Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D233/00—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings
  • C07D233/04—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member
  • C07D233/28—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
  • C07D233/44—Nitrogen atoms not forming part of a nitro radical
  • C07D233/50—Nitrogen atoms not forming part of a nitro radical with carbocyclic radicals directly attached to said nitrogen atoms
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P27/00—Drugs for disorders of the senses
  • A61P27/02—Ophthalmic agents
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P27/00—Drugs for disorders of the senses
  • A61P27/02—Ophthalmic agents
  • A61P27/06—Antiglaucoma agents or miotics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P9/00—Drugs for disorders of the cardiovascular system
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
  • C07D239/70—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings condensed with carbocyclic rings or ring systems
  • C07D239/72—Quinazolines; Hydrogenated quinazolines
  • C07D239/74—Quinazolines; Hydrogenated quinazolines with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, attached to ring carbon atoms of the hetero ring
  • C07D239/76—N-oxides

Definitions

• the present invention relates to alpha 2 ⁇ adrenergic receptor agonist nitrooxyderivatives and to their use for the treatment of ocular diseases in particular for the treatment of high intraocular pressure and glaucoma.
• Glaucoma occurs in about 2% of all population over the age of 40 and may be asymptomatic for years before progressing to rapid loss of vision.
• Glaucoma is primarily classified as open-angle, closed-angle, or congenital, and further classified as primary and secondary. Glaucoma is treated with a variety of pharmacological and surgical approaches. In cases where glaucoma is associated with ocular hypertension, pharmacological treatment comprises adrenergic agonists
• alpha-adrenergic agonists such as brimonidine and apraclonidine
• control IOP by reducing the production of aqueous humor as well as enhancing uveoscleral outflow.
• Alpha 2 ⁇ adrenergic receptor agonists are also used for the treatment of ocular hypertension and optic neuropathies both in monotherapy and as adjunctive therapy to beta- blockers. They are also used for the prophylactic treatment of acute pressure rises (i.e.
• optical ophthalmic solutions containing alpha 2 ⁇ adrenergic receptor agonists are absorbed systemically and can produce side-effects including systemic hypotension, decreased heart rate, dry mouth, lid retraction, conjunctiva blanching, hyperaemia, burning, uveitis, tachyphilaxis, posterior segment vasoconstriction, topical allergy-like syndrome, increased pupil diameter, depression, anxiety, fatigue, nausea.
• side-effects including systemic hypotension, decreased heart rate, dry mouth, lid retraction, conjunctiva blanching, hyperaemia, burning, uveitis, tachyphilaxis, posterior segment vasoconstriction, topical allergy-like syndrome, increased pupil diameter, depression, anxiety, fatigue, nausea.
• alpha 2 -adrenergic receptor agonists nitrooxyderivatives of formula (I) have a significantly improved overall profile as compared to native compounds with respect to both pharmacological activity and enhanced tolerability.
• Xi has the following meanings: -C(O)-, -C(O)O-,
• Y is a bivalent radical having the following meanings: a)
• Ci-C 10 being optionally substituted with one or more of the substituents selected from the group consisting of: halogen atoms, hydroxy, -ONO2 or T 0 , wherein T 0 is -OC(O) (Ci-Cio alkyl)-0N0 2 or -0(Ci-Ci 0 alkyl) -ONO 2 ;
• T is straight or branched alkyl with from 1 to 10 carbon atoms, preferably CH 3 ;
• n is an integer from 0 to 20, preferably n is from 1 to 10, n 1 is an integer from 1 to 20, preferably n 1 is from 1 to 10; d)
• n 1 is as defined above and n 2 is an integer from 0 to 2;
• X 2 -OCO- or -COO- and R 2 is an hydrogen atom or CH 3 ; e)
• n 1 , n 2 ,R 2 and X 2 are as defined above;
• n 1 and R 2 are as defined above, R 3 is H or -COCH 3 ; with the proviso that when Y is selected from the bivalent radicals mentioned under b) -f) , the -ONO 2 group is linked to a -(CH 2 ) H 1 group; g )
• X 3 is an oxygen atom or a sulphur atom, preferably X 3 is an oxygen atom; n is an integer from 1 to 6, preferably from 1 to 4, R 2 is as defined above; h)
• n 4 is an integer from 0 to 10
• n 5 is an integer from 1 to 10
• R 4 , R 5 , R 6 , R 7 are the same or different, and are H or straight or branched Ci-C 4 alkyl, preferably R 4 , R 5 , R 6 , R 7 are H; wherein the -ONO 2 group is linked to
