CN102908668B - The preparation method of growth induction form absorbable patch - Google Patents
The preparation method of growth induction form absorbable patch Download PDFInfo
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- CN102908668B CN102908668B CN201210444940.0A CN201210444940A CN102908668B CN 102908668 B CN102908668 B CN 102908668B CN 201210444940 A CN201210444940 A CN 201210444940A CN 102908668 B CN102908668 B CN 102908668B
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Abstract
The present invention relates to the preparation method of a kind of growth induction form absorbable patch, by PCL, PLGA, Type three kinds of components of A low viscosity gelatin, prepared the nano-fiber material with composite performance by solution blending spinning or spinning head co-blended spinning；Either solution blending spin processes or spinning head co-blended spinning method all can obtain the composite that three components are well combined, and each component can be good at keeping the performance of its body so that composite has multifunctionality；The method simple, device simple, low cost novel in design, it is possible to provide there is the macromolecular material of property, and can be used for large-scale production.
Description
Technical field:
The present invention relates to degradable high polymer material and field of compound material, be specifically related to a kind of growth induction form absorbable
The preparation method of sticking patch.
Background technology:
The current demand for organ transplantation and tissue transplantation is considerably beyond quantity delivered, and this gap is also gradually
Widen.Organizational project is a kind of reconstruction and repair deficiency or a kind of new method of damaged tissues, and this is a kind of by life sciences
The multi-crossed disciplines science that engineering combines with artificial natural materials and environmental condition.Organizational project has three critically important
Parameter: cell, support and cell be the condition of three dimensional growth on support, in life sciences, support is the growing into of cell, breeds
And formation new organization provides a three-dimensional template framing structure.In organizational project, preferable support Design needs to meet several
Part: the highest porosity and suitable aperture, the space of support plays an important role for the propagation of cell, and cell adhesion exists
Rack surface, is absorbed nutrient substance, is then transported away by metabolite by space, and the diameter of cell determines minimum-value aperture
Size, the diameter of cell is different along with the difference of cell, so the size in aperture also must strictly control, as
Really hole too minicell just cannot pass through, and too maxicell just cannot adhere to；The highest specific surface area so that cell adhesion, growth,
Transfer and differentiation；3. the necessary degradable of support, degradation rate need to match with the speed that tissue increases；4. biocompatibility,
Frame is no matter in bulk state or all do not have toxicity again in degradation process to cell；5. support must have enough mechanical properties
Support cambium.Develop a kind of material meeting above-mentioned requirements and become people's urgent problem.
Summary of the invention:
It is an object of the invention to provide the preparation method of a kind of growth induction form absorbable patch.
To achieve these goals, the present invention propose following technical scheme realize:
Growth induction form absorbable patch, it is characterised in that: this sticking patch is based on first group of degradable high polymer material
Material, and add second group of degradable high polymer material, gelatin, use electrostatic spinning technique to make.
Preparation method thickness 60 μm of described growth induction form absorbable patch～500 μm.
First group of described degradable high polymer material is PCL, number-average molecular weight 10,000～400,000.
Second group of described degradable high polymer material is PLGA, number-average molecular weight 30,000～500,000, and segment mole
Than L: G=90: 10～40: 60.
Described gelatin or collagen protein select Type A low viscosity gelatin or low viscosity collagen protein.
