations including an antibody of this invention are provided herein.
• the formulations of the present invention can be prepared by a process that includes mixing at least one antibody of this invention and a preservative selected from the group consisting of phenol, m-crcsol, p-cresol, o-cresol, chlorocresol, benzyl alcohol, alkylparaben, (methyl, ethyl, propyl, butyl and the like), benzalkonium chloride, benzethonium chloride, sodium dehydroacetate and thimerosal or mixtures thereof in an aqueous diluent.
• a preservative selected from the group consisting of phenol, m-crcsol, p-cresol, o-cresol, chlorocresol, benzyl alcohol, alkylparaben, (methyl, ethyl, propyl, butyl and the like), benzalkonium chloride, benzethonium chloride, sodium dehydro
• a measured amount of at least one antibody in buffered solution is combined with the desired preservative in a buffered solution in quantities sufficient to provide the antibody and preservative at the desired concentrations.
• Variations of this process would be recognized by one of ordinary skill in the art, e.g., the order the components are added, whether additional additives are used, the temperature and pH at which the formulation is prepared, are all factors that can be optimized for the concentration and method of administration used.
• the formulation may include one or more preservative or stabilizer such as phenol, m-cresol, p-cresol, o-cresol, chlorocresol, benzyl alcohol, phenylmercuric nitrite, phenoxyethanol, formaldehyde, chlorob ⁇ tanol, magnesium chloride (e.g., hexahydrate), alkylparaben (methyl, ethyl, propyl, butyl and the like), benzalkonium chloride, benzethonium chloride, sodium dehydroacetate and thimerosal, or mixtures thereof in an aqueous diluent.
• preservative or stabilizer such as phenol, m-cresol, p-cresol, o-cresol, chlorocresol, benzyl alcohol, phenylmercuric nitrite, phenoxyethanol, formaldehyde, chlorob ⁇ tanol, magnesium chloride (e.g., hexahydrate
• Any suitable concentration or mixture can be used as known in the art, such as 0.001-5%, or any range or value therein, such as, but not limited to 0.001 , 0.003, 0.005, 0.009, 0.01, 0.02, 0.03, 0.05, 0.09, 0.1, 0.2, 0.3, O.4., 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.1, 3.2, 3.3, 3.4,
• Non- limiting examples include, no preservative(s), or preservatives such as, e.g., 0.1-2% m-cresol (e.g., 0.2, 0.3. 0.4, 0.5, 0.9, 1.0%), 0.1-3% benzyl alcohol (e.g., 0.5, 0.9, 1.1., 1.5, 1.9, 2.0.
• preservatives such as, e.g., 0.1-2% m-cresol (e.g., 0.2, 0.3. 0.4, 0.5, 0.9, 1.0%), 0.1-3% benzyl alcohol (e.g., 0.5, 0.9, 1.1., 1.5, 1.9, 2.0.
• lhimerosal e.g., 0.005, 0.01
• phenol e.g., 0.05, 0.25, 0.28, 0.5, 0.9, 1.0%)
• alkylparaben(s) e.g., 0.00075, 0.0009, 0.001, 0.002, 0.005, 0.0075, 0.009, 0.01 , 0.02.
• compositions and formulations can be provided to patients as clear solutions or as dual vials including a vial of lyophilized antibody that is reconstituted with a second vial containing the aqueous diluent.
• a single solution vial or dual vial requiring reconstitution can be reused multiple times and can suffice for a single or multiple cycles of patient treatment and thus provides a more convenient treatment regimen than currently available.
• Recognized devices including these single vial systems include those pen-injector devices for delivery of a solution such as BD Pens, BD Autojectore, HumajectTM NovoPenTM, B-D ' J ' MPen, AutoPenTM, and OptiPenTM, GenotropinPenTM, Genotronorm PenTM, Humatro PenTM, Reco-PenTM, Roferon PenTM, BiojectorTM. IjectTM, J-tip Needle-Free InjectorTM, IntrajectTM, Medi-Ject, e.g., as made or developed by Becton Dickensen (Franklin Lakes, NJ.
• the invention provides an article of manufacture, including packaging material and at least one vial including a solution of at least antibody as of this invention with the prescribed buffers and/or preservatives, optionally in an aqueous diluent, wherein the packaging material includes a label that indicates that such solution can be held over a period of 1, 2, 3, 4, 5, 6, 9, 12, 18, 20, 24, 30. 36, 40, 48, 54, 60, 66, 72 hours or greater.
• the invention further includes an article of manufacture, including packaging material, a first vial including at least one lyophilized antibody of this invention and a second vial including an aqueous diluent of prescribed buffer or preservative, wherein the packaging material includes a label that instructs a patient to reconstitute the antibody in the aqueous diluent to form a solution that can be held over a period of twenty-four hours or greater.
• kits useful, for example, for the treatment of a disease conditions
• the kit may include one or more containers that include the pharmaceutical composition that may be provided as is, diluted or resuspended into a therapeutically effective amount of antibodies.
• Such kits may further include, if desired, one or more of various conventional pharmaceutical kit components, such as. for example, containers with one or more pharmaceutically acceptable carriers, liquids, additional containers, etc., as will be readily apparent to those skilled in the art.
• Printed instructions either as inserts or as labels, indicating quantities of the components to be administered, guidelines for administration, and/or guidelines for mixing the components, may also be included in the kit. It should be understood that although the specified materials and conditions are important in practicing the invention, unspecified materials and conditions are not excluded so long as they do not prevent the benefits of the invention from being realized.
• Antibodies The antibodies of this invention include monoclonal antibodies.
• Antibodies can be IFN ⁇ -neutralizing functional fragments, antibody derivatives or antibody variants. They can be chimeric, humanized, or totally human.
• a functional fragment of an antibody includes, but is not limited to, Fab, Fab', Fab 2 , Fab' 2 , and single chain variable regions.
• Antibodies can be produced in cell culture, e.g., in bacteria, yeast, plants or plant cells, insects or insect cells, eukaryotic cell, or in various animals, including but not limited to cows, rabbits, goats, mice, rats, hamsters, guinea pigs, sheep, dogs, cats, monkeys, chimpanzees, apes, etc.
• Antibodies can be tested for specificity of binding by comparing binding to appropriate antigen to binding to irrelevant antigen or antigen mixture under a given set of conditions. If the antibody binds to the appropriate antigen at least 2, 5, 7, and even 10 times more than to irrelevant antigen or antigen mixture then it is considered to be specific.
• Specific assays e.g., ELISA, for determining specificity are described infra.
