WO2008012952A1

WO2008012952A1 - Composition for stimulation of metabolism

Info

Publication number: WO2008012952A1
Application number: PCT/JP2007/000804
Authority: WO
Inventors: Yoshitake Baba; Akihiro Abe; Ayumu Nozawa; Yuko Sagesaka; Takami Kakuda; Noritaka Okamura; Shojiro Tsukamoto; Taizo Kubo
Original assignee: Ito En, Ltd.
Priority date: 2006-07-28
Filing date: 2007-07-27
Publication date: 2008-01-31

Abstract

Disclosed is a composition for stimulating metabolism, comprising teanin as an active ingredient. Also disclosed is a beverage/food comprising the composition. Teanin has metabolism-stimulating effects including the enhancement of the lipid utilization, the stimulation of the secretion of a growth hormone, the increase in blood glucose level, the burning of fats, the alleviation of fatigue during aerobic exercises, the increase in anaerobic threshold and the reduction in blood lactate accumulation.

Description

Specification

Metabolic promoting composition

Technical field

[0001] The present invention relates to a metabolism-promoting composition containing theanine as an active ingredient, and more particularly to a metabolism-promoting composition characterized in that a metabolism-promoting action is produced by the fat utilization promoting action of theanine. In addition, the present invention has the effect of promoting metabolism, improving endurance, promoting growth hormone secretion, increasing blood glucose level, fat burning, reducing fatigue during aerobic exercise, increasing anaerobic metabolic threshold, reducing blood lactate accumulation, anti-fatigue It is related with the metabolism promotion composition characterized by being, and the food / beverage which mix | blended this composition. Furthermore, the present invention relates to a method for promoting metabolism characterized by orally ingesting such a composition or food or drink.

[0002] Metabolism generally refers to a chemical reaction in a living body, and obtains energy by synthesizing a substance taken from outside the body into another substance. As metabolism, energy metabolism (primary metabolism), secondary metabolism, drug metabolism, etc. are known, and in order to maintain homeostasis, the mechanism (system) involved in metabolism must be kept in harmony. Metabolism is known to involve processes such as anabolism and catabolism, and is involved in various actions in vivo.

[0003] Energy metabolism is also called central metabolism or primary metabolism. Energy metabolism involves both catabolic and catabolic reactions related to sugars, amino acids and fatty acids. On the other hand, secondary metabolism is related to the production of substances that are not involved in the central metabolic system. Drug metabolism (foreign substance metabolism) involves detoxification and detoxification of in vitro substances such as poisons and drugs. In the present invention, metabolism refers to energy metabolism or secondary metabolism, and does not include drug metabolism (foreign substance metabolism).

[0004] Many substances having an action of promoting energy metabolism are already known. Although it varies somewhat depending on the substance, the promotion of metabolism in the living body involves the use of lipids in the body, reducing fatigue, strengthening nutrition, improving endurance, and accumulating lactic acid in blood. Effects such as reduction and fat burning may occur. Along with this, hormone secretion may be promoted, resulting in effects such as an increase in blood glucose level, fatigue reduction, nutrition tonicity, endurance improvement, blood lactate accumulation reduction, and fat burning. Furthermore, as energy metabolism is promoted, an anaerobic metabolic threshold may be increased and blood lactate accumulation may be reduced.

[0005] Especially when it comes to reducing fatigue, there are physical fatigue and mental fatigue. Generally, physical fatigue means fatigue and accumulation of physical activity, energy consumption, mental fatigue. Is said to mean fatigue due to brain activity and nerve activity.

[0006] Since physical fatigue and mental fatigue often coexist, it is often impossible to determine which type of fatigue is based on symptoms, but the mechanisms that cause fatigue, symptoms, mechanisms for reducing fatigue, etc. Each is different. For example, physical fatigue is sleepy, dull, and so on, but the head becomes a bit of a puff, whereas in mental fatigue, the nerves are rather excited, making it easy to get angry, sleep It will be in a state of poor concentration.

[0007] Such fatigue functions as an alarm for protecting the body and mind from excessive abuse, and is an in-vivo mechanism indispensable for maintaining a living body. In particular, body fatigue functions as a vital defense system that prevents chronic fatigue and overwork death. In other words, when the brain senses that the body is impossible, the information is transmitted to the brain stem class body, from the brain stem class body to the hypothalamus, which is the center of the autonomic nervous system, as well as instincts such as appetite and sexual desire. It is transmitted to the limbic system that controls emotions, and further transmitted to the cerebral cortex that controls movement and the five senses, where information is analyzed and judged, and the command is transferred from the central nervous system to the autonomic nervous system, immune system, and endocrine system. It is transmitted and brings about physical changes. For example, when the muscles are overworked with sports, the information is transmitted from the muscles to the brain, and the information is transmitted from the muscles to the brain, causing a feeling of fatigue, and causing muscle pain in the limbs.

