CN102908668A - Preparation method of induced growth type absorbable patch - Google Patents
Preparation method of induced growth type absorbable patch Download PDFInfo
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- CN102908668A CN102908668A CN2012104449400A CN201210444940A CN102908668A CN 102908668 A CN102908668 A CN 102908668A CN 2012104449400 A CN2012104449400 A CN 2012104449400A CN 201210444940 A CN201210444940 A CN 201210444940A CN 102908668 A CN102908668 A CN 102908668A
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Abstract
The invention relates to a preparation method of an induced growth type absorbable patch. The preparation method comprises the following step: preparing a nano-fiber material with composite performance from PCL (polycaprolactone), PLGA (poly (D,L-lactic-glycolic)) and Type A low-viscosity gelatin through liquid blending spinning or spinneret blending spinning. The composite material with excellent combination of three components can be prepared by either the liquid blending spinning method or the spinneret blending spinning method, and each component can maintain the performance of the component body, so that the composite material is multifunctional; and the method is novel and simple in design, easy in equipment and low in cost, can provide the polymer material with special functions, and can be used in scale production.
Description
Technical field:
The present invention relates to degradable high polymer material and field of compound material, be specifically related to the preparation method that a kind of induced growth type can absorb sticking patch.
Background technology:
At present for the demand of organ transplantation and tissue transplantation considerably beyond quantity delivered, and this gap is also widening gradually.Organizational project is a kind of new method of a kind of reconstruction and repair deficiency or damaged tissues, and this is a kind of multidisciplinary interdisciplinary science that life science engineering is combined with artificial natural materials and environmental condition.Three very important parameters are arranged in organizational project: the condition of cell, support and cell three dimensional growth on support, in life sciences, support provides a three-dimensional template framing structure for the new organization of growing into, breed and form of cell.Support Design desirable in organizational project need to satisfy several conditions: 1. high porosity and suitable aperture, the space of support plays an important role for the propagation of cell, cell adhesion is at rack surface, absorb nutrient substance, then by the space, metabolite is transported away, the diameter of cell has determined the size of minimum-value aperture, the diameter of cell is different along with the difference of cell, so the size in aperture also must strictly be controlled, if hole too minicell just can't be passed through, too maxicell just can't adhere to; 2. high specific surface area is so that cell adhesion, growth, transfer and differentiation; 3. support must degradable, and the speed that degradation rate needs to increase with tissue be complementary; 4. biocompatibility, no matter support does not still all have toxicity to cell in degradation process again in bulk state; 5. support must have enough mechanical property support cambiums.Develop a kind of material that meets above-mentioned requirements and become people's urgent problem.
Summary of the invention:
The object of the present invention is to provide a kind of induced growth type can absorb the preparation method of sticking patch.
To achieve these goals, the present invention proposes following technical scheme realization:
The induced growth type can absorb sticking patch, it is characterized in that: this sticking patch is take first group of degradable high polymer material as material of main part, and adds second group of degradable high polymer material, gelatin, adopts electrostatic spinning technique to make.
Described induced growth type can absorb the preparation method thickness 60 μ m of sticking patch～500 μ m.
Described first group of degradable high polymer material is PCL, number-average molecular weight 10,000～400,000.
Described second group of degradable high polymer material is PLGA, number-average molecular weight 30,000～500,000, segment mol ratio L: G=90: 10～40: 60.
Described gelatin or collagen protein are selected Type A low viscosity gelatin or low viscosity collagen protein.
The induced growth type can absorb the preparation method of sticking patch, comprises the steps:
(1) preparation macromolecular solution: first group of degradable high polymer material is dissolved in corresponding cosolvent, is mixed with the solution that concentration is 10～60wt%; Second group of degradable high polymer material and gelatin are dissolved in corresponding cosolvent, are mixed with the solution of concentration 5～50wt%, wherein second group of degradable high polymer material accounts for 80%～99% of gross mass, and gelatin accounts for 1%～20% of gross mass;
Or first group of degradable high polymer material, second group of degradable high polymer material, gelatin are dissolved in its cosolvent, be mixed with the solution of 10～60wt%, wherein second group of degradable high polymer material accounts for 10～90% of first group of degradable high polymer material mass fraction, and gelatin accounts for the 1-20% of first group of degradable high polymer material mass fraction;
(2) electrostatic spinning: controlling the spinning ambient temperature is 20～30 ℃, the solution of the solution of first group of degradable high polymer material that (1) is obtained, second group of degradable high polymer material and gelatin is placed in respectively in two feed injectors of electrospinning device, the quantitative proportion that spinning syringe needle between two kinds of solution is set is 10: 9～7: 3, on spinning to a roller receiving system; Or the mixed solution of first group of degradable high polymer material obtaining in (1), second group of degradable high polymer material, gelatin is placed in the feed injector of electrospinning device, spinning is to the roller receiving system; The distance of regulating between spinning head and roller is 7～15cm, and the air velocity in environment is controlled at 0.5～0.8m
3/ hr opens high voltage power supply and feed injector pump, regulation voltage to 10～35KV, and the delivery rate of solution is 10～30 μ l/min, obtains the electrospun fibers composite membrane on swing roller.
