WO2007075988A3 - Detection non invasive de virus frappant les poissons au moyen d'une pcr en temps reel - Google Patents
Detection non invasive de virus frappant les poissons au moyen d'une pcr en temps reel Download PDFInfo
- Publication number
- WO2007075988A3 WO2007075988A3 PCT/US2006/048976 US2006048976W WO2007075988A3 WO 2007075988 A3 WO2007075988 A3 WO 2007075988A3 US 2006048976 W US2006048976 W US 2006048976W WO 2007075988 A3 WO2007075988 A3 WO 2007075988A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- ihnv
- gene
- real
- samples
- spleen
- Prior art date
Links
- 241000700605 Viruses Species 0.000 title abstract 6
- 241000251468 Actinopterygii Species 0.000 title abstract 4
- 238000001514 detection method Methods 0.000 title abstract 4
- 238000003753 real-time PCR Methods 0.000 title abstract 4
- 101150082239 G gene Proteins 0.000 abstract 4
- 238000003556 assay Methods 0.000 abstract 4
- 210000000952 spleen Anatomy 0.000 abstract 4
- 210000000577 adipose tissue Anatomy 0.000 abstract 3
- 230000014509 gene expression Effects 0.000 abstract 3
- 210000003734 kidney Anatomy 0.000 abstract 3
- 210000004185 liver Anatomy 0.000 abstract 3
- 210000000006 pectoral fin Anatomy 0.000 abstract 3
- 210000001519 tissue Anatomy 0.000 abstract 3
- 241000277331 Salmonidae Species 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 238000011002 quantification Methods 0.000 abstract 2
- 238000005070 sampling Methods 0.000 abstract 2
- 102000007469 Actins Human genes 0.000 abstract 1
- 108010085238 Actins Proteins 0.000 abstract 1
- 108091093088 Amplicon Proteins 0.000 abstract 1
- 201000009030 Carcinoma Diseases 0.000 abstract 1
- 102000012750 Membrane Glycoproteins Human genes 0.000 abstract 1
- 108010090054 Membrane Glycoproteins Proteins 0.000 abstract 1
- 206010028980 Neoplasm Diseases 0.000 abstract 1
- 238000009360 aquaculture Methods 0.000 abstract 1
- 244000144974 aquaculture Species 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 239000002299 complementary DNA Substances 0.000 abstract 1
- 238000010494 dissociation reaction Methods 0.000 abstract 1
- 230000005593 dissociations Effects 0.000 abstract 1
- 208000010932 epithelial neoplasm Diseases 0.000 abstract 1
- 208000015181 infectious disease Diseases 0.000 abstract 1
- 230000002458 infectious effect Effects 0.000 abstract 1
- 238000002844 melting Methods 0.000 abstract 1
- 230000008018 melting Effects 0.000 abstract 1
- 230000017074 necrotic cell death Effects 0.000 abstract 1
- 230000010076 replication Effects 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention concerne un essai en temps réel comprenant l'échantillonnage de tissus non invasifs, développé pour la détection et la quantification des virus frappant les poissons. Dans la démonstration du principe, des données concernant la détection et la quantification du virus de la nécrose hypodermique infectieuse chez la truite sont présentées. Les amorces sont créées pour un nucléocapside (N) d'IHNV, et des gènes (G) de la glycoprotéine de surface, et la ß-actine de la truite et le facteur 1a- d'élongation (EF- Ia) sont utilisés comme moyen de contrôle interne pour l'essai. Les conditions de réaction pour la PCR en temps réel sont optimisées au moyen d'un ADNc dérivé des cellules de l'épithéliome papulosum des cyprinidés (EPC). Grâce aux amorces des gènes N et G, l'IHNV est détecté dans des échantillons du foie, du rein, de la rate, des tissus adipeux et des nageoires pectorales provenant d'échantillons sauvages et élevés en laboratoire. Les courbes de dissociation présentant un pic de fusion simple à une température souhaitée (85 °C pour le gène N et 86,5 °C pour le gène G) confirment la spécificité des amplicons desdits gènes N et G. Les niveaux d'expression des gènes G et N de l'IHNV dans différents tissus d'échantillons élevés en laboratoire sont dans l'ordre : la rate, le foie, le rein, les tissus adipeux et la nageoire pectorale, alors que pour les échantillons prélevés dans le milieu, l'ordre de l'expression du gène