• Y 2 is an heterocyclic saturated, unsaturated or aromatic 5 or 6 members ring, containing one or more heteroatoms selected from nitrogen, oxygen, sulfur, and is selected from
• C1-C20 alkylene refers to branched or straight chain C 1 -C 20 hydrocarbon, preferably having from 1 to 10 carbon atoms such as methylene, ethylene, propylene, isopropylene, n-butylene, pentylene, n-hexylene and the like.
• Ci-Ci 0 alkyl refers to branched or straight chain alkyl groups comprising one to ten carbon atoms, including methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, t-butyl, pentyl, hexyl, octyl and the like.
• cycloalkylene refers to ring having from 5 to 7 carbon atoms including, but not limited to, cyclopentylene, cyclohexylene optionally substituted with side chains such as straight or branched (Ci-Ci 0 ) - alkyl, preferably CH 3 .
• heterocyclic refers to saturated, unsaturated or aromatic 5 or 6 members ring, containing one or more heteroatoms selected from nitrogen, oxygen, sulphur, such as for example pyridine, pyrazine, pyrimidine, pyrrolidine, morpholine, imidazole and the like.
• nitrogen, oxygen, sulphur such as for example pyridine, pyrazine, pyrimidine, pyrrolidine, morpholine, imidazole and the like.
• Preferred nitrooxyderivatives of formula (I) are:
• Another object of the present invention is pharmaceutical compositions containing at least a compound of the present invention of formula (I) together with non toxic adjuvants and/or carriers usually employed in the pharmaceutical field.
• the preferred route of administration is topical.
• the compounds of the present invention can be administered as solutions, suspensions or emulsions (dispersions) in an ophthalmically acceptable vehicle.
• ophthalmically acceptable vehicle refers to any substance or combination of substances which are non-reactive with the compounds and suitable for administration to patient.
• aqueous vehicles suitable for topical application to the patient's eyes.
• ingredients which may be desirable to use in the ophthalmic compositions of the present invention include antimicrobials, preservatives, co-solvents, surfactants and viscosity building agents.
• the invention also relates to a method for treating glaucoma or ocular hypertension, said method consisting in contacting an effective intraocular pressure reducing amount of a composition with the eye in order to reduce eye pressure and to maintain said pressure on a reduced level.
• the doses of the compounds of the invention can be determined by standard clinical techniques and are in the same range or less than those described for the corresponding underivatized, commercially available compounds as reported in the: Physician's Desk Reference, Medical Economics Company, Inc., Oradell, N.J., 58 th Ed., 2004; The pharmacological basis of therapeutics, Goodman and Gilman, J. G. Hardman, L. e. Limbird, Tenth Ed.
• the treatment may be advantageously carried out in that one drop of the composition, corresponding to about 30 ⁇ l, is administered about several times per day, for example from 1 to 3 times, to the patient's eye.
• the compounds of the present invention can be used with other medicaments known to be useful in the treatment of glaucoma or ocular hypertension, either separately or in combination.
• the compounds of the present invention can be combined with (i) beta-blockers, such as timolol, betaxolol, levobunolol and the like (see U.S. Pat. No. 4,952,581); (ii) carbonic anhydrase inhibitors, such as brinzolamide .
• nitrooxy derivatives of the above reported compounds for example nitrooxy derivatives of beta-blockers such as those described in U.S. Pat. No. 6,242,432.
• the compounds of the present invention can be synthesised as follows.
• A) The compounds of general formula (I) wherein A is the radical (Ia) or (Ib), X 1 is -C(O)-, and Y is as above defined, can be obtained by a process comprising:
• P is H or a amino protecting group such as t-butoxycarbonyl and those described in T. W. Greene “Protective groups in organic synthesis", Harvard University Press, 1980; with a compound of formula (Ia) :
• the reaction of a compound of formula (Ilia) or (HIb) , wherein P is as above defined, with a compound of formula (Ia) wherein Y is as above defined and Act a carboxylic acid activating group used in peptide chemistry as above defined, may be carried out in presence of a inorganic or organic base in an aprotic polar/non-polar solvent such as DMF, THF, acetone or CH 2 Cl 2 at temperatures range between 0°-65°C or in a double phase system H 2 0/Et 2 0 at temperatures range between 20°- 40 0 C; or in the presence of DMAP and a Lewis acid such as Sc(OTf) 3 or Bi(OTf) 3 in solvents such as DMF, CH 2 Cl 2 .
