WO2007007041A1 - Heteroaryl benzamide derivatives for use as glk activators in the treatment of diabetes - Google Patents

Heteroaryl benzamide derivatives for use as glk activators in the treatment of diabetes Download PDF

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Publication number
WO2007007041A1
WO2007007041A1 PCT/GB2006/002471 GB2006002471W WO2007007041A1 WO 2007007041 A1 WO2007007041 A1 WO 2007007041A1 GB 2006002471 W GB2006002471 W GB 2006002471W WO 2007007041 A1 WO2007007041 A1 WO 2007007041A1
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Prior art keywords
oxy
methyl
ylcarbonyl
azetidin
benzamide
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PCT/GB2006/002471
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French (fr)
Inventor
Darren Mckerrecher
Kurt Gordon Pike
Michael James Waring
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Astrazeneca Ab
Astrazeneca Uk Limited
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Priority claimed from GB0514173A external-priority patent/GB0514173D0/en
Priority claimed from GB0516297A external-priority patent/GB0516297D0/en
Priority claimed from GB0523862A external-priority patent/GB0523862D0/en
Priority claimed from GB0524589A external-priority patent/GB0524589D0/en
Priority claimed from GB0607977A external-priority patent/GB0607977D0/en
Priority to MX2008000172A priority Critical patent/MX2008000172A/en
Priority to EP06755703A priority patent/EP1902052B1/en
Priority to US11/995,077 priority patent/US7642259B2/en
Priority to NZ564608A priority patent/NZ564608A/en
Application filed by Astrazeneca Ab, Astrazeneca Uk Limited filed Critical Astrazeneca Ab
Priority to ES06755703T priority patent/ES2397648T3/en
Priority to CA2614746A priority patent/CA2614746C/en
Priority to AU2006268406A priority patent/AU2006268406C1/en
Priority to KR1020087002533A priority patent/KR101346902B1/en
Priority to BRPI0613570A priority patent/BRPI0613570B8/en
Priority to JP2008520936A priority patent/JP4651714B2/en
Publication of WO2007007041A1 publication Critical patent/WO2007007041A1/en
Priority to IL188059A priority patent/IL188059A0/en
Priority to NO20076658A priority patent/NO20076658L/en
Priority to HK08106758.1A priority patent/HK1116769A1/en
Priority to US12/202,800 priority patent/US7642263B2/en
Priority to US12/203,324 priority patent/US7842694B2/en
Priority to US12/256,254 priority patent/US7977328B2/en
Priority to AU2010235903A priority patent/AU2010235903B2/en
Priority to IL215102A priority patent/IL215102A0/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
    • C07D417/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing three or more hetero rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/397—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having four-membered rings, e.g. azetidine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/4427—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/04—Anorexiants; Antiobesity agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/14—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
    • C07D403/14—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/14—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D413/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/08—Bridged systems

Definitions

  • the present invention relates to a group of benzoyl amino heterocyclyl compounds which are useful in the treatment or prevention of a disease or medical condition mediated through glucokinase (GLK or GK), leading to a decreased glucose threshold for insulin secretion.
  • GLK or GK glucokinase
  • the compounds are predicted to lower blood glucose by increasing hepatic glucose uptake.
  • Such compounds may have utility in the treatment of Type 2 diabetes and obesity.
  • the invention also relates to pharmaceutical compositions comprising said compounds and to methods of treatment of diseases mediated by GLK using said compounds. ' /
  • the main plasma membrane glucose transporter is GLUT2.
  • G-6-P glucose-6-phosphate
  • GLK glucokinase
  • GLK has a high (6-1OmM) Km for glucose and is not inhibited by physiological concentrations of G-6-P [I].
  • GLK expression is limited to a few tissues and cell types, most notably pancreatic ⁇ -cells and liver cells (hepatocytes) [I].
  • GLK activity is rate limiting for glucose utilisation and therefore regulates the extent of glucose induced insulin secretion and hepatic glycogen synthesis. These processes are critical in the maintenance of whole body glucose homeostasis and both are dysfunctional in diabetes [2].
  • Maturity-Onset Diabetes of the Young Type 2 the diabetes is caused by GLK loss of function mutations [3, 4].
  • Hyperglycaemia in MODY-2 patients results from defective glucose utilisation in both the pancreas and liver [5].
  • Defective glucose utilisation in the pancreas of MODY-2 patients results in a raised threshold for glucose stimulatedii ⁇ sulin secretion.
  • rare activating mutations of GLK reduce this threshold resulting in familial hyperinsulinism [6, 6a, 7].
  • hepatic glucokinase activity is also decreased in type 2 diabetics [8].
  • GLK global or liver selective overexpression of GLK prevents or reverses the development of the diabetic phenotype in both dietary and genetic models of the disease [9-12].
  • acute treatment of type 2 diabetics with fructose improves glucose tolerance through stimulation of hepatic glucose utilisation [13]. This effect is believed to be mediated through a fructose induced increase in cytosolic GLK activity in the hepatocyte by the mechanism described below [13].
  • Hepatic GLK activity is inhibited through association with GLK regulatory protein
  • the GLK/GLKRP complex is stabilised by fructose-6-phosphate (F6P) binding to the GLKRP and destabilised by displacement of this sugar phosphate by fructose- 1 -phosphate (FlP).
  • FlP is generated by fructokinase mediated phosphorylation of dietary fructose. Consequently, GLK/GLKRP complex integrity and hepatic GLK activity is regulated in a nutritionally dependent manner as F6P is dominant in the post-absorptive state whereas FlP predominates in the post-prandial state.
  • the pancreatic ⁇ -cell expresses GLK in the absence of GLKRP.
  • ⁇ -cell GLK activity is regulated extensively by the availability of its substrate, glucose.
  • Small molecules may activate GLK either directly or through destabilising the GLK/GLKRP complex.
  • the former class of compounds are predicted to stimulate glucose utilisation in both the liver and the pancreas whereas the latter are predicted to act selectively in the liver.
  • compounds with either profile are predicted to be of therapeutic benefit in treating Type 2 diabetes as this disease is characterised by defective glucose utilisation in both tissues.
  • GLK, GLKRP and the K ATP channel are expressed in neurones of the hypothalamus, a region of the brain that is important in the regulation of energy balance and the control of food intake [14-18].
  • GLK activators may be of therapeutic use in treating eating disorders, including obesity, in addition to diabetes.
  • the hypothalamic effects will be additive or synergistic to the effects of the same compounds acting in the liver and/or pancreas in normalising glucose homeostasis, for the treatment of Type 2 diabetes.
  • the GLK/GLKRP system can be described as a potential "Diabesity" target (of benefit in both Diabetes and Obesity).
  • GLK is also expressed in specific entero-endocrine cells where it is believed to control the glucose sensitive secretion of the incretin peptides GIP (glucose-dependent insulinotropic polypeptide) and GLP-I (Glucagon-Like Peptide- 1) from gut K-cells and L- cells respectively (32, 33, 34). Therefore, small molecule activators of GLK may have additional beneficial effects on insulin secretion, b-cell function and survival and body weight as a consequence of stimulating GIP and GLP-I secretion from these entero- endocrine cells.
  • GIP glucose sensitive secretion of the incretin peptides
  • GLP-I Glucagon-Like Peptide- 1
  • glucokinase activators In WO00/58293 and WO01/44216 (Roche), a series of benzylcarbamoyl compounds are described as glucokinase activators. The mechanism by which such compounds activate GLK is assessed by measuring the direct effect of such compounds in an assay in which GLK activity is linked to NADH production, which in turn is measured optically - see details of the in vitro assay described hereinafter.
  • Compounds of the present invention may activate GLK directly or may activate GLK by inhibiting the interaction of GLKRP with GLK.
  • GLK activators have been described in WO03/095438 (substituted phenylacetamides, Roche), WO03/055482 (carboxamide and sulphonamide derivatives, Novo Nordisk), WO2004/002481 (arylcarbonyl derivatives, Novo Nordisk), and in WO03/080585 (amino-substituted benzoylaminoheterocycles, Banyu).
  • WO03/000267 describes a group of benzoyl amino pyridyl carboxylic acids which are activators of the enzyme glucokinase (GLK).
  • WO03/015774 describes compounds of the
  • R 3 is a substituted heterocycle other than a carboxylic acid substituted pyridyl.
  • International application WO2004/076420 (Banyu) describes compounds which are generally a subset of those described in WO03/015774, wherein for example R 1 is an (substituted) alkyl ether and R 2 is (substituted) phenoxy.
  • the compounds of the invention may also have superior potency and/or advantageous physical properties (as described above) and/or favourable toxicity profiles and/or favourable metabolic profiles in comparison with other GLK activators known in the art, as well as those described in WO 03/015774.
  • HET-2 is a 5- or 6- membered heteroaryl ring, containing 1, 2 or 3 ring hetereoatoms independently selected from O, S and N; which ring is substituted on an available carbon atom by a substituent selected from R 2 , and is optionally further substituted on 1 or 2 available carbon atoms by a substituent independently selected from R 3 and/or on an available nitrogen atom (provided it is not thereby quaternised) by a substituent selected
  • R 2 is selected from -C(O)NR 4 R 5 and -SO 2 NR 4 R 5 ;
  • R 3 is selected from methyl, trifluoromethyl and halo;
  • R 4 and R 5 together with the nitrogen atom to which they are attached form a 4 to 7 membered saturated or partially unsaturated heterocyclyl ring, optionally containing 1 or 2 further heteroatoms (in addition to the linking N atom) independently selected from O, N and S, wherein a -CH 2 - group can optionally be replaced by a -C(O)- and wherein a sulphur atom in the ring may optionally be oxidised to a S(O) or S(O) 2 group; which ring is optionally substituted on an available carbon atom by 1 or 2 substituents independently selected from R 8 and/or on an available nitrogen atom by a substituent selected from R 9 ; or R 4 and R 5 together with the nitrogen atom to which they are attached form a 6-10 membered bicyclic
  • R 6 is independently selected from (l-4C)alkyl, halo, hydroxy(l-4C)alkyl, (l-4C)alkoxy(l- 4C)alkyl, (l-4C)alkylS(O)p(l-4C)alkyl, amino(l-4C)alkyl, (l-4C)alkylamino(l-4C)alkyl and di(l-4C)alkylamino(l-4C)alkyl;
  • R 7 is independently selected from (l-4C)alkyl, halo(l-4C)alkyl, dihalo(l-4C)alkyl, trihalo(l-4C)alkyl, hydroxy(l-4C)alkyl, (l-4C)alkoxy(l-4C)alkyl, (l-4C)alkylS(0)p(l- 4C)alkyl, amino(l-4C)alkyl, (l-4C)alkylamino(l-4C)alkyl and di(l-4
  • R 8 is selected from hydroxy, (l-4C)alkoxy, (l-4C)alkyl, aminocarbonyl, (1-
  • R 9 is selected from (l-4C)alkyl, -C(O)(I -4C)alkyl, aminocarbonyl, (1-
  • R 10 is selected from (l-4C)alkyl, (3-6C)cycloalkyl, hydroxy(l-4C)alkyl, (l-4C)alkoxy(l- 4C)alkyl, -C(O)(I -4C)alkyl, benzyl, and (l-4C)alkylsulfonyl; p is (independently at each occurrence) O, 1 or 2; or a salt thereof.
  • R 1 is selected from isopropyl, but-2-yl, 1,1,1-trifluoroprop- 2-yl, l,3-difluoroprop-2-yl, but-l-yn-3-yl, l-hydroxyprop-2-yl, 2-hydroxybut-3-yl, 1- hydroxybut-2-yl, tetrahydrofuryl, tetrahydropyranyl, l-methoxyprop-2-yl, 1-methoxybut-
  • HET-I and HET-2 encompass heteroaryl rings which may be substituted on nitrogen, such substitution may not result in charged quaternary nitrogen atoms, removal of aromaticity of the ring or unstable structures. It will be appreciated that the definitions of HET-I and HET-2 are not intended to include any O-O, O-S or S-S bonds. It will be appreciated that the definitions of HET-I and HET-2 are not intended to include unstable structures.
  • any single carbon atom in HET-I may only be substituted by one group R 6 in order to maintain aromaticity of the ring.
  • Up to two different carbon atoms in a HET-I ring may be substituted by an R 6 group, each of which may be the same or different, provided the structure thereby formed is stable and aromatic.
  • any single carbon atom in HET-2 may only be substituted by one group R 3 in order to maintain aromaticity of the ring.
  • Up to two different carbon atoms in a HET-2 ring may be substituted by an R 3 group, each of which may be the same or different, provided the structure thereby formed is stable and aromatic.
  • R 8 can be present on any or all available carbon atoms in the heterocyclic ring formed by NR 4 R 5 ; each carbon atom can be substituted with 1 or 2 R 8 groups which may be the same or different, provided the structure thereby formed is stable (so, for example, it is not intended to cover gem-dihydroxy substitution).
  • the invention relates to compounds of formula (I) as hereinabove defined or to a pharmaceutically acceptable salt.
  • alkyl includes both straight-chain and branched-chain alkyl groups. However references to individual alkyl groups such as “propyl” are specific for the straight chain version only and references to individual branched-chain alkyl groups such as t-butyl are specific for the branched chain version only.
  • (l-4C)alkyl includes methyl, ethyl, propyl, isopropyl and t-butyl. An analogous convention applies to other generic terms.
  • HET-I containing a nitrogen in the 2-position
  • HET-I encompasses but is not limited to the following structures:
  • HET-I as a 5- or 6-membered, C-linked heteroaryl ring as hereinbefore defined, include thiazolyl, isothiazolyl, thiadiazolyl, pyridyl, pyrazinyl, pyridazinyl, pyrazolyl, imidazolyl, pyrimidinyl, oxazolyl, isoxazolyl, oxadiazolyl and triazolyl.
  • HET-2 examples include thienyl, furyl, thiazolyl, isothiazolyl, thiadiazolyl, pyridyl, pyrazinyl, pyridazinyl, pyrazolyl, imidazolyl, pyrimidinyl, oxazolyl, isoxazolyl, oxadiazolyl and triazolyl.
  • Further suitable examples of HET-2 include aromatic heterocycles where a ring nitrogen or sulfur atom has been oxidised but aromaticity has been preserved, for example a pyridine N-oxide.
  • HET-2 include thiazolyl, pyridyl, pyrazinyl, pyridazinyl and pyrimidinyl.
  • Suitable examples for a 4-7 membered ring formed by R 4 and R 5 together with the nitrogen to which they are attached, as hereinbefore defined, include morpholino, piperidinyl, piperazinyl, pyrrolidinyl, azetidinyl, homopiperazinyl, homo-morpholino, homo-thiomorpholino (and versions thereof wherein the sulfur is oxidised to an SO or S(O) 2 group) and homo-piperidinyl.
  • a further suitable example is thiomorpholino (and versions thereof wherein the sulfur is oxidised to an SO or S(O) 2 group).
  • Suitable examples for a 6-10 membered bicyclic heterocyclic ring formed by R 4 and R 5 together with the nitrogen to which they are attached, as hereinbefore defined, are bicyclic saturated or partially unsaturated heterocyclyl ring such as those illustrated by the structures shown below (wherein the dotted line indicates the point of attachment to the rest of the molecule and wherein R represents the optional substituents for carbon or nitrogen defined hereinbefore):
  • a ring system is a [2,2,1] system such as
  • such a ring system is a [2.1.1] system such as
  • Examples of (l-4C)alkyI include methyl, ethyl, propyl, isopropyl, butyl and tert- butyl; examples of (3-6C)cycloalkyl include cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl; examples of halo include fluoro, chloro, bromo and iodo; examples of halo(l- 4C)aIkyl include fluoromethyl, chloromethyl, fluoroethyl, chloroethyl, fluoropropyl and fluorobutyl; examples of dihalo(l-4C)alkyl include difluoromethyl, l,l-difluoroeth-2-yl, l,2-difluoroeth-2-yl, l,l-dichloroeth-2-yl, l,2-dichloroeth-2-yl, and l,l-difluor
  • the invention includes in its definition any such optically active or racemic form which possesses the property of stimulating GLK directly or inhibiting the GLK/GLKRP interaction.
  • the synthesis of optically active forms may be carried out by standard techniques of organic chemistry well known in the art, for example by synthesis from optically active starting materials or by resolution of a racemic form.
  • certain compounds may exist in tautomeric forms and that the invention also relates to any and all tautomeric forms of the compounds of the invention which activate GLK.
  • the invention relates to compounds of formula (I) as hereinabove defined or to a pro-drug thereof.
  • Suitable examples of pro-drugs of compounds of formula (I) are in- vivo hydrolysable esters of compounds of formula (I). Therefore in another aspect, the invention relates to compounds of formula (I) as hereinabove defined or to an in- vivo hydrolysable ester thereof.
  • compounds of formula (I) in an alternative embodiment are provided pharmaceutically-acceptable salts of compounds of formula (I), in a further alternative embodiment are provided in-vivo hydrolysable esters of compounds of formula (I), and in a further alternative embodiment are provided pharmaceutically-acceptable salts of in-vivo hydrolysable esters of compounds of formula (I).
  • the compounds of the invention may be administered in the form of a pro-drug.
  • a pro-drug is a bioprecursor or pharmaceutically acceptable compound being degradable in the body to produce a compound of the invention (such as an ester or amide of a compound of the invention, particularly an in-vivo hydrolysable ester).
  • Various forms of prodrugs are known in the art. For examples of such prodrug derivatives, see: a) Design of Prodrugs, edited by H. Bundgaard, (Elsevier, 1985) and Methods in En ⁇ ymology, Vol. 42, p. 309-396, edited by K. Widder, et al. (Academic Press, 1985); b) A Textbook of Drug Design and Development, edited by Krogsgaard-Larsen; c) H. Bundgaard, Chapter 5 "Design and Application of Prodrugs", by H.
  • Suitable pharmaceutically-acceptable esters for carboxy include Ci to C ⁇ alkoxymethyl esters for example methoxymethyl, C 1 to C ⁇ alkanoyloxymethyl esters for example pivaloyloxymethyl, phthalidyl esters, C 3 to Cscycloalkoxycarbonyloxyd to C 6 alkyl esters for example l-cyclohexylcarbonyloxyethyl; l,3-dioxolen-2-onylmethyl esters, for example 5-methyl-l,3-dioxolen-2-onylmethyl; and (l-6C)alkoxycarbonyloxyethyl esters.
  • An in-vivo hydrolysable ester of a compound of the invention containing a hydroxy group includes inorganic esters such as phosphate esters (including phosphoramidic cyclic esters) and ⁇ -acyloxyalkyl ethers and related compounds which as a result of the in- vivo hydrolysis of the ester breakdown to give the parent hydroxy group/s.
  • inorganic esters such as phosphate esters (including phosphoramidic cyclic esters) and ⁇ -acyloxyalkyl ethers and related compounds which as a result of the in- vivo hydrolysis of the ester breakdown to give the parent hydroxy group/s.
  • ⁇ -acyloxyalkyl ethers include acetoxymethoxy and 2,2-dimethylpropionyloxy-methoxy.
  • a selection of in- vivo hydrolysable ester forming groups for hydroxy include alkanoyl, benzoyl, phenylacetyl and substituted benzoyl and phenylacetyl, alkoxycarbonyl (to give alkyl carbonate esters), dialkylcarbamoyl and N- (dialkylaminoethyl)-N-alkylcarbamoyl (to give carbamates), dialkylaminoacetyl and carboxyacetyl.
