WO2008009909A1 - Ptéridimones en tant que modulateurs de la kinase de type polo - Google Patents

Ptéridimones en tant que modulateurs de la kinase de type polo Download PDF

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WO2008009909A1
WO2008009909A1 PCT/GB2007/002672 GB2007002672W WO2008009909A1 WO 2008009909 A1 WO2008009909 A1 WO 2008009909A1 GB 2007002672 W GB2007002672 W GB 2007002672W WO 2008009909 A1 WO2008009909 A1 WO 2008009909A1
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optionally substituted
group
alkyl
compound
optionally
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PCT/GB2007/002672
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Iain Simpson
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Astrazeneca Ab
Astrazeneca Uk Limited
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D475/00—Heterocyclic compounds containing pteridine ring systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents

Definitions

  • the present invention relates to pyrimidine derivatives, a process for their preparation, pharmaceutical compositions containing them, a process for preparing the pharmaceutical compositions, and their use in therapy and the treating of conditions mediated by polo-like kinases.
  • Cyclin dependent kinase family have long been considered the master regulators of the cell cycle but an increasing number of diverse protein kinases are emerging as critical components of cell cycle progression. Among these are the polo-like kinase family (Plks), serine/threonine kinases that play multiple roles in regulating progress through cell cycle. In man, four distinct family members have been identified. These are PUtI, Plk2 (Snk), Plk3 (Fnk, Prk) and Plk4 (Sak).
  • Plkl The best characterized family member is Plkl which is conserved from yeast to man and has been implicated in numerous mitotic processes including activation of Cdc25C and Cdkl/Cyclin B at the G2-M transition, centrosome maturation, spindle formation and assembly (Glover et al. 1998, Genes Dev. 12:3777-87; Barr et al 2004, Nat. Rev. MoI. Cell Biol 5:429-441). In the later stages of mitosis Plkl is involved in separation of sister chromatids, activation of components of the anaphase-promoting complex and septin regulation during cytokinesis (van Vugt & Medema 2005, Oncogene 24:2844-2859).
  • Plkl is over-expressed in a broad spectrum of cancer types including breast, colorectal, endometrial, oesophageal, ovarian, prostate, pancreatic, non small cell lung cancers and melanomas (Wolf et al. 1997, Oncogene 14:543-549; Knecht et al. 1999, Cancer Res. 59:2794-2797; Wolf et al. 2000, Pathol. Res. Pract. 196:753-759; Takahashi et al. 2003, Cancer Sci. 94:148-152). The expression of Plkl often correlates with poor patient prognosis.
  • PIk 1 inhibition has been demonstrated in studies employing both antisense oligonucleotides (ASO) and small molecule RNA (siRNA). Reduction in the level of PIk 1 results in the inhibition of proliferation of tumour cells and loss of cell viability both in vivo and in vitro but does not inhibit proliferation of primary cells (Spankuch-Schmitt et al 2002, Oncogene 21: 3162-3171; Elez et al 2003, Oncogene 22:69-80). Microinjection of anti-Plkl antibodies induced mitotic catastrophe in HeLa tumour cells.
  • ASO antisense oligonucleotides
  • siRNA small molecule RNA
  • Plk3 also appears to play roles in mitosis, like Plkl it has been reported to phosphorylate Cdc25C, regulate microtubule dynamics and is involved in centrosome function. Over-expression of Plk3 has been observed in both breast and ovarian carcinomas, with little or no expression in adjacent normal tissue. Increased protein level was associated with enhanced mitosis and was significantly linked to reduced median survival time of patients (Weichert et al. 2005, Virchows Arch 446: 442-450; Weichert et al. 2004 Br. J.Cancer 90:815-821).
  • PIk family members should be of therapeutic value for treatment of proliferative disease including solid rumours such as carcinomas and sarcomas and the leukaemias and lymphoid malignancies.
  • PIk inhibitors should be useful in the treatment of other disorders associated with uncontrolled cellular proliferation.
  • Pteridinone derivatives are known from the prior art as active substances with an antiproliferative activity.
  • WO 01/019825 and WO 03/020722 describe the use of pteridinone derivatives for the treatment of tumoural diseases.
  • tumours The resistance of many types of tumours calls for the development of new pharmaceutical compositions for combating tumours.
  • the aim of the present invention is to provide new compounds having an antiproliferative activity. According to a first aspect of the present invention there is provided a compound of formula (I):
  • R 1 , R 2 each independently represents hydrogen, an optionally substituted C ⁇ alkyl group or an optionally substituted C 3-6 cycloalkyl group, or R 1 and R 2 together with the carbon atom to which they are attached form a 3- to 6-membered saturated or unsaturated ring optionally comprising 1 to 2 heteroatoms;
  • R 3 represents hydrogen, an optionally substituted Ci-i 2 alkyl group, an optionally substituted C 2-12 alkenyl group, an optionally substituted C 2-12 alkynyl group, an optionally substituted C6 -14 aryl group, an optionally substituted C 3-12 cycloalkyl group, an optionally substituted C 3 -i 2 cycloalkenyl group, an optionally substituted C 7 .
  • R c , R d each independently represents hydrogen, an optionally substituted C h alky! group or an optionally substituted C 3 . 6 cycloalkyl group, or R c and R d together with the nitrogen atom to which they are attached form a 3- to 7-membered saturated or unsaturated heterocyclic ring optionally comprising 1 to 2 additional heteroatoms;
  • R a represents H or an optionally substituted C ⁇ alkyl group
  • R b represents -L n -R 5 In, or R a and R b together with the nitrogen atom to which they are attached form a 3- to 7-membered saturated or unsaturated heterocyclic ring optionally comprising 1 to 2 additional heteroatoms;
  • R a2 represents H or an optionally substituted C ⁇ alkyl group
  • R b2 represents -L n -R 5 Hi, or R a2 and R b2 together with the nitrogen atom to which they are attached form a 3- to 7-membered saturated or unsaturated heterocyclic ring optionally comprising 1 to 2 additional heteroatoms
  • R a3 represents H or an optionally substituted Cj-ealkyl group
  • R b3 represents
  • R a3 and R b3 together with the nitrogen atom to which they are attached form a 3- to 7-membered saturated or unsaturated heterocyclic ring optionally comprising 1 to 2 additional heteroatoms;
  • R a4 represents -L n -R 5 m ;
  • R a5 represents -L n -R 5 m
  • R a6 represents -L n -R 5 m
  • L represents a linker selected from optionally substituted C 2- ioalkyl, optionally substituted C 2-1 oalkenyl, optionally substituted C6-i 4 aryl, optionally substituted
  • R 5 represents a group selected from among optionally substituted morpholinyl, piperidinyl, piperazinyl, piperazinylcarbonyl, pyrrolidinyl, tropenyl, diketomethylpiperazinyl, sulfoxomorpholinyl, sulfonylmorpholinyl, thiomorpholinyl, azacycloheptyl and -NR 8 R 9 ;
  • R 6 , R 7 each independently represents hydrogen or an optionally substituted Q ⁇ alkyl group
  • R 8 , R 9 each independently represents hydrogen, Chalky., Cs-iocycloalkyl, C 6 -i 4 aryl, -Ci ⁇ alkyl-Ce-uaryl, pyranyl, pyridinyl, pyrimidinyl,
  • X is O, S or H 2 ;
  • Ar represents a 5- or 6-membered aromatic or heteroaromatic ring optionally comprising one or more ring heteroatoms selected from nitrogen, oxygen and sulfur; or pharmaceutically acceptable salts thereof.
  • alkyl group including alkyl groups that are a part of other groups, unless otherwise stated, includes branched and unbranched alkyl groups with 1 to 12 carbon atoms.
  • C 1-12 alkyl groups include methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl and dodecyl groups.
  • propyl, butyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl and dodecyl include all the possible isomeric forms.
  • propyl includes the two isomeric groups n-propyl and iso-propyl
  • butyl includes n-butyl, iso-butyl, sec-butyl and tert-butyl
  • pentyl includes iso- pentyl, neopentyl, etc.
  • alkyl bridge includes branched and unbranched alkyl bridging groups with 1 to 5 carbon atoms, for example methylene, ethylene, propylene, butylene and pentylene bridges. Unless otherwise stated, the terms propylene, butylene and pentylene include all the possible isomeric forms.