• an aprotic polar/non-polar solvent such as DMF, THF, acetone or CH 2 Cl 2
• Act-H (Ic) wherein Act is as above defined, by conventional esterification reaction with condensing agents as DCC, EDACHCl as well known in the literature.
• the compounds of formula (Ib) as above defined are obtained by reacting the commercially available acids of formula (Id) HaI-Y-COOH (Id) with AgNO 3 in a suitable organic solvent such as acetonitrile or tetrahydrofurane (THF) under nitrogen in the dark at temperatures range between 20° to 80°C; alternatively the reaction with AgNO 3 can be performed under microwave irradiation in solvents such acetonitrile or THF at temperatures in the range between 70-180 °C for short time (1-60 min) .
• a suitable organic solvent such as acetonitrile or tetrahydrofurane (THF)
• P is H or a amino protecting group such as t-butoxycarbonyl and those described in T. W. Greene “Protective groups in organic synthesis", Harvard University Press, 1980; with a compound of formula (Ia') :
• DCC N' - (3-dimethylamino ⁇ ropyl) -N-ethylcarbodiimide hydrochloride
• EDAC N-ethylcarbodiimide hydrochloride
• a catalyst such as N, N- dimethylamino pyridine (DMAP) , or benzotriazol-1-yloxy- tris (dimethylamino) phosphonium hexafluorophosphate (BOP) and a organic base, such as N-methylmorpholine, N, N- diisopropylamine .
• the reaction is carried out in an inert organic solvent dry such as N, N' -dimethylformamide, tetrahydrofuran, benzene, toluene, dioxane, a polyhalogenated aliphatic hydrocarbon at a temperature from -2O 0 C and 40 0 C.
• an inert organic solvent dry such as N, N' -dimethylformamide, tetrahydrofuran, benzene, toluene, dioxane, a polyhalogenated aliphatic hydrocarbon at a temperature from -2O 0 C and 40 0 C.
• the reaction is completed within a time range from 30 minutes to 36 hours.
• a suitable organic solvent such as acetonitrile or tetrahydrofurane (THF) under nitrogen in the dark at temperatures range between 20° to 80 0 C
• solvents such as acetonitrile or THF
• P is H or a amino protecting group such as t-butoxycarbonyl and those described in T. W. Greene “Protective groups in organic synthesis", Harvard University Press, 1980; with a compound of formula (Ia'') :
• a suitable organic solvent such as acetonitrile or tetrahydrofuran (THF)
• THF tetrahydrofuran
• HO-Y-ONO 2 (If) wherein Y is as above defined, in presence of an inorganic or organic base in an aprotic polar/non-polar solvent such as DMF, THF or CH2CI2 at temperatures range between 0° to 65 0 C or in a double phase system H 2 O/Et 2 ⁇ at temperatures range between 20° to 40 0 C, lB.b)
• the compounds of formula (If) are obtained by reacting the commercially available compounds of formula HO-Y-HaI (If) wherein Y and Hal are as above defined, with AgN ⁇ 3 in a suitable organic solvent such as acetonitrile or tetrahydrofurane (THF) under nitrogen in the dark at temperatures range between 20°-80°C; alternatively the reaction with AgNU3 can be performed under microwave irradiation in solvents such acetonitrile or THF at temperatures in the range between about 100-180 0 C for time range about 1-60 min .
• aprotic polar/non-polar solvent such as DMF, THF or CH 2 Cl 2
• Y is as above defined
• HaI-C(O)-O-Y-ONO 2 (Ia. ii) wherein Hal is an halogen atom, preferably is Cl, and Y is as above defined, in presence of a inorganic or organic base/DMAP in an aprotic polar/non-polar solvent such as DMF, THF or CH 2 Cl 2 at temperatures range between 0° to 65 0 C or in a double phase system H 2 0/Et 2 0 at temperatures range between 20° to 40°C; or in the presence of DMAP and a Lewis acid such as Sc(OTf) 3 or Bi(OTf) 3 in solvents such as DMF, CH 2 Cl 2 ; and then removing the protective group of the obtained compounds as described in IA. a); and optionally converting the resulting compounds of formula (I) into a pharmaceutically acceptable salt.
• aprotic polar/non-polar solvent such as DMF, THF or CH 2 Cl 2
• ICb The compounds of formula (Ia. ii) as above defined, are obtained by reacting a compounds of formula (If) HO-Y-ONO 2 (If) and phosgene and its derivatives such as triphosgene in the presence of a inorganic or organic base in an aprotic polar/non-polar solvent such as DMF, THF or CH 2 Cl 2 at temperatures range between 0° to 65 0 C, ICc)
• aprotic polar/non-polar solvent such as DMF, THF or CH 2 Cl 2
• Y is as above defined, can be obtained by a process comprising: ID) reacting a compound of formula (Ilia) or (HIb)
• P is H or a amino protecting group such as t-butoxycarbonyl and those described in T. W. Greene "Protective groups in organic synthesis", Harvard University Press, 1980; with compounds of formula (la.iii)
