WO2007024125A1 - Fibrous 3-dimensional scaffold via electrospinning for tissue regeneration and method for preparing the same - Google Patents
Fibrous 3-dimensional scaffold via electrospinning for tissue regeneration and method for preparing the same Download PDFInfo
- Publication number
- WO2007024125A1 WO2007024125A1 PCT/KR2006/003390 KR2006003390W WO2007024125A1 WO 2007024125 A1 WO2007024125 A1 WO 2007024125A1 KR 2006003390 W KR2006003390 W KR 2006003390W WO 2007024125 A1 WO2007024125 A1 WO 2007024125A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- tissue regeneration
- preparing
- fibrous porous
- dimensional scaffold
- polymer
- Prior art date
Links
- 230000017423 tissue regeneration Effects 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 33
- 238000001523 electrospinning Methods 0.000 title claims abstract description 21
- 230000021164 cell adhesion Effects 0.000 claims abstract description 10
- 239000000463 material Substances 0.000 claims abstract description 7
- 230000008929 regeneration Effects 0.000 claims abstract description 7
- 238000011069 regeneration method Methods 0.000 claims abstract description 7
- 230000010261 cell growth Effects 0.000 claims abstract description 5
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 claims description 48
- 229920000642 polymer Polymers 0.000 claims description 37
- 239000000835 fiber Substances 0.000 claims description 35
- 238000009987 spinning Methods 0.000 claims description 34
- 229920001432 poly(L-lactide) Polymers 0.000 claims description 21
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 claims description 17
- 239000003960 organic solvent Substances 0.000 claims description 17
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 14
- -1 poly(caprolactone) Polymers 0.000 claims description 10
- 210000004027 cell Anatomy 0.000 claims description 9
- 229920001059 synthetic polymer Polymers 0.000 claims description 8
- 150000001875 compounds Chemical class 0.000 claims description 7
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims description 6
- 229920005615 natural polymer Polymers 0.000 claims description 6
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 4
- 229920000954 Polyglycolide Polymers 0.000 claims description 4
- 229920003232 aliphatic polyester Polymers 0.000 claims description 4
- 238000009835 boiling Methods 0.000 claims description 4
- 238000002513 implantation Methods 0.000 claims description 4
- 239000004633 polyglycolic acid Substances 0.000 claims description 4
- 239000004626 polylactic acid Substances 0.000 claims description 4
- 102000008186 Collagen Human genes 0.000 claims description 3
- 108010035532 Collagen Proteins 0.000 claims description 3
- 229920001436 collagen Polymers 0.000 claims description 3
- 229960005188 collagen Drugs 0.000 claims description 3
- 210000002889 endothelial cell Anatomy 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 210000004409 osteocyte Anatomy 0.000 claims description 3
- 210000004927 skin cell Anatomy 0.000 claims description 3
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims description 2
- UJPMYEOUBPIPHQ-UHFFFAOYSA-N 1,1,1-trifluoroethane Chemical compound CC(F)(F)F UJPMYEOUBPIPHQ-UHFFFAOYSA-N 0.000 claims description 2
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 claims description 2
- 229920002101 Chitin Polymers 0.000 claims description 2
- 229920001661 Chitosan Polymers 0.000 claims description 2
- 108010010803 Gelatin Proteins 0.000 claims description 2
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 claims description 2
- 229920000615 alginic acid Polymers 0.000 claims description 2
- 235000010443 alginic acid Nutrition 0.000 claims description 2
- 229960001126 alginic acid Drugs 0.000 claims description 2
- 239000000783 alginic acid Substances 0.000 claims description 2
- 150000004781 alginic acids Chemical class 0.000 claims description 2
- 210000002449 bone cell Anatomy 0.000 claims description 2
- 210000003321 cartilage cell Anatomy 0.000 claims description 2
- 230000002950 deficient Effects 0.000 claims description 2
- 150000002009 diols Chemical class 0.000 claims description 2
- 229920000159 gelatin Polymers 0.000 claims description 2
- 239000008273 gelatin Substances 0.000 claims description 2
- 229940014259 gelatin Drugs 0.000 claims description 2
- 235000019322 gelatine Nutrition 0.000 claims description 2
- 235000011852 gelatine desserts Nutrition 0.000 claims description 2
- 229920002674 hyaluronan Polymers 0.000 claims description 2
- 229960003160 hyaluronic acid Drugs 0.000 claims description 2
- 238000000465 moulding Methods 0.000 claims description 2
- 229920001610 polycaprolactone Polymers 0.000 claims description 2
- 229920006149 polyester-amide block copolymer Polymers 0.000 claims description 2
- 210000000130 stem cell Anatomy 0.000 claims description 2
- ZOCHHNOQQHDWHG-UHFFFAOYSA-N hexan-3-ol Chemical compound CCCC(O)CC ZOCHHNOQQHDWHG-UHFFFAOYSA-N 0.000 claims 1
- 239000002121 nanofiber Substances 0.000 abstract description 9
- 229920001410 Microfiber Polymers 0.000 abstract description 8
- 239000003658 microfiber Substances 0.000 abstract description 8
- 230000003592 biomimetic effect Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 40
- 239000002904 solvent Substances 0.000 description 12
- 230000005684 electric field Effects 0.000 description 10
- 239000004621 biodegradable polymer Substances 0.000 description 6
- 239000011148 porous material Substances 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 6
- 230000001413 cellular effect Effects 0.000 description 5
- 210000000963 osteoblast Anatomy 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 229920002988 biodegradable polymer Polymers 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 239000011159 matrix material Substances 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 3
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 3
- 239000012620 biological material Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000000839 emulsion Substances 0.000 description 3
- 210000002744 extracellular matrix Anatomy 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 238000005191 phase separation Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 229960000587 glutaral Drugs 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 238000002386 leaching Methods 0.000 description 2
- 239000002062 molecular scaffold Substances 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
- 229920005594 polymer fiber Polymers 0.000 description 2
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 239000013557 residual solvent Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000000859 sublimation Methods 0.000 description 1
- 230000008022 sublimation Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
- A61L27/44—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
- A61L27/48—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix with macromolecular fillers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0015—Electro-spinning characterised by the initial state of the material
- D01D5/003—Electro-spinning characterised by the initial state of the material the material being a polymer solution or dispersion
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
Definitions
- the present invention relates to a fibrous 3-dimensional porous scaffold via elec- trospinning for tissue regeneration and a method for preparing the same.