[0008] If physical fatigue is moderate, it can be recovered by resting or sleeping, but if excessive physical fatigue or chronic fatigue occurs, recovery is difficult only by resting or sleeping. It is also necessary to actively reduce physical fatigue due to external factors. Also, by removing or reducing unpleasant sensations such as fatigue caused by physical fatigue, sleep becomes more effective when rested. Furthermore, if physical fatigue can be recovered in a shorter time, the next activity can be performed efficiently without feeling tired. Thus, although body fatigue is indispensable as a biological defense system, there is also a need to recover fatigue efficiently and easily.

[0009] As described above, many substances and compositions that promote such energy metabolism have been known so far. Plant-derived energy metabolism promoting substances ■ Even if limited to compositions, for example, isoflavones derived from soybeans (Patent Document 1), kabushinoids derived from pepper (Patent Document 2), guava leaf extract (Patent Document 3), grapes Origin of resveratrol (Patent Document 4) and the like are already known.

[0010] In particular, with regard to fatigue reduction, as drugs and foods and drinks for reducing such physical fatigue, a nutrient that serves as an energy source (Patent Document 5) and a feeling of fatigue due to the excitement effect are provided. What was masked (Patent Document 6) was known.

[001 1] However, in either case, physical fatigue was not directly reduced, and in the former case, there was a risk of excessive calorie intake. In the latter case, there was a problem that when the masking effect was cut off, the tiredness was further increased. In addition, it has recently been pointed out that continuing to suppress fatigue with the masking effect over a long period of time can lead to serious problems such as death from overwork.

(Non-Patent Document 1)

Incidentally, as described later, the present invention uses theanine as an active ingredient. Conventionally, theanine has a relaxing effect (Patent Document 7) and a mental fatigue recovery effect (Patent Document 8). It is also known that theanine recovers fatigue associated with decreased blood flow (Patent Document 9). However, it has not been reported so far that theanine produces an energy metabolism promoting effect by promoting energy metabolism, particularly lipid utilization promoting action.

Patent Document 1: Japanese Patent Application Laid-Open No. 2 0202 _ 2 0 3 0 4 Patent Document 2: Japanese Patent Laid-Open No. 2 0 0 4 _ 1 4 9 4 9 4

Patent Document 3: Japanese Patent Laid-Open No. 2 0 0 5 _ 1 3 0 0 6

Patent Document 4: Japanese Laid-Open Patent Publication No. 2 0 0 7 _ 1 4 5 8 0 9

Patent Document 5: Japanese Patent Laid-Open No. 2 0 0 5 _ 0 9 7 1 6 1

Patent Document 6: Japanese Patent Laid-Open No. 09-9-0 5 9 1 6 1

Patent Document 7: Japanese Patent Laid-Open No. 2 0 0 2 _ 3 7 0 9 7 9

Patent Document 8: Japanese Patent Laid-Open No. 2 0 0 5 _ 1 8 7 3 4 4

Patent Document 9: Japanese Patent Laid-Open No. 2 0 0 1 _ 3 1 6 2 5 6

Non-patent document 1: Separate volume ■ History of medicine, science of fatigue, 2 0 0 1

Disclosure of the invention

Problems to be solved by the invention

[0014] An object of the present invention is to provide a highly safe energy metabolism promoting composition, in particular a metabolism promoting composition in which energy metabolism is promoted by a lipid utilization promoting action, a food or drink containing the metabolism promoting composition, An object of the present invention is to provide a method for promoting metabolism by orally ingesting such a metabolism promoting composition and food and drink.

Means for solving the problem

[0015] The present inventors have found that theanine has an energy metabolism promoting action, particularly an energy metabolism promoting action by a lipid utilization promoting action, and has completed the present invention. Theanine also helps to improve endurance, promote growth hormone secretion, increase blood sugar levels, burn fat, reduce fatigue during aerobic exercise, increase anaerobic metabolic threshold, reduce blood lactate accumulation, and anti-fatigue. As a result, the present invention has been completed.

[001 6] That is, the present invention provides:

(1) A metabolism promoting composition comprising theanine as an active ingredient,

(2) The metabolism promoting composition according to the above (1), characterized in that metabolism promoting action is caused by the lipid utilization promoting action of theanine,

(3) Metabolism-promoting action improves endurance, promotes growth hormone secretion, increases blood glucose levels, fat burning, reduces fatigue during aerobic exercise, increases anaerobic metabolic threshold, blood lactate accumulation The metabolism promoting composition according to the above (1) or (2), characterized in that it is selected from the group consisting of reducing fatigue and anti-fatigue,

(4) The metabolism promoting composition according to any one of (1) to (3) above, which contains 0.5 to 5 mg / kg theanine per 1 kg body weight,

(5) The metabolism promoting composition according to any one of the above (1) to (4), comprising 6 to 600 Omg of theanine,

(6) A food or drink containing the metabolism promoting composition according to any one of (1) to (5) above,

(7) A method for promoting metabolism, comprising orally ingesting the composition for promoting metabolism according to any one of (1) to (6) above,

(8) Metabolism promoting action from lipid utilization promotion, growth hormone secretion promotion, blood sugar level increase, fat burning, fatigue reduction during aerobic exercise, anaerobic metabolic threshold increase, blood lactate accumulation reduction, anti-fatigue The method of promoting metabolism according to (7) above, wherein the method is one or more selected from the group consisting of:

(9) The method for promoting metabolism according to (7) or (8) above, wherein the dose of theanine is 0.5 to 5 mg / kg body weight per kilogram per administration.