est : le foie, le rein, la nageoire pectorale, les tissus adipeux et la rate. Les expressions des gènes G et N détectées dans la rate sont dramatiquement inférieures dans les échantillons prélevés dans le milieu à celles des échantillons prélevés en laboratoire, ce qui indique une différence potentielle dans la réplication de l'IHNV en laboratoire par rapport aux conditions dans le milieu. Sur la base de la capacité à détecter le virus dans les nageoires pectorales, un procédé de détection non invasif est utilisé pour l'IHNV ainsi que pour d'autres virus frappant les poissons. L'échantillonnage des tissus non invasif couplé à un essai par PCR est tout à fait valable pour un criblage du virus à grande échelle chez les poissons, dans des installations aquacoles ainsi que dans des études épidémiologiques.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/158,096 US20090181363A1 (en) | 2005-12-21 | 2006-12-21 | Non-invasive detection of fish viruses by real-time pcr |
| CA002638900A CA2638900A1 (fr) | 2005-12-21 | 2006-12-21 | Detection non invasive de virus frappant les poissons au moyen d'une pcr en temps reel |
| GB0813134A GB2447593A (en) | 2005-12-21 | 2008-07-18 | Non-invasive detection of fish viruses by real-time PCR |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US75293005P | 2005-12-21 | 2005-12-21 | |
| US60/752,930 | 2005-12-21 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2007075988A2 WO2007075988A2 (fr) | 2007-07-05 |
| WO2007075988A3 true WO2007075988A3 (fr) | 2008-06-26 |
Family
ID=38218655
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2006/048976 WO2007075988A2 (fr) | 2005-12-21 | 2006-12-21 | Detection non invasive de virus frappant les poissons au moyen d'une pcr en temps reel |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20090181363A1 (fr) |
| CA (1) | CA2638900A1 (fr) |
| GB (1) | GB2447593A (fr) |
| WO (1) | WO2007075988A2 (fr) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8968721B2 (en) | 2005-12-28 | 2015-03-03 | Advanced Bionutrition Corporation | Delivery vehicle for probiotic bacteria comprising a dry matrix of polysaccharides, saccharides and polyols in a glass form and methods of making same |
| ES2470340T3 (es) | 2005-12-28 | 2014-06-23 | Advanced Bionutrition Corporation | Vehículo de administración para bacterias probi�ticas que comprende una matriz seca de polisac�ridos, sac�ridos y polioles en forma vítrea |
| US8460726B2 (en) | 2006-12-18 | 2013-06-11 | Advanced Bionutrition Corporation | Dry food product containing live probiotic |
| US8221767B2 (en) | 2006-12-20 | 2012-07-17 | Advanced Bionutrition Corporation | Antigenicity of infectious pancreatic necrosis virus VP2 sub-viral particles expressed in yeast |
| US8778384B2 (en) | 2008-03-24 | 2014-07-15 | Advanced Bionutrition Corporation | Compositions and methods for encapsulating vaccines for the oral vaccination and boostering of fish and other animals |
| US7998502B2 (en) | 2008-03-24 | 2011-08-16 | Advanced Bionutrition Corp. | Encapsulated vaccines for the oral vaccination and boostering of fish and other animals |
| EP2410996B1 (fr) | 2009-03-27 | 2017-08-02 | Advanced Bionutrition Corp. | Vaccins microparticulaires utilisables pour procéder à une vaccination et à des rappels par voie orale ou nasale chez les animaux, dont les poissons |
| NZ597053A (en) | 2009-05-26 | 2014-02-28 | Advanced Bionutrition Corp | Stable dry powder composition comprising biologically active microorganisms and/or bioactive materials and methods of making |
| EP2443260A4 (fr) * | 2009-06-19 | 2012-11-28 | Commw Scient Ind Res Org | Analyse virale |
| US9504750B2 (en) | 2010-01-28 | 2016-11-29 | Advanced Bionutrition Corporation | Stabilizing composition for biological materials |
| WO2011094469A2 (fr) | 2010-01-28 | 2011-08-04 | Advanced Bionutrition Corporation | Composition vitreuse sèche comprenant un matériau bioactif |
| LT2603100T (lt) | 2010-08-13 | 2018-07-25 | Advanced Bionutrition Corp. | Stabilizuojanti kompozicija, skirta biologinių medžiagų sausam saugojimui |