- Tissue regeneration is induced by supplying cells or drug loaded matrix when tissues or organs lose their functions or are damaged.
- a scaffold for tissue regeneration has to be physically stable in the implanted site, has to be physiologically active to control regeneration efficacy, has to be easily degraded in vivo after generating new tissues and must not produce degradation products with toxicity.
- the conventional scaffolds for tissue regeneration have been produced by using polymers having a certain strength and form, for example sponge type or fibrous matrix or gel type cell culture scaffold has been used.
- the conventional fibrous matrix scaffold has open cellular pores and the pore size is enough size that cells are easily adhered and proliferated.
- the fibrous matrix scaffold is not commonly used today as its disadvantages have been confirmed as follows; a scaffold composed of natural polymer has so poor strength in water phase that it might be destroyed or contracted to lose its original form, and even a synthetic polymer scaffold cannot secure a room with its fibrous structure alone, so that it ends in the membrane shaped 2-dimensional structure rather than 3-dimensional structure.
- the 3-dimensional structure is very important for tissue regeneration and activity. So, such scaffolds having only 2-dimensional structure are limited in applications since it is very difficult with these scaffolds to envelop a medicine and regulate its release or to employ a natural polymer with high physiological activity.
- the preparing method of a sponge type scaffold has been generally accepted for the preparation of conventional scaffolds for tissue generation, for example, particle leaching, emulsion freeze-drying, high pressure gas expansion and phase separation, etc.
- the particle leaching technique is that particles which are insoluble in biodegradable polymer with organic solvent such as salt are mixed with a casting, a solvent is evapotated and then the salt particles are eliminated by elution in water.
- a porous structure with cellular pores in different sizes and various porosities can be obtained by regulating the size of the salt particle and the mixing ratio.
- the remaining salts or rough surfaces cause cell damage (Mikos et al., Biomaterials, 14: 323-330, 1993; Mikos et al., Polymer, 35: 1068-1077, 1994).
- Emulsion freeze-drying is the method that the emulsion of a polymer with organic solvent and water is freeze-dried to eliminate the residual solvents.
- high pressure gas expansion method does not use any organic solvent.
- a bio-degradable polymer is introduced into a mold and pressure is given thereto to prepare pellet.
- high pressure carbon dioxide is injected into the biodegradable polymer at a proper temperature and then the pressure is reduced to release carbon dioxide in the mold to form cellular pores.
- the above methods are also limited in producing open cellular pores (Wang et al., Polymer, 36: 837-842, 1995; Mooney et al., Biomaterials, 17: 1417-1422, 1996).
- a polymer scaffold prepared by using electrospinning has been evaluated, but re- sultingly confirmed that it ends up in 2-dimensional membrane structure, which means it is very difficult to use this scaffold as a 3-dimensional structured implantation material with successful cell adhesion (Yang et al., J. Biomater. Sci. Polymer Edn., 5:1483-1479, 2004; Yang et al., Biomaterials, 26: 2603-2610, 2005).
- An extracellular matrix in vivo has a network-structure composed of basic materials such as glycosaminoglycan and collagen nanofiber, in which cells are adhered and proliferated to form tissues.
- the present invention provides a fibrous porous
- 3-dimensional scaffold for tissue regeneration comprising a polymer fiber having a 3-dimensional network structure using electrospinning.
- the present invention also provides a method for preparing the fibrous porous
- the present invention provides a fibrous porous 3-dimensional scaffold for tissue regeneration having a 3-dimensional network structure comprising a polymer fiber having the size of between nanofiber and microfiber.
- Figs. 2, 3 and 4 illustrate examples of the fibrous porous scaffolds of the invention which are 3 — 12 D in diameter, which is the size of between nanofiber (1 — 500 nm) and microfiber (30 — 50 D).
- the scaffold of the invention has as small fiber diameter as possible to provide large surface area for successful cell adhesion and proliferation and at the same time a regular form and strength to enhance 3-dimensional tissue regeneration capacity.