The invention's effect

[0017] According to the present invention, a highly safe energy metabolism promoting composition, particularly a metabolism promoting composition in which energy metabolism is promoted by a lipid utilization promoting action, a food and drink containing the metabolism promoting composition, It is possible to provide a method for promoting metabolism by orally ingesting such a metabolism promoting composition or food and drink.

BEST MODE FOR CARRYING OUT THE INVENTION

[0018] In this section, as an embodiment of the present invention, a metabolism promoting composition and a food and drink containing the composition will be described. In addition, in the following explanation, when “Χ ～ Υ” (X, 数字 is any number), unless otherwise stated, it is intended to be “X or more and」 or less ”,“ greater than X and smaller than Υ The intention of “preferably” is also included. [0019] The metabolism promoting composition of the present invention can be prepared by incorporating theanine as an active ingredient. These compositions can be incorporated into foods and beverages. Such metabolism promoting compositions and foods and drinks containing the same can be prepared by adding theanine. However, compositions containing theanine, plant extracts, foods and drinks and other theanine-containing substances It can also be prepared by blending.

[0020] Theanine is a derivative of glutamic acid contained in green tea and the like. As an active ingredient of the present invention, for example, L-glutamic acid ethylamide (L-theanine), L-glutamic acid __ methyl amide, D-glutamic acid __ Derivatives containing L- or D-glutamic acid mono-alkyl amides, L- or D-glutamic acid _ -alkyl amides in the basic structure (eg L-amino acid (D-theanine), D-glutamic acid _ _methyl amide, etc.) _ Or D-glutamic acid: a glycoside of Γ_alkyl amide, etc.) and a mixture of one or more compounds selected from the group consisting of two or more compounds can be used. Of these, L-theanine is not only obtainable from natural products, but is also recognized as a food additive, and is particularly preferred from the standpoint of availability and safety.

[0021] Theanine can be produced by various known methods. For example, it can be biosynthesized by a culture method of plants or microorganisms, extracted from tea leaves, fermented or chemically synthesized. Specifically, JP-A-05-068578 (paragraphs [0006]-[0021]), JP-A-5-328986 (paragraphs [0008]-[0027]), JP-A-09-263573 (paragraphs [0009]-[0029]). ]), JP-A-11-225789 (paragraph [000 7]-[0021]), JP-A 2000-26383 (paragraph [0006] _ [002 0]), JP-A-2001_278848 (paragraph [0011]- [0021]), JP 2003-267867 (paragraphs [0005]-[001 7]), JP 2004-01 0545 (paragraphs [0006]-[0036]), JP 2006-0831 5 5 (paragraph [0009] ] _ [0021]) and the like. However, it is not necessarily limited to these manufacturing methods. Also, The obtained theanine may be used as it is, or may be used after purification, or a mixture of both.

[0022] In the present invention, theanine can be used alone as an active ingredient of the metabolism-promoting composition of the present invention, but can also be used by mixing with other components. Theanine can also be used as a lysate in purified products, crude products, tea extract extracts, purified water or physiological saline.

[0023] Metabolism in the present invention generally means a chemical reaction in a living body, and refers to synthesizing another substance or obtaining energy from a substance taken from outside the body. In the present invention, metabolism promotion (activity metabolism) refers to promotion of energy metabolism and does not include drug metabolism. There are two types of energy metabolism: energy consumed by moving the body (activity metabolism) and energy consumed without moving the body and doing nothing (basal metabolism). There is. In the present invention, the promotion of metabolism means that the promotion of metabolism is promoted by promoting the utilization of lipids of theanine which is an active ingredient.

[0024] In the present invention, "improve endurance" means improving endurance and suppressing fatigue in a broad sense of exercise, such as work that requires endurance and labor that requires repeated muscle work. It has the effect | action which carries out. The metabolism promoting composition in the present invention is a composition containing theanine as an active ingredient and having such an action.

[0025] In the present invention, "reducing fatigue during aerobic exercise" means exercise that requires endurance, such as marathon, swimming, and air mouth bicus, and further work in daily life, such as oxygen consumption in the muscles and the cardiopulmonary system. This means that the oxygen supply by the body is in an equilibrium state and has the action of reducing physical fatigue that occurs when performing so-called aerobic exercise. Fatigue is generally broadly divided into physical fatigue and mental fatigue. The composition of the present invention reduces physical fatigue caused by aerobic exercise, and is directly applied only to spiritual fatigue. It does not work. However, in the case of both physical fatigue and mental fatigue, it is necessary to administer the metabolism promoting composition of the present invention. Needless to say, physical fatigue can be reduced.