| RU2508547C2 (ru) * | 2012-03-05 | 2014-02-27 | Государственное научное учреждение Всероссийский научно-исследовательский институт экспериментальной ветеринарии им. Я.Р. Коваленко (ВИЭВ) | Способ диагностики вируса инфекционного некроза поджелудочной железы лососевых методом полимеразной цепной реакции |
| CA2994112C (fr) | 2015-07-29 | 2023-08-08 | Advanced Bionutrition Corp. | Compositions probiotiques seches stables pour des utilisations dietetiques |
| KR101677012B1 (ko) * | 2016-04-27 | 2016-11-17 | 대한민국 | 고병원성 바이러스성 출혈성 패혈증 바이러스 검출용 바이오마커 및 진단방법 |
| CN109439767A (zh) * | 2018-11-21 | 2019-03-08 | 广东省食品检验所(广东省酒类检测中心) | 一种同时鉴别大西洋鲑鱼和虹鳟鱼的双重实时荧光pcr引物组、试剂盒及方法 |
| CN113215112B (zh) * | 2021-05-25 | 2021-11-30 | 中国水产科学研究院长江水产研究所 | 分离的致病性黄颡鱼杯状病毒及其特异性序列及应用 |
| CN113403427B (zh) * | 2021-07-16 | 2023-04-14 | 中国水产科学研究院黄海水产研究所 | 一种反转录多重套式pcr检测引物 |
| CN114592091B (zh) * | 2022-03-18 | 2023-11-03 | 杭州奥盛仪器有限公司 | 一种用于加州鲈鱼弹状病毒检测的试剂盒及其检测方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1559328A1 (fr) * | 2001-03-23 | 2005-08-03 | Advanced Bionutrition Corporation | Aliments de bétail comprenant des fungi pour l'aquaculture et l'élevage du bétail |
| WO2003017780A1 (fr) * | 2001-08-27 | 2003-03-06 | Advanced Bionutrition Corporation | Apport de produits de lutte contre les maladies en aquaculture et agriculture au moyen d'aliments nutritionnels contenant des proteines bioactives produites par des virus |
| US7550647B2 (en) * | 2001-09-14 | 2009-06-23 | Advanced Bionutrition | Transfected shrimp as production systems for therapeutic proteins |
| US7396548B2 (en) * | 2002-03-19 | 2008-07-08 | Advanced Bionutrition Corporation | Microalgal feeds containing arachidonic acid and their production and use |
| EP1497643A4 (fr) * | 2002-04-09 | 2008-07-30 | F C Thomas Allnut | Systemes d'aquacultures clos destines a produire des proteines recombinees purifiees |
| WO2003089579A2 (fr) * | 2002-04-15 | 2003-10-30 | Advanced Bionutrition Corporation | Incorporation de bacteries anaerobiques dans une formulation alimentaire |
| AU2003251604A1 (en) * | 2002-06-26 | 2004-01-19 | F. C. Thomas Allnutt | Viruses and virus-like particles for multiple antigen and target display |
| AU2003279819A1 (en) * | 2002-10-24 | 2004-05-13 | Advanced Bionutrition Corporation | Shrimp and the production thereof |
| US20070082008A1 (en) * | 2003-03-07 | 2007-04-12 | Advanced Bionutrition Corporation | Feed formulation for terrestrial and aquatic animals |
| US20060265766A1 (en) * | 2003-03-19 | 2006-11-23 | Advanced Bionutrition Corporation | Fish and the production thereof |
| WO2004112767A1 (fr) * | 2003-06-19 | 2004-12-29 | Advanced Bionutriton Corporation | Absorption amelioree de nutriants liposolubles |
| AU2005213981A1 (en) * | 2004-02-06 | 2005-09-01 | Advanced Bionutrition Corporation | RNA-mediated interference to control disease in terrestrial and aquaculture animals |
| JP4829223B2 (ja) * | 2004-05-25 | 2011-12-07 | グーグル インコーポレイテッド | 電子メッセージソース評判情報システム |
| JP3979405B2 (ja) * | 2004-07-13 | 2007-09-19 | セイコーエプソン株式会社 | 電気光学装置、実装構造体及び電子機器 |
| US20080044081A1 (en) * | 2006-08-21 | 2008-02-21 | Adam Lieb | Color indexing for images |
-
2006
- 2006-12-21 CA CA002638900A patent/CA2638900A1/fr not_active Abandoned
- 2006-12-21 US US12/158,096 patent/US20090181363A1/en not_active Abandoned
- 2006-12-21 WO PCT/US2006/048976 patent/WO2007075988A2/fr active Application Filing
-
2008
- 2008-07-18 GB GB0813134A patent/GB2447593A/en not_active Withdrawn
Non-Patent Citations (2)
| Title |
|---|
| DEVOLD M. ET AL., DISEASES OF AQUATIC ORGANISMS, vol. 40, 2000, pages 9 - 18, XP000864789 * |
| PURCELL M.K. ET AL., FISH & SHELLFISH IMMUNOLOGY, vol. 17, 2004, pages 447 - 462 * |
Also Published As
| Publication number | Publication date |
|---|---|
| GB2447593A (en) | 2008-09-17 |
| WO2007075988A2 (fr) | 2007-07-05 |
| CA2638900A1 (fr) | 2007-07-05 |
| US20090181363A1 (en) | 2009-07-16 |
| GB0813134D0 (en) | 2008-08-27 |
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