- the fibrous porous scaffold of the present invention contains a bio-degradable polymer composed of one or more natural polymers selected from a group consisting of chitosan, chitin, alginic acid, collagen, gelatin and hyaluronic acid and a biodegradable polymer composed of a representative bio-degradable aliphatic polyester selected from a group consisting of polylactic acid (PLA), polyglycolic acid (PGA), poly(D,L-lactide-co-glycolide) (PLGA), poly(caprolactone), diol/diacid aliphatic polyester and polyester-amide/polyester-urethane and one or more synthetic polymers selected from a group consisting of poly(valerolactone), poly(hydroxyl butyrate) and poly(hydroxyl valerate).
- PLA polylactic acid
- PGA polyglycolic acid
- PLGA poly(D,L-lactide-co-glycolide)
- PLGA poly(caprolact
- the synthetic polymer is preferably polylactic acid (PLA) having the molecular weight of 100,000 — 350,000 kD, but not always limited thereto.
- the synthetic polymer is more preferably poly L-lactic acid (PLLA).
- Either a natural polymer or a synthetic polymer can be used alone or both of them can be used at the same time as a mixture.
- the fibrous porous scaffold of the present invention has the size of between nanofiber and microfiber, preferably 1 — 15 D in diameter, and a regular form and strength under a proper pressure to help 3-dimensional tissue regeneration and at the same time to provide a large surface area for cell adhesion, so that it can be effectively used for adhesion and proliferation of such cells as endothelial cells, skin cells and osteocytes.
- the scaffold of the invention can be simply prepared by using electrospinning without wasting of polymers or drugs, so it can be more efficient than any other method.
- the fibrous porous scaffold of the present invention can include not only a polymer but also a synthetic low molecular compound.
- the present invention also provides a method for preparing the porous fibrous scaffold with polymer.
- the present invention provides a method for preparing the fibrous porous scaffold comprising the following steps:
- step (i) to prepare the spinning solution, a natural polymer or a synthetic polymer is dissolved in an organic solvent singly or together and a drug is additionally dissolved therein.
- step (i) poly L-lactic acid (PLLA) was dissolved in the organic solvent.
- Any volatile organic solvent having a low boiling point can be used as an organic solvent for the invention to dissolve the synthetic polymer above and particularly chloroform, dichloromethane, dimethylformamide, dioxane, acetone, tetrahydrofurane, trifluoroethane and 1,1,1,3,3,3,-hexafluoroisopropylpropanol are preferred and dichloromethane is more preferred but not always limited thereto.
- the polymer solution drips on a collector by electrospinning and at this time the solvent is entirely volatilized. Because of electrostatic repulsive power, there is no direct contact between fiber and fiber, indicating that fibers are integrated separately. What is most important in this process is that all the solvent has to be volatilized before the drip of the polymer solution on the collector, for which the boiling point of the solvent has to be very low and viscosity of the solvent has to be properly adjusted. Particularly, the preferable boiling point and viscosity of the solvent is 0 — 40°C and 25 — 35 cps respectively. It is also important to maintain a proper temperature and humidity.
- a polymer and a low molecular compound included in the fibrous 3-dimensional polymer scaffold are dissolved in 5 — 20 weight% of an organic solvent to prepare a spinning solution.
- step (ii) a fiber is prepared by using the spinning solution with electro-spinner.
- Electric field is formed between nozzle and collector by applying a certain current from voltage generator.
- the polymer solution filled in the spinning solution depository is spun on the collector by the force of the electric field and the pressure from syringe pump.
- voltage, flowing speed, the electric field distance between nozzle and collector, temperature and humidity are important factors affecting spinning.
- concentration of the spinning solution affects the diameter of a fiber most significantly. So, all the conditions of the electro-spinner are optimized to prepare a fiber of the invention.
- the conditions of the electro-spinner are as follows; spinning distance: 10 — 20 cm, voltage: 10 — 20 kV and spinning speed: 0.050 — 0.150 ml/min, but not always limited thereto.
- the electro-spinner used in the present invention is DH High Voltage Generator (CPS-40KO3VIT, Chungpa EMT, Korea).
- the present invention further provides an implantation material for cell adhesion, growth and regeneration containing the fibrous porous 3-dimensional scaffold for tissue regeneration of the invention.
- the applicable cells are not limited but cartilage cells, endothelial cells, skin cells, osteocytes, bone cells and stem cells are preferred.
- Fig. 1 is a schematic diagram illustrating the spinning using an electro-spinner.
- Fig. 2 is a photomicrograph (X 500) of fiber prepared under the conditions of double electric field length: 20 cm, voltage: 10 V, release rate: 0.060 ml/min., and inner diameter of needle: 1.2 mm.
- Fig. 3 is a photomicrograph (X 3500) of fiber prepared under the conditions of double electric field length: 20 cm, voltage: 10 V, release rate: 0.060 ml/min., and inner diameter of needle: 1.2 mm.
- Fig. 4 is a photomicrograph (X 2000) showing the surface of the fibrous porous scaffold prepared by electrospinning under the conditions of double electric field length: 20 cm, voltage: 10 V, release rate: 0.060 ml/min., and inner diameter of needle: 1.2 mm.
- Fig. 5 is a photomicrograph(X 2000) showing osteoblasts cultured for 7 days in low molecular scaffold.
- Fig. 6 is a set of photomicrograph(X 500) showing osteoblasts cultured for 14 days in low molecular scaffold.
- Fig. 7 is appearance of electrospun PLLA sub-micro fibrous scaffold.
- A electrospun fibers
- B 3-D formed scaffold after handling electrospun fibers.