In the present invention, an increase in the anaerobic metabolic threshold means an increase in the value of oxygen intake at the time when lactic acid begins to accumulate in the blood in the body. Lactic acid is used as a source of energy for skeletal muscle and is therefore an important recovery factor during fatigue. In addition, its importance has been pointed out in the brain and heart ("Separate volume ■ History of medicine: Fatigue chemistry", p. 14, p. 205). However, excessive accumulation of lactic acid in the muscle causes performance degradation by reducing muscle activity. Therefore, suppressing the accumulation of lactic acid in the muscle is important in preventing fatigue.

[0027] Anaerobic threshold metabolism (anaerobic threshold, AT value) is defined as “the value of oxygen intake at the time when lactate begins to accumulate in the blood” (“Introduction to Exercise Testing”) 1 48, 2001). The anaerobic threshold metabolic value was originally proposed by Wasserman, and is currently used as a standard in exercise testing combined with breath gas analysis.

[0028] In increasing load exercise that gradually increases exercise intensity, ventilation (pulmonary respiration) and blood lactate concentration gradually increase at the initial stage. From aerobic exercise to anaerobic exercise at a specific stage. Because it changes, ventilation (pulmonary respiration) and blood lactate concentration increase rapidly. This inflection point, the exercise intensity at the boundary between anaerobic and aerobic exercise, is called the anaerobic metabolic threshold or anaerobic metabolic threshold. In other words, the anaerobic threshold metabolic value is a numerical value indicating how high intensity exercise is possible, and is an index of aerobic endurance. The metabolism promoting composition in the present invention refers to a composition containing theanine as an active ingredient and having such an action.

[0029] In the present invention, blood lactate accumulation reduction refers to an action of suppressing blood lactate accumulation, and such blood lactate accumulation reduction also includes an action of reducing blood lactate. . As mentioned above, lactic acid is considered to be a factor for fatigue recovery of muscles and the like, and the efficient use of lactic acid is desirable from the viewpoint of recovery from fatigue. If this accumulation of lactic acid is suppressed, it is possible to suppress muscle activity decline and exercise more intensely. Can do.

[0030] In the present invention, growth hormone refers to a hormone secreted from GH-secreting cells in the anterior pituitary gland under the action of growth hormone-releasing hormone (GHRH) secreted from the hypothalamus. Growth hormone is involved in the growth of the body and controls all metabolic mechanisms in the body, such as protein synthesis, nitrogen balance, carbohydrate metabolism, fat metabolism, and calcium homeostasis. Carbohydrate metabolism refers to the regulation of blood sugar levels. An excessive increase in blood glucose level is not preferable because it may cause diseases such as hyperglycemia and diabetes. However, when the blood glucose level falls, it causes a feeling of spiritual frustration (feeling irritated, etc.), and when it falls excessively, it causes a lot of cold, sweat, palpitation, hand tremors, etc., resulting in death. There is also. In addition, a decrease in blood glucose level results in fatigue due to inability to perform physical activities. Therefore, it is necessary to maintain an appropriate blood glucose level through growth hormone. In the present invention, the metabolism-promoting progenitor composition refers to a substance that promotes the secretion of growth hormone in vivo.

[0031] In the present invention, the blood glucose level refers to the concentration of glucose in the blood. The blood glucose level is adjusted to a normal value within a very narrow range by the action of insulin that lowers blood sugar and the action of glucagon that raises blood sugar under normal conditions. When blood glucose levels rise after a meal, glucose passes through the GLUT 2 or GLUT 1 transporter; it flows into S cells. When glucose is converted to glucose 6-phosphate by the action of glucokinase, calcium ions flow into the cell and insulin is released. In healthy chicks, the fasting blood glucose level is approximately 80 _ 1 OO mg / d I, which is said to be slightly higher after meals. An excessive rise in blood glucose level is not preferable because it causes diseases such as hyperglycemia and diabetes. However, if the blood glucose level is excessively lowered, it becomes hypoglycemic and causes a feeling of mental agitation (irritability, etc.). In addition, a decrease in blood glucose level results in fatigue and a lack of physical activity. Therefore, it is necessary to maintain an appropriate blood sugar level. In the present invention, the metabolic promoter composition refers to the blood glucose level in vivo. A substance that promotes ascent.

[0032] In the present invention, fat burning mainly refers to a body fat burning action that burns fat stored in the body, but also includes an action of suppressing fat accumulation by inhibiting fat absorption in the body. It is a waste. By ingesting the metabolism-promoting composition of the present invention, theanine promotes the utilization of lipids in the living body, thereby obtaining body fat burning action and fat accumulation inhibiting action.

[0033] In the present invention, anti-fatigue refers to occurrence of physical fatigue, recovery of physical fatigue, and maintenance / improvement of endurance. By ingesting the metabolism promoting composition of the present invention, theanine promotes utilization of lipids and carbohydrates in the living body, and thus has anti-fatigue action, fatigue recovery action, endurance maintenance action, endurance improvement action, nourishing tonic The effect is obtained

[0034] The metabolism promoting composition of the present invention can be provided as a pharmaceutical and a quasi-drug.