  • 1 or 2 C-atoms may optionally be replaced by one or more heteroatoms selected from among oxygen, nitrogen or sulfur.
  • alkenyl groups includes branched and unbranched alkylene groups with 2 to 10 carbon atoms comprising at least one carbon-carbon double bond.
  • Examples of C 2-1 oalkenyl groups include ethenyl, propenyl, butenyl, pentenyl, hexenyl, heptenyl, octenyl, nonenyl and decenyl groups.
  • propenyl, butenyl, pentenyl, hexenyl, heptenyl, octenyl, nonenyl and decenyl also include all the possible isomeric forms.
  • butenyl includes 1-butenyl, 2-butenyl, 3-butenyl, 1 -methyl- 1 -propenyl, l-methyl-2-propenyl, 2-methyl-l -propenyl, 2-methyl-2-propenyl and 1 -ethyl- 1 -ethenyl.
  • O denotes an oxygen atom linked via a double bond.
  • the hydrogen atoms of the alkenyl group may optionally be replaced, for example a trifluoroethylene group is an ethylene group wherein all the hydrogen atoms have been replaced with fluorine atoms.
  • alkynyl groups includes branched and unbranched alkynyl groups with 2 to 10 carbon atoms comprising at least one triple bond.
  • Examples of C 2- ioalkynyl groups include ethynyl, propynyl, butynyl, pentynyl, hexynyl, heptynyl, octynyl, nonynyl and decynyl groups.
  • propynyl, butynyl, pentynyl, hexynyl, heptynyl, octynyl, nonynyl and decynyl also include all the possible isomeric forms.
  • butynyl includes 1 -butynyl, 2-butynyl, 3-butynyl and l-methyl-2-propynyl.
  • alkyl for example -COOMe or -COOEt; or -CONH 2 .
  • AU the hydrogen atoms of the alkynyl group may optionally be replaced.
  • aryl includes aromatic ring systems with 6 to 14 carbon atoms, said aromatic ring systems comprising one or more rings having from 6 to 14 ring atoms wherein at least one ring is aromatic.
  • C ⁇ -waryl groups include phenyl (C 6 ), indenyl (C 9 ), naphthyl (C 1 O), fluorenyl (Ci 3 ), anthracyl (Ci 4 ), and phenanthryl (C 14 ).
  • one or more hydrogen atoms may optionally be replaced by other substituent groups.
  • aryl groups may be substituted by the following substituents groups: OH; NO 2 ; CN; NH 2 ; halogen, for example fluorine or chlorine; optionally substituted C ⁇ oalkyl, for example methyl, ethyl, propyl or CF 3 ; optionally substituted -OCi -3 alkyl, for example -OMe, -OEt, OCHF 2 , or OCF 3 ; -COOH, -COO-Ci- 4 alkyl, for example -COOMe or -COOEt, or -CONH 2 .
  • substituents groups OH; NO 2 ; CN; NH 2 ; halogen, for example fluorine or chlorine; optionally substituted C ⁇ oalkyl, for example methyl, ethyl, propyl or CF 3 ; optionally substituted -OCi -3 alkyl, for example -OMe, -OEt, OCHF 2 , or OCF 3 ;
  • heteroaryl comprising 1 or 2 nitrogen atoms includes heteroaromatic ring systems with 5 to 14 ring atoms, said heteroaromatic ring systems comprising one or more rings having from 5 to 14 ring atoms wherein at least one ring is aromatic and wherein one or two of the ring atoms are replaced by nitrogen atoms the remaining ring atoms being carbon atoms.
  • heteroaryl groups wherein up to two carbon atoms are replaced by one or two nitrogen atoms comprising one ring include pyrrolyl, pyrazolyl, imidazolyl, triazolyl, pyridinyl and pyrimidinyl groups.
  • heteroaryl rings may optionally also be anellated by a further ring, for example a benzene ring.
  • heteroaryl groups wherein up to two carbon atoms are replaced by one or two nitrogen atoms comprising two rings include indolyl, benzimidazolyl, quinolinyl, isoquinolinyl and quinazolinyl.
  • one or more hydrogen atoms may optionally be replaced by other substituent groups.
  • heteroaryl groups may be substituted by the following substituents groups: F; Cl; Br; OH; OMe; Me; Et; CN; NH 2 ; CONH 2 ; optionally substituted phenyl; and optionally substituted heteroaryl, for example optionally substituted pyridyl.
  • cycloalkyl groups includes cycloalkyl groups comprising 1 ring with 3 to 12 carbon atoms.
  • Examples of C 3-12 cycoalkyl groups include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, cyclononyl, cyclodecyl, cycloundecyl and cyclododecyl groups.
  • one or more hydrogen atoms may optionally be replaced by other substituent groups.
  • cycloalkenyl includes cycloalkenyl groups with 3 to 12 carbon atoms comprising one ring, said ring comprising at least one carbon-carbon double bond.
  • C 3-12 cycloakenyl groups include cyclopropenyl, cyclobutenyl, cyclopentenyl, cyclohexenyl, cycloheptenyl, cyclooctenyl, cyclononenyl, cyclodecenyl, cycloundecenyl and cyclododecenyl groups.
  • heterocycloalkyl and heterocycloakenyl include 3- to 12-membered, for example 5-, 6- or 7-membered heterocycles, which may contain 1 to 4 heteroatoms selected from nitrogen, oxygen or sulfur.
  • Heterocycloalkyl denotes a saturated heterocycle
  • heterocycloakenyl denotes an unsaturated heterocycle.
  • heterocycloalkyl or heterocycloakenyl groups examples include tetrahydrofuran, tetrahydrofuranone, gwmm ⁇ -butyrolactone, alpha-pyran, g ⁇ mm ⁇ -pynan, dioxolane, tetrahydropyran, dioxane, dihydrothiophene, thiolan, dithiolan, pyrroline, pyrrolidine, pyrazoline, pyrazolidine, imidazoline, imidazolidine, tetrazole, piperidine, pyridazine, pyrimidine, pyrazine, piperazine, triazine, tetrazine, morpholine, thiomorpholine, diazepan, oxazine, tetrahydro-oxazinyl, isothiazole, and pyrazolidine.
  • heterocycloalkyl or heterocycloakenyl groups may optionally be replaced by other substituent groups.
  • polycycloalkyl unless otherwise stated, includes cycloalkyl groups comprising 3 to 12 carbon atoms and comprising 2 or more rings. Examples of polycycloalkyl groups include optionally substituted, bi-, tri-, tetra- or pentacyclic cycloalkyl groups, for example pinane, 2,2,2-octane, 2,2,1-heptane or adamantane.
  • polycycloalkenyl includes cycloalkenyl groups comprising 7 to 12 carbon atoms and comprising 2 or more rings wherein at least one ring comprises a carbon-carbon double bond.
  • examples of polycycloalkenyl groups are optionally bridged and/or substituted bi-, tri-, tetra- or pentacyclic cycloalkenyl groups, for example bicycloalkenyl or tricycloalkenyl groups having at least one double bond, such as norbornene.
  • spirocycloalkyl unless otherwise stated, includes spirocycloalkyl groups comprising 5 to 12 carbon atoms and comprising 2 or more rings wherein two rings are joined at a spiro carbon centre.
  • spirocycloalkyl groups include spiro[4.4]nonyl and spiro[3.4]octyl.
  • 5- or 6-membered aromatic or heteroaromatic ring optionally comprising one or more ring heteroatoms selected from nitrogen, oxygen and sulfur is a fully unsaturated, aromatic monocyclic ring containing 5 or 6 atoms of which one or more ring atoms is optionally a heteroatom selected from nitrogen, oxygen or sulfur, with the remaining ring atoms being carbon.
  • Examples of a 5- or 6-membered aromatic or heteroaromatic ring optionally comprising one or more ring heteroatoms selected from nitrogen, oxygen and sulfur include furyl, imidazolyl, isothiazolyl, isoxazolyl, oxazolyl, phenyl, pyrazinyl, pyrazolyl, pyridazinyl, pyridyl, pyrimidinyl, pyrrolyl thiazolyl, thienyl and triazolyl rings.
  • 3- to 6-membered saturated or unsaturated ring optionally comprising 1 to 2 heteroatoms includes optionally substituted C 3-6 cylcoalkyl and optionally substituted C 3 .6cylcoalkenyl groups, and optionally substituted 3- to 6-membered heterocylcoalkyl and optionally substituted 3- to 6-membered heterocylcoalkenyl groups each with 1 or 2 heteroatoms.