- Example 1 Preparation of a polymer PLLA fiber
- a PLLA polymer was dissolved in 10 D of dichloromethane solution, resulting in a
- a fiber was prepared from the spinning solution by electrospinning (Fig. 1).
- the prepared polymer PLLA fiber was confirmed to be 3 — 10 D in thickness.
- FIGs. 2 and 3 are photomicrographs (X 500, X 3500) of fibers prepared under the conditions of 20 cm of double electric field distance, 10 V of voltage, 0.060 ml/min of releasing speed and 1.2 mm of the internal diameter of a needle.
- a low molecular PLLA was dissolved in 10 D of dichloromethane solution, resulting in a 14 — 20% spinning solution.
- a fiber was prepared from the spinning solution by electrospinning (Fig. 1).
- the 14 — 20% low molecular PLLA solution (spinning solution) was filled in a spinning solution depository, which was a 10 D glass syringe. A needle, which is 0.5 — 1.2 mm in diameter, was used. The releasing speed of the spinning solution was adjusted to 0.060 ml/min. Voltage was set at 10 — 20 kV and the electric field distance was adjusted to 10 — 20 cm. It was important for the entire solvent to be volatilized before the drip of the solution on a collector to prepare a target fiber. Thus, the temperature and humidity had to be carefully regulated; the optimum temperature was 15 — 25°C and the optimum humidity was 10 — 40%.
- the prepared low molecular PLLA fiber was confirmed to be 5 — 10 D in thickness.
- Fig. 2 is a photomicrograph (X 2000) of a fiber prepared under the conditions of 10 cm of double electric field distance, 10 V of voltage, 0.060 ml/min of releasing speed and 1.2 mm of the internal diameter of a needle.
- the fibrous scaffolds prepared in Examples 1 and 2 were sterilized with 70% ethanol, on which sub-cultured osteoblasts (MC3TC) were static cultured. Observation on the adhered cells was performed under differential scanning microscope.
- MC3TC sub-cultured osteoblasts
- the prepared fiber was still stable in shape and in strength even after 7 days from the preparation and osteoblasts were packed between and on the surfaces of the fibers. Accordingly, it was confirmed that the porous scaffold of the present invention had cellular affinity, so that cells could be adhered stably. Therefore, the porous scaffold of the invention can be accepted as an appropriate scaffold material (Figs. 5, 6 and 7).
- the fibrous porous scaffold for tissue regeneration of the present invention has a biomimetic structure, which can be prepared by using electrospinning efficiently and with simple techniques.
- the fibrous porous scaffold for tissue regeneration of the invention has the size of between nanofiber and microfiber and a regular form and strength, so that it enables 3-dimensional regeneration of biological tissues and enhances porosity, suggesting that the cell-contacting surface area becomes large to facilitate cell adhesion, growth and regeneration.
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Dermatology (AREA)
- Transplantation (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Epidemiology (AREA)
- General Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Organic Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Materials Engineering (AREA)
- Composite Materials (AREA)
- Textile Engineering (AREA)
- Mechanical Engineering (AREA)
- Physical Education & Sports Medicine (AREA)
- Heart & Thoracic Surgery (AREA)
- Cardiology (AREA)
- Materials For Medical Uses (AREA)
- Spinning Methods And Devices For Manufacturing Artificial Fibers (AREA)
- Nonwoven Fabrics (AREA)
- Artificial Filaments (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2008527856A JP2009507530A (en) | 2005-08-26 | 2006-08-28 | Fiber type three-dimensional porous support for tissue regeneration using electrospinning and method for producing the same |
US12/064,801 US20080233162A1 (en) | 2005-08-26 | 2006-08-28 | Fibrous 3-Dimensional Scaffold Via Electrospinning For Tissue Regeneration and Method For Preparing the Same |
EP06798560A EP1917048A4 (en) | 2005-08-26 | 2006-08-28 | Fibrous 3-dimensional scaffold via electrospinning for tissue regeneration and method for preparing the same |
CA2621206A CA2621206C (en) | 2005-08-26 | 2006-08-28 | Fibrous 3-dimensional scaffold via electrospinning for tissue regeneration and method for preparing the same |
US14/047,948 US20210308335A1 (en) | 2005-08-26 | 2013-10-07 | Fibrous 3-Dimensional Scaffold Via Electrospinning For Tissue Regeneration and Method For Preparing the Same |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020050078640A KR100875189B1 (en) | 2005-08-26 | 2005-08-26 | Fibrous three-dimensional porous support for tissue regeneration using electrospinning and its preparation method |