[0035] As its form, it may be dried by freeze-drying or spray-drying, etc. and provided as a dry powder, or it may be a liquid, tablet, powder, granule, dragee, capsule, suspension, emulsion, ampoule, injection It can be prepared and provided in any form. When provided as a pharmaceutical product, for example, the active ingredient can be prepared by dissolving it in purified water or physiological saline as it is. When it is provided as a quasi-drug, it is preferably provided in a form that is easy to ingest as much as possible, such as a drinking form such as a container-packed drink, or a form such as a tablet, force capsule, or granule.

[0036] The metabolism promoting composition of the present invention can also be added to food and drink materials. In the present specification, food and drink are intended for beverages and foods.

[0037] Such foods and drinks can be provided as health foods, health drinks, foods for specified health use, functional foods. In that case, it can be prepared by adding the metabolism-promoting composition of the present invention to food materials that are usually blended to produce each food and drink. In addition, when approved as food for specified health use, etc., these foods and drinks shall contain a label indicating that they contain theanine and are used to promote metabolism. It can also be sold as a fresh food or beverage.

[0038] For example, the active ingredient of the present invention may be a variety of food materials (including fruits and jelly), milk ingredients, carbonic acid, excipients (including granulating agents), diluents, or even sweeteners, flavors, In addition to one or more selected from various proteins such as wheat flour, starch, sugar, fats and oils, carbohydrate raw materials, vitamins, minerals, other physiologically active ingredients, hormones, nutritional ingredients, sports drinks, fruits Beverages, tea beverages, vegetable drinks, milk beverages, alcoholic beverages, jelly beverages, carbonated beverages and other beverages, jelly, chewing gum, chocolate, ice cream, candy, biscuits and other sweets, snacks, bread, cakes, etc. Starch-based processed foods, fish paste products, livestock meat products, tofu, cheese and other protein-based processed foods, miso soy sauce, dore Seasonings such as single, other supplements, fodder, can be provided in the form of various food such as Bae Ttofu one de.

[0039] The amount of theanine in the composition and food and drink is not particularly limited as long as it does not impair the intended effect of the present invention. For example, 0.5 mg to 5 mg of theanine per 1 kg of the body weight of the administration subject. Preferably, 0.5 mg to 2 mg of theanine is administered per kg of the subject's body weight. For example, assuming that the composition of the present invention is administered to an adult and the body weight is 40 kg to 1 OO kg, it is preferable to administer 2 Omg to 50 Omg of theanine, and 20 mg to 20 Omg. More preferably, theanine is administered. In other words, it is preferable to appropriately adjust the amount of theanine in the composition and food and drink so that the amount of theanine can be ingested.

[0040] For example, when it is taken as a guide for a person weighing 60 kg, it is preferable to adjust the theanine content to 3 mg to 300 Omg, and in particular, to adjust the theanine content to 3 mg to 12 Omg. Further preferred.

Example

[0041] Hereinafter, the present invention will be described more specifically with reference to examples. However, the present invention is not limited to the following examples.

[0042] (Example 1): Theanine promotes lipid utilization In the experiment, male mouse CDF 1 (10 weeks old, 20 animals) was used. Theanine was prepared by dissolving L-theanine in pure water to prepare a theanine solution.

[0043] In the theanine administration group (10 animals), the mouse was exercised for 180 minutes in a rotating basket, exercised on the mouse, rested for 5 minutes, and then 0.5 mg / kg of theanine was applied. After intraperitoneal administration, the patient was rested for 30 minutes after administration, and then spontaneously exercised again in a rotating basket for 180 minutes.

[0044] On the other hand, in the control administration group (10 animals), the mice were spontaneously operated in a rotating basket for 180 minutes and subjected to exercise load on the mice, then rested for 5 minutes and then administered intraperitoneally. He rested for 30 minutes, and then moved again for 80 minutes in a rotating basket.

[0045] For each administration group, the first exercise amount (number of rotations) and the second amount of exercise (number of rotations) were measured, and heart blood was collected immediately after each exercise to determine the free fatty acid concentration in the blood. It was measured. Figure 1 shows a comparison of the relative momentum between the control administration group (DW) and the theanine administration group (theanine). Regarding the blood fatty acid concentration, the control administration group (DW) and the theanine administration group (theanine) Fig. 2 shows the result of comparison with i ne).

[0046] (Experimental procedure)

1st exercise (rotary motion 1800 minutes) → intraperitoneal administration → break 30 minutes → 2nd exercise (rotary motion 1800 minutes)

[0047] (Result ■ Consideration)

In the control group, the amount of exercise for the second time was suppressed to 20% or less, but in the theanine group, it was maintained at 50% or more. On the other hand, the free fatty acids in the blood were clearly higher in the theanine administration group, confirming that lipid utilization was promoted. Therefore, administration of theanine can promote the breakdown of triglycerides and increase the amount of free fatty acids in the blood, increasing the amount of free fatty acids in the blood supplied to cells as an energy source. Thus, it was found that lipid utilization can be promoted. (Example 2): Theanine's action to reduce body fatigue ■ Endurance improving action Male mice CD F 1 (6 weeks old, 4 animals) were used in the experiment. For theanine, L-theanine was dissolved in pure water to prepare a theanine solution with a theanine concentration of 0.05 mg / m I, and this was used as a sample.