• the antibodies also are characterized by their ability to neutralize one or more biological activity of an IFN ⁇ protein subtype, such as, but not limited to, transcriptional activation of the MxA promoter or of another promoter that is inducible by IFN ⁇ , antiviral activity, ability of SLE serum to cause differentiation of monocytes into dendritic cells.
• an IFN ⁇ protein subtype such as, but not limited to, transcriptional activation of the MxA promoter or of another promoter that is inducible by IFN ⁇ , antiviral activity, ability of SLE serum to cause differentiation of monocytes into dendritic cells.
• a hybridoma is produced by fusing a suitable immortal cell line (e.g., a myeloma cell line such as, but not limited to, Sp2/0, Sp2/0-AG 14, NSO, NSl , NS2, AE-I , L.5, >243, P3X63 ⁇ g8.653, Sp2 SA3, Sp2 MAI, Sp2 SSl, Sp2 S ⁇ 5, U397, MLA 144, ACT IV, MOLT4, DA-I, JURKAT, WEHI, K-562.
• a suitable immortal cell line e.g., a myeloma cell line such as, but not limited to, Sp2/0, Sp2/0-AG 14, NSO, NSl , NS2, AE-I , L.5, >243, P3X63 ⁇ g8.653, Sp2 SA3, Sp2 MAI, Sp2 SSl, Sp2 S ⁇ 5, U397, MLA 144, ACT IV, MOLT4, DA-I, JURKAT
• COS COS, RAJI, NlH 3T3, HL-60, MLA 144.
• Antibody producing cells can also be obtained from the peripheral blood or, preferably the spleen or lymph nodes, of humans or other suitable animals that have been immunized with the antigen of interest. Any other suitable host cell can also be used for expressing-heterologous or endogenous nucleic acid encoding an antibody, specified fragment or variant thereof, of the present invention.
• the fused cells (hybridomas) or recombinant cells can be isolated using selective culture conditions or other suitable known methods, and cloned by limiting dilution or cell sorting, or other known methods.
• Suitable methods of producing or isolating antibodies of the requisite specificity can be used, including, but not limited to. methods that select recombinant antibody from a peptide or protein library (e.g., but not limited to, a bacteriophage, ribosome, oligonucleotide, RNA, cDNA, or the like, display library; e.g., as available from various commercial vendors such as Cambridge Antibody Technologies (Cambridgeshire, UK), MorphoSys (Martinsreid/Planegg, Del.), Biovation (Aberdeen, Scotland. UK), Biolnvent (Lund, Sweden), and Antitopc (Cambridge, UK) using methods known in the art. See U.S.
• Antibody variants of the present invention can also be prepared using delivering a polynucleotide encoding an antibody of this invention to a suitable host such as to provide transgenic animals or mammals, such as goats, cows, horses, sheep, and the like, that produce such antibodies in their milk. These methods are known in the art and are described for example in U.S. 5,827,690: 5,849,992; 4,873.316; 5,849,992; 5,994,616; 5,565,362: and 5.304.489.
• antibody variant refers to post-translational modification to linear polypeptide sequence of the antibody or fragment.
• antibody variant refers to post-translational modification to linear polypeptide sequence of the antibody or fragment.
• U.S. 6,602,684 Bl describes a method for the generation of modified glycol-forms of antibodies, including whole antibody molecules, antibody fragments, or fusion proteins that include a region equivalent to the Fc region of an immunoglobulin, having enhanced Fc- mediated cellular toxicity, and glycoproteins so generated.
• Antibody variants also can be prepared by delivering a polynucleotide of this invention to provide transgenic plants and cultured plant cells (e.g.. but not limited to tobacco, maize, and duckweed) that produce such antibodies, specified portions or variants in the plant parts or in cells cultured therefrom.
• transgenic plants and cultured plant cells e.g.. but not limited to tobacco, maize, and duckweed
• plant cells e.g., but not limited to tobacco, maize, and duckweed
• Transgenic maize have been used to express mammalian proteins at commercial production levels, with biological activities equivalent to those produced in other recombinant systems or purified from natural sources. See, e.g., Hood et al., Adv. Exp. Med. Biol. (1999) 464: 127-147 and references cited therein.
• Antibody variants have also been produced in large amounts from transgenic plant seeds including antibody fragments, such as single chain antibodies (scFv's), including tobacco seeds and potato tubers. See, e.g., Conrad et al.(1998) Plant MoI. Biol. 38: 101-109 and reference cited therein. Ihus, antibodies of the present invention can also be produced using transgenic plants, according (o know methods.
• Antibody derivatives can be produced, for example, by adding exogenous sequences to modify immunogenicity or reduce, enhance or modify binding, affinity, on-rate, off-rate, avidity, specificity, half-life, or any other suitable characteristic. Generally part or all of the non-human or human CDR sequences are maintained while the non-human sequences of the variable and constant regions are replaced with human or other amino acids. In general, the CDR residues are directly and most substantially involved in influencing antigen binding.
• Ilumanization or engineering of antibodies of the present invention can be performed using any known method, such as but not limited to those described in U.S.
• Framework shuffling is another approach to humanization that allows for the identification of human framework combinations that will support the functional features of mouse or other animal CDRs, without the need for rational design or structural information.
• combinatorial Fab libraries are created by in-frame fusion of the CDRs of an animal MAb to pools of corresponding human frameworks that include all known heavy and light chain human germline genes. The Fab libraries may then be screened for antigen binding. The light and heavy chains of the parental Mab may be successively humanized in a further selection process. See Dall'Acqua et al. (2005) Humanized Antibodies and their Applications 36(l):43-60; the contents of which are incorporated by reference.
• Fully human antibody sequences are made in a transgenic mouse which has been engineered to express human heavy and light chain antibody genes. Multiple strains of such transgenic mice have been made which can produce different classes of antibodies. B cells from transgenic mice that are producing a desirable antibody can be fused to make hybridoma cell lines for continuous production of the desired antibody.
• Human monoclonal antibodies can also be produced by a hybridoma which includes a B cell obtained from a transgenic nonhuman animal, e.g., a transgenic mouse, having a genome including a human heavy chain transgene and a light chain transgene fused to an immortalized cell.
• the antibodies of this invention also can be modified to create chimeric antibodies. Chimeric antibodies are those in which the various domains of the antibodies' heavy and light chains are coded for by DNA from more than one species. See, e.g., U.S. 4,816,567.
• any recombinant antibody or the antibody fragment thereof according to the present invention may be used, so long as it can react specifically with at least two interferon alpha ("IFN ⁇ ") protein subtypes selected from the group consisting of protein subtypes A, 2, B2, C, F, G, H2, I, Jl , K, 4a, 4b and WA, but does not neutralize at least one bioactivity of TFN ⁇ protein subtype D.