The preparation method of growth induction form absorbable patch, comprises the steps:
(1) preparation macromolecular solution: first group of degradable high polymer material is dissolved in corresponding cosolvent, is configured to concentration
It is the solution of 10～60wt%；Second group of degradable high polymer material is dissolved in corresponding cosolvent to gelatin, is configured to concentration 5
～the solution of 50wt%, wherein second group of degradable high polymer material accounts for the 80%～99% of gross mass, and gelatin accounts for gross mass
1%～20%；
Or first group of degradable high polymer material, second group of degradable high polymer material, gelatin are dissolved in its cosolvent,
Being configured to the solution of 10～60wt%, wherein second group of degradable high polymer material accounts for first group of degradable high polymer material quality
The 10～90% of mark, gelatin accounts for the 1-20% of first group of degradable high polymer material mass fraction；
(2) electrostatic spinning: controlling spinning environment temperature is 20～30 DEG C, the first group of degradable macromolecule (1) obtained
The solution of the solution of material, second group of degradable high polymer material and gelatin is respectively placed in two feeds notes of electrospinning device
In emitter, arranging the quantitative proportion of spinning syringe needle between two kinds of solution is 10: 9～7: 3, and spinning a to roller receives on device；
Or obtain in (1) first group of degradable high polymer material, second group of degradable high polymer material, mixed solution of gelatin are put
In the feed injector of electrospinning device, spinning to roller receives on device；Distance between regulation spinning head and roller
Being 7～15cm, the air velocity in environment controls 0.5～0.8m3/ hr, opens high voltage power supply and feed injector pump, adjusts
Economize on electricity is depressed into 10～35KV, and the delivery rate of solution is 10～30 μ l/min, obtains electrospun fibers on a rotating drum multiple
Close film.
Described cosolvent is DMF, acetone, trifluoroethanol, hexafluoroisopropanol, THF, dimethyl acetylamide, dichloromethane
In one or more.
Described electrospinning device is many spinning heads electrostatic spinning machine.
Beneficial effects of the present invention: by PCL, PLGA, Type three kinds of components of A low viscosity gelatin, by solution blending spinning
Or spinning head co-blended spinning prepares the nano-fiber material with composite performance；Either solution blending spin processes or spray webbing
Head co-blended spinning method all can obtain the composite that three components are well combined, and each component can be good at keeping its body
Performance is so that composite has multifunctionality；The method simple, device simple, low cost novel in design, it is possible to provide tool
Have specific characteristics can macromolecular material, and can be used for large-scale production.
Detailed description of the invention:
Hereinafter describe the preferred embodiment of the present invention, but be not limited to the present invention.
Embodiment 1:
It is prepared as follows growth induction form absorbable patch:
(1) preparation of solution: by PCL (molecular weight 250000), PLGA (molecular weight 60000) and Type A low viscosity gelatin
Mixing is dissolved in hexafluoroisopropanol, is configured to the electrospun solution that concentration is 25wt%, and wherein PCL: PLGA: Type A low viscosity is bright
Glue=85: 15: 2；
(2) electrostatic spinning: the mixed solution that (1) obtains is placed in the feed injector of many spinning heads electrostatic spinning machine,
Controlling spinning environment temperature is 25 DEG C, and the distance between regulation spinning head and roller is 7～15cm, the air velocity control in environment
System is 0.5～0.8m3/hr；Open high voltage power supply and feed injector pump, regulation voltage to 10～35KV, the feed of solution
Speed is 10～30 μ l/min, obtains electrospun fibers composite membrane on a rotating drum.
Embodiment 2:
It is prepared as follows growth induction form absorbable patch:
(1) preparation of solution: be dissolved in trifluoroethanol by PCL (molecular weight 150000), being configured to PCL concentration is 20wt%
Electrospun solution；PLGA (molecular weight 60000) and Type A low viscosity gelatin is mixed and is dissolved in hexafluoroisopropanol, be configured to dense
Degree is the electrospun solution of 40wt%, wherein PLGA: Type A low viscosity gelatin=90: 10；
(2) electrostatic spinning: the PCL solution and PLGA, Type A low viscosity gelatin mixed solution that (1) are obtained are respectively placed in
In two feed injectors of many spinning heads electrostatic spinning machine, PCL solution is set and the mixing of PLGA, Type A low viscosity gelatin is molten
The quantitative proportion of liquid spinning syringe needle is 9: 1, and spinning a to roller receives on device, and controlling spinning environment temperature is 25 DEG C, adjusts
Distance between joint spinning head and roller is 7～15cm, and the air velocity in environment controls 0.5～0.8m3/hr；Open height
Voltage source and feed injector pump, regulation voltage is to 10～35KV, and the delivery rate of solution is 10～30 μ l/min, is rotating
Electrospun fibers composite membrane is obtained on roller.