  • a suitable pharmaceutically-acceptable salt of a compound of the invention is, for example, an acid-addition salt of a compound of the invention which is sufficiently basic, for example, an acid-addition salt with, for example, an inorganic or organic acid, for example hydrochloric, hydrobromic, sulphuric, phosphoric, trifluoroacetic, citric or maleic acid.
  • a suitable pharmaceutically-acceptable salt of a benzoxazinone derivative of the invention which is sufficiently acidic is an alkali metal salt, for example a sodium or potassium salt, an alkaline earth metal salt, for example a calcium or magnesium salt, an ammonium salt or a salt with an organic base which affords a physiologically-acceptable cation, for example a salt with methylamine, dimethylamine, trimethylamine, piperidine, morpholine or tris-(2-hydroxyethyl)amine.
  • each of X 1 , X 2 and X 3 is independently selected from CH, N, S and O;
  • X 4 is absent (to make a 5-membered ring) or is selected from CH, N, O and S; provided that at least one of X 1 , X 2 , X 3 and X 4 is CH and provided that there are no O-O, O-S or S-S bonds within the ring;
  • R 3 if present, is selected from methyl, trifluoromethyl and halo;
  • R 1 , R 2 and HET-I are as defined for a compound of formula (I). It will be understood that the dotted circle inside the ring containing X 1 to X 4 (that is, the HET-2 ring) is intended to indicate that the ring is aromatic, although the precise number and position of the double bonds will be dependent on the nature of X 1 to X 4 .
  • Particular examples of compounds of formula (I) and (IA) include compounds of formulae (IB), (IC) and/or (ID):
  • R 1 , R 2 and HET-I are as defined for a compound of formula (I).
  • Preferred values of each variable group are as follows. Such values may be used where appropriate with any of the values, definitions, claims, aspects or embodiments defined hereinbefore or hereinafter. In particular, each may be used as an individual limitation on the broadest definition of formula (I) or (IA). Further, each of the following values may be used in combination with one or more of the other following values to limit the broadest defintion of formula (I) or (IA) or to limit any narrower definitions of formula (I) or (IA) in any of the aspects hereinbefore or hereinafter. Where appropriate each of the following values may also be used to limit any definition within formulae (IB), (IC) and/or (ID). (1) R 1 is of sub-formula X:
  • R x is selected from methyl, ethyl, trifluoromethyl, ethynyl, hydroxymethyl, hydroxyethyl, methoxymethyl, fluoromethoxymethyl, difluoromethoxymethyl and trifluoromethoxymethyl; preferably R x is selected from methyl, ethyl, trifluoromethyl, ethynyl, hydroxymethyl, hydroxyethyl, methoxymethyl, fluoromethoxymethyl and difluoromethoxymethyl
  • R 1 is of sub-formula Y:
  • R 1 is l-hydroxyprop-2-yl and the configuration is preferably (S), that is R ! -O- is:
  • R 1 is l-methoxyprop-2-yl and the configuration is preferably (S), that is R ⁇ O- is:
  • R 1 is selected from isopropyl, l,3-difluoroprop-2-yl, but-l-yn-3-yl, l-hydroxyprop-2- yl, hydroxybut-3-yl and l-methoxyprop-2-yl
  • R 1 is l,l,l-trifluoroprop-2-yl, l-f ⁇ uoromethoxyprop-2-yl, l,l-difluoromethoxyprop-2- yl or l-trifluoromethoxyprop-2-yl
  • R 1 is l-fluoromethoxyprop-2-yl, l,l-difluoromethoxyprop-2-yl or 1- trifluoromethoxyprop-2-yl, particularly l-fluoromethoxyprop-2-yl or 1,1- difluoromethoxyprop-2-yl
  • R 1 is l,l-difluoromethoxyprop-2-yl, particularly with the stereochemistry:
  • R 1 is tetrahydrofuryl or tetrahydropyranyl
  • R 1 is tetrahydrofuryl in the (S) configuration, that is:
  • R 1 is tetrahydrofuryl in the (R) configuration, that is:
  • R 1 is 4-tetrahydropyranyl
  • R 1 is 2-hydroxy-but-3-yl and the configuration is preferably such that R J -O- is:
  • R 1 is l-hydroxybut-2-yl or l-methoxybut-2-yl
  • R 1 is selected from isopropyl, but-2-yl, l,l,l-trifluoroprop-2-yl, l,3-difluoroprop-2- yl, but-l-yn-3-yl, l-hydroxyprop-2-yl, 2-hydroxybut-3-yl, tetrahydrofuryl, tetrahydropyranyl, l-methoxyprop-2-yl, l-fluoromethoxyprop-2-yl, 1,1- difluoromethoxyprop-2-yl and l-trifluoromethoxyprop-2-yl
  • R 1 is selected from 2-hydroxyprop-l-yl, 2-methoxyprop-l-yl, 2-hydroxybut-l-yl and 2-methoxybut- 1 -yl
  • R 1 is selected from isopropyl, l,3-difluoroprop-2-yl, but-2-yl, l-hydroxyprop-2-yl, 2- hydroxybut-3-yl, 1,1 -difluoromethoxyprop-2-yl, tetrahydrofuryl, l-hydroxybut-2-yl and 1- methoxyprop-2-yl (18)
  • R 1 is selected from isopropyl, cyclopentyl, l,3-difluoroprop-2-yl, but-2-yl, 1- hydroxyprop-2-yl, 2-hydroxybut-3-yl, l,l-difluoromethoxyprop-2-yl, tetxahydrofuryl, tetrahydropyranyl, l-hydroxybut-2-yl and l-methoxyprop-2-yl
  • R 1 is selected from tetrahydrofuryl, l-difluoromethoxyprop-2-yl, l,3-difluoroprop-2- yl, and 2-hydroxybut-3-yl
  • R 1 is selected from isopropyl, tetrahydrofuryl, l-hydroxyprop-2-yl and 1- methoxyprop-2-yl
  • R 1 is selected from l-hydroxyprop-2-yl and l-methoxyprop-2-yl, for example (2S)- 1- hydroxyprop-2-yl and (25)- l-methoxyprop-2-yl
  • R 1 is selected from isopropyl, but-2-yl, cyclopentyl, l,l,l-trifluoroprop-2-yl, 1,3- difluoroprop-2-yl, but-l-yn-3-yl, l-hydroxyprop-2-yl, 2-hydroxybut-3-yl, l-hydroxybut-2- yl, tetrahydrofuryl, tetrahydropyranyl, l-methoxyprop-2-yl, l-methoxybut-2-yl, 2- hydroxyprop-1-yl, 2-methoxyprop-l-yl, 2-hydroxybut-l-yl, 2-methoxybut-yl-yl, 1- fluoromethoxyprop-2-yl and 1, l-di
  • HET-I is a 6-membered heteroaryl ring
  • HET-I is substituted with 1 or 2 substituents independently selected from R 6
  • HET-I is substituted with 1 substituent selected from R 6
  • HET-I is substituted with 1 substituent selected from R 7 (29) HET-I is unsubstituted
  • HET-I is selected from thiazolyl, isothiazolyl, thiadiazolyl, pyridyl, pyrazinyl, pyridazinyl, pyrazolyl, imidazolyl, pyrimidinyl, oxazolyl, isoxazolyl, oxadiazolyl, and triazolyl
  • HET-I is selected from thiazolyl, isothiazolyl, thiadiazolyl, pyrazolyl, imidazolyl, oxazolyl, isoxazolyl and oxadiazolyl
  • HET-I is selected from pyridyl, pyrazinyl, pyridazinyl and pyrimidinyl
  • HET-I is selected from thiazolyl, pyrazolyl and oxazolyl (34) HET-I is selected from thiadiazolyl and oxadiazolyl
  • HET-I is selected from 1,3,4-thiadiazolyl and 1,3,4-oxadiazolyl
  • HET-I is selected from 1,2,4-oxadiazolyl and 1,2,4-oxadiazolyl
  • HET-I is pyrazolyl, particularly N-methylpyrazolyl (38) HET-I is pyrazinyl
  • HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl and pyrazinyl
  • HET-I is selected from thiazolyl, pyrazolyl, and thiadiazolyl, optionally substituted with (l-4C)alkyl
  • HET-I is pyrazolyl, particularly N-methylpyrazolyl (42) HET-I is pyrazolyl (optionally substituted with ethyl, isopropyl or 1 or 2 methyl), thiazolyl (optionally substituted with methyl), pyrazinyl (optionally substituted with methyl), pyridyl (optionally substituted by fluoro), isoxazolyl (optionally substituted with methyl) and thiadiazolyl (optionally substituted with methyl)
  • HET-I is pyrazolyl (optionally substituted with ethyl, isopropyl, difluoromethyl, or 1 or 2 methyl), thiazolyl (optionally substituted with methyl), pyrazinyl (optionally substituted with methyl), pyridyl (optionally substituted by fluoro), isoxazolyl (optionally substituted with methyl) and thiadiazolyl (optionally substituted with methyl)
  • HET-I is selected from pyrazinyl (optionally substituted with methyl), pyrazolyl (optionally substituted on carbon by methyl), methylthiadiazolyl (particularly 1,2,4- thiadiazol-5-yl, more particularly 3-methyl- 1 ,2,4-thiadiazol-5-yl), thiazolyl (optionally substituted with methyl), pyridyl (optionally substituted by fluoro) and isoxazolyl
  • HET-I is N-difluoromethylpyrazolyl
  • HET-I is 5-methylpyrazin-2-yl
  • HET-I is pyrazolyl (optionally substituted with ethyl, isopropyl, difluoromethyl, or 1 or 2 methyl), thiazolyl (optionally substituted with methyl), pyrazinyl (optionally substituted with methyl), pyridyl (optionally substituted by fluoro), isoxazolyl (optionally substituted with methyl) and thiadiazolyl (optionally substituted with methyl); and R 1 is selected from isopropyl, cyclopentyl, l,3-difluoropro ⁇ -2-yl, but-2-yl, 1- hydroxyprop-2-yl, 2-hydroxybut-3-yl, l,l-difluoromethoxyprop-2-yl, tetrahydroruryl, tetrahydropyranyl, l-hydroxybut-2-yl and l-methoxy ⁇ rop-2-yl; when HET-I is pyrazolyl unsubstituted
  • HET-I is methylpyrazinyl and R 1 is selected from l-hydroxyprop-2-yl and 1- methoxyprop-2-yl, for example (2S)- l-hydroxyprop-2-yl and (2S)- l-methoxyprop-2-yl
  • HET-I is pyrazolyl and R 1 is selected from isopropyl, tetrahydrofuryl and 1- methoxyprop-2-yl, for example (2S)- l-methoxyprop-2-yl
  • R 6 is selected from (l-4C)alkyl, halo, hydroxy(l-4C)alkyl and di(l-4C)alkylamino(l- 4C)alkyl
  • R 6 is selected from methyl, ethyl, bromo, chloro, fluoro, hydroxymethyl, methoxymethyl, aminomethyl, N-methylaminomethyl, dimethylaminomethyl
  • R 6 is selected from (l-4C)alkyl, halo, hydroxy(l-4C)alkyl, (l-4C)alkoxy(l-4C)alkyl, (l-4C)alkylS(O)p(l-4C)alkyl, amino(l-4C)alkyl, (l-4C)alkylamino(l-4C)alkyl, and di(l- 4C)alkylamino( 1 -4C)alkyl
  • R 6 is selected from methyl, ethyl, bromo, chloro, fluoro, hydroxymethyl,
  • R 6 is selected from methyl, ethyl, chloro and fluoro
  • R 6 is methyl or fluoro, preferably methyl
  • R 6 is selected from methyl, ethyl, bromo, chloro, fluoro, aminomethyl, N- methylaminomethyl, dimethylaminomethyl, hydroxymethyl and methoxymethyl
  • R 6 is selected from methyl, ethyl, aminomethyl, N-methylaminomethyl, dimethylaminomethyl, hydroxymethyl and methoxymethyl
  • R 6 is selected from methyl, ethyl, isopropyl and methoxymethyl
  • R 6 is selected from (l-4C)alkylS(O)p(l-4C)alkyl, (l-4C)alkylamino(l-4C)alkyl and di(l-4C)alkylamino(l-4C)alkyl
  • R 7 is selected from (l-4C)alkyl, hydroxy(l-4C)alkyl and di(l-4C)alkylamino(l- 4C)alkyl
  • R 7 is selected from methyl, ethyl, hydroxymethyl, methoxymethyl, aminomethyl, N-methylaminomethyl, dimethylaminomethyl (64)
  • R 7 is selected from (l-4C)alkyl, hydroxy(l-4C)alkyl, (l-4C)alkoxy(l-4C)alkyl, (1- 4C)alkylS(O)p(l-4C)alkyl, amino(l-4C)alkyl, (l-4C)alkylamino(l-4C)alkyl, and di(l- 4C)alkylamino(l-4C)alkyl
  • R 7 is selected from methyl, ethyl, aminomethyl, N-methylaminomethyl, and dimethylaminomethyl (66) R 7 is selected from methyl, ethyl, hydroxymethyl and methoxymethyl
  • R 7 is selected from methyl, isopropyl and ethyl
  • R 7 is selected from methyl, isopropyl, difluoromethyl and ethyl
  • R 7 is selected from isopropyl and difluoromethyl, particularly difluoromethyl
  • R 7 is selected from methyl and ethyl (71) R 7 is methyl
  • R 7 is selected from methyl, ethyl, aminomethyl, N-methylaminomethyl, dimethylaminomethyl, hydroxymethyl and methoxymethyl
  • R 7 is selected from methyl, ethyl, isopropyl and methoxymethyl
  • R 7 when R 7 is selected from halo(l-4C)alkyl, dihalo(l-4C)alkyl and trihalo(l-4C)alkyl, each halo is selected from chloro and fluoro, and is in particular fluoro.
  • R 7 when R 7 is selected from halo(l-4C)alkyl, dihalo(l-4C)alkyl and trihalo(l-4C)alkyl, R 7 is particularly selected from fluoromethyl, difluoroethyl, difluoromethyl and trifluoromethyl
  • HET-2 is a 5-membered ring
  • HET-2 is a 6-membered ring
  • HET-2 is selected from thienyl, furyl, thiazolyl, isothiazolyl, thiadiazolyl, pyridyl, pyrazinyl, pyridazinyl, pyrazolyl, imidazolyl, pyrimidinyl, oxazolyl, isoxazolyl and oxadiazolyl
  • HET-2 is selected from thienyl, furyl, thiadiazolyl, pyridyl, pyrazinyl, pyridazinyl, pyrazolyl, imidazolyl, pyrimidinyl and oxadiazolyl
  • HET-2 is selected from pyridyl, pyrazinyl, thiazolyl and thienyl
  • HET-2 is selected from pyridyl, pyrazinyl and thiazolyl
  • HET-2 is selected from pyridyl, pyrazinyl, pyridazinyl and thiazolyl
  • HET-2 is selected from pyridyl and pyrazinyl
  • HET-2 is pyrazinyl
  • HET-2 is substituted with a substituent selected from R 3 (86) HET-2 has one nitrogen substituent selected from R 10
  • R 3 is chloro or fluoro
  • R 3 is chloro or methyl (91) R 3 is fluoro, chloro or methyl
  • R 2 is -C(O)NR 4 R 5
  • R 4 and R 5 together with the nitrogen atom to which they are attached form a ring selected from morpholino, piperidinyl, piperazinyl, pyrrolidinyl and azetidinyl (100) R 4 and R 5 together with the nitrogen atom to which they are attached form a ring selected from pyrrolidinyl, morpholino and azetidinyl
  • R 4 and R 5 together with the nitrogen atom to which they are attached form a ring selected from 7-azabicyclo[2.2.1]hept-7-yl, pyrrolidinyl, morpholino and azetidinyl
  • R 4 and R 5 together with the nitrogen atom to which they are attached form an azetidinyl or pyrrolidinyl ring (104) R 4 and R 5 together with the nitrogen atom to which they are attached form an unsubstituted ring
  • R 4 and R 5 together with the nitrogen atom to which they are attached form an ring mono-substituted either with a substituent R 8 or with a substituent R 9 (106) R 4 and R 5 together with the nitrogen atom to which they are attached form a 6-10 membered bicyclic saturated or partially unsaturated ring
  • R 8 is selected from hydroxy, (l-4C)alkoxy, (l-4C)alkyl
  • R 8 is selected from hydroxy, methoxy and methyl
  • R 9 is selected from (l-4C)alkyl and -C(O)(I -4C)alkyl (110)
  • R 2 is azetidinylcarbonyl or pyrrolidinylcarbonyl, preferably azetidinylcarbonyl (109)
  • R 10 is (l-4C)alkyl
  • R 10 is (3-6C)cycloalkyl
  • R 10 is hydroxy(l-4C)alkyl or (l-4C)alkoxy(l-4C)alkyl
  • R 10 is -C(O)(l-4C)alkyl
  • R 10 is benzyl
  • R 10 is (l-4C)alkylsulfonyl
  • R 10 is (l-4C)alkyl or benzyl
  • R 1 is selected from isopropyl, but-2-yl, l,l,l-trifluoroprop-2-yl, l,3-difluoroprop-2-yl, but-l-yn-3-yl, l-hydroxyprop-2-yl, 2-hydroxybut-3-yl, tetrahydrofuryl, tetrahydropyranyl, l-methoxyprop-2-yl, l-fluoromethoxyprop-2-yl and l,l-difluoromethoxyprop-2-yl;
  • HET-I is a 5- or 6-membered, C-linked heteroaryl ring containing a nitrogen atom in the 2- position and optionally 1 or 2 further ring heteroatoms independently selected from O, N and S; which ring is optionally substituted on any nitrogen atom (provided it is not thereby quaternised) by a methyl or ethyl group and/or on 1 or 2 available carbon atoms by a methyl or ethyl group;
  • HET-2 is a 5- or 6- membered heteroaryl ring, containing 1, 2 or 3 ring hetereoatoms independently selected from O, S and N; which ring is substituted on an available carbon atom by a substituent selected from R 2 , and is optionally further substituted on 1 or 2 available carbon atoms by a substituent independently selected from R 3 and/or on an available nitrogen atom (provided it is not thereby quaternised) by a substituent selected from R 10 ;
  • R 2 is selected from -C(O)NR 4 R 5 and -SO 2 NR 4 R 5 ;
  • R 3 is selected from halo;
  • R 4 and R 5 together with the nitrogen atom to which they are attached form a 4 to 7 membered saturated or partially unsaturated heterocyclyl ring, optionally containing 1 or 2 further heteroatoms (in addition to the linking N atom) independently selected from O, N and S, wherein a -CH 2 - group can optionally be replaced by a -C(O)- and wherein a sulphur atom in the ring may optionally be oxidised to a S(O) or S(O) 2 group; which ring is optionally substituted on an available carbon atom by 1 or 2 substituents independently selected from R 8 and/or on an available nitrogen atom by a substituent selected from R 9 ;
  • R 8 is selected from hydroxy, (l-4C)alkoxy and (l-4C)alkyl;
  • R 9 is selected from (l-4C)alkyl, -C(O)(I -4C)alkyl, and -S(0)p(l-4C)alkyl;
  • R 10 is selected from (l-4C)alkyl, -C(O)(I -4C)alkyl, benzyl, and (l-4C)alkylsulfonyl; p is (independently at each occurrence) O, 1 or 2.