• the antibody may also neutralize the IFN ⁇ , as measured in known bioassays, e.g., the bioactivity is activation of the MxA promoter or antiviral activity.
• One such antibody is an antibody that reacts specifically with one or more IFN ⁇ subtypes A, 2, B2, C, F, G, H2, T. JI , K, 4a. 4b and VVA, but not subtype D, and includes CDRs, derivatives or portions thereof selected from: [000126] V H 1 having the amino acid sequence of SKQ ID NO:4;
• V H 2 having the amino acid sequence of SEQ ID NO: 6;
• V H 3 having the amino acid sequence of SEQ ID NO: 8.
• V L 1 having the amino acid sequence of SEQ ID NO: 12;
• V 1 2 having the amino acid sequence of SEQ ID NO: 14; [000131
• V L 3 having the amino acid sequence of SEQ ID NO: 16; derivatives and combinations thereof.
• the antibodies may also include antibodies and/or antibody fragments in which one or more amino acids are deleted, added, substituted and/or inserted in these amino acid sequences and which specifically react with IFN ⁇ subtypes A, 2, B2, C, F, G, H2, I, Jl, K, 4a, 4b and WA are also included within the scope of the present invention.
• one or more amino acid deletions, substitutions, insertions or additions in the amino acid sequence refers to modifications and/or mutations of one or more amino acids that are deleted, substituted, inserted and/or added at one or more positions in the backbone of an immunoglobulin.
• the one or more deletions, substitutions, insertions and/or additions may be caused in the same amino acid sequence simultaneously.
• the amino acid residue substituted, inserted or added can be natural or non-natural.
• Examples of the natural amino acid residue include L-alanine, L-asparagine, E-aspartic acid, L-glutaninc, L-glutamic acid, glycine, L-histidine, L-isoleucine, L-leucine, L-lysine, L- methionine, L-phenylalanine, L-proline, L-serine, L-threonine, L-tryptophan, L-tyrosine, L- valine, L-cysteine, and the like.
• amino acid residues that may be substituted may be found within one or more of the following groups, for example: Group A: leucine, isoleucine, norleucine, valine, norvaline, alanine, 2-aminobutanoic acid, methionine, O-methyl serine, t-butyl glycine, t-butylalanine, cyclohexylalanine;
• Group B aspartic acid, glutamic acid, isoaspartic acid, isoglutamic acid, 2- aminoadipic acid, 2-aminosuberic acid: Group C: asparagine, glutamine;
• Group D lysine, arginine, ornithine, 2,4-diaminobutanoic acid, 2,3-diaminopropionic acid;
• Group E proline, 3-hydroxyproline, 4-hydroxyproiine
• Group F serine, threonine, homoserine
• Group G phenylalanine, tyrosine.
• linear antibodies further includes “linear antibodies”.
• linear antibodies include a pair of tandem Fd segments (V H -Cn 1-VH -C H I ) which form a pair of antigen binding regions.
• Linear antibodies can be bispecific or monospecific.
• the antibodies of this invention can be recovered and purified from recombinant cell cultures by known methods including, but not limited to, protein A purification, ammonium sulfate or ethanol precipitation, acid extraction, anion or cation exchange chromatography, phosphocellulose chromatography, hydrophobic interaction chromatography, affinity chromatography, hydroxylapatite chromatography and lectin chromatography.
• High performance liquid chromatography HPLC can also be used for purification.
• Antibodies of the present invention include naturally purified products, products of chemical synthetic procedures, and products produced by recombinant techniques from a eukaryotic host, including, for example, yeast, higher plant, insect and mammalian cells, or alternatively from a prokaryotic cells as described above.
• antibodies can be labeled with a detectable moiety such as a radioactive atom, a chromophore, a fluorophore, or the like.
• a detectable moiety such as a radioactive atom, a chromophore, a fluorophore, or the like.
• Such labeled antibodies can be used for diagnostic techniques, either in vivo, or in an isolated test sample.
• Antibodies can also be conjugated, for example, to a pharmaceutical agent, such as chemotherapeutic drug or a toxin. They can be linked to a cytokine, to a ligand, to another antibody.
• Suitable agents for coupling to antibodies to achieve an anti-tumor effect include cytokines, such as interleukin 2 (1L-2) and Tumor Necrosis Factor (TNF); photosensitizers, for use in photodynamic therapy, including aluminum (III) phthalocyanine tetrasulfonate, hematoporphyrin. and phthalocyanine; radionucleotides, such as iodine-131 ( Lil I), yttrium-90 ( 00 Y).
• cytokines such as interleukin 2 (1L-2) and Tumor Necrosis Factor (TNF)
• photosensitizers for use in photodynamic therapy, including aluminum (III) phthalocyanine tetrasulfonate, hematoporphyrin. and phthalocyanine
• radionucleotides such as iodine-131 ( Lil I), yttrium-90 ( 00 Y).
• antibiotics such as doxorubicin, adriamycin.
• daunorubicin methotrexate, daunomycin, neocarzinostatin, and carboplatin
• bacterial, plant, and other toxins such as diphtheria toxin, pseudomonas exotoxin A, staphylococcal enterotoxin A, abrin-A toxin, ricin A (deglycosylated ricin A and native ricin A), TGF-alpha toxin, cytotoxin from Chinese cobra (naja naja atra), and gelonin (a plant toxin)
• ribosome inactivating proteins from plants, bacteria and fungi such as restrictocin (a ribosome inactivating protein produced by Aspergillus r ⁇ striclus), saporin (a ribosome inactivating protein from Sapomiria officinalis), and RNase
• tyrosine kinase inhibitors ly207702 (a difluorinated purine nucleoside);
• An "activating group” is a chemical moiety or functional group that can, under appropriate conditions, react w r ith a second chemical group thereby forming a covalent bond between the modifying agent and the second chemical group.
• electrophilic groups such as tosylate, mesylate, halo (chloro, bromo, fluoro, iodo), N-hydroxysuccinimidyl esters (NHS), and the like.
• Activating groups that can react with thiols include, for example, maleimide, iodoacetyl, acrylolyl, pyridyl disulfides, 5-thiol-2-nitrobenzoic acid thiol (TNB-thiol), and the like.
• An aldehyde functional group can be coupled to amine- or hydrazide-containing molecules, and an azide group can react with a trivalent phosphorous group to form phosphoramidate or phosphorimide linkages.