[0049] In the theanine administration group (4 animals), after exercising for 80 minutes in a rotating basket and applying exercise to the mouse, resting for 5 minutes and then orally administering theanine 0.5 mg / kg. After the administration, the patient was rested for 30 minutes, and then spontaneously exercised again in a rotating basket for 180 minutes.

[0050] On the other hand, in the control administration group (4 animals), the mice were exercised for 180 minutes in a rotating basket and subjected to exercise load, and then rested for 5 minutes, and then pure water 1 Om I / kg was orally administered. After administration, the patient was allowed to rest for 30 minutes, and then again exercised again for 180 minutes in a rotating basket.

[0051] For each administration group, the number of rotations of the rotating cage during the first exercise and the number of rotations of the second time were measured, and the number of rotation reductions (times) was determined. The results of comparing the control administration group (water administration group) and the theanine administration group are shown in FIG.

[0052] (Experimental procedure)

1st exercise (rotation 1 80 minutes) → Oral administration → break 30 minutes → 2nd exercise (rotation 1 80 minutes)

[0053] (Result)

In the water-administered group, a decrease in rotation speed of 700 times or more was observed, but in the theanine-administered group, the second rotation speed was rather increased. From this, it was clarified that theanine improves endurance at the time of physical fatigue and exerts an effect of reducing physical fatigue.

[0054] (Example 3): Confirmation of effective concentration of theanine

Male mice CD F 1 (7 weeks old, 7 mice) were used in the experiment. For theanine, L-theanine was dissolved in pure water to prepare theanine solutions with theanine concentrations of 0.02 mg / ml, 0.05 mg I, 0.15 mg / ml and used as samples.

[0055] After one week of preliminary breeding, a selection test was conducted. In this selection test, 1 80 minutes Times

A self-exercising exercise was performed with a rolling basket, and exercise load was applied. After resting for 5 minutes, water was administered orally. After the administration, the patient was rested for 30 minutes, and then spontaneously exercised again in a rotating basket for 180 minutes. The number of rotations of the rotating cage during the first exercise and the number of rotations of the second time were measured.

As selection criteria, individuals with a difference of –300 times or more and 2000 times or less from the second momentum (number of revolutions) minus the first momentum (number of revolutions) were selected.

[0056] In this study, selected individuals (28 animals) were subjected to the same protocol as the selection test, except that theanine was orally administered instead of water. However,

The control group received water. Theanine dose is about 3 at a common ratio of 0.

2 mg / kg, 0.5 mg / kg, 1.5 mg / kg.

[0057] The difference obtained by subtracting the first momentum (number of revolutions) from the second momentum (number of revolutions)

Fig. 4 shows a graph showing the number of rotation reductions for each administration group as the number of rotation reductions (times).

[0058] (Experimental procedure)

[0059] (Result)

In all theanine-administered groups, an effect of suppressing the decrease in rotation speed was observed. From this, by administering at least 0.2 mg / kg or more of theanine, it became clear that the endurance at the time of physical fatigue was improved and the physical fatigue was reduced.

[0060] (Example 4): Endurance enhancing action of theanine

The endurance improvement effect of theanine was investigated by forced swimming test. Male mice CDF 1 (6 weeks old, 8 animals) were used in the experiment. For theanine, L-theanine was dissolved in pure water to prepare theanine solutions having theanine concentrations of 0.02 mg / ml, 0.05 mg / ml, and 0.15 mg / ml, and used as samples.

[0061] After two days of training, selection tests were conducted three times every other day. In the selection test Was placed in a flowing water tank (flow rate 8 L / min, water temperature 34 ° C, average flow rate set to 23-24 cm / sec) and allowed to swim, and the limit swimming time was measured. At this time, dummy mice were used for the test so that there were always 6 mice swimming in the water tank. The selection criteria were that the swimming time SD was within 14 minutes. In addition, a continuous randomization method was used for grouping.

[0062] In this study, selected mice (8 animals in each group) were used, and each mouse was fasted overnight (about 15 hours), then theanine or water was administered, and then left still for 60 minutes after After that, the test was performed using the same protocol as the above selection test, and the swimming time was measured. The results are shown in FIG. The doses of theanine were 0 • 2 mg / kg, 0.5 mg / kg, and 1.5 mg / kg at a common ratio of about 3. Table 1 shows the initial values for each treatment group.

[0063] [Table 1]

(default value)

[0064] (Result)

In all theanine administration groups, the swimming time was prolonged. The results indicate that theanine improves endurance even when not fatigued. It was also found that the effect was exhibited at least 0.2 mg / kg or more.

[0065] (Example 5): Theanine reduces body fatigue

The fatigue reduction effect of theanine was investigated by fatigue load experiment by forced walking

[0066] Male mice CD F 1 (6 weeks old, 5 mice) were used in the experiment. For theanine, L-theanine was dissolved in pure water to prepare theanine solutions with theanine concentrations of 0.05 mg / ml, 0.5 mg / ml, and 5. Omg / ml, and used as samples.