KR10-2005-0078640 | 2005-08-26 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/KR2013/005094 Continuation-In-Part WO2013183976A1 (en) | 2005-08-26 | 2013-06-10 | Patch for tissue regeneration, comprising fibrous porous three-dimensional scaffold |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US12/064,801 A-371-Of-International US20080233162A1 (en) | 2005-08-26 | 2006-08-28 | Fibrous 3-Dimensional Scaffold Via Electrospinning For Tissue Regeneration and Method For Preparing the Same |
US14/047,948 Continuation-In-Part US20210308335A1 (en) | 2005-08-26 | 2013-10-07 | Fibrous 3-Dimensional Scaffold Via Electrospinning For Tissue Regeneration and Method For Preparing the Same |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2007024125A1 true WO2007024125A1 (en) | 2007-03-01 |
WO2007024125A9 WO2007024125A9 (en) | 2012-04-05 |
Family
ID=37771825
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/KR2006/003390 WO2007024125A1 (en) | 2005-08-26 | 2006-08-28 | Fibrous 3-dimensional scaffold via electrospinning for tissue regeneration and method for preparing the same |
Country Status (7)
Country | Link |
---|---|
US (1) | US20080233162A1 (en) |
EP (1) | EP1917048A4 (en) |
JP (1) | JP2009507530A (en) |
KR (1) | KR100875189B1 (en) |
CN (1) | CN101272814A (en) |
CA (1) | CA2621206C (en) |
WO (1) | WO2007024125A1 (en) |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1960513A2 (en) * | 2005-12-16 | 2008-08-27 | Cornell Research Foundation Inc. | Non-woven fabric for biomedical application based on poly (ester-amide)s |
WO2009102484A2 (en) * | 2008-02-14 | 2009-08-20 | Wake Forest University Health Sciences | Inkjet printing of tissues and cells |
US8524796B2 (en) | 2008-08-13 | 2013-09-03 | Dow Global Technologies Llc | Active polymer compositions |
WO2015026299A1 (en) * | 2013-08-23 | 2015-02-26 | National University Of Singapore | 3-dimensional bioscaffolds |
WO2015074631A1 (en) | 2013-11-21 | 2015-05-28 | Contipro Biotech S.R.O. | Voluminous nanofibrous material based on hyaluronic acid, its salt or their derivatives, method of preparation thereof, method of modification thereof, modified nanofibrous material, nanofibrous structure and use thereof |
KR101527469B1 (en) * | 2013-11-04 | 2015-06-11 | 연세대학교 산학협력단 | A Method for Fabrication of Porous fiber microstructure with various 3-dimensional Structures |
WO2016042211A1 (en) | 2014-09-17 | 2016-03-24 | University Of Helsinki | Implantable materials and uses thereof |
US9770529B2 (en) | 2008-12-12 | 2017-09-26 | The University Of Manchester | Tissue repair scaffold |
WO2017212102A1 (en) * | 2016-06-08 | 2017-12-14 | Universitat Politécnica De València | Non-woven bioelastomer composite |
CN108042854A (en) * | 2017-12-16 | 2018-05-18 | 诺迈尔(苏州)医学科技有限公司 | For the production technology of the gelatin fiber guide tissue regeneration film of Dental implant surgery |
CN114870075A (en) * | 2022-05-16 | 2022-08-09 | 东南大学 | Membrane for in-situ tissue regeneration enhancement and preparation method thereof |
Families Citing this family (43)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013183976A1 (en) * | 2012-06-08 | 2013-12-12 | 이화여자대학교 산학협력단 | Patch for tissue regeneration, comprising fibrous porous three-dimensional scaffold |
JP4777760B2 (en) * | 2005-12-01 | 2011-09-21 | 株式会社Snt | Composite structure including network structure |
JP5314298B2 (en) * | 2007-03-15 | 2013-10-16 | 太陽化学株式会社 | Electrospun composition |
KR100953366B1 (en) * | 2007-12-28 | 2010-04-20 | 한양대학교 산학협력단 | Nano fiber for tissue regeneration and fabrication method thereof |
KR101106244B1 (en) | 2009-05-01 | 2012-01-18 | 서울대학교산학협력단 | Electrospinning device and method for forming three dimensional nano structure |
CN101695585B (en) * | 2009-10-27 | 2013-02-20 | 吉林大学 | Degradation controlled tissue engineering cornea fibrous scaffold and preparation method thereof |
CN101891292B (en) * | 2010-07-27 | 2011-06-29 | 北京师范大学 | Method for removing trace polycyclic aromatic hydrocarbon from water through quick adsorption |
KR101226629B1 (en) * | 2010-12-08 | 2013-01-28 | 이화여자대학교 산학협력단 | Patch for tissue regeneration comprising fibrous 3-dimensional scaffold |
KR101328645B1 (en) | 2011-02-28 | 2013-11-14 | 주식회사 원바이오젠 | Nano/micro hybrid fiber non-woven fabric using biodegradable polymers and method for preparing the same |
EP2508212A1 (en) | 2011-04-05 | 2012-10-10 | Universitätsklinikum Freiburg | Biocompatible and biodegradable gradient layer system for regenerative medicine and for tissue support |
KR101380780B1 (en) * | 2012-02-09 | 2014-04-02 | 순천향대학교 산학협력단 | Fabrication Method of Bilayer Scaffold For Skin Tissue |
CN103572508B (en) * | 2012-07-26 | 2016-06-08 | 中国科学院理化技术研究所 | Preparation of biodegradable polymer nanofiber membrane by emulsion electrospinning method |