• Suitable methods to introduce activating groups into molecules are known in the art (see for example, Hermanson (1996) BIOCONJUG ⁇ TE TECHNIQUES, Academic Press: San Diego, Calif.).
• An activating group can be bonded directly to the organic group (e.g., hydrophilic polymer, fatty acid, fatty acid ester), or through a linker moiety, for example a divalent C r Ci 2 group wherein one or more carbon atoms can be replaced by a heteroatom such as oxygen, nitrogen or sulfur.
• Suitable linker moieties include, for example, tetraethylene glycol.
• Modifying agents that include a linker moiety can be produced, for example, by reacting a mono-Boc-alkyldiamine (e.g., mono-Boc-ethylencdiamine, mono-Boc-diaminohexane) with a fatty acid in the presence of l-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) to form an amide bond between the free amine and the fatty acid carboxylate.
• a mono-Boc-alkyldiamine e.g., mono-Boc-ethylencdiamine, mono-Boc-diaminohexane
• EDC l-ethyl-3-(3-dimethylaminopropyl)carbodiimide
• the Boc protecting group can be removed from the product by treatment with trifluoroacetic acid (TFA) to expose a primary amine that can be coupled to another carboxylate as described, or can be reacted with maleic anhydride and the resulting product cyclized to produce an activated maleimido derivative of the fatty acid.
• TFA trifluoroacetic acid
• the modified antibodies of the invention can be produced by reacting a human antibody or antigen-binding fragment with a modifying agent.
• a modifying agent for example, the organic moieties can be bonded to the antibody in a non-site specific manner by employing an amine -reactive modifying agent, for example, an NHS ester of PEG.
• Modified human antibodies or antigen-binding fragments can also be prepared by reducing disulfide bonds
• Modified human antibodies and antigen-binding fragments including an organic moiety that is bonded to specific sites of an antibody of the present invention can be prepared using suitable methods, such as reverse proteolysis. See generally, Hermanson (1996) BlOCONJUGATF TECHNIQUES, Academic Press: San Diego, Calif. (1996).
• Polypeptides and proteins are necessary components of various methods of this invention.
• recombinant antibodies, variants, derivatives and fragments thereof can be obtained by chemical synthesis using a commercially available automated peptide synthesizer such as those manufactured by Perkin Elmer/Applied Biosystems, Inc., Model 430A or 43 IA, Foster City, CA, USA.
• the synthesized protein or polypeptide can be precipitated and further purified, for example by high performance liquid chromatography (HPLC).
• HPLC high performance liquid chromatography
• the proteins and polypeptides can be obtained by known recombinant methods as described herein using the host cell and vector systems described above. They can also be prepared by enzymatic digestion or cleavage of naturally occurring proteins.
• Proteins and peptides can be isolated or purified by standard methods including chromatography (e.g., ion exchange, affinity, and sizing column chromatography), centrifugation, differential solubility, or by any other standard technique for protein purification.
• affinity tags such as hexa-His (Invitrogen), Maltose binding domain (New England Biolabs). influenza coat sequence (Kolodziej, et al., (1991) Methods Enzymol. 194:508-509), and glulathione-S -transferase can be attached to the peptides of the invention to allow easy purification by passage over an appropriate affinity column.
• Isolated peptides can also be physically characterized using such techniques as proteolysis, nuclear magnetic resonance, and x-ray crystallography. [000143] It is well known that modifications can be made to any peptide to provide it with altered properties. Peptides for use in this invention can be modified to include unnatural amino acids. Thus, the peptides may include D-amino acids, a combination of D- and L-amino acids, and various "designer" amino acids (e.g., ⁇ -methyl amino acids, C- ⁇ - methyl amino acids, and N- ⁇ -methyl amino acids, etc.) to convey special properties to peptides.
• D-amino acids e.g., ⁇ -methyl amino acids, C- ⁇ - methyl amino acids, and N- ⁇ -methyl amino acids, etc.
• subunits of peptides that confer useful chemical and structural properties will be chosen.
• peptides including D-amino acids may be resistant to L-amino acid-specific proteases in vivo.
• Modified compounds with D-amino acids may be synthesized with the amino acids aligned in reverse order to produce the peptides of the invention as retro-inverso peptides.
• the present invention envisions preparing peptides that have better defined structural properties, and the use of peptidomimetics, and peptidomimetic bonds, such as ester bonds, to prepare peptides with novel properties.
• a peptide may be generated that incorporates a reduced peptide bond, i.e., R1 -CH 2 NH-R2, where Rl , and R2 are amino acid residues or sequences.
• ⁇ reduced peptide bond may be introduced as a dipeptide subunit.
• Such a molecule would be resistant to peptide bond hydrolysis, e.g., protease activity.
• Such molecules would provide peptides with unique function and activity, such as extended half- lives in vivo due to resistance to metabolic breakdown, or protease activity.
• constrained peptides show enhanced functional activity (Hruby (1982) Life Sciences 31 : 189-199 and IIruby et al. (1990) Biochem J. 268:249-262); the present invention provides a method to produce a constrained peptide that incorporates random sequences at all other positions.
• the ability of IFN ⁇ to activate the MxA promoter, and the ability of the anti-IFN ⁇ monoclonal antibodies of the invention to block this activation can be measured using reporter gene assays where the MxA promoter is fused to a reporter gene, such as chloramphenicol acetyltransferase (CAT) or lucifcrase (luc), preferably luciferase.
• CAT chloramphenicol acetyltransferase
• luc lucifcrase
• Assays for CAT and luciferase are known to those of skill in the art.
• the activity of the MxA promoter is measured in A549 cells stably transformed with an MxA promoter/reporter gene fusion construct.
• A549 cells are a lung carcinoma cell line available through the ATCC (product number CCl-185).
• the MxA (a.k.a. MxI) promoter can be human, mouse or rat.
• the sequence and structure of the human MxA promoter is disclosed in Genbank Accession number X55639, Chang et al. (1991) Arch Virol. 1 17:1 -15; and Ronni et al. (1998) J Interferon Cytokine Res. 18:773-781.
• Human MxA promoter/luciferase fusion constructs and luciferase assays are disclosed in U.S. patent application 20040209800 and Rosmorduc et al. (1999) J of Gen Virol 80: 1253-1262.
• MxA promoter/CAT fusion constructs and CAT assays arc disclosed in Fernandez et al. (2003) J Gen Virol 84:2073-2082 and Fray et al. (2001) J Immunol Methods 249:235-244.