  • 3- to 7-membered saturated or unsaturated heterocyclic ring optionally comprising 1 to 2 additional heteroatoms includes optionally substituted 3- to 7-membered heterocylcoalkyl and optionally substituted 3- to 7-membered heterocylcoalkenyl groups each with 1 or 2 heteroatoms
  • halogen includes fluorine, chlorine, bromine or iodine.
  • alkyloxy (-OR wherein R is an alkyl), alkenyloxy (-OR wherein R is an alkenyl), alkynyloxy (-OR wherein R is an alkynyl) and cycloalkyloxy (-OR wherein R is a cycloalkyl) denote an -OR group wherein the respective alkyl, alkenyl, alkynyl or cycloalkyl group is as hereinbefore described above.
  • -alkyl-aryl refers to an alkyl group with an aryl substituent.
  • -alkyl-cycloalkyl refers to an alkyl group with a cycloalkyl substituent.
  • — aryl-alkyl refers to an aryl group with an alkyl substituent.
  • R 5 represents a substituted morpholinyl, piperidinyl, piperazinyl, piperazinylcarbonyl, pyrrolidinyl, tropenyl, diketomethylpiperazinyl, sulfoxomorpholinyl, sulfonylmorpholinyl, thiomorpholinyl, or azacycloheptyl
  • substituents may be present and are as defined above for R 8 .
  • AU the groups mentioned in the definition of R 1 to R 9 may optionally be branched and/or substituted.
  • R 1 , R 2 each independently represents hydrogen or an optionally substituted Q- ⁇ alkyl group, or
  • R 1 and R 2 together with the carbon atom to which they are attached form a 3- to 6- membered saturated or unsaturated ring optionally comprising 1 to 2 heteroatoms;
  • R 3 represents hydrogen, an optionally substituted Ci -12 alkyl group, an optionally substituted C 2-1 2alkenyl group, an optionally substituted C 2-12 alkynyl group, an optionally substituted C 6-14 aryl group, an optionally substituted C 3-12 cycloalkyl group, an optionally substituted C 3-12 cycloalkenyl group, an optionally substituted C 7-12 polycycloalkyl group, an optionally substituted C 7- i 2 polycycloalkenyl group, an optionally substituted Cs. ⁇ spirocycloalkyl group, an optionally substituted 3- to 12-membered heterocycloalkyl group comprising 1 or 2 heteroatoms, an optionally substituted 3- to 12-membered heterocycloalkenyl group comprising 1 or 2 heteroatoms or an optionally substituted 5- or 6-membered heteroaryl ring comprising 1, 2 or 3 heteroatoms each independently selected from nitrogen, oxygen or sulphur, or R 1 and R 3 or R 2 and R 3
  • R 4 each independently represent -CN, hydroxy, -NR 6 R 7 , halogen, an optionally substituted Ci- ⁇ alkyl group, an optionally substituted C 3-6 cycloalkyl group, an optionally substituted C 2-6 alkenyl group, an optionally substituted C 2-6 alkynyl group, an optionally substituted C ⁇ alkyloxy group, an optionally substituted
  • C 3 . 6 cycloalkyloxy group, an optionally substituted C 2 - 5 alkenyloxy group, an optionally substituted C 2- 5alkynyloxy group, an optionally substituted C 1-6 alkythio group, an optionally substituted Ci- ⁇ alkylsulfbxo group or an optionally substituted Ci-galkylsulfonyl group; p is 0, 1 or 2;
  • L represents a linker selected from optionally substituted C 2-1 oalkyl, optionally substituted C 2- ioalkenyl, optionally substituted C 6-14 aryl, optionally substituted -C 2-4 alkyl-C 6-14 aryl, optionally substituted optionally substituted C 3-12 cycloalkyl, and optionally substituted heteroaryl comprising 1 or 2 nitrogen atoms; n is 0 or 1 m is 1 or 2
  • R 5 represents a group selected from among optionally substituted morpholinyl, piperidinyl, piperazinyl, piperazinylcarbonyl, pyrrolidinyl, tropenyl, diketomethylpiperazinyl, sulfoxomorpholinyl, sulfonylrnorpholinyl, thiomorpholinyl, azacycloheptyl and -NR 8 R 9 ;
  • R 6 , R 7 each independently represents hydrogen or an optionally substituted group; and
  • R 8 , R 9 each independently represents hydrogen, C h alky., -C 1-4 alkyl-C3 -10 cycloalkyl,
  • the groups R 1 and R 2 may be identical or different and represent hydrogen or a C ⁇ alkyl group optionally substituted by one or more substituents selected from C 1-3 alkyloxy, C ⁇ alkylthio, C 1-S aIlCyI-S(O) 2 , Q.salkylamino and di-(Ci- 3 alkyl)amino.
  • the groups R 1 and R 2 may be identical or different and represent hydrogen or a methyl or ethyl group. In another embodiment, for compounds of the first and second aspects, the groups R 1 and R 2 are different wherein one of R 1 or R 2 represents hydrogen and the other represents a methyl or ethyl group.
  • R 1 and R 2 together represent a 2- to 5-membered alkyl bridge optionally comprising 1 to 2 heteroatoms selected from oxygen or nitrogen and optionally substituted by one or more substituents selected from C 1-3 alkyloxy, C 1-3 alkylthio, C 1-3 alkyl-S(O) 2 , Cwalkylamino and di-(C 1-3 alkyl)amino.
  • R 1 and R 2 together represent an ethylene or propylene bridge.
  • R 3 represents hydrogen; a C 1-12 alkyl, for example ethyl, propyl, butyl, pentyl or hexyl, optionally substituted by one or more substituents selected from C 1-3 alkyloxy, d ⁇ aUcylthio, C 1-3 alkyl- S(O) 2 , Ci -3 alkylamino and di-(C 1-3 alkyl)amino; a C 2-12 alkenyl, for example C 5-7 alkenyl, optionally substituted by one or more substituents selected from C 1-3 alkyloxy, C ⁇ alkylthio, Ci.
  • alkyl-S(O) 2 C 2-12 alkynyl, for example C 5- 7 alkynyl, optionally substituted by one or more substituents selected from C 1-3 allcyloxy, Ci -3 alkylthio, Ci -3 alkyl-S(O) 2 , C 1-3 alkylamino and di-(C 1-3 alkyl)amino; a C ⁇ - ⁇ aryl, for example phenyl, optionally substituted by one or more substituents selected from Ci -3 alkyloxy, Ci- 3 alkylthio, Ci -3 alkyl-S(O) 2 , C 1-3 alkylamino and di-(Ci- 3 alkyl)amino; a C 3- 12 cycloalkyl, for example cyclopentyl or cyclohexyl, optionally substituted by one or more substituents selected from C 1-3 alkylamino and di- (C 1-3 alkyl)amino; a C 3-12
  • 3 alkyl)amino 3-12 membered heterocycloalkyl which contains 1 to 2 heteroatoms selected from oxygen, nitrogen or sulfur, for example pyranyl or piperidinyl, pyrrolidinyl, pyrazinyl or morpholinyl, optionally substituted by one or more substituents selected from C 1-3 alkyloxy, Ci -3 alkylthio, Ci -3 alkyl-S(O) 2 , C ⁇ alkylamino and di-(Ci -3 alkyl)amino; and 3- to 12-membered heterocycloalkenyl which contains 1 to 2 heteroatoms selected from oxygen, nitrogen or sulfur, optionally substituted by one or more substituents selected from C 1-3 alkylthio, C 1-3 alkyl-S(O) 2 , C 1-3 alkylamino and di-(Ci -3 alkyl)amino.
  • R 3 represents isopropyl, isobutyl, isopentyl, cyclopentyl, phenyl or cyclohexyl.
  • R 1 and R 3 or R 2 and R 3 together represent a saturated or unsaturated C 3 -C 4 alkyl bridge optionally comprising 1 heteroatom selected from oxygen or nitrogen.