Described cosolvent is one or more in DMF, acetone, trifluoroethanol, hexafluoroisopropanol, THF, dimethyl acetylamide, dichloromethane.
Described electrospinning device is many spinning heads electrostatic spinning machine.
Beneficial effect of the present invention: with PCL, PLGA, three kinds of components of Type A low viscosity gelatin, prepare the nano-fiber material with composite performance by solution blending spinning or spinning head co-blended spinning; No matter be that solution blending spin processes or spinning head co-blended spinning method all can obtain three components in conjunction with good composite, thereby and each component can be good at keeping the performance of its body to make composite have multifunctionality; The method is novel in design simple, device simple, cost are low, the macromolecular material with property can be provided, and can be used for large-scale production.
The specific embodiment:
Below describe the preferred embodiment of the present invention, but be not to limit the present invention.
Embodiment 1:
Be prepared as follows the induced growth type and can absorb sticking patch:
(1) preparation of solution: PCL (molecular weight 250000), PLGA (molecular weight 60000) and the mixing of Type A low viscosity gelatin are dissolved in hexafluoroisopropanol, being mixed with concentration is the electrospinning solution of 25wt%, wherein PCL: PLGA: Type A low viscosity gelatin=85: 15: 2;
(2) electrostatic spinning: the mixed solution that (1) is obtained is placed in the feed injector of many spinning heads electrostatic spinning machine, controlling the spinning ambient temperature is 25 ℃, the distance of regulating between spinning head and roller is 7～15cm, and the air velocity in environment is controlled at 0.5～0.8m
3/ hr; Open high voltage power supply and feed injector pump, regulation voltage to 10～35KV, the delivery rate of solution is 10～30 μ l/min, obtains the electrospun fibers composite membrane on swing roller.
Embodiment 2:
Be prepared as follows the induced growth type and can absorb sticking patch:
(1) preparation of solution: PCL (molecular weight 150000) is dissolved in trifluoroethanol, and being mixed with PCL concentration is the electrospinning solution of 20wt%; PLGA (molecular weight 60000) is mixed with Type A low viscosity gelatin be dissolved in hexafluoroisopropanol, being mixed with concentration is the electrospinning solution of 40wt%, wherein PLGA: Type A low viscosity gelatin=90: 10;
(2) electrostatic spinning: PCL solution and PLGA, Type A low viscosity gelatin mixed solution that (1) is obtained are placed in respectively in two feed injectors of many spinning heads electrostatic spinning machine, the quantitative proportion that PCL solution and PLGA, Type A low viscosity gelatin mixed solution spinning syringe needle are set is 9: 1, on spinning to a roller receiving system, controlling the spinning ambient temperature is 25 ℃, the distance of regulating between spinning head and roller is 7～15cm, and the air velocity in environment is controlled at 0.5～0.8m
3/ hr; Open high voltage power supply and feed injector pump, regulation voltage to 10～35KV, the delivery rate of solution is 10～30 μ l/min, obtains the electrospun fibers composite membrane on rotating drum.