• the mouse MxA (MxI) promoter is disclosed in Genbank accession number M21 104; Hug et al. (1988) MoI Cell Biol 8:3065- 3079; and Lleonart et al. (1990) Biotechnology 8: 1263-1267.
• a mouse MxA promoter/luciferase fusion construct and a luciferase assay are disclosed in Canosi et al. (1996) J Immunol Methods 199:69-67.
• IFN- ⁇ A (3.8 x 10 8 U/mg); IFN- ⁇ 2 (2.77 x 10 s U/mg); IFN- ⁇ B2 (4.63 x 10 8 U/mg); IFN- ⁇ C (2.31 x 10 8 U/mg); IFN- ⁇ D (7.5 x 10 7 U/mg); IFN- ⁇ F (3.6 x 10 s U/mg); IFN- ⁇ G (2.33 x 10 8 U/mg); IFN- ⁇ H2 (1.05 x 10 8 U/mg); IFN- ⁇ I (1 .4 x Kf U/mg); IFN- ⁇ Jl (2.6 x 10 8 U/mg); IFN- ⁇ K (1.48 x 10 s U/mg); IFN- ⁇ 1 (1.4 x 10 s
• PBMC-flu supernatant which contains a complex mixture of human IFN ⁇ subtypes
• PBMC-flu was prepared in-house by infection of human PBMCs from buffy coats with Influenza A/PR/8/34 (HlNl) (Charles River Laboratories, Lot #4XPR011022) at a viral titer of 1 HAU/pDC.
• PBMCs peripheral blood monocytic cells
• PBMCs peripheral blood monocytic cells
• FACS staining/analysis was performed to confirm the presence of plasmacytoid DCs and determine their percentage within the PBMCs and stained with fluorescence-conjugated antibodies specific for Lin, CD3, CD14, CD16, CD19, CD56, CD123, IILA-DR, and CDl Ic (BD Pharmingen).
• pDCs were characterized as CD14 negative, CDl Ic negative and CD123 positive.
• the flu virus stock (Specific Pathogen-Free Avian Supply; Influenza A/PR/8/34 (Hl Nl ) (Cat.
• PBMCs prepared from the buffy coat were centrifuged at 900 rpm for 10 min and resuspended at 5,000 cells/ ⁇ L (this will provide for 1 x 10 6 cells per well in a volume of 200 ⁇ L) in RPMI + 10% FCS + L-glutamine).
• the cells plus flu vims were plated in 96-well U-bottom plates, and incubated at 37°C + 5% CO 2 for 24 h. Following incubation for 24 h, the cells formed a pellet at the bottom of the wells and have formed clusters. The supernatant was harvested from the wells by careful pipetting, avoiding cell pellets at the bottom of the wells.
• PBMC-flu supernatants containing a complex mixture of IFN ⁇ , were pooled, mixed, and stored at -8O 0 C in 0.5 ml aliquots until use.
• CPE Cvtopathic Effect
• CPE Materials Dulbccco's Modified Eagle ' s Medium (DMEM) "complete”: DMEM with phenol red h 10% FCS + 2 mM L-glutamine + penicillin + streptomycin + ⁇ -2-mercaptoethanol ( ⁇ -me), 96-well flat- bottom tissue culture plates, A549 cells (A TCC CCL-185): these cells are cultured in Ham's F12K medium + 10% FCS + 2 mM L-glutamine • + • penicillin + streptomycin + 500-800 ⁇ g G418, Ix PBS, Ix trypsin, "Intron A” (IFN ⁇ -2b, Schering-Plough) controls, test samples (SLE serum, recombinant IFN ⁇ subtypes) with/without hybridoma supernatant or purchased polyclonal/monoclonal antibody preparations, EMC virus stocks (prepared from the murine encephalo
• Crystal violet stock solution 1.25 mg NaCl + 3.75 mg crystal violet + 775 mL formaldehyde/ethanol solution (which is prepared with 75 mL formaldehyde, 750 mL 95% ethanol, and 1500 mL distilled water) were stirred for 20 min, then filtered through a 0.45 micron filter and stored for not longer than 3 months.
• a working solution of Crystal violet was prepared by diluting the stock solution 1: 10 with the formaldehyde/ethanol solution. The crystal violet solutions were stored at room temperature.
• CPE Assay
• Figure 1 CPE assays were performed in triplicate wells in 96-well flat-bottom plates. For each assay type, it was useful to incorporate positive control wells containing intron A (Schering-Plough) samples of varying concentrations and negative control wells containing only cells + media.
• Adherent A549 cells were harvested from flasks by removing the culture media, washing once with PBS, and trypsinization. Trypsinization was stopped by adding DMEM "complete" to the flask. The tyrpsinized cells were collected from the flask, centrifuged, resuspended and counted. Their concentration was adjusted to 600,000 cclls/mE in complete DMEM.
• the volume of the wells for the assay was 150 ⁇ L (prior to virus addition) and 200 ⁇ L (following virus addition). 50 ⁇ L of the volume was cells, of which 15,000 cells (50 ⁇ L of the 300,000/mL cell suspension) was added per well.
• DMEM Dulbecco's Modified Eagle's Medium
• DMEM Dulbecco's Modified Eagle's Medium
• DMEM Dulbecco's Modified Eagle's Medium
• RG Methods A l ⁇ ciferase-based reporter gene assay was utilized to evaluate the ability of the anti-IFN ⁇ MAbs to neutralize the bioactivity of recombinant IFN ⁇ subtypes, leukocyte TFN and PBMC-flu.
• ⁇ schematic diagram of the RG assay is shown in Figure 1.
• the 93D7 cell line which was derived by stable transfection of the A549 cell line (CLL-185, ATCC) with an TFN-inducible construct (MxA promoter/luciferase fusion) was kindly provided by Dr. Guenther Adolf (Boehringer-Ingelheim GmbH, Austria).
• the MxA promoter/1 uc fusion vector includes a 1.6 Kb BamIII fragment containing the murine MxA promoter and IFN response elements excised from pSP64-Mxp(PstI-Pvu ⁇ I)-r ⁇ glo (Lleonart et al. (1990) Biotechnology 8: 1263-1267) and inserted upstream of a luciferase coding sequence.
• adherent 93D7 cells were harvested from flasks by removing the culture media, washing once with PBS, and trypsinization. Trypsinization was stopped by adding DMEM "complete" to the flask. The cells were collected from the flask, centrifuged, resuspended and counted. Their concentration was adjusted to 600,000/mL in complete DMEM.
• the volume/well was then adjusted to 100 ⁇ L by adding DMEM without FCS (note: any time serum samples of any type are added to wells using this assay, DMEM without FCS will be used) and then 50 ⁇ L of cells was added and incubated for 5 hours at 37 0 C + 5% CO 2 .