  • R 4 represents a group selected from among -CN; hydroxyl; -NR 6 R 7 ; halogen, for example chlorine or fluorine; C 1-6 alkyl, for example methyl, ethyl or propyl, optionally substituted by one or more substituents selected from C 1-3 alkyloxy, C 1-3 alkylthio, C 1-3 alkylamino and di-(Ci -3 alkyl)amino; C 2-6 alkenyl, for example ethenyl or propenyl, optionally substituted by one or more substituents selected from C 1-3 alkyloxy, C 1-3 alkylthio, C )-3 alkyl-S(O) 2 , C 1-3 alkylamino and di-(C 1-3 alkyl)amino; C 2-6 alkynyl, for example ethynyl, propynyl or butynyl, optionally substitute
  • R 4 represents methoxy, methyl, ethoxy, ethyl, propargyloxy, chlorine.
  • each R 4 may be the same or different and selected from methoxy, methyl, ethoxy, ethyl, propargyloxy, chlorine or fluorine.
  • both R 4 together with the aromatic ring atoms to which they are attached form a 4- to 7-member unsaturated ring optionally comprising 1 to 2 heteroatoms.
  • L represents a linker selected from among C 2- i 0 alkyl, for example ethyl, propyl, butyl or pentyl, optionally substituted by one or more substituents selected from C ⁇ alkyloxy, C 1-3 alkylthio, C 1 -3alkyl-S(O) 2 , Ci -3 alkylamino and di-(C 1-3 alkyl)amino; C 2-1 oalkenyl, optionally substituted by one or more substituents selected from Ci -3 alkyloxy, C 1-3 alkylthio, Ci -3 alkyl-S(O) 2 , C 1-3 alkylamino and di-(C 1-3 alkyi)animo; C6-i 4 aryl, for example phenyl, optionally substituted by one or more substituents selected from C 1-3 alkyloxy, C 1-3 alkylthio, C 1-3 alkyl-S(O) 2
  • L represents an optionally substituted a C 2- i 0 alkyl linker.
  • L represents -C(CH 3 ) 2 -CH 2 - or -CH 2 -C(CH 3 ) 2 -CH 2 -.
  • n 1
  • R 5 represents a group selected from among optionally substituted morpholinyl, piperidinyl, piperazinyl, piperazinylcarbonyl, pyrrolidinyl, tropenyl, diketomethylpiperazinyl, sulfoxomorpholinyl, sulfonylmorpholinyl, thiomorpholinyl, -NR 8 R 9 and azacycloheptyl wherein each morpholinyl, piperidinyl, piperazinyl, piperazinylcarbonyl, pyrrolidinyl, tropenyl, diketomethylpiperazinyl, sulfoxomorpholinyl, sulfonylmorpholinyl, thiomorpholinyl, -NR 8 R 9 and azacycloheptyl is optionally substituted by one or more groups as defined for R 8 .
  • R 5 represents piperidinyl, morpholinyl, pyrrolidinyl, sulfoxomorpholinyl, piperazinyl, thiomorpholinyl or tropenyl each optionally substituted by one or more groups as defined for R 8 .
  • the groups R 6 and R 7 may be identical or different and represent hydrogen or Ci -4 alkyl, for example methyl or ethyl.
  • the groups R 8 and R 9 may be identical or different and represent hydrogen; a Ci ⁇ alkyl, for example methyl, ethyl or propyl, optionally substituted by one or more substituents selected from C 1-3 alkyloxy, C 1-3 alkylthio, Ci -3 alkylamino and di-(C 1-3 alkyl)amino; for example -CHb-cyclopropyl, optionally substituted by one or more substituents selected from C ⁇ alkyloxy, C ⁇ alkylthio, Ci -3 alkyl-S(O) 2 , C 1-3 alkylamino and di-(C 1-3 alkyl)amino; C 3-10 cycloalkyl optionally substituted by one or more substituents selected from Ci -3 alkyloxy, Ci ⁇ alkylthio, C 1-3 alkyl-S(O) 2 , Ci.3alkylamino and di-(Ci.
  • a Ci ⁇ alkyl for example methyl, eth
  • C 6 .i 4 aryl for example phenyl, optionally substituted by one or more substituents selected from C ⁇ alkyloxy, C 1 . 3 alkyltb.io, C 1-3 alkyl-S(O) 2 , Ci ⁇ alkylamino and di-(Ci.
  • Ci alkylamino and di-(Ci. 3 alkyl)amino
  • pyranyl optionally substituted by one or more substituents selected from Ci ⁇ alkyloxy, C ⁇ alkylthio, C 1- SaIlCyI-S(O) 2 , and di-(C 1- 3alkyl)amino
  • Ci -4 alkyloxycarbonyl optionally substituted by one or more substituents selected from Ci -3 alkyloxy, C 1 .
  • Ci- 3 alkyltb.io Ci- 3 alkyl-S(O) 2 , and di-(Ci,3alkyl)amino
  • C 6 - I4 arylmethyloxycarbonyl optionally substituted by one or more substituents selected from Ci-salkyloxy, Q.salkylthio, Ci -3 alkyl-S(O) 2 , Ci ⁇ alkylamino and di-(C 1-3 alkyl)amino
  • C 6 _i 4 arylsulfonyl optionally substituted by one or more substituents selected from and di-(Ci,3alkyl)amino
  • Ci Ci.
  • R 8 represents methyl, ethyl or propyl.
  • R 9 represents methyl, ethyl or propyl.
  • R° and R each independently represents hydrogen or optionally substituted Ci- 3 alkyl group or an optionally substituted C 3-4 cycloalkyl group.
  • R Q and R d each independently represents hydrogen, methyl or ethyl.
  • R c and R d both represent hydrogen.
  • n 1 and L is an optionally substituted C 2- ioalkyl linker.
  • n is 0 and m is 1.
  • R 1 to R 4 , R 6 and R 7 are as hereinbefore defined; and L represents a linker selected from among optionally substituted C 2- ioalkyl, optionally substituted
  • C 2-1 oalkenyl optionally substituted C 6-14 aryl, optionally substituted -C 2 . 4 alkyl-C 6-14 aryl, optionally substituted -Ce-waryl-C ⁇ alkyl, optionally substituted C3 -12 cycloalkyl and optionally substituted heteroaryl comprising 1 or 2 nitrogen ring atoms; n denotes 1; m denotes 1 or 2; R 5 denotes a group which is bound to L via a nitrogen atom, selected from optionally substituted morpholinyl, optionally substituted piperidinyl, optionally substituted piperazinyl, optionally substituted pyrrolidinyl, optionally substituted tropenyl, optionally substituted d diketomethylpiperazinyl, optionally substituted sulfoxomorpholinyl, optionally substituted sulfonylmorpholinyl, optionally substituted thiomorpholinyl, -NR 8 R 9 and optionally substituted
  • R 5 denotes a group which is bound to L via a carbon atom, selected from among piperidinyl, piperazinyl, pyrrolidinyl, piperazinylcarbonyl, tropenyl, morpholinyl and azacycloheptyl each optionally substituted by one or more groups as defined for R 8 ; and R 8 , R 9 independently represent hydrogen, Ci. 6 alkyl, -C 1-4 alkyl-C 3-1 ocycloalkyl, Ca-iocycloalkyl,
  • R 1 , R 2 , m, n and R 5 to R 8 are as hereinbefore defined; and R 3 represents an optionally substituted C 1-1O aIlCyI, optionally substituted C3 -7 cycloalkyl, optionally substituted C3_ 6 heterocycloalkyl or optionally substituted C 6-14 aryl group or R 1 and R 3 or R 2 and R 3 together represent a saturated or unsaturated bridge optionally comprising 1 or 2 heteroatoms; R 4 represents hydrogen, OMe, OH, Me, Et, Pr, OEt, NHMe, NH 2 , F, Cl, Br, O-propargyl, O-butynyl, CN, SMe, NMe 2 , CONH 2 , ethynyl, propynyl, butynyl and allyl; and L denotes a linker selected from among optional
  • R 1 and R 2 may be identical or different and represent hydrogen or a methyl or ethyl group
  • R 3 represents isopropyl, isobutyl, isopentyl, cyclopentyl, phenyl or cyclohexyl
  • p is 1 or 2
  • R 4 represents methoxy, methyl, ethoxy, ethyl, propargyloxy, fluorine or chlorine
  • m is 0
  • n is 1
  • R 5 denotes a group selected from piperidinyl, morpholinyl, pyrrolidinyl, sulfoxomorpholinyl, piperazinyl, thiomorpholinyl or tropenyl each optionally substituted by one or more groups as defined for R 8
  • R 8 is methyl, ethyl or propyl, or pharmaceutically acceptable salts thereof.