Claims (2)
1. the preparation method of growth induction form absorbable patch, it is characterised in that:
(1) preparation macromolecular solution: being dissolved in corresponding cosolvent by first group of degradable high polymer material, being configured to concentration is 10
～the solution of 60wt%；Second group of degradable high polymer material is dissolved in corresponding cosolvent to gelatin, be configured to concentration 5～
The solution of 50wt%, wherein second group of degradable high polymer material accounts for the 80%～99% of gross mass, and gelatin accounts for the 1% of gross mass
～20%；
(2) electrostatic spinning: controlling spinning environment temperature is 20～30 DEG C, the first group of degradable high polymer material (1) obtained
Solution, the solution of second group of degradable high polymer material and gelatin be respectively placed in two feed injectors of electrospinning device
In, arranging the quantitative proportion of spinning syringe needle between two kinds of solution is 10: 9～7: 3, and spinning a to roller receives on device；Regulation
Distance between spinning head and roller is 7～15cm, and the air velocity in environment controls 0.5～0.8m3/ hr, opens high pressure
Power supply and feed injector pump, regulation voltage is to 10～35KV, and the delivery rate of solution is 10～30 μ l/min, is rotating rolling
Electrospun fibers composite membrane is obtained on Tong；First group of described degradable high polymer material is PCL, number-average molecular weight 10,000
～400,000；Second group of described degradable high polymer material is PLGA, number-average molecular weight 30,000～500,000, and segment rubs
You are than L: G=90: 10～40: 60；Described cosolvent is DMF, acetone, trifluoroethanol, THF, dimethyl acetylamide, dichloromethane
One or more in alkane.
The preparation method of growth induction form absorbable patch the most according to claim 1, it is characterised in that: described electrostatic
Spinning equipment is many spinning heads electrostatic spinning machine.
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| CN201210444940.0A CN102908668B (en) | 2012-11-09 | 2012-11-09 | The preparation method of growth induction form absorbable patch |
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| CN103603087B (en) * | 2013-11-15 | 2015-07-15 | 无锡中科光远生物材料有限公司 | Preparation method of nanofiber for soft tissue regeneration |
| CN104068956B (en) * | 2014-07-09 | 2017-04-26 | 广州医科大学 | High-strength degradable bone fracture binding band and preparation method thereof |
| US10463469B2 (en) * | 2015-08-06 | 2019-11-05 | Gunze Limited | Artificial blood vessel, method for producing artificial blood vessel, and method for producing porous tissue regeneration substrate |
| CN105233345A (en) * | 2015-08-25 | 2016-01-13 | 上海交通大学医学院附属仁济医院 | Natural protein/polycaprolactone nanofiber electrospun membrane, and preparation and application thereof |
| CN117883216A (en) * | 2024-01-19 | 2024-04-16 | 常州集硕医疗器械有限公司 | Bioabsorbable rotator cuff patch and preparation method thereof |
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| EP1804844A4 (en) * | 2004-09-29 | 2012-02-29 | Univ Singapore | A composite, method of producing the composite and uses of the same |
| KR100875189B1 (en) * | 2005-08-26 | 2008-12-19 | 이화여자대학교 산학협력단 | Fibrous three-dimensional porous support for tissue regeneration using electrospinning and its preparation method |
| CN1961974B (en) * | 2005-11-09 | 2010-04-21 | 中国科学院化学研究所 | Nano copolymer fibrous membrane material capable of being biodegraded and absorbed and preparation process and use thereof |
| KR101080203B1 (en) * | 2009-02-18 | 2011-11-07 | 부산대학교 산학협력단 | medical skin-patch fabricated by using multilayer nanofiber sheet |
| CN101507843B (en) * | 2009-03-20 | 2012-11-21 | 中国人民解放军第三军医大学 | Multi-purpose surgery biology patching material |
| CN101693024A (en) * | 2009-10-20 | 2010-04-14 | 中国科学院化学研究所 | Radionuclide-labelled biodegradable bioabsorbable biopolymer nano fibrous membrane, preparation process and application thereof |
| CN102552976A (en) * | 2012-02-20 | 2012-07-11 | 汪泱 | Tissue engineering bracket material capable of physically embedding active substances and preparation method thereof |
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