  • R 1 is selected from isopropyl, but-2-yl, l,l,l-trifluoroprop-2-yl, l,3-difluoroprop-2-yl, but-l-yn-3-yl, l-hydroxyprop-2-yl, 2-hydroxybut-3-yl, tetrahydrofuryl, tetrahydropyranyl, l-methoxy ⁇ rop-2-yl, 2-hydroxyprop-l-yl, 2-methoxyprop-l-yl, 2-hydroxybut-l-yl, 2- methoxybut-1-yl, l-fluoromethoxyprop-2-yl and l,l-difluoromethoxyprop-2-yl; HET-I is a 5- or 6-membered, C-linked heteroaryl ring containing a nitrogen atom in the 2- position and optionally
  • R 2 is selected from -C(O)NR 4 R 5 and -SO 2 NR 4 R 5 ;
  • R 3 is selected from halo;
  • R 4 and R 5 together with the nitrogen atom to which they are attached form a 4 to 7 membered saturated or partially unsaturated heterocyclyl ring, optionally containing 1 or 2 further heteroatoms (in addition to the linking N atom) independently selected from O, N and S, wherein a -CH 2 - group can optionally be replaced by a -C(O)- and wherein a sulphur atom in the ring may optionally be oxidised to a S(O) or S(O) 2 group; which ring is optionally substituted on an available carbon atom by 1 or 2 substituents independently selected from R 8 and/or on an available nitrogen atom by a substituent selected from R 9 ;
  • R 8 is selected from hydroxy, (l-4C)alkoxy and (l-4C)alkyl
  • R 9 is selected from (l-4C)alkyl, -C(O)(I -4C)alkyl, and-S(0)p(l-4C)alkyl;
  • R 10 is selected from (l-4C)alkyl, -C(O)(I -4C)alkyl, benzyl, and (l-4C)alkylsulfonyl; p is (independently at each occurrence) O, 1 or 2.
  • R 1 is selected from isopropyl, but-2-yl, cyclopentyl, l,l,l-trifluoroprop-2-yl, 1,3- difluoroprop-2-yl, but-l-yn-3-yl, l-hydroxyprop-2-yl, 2-hydroxybut-3-yl, tetrahydrofuryl, tetrahydropyranyl, l-methoxyprop-2-yl, 2-hydroxyprop-l-yl, 2-methoxyprop-l-yl, 2- hydroxybut-1-yl, 2-methoxybut-l-yl, l-fluoromethoxyprop-2-yl and 1,1- difluoromethoxyprop-2-yl;
  • HET-I is a 5- or 6-membered, C-linked heteroaryl ring containing a nitrogen atom in the 2- position and optionally 1 or 2 further ring heteroatoms independently selected from O, N and S; which ring is optionally substituted on any nitrogen atom (provided it is not thereby quaternised) by a methyl, ethyl or isopropyl group and/or on 1 or 2 available carbon atoms by a methyl, ethyl or fluoro group;
  • HET-2 is a 5- or 6- membered heteroaryl ring, containing 1, 2 or 3 ring hetereoatoms independently selected from O, S and N; which ring is substituted on an available carbon atom by a substituent selected from R 2 , and is optionally further substituted on 1 or 2 available carbon atoms by a substituent independently selected from R 3 and/or on an available nitrogen atom (provided it is not thereby quaternised) by a substituent selected
  • R 2 is selected from -C(O)NR 4 R 5 and -SO 2 NR 4 R 5 ;
  • R 3 is selected from halo;
  • R 4 and R 5 together with the nitrogen atom to which they are attached form a 4 to 7 membered saturated or partially unsaturated heterocyclyl ring, optionally containing 1 or 2 further heteroatoms (in addition to the linking N atom) independently selected from O, N and S, wherein a -CH 2 - group can optionally be replaced by a -C(O)- and wherein a sulphur atom in the ring may optionally be oxidised to a S(O) or S(O) 2 group; which ring is optionally substituted on an available carbon atom by 1 or 2 substituents independently selected from R 8 and/or on an available nitrogen atom by a substituent selected from R 9 ;
  • R 8 is selected from hydroxy, (l-4C)alkoxy and (l-4C)alkyl
  • R 9 is selected from (l-4C)alkyl, -C(O)(I -4C)alkyl, and -S(0)p(l-4C)alkyl;
  • R 10 is selected from (l-4C)alkyl, -C(O)(I -4C)alkyl, benzyl, and (l-4C)alkylsulfonyl; p is (independently at each occurrence) O, 1 or 2.
  • R 1 is selected from isopropyl, but-2-yl, cyclopentyl, l,l,l-trifluoroprop-2-yl, 1,3- difluoroprop-2-yl, but-l-yn-3-yl, l-hydroxyprop-2-yl, 2-hydroxybut-3-yl, tetrahydrofuryl, tetrahydropyranyl, l-methoxyprop-2-yl, 2-hydroxyprop-l-yl, 2-methoxyprop-l-yl, 2- hydroxybut-1-yl, 2-methoxybut-l-yl, l-fluoromethoxyprop-2-yl and 1,1- difluoromethoxyprop-2-yl;
  • HET-I is a 5- or 6-membered, C-linked heteroaryl ring containing a nitrogen atom in the 2- position and optionally 1 or 2 further ring heteroatoms independently selected from O, N and S; which ring is optionally substituted on any nitrogen atom (provided it is not thereby quaternised) by a methyl, ethyl or isopropyl group and/or on 1 or 2 available carbon atoms by a methyl, ethyl or fluoro group;
  • HET-2 is a 5 -or 6- membered heteroaryl ring, containing 1, 2 or 3 ring hetereoatoms independently selected from O, S and N; which ring is substituted on an available carbon atom by a substituent selected from R 2 , and is optionally further substituted on 1 or 2 available carbon atoms by a substituent independently selected from R 3 and/or on an available nitrogen atom (provided it is not thereby quaternised) by a substituent selected from R 10 ;
  • R 2 is selected from -C(O)NR 4 R 5 and -SO 2 NR 4 R 5 ;
  • R 3 is selected from methyl and halo;
  • R 4 and R 5 together with the nitrogen atom to which they are attached form a 4 to 7 membered saturated or partially unsaturated heterocyclyl ring, optionally containing 1 or 2 further heteroatoms (in addition to the linking N atom) independently selected from O, N and S, wherein a -CH 2 - group can optionally be replaced by a -C(O)- and wherein a sulphur atom in the ring may optionally be oxidised to a S(O) or S(O) 2 group; which ring is optionally substituted on an available carbon atom by 1 or 2 substituents independently selected from R 8 and/or on an available nitrogen atom by a substituent selected from R 9 ;
  • R 8 is selected from hydroxy, (l-4C)alkoxy and (l-4C)alkyl
  • R 9 is selected from (l-4C)alkyl, -C(O)(I -4C)alkyl, and-S(0)p(l-4C)alkyl;
  • R 10 is selected from (l-4C)alkyl, -C(O)(I -4C)alkyl, benzyl, and (l-4C)alkylsulfonyl; p is (independently at each occurrence) 0, 1 or 2.
  • R 1 is selected from isopropyl, but-2-yl, cyclopentyl, l,l,l-trifluoroprop-2-yl, 1,3- difluoroprop-2-yl, but-l-yn-3-yl, l-hydroxyprop-2-yl, 2-hydroxybut-3-yl, tetrahydrofuryl, tetrahydropyranyl, l-methoxyprop-2-yl, 2-hydroxyprop-l-yl, 2-methoxyprop-l-yl, 2- hydroxybut-1-yl, 2-methoxybut-l-yl, l-fluoromethoxyprop-2-yl and 1,1- difluoromethoxyprop-2-yl;
  • HET-I is a 5- or 6-membered, C-linked heteroaryl ring containing a nitrogen atom in the 2- position and optionally 1 or 2 further ring heteroatoms independently selected from O, N and S; which ring is optionally substituted on any nitrogen atom (provided it is not thereby quaternised) by a methyl, ethyl or isopropyl group and/or on 1 or 2 available carbon atoms by a methyl, ethyl or fluoro group;
  • HET-2 is a 5- or 6- membered heteroaryl ring, containing 1, 2 or 3 ring hetereoatoms independently selected from O, S and N; which ring is substituted on an available carbon atom by a substituent selected from R 2 , and is optionally further substituted on 1 or 2 available carbon atoms by a substituent independently selected from R 3 ;
  • R 2 is selected from -C(O)NR 4 R 5 and -SO 2 NR 4 R 5 ;
  • R 3 is selected from methyl and halo;
  • R 4 and R 5 together with the nitrogen atom to which they are attached form a 4 to 7 membered saturated or partially unsaturated heterocyclyl ring, optionally containing 1 or 2 further heteroatoms (in addition to the linking N atom) independently selected from O, N and S, wherein a -CH 2 - group can optionally be replaced by a -C(O)- and wherein a sulphur atom in the ring may optionally be oxidised to a S(O) or S(O) 2 group; or
  • R 4 and R 5 together with the nitrogen atom to which they are attached form a 6-10 membered bicyclic saturated or partially unsaturated heterocyclyl ring, optionally containing 1 further nitrogen atom (in addition to the linking N atom), wherein a -CH 2 - group can optionally be replaced by a -C(O)-.
  • R 1 is selected from isopropyl, but-2-yl, l,3-difluoroprop-2-yl, l-hydroxyprop-2-yl and 1- methoxyprop-2-yl and tetrahydrofuryl;
  • HET-I is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
  • HET-2 is a substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
  • R 2 is -CONR 4 R 5 ;
  • R 4 and R 5 together form an azetidinyl, pyrrolidinyl or morpholino ring.
  • R 1 is selected from isopropyl, l,3-difluoroprop-2-yl, but-2-yl, l-hydroxyprop-2-yl, 2- hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, l-hydroxybut-2-yl and 1- methoxyprop-2-yl;
  • HET-I is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
  • HET-2 is a substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
  • R 2 is -CONR 4 R 5 ;
  • R 4 and R 5 together form an azetidinyl, pyrrolidinyl or morpholino ring.
  • R 1 is selected from isopropyl, cyclopentyl, l,3-difluoroprop-2-yl, but-2-yl, 1- hydroxyprop-2-yl, 2-hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, tetrahydropyranyl, l-hydroxybut-2-yl and l-methoxyprop-2-yl;
  • HET-I is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
  • HET-2 is a substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
  • R 2 is -CONR 4 R 5 ;
  • R 4 and R 5 together form an azetidinyl or pyrrolidinyl ring.
  • R 1 is selected from isopropyl, l,3-difluoroprop-2-yl, l-hydroxyprop-2-yl and 1- methoxyprop-2-yl,
  • HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl and pyrazinyl, wherein R 1 is optionally substituted on carbon or nitrogen with a methyl or ethyl group;
  • HET-2 is selected from pyridyl, pyrazinyl, thiazolyl and thienyl, substituted by R 2 and optionally substituted by R 3 ;
  • R 3 is fluoro or chloro
  • R 2 is -CONR 4 R 5 ;
  • R 4 and R 5 together form an azetidinyl, pyrrolidinyl or morpholino ring.
  • R 1 is selected from isopropyl, l,3-difluoroprop-2-yl, but-2-yl, l-hydroxyprop-2-yl, 2- hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, l-hydroxybut-2-yl and 1- methoxyprop-2-yl;
  • HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl and pyrazinyl, wherein R 1 is optionally substituted with a methyl, isopropyl or ethyl group;
  • HET-2 is selected from pyridyl, pyrazinyl, thiazolyl and pyrimidinyl, substituted by R 2 and optionally substituted by R 3 ;
  • R 3 is fluoro or chloro;
  • R 2 is -CONR 4 R 5 ;
  • R 4 and R 5 together form an azetidinyl, pyrrolidinyl or morpholino ring.
  • R 1 is selected from isopropyl, cyclopentyl, l,3-difluoroprop-2-yl, but-2-yl, 1- hydroxyprop-2-yl, 2-hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, tetrahydropyranyl, l-hydroxybut-2-yl and l-methoxyprop-2-yl;
  • HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl, pyridyl, isoxazolyl and pyrazinyl, wherein R 1 is optionally substituted with a methyl, isopropyl or ethyl group and/or (on a carbon atom) by fluoro;
  • HET-2 is selected from pyridyl, pyrazinyl, pyridazinyl, thiazolyl and pyrimidinyl, substituted by R 2 and optionally substituted by R 3 ;
  • R 3 is fluoro or chloro
  • R 2 is -CONR 4 R 5 ;
  • R 4 and R 5 together form an azetidinyl or pyrrolidinyl ring.
  • R 1 is selected from isopropyl, cyclopentyl, l,3-difluoroprop-2-yl, but-2-yl, 1- hydroxyprop-2-yl, 2-hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, tetrahydropyranyl, l-hydroxybut-2-yl and l-methoxyprop-2-yl;
  • HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl, pyridyl, isoxazolyl and pyrazinyl, wherein R 1 is optionally substituted with a methyl, isopropyl or ethyl group and/or (on a carbon atom) by fluoro;
  • HET-2 is selected from pyridyl, pyrazinyl, pyridazinyl, thiazolyl and pyrimidinyl, substituted by R 2 and optionally substituted by R 3 ;
  • R 3 is methyl, fluoro or chloro;
  • R 2 is -CONR 4 R 5 ;
  • R 4 and R 5 together form an azetidinyl, 7-azabicyclo[2.2.1]hept-7-yl, morpholino, or pyrrolidinyl ring.
  • R 1 is selected from isopropyl, cyclopentyl, l,3-difluoroprop-2-yl, but-2-yl, 1- hydroxyprop-2-yl, 2-hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, tetrahydropyranyl, l-hydroxybut-2-yl and l-methoxyprop-2-yl;
  • HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl, pyridyl, isoxazolyl and pyrazinyl, wherein R 1 is optionally substituted with a methyl, isopropyl or ethyl group and/or (on a carbon atom) by fluoro;
  • HET-2 is selected from pyridyl, pyrazinyl, pyridazinyl, thiazolyl and pyrimidinyl, substituted by R 2 and optionally substituted by R 3 ;
  • R 3 is methyl, fluoro or chloro
  • R 2 is -CONR 4 R 5 ;
  • R 4 and R 5 together form an azetidinyl or pyrrolidinyl ring.
  • R 1 is selected from isopropyl, cyclopentyl, l,3-difluoroprop-2-yl, but-2-yl, 1- hydroxyprop-2-yl, 2-hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, tetrahydropyranyl, l-hydroxybut-2-yl and l-methoxyprop-2-yl;
  • HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl, pyridyl, isoxazolyl and pyrazinyl, wherein R 1 is optionally substituted on a nitrogen atom with methyl, difluoromethyl, isopropyl or ethyl and/or on a carbon atom by fluoro or methyl; provided that when HET-I is pyrazolyl, R 1 is selected from l-methoxyprop-2-yl, isopropyl, and tetrahydrofuryl;
  • HET-2 is selected from pyridyl, pyrazinyl, pyridazinyl, thiazolyl and pyrimidinyl, substituted by R 2 and optionally substituted by R 3 ;
  • R 3 is methyl, fluoro or chloro;
  • R 2 is -CONR 4 R 5 or -SO 2 NR 4 R 5 , particularly -CONR 4 R 5 ;
  • R 4 and R 5 together form an azetidinyl, 7-azabicyclo[2.2.1]hept-7-yl, morpholino, or pyrrolidinyl ring.
  • R 1 is selected from isopropyl, cyclopentyl, l,3-difluoroprop-2-yl, but-2-yl, 1- hydroxyprop-2-yl, 2-hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, tetrahydropyranyl, l-hydroxybut-2-yl and l-methoxyprop-2-yl;
  • HET-I is selected from pyrazinyl (optionally substituted with methyl), pyrazolyl (optionally substituted on carbon by methyl), methylthiadiazolyl (particularly 3-methyl- l,2,4-thiadiazol-5-yl), thiazolyl (optionally substituted with methyl), pyridyl (optionally substituted by fluoro) and isoxazolyl; provided that when HET-I is pyrazolyl, particularly
  • R 1 is selected from l-methoxyprop-2-yl, isopropyl, and tetrahydrofuryl;
  • HET-2 is selected from pyridyl, pyrazinyl, pyridazinyl, thiazolyl and pyrimidinyl, substituted by R 2 and optionally substituted by R 3 ;
  • R 3 is methyl, fluoro or chloro;
  • R 2 is -CONR 4 R 5 Or-SO 2 NR 4 R 5 , particularly -CONR 4 R 5 ;
  • R 4 and R 5 together form an azetidinyl, 7-azabicyclo[2.2.1]hept-7-yl, morpholino, or pyrrolidinyl ring.
  • R 1 is selected from isopropyl, cyclopentyl, l,3-difluoroprop-2-yl, but-2-yl, 1- hydroxyprop-2-yl, 2-hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, tetrahydropyranyl, l-hydroxybut-2-yl and l-methoxyprop-2-yl;
  • HET-I is selected from pyrazinyl (optionally substituted with methyl), pyrazolyl (optionally substituted on carbon by methyl), methylthiadiazolyl (particularly 3-methyl- l,2,4-thiadiazol-5-yl), thiazolyl (optionally substituted with methyl), pyridyl (optionally substituted by fluoro) and isoxazolyl; provided that when HET-I is pyrazolyl, particularly
  • R 1 is selected from l-methoxyprop-2-yl, isopropyl, and tetrahydrofuryl;
  • HET-2 is selected from pyridyl and pyrazinyl, substituted by R 2 and optionally substituted by R 3 ;
  • R 3 is methyl, fluoro or chloro
  • R 2 is -CONR 4 R 5 or -SO 2 NR 4 R 5 , particularly -CONR 4 R 5 ;
  • R 4 and R 5 together form an azetidinyl or pyrrolidinyl ring, particularly azetidinyl.
  • Aspect B is those of formulae (IB), (IC) and/or (ID).
  • Aspect C of the invention there is provided a compound of formula (IB), (IC) or (ID) as hereinbefore defined, or a salt thereof, wherein:
  • R 1 is selected from isopropyl, l-hydroxyprop-2-yl, tetrahydrofuryl and l-methoxyprop-2- yl; (particularly R 1 is selected from isopropyl, (2S)- l-hydroxyprop-2-yl, tetrahydrofuryl and (2S)- l-methoxyprop-2-yl); HET-I is methylpyrazinyl;
  • R 2 is -CONR 4 R 5 ;
  • R 1 is selected from isopropyl, l-hydroxyprop-2-yl, tetrahydrofuryl and l-methoxyprop-2- yl; (particularly R 1 is selected from isopropyl, (2S)- l-hydroxyprop-2-yl, tetrahydrofuryl and (2S)-l-methoxyprop-2-yl);
  • HET-I is methylpyrazinyl
  • R 2 is -CONR 4 R 5 ;
  • R 4 and R 5 together form an azetidinyl ring.
  • Aspect E of the invention there is provided a compound of formula (IB), (IC) or (ID) as hereinbefore defined, or a salt thereof, wherein:
  • R 1 is selected from isopropyl, tetrahydrofuryl and l-methoxyprop-2-yl (particularly (2S)-
  • HET-I is pyrazolyl
  • R 2 is -CONR 4 R 5 ; R 4 and R 5 together form an azetidinyl ring.
  • R 1 is selected from isopropyl, tetrahydrofuryl and l-methoxyprop-2-yl (particularly (2S)-
  • HET-I is pyrazolyl
  • R 2 is -CONR 4 R 5 ;
  • R 4 and R 5 together form an azetidinyl ring.