  • a particular compound of the invention is the compound of Examples 1 or the pharmaceutically acceptable salts thereof.
  • references are intended to include tautomers, the individual optical isomers, diastereomers or racemates and mixtures of the individual enantiomers, diastereomers or racemates of the compounds.
  • the compounds according to the first and second aspects of the invention may be present in the form of the individual optical isomers, mixtures of the individual enantiomers, diastereomers or racemates, in the form of the tautomers and also in the form of the free bases or the corresponding salts with pharmaceutically acceptable acids, such as for example acid addition salts with hydrohalic acids, for example hydrochloric or hydrobromic acid, or organic acids, such as for example oxalic, fumaric, diglycolic or methanesulfonic acid.
  • pharmaceutically acceptable acids such as for example acid addition salts with hydrohalic acids, for example hydrochloric or hydrobromic acid, or organic acids, such as for example oxalic, fumaric, diglycolic or methanesulfonic acid.
  • the compounds of formula (I) or (II) above may be converted to a pharmaceutically acceptable salt, for example, an acid addition salt such as a hydrochloride, hydrobromide, phosphate, acetate, fumarate, maleate, tartrate, citrate, oxalate, methanesulfonate or p- toluenesulfonate salt, or a metal salt such as a calcium, magnesium, sodium or potassium salt.
  • an acid addition salt such as a hydrochloride, hydrobromide, phosphate, acetate, fumarate, maleate, tartrate, citrate, oxalate, methanesulfonate or p- toluenesulfonate salt
  • a metal salt such as a calcium, magnesium, sodium or potassium salt.
  • Certain compounds of formula (I) or (II) are capable of existing in stereoisomeric forms. It will be understood that the invention encompasses the use of all geometric and optical isomers (including atropisomers) of the compounds of formula (I) or (II) and mixtures thereof including racemates. The use of tautomers and mixtures thereof also form an aspect of the present invention.
  • the invention also relates to a process for preparing a compound of general formula (H),
  • R 3 (III) wherein R 1 -R 3 , R Q and R d are as hereinbefore defined and A is a leaving group, with an optionally substituted compound of general formula (IV):
  • R 4 is as hereinbefore defined; and R 10 denotes OH, NH-L m -R 5 n , OMe, OEt, and, when R 10 denotes OH, OMe or OEt, optionally after previous hydrolysis of the ester group -COR 10 , reacting with an amine of general formula (VI): NH 2 -L m -R 5 n
  • R 5 , L and m are as hereinbefore defined to give a compound of formula (II).
  • R 10 is a substituent selected from among OH, NH-LR 5 , -O-methyl and -O-ethyl.
  • leaving group includes leaving groups such as for example -O-methyl, -SCN, fluoride, chloride, bromide, iodide, methanesulfonyl, trifluoromethanesulfonyl or p-toluenesulfonyl.
  • the leaving group A is chloride.
  • Aminating reagents are described in Encyclopaedia of Reagents for Organic Synthesis, 1995, VoI 5, 3270-3297 and include the use O-acyl hydroxylamines, such as amino 2,4,6-trimethylbenzoate.
  • Compounds of Formula (III) may also be obtained by the cyclisation of a compound of formula (VII) (VII) wherein R p is hydrogen or a protecting group; and R 1 -R 4 , and A are as hereinbefore defined, for example by palladium coupling, and optionally removing any protecting group.
  • the protection and deprotection of functional groups is described in 'Protective Groups in Organic Chemistry', edited by J.W.F. McOmie, Plenum Press (1973) and 'Protective Groups in Organic Synthesis', 2nd edition, T.W. Greene and P.G.M. Wuts, Wiley Interscience (1991).
  • the invention further relates to compounds of formula (I) or (II) for use as pharmaceutical compositions.
  • compounds of formula (I) or (II) are of use as pharmaceutical compositions with an antiproliferative activity.
  • the invention also relates to the use of a compound of formula (I) or (II) for preparing a pharmaceutical composition for the treatment and/or prevention of cancer, infections, inflammatory and autoimmune diseases.
  • PIk inhibitors should be of therapeutic value for treatment of proliferative disease including solid tumours such as carcinomas and sarcomas and the leukaemias and lymphoid malignancies.
  • PIk inhibitors should be useful in the treatment of other disorders associated with uncontrolled cellular proliferation.
  • One aspect of the current invention therefore relates to the use of one or more of the compounds of formula (I) or (II) in the treatment of disorders characterised by excessive or anomalous cell proliferation.
  • Such diseases include for example: viral infections such as HIV and Kaposi's sarcoma; inflammatory and autoimmune diseases such as colitis, rheumatoid arthritis, Alzheimer's disease, glomerulonephritis and wound healing; bacterial, fungal and parasitic infections such as malaria and emphysema; dermatological diseases such as psoriasis; bone diseases; cardiovascular diseases such as restenosis and cardiomyopathy.
  • viral infections such as HIV and Kaposi's sarcoma
  • inflammatory and autoimmune diseases such as colitis, rheumatoid arthritis, Alzheimer's disease, glomerulonephritis and wound healing
  • bacterial, fungal and parasitic infections such as malaria and emphysema
  • dermatological diseases such as psoriasis
  • bone diseases such as restenosis and cardiomyopathy.
  • cardiovascular diseases such as restenosis and cardiomyopathy.
  • the invention also relates to a method of treating and/or preventing cancer, infections, inflammatory and autoimmune diseases, characterised in that a patient is given an effective amount of a compound of formula (I) or (II).
  • the invention also relates to pharmaceutical preparations, containing as active substance one or more compounds of general fonnula (I) or (II), or the physiologically acceptable salts thereof, optionally combined with conventional excipients and/or carriers.
  • the compounds of formula (I) and (II) have activity as pharmaceuticals, in particular as modulators or inhibitors of PIk activity, and may be used in the treatment of proliferative and hyperproliferative diseases/conditions, examples of which include the following cancers:
  • carcinoma including that of the bladder, brain, breast, colon, kidney, liver, lung, ovary, pancreas, prostate, stomach, cervix, colon, thyroid and skin;
  • hematopoietic tumours of lymphoid lineage including acute lymphocytic leukaemia, B cell lymphoma and Burketts lymphoma
  • hematopoietic tumours of myeloid lineage including acute and chronic myelogenous leukaemias and promyelocytic leukaemia
  • tumours of mesenchymal origin including fibrosarcoma and rhabdomyosarcoma
  • tumours including melanoma, seminoma, tetratocarcinoma, neuroblastoma and glioma.
  • the compounds of formula (I) and (II) are useful in the treatment of tumours of the lung, breast and prostate.
  • the present invention provides a compound of formula (I) or (II), or a pharmaceutically acceptable salt thereof, as hereinbefore defined for use in therapy.
  • a compound of formula (I) or (II), or a pharmaceutically acceptable salt thereof, as hereinbefore defined for use in a method or treatment of the human or animal body by therapy is provided.
  • the present invention provides the use of a compound of formula (I) or (II), or a pharmaceutically acceptable salt thereof, as hereinbefore defined in the manufacture of a medicament for use in therapy.
  • the term “therapy” also includes “prophylaxis” unless there are specific indications to the contrary.
  • the terms “therapeutic” and “therapeutically” should be construed accordingly.
  • the invention also provides a method of treating cancer which comprises administering to a patient in need thereof a therapeutically effective amount of a compound of formula (I) or (II), or a pharmaceutically acceptable salt thereof, as hereinbefore defined.
  • the invention still further provides a method of modulating polo-like kinase (PIk) activity which comprises administering to a patient in need thereof a therapeutically effective amount of a compound of formula (I) or (II), or a pharmaceutically acceptable salt thereof, as hereinbefore defined.
  • PIk polo-like kinase
  • a method for producing a PLK inhibitory effect in a warm-blooded animal, such as man, in need of such treatment which comprises administering to said animal an effective amount of a compound of formula (I) or (II), or a pharmaceutically acceptable salt thereof, as defined herein.
  • a method for producing an anti-cancer effect in a warm-blooded animal, such as man, in need of such treatment which comprises administering to said animal an effective amount of a compound of formula (I) or (II), or a pharmaceutically acceptable salt thereof, as defined herein.
  • composition which comprises a compound of the formula (I) or (II), or a pharmaceutically acceptable salt thereof, as defined herein in association with a pharmaceutically-acceptable diluent or carrier for use in the production of a PLK inhibitory effect in a warm-blooded animal such as man.