• the plates were incubated for 1 .5 hours at 37 0 C + 5% CO 2 . After this lime, 50 ⁇ L of cells was added and incubation continued for 5 hours at 37 0 C + 5% CO 2 .
• BriteliteTM kit reagents/developing reagents were set at room temperature 40 min prior to assay development. At 30 min pre-development, the assay plates were placed at room temperature. At 10 min pre- development. the lyophilized substrate was reconstituted with buffer (10 mL per vial).
• Example 1 Immunization and Selection of Monoclonal Antibody Cell Lines.
• a flow chart of the IFN ⁇ MAb development scheme is shown in Figure 2.
• Groups of five 6-8 week old Balb/c female mice (Harlan) were immunized with 5-10 ⁇ g each natural leukocyte IFN ⁇ (1-2396, Lot #1 1 1K1603, Sigma) and/or a cocktail of recombinant proteins (5-10 ⁇ g each of the three recombinant 1I 7 N ⁇ subtypes A, B2, and F (obtained from PBL Biomedical Laboratories "PBL”)) in MPL ® + TDM emulsion (Sigma #M6536) at two to three week intervals according to the schedules indicated in Table 1, below.
• PBL PBL Biomedical Laboratories
• the MPL ® + TDM emulsion is a Ribi Adjuvant system consisting of monophosphoryl -lipid A (MPL: detoxified endotoxin from S. Minnesota) and trehalose dicorynomycolate (TDM) in a 2% oil (squalene)-Tween 80- water emulsion.
• Antigen was administered via intraperitoneal (i.p.) or subcutaneous (s.c.) routes.
• Pre-fusion screening of serum collected from the mice was done at three titers (1 :200, 1 :2000, and 1 :20,000) using the reporter gene (RG) assay, based upon a MxA-luciferase fusion protein via activation of the Type I IFN receptor, to detect blockade of IFN ⁇ bioactivity.
• Serum was collected from the mice via retro-orbital bleed seven days following the third boost and screen for neutralization of PBMC-flu bioactivity using the reporter gene (RG) assay described above. Mice exhibiting titers of at least 1 :2000 for > 50% neutralization were rested for four weeks and then given a final boost (either i.v.
• mice immunized 17 were able to neutralize the PBMC-flu supernatant by at least 50% at titers of 1:200, among these, 14 could neutralize at > 50% at 1 :2000 dilutions, and 3 continued to neutralize at titers up to 1:20000.
• the fusions were performed pooling the cells from 2-3 mice within a protocol. Two mice (initially immunized with IFN ⁇ F in Protocol 6 were pooled to make fusion 8). In Protocol 5, two mice were pooled to make fusion 7, and three were pooled to make fusion 9.
• mice were identified as candidates for fusion based upon the ability of their serum to neutralize the complex mixture of IFN ⁇ subtypes present in PBMC-flu.
• a series of 8 fusions were performed with splenocytes harvested from mice with acceptable serum titers.
• Splenocytes were fused with the Sp2/0-Agl4 murine myeloma cell line (ATCC Number CRL-1581, which was selected since it is unable to express endogenously-derived Ig chains), plated in 96-well flat-bottom tissue culture plates, and incubated for 12-15 days prior to screening of supernatants in order to detect a polyclonal antibody response via the RG assay protocol described above.
• Hybridoma cell lines were subcloned by limiting dilution.
• Hybridomas producing anti-IFN- ⁇ MAbs were adapted to growth in Gibco PFHM-II (Invitrogc ⁇ ) and cultured in Integra CRIJ vine flasks (Becton Dickinson). Supernatants were collected from the cell compartments every 5-7 days and frozen at -80 0 C. The MAbs were then purified from 50 ml batches of supernatant via FPLC over protein A columns followed by dialysis into PBS. The purified MAbs were aliquotted and stored at -80 0 C.
• ACO-I, 2, 3, 4, 5, and 8 were isotyped by ELISA as IgG2a (ACO-I), IgG2b (ACO-2), and IgGl (ACO-3, 4, 5, 6 and 8). All of these candidates derived from fusions of splenocytes from mice initially immunized with leukocyte IFN or a mixture of IFN - ⁇ A, B2, and F recombinant proteins followed by a pre-fusion boost with leukocyte IFN: fusions performed from mice administered only the recombinant IFN- ⁇ subtypes failed to yield any candidates able to neutralize PBMC-flu.
• the anti- lFN- ⁇ MAbs shown to strongly bind and neutralize at least one IFN- ⁇ subtype were selected to examine their abilities to neutralize naturally-derived IFN preparations, which are known to contain a broad variety of IFN- ⁇ subtypes.
• ACO-I through 5 were titrated in the RG bioassay against both commercially-available leukocyte IFN and PBMC-flu supernatant prepared as describe above. ACO-I , 2.
• the neutralizing units (U) provided by the manufacturer has been assigned via an assay measuring the ability of the given subtypes to neutralize 50% (identified as 1 U/ml) of the cytopathic effect produced by vesicular stomatitis virus on bovine MDBK cells.
• IFN- ⁇ potencies in bioassays are influenced by numerous variables (including assay type, individually prepared batches, and minor technique variations from one laboratory to another), as well as the fact that internationally-recognized standards for each subtype are unavailable, single lot numbers were consistently employed in these studies.
• the manufacturer-defined U of each recombinant that would yield maximal response in the RG bioassay was determined.
• Representative RG bioassay data for the titration of ACO-I against the RG niax values of the fifteen IFN - ⁇ subtypes is shown in Figure 4a. As described in the legend, numerical values were assigned to each ACO-I titration against the designated subtypes based upon the FX] 50 values determined for each.
• ACO-I was unable to neutralize either IFN- ⁇ D or IFN- ⁇ 1 , whereas the other thirteen subtypes could be neutralized at various antibody concentrations.
• the EC 50 results in ng/ml for all ⁇ CO-1, 2, 3, 4, 5 and 8 IFN- ⁇ -neutralizing MAbs are provided in Table 4.
• the percentage neutralization is shown in Table 5. Accordingly, ACO-I and 2 appear similar in their capacities to neutralize twelve subtypes (IFN- ⁇ A, 2, B2, C, F, G, ⁇ I2, 1, Jl, 4a. 4b, and WA) at concentrations of less than 300 ng/ml of antibody; ⁇ CO-1 also neutralized IFN- ⁇ K to this extent, though ACO-2 did not.