KR101998410B1 (en) * | 2012-08-22 | 2019-07-09 | 가톨릭대학교 산학협력단 | Scaffolds having double layer structure with gradient mineral concentration for tissue regeneration and preparation method thereof |
CN102908668B (en) * | 2012-11-09 | 2016-12-21 | 无锡中科光远生物材料有限公司 | The preparation method of growth induction form absorbable patch |
CN102949751A (en) * | 2012-11-28 | 2013-03-06 | 川北医学院第二临床医学院 | Preparation method for tissue engineering collagen-hyaluronic acid-chondroitin sulfate electrostatic spinning bracket |
KR101495595B1 (en) * | 2013-06-10 | 2015-03-03 | 안동대학교 산학협력단 | Three-dimensional hybrid scaffold manufacturing device |
CN103603138B (en) * | 2013-11-15 | 2016-06-01 | 无锡中科光远生物材料有限公司 | The preparation method of a kind of PLGA tunica fibrosa transplanted for cornea tissue |
KR101637070B1 (en) * | 2014-01-06 | 2016-07-06 | 안동대학교 산학협력단 | Nano-micro hybrid scaffold |
KR101449645B1 (en) * | 2014-04-08 | 2014-10-14 | 부산대학교 산학협력단 | POROUS ElECTROSPUN FIBER FOR POSSIBLE THICKNESS CONTROL, ITS MANUFACTURING METHOD, AND FOUR DIMENSIONAL SCAFFOLD FOR TISSUE REGENERATION USING THE SAME |
WO2015157485A1 (en) * | 2014-04-10 | 2015-10-15 | The Johns Hopkins University | Device and method for a nanofiber wrap to minimize inflamation and scarring |
WO2015199492A1 (en) * | 2014-06-27 | 2015-12-30 | 경북대학교 산학협력단 | Nano-fiber mat, method for manufacturing same, and use thereof as cell culture mat or guided bone regeneration shielding membrane |
KR101492771B1 (en) | 2014-06-27 | 2015-02-12 | 경북대학교 산학협력단 | Nanofibrous membrane for guided bone regeneration and method of manufacturing the same |
WO2016010377A1 (en) * | 2014-07-16 | 2016-01-21 | 부산대학교 산학협력단 | Biomimetic support for three-dimensional cell culturing, method for manufacturing same, and use thereof |
WO2016024720A1 (en) * | 2014-08-13 | 2016-02-18 | 박종철 | Mobile or portable electrospinning apparatus |
WO2017209521A1 (en) * | 2016-05-31 | 2017-12-07 | 주식회사 아모라이프사이언스 | Scaffold for cell culture or tissue engineering |
KR101927840B1 (en) * | 2016-06-13 | 2018-12-12 | 주식회사 아모그린텍 | Yarn for cell culture scaffold and fabic comprising the same |
CN105944145A (en) * | 2016-06-16 | 2016-09-21 | 浙江理工大学 | Preparation method of stent for promoting directional growth and migration of osteoblast |
CN106390208A (en) * | 2016-10-09 | 2017-02-15 | 华南理工大学 | Three-dimensional support material containing hierarchical porous structures and preparation and application |
CN107137764A (en) * | 2017-05-11 | 2017-09-08 | 芜湖扬展新材料科技服务有限公司 | A kind of preparation method of DOPA structural modification PLA tissue renovation material |
CN107469143A (en) * | 2017-08-28 | 2017-12-15 | 昆明医科大学第附属医院 | A kind of nano material for promoting bone cell growth and its preparation method and application |
CN107773785A (en) * | 2017-10-31 | 2018-03-09 | 无锡中科光远生物材料有限公司 | A kind of preparation method of cellular layer/tunica fibrosa compound film sheet for repairing bone defect |
SG11202012857PA (en) * | 2018-07-09 | 2021-02-25 | Nat Inst Materials Science | Nonwoven fabric, method for manufacturing same, and composition for electrospinning |
CN111793898A (en) * | 2019-04-09 | 2020-10-20 | 中国科学院大连化学物理研究所 | Nano cellulose membrane and preparation method thereof |
JP7276608B2 (en) * | 2020-06-02 | 2023-05-18 | 株式会社村田製作所 | Sheet comprising nanofibers and manufacturing method thereof |
CN111793900A (en) * | 2020-06-15 | 2020-10-20 | 中国人民解放军陆军特色医学中心 | Chitosan/polycaprolactone composite nanofiber membrane material and application thereof |
CN112144176A (en) * | 2020-09-01 | 2020-12-29 | 郑州大学 | Method for producing porous three-dimensional material by enzymatic degradation of PCL/PLLA polymer |
KR102563981B1 (en) * | 2021-01-29 | 2023-08-07 | 전북대학교산학협력단 | Artificial lumen and method for manufacturing thereof |
CN113373543A (en) * | 2021-07-20 | 2021-09-10 | 广州医科大学附属第五医院 | Method for regulating bead appearance in beaded nanofiber |
CN113790958B (en) * | 2021-09-01 | 2024-06-14 | 沈阳恒生医用科技有限公司 | Polymer bionic tissue and application thereof |
CN114225106B (en) * | 2021-12-23 | 2023-03-21 | 广东工业大学 | Porous nanofiber biological membrane and preparation method and application thereof |
CN115282334A (en) * | 2022-01-10 | 2022-11-04 | 上海市第六人民医院 | Piezoelectric type amino acid biological scaffold and preparation method thereof |
CN114438782B (en) * | 2022-03-24 | 2023-05-02 | 新疆师范大学 | Preparation method of polylactic acid/tannic acid/iron/cysteine osteoinduction composite fiber membrane with rapid antibacterial/antioxidant activity |
CN115094529A (en) * | 2022-07-11 | 2022-09-23 | 吉林大学第一医院 | Porous orientation PLGA electrostatic spinning fiber and preparation method thereof |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999018893A1 (en) * | 1997-10-10 | 1999-04-22 | Drexel University | Hybrid nanofibril matrices for use as tissue engineering devices |