  • R 1 is selected from isopropyl, but-2-yl, l,3-difluoroprop-2-yl, l-hydroxyprop-2-yl and 1- methoxyprop-2-yl and tetrahydrofuryl;
  • HET-I is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
  • HET-2 is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
  • R 2 is -SO 2 NR 4 R 5 ;
  • R 4 and R 5 together form an azetidinyl, pyrrolidinyl or morpholino ring.
  • R 1 is selected from isopropyl, l,3-difluoroprop-2-yl, l-hydroxyprop-2-yl and 1- methoxyprop-2-yl, HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl and pyrazinyl, wherein R 1 is optionally substituted on carbon or nitrogen with a methyl or ethyl group;
  • HET-2 is selected from pyridyl, pyrazinyl, thiazolyl and thienyl, substituted by R 2 and optionally substituted by R 3 ;
  • R 3 is fluoro or chloro;
  • R 2 is -SO 2 NR 4 R 5 ;
  • R 4 and R 5 together form an azetidinyl, pyrrolidinyl or morpholino ring.
  • R 1 is selected from isopropyl, l,3-difluoroprop-2-yl, but-2-yl, l-hydroxyprop-2-yl, 2- hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, l-hydroxybut-2-yl and 1- methoxyprop-2-yl;
  • HET-I is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
  • HET-2 is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
  • R 2 Is -SO 2 NR 4 R 5 ;
  • R 4 and R 5 together form an azetidinyl, pyrrolidinyl or morpholino ring.
  • R 1 is selected from isopropyl, 1, 3 -difluoroprop-2-yl, but-2-yl, l-hydroxyprop-2-yl, 2- hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, l-hydroxybut-2-yl and 1- methoxyprop-2-yl;
  • HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl and pyrazinyl, wherein R 1 is optionally substituted with a methyl, isopropyl or ethyl group;
  • HET-2 is selected from pyridyl, pyrazinyl, thiazolyl and pyrimidinyl, substituted by R 2 and optionally substituted by R 3 ;
  • R 3 is fluoro or chloro
  • R 4 and R 5 together form an azetidinyl, pyrrolidinyl or morpholino ring.
  • R 1 is selected from isopropyl, l,3-difluoroprop-2-yl, but-2-yl, l-hydroxyprop-2-yl, 2- hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, l-hydroxybut-2-yl and 1- methoxyprop-2-yl;
  • HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl and pyrazinyl, wherein R 1 is optionally substituted with a methyl, isopropyl or ethyl group;
  • HET-2 is selected from pyridyl, pyrazinyl, thiazolyl and pyrimidinyl, substituted by R 2 and optionally substituted by R 3 ;
  • R 3 is fluoro or chloro
  • R 2 is -CONR 4 R 5 Or-SO 2 NR 4 R 5 ;
  • R 4 and R 5 together form an azetidinyl, pyrrolidinyl or morpholino ring.
  • R 1 is selected from l-fluoromethoxyprop-2-yl and 1, l-difluoromethoxyprop-2-yl;
  • HET-I is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined
  • HET-2 is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined
  • R 2 is -CONR 4 R 5 ;
  • R 4 and R 5 together form an azetidinyl, pyrrolidinyl or morpholino ring.
  • R 1 is selected from l-fluoromethoxyprop-2-yl and 1, l-difluoromethoxyprop-2-yl;
  • HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl and pyrazinyl, wherein R 1 is optionally substituted on carbon or nitrogen with a methyl or ethyl group;
  • HET-2 is selected from pyridyl, pyrazinyl, thiazolyl and thienyl, substituted by R 2 and optionally substituted by R 3 ;
  • R 3 is fluoro or chloro
  • R 2 is -CONR 4 R 5 ; R 4 and R 5 together form an azetidinyl, pyrrolidinyl or morpholino ring.
  • R 1 is selected from l-fiuoromethoxy ⁇ rop-2-yl and l,l-difluoromethoxyprop-2-yl;
  • HET-I is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
  • HET-2 is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
  • R 2 is -SO 2 NR 4 R 5 ; R 4 and R 5 together form an azetidinyl, pyrrolidinyl or morpholino ring.
  • R 1 is selected from l-fluoromethoxyprop-2-yl and l,l-difluoromethoxyprop-2-yl;
  • HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl and pyrazinyl, wherein R 1 is optionally substituted on carbon or nitrogen with a methyl or ethyl group;
  • HET-2 is selected from pyridyl, pyrazinyl, thiazolyl and thienyl, substituted by R 2 and optionally substituted by R 3 ;
  • R 3 is fluoro or chloro
  • R 2 is -SO 2 NR 4 R 5 ; R 4 and R 5 together form an azetidinyl, pyrrolidinyl or morpholino ring.
  • Further preferred compounds of the invention are each of the Examples (and salts thereof), each of which provides a further independent aspect of the invention.
  • the present invention also comprises any two or more compounds of the Examples (and salts thereof).
  • Particular compounds of the invention include any one or more of:
  • N-l,3-thiazol-2-ylbenzamide 3- ⁇ [5-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy ⁇ -5- ⁇ [(lS)-2-hydroxy-l-methylethyl]oxy ⁇ -
  • N-(5-methylpyrazin-2-yl)benzamide 3- ⁇ [5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy ⁇ -5-( ⁇ (lS)-2-[(difluoromethyl)oxy]-l- methylethyl ⁇ oxy)-N- 1 H-pyrazol-3 -ylbenzamide;
  • a further feature of the invention is a pharmaceutical composition
  • a pharmaceutical composition comprising a compound of Formula (I), (IA), (IB), (IC) or (ID) as defined above, or a pharmaceutically- acceptable salt thereof, together with a pharmaceutically-acceptable diluent or carrier.
  • the a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically-acceptable salt thereof as defined above for use as a medicament for treatment of a disease mediated through GLK, in particular type 2 diabetes.
  • a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically-acceptable salt thereof in the preparation of a medicament for treatment of a disease mediated through GLK, in particular type 2 diabetes.
  • the compound is suitably formulated as a pharmaceutical composition for use in this way.
  • a method of treating GLK mediated diseases, especially diabetes by administering an effective amount of a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically-acceptable salt thereof, to a mammal in need of such treatment.
  • Specific diseases which may be treated by a compound or composition of the invention include: blood glucose lowering in Type 2 Diabetes Mellitus without a serious risk of hypoglycaemia (and potential to treat type 1), dyslipidemia, obesity, insulin resistance, metabolic syndrome X, impaired glucose tolerance.
  • the GLK/GLKRP system can be described as a potential "Diabesity" target (of benefit in both Diabetes and Obesity).
  • a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically-acceptable salt thereof in the preparation of a medicament for use in the combined treatment or prevention, particularly treatment of diabetes and obesity.
  • a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically-acceptable salt thereof in the preparation of a medicament for use in the treatment or prevention, particularly treatment of obesity.
  • the a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically-acceptable salt thereof as defined above for use as a medicament for treatment or prevention, particularly treatment of obesity.
  • a method for the combined treatment of obesity and diabetes by administering an effective amount of a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically-acceptable salt thereof, to a mammal in need of such treatment.
  • a method for the treatment of obesity by administering an effective amount of a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically-acceptable salt thereof, to a mammal in need of such treatment.
  • Compounds of the invention may be particularly suitable for use as pharmaceuticals because of advantageous physical and/or pharmacokinetic properties, and/or favourable toxicity profile and/or favourable metabolic profile.
  • Favourable toxicity profile may be demonstrated, for example, by use of an Ames test assay, and/or by testing against the hERG ion channel.
  • a favourable metabolic profile may mean, for example, reduced rate of metabolism, leading to reduction in clearance of the compound from the body and hence increased exposure to the compound, or a favourable metabolic profile may mean, for example, not forming active metabolites (which might be considered undesirable in some circumstances).
  • compounds of Aspects A to F may have favourable toxicological profiles.
  • compositions of the invention may be in a form suitable for oral use (for example as tablets, lozenges, hard or soft capsules, aqueous or oily suspensions, emulsions, dispersible powders or granules, syrups or elixirs), for topical use (for example as creams, ointments, gels, or aqueous or oily solutions or suspensions), for administration by inhalation (for example as a finely divided powder or a liquid aerosol), for administration by insufflation (for example as a finely divided powder) or for parenteral administration (for example as a sterile aqueous or oily solution for intravenous, subcutaneous, intramuscular or intramuscular dosing or as a suppository for rectal dosing). Dosage forms suitable for oral use are preferred.
  • compositions of the invention may be obtained by conventional procedures using conventional pharmaceutical excipients, well known in the art.
  • compositions intended for oral use may contain, for example, one or more colouring, sweetening, flavouring and/or preservative agents.
  • Suitable pharmaceutically acceptable excipients for a tablet formulation include, for example, inert diluents such as lactose, sodium carbonate, calcium phosphate or calcium carbonate, granulating and disintegrating agents such as corn starch or algenic acid; binding agents such as starch; lubricating agents such as magnesium stearate, stearic acid or talc; preservative agents such as ethyl or propyl p .
  • Tablet formulations may be uncoated or coated either to modify their disintegration and the subsequent absorption of the active ingredient within the gastrointestinal tract, or to improve their stability and/or appearance, in either case, using conventional coating agents and procedures well known in the art.
  • Compositions for oral use may be in the form of hard gelatin capsules in which the active ingredient is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin, or as soft gelatin capsules in which the active ingredient is mixed with water or an oil such as peanut oil, liquid paraffin, or olive oil.
  • an inert solid diluent for example, calcium carbonate, calcium phosphate or kaolin
  • water or an oil such as peanut oil, liquid paraffin, or olive oil.
  • Aqueous suspensions generally contain the active ingredient in finely powdered form together with one or more suspending agents, such as sodium carboxymethylcellulose, methylcellulose, hydroxypropylmethylcellulose, sodium alginate, polyvinyl-pyrrolidone, gum tragacanth and gum acacia; dispersing or wetting agents such as lecithin or condensation products of an alkylene oxide with fatty acids (for example polyoxethylene stearate), or condensation products of ethylene oxide with long chain aliphatic alcohols, for example heptadecaethyleneoxycetanol, or condensation products of ethylene oxide with partial esters derived from fatty acids and a hexitol such as polyoxyethylene sorbitol monooleate, or condensation products of ethylene oxide with long chain aliphatic alcohols, for example heptadecaethyleneoxycetanol, or condensation products of ethylene oxide with partial esters derived from fatty acids and a hexitol such as polyoxyethylene sorbitol
  • the aqueous suspensions may also contain one or more preservatives (such as ethyl or propyl r)-hydroxybenzoate, anti-oxidants (such as ascorbic acid), colouring agents, flavouring agents, and/or sweetening agents (such as sucrose, saccharine or aspartame).
  • preservatives such as ethyl or propyl r
  • anti-oxidants such as ascorbic acid
  • colouring agents such as ascorbic acid
  • flavouring agents such as as ascorbic acid
  • sweetening agents such as sucrose, saccharine or aspartame
  • Oily suspensions may be formulated by suspending the active ingredient in a vegetable oil (such as arachis oil, olive oil, sesame oil or coconut oil) or in a mineral oil (such as liquid paraffin).
  • the oily suspensions may also contain a thickening agent such as beeswax, hard paraffin or cetyl alcohol.
  • Sweetening agents such as those set out above, and flavouring agents may be added to provide a palatable oral preparation.
  • These compositions may be preserved by the addition of an anti-oxidant such as ascorbic acid.
  • Dispersible powders and granules suitable for preparation of an aqueous suspension by the addition of water generally contain the active ingredient together with a dispersing or wetting agent, suspending agent and one or more preservatives. Suitable dispersing or wetting agents and suspending agents are exemplified by those already mentioned above. Additional excipients such as sweetening, flavouring and colouring agents, may also be present.
  • the pharmaceutical compositions of the invention may also be in the form of oil-in- water emulsions.
  • the oily phase may be a vegetable oil, such as olive oil or arachis oil, or a mineral oil, such as for example liquid paraffin or a mixture of any of these.
  • Suitable emulsifying agents may be, for example, naturally-occurring gums such as gum acacia or gum tragacanth, naturally-occurring phosphatides such as soya bean, lecithin, an esters or partial esters derived from fatty acids and hexitol anhydrides (for example sorbitan monooleate) and condensation products of the said partial esters with ethylene oxide such as polyoxyethylene sorbitan monooleate.
  • the emulsions may also contain sweetening, flavouring and preservative agents.
  • Syrups and elixirs may be formulated with sweetening agents such as glycerol, propylene glycol, sorbitol, aspartame or sucrose, and may also contain a demulcent, preservative, flavouring and/or colouring agent.
  • the pharmaceutical compositions may also be in the form of a sterile injectable aqueous or oily suspension, which may be formulated according to known procedures using one or more of the appropriate dispersing or wetting agents and suspending agents, which have been mentioned above.
  • a sterile injectable preparation may also be a sterile injectable solution or suspension in a non-toxic parenterally-acceptable diluent or solvent, for example a solution in 1,3-butanediol.
  • Compositions for administration by inhalation may be in the form of a conventional pressurised aerosol arranged to dispense the active ingredient either as an aerosol containing finely divided solid or liquid droplets.
  • Conventional aerosol propellants such as volatile fluorinated hydrocarbons or hydrocarbons may be used and the aerosol device is conveniently arranged to dispense a metered quantity of active ingredient.
  • the amount of active ingredient that is combined with one or more excipients to produce a single dosage form will necessarily vary depending upon the host treated and the particular route of administration.
  • a formulation intended for oral administration to humans will generally contain, for example, from 0.5 mg to 2 g of active agent compounded with an appropriate and convenient amount of excipients which may vary from about 5 to about 98 percent by weight of the total composition.
  • Dosage unit forms will generally contain about 1 mg to about 500 mg of an active ingredient.
  • the size of the dose for therapeutic or prophylactic purposes of a compound of the Formula (I), (IA), (IB), (IC) or (ID) will naturally vary according to the nature and severity of the conditions, the age and sex of the animal or patient and the route of administration, according to well known principles of medicine.
  • a daily dose in the range for example, 0.5 mg to 75 mg per kg body weight is received, given if required in divided doses.
  • a parenteral route is employed.
  • a dose in the range for example, 0.5 mg to 30 mg per kg body weight will generally be used.
  • a dose in the range for example, 0.5 mg to 25 mg per kg body weight will be used.
  • Oral administration is however preferred.
  • the elevation of GLK activity described herein may be applied as a sole therapy or in combination with one or more other substances and/or treatments for the indication being treated. Such conjoint treatment may be achieved by way of the simultaneous, sequential or separate administration of the individual components of the treatment. Simultaneous treatment may be in a single tablet or in separate tablets.
  • chemotherapy may include the following main categories of treatment:
  • Insulin secretagogues including sulphonylureas (for example glibenclamide, glipizide), prandial glucose regulators (for example repaglinide, nateglinide);
  • Agents that improve incretin action for example dipeptidyl peptidase IV inhibitors, and GLP-I agonists);
  • Insulin sensitising agents including PPARgamma agonists (for example pioglitazone and rosiglitazone), and agents with combined PPARalpha and gamma activity;
  • Agents that modulate hepatic glucose balance for example metformin, fructose 1, 6 bisphosphatase inhibitors, glycogen phopsphorylase inhibitors, glycogen synthase kinase inhibitors);
  • SGLT inhibitors Agents that prevent the reabsorption of glucose by the kidney (SGLT inhibitors); 8) Agents designed to treat the complications of prolonged hyperglycaemia (for example aldose reductase inhibitors);
  • Anti-obesity agents for example sibutramine and orlistat
  • Anti- dyslipidaemia agents such as, HMG-CoA reductase inhibitors (eg statins); PP ARa agonists (fibrates, eg gemfibrozil); bile acid sequestrants (cholestyramine); cholesterol absorption inhibitors (plant stanols, synthetic inhibitors); bile acid absorption inhibitors (IBATi) and nicotinic acid and analogues (niacin and slow release formulations);
  • Antihypertensive agents such as, ⁇ blockers (eg atenolol, inderal); ACE inhibitors (eg lisinopril); Calcium antagonists (eg. nifedipine); Angiotensin receptor antagonists (eg candesartan), ⁇ antagonists and diuretic agents (eg. furosemide, benzthiazide); 12) Haemostasis modulators such as, antithrombotics, activators of fibrinolysis and antiplatelet agents; thrombin antagonists; factor Xa inhibitors; factor Vila inhibitors); antiplatelet agents (eg. aspirin, clopidogrel); anticoagulants (heparin and Low molecular weight analogues, hirudin) and warfarin;
  • ⁇ blockers eg atenolol, inderal
  • ACE inhibitors eg lisinopril
  • Calcium antagonists eg. nifedipine
  • Anti-inflammatory agents such as non-steroidal anti-inflammatory drugs (eg. aspirin) and steroidal anti-inflammatory agents (eg. cortisone).
  • a compound of the invention, or a salt thereof may be prepared by any process known to be applicable to the preparation of such compounds or structurally related compounds.
  • Functional groups may be protected and deprotected using conventional methods.
  • protecting groups such as amino and carboxylic acid protecting groups (as well as means of formation and eventual deprotection), see T. W. Greene and P.G.M. Wuts, "Protective Groups in Organic Synthesis", Second Edition, John Wiley & Sons, New York, 1991.
  • Processes for the synthesis of compounds of Formula (I), (IA), (IB), (IC) or (ID) are provided as a further feature of the invention.
  • a process for the preparation of a compound of Formula (I), (IA), (IB), (IC) or (ID) which comprises a process a) to e) (wherein the variables are as defined hereinbefore for compounds of Formula (I), (IA), (IB), (IC) or (ID) unless otherwise defined):
  • Suitable leaving groups X 1 to X 5 for processes b) to d) are any leaving group known in the art for these types of reactions, for example halo, alkoxy, trifluoromethanesulfonyloxy, methanesulfonyloxy, or p-toluenesulfonyloxy; or a group (such as a hydroxy group) that may be converted into a leaving group (such as an oxytriphenylphosphonium group) in situ.
  • Suitable values for R 1 containing a protected hydroxy group are any suitable protected hydroxy group known in the art, for example simple ethers such as a methyl ether, tert-butyl ether or silylethers such as -OSi[(l-4C)alkyl] 3 (wherein each (l-4C)alkyl group is independently selected from methyl, ethyl, propyl, isopropyl, and tertbutyl).
  • trialkylsilyl groups are trimethylsilyl, triethylsilyl, triisopropylsilyl and tert-butyldimethylsilyl.
  • Compounds of Formula (XIII) may be made by processes such as those shown in processes a) to d) and/or by those processes mentioned above for compounds of formulae (III) to (XII).
  • the group R 1 in the compounds of formulae (III), (IX), (X), (XI) and (XIII) may be made by reaction of suitable precursors with compounds of formula (V) or derivatives thereof, depending on the nature of the R 1 group, for example, by nucleophilic displacement of a leaving group X 1 in a compound of formula (V).
  • Compounds of formula (V) are generally commercially available or maybe made by simple functional group interconversions from commercially available compounds, or by literature methods. Further information is available in WO2004/076420, WO2005/054200, WO2005/054233, WO 2005/044801 and WO 2005/056530.
  • Further examples of a conversion of a compound of Formula (I) into another compound of Formula (I) is methylation of a hydroxy group in R 1 to give a methoxy group, and conversion of, for example, a hydroxymethyl group in R 1 (such as when R 1 is hydroxyprop-2-yl) into a difluoromethoxy group, using reactions such as those as illustrated in Scheme 4.