• ACO-3 and 4 neutralized nine (IFN- ⁇ A, 2, B2, C, I, Jl, K, 4a, and WA) and six (IFN- ⁇ A, 2, B2, C, 1, J l. and 4a) subtypes with less than 300 ng/ml, respectively.
• ACO-8 neutralized four subtypes (IFN- ⁇ 2, 1 , 4a, and 4b) given the antibody concentration constraint, while ACO-5 strongly neutralized only three (IFN- ⁇ A, 2, and WA). None of the MAbs were able to neutralize IFN-P (Figure 4b).
• the CPE assay was set up similarly to the RG assay with untransfected A549 cells (ATCC Number CCL-185. a human lung carcinoma cell line): assays are performed in standard 96-well flat-bottom tissue culture plates. Following pre-incubation (1 hour at 37°C) of antibodies with the IFN ⁇ subtypes and addition of cells 5 hours later, mouse encephalomyocarditis virus (EMCV) was added and the cells were incubated for 48 hours prior to staining with crystal violet for assessment of live cells remaining.
• EMCV mouse encephalomyocarditis virus
• the quantities of each ⁇ FN ⁇ subtype used were determined via prior titrations of the recombinant IFN ⁇ proteins to yield either maximal MxA-luciferase induction (RG) or protection from cell death (CPE) in the assay.
• the data shown in Table 6 represents the percentage of bioactivity blockade demonstrated by each ACO monoclonal antibody against the respective IFN ⁇ subtypes (corresponding genes encoding the subtypes are indicated in parentheses) in the CPE assay.
• ACC-I and ACO-2 are capable of blocking the largest number of IFN ⁇ subtypes at levels of > 90% under the assay conditions, whereas ACO-6 is the most restricted. In most cases, the outcomes of the RG and CPE assays correlate with one another.
• Example 4 Multiplex analysis of ACO- 1, ⁇ CO-2, ⁇ CO-3. ACQ-4, ACO-5. and ACO-6. Multiplex analysis was conducted to assess whether spatially distinct binding domains were involved.
• the ACO antibodies were analyzed combinatorially for their abilities to simultaneously bind IFNa-A via multiplex analysis on a Luminex 1M 100 system. Beads coupled with unlabeled ACO antibodies (Capture) were incubated with recombinant IFNa-A at the concentration indicated and then exposed to PE-labeled ACO antibodies (Reporter). This examination revealed that ACO-5 can multiplex with any of ACOs -1, -2, -3, and -4 (see light shading in Figure 5).
• ACO-4 is employed as a capture antibody and ACO-3 as a reporter antibody. Accordingly, ACO-5 binds a spatially distinct domain of TFNa-A than that bound by ACO-I, 2, -3 and -4. Similarly, ACO-3 and ACO-4 bind spatially distinct domains of IFNa-A. Results with ACO- 6 were negative in all cases. [000181 ] Example 5. Affinity determinations for ACQ-I, ACO-2, ACO-3, ACO-4.
• the K D values for the five anti-IFN- ⁇ MAbs covered a range inversely proportional to the breadth of IFN- ⁇ subtypes neutralized by each MAh as well as their potency in blocking leukocyte IFN and PBMC-flu bioactivily.
• ACO-I exhibited the lowest affinity (5.61 x 10 9 M), while ACO-5 exhibited a 14-fold higher affinity (4.00 x 10 "10 M).
• ⁇ CO-6 does not bind to IFN ⁇ - ⁇ and therefore no rates were obtainable.
• Table 7 Biacore Kinetic Analysis of ACO-I through ACO-6 with ⁇ FN- ⁇ A
• the assays were developed by incubation w r ith 50 ⁇ l/well of an HRP conjugated goat anti-mouse-IgG (Jackson ImmunoResearch) at room temperature for 30 minutes followed by incubation with 100 ⁇ l/well of TMB substrate solution (Zymed) for 15 minutes. The reaction was stopped with 100 ⁇ l/well of 1 N HCl and read at OD 450 on an ELISA plate reader. Binding percentages were calculated by normalizing background signal values with maximal signal values across the assay (observed for IFN ⁇ -4a). The results are shown in Table 8 and Figure 6.
• Both ACO- 1 and 2 bound the identical twelve IFN- ⁇ subtypes that they effectively neutralized in the RG bioassay at signals at least 2-fold greater than isotype-matched controls; binding of IFN- ⁇ subtypes B2, K, 4a, and 4b demonstrated signals more than 20-fold over controls. Differences between binding and neutralization capacities were observed, however, among ACO-3, 4, 5, and 8.
• the ELISA signals for subtypes B2, K, and 4a were the highest, despite the fact that the EC 50 value for neutralization of IFN- ⁇ K was greater than 200-fold higher that the EC 50 for IFN- ⁇ B2 and 4-fold higher for IFN- ⁇ 4a; significant binding of subtype J l, which was neutralized in the bioassay was not detectable.
• the binding and neutralization profiles for ACO-4 and 5 presented inverse relationships with one another. While ACO-4 and 5 strongly bound one subtype each (TFN- ⁇ 4a and 2, respectively), ACO-4 was able to bind more subtypes than it neutralized, and ACO-5 was able to neutralize (albeit at high EC 50 values) more subtypes than it bound.
• ⁇ CO-8 failed to strongly bind any of the IFN- ⁇ subtypes tested; it exhibited binding of less that 20-fold to IFN- ⁇ D, 1, and 4a.
• ACO-6 failed to bind to any of the subtypes. TABLE 8. Binding of recombinant IFN- ⁇ subtypes by ACO-I, 2, 3, 4, 5, and 8.
• Example 7 Blockade of SLE patient serum bioactivity by ACO-I , 2 and 3 monoclonal antibodies.
• An antiviral assay was used to evaluate the ability of the anti-IFN- ⁇ MAbs to neutralize the protective activity of serum from SLE patients with active disease against the death of A540 cells (CCL-185, ATCC) upon infection with encephalomyocarditis virus (RMCV).
• the antibodies exhibiting the broadest IFN- ⁇ subtype, leukocyte IFN, and PBMC-flu neutralization profiles (ACO-I, 2 and 3) were tested.
• the SLE serum was obtained from four active SLE patients (identified as SLE-43, 133, 140 and BC) selected upon the basis of IFN and granulopoiesis gene expression signatures characterized from their blood mononuclear cells. SLE sera were screen for protection against viral infection in the CPE bioassay prior to MAb neutralization testing. The RG assay was not employed in these analyses due to inhibition by serum factors of cell binding to the ViewPlates 1 M during the comparatively shorter incubation period (5 hours) of the RG bioassay versus the CPE assay (48 hours). Vero cells (CCL-81, ATCC) were infected with EMCV (VR-129B, ATCC) to prepare working viral stocks from supernatants.