[0067] After one week of training, a selection test was conducted. In the selection test, after 90 minutes of forced walking at a speed of 15 revolutions per minute (hereinafter referred to as 15 rpm), 90 The amount of locomotor activity per minute was measured. This selection test was conducted twice per animal. The selection criteria satisfy the following conditions.

(1) The average of the two momentums is within the overall average soil dispersion.

(2) The absolute value of the difference between the two momentum is smaller than the total variance.

[0068] In this study, selected individuals (5 animals per group) were used as subjects, and theanine or water was forcibly administered orally (5 ml / kg), and then immediately at a rate of 15 rpm. Forced walking was performed for 1 minute, then theanine or water was again administered by oral gavage (5 mL / kg), and then forced walking was performed at a rate of 15 rpm for 45 minutes. Spontaneous exercise was measured for 90 minutes after forced walking. The outline of the experimental procedure is shown in Fig. 6, the initial values are shown in Table 2, the spontaneous exercise amount (times) for each administration group is shown in Fig. 7, and the spontaneous exercise amount in each administration group with respect to the theanine dose is shown in Fig. 8. Indicated. Theanine as the sample to be applied is 0.5 mg / kg at a common ratio of 10 and 5. Omg / kg, 50 mg / kg 7 ”

[0069] [Table 2]

(default value)

(Average person S.D.)

[0070] (Result)

Spontaneous exercise increased in all theanine administration groups. From this, it was revealed that the theanine dose up to 50 mg / kg is effective in reducing physical fatigue. From this result, it was found that the effective dose of theanine was calculated to be 0.1 to 100 mg / kg, and 6 to 600 Omg (as 6 Okg) in human terms.

[0071] (Example 6): Reducing body fatigue by ingestion of theanine

The effects of theanine intake on body fatigue were examined. For evaluation of body fatigue reduction, analysis of components that rise due to fatigue load in blood and breath gas analysis were performed.

[0072] As test beverages, (1) drink 1 (placebo), (2) drink 2 (tea (3) Drink 3 (Theanine high capacity 15 Omg / 2 O Oml), (4) Drink 4 (Catechin 100 mg Zm200 ml). '

[0073] Twelve healthy male university students (average age 20. 0 ± 0. 9 years old) were subjects, and the above beverages were ingested two times a day (morning and evening) one week before the test. On the day of the test, measurements were taken before and after the exercise test. Before and after exercise test, subjective symptoms (VAS), expired gas analysis (AT value, etc.), blood components (acid value, total ketone body, etc.)

) Was measured. The schedule for the day of the test is shown below.

[0074] [Table 3]

Subjects: Healthy H child large ^: 12 (average Φ age 20.0 ± 0.9 years)

Test drink: drinkl (placebo).

drink2 (tea: low for fi3Qmg / 200ml)

drink3 (Thea high for fi 0mg / 200ml)

driuk4 (catechin lOOmgteOOml)

* Top 15 drinking courses were given OTl for 1 to 2 bottles (morning and evening) from the week before the test.

4 groups crossover

[00753

Blood lactate levels at low and high theanine doses decreased. In recent years, it has been reported that lactic acid is an important factor during fatigue, so the decrease in lactic acid levels observed in the examples is thought to be the result of lactic acid contributing to recovery from fatigue.

[0076] FIG. 9 shows AT values measured after ingestion of each test beverage for one week. In the theanine low-dose and high-dose intake groups, an increase in AT value was observed, and the exercise intensity that can be achieved by aerobic exercise increased.

[0077] FIG. 10 shows the blood lactate level (Delda value) before and after the multi-stage exercise test. Blood lactate levels at low and high theanine doses decreased. In recent years, it has been reported that lactic acid is an important cause of fatigue, so the decrease in lactic acid levels observed in the examples is thought to be the result of lactic acid contributing to recovery from fatigue.

(Example 7): Growth hormone secretion action and blood glucose level raising action

Replacement paper (Rule 26) (Experimental animals and rearing conditions)

Wistar male rats (Shizuoka experimental animals) were purchased at 7 weeks of age (approximately 250 g), preliminarily raised for one week, and then subjected to experiments. The animal house is managed in a breeding room with a room temperature of 24 ± 0.5 ° C, humidity of 55 ± 5%, and a light / dark cycle (between 7:00 and 19:00). Water and food (MF, manufactured by Oriental Yeast Co., Ltd.) ).

[0079] (Local hippocampal administration and osmotic pump implantation)

Under anesthesia with pentobarbital, the rat was fixed to a rat stereotaxic apparatus and the skull was exposed. Anchor screws were embedded in the skulls in the frontal and occipital areas, and small holes were made in the skull on the hippocampus according to the rat brain chart (by G. Paxinos and C. Watson). The tip of the drug injection neulet was located from the bregma at P = _5.5, L = ± 4.5, V = 5. Omm. The osmotic pump (Alzet), which was fixed in the skull with dental cement and embedded in the back of the back, was inserted into the hippocampus on both sides.