KR20040016320A (en) * | 2002-08-16 | 2004-02-21 | (주)삼신크리에이션 | Electrospinning apparatus for producing nanofiber and electrospinning nozzle pack for the same |
US6753454B1 (en) * | 1999-10-08 | 2004-06-22 | The University Of Akron | Electrospun fibers and an apparatus therefor |
KR20050040186A (en) * | 2003-10-28 | 2005-05-03 | 이승진 | Fibrous porous scaffold comprising biodegradable polymer and the method thereof |
KR20050040187A (en) * | 2003-10-28 | 2005-05-03 | 이승진 | Nano-microfibrous scaffold for enhanced tissue regeneration and method for preparing the same |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100336700B1 (en) * | 1999-05-12 | 2002-05-13 | 오석송 | Biodegradable guided tissue regeneration in teriodontal dental therapy and methods of producing the same |
US7041868B2 (en) * | 2000-12-29 | 2006-05-09 | Kimberly-Clark Worldwide, Inc. | Bioabsorbable wound dressing |
KR20020063020A (en) * | 2001-01-26 | 2002-08-01 | 한국과학기술연구원 | Method for Preparing Thin Fiber -Structured Polymer Webs |
WO2003072748A2 (en) * | 2002-02-22 | 2003-09-04 | University Of Washington | Bioengineered tissue substitutes |
KR100491705B1 (en) * | 2002-07-30 | 2005-05-27 | 민병무 | Wound dressing of silk fibroin nanofibers nonwoven and its preparation |
TW200427889A (en) * | 2003-03-31 | 2004-12-16 | Teijin Ltd | Non-woven fabric and process for producing the same |
US7704740B2 (en) * | 2003-11-05 | 2010-04-27 | Michigan State University | Nanofibrillar structure and applications including cell and tissue culture |
JP4526851B2 (en) * | 2004-03-31 | 2010-08-18 | 明彦 谷岡 | Polysaccharide nanoscale fibers and compacts |
WO2006028244A1 (en) * | 2004-09-07 | 2006-03-16 | Teijin Limited | Bioabsorbable porous object |
KR100762928B1 (en) * | 2004-10-29 | 2007-10-04 | 재단법인서울대학교산학협력재단 | Nonwoven Nanofibrous Membranes of Silk Fibroin for Guided Bone Tissue Regeneration and Their Preparation Method |
-
2005
- 2005-08-26 KR KR1020050078640A patent/KR100875189B1/en not_active IP Right Cessation
-
2006
- 2006-08-28 CA CA2621206A patent/CA2621206C/en active Active
- 2006-08-28 JP JP2008527856A patent/JP2009507530A/en active Pending
- 2006-08-28 EP EP06798560A patent/EP1917048A4/en not_active Withdrawn
- 2006-08-28 CN CNA2006800350386A patent/CN101272814A/en active Pending
- 2006-08-28 WO PCT/KR2006/003390 patent/WO2007024125A1/en active Application Filing
- 2006-08-28 US US12/064,801 patent/US20080233162A1/en not_active Abandoned
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999018893A1 (en) * | 1997-10-10 | 1999-04-22 | Drexel University | Hybrid nanofibril matrices for use as tissue engineering devices |
US6753454B1 (en) * | 1999-10-08 | 2004-06-22 | The University Of Akron | Electrospun fibers and an apparatus therefor |
KR20040016320A (en) * | 2002-08-16 | 2004-02-21 | (주)삼신크리에이션 | Electrospinning apparatus for producing nanofiber and electrospinning nozzle pack for the same |
KR20050040186A (en) * | 2003-10-28 | 2005-05-03 | 이승진 | Fibrous porous scaffold comprising biodegradable polymer and the method thereof |
KR20050040187A (en) * | 2003-10-28 | 2005-05-03 | 이승진 | Nano-microfibrous scaffold for enhanced tissue regeneration and method for preparing the same |
Non-Patent Citations (2)
Title |
---|
See also references of EP1917048A4 * |
YANG F. ET AL.: "Electrospinning of nano/micro scale poly(L-lactic acid) aligned fibers and their potential in neural tissue engineering", BIOMATERIALS, vol. 26, no. 15, May 2005 (2005-05-01), pages 2603 - 2610, XP004673425 * |
Cited By (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1960513A4 (en) * | 2005-12-16 | 2009-04-22 | Cornell Res Foundation Inc | Non-woven fabric for biomedical application based on poly (ester-amide)s |
EP1960513A2 (en) * | 2005-12-16 | 2008-08-27 | Cornell Research Foundation Inc. | Non-woven fabric for biomedical application based on poly (ester-amide)s |
US9301925B2 (en) | 2008-02-14 | 2016-04-05 | Wake Forest University Health Sciences | Inkjet printing of tissues and cells |
WO2009102484A2 (en) * | 2008-02-14 | 2009-08-20 | Wake Forest University Health Sciences | Inkjet printing of tissues and cells |
WO2009102484A3 (en) * | 2008-02-14 | 2009-12-03 | Wake Forest University Health Sciences | Inkjet printing of tissues and cells |
US8691274B2 (en) | 2008-02-14 | 2014-04-08 | Wake Forest University Health Sciences | Inkjet printing of tissues and cells |
US9005972B2 (en) | 2008-02-14 | 2015-04-14 | Wake Forest University Health Sciences | Inkjet printing of tissues and cells |
US8524796B2 (en) | 2008-08-13 | 2013-09-03 | Dow Global Technologies Llc | Active polymer compositions |
US9770529B2 (en) | 2008-12-12 | 2017-09-26 | The University Of Manchester | Tissue repair scaffold |
WO2015026299A1 (en) * | 2013-08-23 | 2015-02-26 | National University Of Singapore | 3-dimensional bioscaffolds |