• Assays were performed in triplicate in tissue culture-treated, flat-bottom 96-well plated incubated at 37 0 C + CO 2 with A549 cells (15,000 cells/well in 50 ⁇ l each) overnight. Anti-IFN- ⁇ MAbs and serum from SLE patients were then added to the plated (100 ⁇ l/well) and prcincubated for 4 hours prior to addition of EMCV diluted to the minimal concentration in 50 ⁇ l able to kill 100% of unprotected cells in 48 hours. Incubation was continued for 48 hours, followed by staining with crystal violet and reading at OD 570 in an ELISA plate reader.
• Controls were serum alone, media only (-), and a pan-neutralizing polyclonal antibody (pAb, rabbit anti-human IFN- ⁇ , PBL).
• pAb pan-neutralizing polyclonal antibody
• Figure 7a-d represent the mean of triplicates.
• ACO-I and 2 were capable of neutralizing all four sera to some degree.
• ACO-3 was unable to block SLE-43, 140 or BC,
• the ability for relevant isotype control antibodies to block serum in some instances (lgG2b for SLE-43, all three isotypes for SLE-BC) likely resulted from natural variations in other scrum constituents from one patient to another that were cytotoxic to the cells employed in the assay.
• Example 8 Cross Reactivity of ACO-I and ACO-2 with primate IFN- ⁇ .
• the ability of two candidates, murine anti-human IFN- ⁇ Abs ACO-I and ACO-2, to neutralize primate IFN- ⁇ were tested.
• the ability of the antibodies to block induction of an MxA-luciferase reporter gene in A549 cells when stimulated with purified macaque IFN- ⁇ 4b (156 pg/well) was determined.
• the antibodies ACO- 1 and ACO-2 potently block reporter gene induction (A and B, respectively) while ACO-3 is unable to block even at high concentrations (C).
• Homology between human and Macaque IFN- ⁇ is highly conserved.
• commercially available anti-human IFN- ⁇ antibodies have been shown to cross-react with Rhesus and cynomologous homologs. These data suggest that primates provide a suitable safety screening model.
• Example 9 Sequence of ACO-I heavy and light chains.
• RT/PCR was performed using degenerate primer pools to amplify mRN ⁇ from the hybridoma expressing ACO-I .
• Heavy chain variable region mRNA was amplified using a set of six degenerate primer pools (HA to HG) and light chain variable region mRN ⁇ was amplified using a set of eight degenerate primer pools (LA to LI).
• Amplification products were obtained with primer pools: HA. HB, HE, HF, LB, LC and LG.
• No PCR product was amplified with pool LT, therefore the light chain is from the kappa cluster. Each product was cloned and several clones from each scquenced.
• HA and HF codes for a full length mouse Vi, region as shown in Figure 9.
• the full length heavy chain DNA sequence is SEQ ID NO: 1, and the full length amino acid sequence is SEQ ID NO:2.
• V H 1 YTFTNYWMH; SEQ ID NO:4
• V ⁇ 2 EINPSHGRI f YN ENFKS; SEQ ID NO:6
• V U 3 GGLGPAWFA Y: SEQ ID NO:8
• V L 1 AGTGCCGGCTCAAGTGTAGATTCCAGCTATTTGTAC; SEQ ID NO: 1 1
• V L 2 AGC ACATCCAACCTGGCTTCT; SEQ ID NO: 13
• Humanized antibody is made by grafting the murine complementarity- determining regions into a human antibody framework (CDR-grafting) using methods known in the art (See Jones, et al., (1986) Nature, 321 :522-525; Reichmann et al., (1988) Nature, 332:323-329; Presta (1992) Curr. Op. Struct. Biol., 2:593-596; and Clark (2000) Immunol. Today 21 : 397-402).
• the humanized antibody may be capable of the same binding and functional parameters as the murine monoclonal antibodies described above.
• Example 1 Treatment of SLE using humanized monoclonal antibody.
• Microarray analysis will be used to monitor the IFN ⁇ signature according to methods known in the art and described in Bennett, et al. (2003) supra and Baechler, et al. (2003) supra. This new tool will serve to stratify (i.e. positive IFNa signature inclusion criteria), as well as monitor patients. Use of this analysis also is useful to determining which patients are suitably treated by the compositions and methods of this invention. In one aspect, administration of an antibody of this invention will extinguish this signature.
• one of skill in the art can determine when the object of a method of this invention is met, and an effective amount of antibody has been delivered, when is defined as the amount required the EFNa signature is suppressed by > 50% for an effective amount of time, e.g. about 4 weeks.
• An effective amount will be infused, e.g., from about lmg/kg, the second 2.5mg/kg, the third 5mg/kg antibody, and a fourth, if necessary, will be at 10mg/kg.
• the "calculated optimal dose" for each patient is defined as the amount that can be safely administered and gives at least 50% suppression of the IFN ⁇ signature for about four weeks.
• Acceptable methods include, but are not limited to microarray analysis of PBMCs (efficacy is established upon extinction of the interferon signature), flow cytometry of PBMCs (efficacy is established by increased T/B lymphocyte counts), decreased plasmacytosis and decreased presence of immature neutrophils or cytokine multiplex analysis in serum by use of luminex analysis.
• ACO-I, ACO-3 and ACO-6 hybridoma cell lines were deposited with the American Type Culture Collection, 10801 University Boulevard., Manassas, VA 20110-2209, USA (ATCC), and were accorded the deposit numbers listed in Table 10 below.
• a deposit of ⁇ CO-2 (labeled ACO2.2.1R) was deposited with the ATCC on August 9, 2006. Table 10
• ACO-2 (deposit ACO2.2. IR) PT ⁇ -7778 08/03/2006 ⁇ CO-3 PTA-6559 02/08/2005
• compositions and/or methods disclosed and claimed herein can be made and executed without undue experimentation in light of the present disclosure. While the compositions and methods of this invention have been described in terms of preferred embodiments, it will be apparent to those of skill in the art that variations may be applied to the compositions and/or methods and in the steps or in the sequence of steps of the method described herein without departing from the concept, spirit and scope of the invention. More specifically, it will be apparent that certain agents which are both chemically and physiologically related may be substituted for the agents described herein while the same or similar results would be achieved. All such similar substitutes and modifications apparent to those skilled in the art are deemed to be within the spirit, scope and concept of the invention as defined by the appended claims.

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