Claims (6)
1. the induced growth type can absorb the preparation method of sticking patch, it is characterized in that:
(1) preparation macromolecular solution: first group of degradable high polymer material is dissolved in corresponding cosolvent, is mixed with the solution that concentration is 10～60wt%; Second group of degradable high polymer material and gelatin are dissolved in corresponding cosolvent, are mixed with the solution of concentration 5～50wt%, wherein second group of degradable high polymer material accounts for 80%～99% of gross mass, and gelatin accounts for 1%～20% of gross mass;
Or first group of degradable high polymer material, second group of degradable high polymer material, gelatin are dissolved in its cosolvent, be mixed with the solution of 10～60wt%, wherein second group of degradable high polymer material accounts for 10～90% of first group of degradable high polymer material mass fraction, and gelatin accounts for the 1-20% of first group of degradable high polymer material mass fraction;
(2) electrostatic spinning: controlling the spinning ambient temperature is 20～30 ℃, the solution of the solution of first group of degradable high polymer material that (1) is obtained, second group of degradable high polymer material and gelatin is placed in respectively in two feed injectors of electrospinning device, the quantitative proportion that spinning syringe needle between two kinds of solution is set is 10: 9～7: 3, on spinning to a roller receiving system; Or the mixed solution of first group of degradable high polymer material obtaining in (1), second group of degradable high polymer material, gelatin is placed in the feed injector of electrospinning device, spinning is to the roller receiving system; The distance of regulating between spinning head and roller is 7～15cm, and the air velocity in environment is controlled at 0.5～0.8m
3/ hr opens high voltage power supply and feed injector pump, regulation voltage to 10～35KV, and the delivery rate of solution is 10～30 μ l/min, obtains the electrospun fibers composite membrane on swing roller.
2. induced growth type according to claim 1 can absorb the preparation method of sticking patch, it is characterized in that: described first group of degradable high polymer material is PCL, number-average molecular weight 10,000～400,000.
3. induced growth type according to claim 1 can absorb the preparation method of sticking patch, it is characterized in that: described second group of degradable high polymer material is PLGA, number-average molecular weight 30,000～500,000, segment mol ratio L: G=90: 10～40: 60.
4. induced growth type according to claim 1 can absorb the preparation method of sticking patch, it is characterized in that: described gelatin or collagen protein are selected Type A low viscosity gelatin or low viscosity collagen protein.
5. induced growth type according to claim 1 can absorb the preparation method of sticking patch, it is characterized in that: described cosolvent is one or more in DMF, acetone, trifluoroethanol, hexafluoroisopropanol, THF, dimethyl acetylamide, dichloromethane.
6. induced growth type according to claim 1 can absorb the preparation method of sticking patch, it is characterized in that: described electrospinning device is many spinning heads electrostatic spinning machine.
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Cited By (4)
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| CN103603087A (en) * | 2013-11-15 | 2014-02-26 | 无锡中科光远生物材料有限公司 | Preparation method of nanofiber for soft tissue regeneration |
| CN104068956A (en) * | 2014-07-09 | 2014-10-01 | 广州医科大学 | High-strength degradable bone fracture binding band and preparation method thereof |
| CN105233345A (en) * | 2015-08-25 | 2016-01-13 | 上海交通大学医学院附属仁济医院 | Natural protein/polycaprolactone nanofiber electrospun membrane, and preparation and application thereof |
| CN107735114A (en) * | 2015-08-06 | 2018-02-23 | 郡是株式会社 | The manufacture method of artificial blood vessel, the manufacture method of artificial blood vessel and Porous regeneration base material |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104068956A (en) * | 2014-07-09 | 2014-10-01 | 广州医科大学 | High-strength degradable bone fracture binding band and preparation method thereof |
| CN107735114A (en) * | 2015-08-06 | 2018-02-23 | 郡是株式会社 | The manufacture method of artificial blood vessel, the manufacture method of artificial blood vessel and Porous regeneration base material |
| CN107735114B (en) * | 2015-08-06 | 2020-11-10 | 郡是株式会社 | Artificial blood vessel, method for producing artificial blood vessel, and method for producing porous tissue regeneration substrate |
| CN105233345A (en) * | 2015-08-25 | 2016-01-13 | 上海交通大学医学院附属仁济医院 | Natural protein/polycaprolactone nanofiber electrospun membrane, and preparation and application thereof |
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| CN102908668B (en) | 2016-12-21 |
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Effective date of registration: 20170626 Address after: 100027 Beijing City, Dongcheng District No. 40 East Street, Yuanjia Residence Internazionale B block 3 layer Patentee after: TransEasy Medical Tech. Co.,Ltd. Address before: 214192, Jiangsu, Xishan Economic Development Zone, Wuxi science and Technology Park (Furong Road three, 99), Qingyun six Patentee before: Wuxi Zhongke Guangyuan Biomaterial Co., Ltd. |
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