  • substituents R 3 , R 6 and/or R 7 may be introduced into the molecule at any convenient point in the synthetic sequence or may be present in the starting materials.
  • a precursor to one of these substituents may be present in the molecule during the process steps a) to e) above, and then be transformed into the desired substituent as a final step to form the compound of formula (I); followed where necessary by i) converting a compound of Formula (I) into another compound of Formula (I); ii) removing any protecting groups; and/or iii) forming a salt thereof.
  • an appropriate coupling reaction such as a carbodiimide coupling reaction performed with EDAC (l-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) in the presence of dimethylaminopyridine (DMAP) in a suitable solvent such as dichloromethane (DCM), chloroform or dimethylformamide (DMF) at room temperature; or
  • EDAC l-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride
  • DMAP dimethylaminopyridine
  • DCM dichloromethane
  • DMF dimethylformamide
  • Process b) - compounds of Formula (V) and (VI) can be reacted together in a suitable solvent, such as DMF or tetrahydrofuran (THF), with a base such as sodium hydride or potassium tert-butoxide, at a temperature in the range 0 to 200 0 C, optionally using microwave heating or metal catalysis such as palladium(II)acetate, palladium on carbon, copper(II)acetate or copper(I)iodide; alternatively, compounds of Formula (V) and (VI) can be reacted together in a suitable solvent, such as THF or DCM, with a suitable phosphine such as triphenylphosphine, and azodicarboxylate such as diethylazodicarboxylate; process b) could also be carried out using a precursor to the ester of formula (VII) such as an aryl-nitrile or trifluoromethyl derivative, followed by conversion to a carboxylic acid and amide formation
  • protecting groups may be removed by any convenient method as described in the literature or known to the skilled chemist as appropriate for the removal of the protecting group in question, such methods being chosen so as to effect removal of the protecting group with minimum disturbance of groups elsewhere in the molecule.
  • Specific examples of protecting groups are given below for the sake of convenience, in which "lower” signifies that the group to which it is applied preferably has 1-4 carbon atoms. It will be understood that these examples are not exhaustive. Where specific examples of methods for the removal of protecting groups are given below these are similarly not exhaustive. The use of protecting groups and methods of deprotection not specifically mentioned is of course within the scope of the invention.
  • a carboxy protecting group may be the residue of an ester-forming aliphatic or araliphatic alcohol or of an ester-forming silanol (the said alcohol or silanol preferably containing 1-20 carbon atoms).
  • carboxy protecting groups include straight or branched chain (l-12C)alkyl groups (e.g. isopropyl, t-butyl); lower alkoxy lower alkyl groups (e.g. methoxymethyl, ethoxymethyl, isobutoxymethyl); lower aliphatic acyloxy lower alkyl groups, (e.g. acetoxymethyl, propionyloxymethyl, butyryloxymethyl, pivaloyloxymethyl); lower alkoxycarbonyloxy lower alkyl groups (e.g.
  • aryl lower alkyl groups e.g. p_-methoxybenzyl, o-nitrobenzyl, ⁇ -nitrobenzyl, benzhydryl and phthalidyl
  • tri(lower alkyl)silyl groups e.g. trimethylsilyl and t-butyldimethylsilyl
  • tri(lower alkyl)silyl lower alkyl groups e.g. trimethylsilylethyl
  • (2-6C)alkenyl groups e.g. allyl and vinylethyl.
  • hydroxy protecting groups include methyl, t-butyl, lower alkenyl groups (e.g. allyl); lower alkanoyl groups (e.g. acetyl); lower alkoxycarbonyl groups (e.g. t-butoxycarbonyl); lower alkenyloxycarbonyl groups (e.g. allyloxycarbonyl); aryl lower alkoxycarbonyl groups (e.g. benzoyloxycarbonyl, rj-methoxybenzyloxycarbonyl, o-nitrobenzyloxycarbonyl, p_-nitrobenzyloxycarbonyl); tri lower alkyl/arylsilyl groups (e.g.
  • amino protecting groups include formyl, aralkyl groups (e.g. benzyl and substituted benzyl, e.g. ⁇ -methoxybenzyl, nitrobenzyl and 2,4-dimethoxybenzyl, and triphenylmethyl); di-p_-anisylmethyl and furylmethyl groups; lower alkoxycarbonyl (e.g.
  • t-butoxycarbonyl lower alkenyloxycarbonyl (e.g. allyloxycarbonyl); aryl lower alkoxycarbonyl groups (e.g. benzyloxycarbonyl, ⁇ _-methoxybenzyloxycarbonyl, o-nitrobenzyloxycarbonyl, p_-nitrobenzyloxycarbonyl; trialkylsilyl (e.g. trimethylsilyl and t-butyldimethylsilyl); alkylidene (e.g. methylidene); benzylidene and substituted benzylidene groups.
  • aryl lower alkoxycarbonyl groups e.g. benzyloxycarbonyl, ⁇ _-methoxybenzyloxycarbonyl, o-nitrobenzyloxycarbonyl, p_-nitrobenzyloxycarbonyl
  • trialkylsilyl e.g. trimethylsilyl and t-butyldimethylsily
  • Methods appropriate for removal of hydroxy and amino protecting groups include, for example, hydrogenation, nucleophilic displacement, acid-, base, metal- or enzymically-catalysed hydrolysis, catalytic hydrogenolysis or photolytically for groups such as o-nitrobenzyloxycarbonyl, or with fluoride ions for silyl groups.
  • methylether protecting groups for hydroxy groups may be removed by trimethylsilyliodide.
  • a tert-butyl ether protecting group for a hydroxy group may be removed by hydrolysis, for example by use of hydrochloric acid in methanol.
  • protecting groups for amide groups include aralkoxymethyl (e.g. ben ⁇ yloxymethyl and substituted ben2yloxymethyl); alkoxymethyl (e.g. methoxymethyl and trimethylsilylethoxymethyl); tri alkyl/arylsilyl (e.g. trimethylsilyl, t-butyldimethylsily, t-butyldiphenylsilyl); tri alkyl/arylsilyloxymethyl (e.g. t-butyldimethylsilyloxymethyl, t-butyldiphenylsilyloxymethyl); 4-alkoxyphenyl (e.g.
  • Aralkoxymethyl, groups may be introduced onto the amide group by reacting the latter group with the appropriate aralkoxymethyl chloride, and removed by catalytic hydrogenation.
  • Alkoxymethyl, tri alkyl/arylsilyl and tri alkyl/silyloxymethyl groups may be introduced by reacting the amide with the appropriate chloride and removing with acid; or in the case of the silyl containing groups, fluoride ions.
  • the alkoxyphenyl and alkoxybenzyl groups are conveniently introduced by arylation or alkylation with an appropriate halide and removed by oxidation with eerie ammonium nitrate.
  • alk-1- enyl groups may be introduced by reacting the amide with the appropriate aldehyde and removed with acid.
  • Purification by chromatography generally refers to flash column chromatography, on silica unless otherwise stated. Column chromatography was generally carried out using prepacked silica cartridges (from 4g up to 40Og) such as RedisepTM
  • SPE Solid Phase Extraction
  • MS Mass spectra
  • HPLC component comprised generally either a Agilent 1100 or Waters Alliance HT (2790 & 2795) equipment and was run on a Phemonenex Gemini Cl 8 5 ⁇ m, 50 x 2 mm column (or similar) eluting with either acidic eluent (for example, using a gradient between 0 - 95% water / acetonitrile with 5% of a 1% formic acid in 50:50 water: acetonitrile (v/v) mixture; or using an equivalent solvent system with methanol instead of acetonitrile), or basic eluent (for example, using a gradient between 0 - 95% water / acetonitrile with 5% of a 0.1% 880 Ammonia in acetonitrile mixture); and the MS component comprised generally a Waters ZQ spectrometer.
  • Suitable microwave reactors include "Smith Creator”, “CEM Explorer”, “Biotage Initiator sixty” and “Biotage Initiator eight”.
  • Potassium carbonate (0.14 g, 0.98 mmol) was added to a mixture of 3-[((15)-2- ⁇ [(l,l- dimethylethyl)(dimethyl)silyl] oxy ⁇ - 1 -methylethyl)oxy] -5-hydroxy-N- 1 ,3 -thiazol-2- ylbenzamide (200 mg, 0.49 mmol) and 5-(azetidin-l-ylcarbonyl)-2-chloropyridine (96 mg, 0.49 mmol) in acetonitrile (5.0 mL) and the stirred mixture heated at 16O 0 C in a 'Biotage initiator Microwave' for 4 hours.
  • Example Ia The precursor for Example Ia was prepared in a similar fashion from 3-[((liS)-2- ⁇ [(l,l- dimethylethyl)(dimethyl)silyl]oxy ⁇ - 1 -methylethyl)oxy]-5-hydroxy-N- 1 ,3-thiazol-2- ylbenzamide using 5-(azetidin-l-ylcarbonyl)-2,3-dichloropyridine:
  • Example Ib The precursor for Example Ib was prepared as described below: 3- ⁇ [6-(Azetidin-l-ylcarbonvnpyridin-3-yl1oxyl-5-rr(l ⁇ -2-irn.l- dimethylethviydimethv ⁇ silylloxy)-l-methylethyl)oxy1-A/-l,3-thiazol-2-ylbenzamide
  • Bromotris(triphenylphosphine)copper may be prepared according to Synthetic Communications, 31(18), 2865-2879 (2001). The preparation of bromotris(triphenylphosphine)copper is described below.
  • the residual acid chloride was dissolved in DCM (10 mL) and added to a mixture of azetidine hydrochloride (0.66 g, 6.99 mmol) and triethylamine (2.14 mL, 13.97 mmol) in DCM (10 mL) then stirred at RT for 24 hours.
  • the DCM was evaporated in vacuo, and the residue partitioned between ethyl acetate (100 mL) and IM citric acid (50 mL).
  • halopyridines used in the preparation of precursors for Examples la-Id were prepared in a similar fashion from the appropriate nicotinic acid:
  • the nicotinic acids were either commercially available compounds or compounds known in the literature. Reference for 2-(azetidin-l-ylcarbonyl)-5-bromopyridine (Heterocycles, 15(1) 1981, 213-23). The preparation of 3-[((15)-2- ⁇ [(I , l-dimethylethyl)(dimethyl)silyl]oxy ⁇ - 1 - methylethyl)oxy]-5-hydroxy-N-l,3-thiazol-2-ylbenzamide used in the synthesis of Example 1, Ia and Ib is described below:
  • tert-Butyldimethylsilyl chloride (1.66 g, 11.0 mmol) and imidazole (1.78 g, 26.0 mmol) were a to a solution of 3-hydroxy-5- ⁇ [(liS)-2-hydroxy-l-methylethyl]oxy ⁇ -N-l,3-thiazol-2- ylbenzamide (1.541 g, 5.24 mtnol)in DMF (10 mL) and the reaction stirred at RT under a silica drying tube overnight. Water (30 mL) was added and the solution partitioned with ethyl acetate (100 mL).
  • the DCM and excess oxalyl chloride were removed in vacuo, the residual oil dissolved in DCM (25 mL) and added to a solution of 2-aminothiazole (2.84g) and triethylamine (7.88 mL) in DCM (75 mL) at 0-5 0 C, and the mixture stirred at ambient temperature for 4 hours.
  • the DCM and excess triethylamine were removed in vacuo, the residual oil partitioned between ethyl acetate (100 mL) and IM hydrochloric acid (100 mL).
  • reaction mixture was stirred at ambient temperature for 16 hours, filtered through diatomaceous earth and the DCM evaporated to a residue which was chromatographed on silica with 10% ethyl acetate in isohexane as eluant to give the desired compound (10.7 g).
  • Lithium hydroxide monohydrate (12.14 g, 0.289 mol) in water (100 mL) was added to a solution of methyl 3-(benzyloxy)-5- ⁇ (l>S)-l-methyl-2- [(triisopropylsilyl)oxy]ethoxy ⁇ benzoate (62 g, 0.131 mol) in THF (300 mL) and warmed to
  • Triisopropylsilyl chloride (83.8 mL, 390 mmol) was added slowly over 15 minutes to a solution of (2i?)-pro ⁇ ane-l,2-diol (29.7 g, 390 mmol) in DMF at 0 0 C (100 mL) keeping the internal temperature below 15 0 C. This was followed by addition of imidazole (66.4 g, 975 mmol) and the reaction mixture was allowed to warm to RT and stirred under argon for 20 hours. The reaction was quenched with IM hydrochloric acid/diethyl ether (300 mL/800 mL). The organic layer was separated and washed with IM hydrochloric acid followed by saturated brine solution.
  • Potassium carbonate (0.181 g, 1.31 mmol) was added to a mixture of 3-hydroxy-5-[(15)-2- methoxy-(l-methylethyl)oxy]-N-(l-methyl-lH-pyrazol-3-yl)benzamide (0.2 g, 0.66 mmol) and 5-(azetidin-l-ylcarbonyl)-2-chloropyridine (129 mg, 0.66 mmol) in acetonitrile (5.0 mL) and the stirred mixture heated at 16O 0 C in a 'Biotage initiator Microwave' for 6 hours.
  • Examples 2a-2g were prepared using the appropriate phenol and halopyridine: 4.55
  • the resulting aqueous solution was acidified to pH4 with 2M hydrochloric acid solution and extracted with ethyl acetate (2 x 200 mL). The extracts were combined, washed with brine, dried (MgSO 4 ), and evaporated to give the desired compound (99% yield).
  • the solid was dissolved in ethyl acetate (200 mL) and washed with aqueous saturated sodium hydrogen carbonate solution (100 mL). The organics were washed with water (100 mL) and brine (100 mL), and dried (MgSO 4 ) before evaporation in vacuo to afford a solid which was washed with isohexane (200 mL) and dried in vacuo to give the desired compound (7.18 g).
  • the aqueous layer was extracted with further ethyl acetate (300 mL), and the combined organics washed with brine (75 mL), and dried (MgSO 4 ), before evaporation in vacuo to give the desired compound (28 g) which was used without further purification.
  • the 1 H NMR spectrum also contained signals consistent with a small amount of ethyl acetate.
  • the DCM and excess oxalyl chloride were removed in vacuo, the residual oil dissolved in DCM (50 mL) and added to a solution of 5-amino-3-methyl-l,2,4 thiadiazole (6.05 g) and triethylamine (14.6 mL) in DCM (150 mL) at 0-5 0 C, and the mixture stirred at ambient temperature for 16 hours.
  • the DCM and excess triethylamine were removed in vacuo, and the residual oil partitioned between ethyl acetate (250 mL) and IM hydrochloric acid (150 mL).
  • the ethyl acetate layer was separated, washed sequentially with IM hydrochloric acid, aqueous sodium hydrogen carbonate solution, and brine, dried (MgSO 4 ), and evaporated to a residue which was chromatographed on alumina with ethyl acetate as eluant, then on silica with 30% ethyl acetate in isohexane as eluant to give the desired compound (9.6 g).
  • the mixture was allowed to reach RT and pressure and was partitioned between ethyl acetate (50 mL) and water (50 mL).
  • the ethyl acetate layer was separated, washed with water (5 x 50 mL), brine (50 mL), dried (MgSO 4 ) and evaporated to a residue which was chromatographed on silica, eluting with a gradient of 0-5% methanol in ethyl acetate, to give the desired compound (113 mg).
  • the compound was partially crystallised from methanol.
  • the material was also partially crystallised from isopropyl alcohol / isohexane or ethyl acetate / isohexane.
  • the DCM was removed in vacuo, and the residue partitioned between ethyl acetate (400 mL) and IN hydrochloric acid (200 mL). The ethyl acetate layer was washed sequentially with saturated aqueous sodium hydrogen carbonate (200 mL) and brine (100 mL), dried
  • Triethylamine (0.025 mL, 0.18 mmol) then triethylsilane (0.96 mL, 6.03 mmol) were added to palladium (II) acetate (12 mg) in DCM (2 mL) and stirred under argon for 15 mins.
  • Azetidine hydrochloride (0.32 g, 3.44 mmol) was added to a solution of 3-bromo-2- chloropyridine-5-sulfonyl chloride (1 g, 3.44 mmol) in DCM (4 mL) and pyridine (10 mL) and stirred at RT for 22 hours. The solvent was removed in vacuo and ethyl acetate (30 mL) added. The organic phase was washed with IM hydrochloric acid (20 mL), water (20 mL), brine (20 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give the desired compound (0.4 g).
  • DIPEA (0.28 mL, 1.59 mmol) was added to a suspension of 5-[(3- ⁇ [(lS)-l-methyl-2- (methyloxy)ethyl] oxy ⁇ -5- ⁇ [( 1 -methyl- lH-pyrazol-3 - yl)amino]carbonyl ⁇ phenyl)oxy]pyrazine-2-carboxylic acid (170 mg, 0.4 mmol), ⁇ ATU (318 mg, 0.84 mmol) and azetidine hydrochloride (75 mg, 0.8 mmol) in DMF (5 mL) and the mixture stirred at RT for 72 hours.
  • the T ⁇ F was removed in vaccuo and the resulting solution was partitioned between water (50 mL) and ethyl acetate (75 mL), then the ethyl acetate layer separated, washed with brine and dried (MgSO 4 ). The aqueous layer was then taken to p ⁇ 7 by addition of IN hydrochloric acid (5.2 mL) and extracted with ethyl acetate (75 mL). The ethyl acetate layer was separated, washed with brine and dried (MgSO 4 ). The ethyl acetate layers were combined and evaporated to give the required product (0.84 g).
  • DIPEA (4.06 g 5 23.4 mmol) was added to a suspension of 3-[((lS)-2- ⁇ [(l,l- dimethylethyl)(dimethyl)silyl]oxy ⁇ -l-methylethyl)oxy]-5-[(phenylmethyl)oxy]benzoic acid (2.43 g, 5.84 mmol), 3-amino-l-methylpyrazole (0.85 g, 8.76 mmol) and ⁇ ATU (4.66 g, 12.3 mmol) in DMF (50 mL) and stirred at ambient temperature for 16 hours. The resultant mixture was partially reduced in vacuo, poured onto water (100 mL) and extracted with diethyl ether (2 x 50 mL).
  • fert-Butyl(dimethyl)silyl chloride (5.90 g, 39.5 mmol) was added to a solution of (2R)- pro ⁇ ane-l,2-diol (3.00 g, 39.5 mmol) in DCM (100 mL) followed by diisopropylethylamine (7.10 g, 55.3 mmol) and the reaction was stirred under argon for 72 h. The reaction was diluted with diethyl ether (500 mL) and water (140 mL) and the organic layer was separated then dried (MgSO 4 ), filtered and evaporated.
  • Example 8 S-ffS-Chloro-S-dnorpholin ⁇ -ylcarbonvDpyridin-l-vnoxyl-S-irdS) ⁇ - hvdroxy-l-methvIethylloxy>-iV-(l-methvI-lH/-pyrazoI-3-yl)benzamide
  • the T ⁇ F was removed in vaccuo and the resulting solution was partitioned between water (50 mL) and ethyl acetate (75 mL), and the ethyl acetate layer separated, washed with brine and dried (MgSO 4 ).
  • the aqueous layer was then adjusted to p ⁇ 7 by addition of IN hydrochloric acid (5.2 mL) and partitioned between ethyl acetate (75 mL).