  • composition which comprises a compound of the formula (I) or (II), or a pharmaceutically acceptable salt thereof, as defined herein in association with a pharmaceutically-acceptable diluent or carrier for use in the production of an anti-cancer effect in a warm-blooded animal such as man.
  • a pharmaceutical composition which comprises a compound of the formula (I) or (II), or a pharmaceutically acceptable salt thereof, as defined herein in association with a pharmaceutically-acceptable diluent or carrier for use in the treatment of melanoma, papillary thyroid tumours, cholangiocarcinomas, colon cancer, ovarian cancer, lung cancer, leukaemias, lymphoid malignancies, multiple myeloma, carcinomas and sarcomas in the liver, kidney, bladder, prostate, breast and pancreas, and primary and recurrent solid tumours of the skin, colon, thyroid, lungs and ovaries in a warm-blooded animal such as man.
  • the compounds of formula (I) and (II), and pharmaceutically acceptable salts and solvates thereof may be used on their own but will generally be administered in the form of a pharmaceutical composition in which the formula (I) or (II) compound/salt/solvate (active ingredient) is in association with a pharmaceutically acceptable adjuvant, diluent or carrier.
  • the pharmaceutical composition will preferably comprise from 0.05 to 99%w (per cent by weight), more preferably from 0.05 to 80%w, still more preferably from 0.10 to 70% w, and even more preferably from 0.10 to 50%w, of active ingredient, all percentages by weight being based on total composition.
  • the present invention also provides a pharmaceutical composition comprising a compound of formula (I), or a pharmaceutically acceptable salt thereof, as hereinbefore defined, in association with a pharmaceutically acceptable adjuvant, diluent or carrier.
  • the invention further provides a process for the preparation of a pharmaceutical composition of the invention which comprises mixing a compound of formula (I) or (II), or a pharmaceutically acceptable salt thereof, as hereinbefore defined, with a pharmaceutically acceptable adjuvant, diluent or carrier.
  • compositions may be administered topically (e.g. to the skin or to the lung and/or airways) in the form, e.g., of creams, solutions, suspensions, heptafluoroalkane aerosols and dry powder formulations; or systemically, e.g. by oral administration in the form of tablets, capsules, syrups, powders or granules; or by parenteral administration in the form of solutions or suspensions; or by subcutaneous administration; or by rectal administration in the form of suppositories; or transdermally.
  • compositions of the invention may be obtained by conventional procedures using conventional pharmaceutical excipients, well known in the art.
  • compositions intended for oral use may contain, for example, one or more colouring, sweetening, flavouring and/or preservative agents.
  • Suitable pharmaceutically acceptable excipients for a tablet formulation include, for example, inert diluents such as lactose, sodium carbonate, calcium phosphate or calcium carbonate, granulating and disintegrating agents such as corn starch or algenic acid; binding agents such as starch; lubricating agents such as magnesium stearate, stearic acid or talc; preservative agents such as ethyl or propyl p-hydroxybenzoate and anti oxidants such as ascorbic acid. Tablet formulations may be uncoated or coated either to modify their disintegration and the subsequent absorption of the active ingredient within the gastrointestinal tract, or to improve their stability and/or appearance, in either case, using conventional coating agents and procedures well known in the art.
  • inert diluents such as lactose, sodium carbonate, calcium phosphate or calcium carbonate
  • granulating and disintegrating agents such as corn starch or algenic acid
  • binding agents such as starch
  • lubricating agents such as
  • Compositions for oral use may be in the form of hard gelatin capsules in which the active ingredient is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin, or as soft gelatin capsules in which the active ingredient is mixed with water or an oil such as peanut oil, liquid paraffin, or olive oil.
  • an inert solid diluent for example, calcium carbonate, calcium phosphate or kaolin
  • water or an oil such as peanut oil, liquid paraffin, or olive oil.
  • Aqueous suspensions generally contain the active ingredient in finely powdered form together with one or more suspending agents, such as sodium carboxymethylcellulose, methylcellulose, hydroxypropylmethylcellulose, sodium alginate, polyvinyl pyrrolidone, gum tragacanth and gum acacia; dispersing or wetting agents such as lecithin or condensation products of an alkylene oxide with fatty acids (for example polyoxethylene stearate), or condensation products of ethylene oxide with long chain aliphatic alcohols, for example heptadecaethyleneoxycetanol, or condensation products of ethylene oxide with partial esters derived from fatty acids and a hexitol such as polyoxyethylene sorbitol monooleate, or condensation products of ethylene oxide with long chain aliphatic alcohols, for example heptadecaethyleneoxycetanol, or condensation products of ethylene oxide with partial esters derived from fatty acids and a hexitol such as polyoxyethylene sorbitol
  • the aqueous suspensions may also contain one or more preservatives (such as ethyl or propyl p- hydroxybenzoate, anti oxidants (such as ascorbic acid), colouring agents, flavouring agents, and/or sweetening agents (such as sucrose, saccharine or aspartame).
  • preservatives such as ethyl or propyl p- hydroxybenzoate, anti oxidants (such as ascorbic acid), colouring agents, flavouring agents, and/or sweetening agents (such as sucrose, saccharine or aspartame).
  • Oily suspensions may be formulated by suspending the active ingredient in a vegetable oil (such as arachis oil, olive oil, sesame oil or coconut oil) or in a mineral oil (such as liquid paraffin).
  • the oily suspensions may also contain a thickening agent such as beeswax, hard paraffin or cetyl alcohol. Sweetening agents such as those set out above, and flavouring agents may be added to provide a palatable oral preparation. These compositions may be preserved by the addition of an anti oxidant such as ascorbic acid.
  • Dispersible powders and granules suitable for preparation of an aqueous suspension by the addition of water generally contain the active ingredient together with a dispersing or wetting agent, suspending agent and one or more preservatives. Suitable dispersing or wetting agents and suspending agents are exemplified by those already mentioned above. Additional excipients such as sweetening, flavouring and colouring agents, may also be present.
  • the pharmaceutical compositions of the invention may also be in the form of oil in water emulsions.
  • the oily phase may be a vegetable oil, such as olive oil or arachis oil, or a mineral oil, such as for example liquid paraffin or a mixture of any of these.
  • Suitable emulsifying agents may be, for example, naturally occurring gums such as gum acacia or gum tragacanth, naturally occurring phosphatides such as soya bean, lecithin, an esters or partial esters derived from fatty acids and hexitol anhydrides (for example sorbitan monooleate) and condensation products of the said partial esters with ethylene oxide such as polyoxyethylene sorbitan monooleate.
  • the emulsions may also contain sweetening, flavouring and preservative agents.
  • Syrups and elixirs may be formulated with sweetening agents such as glycerol, propylene glycol, sorbitol, aspartame or sucrose, and may also contain a demulcent, preservative, flavouring and/or colouring agent.
  • sweetening agents such as glycerol, propylene glycol, sorbitol, aspartame or sucrose, and may also contain a demulcent, preservative, flavouring and/or colouring agent.
  • compositions may also be in the form of a sterile injectable aqueous or oily suspension, which may be formulated according to known procedures using one or more of the appropriate dispersing or wetting agents and suspending agents, which have been mentioned above.
  • a sterile injectable preparation may also be a sterile injectable solution or suspension in a non-toxic parenterally acceptable diluent or solvent, for example a solution in 1,3-butanediol.
  • Suppository formulations may be prepared by mixing the active ingredient with a suitable non irritating excipient which is solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt in the rectum to release the drug. Suitable excipients include, for example, cocoa butter and polyethylene glycols.
  • Topical formulations such as creams, ointments, gels and aqueous or oily solutions or suspensions, may generally be obtained by formulating an active ingredient with a conventional, topically acceptable, vehicle or diluent using conventional procedure well known in the art.
  • compositions for administration by insufflation may be in the form of a finely divided powder containing particles of average diameter of, for example, 30 ⁇ or much less, the powder itself comprising either active ingredient alone or diluted with one or more physiologically acceptable carriers such as lactose.
  • the powder for insufflation is then conveniently retained in a capsule containing, for example, 1 to 50mg of active ingredient for use with a turbo inhaler device, such as is used for insufflation of the known agent sodium cromoglycate.
  • Compositions for administration by inhalation may be in the form of a conventional pressurised aerosol arranged to dispense the active ingredient either as an aerosol containing finely divided solid or liquid droplets.