2002 Durect) were connected with a vinyl tube. The fill v olume of the osmotic pump was 21 4 μ. I, and the pumping rate was 0.5 μ. I / h.

[0080] (Drug adjustment and dosage)

Theanine (manufactured by ITO EN) was dissolved in physiological saline, filtered through a Millipore filter (Millex-GS, manufactured by Millipore), and then filled into an osmotic pump. The drug concentrations were 0.1 u M 1 M and 10; UM, and physiological saline was administered as a control group.

[0081] (Stress load due to fasting)

The rat hippocampus was started with local theanine administration and fasted for about 36 hours from day 9. Thereafter, blood obtained by blood collection was used for analysis.

[0082] (Measurement of blood glucose level and growth hormone)

The blood sugar level at the time of slaughter was measured by the Medice Freeder (GR-101, TERUMO) equipped with a Medisafe chip (MS-FG25, TERUMO Co.).

Co.). Growth hormone was measured by SRL. Non-patent literature (Naoko Ishiwata et al., Hormone) And clinical studies, 38 (6), 601-606), which label antibodies with isotopes.

assay (IRMA).

[0083] (Result)

1; UM or 1 O M theanine was administered directly to the hippocampus to promote growth hormone secretion, especially 1; In addition, the blood glucose level was also examined, and it was found that administration of theanine had the effect of increasing the blood glucose level. Fig. 11 shows the results of examining the amount of growth hormone secreted in plasma, and Fig. 12 shows the results of examining the amount of blood glucose produced.

Brief Description of Drawings

[0084] Fig. 1 is a graph comparing the control exercise group (DW) and the theanine administration group (theanine) regarding the relative momentum in the lipid utilization promotion test of theanine (Example 1).

FIG. 2 is a graph comparing the control fatty acid group (DW) and the theanine administration group (theanine) with respect to blood fatty acid concentration in the lipid utilization promotion test for theanine (Example 1).

[Fig. 3] Reduction of the physical fatigue of theanine ■ In the endurance improvement test (Example 2), the number of rotation reduction (times) is a graph comparing the control administration group (water administration group) and the theanine administration group. is there.

FIG. 4 is a graph showing the number of reductions in rotations (times) for each administration group in the effective concentration confirmation test for theanine (Example 3).

FIG. 5 is a graph showing swimming time (seconds) for each administration group in the endurance improvement test (Example 4) of theanine.

FIG. 6 is a diagram showing an outline of an experimental procedure in the body fatigue reducing action (Example 5) of theanine.

FIG. 7 is a graph showing the amount of spontaneous exercise (times) for each administration group in the body fatigue alleviating action (Example 5) of theanine.

[Fig. 8] In the body fatigue alleviation effect (Example 5) of theanine, It is the graph which showed the spontaneous exercise amount for every administration group with respect to.

FIG. 9 is a diagram showing AT values measured after intake of each test beverage for one week. The AT values measured after ingestion of each test drink for 1 week are shown. An increase in AT was observed in the low and high dose theanine groups, and the exercise intensity that can be achieved with aerobic exercise increased.

FIG. 10 is a graph showing changes in blood lactic acid levels by a multi-stage exercise load test. A treadmill was used for the exercise load. At the start of exercise, a multi-stage exercise load test was conducted with a rotational speed of 0.5 km / h and a slope of 0%, and the rotational speed was increased by 0.5 km / h and the slope by 0.5% every 30 seconds. .

FIG. 1 1 is a diagram showing the results of examining the amount of growth hormone secreted in plasma.

FIG. 12 is a view showing the results of examining the secretion amount of blood glucose level.

Claims

The scope of the claims

[1] A metabolism promoting composition containing theanine as an active ingredient.

[2] The metabolism promoting composition according to [1], wherein the theanine has a lipid utilization promoting action to produce a metabolic promoting action.

[3] Metabolic promotion effects from endurance improvement, growth hormone secretion promotion, blood sugar level increase, fat burning, fatigue reduction during aerobic exercise, anaerobic metabolic threshold increase, blood lactate accumulation reduction, anti-fatigue 3. The composition for promoting metabolism according to claim 1, wherein the composition is selected from the group consisting of:

[4] The metabolism promoting composition according to any one of [1] to [3], comprising 0.5 to 5 mg / kg of theanine per 1 kg of body weight.

[5] The metabolism promoting composition according to any one of [1] to [4], which contains 6 to 600 mg of theanine.

[6] A food or drink comprising the metabolism promoting composition according to any one of claims 1 to 5.

[7] A method for promoting metabolism, comprising orally ingesting the composition for promoting metabolism according to any one of [1] to [6].

[8] Metabolic promotion effects from lipid utilization promotion, growth hormone secretion promotion, blood sugar level increase, fat burning, fatigue reduction during aerobic exercise, anaerobic metabolic threshold increase, blood lactate accumulation reduction, anti-fatigue 8. The method for promoting metabolism according to claim 7, wherein the action is one or more selected from the group consisting of:

9. The method of promoting metabolism according to claim 7 or 8, wherein the theanine is administered in an amount of 0.5 to 5 mg / kg body weight per kilogram per administration.