  • the ethyl acetate layer was separated, washed with brine and dried (MgSO 4 ).
  • the ethyl acetate layers were combined and evaporated to give the required product (1.1 g).
  • HATU (1.19 g, 3.13 mmol) was added to 3- ⁇ [(lS)-l-methyl ⁇ ropyl]oxy ⁇ -5- [(phenylmethyl)oxy]benzoic acid (750 mg, 2.5 mmol) and 3-amino-l methyl pyrazole (291 mg, 3 mmol) in DMF (5 mL) followed by addition of DIPEA (0.807 mL, 6.25 mmol) and the reaction was stirred for 16 hours.
  • the reaction mixture was added to ethyl acetate (30 mL), washed with water (10 mL), 2N citric acid (10 mL), saturated aqueous sodium hydrogencarbonate (10 mL) and brine (10 mL), then dried (MgSO 4 ), filtered and evaporated to a gummy residue.
  • the residue was taken up in 50% ether in ethylacetate (50 mL), washed with 2N hydrochloric acid (10 mL), water (10 mL), saturated aqueous sodium hydrogencarbonate (10 mL) and brine (10 mL) then dried (MgSO 4 ), filtered and evaporated to a foam (900 mg ) which was used without further purification in the next step.
  • HATU 34 mg, 0.89 mmol
  • DIPEA 0.311 mL, 1.79 mmol
  • the reaction mixture was added to ethyl acetate (20 mL), washed with water (10 mL), 2N citric acid (10 mL), saturated aqueous sodium hydrogencarbonate (10 mL) and brine (10 mL), then dried (MgSO 4 ), filtered and evaporated under reduced pressure to a gummy residue.
  • the residue was purified by chromatography on silica, eluting with 50-100% ethyl acetate in hexane, to give a gum which was taken up in 50% ethyl acetate in ether and washed twice with water, brine (10 mL) then dried (MgSO 4 ) to give the desired product as a foam (58 mg).
  • reaction mixture was added to ethyl acetate (50 mL) and water (50 mL), the organic layer washed with brine (50 mL), dried (MgSO 4 ), filtered and the solvent removed in vacuo to give a brown oil.

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Abstract

Compounds of formula (I) wherein R1, HET-1 and HET-2 are as described in the specification, and their salts and pro-drugs, are activators of glucokinase (GLK) and are thereby useful in the treatment of, for example, type 2 diabetes. Processes for preparing compounds of formula (I) are also described.

Description

HETEROARYL BENZAMIDE DERIVATIVES FOR USE AS GLK ACTIVATORS IN THE TREATMENT OF DIABETES
The present invention relates to a group of benzoyl amino heterocyclyl compounds which are useful in the treatment or prevention of a disease or medical condition mediated through glucokinase (GLK or GK), leading to a decreased glucose threshold for insulin secretion. In addition the compounds are predicted to lower blood glucose by increasing hepatic glucose uptake. Such compounds may have utility in the treatment of Type 2 diabetes and obesity. The invention also relates to pharmaceutical compositions comprising said compounds and to methods of treatment of diseases mediated by GLK using said compounds. ' /
In the pancreatic β-cell and liver parenchymal cells the main plasma membrane glucose transporter is GLUT2. Under physiological glucose concentrations the rate at which GLUT2 transports glucose across the membrane is not rate limiting to the overall rate of glucose uptake in these cells. The rate of glucose uptake is limited by the rate of phosphorylation of glucose to glucose-6-phosphate (G-6-P) which is catalysed by glucokinase (GLK) [I]. GLK has a high (6-1OmM) Km for glucose and is not inhibited by physiological concentrations of G-6-P [I]. GLK expression is limited to a few tissues and cell types, most notably pancreatic β-cells and liver cells (hepatocytes) [I]. In these cells GLK activity is rate limiting for glucose utilisation and therefore regulates the extent of glucose induced insulin secretion and hepatic glycogen synthesis. These processes are critical in the maintenance of whole body glucose homeostasis and both are dysfunctional in diabetes [2].
In one sub-type of diabetes, Maturity-Onset Diabetes of the Young Type 2 (MODY-2), the diabetes is caused by GLK loss of function mutations [3, 4]. Hyperglycaemia in MODY-2 patients results from defective glucose utilisation in both the pancreas and liver [5]. Defective glucose utilisation in the pancreas of MODY-2 patients results in a raised threshold for glucose stimulatediiήsulin secretion. Conversely, rare activating mutations of GLK reduce this threshold resulting in familial hyperinsulinism [6, 6a, 7]. In addition to the reduced GLK activity observed in MODY-2 diabetics, hepatic glucokinase activity is also decreased in type 2 diabetics [8]. Importantly, global or liver selective overexpression of GLK prevents or reverses the development of the diabetic phenotype in both dietary and genetic models of the disease [9-12]. Moreover, acute treatment of type 2 diabetics with fructose improves glucose tolerance through stimulation of hepatic glucose utilisation [13]. This effect is believed to be mediated through a fructose induced increase in cytosolic GLK activity in the hepatocyte by the mechanism described below [13]. Hepatic GLK activity is inhibited through association with GLK regulatory protein
(GLKRP). The GLK/GLKRP complex is stabilised by fructose-6-phosphate (F6P) binding to the GLKRP and destabilised by displacement of this sugar phosphate by fructose- 1 -phosphate (FlP). FlP is generated by fructokinase mediated phosphorylation of dietary fructose. Consequently, GLK/GLKRP complex integrity and hepatic GLK activity is regulated in a nutritionally dependent manner as F6P is dominant in the post-absorptive state whereas FlP predominates in the post-prandial state. In contrast to the hepatocyte, the pancreatic β-cell expresses GLK in the absence of GLKRP. Therefore, β-cell GLK activity is regulated extensively by the availability of its substrate, glucose. Small molecules may activate GLK either directly or through destabilising the GLK/GLKRP complex. The former class of compounds are predicted to stimulate glucose utilisation in both the liver and the pancreas whereas the latter are predicted to act selectively in the liver. However, compounds with either profile are predicted to be of therapeutic benefit in treating Type 2 diabetes as this disease is characterised by defective glucose utilisation in both tissues. GLK, GLKRP and the KATP channel are expressed in neurones of the hypothalamus, a region of the brain that is important in the regulation of energy balance and the control of food intake [14-18]. These neurones have been shown to express orectic and anorectic neuropeptides [15, 19, 20] and have been assumed to be the glucose-sensing neurones within the hypothalamus that are either inhibited or excited by changes in ambient glucose concentrations [17, 19, 21, 22]. The ability of these neurones to sense changes in glucose levels is defective in a variety of genetic and experimentally induced models of obesity [23-28]. Intracerebroventricular (icv) infusion of glucose analogues, that are competitive inhibitors of glucokinase, stimulate food intake in lean rats [29, 30]. In contrast, icv infusion of glucose suppresses feeding [31]. Thus, small molecule activators of GLK may decrease food intake and weight gain through central effects on GLK. Therefore, GLK activators may be of therapeutic use in treating eating disorders, including obesity, in addition to diabetes. The hypothalamic effects will be additive or synergistic to the effects of the same compounds acting in the liver and/or pancreas in normalising glucose homeostasis, for the treatment of Type 2 diabetes. Thus the GLK/GLKRP system can be described as a potential "Diabesity" target (of benefit in both Diabetes and Obesity). GLK is also expressed in specific entero-endocrine cells where it is believed to control the glucose sensitive secretion of the incretin peptides GIP (glucose-dependent insulinotropic polypeptide) and GLP-I (Glucagon-Like Peptide- 1) from gut K-cells and L- cells respectively (32, 33, 34). Therefore, small molecule activators of GLK may have additional beneficial effects on insulin secretion, b-cell function and survival and body weight as a consequence of stimulating GIP and GLP-I secretion from these entero- endocrine cells.
In WO00/58293 and WO01/44216 (Roche), a series of benzylcarbamoyl compounds are described as glucokinase activators. The mechanism by which such compounds activate GLK is assessed by measuring the direct effect of such compounds in an assay in which GLK activity is linked to NADH production, which in turn is measured optically - see details of the in vitro assay described hereinafter. Compounds of the present invention may activate GLK directly or may activate GLK by inhibiting the interaction of GLKRP with GLK.
Further GLK activators have been described in WO03/095438 (substituted phenylacetamides, Roche), WO03/055482 (carboxamide and sulphonamide derivatives, Novo Nordisk), WO2004/002481 (arylcarbonyl derivatives, Novo Nordisk), and in WO03/080585 (amino-substituted benzoylaminoheterocycles, Banyu).
Our International application Number: WO03/000267 describes a group of benzoyl amino pyridyl carboxylic acids which are activators of the enzyme glucokinase (GLK). Our International application Number: WO03/015774 describes compounds of the
Formula (A):
Figure imgf000004_0001
(A) wherein R3 is a substituted heterocycle other than a carboxylic acid substituted pyridyl. International application WO2004/076420 (Banyu) describes compounds which are generally a subset of those described in WO03/015774, wherein for example R1 is an (substituted) alkyl ether and R2 is (substituted) phenoxy.
We have surprisingly found a small group of compounds, generally a selected subgroup of those described in WO 03/015774, which have generally superior potency for the GLK enzyme, and more advantageous physical properties, including, for example, higher aqueous solubility, higher permeability, and/or lower plasma protein binding. Consequently, such compounds having a balance of these properties would be expected to display higher plasma free drug levels and superior in vivo efficacy after oral dosing as determined, for example, by activity in Oral Glucose Tolerance Tests (OGTTs). Therefore this group of compounds would be expected to provide superior oral exposure at a lower dose and thereby be particularly suitable for use in the treatment or prevention of a disease or medical condition mediated through GLK. The compounds of the invention may also have superior potency and/or advantageous physical properties (as described above) and/or favourable toxicity profiles and/or favourable metabolic profiles in comparison with other GLK activators known in the art, as well as those described in WO 03/015774.
Thus, according to the first aspect of the invention there is provided a compound of Formula (I):
Figure imgf000005_0001
(I) wherein:
R1 is selected from isopropyl, but-2-yl, cyclopentyl, l,l,l-trifluoroprop-2-yl, 1,3- difluoroprop-2-yl, but-l-yn-3-yl, l-hydroxyprop-2-yl, 2-hydroxybut-3-yl, l-hydroxybut-2- yl, tetrahydrofuryl, tetrahydropyranyl, l-methoxyprop-2-yl, l-methoxybut-2-yl, 2- hydroxyprop-1-yl, 2-methoxyprop-l-yl, 2-hydroxybut-l-yl, 2-methoxybut-l-yl, 1- fiuoromethoxyprop-2-yl, l,l-difluoromethoxyprop-2-yl and l-trifluoromethoxyprop-2-yl; HET-I is a 5- or 6-membered, C-linked heteroaryl ring containing a nitrogen atom in the 2- position and optionally 1 or 2 further ring heteroatoms independently selected from O, N and S; which ring is optionally substituted on any nitrogen atom (provided it is not thereby quaternised) by a substituent selected from R7 and/or on 1 or 2 available carbon atoms by a substituent independently selected from R6;
HET-2 is a 5- or 6- membered heteroaryl ring, containing 1, 2 or 3 ring hetereoatoms independently selected from O, S and N; which ring is substituted on an available carbon atom by a substituent selected from R2, and is optionally further substituted on 1 or 2 available carbon atoms by a substituent independently selected from R3 and/or on an available nitrogen atom (provided it is not thereby quaternised) by a substituent selected
R2 is selected from -C(O)NR4R5 and -SO2NR4R5; R3 is selected from methyl, trifluoromethyl and halo; R4 and R5 together with the nitrogen atom to which they are attached form a 4 to 7 membered saturated or partially unsaturated heterocyclyl ring, optionally containing 1 or 2 further heteroatoms (in addition to the linking N atom) independently selected from O, N and S, wherein a -CH2- group can optionally be replaced by a -C(O)- and wherein a sulphur atom in the ring may optionally be oxidised to a S(O) or S(O)2 group; which ring is optionally substituted on an available carbon atom by 1 or 2 substituents independently selected from R8 and/or on an available nitrogen atom by a substituent selected from R9; or R4 and R5 together with the nitrogen atom to which they are attached form a 6-10 membered bicyclic saturated or partially unsaturated heterocyclyl ring, optionally containing 1 further nitrogen atom (in addition to the linking N atom), wherein a -CH2- group can optionally be replaced by a -C(O)-; which ring is optionally substituted on an available carbon by 1 substituent selected from hydroxy and R3 or on an available nitrogen atom by methyl;
R6 is independently selected from (l-4C)alkyl, halo, hydroxy(l-4C)alkyl, (l-4C)alkoxy(l- 4C)alkyl, (l-4C)alkylS(O)p(l-4C)alkyl, amino(l-4C)alkyl, (l-4C)alkylamino(l-4C)alkyl and di(l-4C)alkylamino(l-4C)alkyl; R7 is independently selected from (l-4C)alkyl, halo(l-4C)alkyl, dihalo(l-4C)alkyl, trihalo(l-4C)alkyl, hydroxy(l-4C)alkyl, (l-4C)alkoxy(l-4C)alkyl, (l-4C)alkylS(0)p(l- 4C)alkyl, amino(l-4C)alkyl, (l-4C)alkylamino(l-4C)alkyl and di(l-4C)alkylamino(l-
4C)alkyl;
R8 is selected from hydroxy, (l-4C)alkoxy, (l-4C)alkyl, aminocarbonyl, (1-
4C)alkylaminocarbonyl, di(l-4C)alkylaminocarbonyl, (l-4C)alkylamino, di(l- 4C)alkylamino, (l-4C)alkoxy(l-4C)alkyl, hydroxy(l-4C)alkyl and -S(0)p(l-4C)alkyl;
R9 is selected from (l-4C)alkyl, -C(O)(I -4C)alkyl, aminocarbonyl, (1-
4C)alkylaminocarbonyl, di( 1 -4C)alkylaminocarbonyl, ( 1 -4C)alkoxy( 1 -4C)alkyl, hydroxy(l-4C)alkyl and -S(0)p(l-4C)alkyl;
R10 is selected from (l-4C)alkyl, (3-6C)cycloalkyl, hydroxy(l-4C)alkyl, (l-4C)alkoxy(l- 4C)alkyl, -C(O)(I -4C)alkyl, benzyl, and (l-4C)alkylsulfonyl; p is (independently at each occurrence) O, 1 or 2; or a salt thereof.
In another aspect of the invention there is provided a compound of formula (I) as hereinbefore defined wherein R1 is selected from isopropyl, but-2-yl, 1,1,1-trifluoroprop- 2-yl, l,3-difluoroprop-2-yl, but-l-yn-3-yl, l-hydroxyprop-2-yl, 2-hydroxybut-3-yl, 1- hydroxybut-2-yl, tetrahydrofuryl, tetrahydropyranyl, l-methoxyprop-2-yl, 1-methoxybut-
2-yl, l-fluoromethoxyprop-2-yl, l,l-difluoromethoxyprop-2-yl and 1- trifluoromethoxyprop-2-yl; or a salt thereof. In another aspect of the invention there is provided a compound of formula (I) as hereinbefore defined wherein R1 is selected from isopropyl, but-2-yl, 1,1,1-trifluoroprop-
2-yl, l,3-difluoroprop-2-yl, but-l-yn-3-yl, l-hydroxyprop-2-yl, 2-hydroxybut-3-yl, 1- hydroxybut-2-yl, tetrahydrofuryl, tetrahydropyranyl, l-methoxyprop-2-yl, 1-methoxybut-
2-yl, 2-hydroxyprop-l-yl, 2-methoxyprop-l-yl, 2-hydroxybut-l-yl, 2-methoxybut-l-yl, 1- fluoromethoxyprop-2-yl, l,l-difluoromethoxyprop-2-yl and l-trifluoromethoxyprop-2-yl; or a salt thereof.
In another aspect of the invention there is provided a compound of formula (I) as hereinbefore defined wherein R3 is halo; or a salt thereof.
In another aspect of the invention there is provided a compound of formula (I) as hereinbefore defined wherein R7 is independently selected from (l-4C)alkyl, hydroxy(l-
4C)alkyl, (l-4C)alkoxy(l-4C)alkyl, (l-4C)alkylS(O)p(l-4C)alkyl, amino(l-4C)alkyl, (1-
4C)alkylamino(l-4C)alkyl and di(l-4C)alkylamino(l-4C)alkyl; or a salt thereof. It will be appreciated that, where definitions of heterocylyl groups HET-I and HET-2 encompass heteroaryl rings which may be substituted on nitrogen, such substitution may not result in charged quaternary nitrogen atoms, removal of aromaticity of the ring or unstable structures. It will be appreciated that the definitions of HET-I and HET-2 are not intended to include any O-O, O-S or S-S bonds. It will be appreciated that the definitions of HET-I and HET-2 are not intended to include unstable structures.
It will be understood that any single carbon atom in HET-I may only be substituted by one group R6 in order to maintain aromaticity of the ring. Up to two different carbon atoms in a HET-I ring may be substituted by an R6 group, each of which may be the same or different, provided the structure thereby formed is stable and aromatic.
It will be understood that any single carbon atom in HET-2 may only be substituted by one group R3 in order to maintain aromaticity of the ring. Up to two different carbon atoms in a HET-2 ring may be substituted by an R3 group, each of which may be the same or different, provided the structure thereby formed is stable and aromatic. It will be understood that R8 can be present on any or all available carbon atoms in the heterocyclic ring formed by NR4R5; each carbon atom can be substituted with 1 or 2 R8 groups which may be the same or different, provided the structure thereby formed is stable (so, for example, it is not intended to cover gem-dihydroxy substitution).
It will be understood that where a compound of the formula (I) contains more than one group R5, they may be the same or different.
It will be understood that where a compound of the formula (I) contains more than one group R3, they may be the same or different.
A similar convention applies for all other groups and substituents on a compound of formula (I) as hereinbefore defined. Compounds of Formula (I) may form salts which are within the ambit of the invention. Pharmaceutically acceptable salts are preferred although other salts may be useful in, for example, isolating or purifying compounds.
In another aspect, the invention relates to compounds of formula (I) as hereinabove defined or to a pharmaceutically acceptable salt. In this specification the generic term "alkyl" includes both straight-chain and branched-chain alkyl groups. However references to individual alkyl groups such as "propyl" are specific for the straight chain version only and references to individual branched-chain alkyl groups such as t-butyl are specific for the branched chain version only. For example, "(l-4C)alkyl" includes methyl, ethyl, propyl, isopropyl and t-butyl. An analogous convention applies to other generic terms.
For the avoidance of doubt, reference to the group HET-I containing a nitrogen in the 2-position, is intended to refer to the 2-position relative to the amide nitrogen atom to which the group is attached. For example, HET-I encompasses but is not limited to the following structures:
Suitable examples of HET-I as a 5- or 6-membered, C-linked heteroaryl ring as hereinbefore defined, include thiazolyl, isothiazolyl, thiadiazolyl, pyridyl, pyrazinyl, pyridazinyl, pyrazolyl, imidazolyl, pyrimidinyl, oxazolyl, isoxazolyl, oxadiazolyl and triazolyl.