  • Conventional aerosol propellants such as volatile fluorinated hydrocarbons or hydrocarbons may be used and the aerosol device is conveniently arranged to dispense a metered quantity of active ingredient.
  • the size of the dose for therapeutic purposes of a compound of the invention will naturally vary according to the nature and severity of the conditions, the age and sex of the animal or patient and the route of administration, according to well known principles of medicine.
  • a compound of the invention will be administered so that a daily dose in the range, for example, from 0.5 mg to 75 mg active ingredient per kg body weight is received, given if required in divided doses. In general lower doses will be administered when a parenteral route is employed.
  • a dose in the range for example, from 0.5 mg to 30 mg active ingredient per kg body weight will generally be used.
  • a dose in the range for example, from 0.5 mg to 25 mg active ingredient per kg body weight will generally be used.
  • a formulation intended for oral administration to humans will generally contain, for example, from 0.5 mg to 2 g of active ingredient.
  • a formulation intended for oral administration to humans will generally contain, for example, from 0.5 mg to 2 g of active ingredient.
  • anti-tumour agents may include one or more of the following categories of anti-tumour agents:-
  • antiproliferative/antineoplastic drugs and combinations thereof, as used in medical oncology such as alkylating agents (for example cisplatin, oxaliplatin, carboplatin, cyclophosphamide, nitrogen mustard, melphalan, chlorambucil, busulfan, temozolamide and nitrosoureas); antimetabolites (for example gemcitabine and antifolates such as fluoropyrirnidines like 5 fluorouracil and tegafur, raltitrexed, methotrexate, cytosine arabinoside, and hydroxyurea); antitumour antibiotics (for example anthracyclines like adriamycin, bleomycin, doxorubicin, daunomycin, epirabicin, idarubicin, mitomycin-C, dactinomycin and mithramycin); antimitotic agents (for example vinca alkaloids like vincristine, vin
  • cytostatic agents such as antioestrogens (for example tamoxifen, fulvestrant, toremifene, raloxifene, droloxifene and iodoxyfene), antiandrogens (for example bicalutamide, flutamide, nilutamide and cyproterone acetate), LHRH antagonists or LHRH agonists (for example goserelin, leuprorelin and buserelin), progestogens (for example megestrol acetate), aromatase inhibitors (for example as anastrozole, letrozole, vorazole and exemestane) and inhibitors of 5*-reductase such as finasteride;
  • antioestrogens for example tamoxifen, fulvestrant, toremifene, raloxifene, droloxifene and iodoxyfene
  • antiandrogens for example
  • anti-invasion agents for example c-Src kinase family inhibitors like 4-(6- chloro-2,3-methylenedioxyanilino)-7-[2-(4-methylpiperazin-l-yl)ethoxy]-5-tetrahydropyran- 4-yloxyquinazoline (AZD0530; International Patent Application WO 01/94341) and N-(2- chloro-6-methylphenyl)-2- ⁇ 6-[4-(2-hydroxyethyl)piperazin- 1 -yl]-2-methylpyriniidin-4- ylamino ⁇ thiazole-5-carboxamide (dasatinib, BMS-354825; J. Med.
  • anti-invasion agents for example c-Src kinase family inhibitors like 4-(6- chloro-2,3-methylenedioxyanilino)-7-[2-(4-methylpiperazin-l-yl)ethoxy]-5-tetrahydropyran
  • inhibitors of growth factor function include growth factor antibodies and growth factor receptor antibodies (for example the anti erbB2 antibody trastuzumab [HerceptinTM], the anti-EGFR antibody panitumumab, the anti erbBl antibody cetuximab [Erbitux, C225] and any growth factor or growth factor receptor antibodies disclosed by Stern et al. Critical reviews in oncology/haematology, 2005, Vol.
  • inhibitors also include tyrosine kinase inhibitors, for example inhibitors of the epidermal growth factor family (for example EGFR family tyrosine kinase inhibitors such as N-(3-chloro-4-fluorophenyl)-7-methoxy-6-(3-mo ⁇ holinopropoxy)quinazolin-4-amine (gef ⁇ tinib, ZD1839), N-(3-ethynylphenyl)-6,7-bis(2-methoxyetiioxy)quinazolin-4-amine (erlotinib, OSI 774) and 6-acrylamido-N-(3-chloro-4-fluorophenyl)-7-(3- morpholinopropoxy)-quinazolin-4-amine (CI 1033), erbB2 tyrosine kinase inhibitors such as lapatinib, inhibitors of the hepatocyte growth factor family,
  • Ras/Raf signalling inhibitors such as farnesyl transferase inhibitors, for example sorafenib
  • inhibitors of cell signalling through MEK and/or AKT kinases inhibitors of the hepatocyte growth factor family, c-kit inhibitors, abl kinase inhibitors, IGF receptor (insulin-like growth factor) kinase inhibitors; aurora kinase inhibitors (for example AZDl 152,
  • antiangiogenic agents such as those which inhibit the effects of vascular endothelial growth factor, [for example the anti vascular endothelial cell growth factor antibody bevacizumab (AvastinTM) and VEGF receptor tyrosine kinase inhibitors such as 4-
  • vascular damaging agents such as Combretastatin A4 and compounds disclosed in International Patent Applications WO 99/02166, WO 00/40529, WO 00/41669, WO 01/92224, WO 02/04434 and WO 02/08213;
  • antisense therapies for example those which are directed to the targets listed above, such as ISIS 2503, an anti-ras antisense;
  • gene therapy approaches including for example approaches to replace aberrant genes such as aberrant p53 or aberrant BRCAl or BRCA2, GDEPT (gene directed enzyme pro drug therapy) approaches such as those using cytosine deaminase, thymidine kinase or a bacterial nitroreductase enzyme and approaches to increase patient tolerance to chemotherapy or radiotherapy such as multi drug resistance gene therapy;
  • GDEPT gene directed enzyme pro drug therapy
  • immunotherapy approaches including for example ex vivo and in vivo approaches to increase the immunogenicity of patient tumour cells, such as transfection with cytokines such as interleukin 2, interleukin 4 or granulocyte macrophage colony stimulating factor, approaches to decrease T cell anergy, approaches using transfected immune cells such as cytokine transfected dendritic cells, approaches using cytokine transfected tumour cell lines and approaches using anti idiotypic antibodies; and
  • temperatures are given in degrees Celsius ( 0 C); operations were carried out at room or ambient temperature, that is, at a temperature in the range of 18-25 0 C;
  • organic solutions were dried over anhydrous magnesium sulfate; evaporation of solvent was carried out using a rotary evaporator under reduced pressure (600-4000 Pascals; 4.5-30mmHg) with a bath temperature of up to 6O 0 C;
  • chromatography means flash chromatography on silica gel
  • SCX-2 cartridges are Ion Exchange SPE columns where the stationary phase is polymeric propylsulfonic acid. These are used to isolate amines.
  • NMR data when given, NMR data is in the form of delta values for major diagnostic protons, given in parts per million (ppm) relative to tetramethylsilane (TMS) as an internal standard, determined at 400 MHz or 500MHz, in CDCl 3, DMSO-d 6 or DMSO-d6 + ⁇ VAcOH unless otherwise indicated;
  • TMS tetramethylsilane
  • MS Mass spectra
  • HPLC component comprised generally either a Agilent 1100 or Waters Alliance HT (2790 & 2795) equipment and was run on a Phenomenex Gemini Cl 8 5mm, 50 x 2 mm column (or similar) eluting with either acidic eluent (for example, using a gradient between 0 - 95% water / acetonitrile with 5% of a 1% formic acid in 50:50 water: acetonitrile (v/v) mixture; or using an equivalent solvent system with methanol instead of acetonitrile), or basic eluent (for example, using a gradient between 0 - 95% water / acetonitrile with 5% of a 0.1% 880 Ammonia in acetonitrile mixture); and the MS component comprised generally a Waters ZQ mass spectrometer scanning over an appropriate mass range.
  • the following assay was used to measure the effects of the compounds of the present invention as PUc kinase inhibitors.
  • the assay uses Scintillation Proximity Assay (SPA) technology (Antonsson et a!.,
  • Test compounds were prepared as 1OmM stock solutions in dimethylsulfoxide (DMSO) and diluted into water as required to give a range of final assay concentrations. Aliquots (5 ⁇ l) of each compound dilution were dispensed into a well of a 384-well flat bottom white polystyrene plate (Matrix, Catalogue No. 4316).