KR101527469B1 (en) * | 2013-11-04 | 2015-06-11 | 연세대학교 산학협력단 | A Method for Fabrication of Porous fiber microstructure with various 3-dimensional Structures |
WO2015074631A1 (en) | 2013-11-21 | 2015-05-28 | Contipro Biotech S.R.O. | Voluminous nanofibrous material based on hyaluronic acid, its salt or their derivatives, method of preparation thereof, method of modification thereof, modified nanofibrous material, nanofibrous structure and use thereof |
WO2016042211A1 (en) | 2014-09-17 | 2016-03-24 | University Of Helsinki | Implantable materials and uses thereof |
WO2017212102A1 (en) * | 2016-06-08 | 2017-12-14 | Universitat Politécnica De València | Non-woven bioelastomer composite |
CN108042854A (en) * | 2017-12-16 | 2018-05-18 | 诺迈尔(苏州)医学科技有限公司 | For the production technology of the gelatin fiber guide tissue regeneration film of Dental implant surgery |
CN114870075A (en) * | 2022-05-16 | 2022-08-09 | 东南大学 | Membrane for in-situ tissue regeneration enhancement and preparation method thereof |
Also Published As
Publication number | Publication date |
---|---|
KR20070024092A (en) | 2007-03-02 |
WO2007024125A9 (en) | 2012-04-05 |
US20080233162A1 (en) | 2008-09-25 |
CN101272814A (en) | 2008-09-24 |
CA2621206A1 (en) | 2007-03-01 |
EP1917048A4 (en) | 2012-07-18 |
JP2009507530A (en) | 2009-02-26 |
KR100875189B1 (en) | 2008-12-19 |
EP1917048A1 (en) | 2008-05-07 |
CA2621206C (en) | 2011-11-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CA2621206C (en) | Fibrous 3-dimensional scaffold via electrospinning for tissue regeneration and method for preparing the same | |
Wu et al. | Resorbable polymer electrospun nanofibers: History, shapes and application for tissue engineering | |
US20220162412A1 (en) | Three-dimensional porous hybrid scaffold and manufacture thereof | |
Sokolsky-Papkov et al. | Polymer carriers for drug delivery in tissue engineering | |
Zhang et al. | Nanofiber-based delivery of bioactive agents and stem cells to bone sites | |
Martins et al. | Electrospinning: processing technique for tissue engineering scaffolding | |
Hoque et al. | Gelatin based scaffolds for tissue engineering-a review | |
Venugopal et al. | Interaction of cells and nanofiber scaffolds in tissue engineering | |
Chung et al. | Surface engineered and drug releasing pre-fabricated scaffolds for tissue engineering | |
Vasita et al. | Nanofibers and their applications in tissue engineering | |
CN101507835B (en) | Nano bionic wound-surface cover and preparation method thereof | |
Ashammakhi et al. | Advancing tissue engineering by using electrospun nanofibers | |
Piskin et al. | Electrospun matrices made of poly (α-hydroxy acids) for medical use | |
KR100621569B1 (en) | Nano-microfibrous scaffold for enhanced tissue regeneration and method for preparing the same | |
Ribba et al. | Electrospun nanofibrous mats: from vascular repair to osteointegration | |
de Lima et al. | Electrospinning of hydrogels for biomedical applications | |
Shi et al. | Preparation of porous polylactide microspheres and their application in tissue engineering | |
Hoque et al. | Electrospun matrices from natural polymers for skin regeneration | |
Del Bakhshayesh et al. | High efficiency biomimetic electrospun fibers for use in regenerative medicine and drug delivery: A review | |
KR20150007808A (en) | Complex scaffold comprising nanofiber with nanoparticle to drug-delivery for artificial skin and filler, and method for preparing the same | |
CN111603609B (en) | Bionic tissue engineering scaffold and preparation method thereof | |
Carter et al. | Engineered Biomimicry: Chapter 7. Bioscaffolds: Fabrication and Performance | |
US20130280307A1 (en) | Encapsulation of matter in polymer structures | |
Xu et al. | Electrospun three-dimensional nanofibrous scaffolds based on polycaprolactone for stem cells differentiation and bone regeneration | |
US20210162096A1 (en) | Matrices for tissue engineering in the form of foams, fibres and/or membranes formed of polymers, ceramics, polymeric composites and/or ceramic composites containing bixa orellana l. extract and method of production |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
WWE | Wipo information: entry into national phase |
Ref document number: 200680035038.6 Country of ref document: CN |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
WWE | Wipo information: entry into national phase |
Ref document number: 12064801 Country of ref document: US |
|
ENP | Entry into the national phase |
Ref document number: 2621206 Country of ref document: CA |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2008527856 Country of ref document: JP |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2006798560 Country of ref document: EP |