Suitable examples of HET-2 include thienyl, furyl, thiazolyl, isothiazolyl, thiadiazolyl, pyridyl, pyrazinyl, pyridazinyl, pyrazolyl, imidazolyl, pyrimidinyl, oxazolyl, isoxazolyl, oxadiazolyl and triazolyl. Further suitable examples of HET-2 include aromatic heterocycles where a ring nitrogen or sulfur atom has been oxidised but aromaticity has been preserved, for example a pyridine N-oxide. Further suitable examples of HET-2 include thiazolyl, pyridyl, pyrazinyl, pyridazinyl and pyrimidinyl. Suitable examples for a 4-7 membered ring formed by R4 and R5 together with the nitrogen to which they are attached, as hereinbefore defined, include morpholino, piperidinyl, piperazinyl, pyrrolidinyl, azetidinyl, homopiperazinyl, homo-morpholino, homo-thiomorpholino (and versions thereof wherein the sulfur is oxidised to an SO or S(O)2 group) and homo-piperidinyl. A further suitable example is thiomorpholino (and versions thereof wherein the sulfur is oxidised to an SO or S(O)2 group).
Suitable examples for a 6-10 membered bicyclic heterocyclic ring formed by R4 and R5 together with the nitrogen to which they are attached, as hereinbefore defined, are bicyclic saturated or partially unsaturated heterocyclyl ring such as those illustrated by the structures shown below (wherein the dotted line indicates the point of attachment to the rest of the molecule and wherein R represents the optional substituents for carbon or nitrogen defined hereinbefore):
Figure imgf000010_0001
Figure imgf000010_0002
Figure imgf000010_0003
[2,2,2] [4,1,0] [4,2,0]
Figure imgf000010_0004
Figure imgf000010_0005
[3,2,0] [3,1 ,0]
Figure imgf000010_0006
,1, [3,1,0] I1 '1'1!
In particular such a ring system is a [2,2,1] system such as
Figure imgf000010_0007
(7-azabicyclo[2.2.1]hept-7-yl).
In another embodiment, such a ring system is a [2.1.1] system such as
Figure imgf000011_0001
(2-azabicyclo[2.1. l]hex-2-yl).
Examples of (l-4C)alkyI include methyl, ethyl, propyl, isopropyl, butyl and tert- butyl; examples of (3-6C)cycloalkyl include cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl; examples of halo include fluoro, chloro, bromo and iodo; examples of halo(l- 4C)aIkyl include fluoromethyl, chloromethyl, fluoroethyl, chloroethyl, fluoropropyl and fluorobutyl; examples of dihalo(l-4C)alkyl include difluoromethyl, l,l-difluoroeth-2-yl, l,2-difluoroeth-2-yl, l,l-dichloroeth-2-yl, l,2-dichloroeth-2-yl, and l,l-difluoroprop-3-yl; examples of trihaIo(l-4C)aIkyl include trifluoromethyl and l,l,l-trifluoroeth-2-yl; examples of hydroxy(l-4C)alkyl include hydroxymethyl, 1-hydroxyethyl, 2- hydroxyethyl, 2-hydroxypropyl, 3-hydroxypropyl, 1-hydroxyisopropyl and 4- hydroxybutyl; examples of (l-4C)alkoxy(l-4C)alkyl include methoxymethyl, ethoxymethyl, tert-butoxymethyl, 2-methoxyethyl, 2-ethoxyethyl, methoxypropyl, 2- methoxypropyl and methoxybutyl; example of (l-4C)aIkoxy include methoxy, ehtoxy, propoxy, isopropxy, butoxy and tert-butoxy; examples of (l-4C)alkylS(O)p(l-4C)alkyl (where p is 0, 1 or 2) include methylsulfinylmethyl, ethylsulfmylmethyl, ethylsulfinylethyl, methylsulfinylpropyl, methylsulfϊnylbutyl, methylsulfonyhnethyl, ethylsulfonylmethyl, ethylsulfonylethyl, methylsulfonylpropyl, methylsulfonylbutyl, methylthiomethyl, ethylthiomethyl, ethylthioethyl, methylthiopropyl, and methylthiobutyl; examples of (l-4C)alkylsulfonyl include methylsulfonyl, ethylsulfonyl, propylsulfonyl, isopropylsulfonyl and tert-butylsulfonyl; examples of-S(0)p(l-4C)alkyl include (1- 4C)alkylsulfonyl, methylsulfinyl, ethylsulfϊnyl, propylsulfinyl, isopropylsulfϊnyl, tert- butylsulfinyl, methylthio, ethylthio, propylthio, isopropylthio and tert-butylthio; examples of amino(l-4C)aIkyl include aminomethyl, aminoethyl, 2-aminopropyl, 3-aminopropyl, 1- aminoisopropyl and 4-aminobutyl; examples of (l-4C)aIkylamino(l-4C)aIkyl include (N- methyl)aminomethyl, (N-ethyl)aminomethyl, l-((N-methyl)amino)ethyl, 2-((N- methyl)amino)ethyl, (N-ethyl)aminoethyl, (N-methyl)aminopropyl, and 4-((N- methyl)amino)butyl; examples of di(l-4C)aIkyIamino(l-4C)aIkyl include dimethylaminomethyl, methyl(ethyl)aminomethyl, methyl(ethyl)aminoethyl, (N9N- diethyl)aminoethyl, (N,N-dimethyl)aminopropyl and (N,N-dimethyl)aminobutyl; examples of-C(O)(l-4C)a!kyl and (l-4C)alkylcarbonyl include methylcarbonyl, ethylcarbonyl, propylcarbonyl and tert-butyl carbonyl; examples of (l-4C)alkyIamino include methylamino, ethylamino, propylamino, isopropylamino, butylamino and tert-butylamino; examples of di(l-4C)alkylamino include dimethylamino, diethylamino, N-methyl-N- ethylamino, dipropylamino, N-isopropyl-N-methyamino and dibutylamino; examples of (l-4C)alkylaminocarbonyl include methylaminocarbonyl, ethylaminocarbonyl, propylaminocarbonyl, isopropylaminocarbonyl, butylaminocarbonyl and tert- butylaminocarbonyl; examples of di(l-4C)aIkylaminocarbonyl include dimethylaminocarbonyl, diethylaminocarbonyl, N-methyl-N-ethylaminocarbonyl, dipropylaminocarbonyl, N-isopropyl-N-methyaminocarbonyl and dibutylaminocarbonyl.
It is to be understood that, insofar as certain of the compounds of Formula (I) defined above may exist in optically active or racemic forms by virtue of one or more asymmetric carbon atoms, the invention includes in its definition any such optically active or racemic form which possesses the property of stimulating GLK directly or inhibiting the GLK/GLKRP interaction. The synthesis of optically active forms may be carried out by standard techniques of organic chemistry well known in the art, for example by synthesis from optically active starting materials or by resolution of a racemic form. It is also to be understood that certain compounds may exist in tautomeric forms and that the invention also relates to any and all tautomeric forms of the compounds of the invention which activate GLK.
It is also to be understood that certain compounds of the formula (1) and salts thereof can exist in solvated as well as unsolvated forms such as, for example, hydrated forms. It is to be understood that the invention encompasses all such solvated forms which activate GLK.
In another aspect, the invention relates to compounds of formula (I) as hereinabove defined or to a pro-drug thereof. Suitable examples of pro-drugs of compounds of formula (I) are in- vivo hydrolysable esters of compounds of formula (I). Therefore in another aspect, the invention relates to compounds of formula (I) as hereinabove defined or to an in- vivo hydrolysable ester thereof.
In one embodiment of the invention are provided compounds of formula (I), in an alternative embodiment are provided pharmaceutically-acceptable salts of compounds of formula (I), in a further alternative embodiment are provided in-vivo hydrolysable esters of compounds of formula (I), and in a further alternative embodiment are provided pharmaceutically-acceptable salts of in-vivo hydrolysable esters of compounds of formula (I).
The compounds of the invention may be administered in the form of a pro-drug. A pro-drug is a bioprecursor or pharmaceutically acceptable compound being degradable in the body to produce a compound of the invention (such as an ester or amide of a compound of the invention, particularly an in-vivo hydrolysable ester). Various forms of prodrugs are known in the art. For examples of such prodrug derivatives, see: a) Design of Prodrugs, edited by H. Bundgaard, (Elsevier, 1985) and Methods in En∑ymology, Vol. 42, p. 309-396, edited by K. Widder, et al. (Academic Press, 1985); b) A Textbook of Drug Design and Development, edited by Krogsgaard-Larsen; c) H. Bundgaard, Chapter 5 "Design and Application of Prodrugs", by H.
Bundgaard p. 113-191 (1991); d) H. Bundgaard, Advanced Drug Delivery Reviews, 8, 1-38 (1992); e) H. Bundgaard, et al., Journal of Pharmaceutical Sciences, 77, 285 (1988); and f) N. Kakeya, et al, Chem Pharm Bull, 32, 692 (1984). The contents of the above cited documents are incorporated herein by reference. Examples of pro-drugs are as follows. An in-vivo hydrolysable ester of a compound of the invention containing a carboxy or a hydroxy group is, for example, a pharmaceutically-acceptable ester which is hydrolysed in the human or animal body to produce the parent acid or alcohol. Suitable pharmaceutically-acceptable esters for carboxy include Ci to Cβalkoxymethyl esters for example methoxymethyl, C1 to C βalkanoyloxymethyl esters for example pivaloyloxymethyl, phthalidyl esters, C3 to Cscycloalkoxycarbonyloxyd to C6alkyl esters for example l-cyclohexylcarbonyloxyethyl; l,3-dioxolen-2-onylmethyl esters, for example 5-methyl-l,3-dioxolen-2-onylmethyl; and (l-6C)alkoxycarbonyloxyethyl esters. An in-vivo hydrolysable ester of a compound of the invention containing a hydroxy group includes inorganic esters such as phosphate esters (including phosphoramidic cyclic esters) and α-acyloxyalkyl ethers and related compounds which as a result of the in- vivo hydrolysis of the ester breakdown to give the parent hydroxy group/s. Examples of α-acyloxyalkyl ethers include acetoxymethoxy and 2,2-dimethylpropionyloxy-methoxy. A selection of in- vivo hydrolysable ester forming groups for hydroxy include alkanoyl, benzoyl, phenylacetyl and substituted benzoyl and phenylacetyl, alkoxycarbonyl (to give alkyl carbonate esters), dialkylcarbamoyl and N- (dialkylaminoethyl)-N-alkylcarbamoyl (to give carbamates), dialkylaminoacetyl and carboxyacetyl.
A suitable pharmaceutically-acceptable salt of a compound of the invention is, for example, an acid-addition salt of a compound of the invention which is sufficiently basic, for example, an acid-addition salt with, for example, an inorganic or organic acid, for example hydrochloric, hydrobromic, sulphuric, phosphoric, trifluoroacetic, citric or maleic acid. In addition a suitable pharmaceutically-acceptable salt of a benzoxazinone derivative of the invention which is sufficiently acidic is an alkali metal salt, for example a sodium or potassium salt, an alkaline earth metal salt, for example a calcium or magnesium salt, an ammonium salt or a salt with an organic base which affords a physiologically-acceptable cation, for example a salt with methylamine, dimethylamine, trimethylamine, piperidine, morpholine or tris-(2-hydroxyethyl)amine.
In a further aspect of the invention there is provided a compound of formula (I) which is a compound of formula (IA), or a salt thereof:
Figure imgf000014_0001
(IA) wherein each of X1, X2 and X3 is independently selected from CH, N, S and O;
X4 is absent (to make a 5-membered ring) or is selected from CH, N, O and S; provided that at least one of X1, X2, X3 and X4 is CH and provided that there are no O-O, O-S or S-S bonds within the ring;
R3, if present, is selected from methyl, trifluoromethyl and halo;
R1, R2 and HET-I are as defined for a compound of formula (I). It will be understood that the dotted circle inside the ring containing X1 to X4 (that is, the HET-2 ring) is intended to indicate that the ring is aromatic, although the precise number and position of the double bonds will be dependent on the nature of X1 to X4.
References herein to a compound of formula (I) should generally be understood to apply equally to a compound of formula (IA)5 whether explicitly stated or not, unless the context indicates otherwise.
Particular examples of compounds of formula (I) and (IA) include compounds of formulae (IB), (IC) and/or (ID):
Figure imgf000015_0001
(IB) (IC)
Figure imgf000015_0002
(ID) wherein R1, R2 and HET-I are as defined for a compound of formula (I). Preferred values of each variable group are as follows. Such values may be used where appropriate with any of the values, definitions, claims, aspects or embodiments defined hereinbefore or hereinafter. In particular, each may be used as an individual limitation on the broadest definition of formula (I) or (IA). Further, each of the following values may be used in combination with one or more of the other following values to limit the broadest defintion of formula (I) or (IA) or to limit any narrower definitions of formula (I) or (IA) in any of the aspects hereinbefore or hereinafter. Where appropriate each of the following values may also be used to limit any definition within formulae (IB), (IC) and/or (ID). (1) R1 is of sub-formula X:
Figure imgf000016_0001
(X) wherein Rx is selected from methyl, ethyl, trifluoromethyl, ethynyl, hydroxymethyl, hydroxyethyl, methoxymethyl, fluoromethoxymethyl, difluoromethoxymethyl and trifluoromethoxymethyl; preferably Rx is selected from methyl, ethyl, trifluoromethyl, ethynyl, hydroxymethyl, hydroxyethyl, methoxymethyl, fluoromethoxymethyl and difluoromethoxymethyl
(2) R1 is of sub-formula Y:
Figure imgf000016_0002
(Y) wherein Ry is selected from hydroxymethyl and methoxymethyl
(3) R1 is l-hydroxyprop-2-yl and the configuration is preferably (S), that is R!-O- is:
Figure imgf000016_0003
(4) R1 is l-methoxyprop-2-yl and the configuration is preferably (S), that is R^O- is:
Figure imgf000016_0004
(5) R1 is selected from isopropyl, l,3-difluoroprop-2-yl, but-l-yn-3-yl, l-hydroxyprop-2- yl, hydroxybut-3-yl and l-methoxyprop-2-yl
(6) R1 is l,l,l-trifluoroprop-2-yl, l-fϊuoromethoxyprop-2-yl, l,l-difluoromethoxyprop-2- yl or l-trifluoromethoxyprop-2-yl
(7) R1 is l-fluoromethoxyprop-2-yl, l,l-difluoromethoxyprop-2-yl or 1- trifluoromethoxyprop-2-yl, particularly l-fluoromethoxyprop-2-yl or 1,1- difluoromethoxyprop-2-yl
(8) R1 is l,l-difluoromethoxyprop-2-yl, particularly with the stereochemistry:
Figure imgf000017_0001
(9) R1 is tetrahydrofuryl or tetrahydropyranyl
(10) R1 is tetrahydrofuryl in the (S) configuration, that is:
Figure imgf000017_0002
(11) R1 is tetrahydrofuryl in the (R) configuration, that is:
Figure imgf000017_0003
(12) R1 is 4-tetrahydropyranyl:
Figure imgf000017_0004
(13) R1 is 2-hydroxy-but-3-yl and the configuration is preferably such that RJ-O- is:
Figure imgf000017_0005
(14) R1 is l-hydroxybut-2-yl or l-methoxybut-2-yl
(15) R1 is selected from isopropyl, but-2-yl, l,l,l-trifluoroprop-2-yl, l,3-difluoroprop-2- yl, but-l-yn-3-yl, l-hydroxyprop-2-yl, 2-hydroxybut-3-yl, tetrahydrofuryl, tetrahydropyranyl, l-methoxyprop-2-yl, l-fluoromethoxyprop-2-yl, 1,1- difluoromethoxyprop-2-yl and l-trifluoromethoxyprop-2-yl
(16) R1 is selected from 2-hydroxyprop-l-yl, 2-methoxyprop-l-yl, 2-hydroxybut-l-yl and 2-methoxybut- 1 -yl
(17) R1 is selected from isopropyl, l,3-difluoroprop-2-yl, but-2-yl, l-hydroxyprop-2-yl, 2- hydroxybut-3-yl, 1,1 -difluoromethoxyprop-2-yl, tetrahydrofuryl, l-hydroxybut-2-yl and 1- methoxyprop-2-yl (18) R1 is selected from isopropyl, cyclopentyl, l,3-difluoroprop-2-yl, but-2-yl, 1- hydroxyprop-2-yl, 2-hydroxybut-3-yl, l,l-difluoromethoxyprop-2-yl, tetxahydrofuryl, tetrahydropyranyl, l-hydroxybut-2-yl and l-methoxyprop-2-yl
(19) R1 is selected from tetrahydrofuryl, l-difluoromethoxyprop-2-yl, l,3-difluoroprop-2- yl, and 2-hydroxybut-3-yl
(20) R1 is selected from isopropyl, tetrahydrofuryl, l-hydroxyprop-2-yl and 1- methoxyprop-2-yl
(21) R1 is selected from l-hydroxyprop-2-yl and l-methoxyprop-2-yl, for example (2S)- 1- hydroxyprop-2-yl and (25)- l-methoxyprop-2-yl (22) R1 is selected from isopropyl, but-2-yl, cyclopentyl, l,l,l-trifluoroprop-2-yl, 1,3- difluoroprop-2-yl, but-l-yn-3-yl, l-hydroxyprop-2-yl, 2-hydroxybut-3-yl, l-hydroxybut-2- yl, tetrahydrofuryl, tetrahydropyranyl, l-methoxyprop-2-yl, l-methoxybut-2-yl, 2- hydroxyprop-1-yl, 2-methoxyprop-l-yl, 2-hydroxybut-l-yl, 2-methoxybut-l-yl, 1- fluoromethoxyprop-2-yl and 1, l-difluoromethoxyprop-2-yl (23) R1 is selected from isopropyl, but-2-yl, cyclopentyl, l,l,l-trifluoroprop-2-yl, 1,3- difluoroprop-2-yl, but-l-yn-3-yl, l-hydroxyprop-2-yl, 2-hydroxybut-3-yl, l-hydroxybut-2- yl, tetrahydrofuryl, tetrahydropyranyl, l-methoxyprop-2-yl, l-methoxybut-2-yl, 2- hydroxyprop-1-yl, 2-methoxyprop-l-yl, 2-hydroxybut-l-yl, 2-methoxybut-l-yl and 1,1- difluoromethoxyprop-2-yl (24) HET-I is a 5-membered heteroaryl ring
(25) HET-I is a 6-membered heteroaryl ring
(26) HET-I is substituted with 1 or 2 substituents independently selected from R6
(27) HET-I is substituted with 1 substituent selected from R6
(28) HET-I is substituted with 1 substituent selected from R7 (29) HET-I is unsubstituted
(30) HET-I is selected from thiazolyl, isothiazolyl, thiadiazolyl, pyridyl, pyrazinyl, pyridazinyl, pyrazolyl, imidazolyl, pyrimidinyl, oxazolyl, isoxazolyl, oxadiazolyl, and triazolyl
(31) HET-I is selected from thiazolyl, isothiazolyl, thiadiazolyl, pyrazolyl, imidazolyl, oxazolyl, isoxazolyl and oxadiazolyl
(32) HET-I is selected from pyridyl, pyrazinyl, pyridazinyl and pyrimidinyl
(33) HET-I is selected from thiazolyl, pyrazolyl and oxazolyl (34) HET-I is selected from thiadiazolyl and oxadiazolyl
(35) HET-I is selected from 1,3,4-thiadiazolyl and 1,3,4-oxadiazolyl
(36) HET-I is selected from 1,2,4-oxadiazolyl and 1,2,4-oxadiazolyl