  • DMSO dimethylsulfoxide
  • a buffer solution comprising 5OmM HEPES pH7.5 buffer, 1OmM manganese chloride (MnCl 2 ), ImM dithiothreitol (D
  • Reactions were stopped by addition of EDTA (HOmM) and the Cdc25C substrate captured via its GST tag to anti-GST antibody (Molecular Probes, Cat No A-5800) coated Protein A PVT SPA beads (Amersham Biosciences, Catalogue No. RPQ0019; 250 ⁇ g/well) in 5OmM HEPES pH7.5 buffer containing 0.05% (w/v) sodium azide and incubated for up to 2 hours, followed by the addition of 20 ⁇ l of 4M caesium chloride (final assay concentration of IM). Plates were then left in the dark overnight before counting on a Packard TopCount NXT.
  • HEPES pH7.5 buffer containing 0.05% (w/v) sodium azide 5OmM HEPES pH7.5 buffer containing 0.05% (w/v) sodium azide
  • Radiolabeled phosphorylated substrate is formed in situ as a result of Plkl mediated phosphorylation.
  • the SPA beads contain a scintillant that can be stimulated to emit light. This stimulation only occurs when a radiolabeled phosphorylated substrate is bound to the surface of the coated SPA bead causing the emission of blue light that can be measured on a scintillation counter. Accordingly, the extent of Plkl mediated Cdc25C phosphorylation was assessed. The raw assay data were then analysed by non-linear regression analysis and Plkl enzyme inhibition for a given test compound is expressed as an IC50 value.
  • H3 on serine 10 Dephosphorylation begins in anaphase and ends at early telophase, thus histone H3 serine 10 phosphorylation acts as an excellent mitotic marker and is used to determine the ability of compounds of the present invention to block cells in mitosis.
  • DMEM phenol red free Dulbecco's Modified Eagles Medium
  • FCS phenol red free Dulbecco's Modified Eagles Medium
  • Test compounds were solubilised in DMSO, diluted to give a range of final assay concentrations, added to cells and incubated for 24h at 37 0 C.
  • Inhibition of PIk leads to an increase in the population of histone H3 SerlO positive cells, indicating inhibition of proliferation is brought about primarily by arrest of cells in the mitotic phase of the cell cycle.
  • the raw assay data were analysed by non-linear regression analysis and used to determine an IC50 value for each compound.
  • Example compounds of this invention were measured to have IC50 values of ⁇ 200nM in the Enzyme assay, and ⁇ 100nM in the Cellular assay.

Abstract

L'invention concerne un composé de formule (I) : ou un sel pharmaceutiquement acceptable de celui-ci. Elle concerne également des procédés de fabrication de ce composé et ses utilisations dans le domaine thérapeutique.
PCT/GB2007/002672 2006-07-17 2007-07-16 Ptéridimones en tant que modulateurs de la kinase de type polo WO2008009909A1 (fr)

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EP2112152A1 (fr) 2008-04-22 2009-10-28 GPC Biotech AG Dihydropteridinones comme inhibiteurs Plk
EP2325185A1 (fr) 2009-10-28 2011-05-25 GPC Biotech AG Inhibiteur de Plk
US9351974B2 (en) 2011-11-10 2016-05-31 OSI Pharmaceuticals, LLC Substituted pteridinones for the treatment of cancer
EP3099171A4 (fr) * 2014-01-31 2017-08-09 Dana-Farber Cancer Institute, Inc. Dérivés de dihydroptéridinone et leurs utilisations
US9975896B2 (en) 2013-07-25 2018-05-22 Dana-Farber Cancer Institute, Inc. Inhibitors of transcription factors and uses thereof
US10150756B2 (en) 2014-01-31 2018-12-11 Dana-Farber Cancer Institute, Inc. Diaminopyrimidine benzenesulfone derivatives and uses thereof
US10308653B2 (en) 2014-08-08 2019-06-04 Dana-Farber Cancer Institute, Inc. Diazepane derivatives and uses thereof
US10702527B2 (en) 2015-06-12 2020-07-07 Dana-Farber Cancer Institute, Inc. Combination therapy of transcription inhibitors and kinase inhibitors
US10793571B2 (en) 2014-01-31 2020-10-06 Dana-Farber Cancer Institute, Inc. Uses of diazepane derivatives
US10881668B2 (en) 2015-09-11 2021-01-05 Dana-Farber Cancer Institute, Inc. Acetamide thienotriazolodiazepines and uses thereof
US10913752B2 (en) 2015-11-25 2021-02-09 Dana-Farber Cancer Institute, Inc. Bivalent bromodomain inhibitors and uses thereof
US11306105B2 (en) 2015-09-11 2022-04-19 Dana-Farber Cancer Institute, Inc. Cyano thienotriazolodiazepines and uses thereof
CN115947728A (zh) * 2021-10-09 2023-04-11 沈阳药科大学 含磺酰基的二氢喋啶酮衍生物及其应用

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WO2003020722A1 (fr) * 2001-09-04 2003-03-13 Boehringer Ingelheim Pharma Gmbh & Co. Kg Nouvelles dihydropteridinones, procedes pour les produire et leur utilisation en tant que medicaments
WO2006018222A1 (fr) * 2004-08-14 2006-02-23 Boehringer Ingelheim International Gmbh Nouveaux hydrates et polymorphes du 4-[[(7r)-8-cyclopentyl-7-ethyl-5,6,7,8-tetrahydro-5-methyl-4-6-oxo-2-piperidinyl]amino]-3-methoxy-n-(1-methyl-4-piperidinyl)-benzamide, son procede de production et son utilisation comme medicament
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Cited By (17)

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WO2009130016A1 (fr) * 2008-04-22 2009-10-29 Gpc Biotech Ag Dihydroptéridinones comme inhibiteurs de plk
EP2112152A1 (fr) 2008-04-22 2009-10-28 GPC Biotech AG Dihydropteridinones comme inhibiteurs Plk
EP2325185A1 (fr) 2009-10-28 2011-05-25 GPC Biotech AG Inhibiteur de Plk
US9351974B2 (en) 2011-11-10 2016-05-31 OSI Pharmaceuticals, LLC Substituted pteridinones for the treatment of cancer
US9975896B2 (en) 2013-07-25 2018-05-22 Dana-Farber Cancer Institute, Inc. Inhibitors of transcription factors and uses thereof
US10793571B2 (en) 2014-01-31 2020-10-06 Dana-Farber Cancer Institute, Inc. Uses of diazepane derivatives
EP3099171A4 (fr) * 2014-01-31 2017-08-09 Dana-Farber Cancer Institute, Inc. Dérivés de dihydroptéridinone et leurs utilisations
US10150756B2 (en) 2014-01-31 2018-12-11 Dana-Farber Cancer Institute, Inc. Diaminopyrimidine benzenesulfone derivatives and uses thereof
US10730860B2 (en) 2014-01-31 2020-08-04 Dana-Farber Cancer Institute, Inc. Diaminopyrimidine benzenesulfone derivatives and uses thereof
US10308653B2 (en) 2014-08-08 2019-06-04 Dana-Farber Cancer Institute, Inc. Diazepane derivatives and uses thereof
US10702527B2 (en) 2015-06-12 2020-07-07 Dana-Farber Cancer Institute, Inc. Combination therapy of transcription inhibitors and kinase inhibitors
US10881668B2 (en) 2015-09-11 2021-01-05 Dana-Farber Cancer Institute, Inc. Acetamide thienotriazolodiazepines and uses thereof
US11306105B2 (en) 2015-09-11 2022-04-19 Dana-Farber Cancer Institute, Inc. Cyano thienotriazolodiazepines and uses thereof
US11406645B2 (en) 2015-09-11 2022-08-09 Dana-Farber Cancer Institute, Inc. Acetamide thienotriazolodiazepines and uses thereof
US10913752B2 (en) 2015-11-25 2021-02-09 Dana-Farber Cancer Institute, Inc. Bivalent bromodomain inhibitors and uses thereof
CN115947728A (zh) * 2021-10-09 2023-04-11 沈阳药科大学 含磺酰基的二氢喋啶酮衍生物及其应用
CN115947728B (zh) * 2021-10-09 2024-01-09 沈阳药科大学 含磺酰基的二氢喋啶酮衍生物及其应用

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