• HaI-W 4 -OC(O)O-Y-ONO 2 (la.iii) wherein Hal is an halogen atom and W 4 is -CH 2 - or -CH(CH 3 )-, in presence of a inorganic or organic base in an aprotic polar/non-polar solvent such as DMF, THF or CH 2 Cl 2 at temperatures range between 0° to 65 0 C or in a double phase system H 2 CVEt 2 O at temperatures range between 20° to 40 0 C; and then removing the protective group of the obtained compounds as described in IA. a) . ID. a) The compounds of formula (la.iii) are obtained by reacting the commercially available haloalkylhalocarbonate of formula (Ig)
• HIa' (HIb') wherein Q is selected from a chlorine atom, a bromine atom, a iodine atom, mesyl, tosyl with a nitrate source such as silver nitrate, lithium nitrate, sodium nitrate, potassium nitrate, magnesium nitrate, calcium nitrate, iron nitrate, zinc nitrate or tetraalkylammonium nitrate (wherein alkyl is Ci-Cio alkyl) in a suitable organic solvent such as acetonitrile, tetrahydrofurane, methyl ethyl ketone, ethyl acetate, DMF, the reaction is carried out, in the dark, at a temperature ranges from room temperature to the boiling point temperature of the solvent.
• a nitrate source such as silver nitrate, lithium nitrate, sodium nitrate, potassium nitrate, magnesium nitrate, calcium
• the preferred nitrate source is silver nitrate; and then lE.b) removing the protective group with the methods known in the art; and optionally converting the resulting compound of general formula (I) into a pharmaceutically acceptable salt.
• lE.c) The compounds of formula (HIa') or (UIb') as above defined are obtained by reacting compounds of formula (HIa) and (HIb) wherein P is as above defined, with compounds of formula (Ih) ACt-C(O)-Y-HaI (Ih) or compounds of formula (11)
• ACt-C(O)-Y-HaI (Ih) as above defined, are obtained by reacting commercially available (Ic) Act-H (Ic) with the commercially available compounds of formula (Id)
• the compounds of general formula (I) wherein A is the radical (Ia) or (Ib), X 1 is -C(O) or - C(O)O-, and Y is as above defined can be obtained by a process comprising: IF. a) reacting a compound of formula (HIa'') or (HIb'')
• P is H or a amino protecting group such as t-butoxycarbonyl and those described in T. W. Greene “Protective groups in organic synthesis", Harvard University Press, 1980, with triflic anhydride/tetraalkylammonium nitrate salt in an aprotic polar/non-polar solvent such as DMF, THF or CH 2 CI 2 at temperatures range between -60° to 65 0 C; lF.b) removing the protective group with the methods known in the art; and optionally converting the compound of formula (I) into a pharmaceutically acceptable salt.
• lF.c The compounds of formula (HIa'') or (HIb'') are obtained by reacting the compounds of formula (HIa) or (HIb) wherein P is as above defined, with compounds of formula (Im)
• ACt-C(O)-O-Y-OH (In) wherein Act and Y are as above defined, in presence of a inorganic or organic base/DMAP in an aprotic polar/non- polar solvent such as DMF, THF or CH 2 Cl 2 at temperatures range between 0° to 65 0 C or in a double phase system H 2 0/Et 2 0 at temperatures range between 20° to 40 0 C; or in the presence of DMAP and a Lewis acid such as Sc (OTf) 3 or Bi(OTf) 3 in solvents such as DMF, CH 2 Cl 2 ; lF.d)
• the compounds of formula (Im) ACt-C(O)-Y-OH (Im) are obtained by reacting commercially available (Ic) Act-H (Ic) with the commercially available compounds of formula (lo)
• ACt-C(O)-O-Y-OH (In) are obtained by reacting compounds of formula (Ie) ACt-C (O) -HaI ( Ie ) which are commercially available or are obtained as described in lB.c), with a compounds of formula (Ij)
• an aprotic polar/non-polar solvent such as DMF, THF or CH 2 Cl 2
• Reagents and conditions a) AgNO 3 , CH 3 CN, r.t., 24 h; b) Triphosgene, Et 3 N, benzene, 0 - 20 0 C, 12 h; c) Et 3 N, DMF, 40 h.
• Reagents and conditions a) ZnNO 3 , DCC, CH 3 CN, r . t . ; b) Triphosgene, Et 3 N, benzene, 0 - 20°C, 12 h; c) Et 3 N, DMF, 64 h.
• Alcohol A-Il (0.7g) was added to a cold solution of triphosgene (0.77 g) in benzene (5 mL) . The mixture was stirred at 0 0 C for more than 20 min. The solution of Et 3 N (0.53 g) in benzene (5 mL) was added dropwise to the reaction mixture. The mixture was warmed to room temperature and stirred overnight. The excess phosgene was removed by bubbling a stream of dry nitrogen through. Then the reaction mixture was evaporated and the residues was dissolved in Et2O, and filtered to remove the salt. The collected solid was washed with Et 2 O. The combined filtrate was evaporated under vacuum to give Compound A-12 as a light yellow oil (0.5 g) . The crude product was used in the next step without further purification.

Landscapes

• Organic Chemistry (AREA)
• Chemical & Material Sciences (AREA)
• Health & Medical Sciences (AREA)
• Medicinal Chemistry (AREA)
• General Chemical & Material Sciences (AREA)
• Veterinary Medicine (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Life Sciences & Earth Sciences (AREA)
• Pharmacology & Pharmacy (AREA)
• Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
• Public Health (AREA)
• General Health & Medical Sciences (AREA)
• Animal Behavior & Ethology (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Engineering & Computer Science (AREA)
• Ophthalmology & Optometry (AREA)
• Heart & Thoracic Surgery (AREA)
• Cardiology (AREA)
• Pain & Pain Management (AREA)
• Rheumatology (AREA)
• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
• Plural Heterocyclic Compounds (AREA)
• Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Priority Applications (4)

Applications Claiming Priority (2)

Publications (1)

Family

ID=37810330

Family Applications (1)

Country Status (5)

Cited By (5)

Families Citing this family (2)

Citations (4)

• 2007
  • 2007-02-02 US US12/162,529 patent/US7799784B2/en not_active Expired - Fee Related
  • 2007-02-02 EP EP07726294A patent/EP1981852A1/en not_active Withdrawn
  • 2007-02-02 CA CA002641501A patent/CA2641501A1/en not_active Abandoned
  • 2007-02-02 JP JP2008552815A patent/JP2009525970A/ja not_active Withdrawn
  • 2007-02-02 WO PCT/EP2007/051017 patent/WO2007090793A1/en not_active Ceased

Patent Citations (4)

Also Published As

Similar Documents

Legal Events