(37) HET-I is pyrazolyl, particularly N-methylpyrazolyl (38) HET-I is pyrazinyl
(39) HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl and pyrazinyl
(40) HET-I is selected from thiazolyl, pyrazolyl, and thiadiazolyl, optionally substituted with (l-4C)alkyl
(41) HET-I is pyrazolyl, particularly N-methylpyrazolyl (42) HET-I is pyrazolyl (optionally substituted with ethyl, isopropyl or 1 or 2 methyl), thiazolyl (optionally substituted with methyl), pyrazinyl (optionally substituted with methyl), pyridyl (optionally substituted by fluoro), isoxazolyl (optionally substituted with methyl) and thiadiazolyl (optionally substituted with methyl)
(43) HET-I is pyrazolyl (optionally substituted with ethyl, isopropyl, difluoromethyl, or 1 or 2 methyl), thiazolyl (optionally substituted with methyl), pyrazinyl (optionally substituted with methyl), pyridyl (optionally substituted by fluoro), isoxazolyl (optionally substituted with methyl) and thiadiazolyl (optionally substituted with methyl)
(44) HET-I is selected from pyrazinyl (optionally substituted with methyl), pyrazolyl (optionally substituted on carbon by methyl), methylthiadiazolyl (particularly 1,2,4- thiadiazol-5-yl, more particularly 3-methyl- 1 ,2,4-thiadiazol-5-yl), thiazolyl (optionally substituted with methyl), pyridyl (optionally substituted by fluoro) and isoxazolyl
(45) HET-I is N-difluoromethylpyrazolyl
(46) HET-I is 5-methylpyrazin-2-yl
(47) HET-I is pyrazolyl (optionally substituted with ethyl, isopropyl, difluoromethyl, or 1 or 2 methyl), thiazolyl (optionally substituted with methyl), pyrazinyl (optionally substituted with methyl), pyridyl (optionally substituted by fluoro), isoxazolyl (optionally substituted with methyl) and thiadiazolyl (optionally substituted with methyl); and R1 is selected from isopropyl, cyclopentyl, l,3-difluoroproρ-2-yl, but-2-yl, 1- hydroxyprop-2-yl, 2-hydroxybut-3-yl, l,l-difluoromethoxyprop-2-yl, tetrahydroruryl, tetrahydropyranyl, l-hydroxybut-2-yl and l-methoxyρrop-2-yl; when HET-I is pyrazolyl unsubstituted on nitrogen (ie NH-pyrazolyl), particularly R1 is selected from 1- methoxyprop-2-yl, isopropyl, and tetrahydrofuryl (48) HET-I is pyrazolyl (optionally substituted on carbon with methyl), thiazolyl (optionally substituted with methyl), pyrazinyl (optionally substituted with methyl), pyridyl (optionally substituted by fluoro), isoxazolyl and methylthiadiazolyl (particularly 3-methyl- 1 ,2,4-thiadiazol-5-yl); R1 is selected from isopropyl, cyclopentyl, l,3-difluoroprop-2-yl, but-2-yl, 1- hydroxyprop-2-yl, 2-hydroxybut-3-yl, l,l-difluoromethoxyprop-2-yl, tetrahydrofuryl, tetrahydropyranyl, l-hydroxybut-2-yl and l-methoxyprop-2-yl; when HET-I is pyrazolyl, particularly R1 is selected from l-methoxyprop-2-yl, isopropyl, and tetrahydrofuryl
(49) HET-I is methylpyrazinyl and R1 is selected from l-hydroxyprop-2-yl and 1- methoxyprop-2-yl, for example (2S)- l-hydroxyprop-2-yl and (2S)- l-methoxyprop-2-yl
(50) HET-I is pyrazolyl and R1 is selected from isopropyl, tetrahydrofuryl and 1- methoxyprop-2-yl, for example (2S)- l-methoxyprop-2-yl
(51) R6 is selected from (l-4C)alkyl, halo, hydroxy(l-4C)alkyl and di(l-4C)alkylamino(l- 4C)alkyl (52) R6 is selected from methyl, ethyl, bromo, chloro, fluoro, hydroxymethyl, methoxymethyl, aminomethyl, N-methylaminomethyl, dimethylaminomethyl (53) R6 is selected from (l-4C)alkyl, halo, hydroxy(l-4C)alkyl, (l-4C)alkoxy(l-4C)alkyl, (l-4C)alkylS(O)p(l-4C)alkyl, amino(l-4C)alkyl, (l-4C)alkylamino(l-4C)alkyl, and di(l- 4C)alkylamino( 1 -4C)alkyl (54) R6 is selected from methyl, ethyl, bromo, chloro, fluoro, hydroxymethyl and methoxymethyl
(55) R6 is selected from methyl, ethyl, chloro and fluoro
(56) R6 is methyl or fluoro, preferably methyl
(57) R6 is selected from methyl, ethyl, bromo, chloro, fluoro, aminomethyl, N- methylaminomethyl, dimethylaminomethyl, hydroxymethyl and methoxymethyl
(58) R6 is selected from methyl, ethyl, aminomethyl, N-methylaminomethyl, dimethylaminomethyl, hydroxymethyl and methoxymethyl
(59) R6 is selected from methyl, ethyl, isopropyl and methoxymethyl
(60) when 2 substituents R6 are present, both are selected from methyl, ethyl, bromo, chloro and fluoro; preferably both are methyl
(61) R6 is selected from (l-4C)alkylS(O)p(l-4C)alkyl, (l-4C)alkylamino(l-4C)alkyl and di(l-4C)alkylamino(l-4C)alkyl (62) R7 is selected from (l-4C)alkyl, hydroxy(l-4C)alkyl and di(l-4C)alkylamino(l- 4C)alkyl
(63) R7 is selected from methyl, ethyl, hydroxymethyl, methoxymethyl, aminomethyl, N-methylaminomethyl, dimethylaminomethyl (64) R7 is selected from (l-4C)alkyl, hydroxy(l-4C)alkyl, (l-4C)alkoxy(l-4C)alkyl, (1- 4C)alkylS(O)p(l-4C)alkyl, amino(l-4C)alkyl, (l-4C)alkylamino(l-4C)alkyl, and di(l- 4C)alkylamino(l-4C)alkyl
(65) R7 is selected from methyl, ethyl, aminomethyl, N-methylaminomethyl, and dimethylaminomethyl (66) R7 is selected from methyl, ethyl, hydroxymethyl and methoxymethyl
(67) R7 is selected from methyl, isopropyl and ethyl
(68) R7 is selected from methyl, isopropyl, difluoromethyl and ethyl
(69) R7 is selected from isopropyl and difluoromethyl, particularly difluoromethyl
(70) R7 is selected from methyl and ethyl (71) R7 is methyl
(72) R7 is selected from methyl, ethyl, aminomethyl, N-methylaminomethyl, dimethylaminomethyl, hydroxymethyl and methoxymethyl
(73) R7 is selected from methyl, ethyl, isopropyl and methoxymethyl
(74) when R7 is selected from halo(l-4C)alkyl, dihalo(l-4C)alkyl and trihalo(l-4C)alkyl, each halo is selected from chloro and fluoro, and is in particular fluoro.
(75) when R7 is selected from halo(l-4C)alkyl, dihalo(l-4C)alkyl and trihalo(l-4C)alkyl, R7 is particularly selected from fluoromethyl, difluoroethyl, difluoromethyl and trifluoromethyl
(76) HET-2 is a 5-membered ring (77) HET-2 is a 6-membered ring
(78) HET-2 is selected from thienyl, furyl, thiazolyl, isothiazolyl, thiadiazolyl, pyridyl, pyrazinyl, pyridazinyl, pyrazolyl, imidazolyl, pyrimidinyl, oxazolyl, isoxazolyl and oxadiazolyl
(79) HET-2 is selected from thienyl, furyl, thiadiazolyl, pyridyl, pyrazinyl, pyridazinyl, pyrazolyl, imidazolyl, pyrimidinyl and oxadiazolyl
(80) HET-2 is selected from pyridyl, pyrazinyl, thiazolyl and thienyl
(81) HET-2 is selected from pyridyl, pyrazinyl and thiazolyl (82) HET-2 is selected from pyridyl, pyrazinyl, pyridazinyl and thiazolyl
(83) HET-2 is selected from pyridyl and pyrazinyl
(84) HET-2 is pyrazinyl
(85) HET-2 is substituted with a substituent selected from R3 (86) HET-2 has one nitrogen substituent selected from R10
(87) R3 is chloro or fluoro
(88) R3 is chloro
(89) R3 is fluoro
(90) R3 is chloro or methyl (91) R3 is fluoro, chloro or methyl
(92) R2 is -C(O)NR4R5
(93) R2 is -SO2NR4R5
(94) R4 and R5 together with the nitrogen atom to which they are attached form a 4 membered ring (95) R4 and R5 together with the nitrogen atom to which they are attached form a 5 membered ring
(96) R4 and R5 together with the nitrogen atom to which they are attached form a 6 membered ring
(97) R4 and R5 together with the nitrogen atom to which they are attached form a 7 membered ring
(98) R4 and R5 together with the nitrogen atom to which they are attached form a fully saturated ring
(99) R4 and R5 together with the nitrogen atom to which they are attached form a ring selected from morpholino, piperidinyl, piperazinyl, pyrrolidinyl and azetidinyl (100) R4 and R5 together with the nitrogen atom to which they are attached form a ring selected from pyrrolidinyl, morpholino and azetidinyl
(101) R4 and R5 together with the nitrogen atom to which they are attached form a ring selected from 7-azabicyclo[2.2.1]hept-7-yl, pyrrolidinyl, morpholino and azetidinyl
(102) R4 and R5 together with the nitrogen atom to which they are attached form an azetidinyl ring
(103) R4 and R5 together with the nitrogen atom to which they are attached form an azetidinyl or pyrrolidinyl ring (104) R4 and R5 together with the nitrogen atom to which they are attached form an unsubstituted ring
(105) R4 and R5 together with the nitrogen atom to which they are attached form an ring mono-substituted either with a substituent R8 or with a substituent R9 (106) R4 and R5 together with the nitrogen atom to which they are attached form a 6-10 membered bicyclic saturated or partially unsaturated ring
(107) R8 is selected from hydroxy, (l-4C)alkoxy, (l-4C)alkyl
(108) R8 is selected from hydroxy, methoxy and methyl
(109) R9 is selected from (l-4C)alkyl and -C(O)(I -4C)alkyl (110) R2 is azetidinylcarbonyl or pyrrolidinylcarbonyl, preferably azetidinylcarbonyl (109) R10 is (l-4C)alkyl
(111) R10 is (3-6C)cycloalkyl
(112) R10 is hydroxy(l-4C)alkyl or (l-4C)alkoxy(l-4C)alkyl
(113) R10 is -C(O)(l-4C)alkyl (114) R10 is benzyl
(115) R10 is (l-4C)alkylsulfonyl
(116) R10 is (l-4C)alkyl or benzyl
According to a further feature of the invention there is provided the following preferred groups of compounds of the invention:
In one aspect of the invention there is provided a compound of formula (I) or (IA) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from isopropyl, but-2-yl, l,l,l-trifluoroprop-2-yl, l,3-difluoroprop-2-yl, but-l-yn-3-yl, l-hydroxyprop-2-yl, 2-hydroxybut-3-yl, tetrahydrofuryl, tetrahydropyranyl, l-methoxyprop-2-yl, l-fluoromethoxyprop-2-yl and l,l-difluoromethoxyprop-2-yl;
HET-I is a 5- or 6-membered, C-linked heteroaryl ring containing a nitrogen atom in the 2- position and optionally 1 or 2 further ring heteroatoms independently selected from O, N and S; which ring is optionally substituted on any nitrogen atom (provided it is not thereby quaternised) by a methyl or ethyl group and/or on 1 or 2 available carbon atoms by a methyl or ethyl group;
HET-2 is a 5- or 6- membered heteroaryl ring, containing 1, 2 or 3 ring hetereoatoms independently selected from O, S and N; which ring is substituted on an available carbon atom by a substituent selected from R2, and is optionally further substituted on 1 or 2 available carbon atoms by a substituent independently selected from R3 and/or on an available nitrogen atom (provided it is not thereby quaternised) by a substituent selected from R10; R2 is selected from -C(O)NR4R5 and -SO2NR4R5; R3 is selected from halo;
R4 and R5 together with the nitrogen atom to which they are attached form a 4 to 7 membered saturated or partially unsaturated heterocyclyl ring, optionally containing 1 or 2 further heteroatoms (in addition to the linking N atom) independently selected from O, N and S, wherein a -CH2- group can optionally be replaced by a -C(O)- and wherein a sulphur atom in the ring may optionally be oxidised to a S(O) or S(O)2 group; which ring is optionally substituted on an available carbon atom by 1 or 2 substituents independently selected from R8 and/or on an available nitrogen atom by a substituent selected from R9; R8 is selected from hydroxy, (l-4C)alkoxy and (l-4C)alkyl; R9 is selected from (l-4C)alkyl, -C(O)(I -4C)alkyl, and -S(0)p(l-4C)alkyl;
R10 is selected from (l-4C)alkyl, -C(O)(I -4C)alkyl, benzyl, and (l-4C)alkylsulfonyl; p is (independently at each occurrence) O, 1 or 2.
In one aspect of the invention there is provided a compound of formula (I) or (IA) as hereinbefore defined, or a salt thereof, wherein: R1 is selected from isopropyl, but-2-yl, l,l,l-trifluoroprop-2-yl, l,3-difluoroprop-2-yl, but-l-yn-3-yl, l-hydroxyprop-2-yl, 2-hydroxybut-3-yl, tetrahydrofuryl, tetrahydropyranyl, l-methoxyρrop-2-yl, 2-hydroxyprop-l-yl, 2-methoxyprop-l-yl, 2-hydroxybut-l-yl, 2- methoxybut-1-yl, l-fluoromethoxyprop-2-yl and l,l-difluoromethoxyprop-2-yl; HET-I is a 5- or 6-membered, C-linked heteroaryl ring containing a nitrogen atom in the 2- position and optionally 1 or 2 further ring heteroatoms independently selected from O, N and S; which ring is optionally substituted on any nitrogen atom (provided it is not thereby quaternised) by a methyl or ethyl group and/or on 1 or 2 available carbon atoms by a methyl or ethyl group; HET-2 is a 5- or 6- membered heteroaryl ring, containing 1, 2 or 3 ring hetereoatoms independently selected from O, S and N; which ring is substituted on an available carbon atom by a substituent selected from R2, and is optionally further substituted on 1 or 2 available carbon atoms by a substituent independently selected from R3 and/or on an available nitrogen atom (provided it is not thereby quaternised) by a substituent selected from R10;
R2 is selected from -C(O)NR4R5 and -SO2NR4R5;
R3 is selected from halo; R4 and R5 together with the nitrogen atom to which they are attached form a 4 to 7 membered saturated or partially unsaturated heterocyclyl ring, optionally containing 1 or 2 further heteroatoms (in addition to the linking N atom) independently selected from O, N and S, wherein a -CH2- group can optionally be replaced by a -C(O)- and wherein a sulphur atom in the ring may optionally be oxidised to a S(O) or S(O)2 group; which ring is optionally substituted on an available carbon atom by 1 or 2 substituents independently selected from R8 and/or on an available nitrogen atom by a substituent selected from R9;
R8 is selected from hydroxy, (l-4C)alkoxy and (l-4C)alkyl;
R9 is selected from (l-4C)alkyl, -C(O)(I -4C)alkyl, and-S(0)p(l-4C)alkyl;
R10 is selected from (l-4C)alkyl, -C(O)(I -4C)alkyl, benzyl, and (l-4C)alkylsulfonyl; p is (independently at each occurrence) O, 1 or 2.
In another aspect of the invention there is provided a compound of formula (I) or
(IA) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from isopropyl, but-2-yl, cyclopentyl, l,l,l-trifluoroprop-2-yl, 1,3- difluoroprop-2-yl, but-l-yn-3-yl, l-hydroxyprop-2-yl, 2-hydroxybut-3-yl, tetrahydrofuryl, tetrahydropyranyl, l-methoxyprop-2-yl, 2-hydroxyprop-l-yl, 2-methoxyprop-l-yl, 2- hydroxybut-1-yl, 2-methoxybut-l-yl, l-fluoromethoxyprop-2-yl and 1,1- difluoromethoxyprop-2-yl;
HET-I is a 5- or 6-membered, C-linked heteroaryl ring containing a nitrogen atom in the 2- position and optionally 1 or 2 further ring heteroatoms independently selected from O, N and S; which ring is optionally substituted on any nitrogen atom (provided it is not thereby quaternised) by a methyl, ethyl or isopropyl group and/or on 1 or 2 available carbon atoms by a methyl, ethyl or fluoro group;
HET-2 is a 5- or 6- membered heteroaryl ring, containing 1, 2 or 3 ring hetereoatoms independently selected from O, S and N; which ring is substituted on an available carbon atom by a substituent selected from R2, and is optionally further substituted on 1 or 2 available carbon atoms by a substituent independently selected from R3 and/or on an available nitrogen atom (provided it is not thereby quaternised) by a substituent selected
R2 is selected from -C(O)NR4R5 and -SO2NR4R5;
R3 is selected from halo; R4 and R5 together with the nitrogen atom to which they are attached form a 4 to 7 membered saturated or partially unsaturated heterocyclyl ring, optionally containing 1 or 2 further heteroatoms (in addition to the linking N atom) independently selected from O, N and S, wherein a -CH2- group can optionally be replaced by a -C(O)- and wherein a sulphur atom in the ring may optionally be oxidised to a S(O) or S(O)2 group; which ring is optionally substituted on an available carbon atom by 1 or 2 substituents independently selected from R8 and/or on an available nitrogen atom by a substituent selected from R9;
R8 is selected from hydroxy, (l-4C)alkoxy and (l-4C)alkyl;
R9 is selected from (l-4C)alkyl, -C(O)(I -4C)alkyl, and -S(0)p(l-4C)alkyl;
R10 is selected from (l-4C)alkyl, -C(O)(I -4C)alkyl, benzyl, and (l-4C)alkylsulfonyl; p is (independently at each occurrence) O, 1 or 2.
In another aspect of the invention there is provided a compound of formula (I) or
(IA) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from isopropyl, but-2-yl, cyclopentyl, l,l,l-trifluoroprop-2-yl, 1,3- difluoroprop-2-yl, but-l-yn-3-yl, l-hydroxyprop-2-yl, 2-hydroxybut-3-yl, tetrahydrofuryl, tetrahydropyranyl, l-methoxyprop-2-yl, 2-hydroxyprop-l-yl, 2-methoxyprop-l-yl, 2- hydroxybut-1-yl, 2-methoxybut-l-yl, l-fluoromethoxyprop-2-yl and 1,1- difluoromethoxyprop-2-yl;
HET-I is a 5- or 6-membered, C-linked heteroaryl ring containing a nitrogen atom in the 2- position and optionally 1 or 2 further ring heteroatoms independently selected from O, N and S; which ring is optionally substituted on any nitrogen atom (provided it is not thereby quaternised) by a methyl, ethyl or isopropyl group and/or on 1 or 2 available carbon atoms by a methyl, ethyl or fluoro group;
HET-2 is a 5 -or 6- membered heteroaryl ring, containing 1, 2 or 3 ring hetereoatoms independently selected from O, S and N; which ring is substituted on an available carbon atom by a substituent selected from R2, and is optionally further substituted on 1 or 2 available carbon atoms by a substituent independently selected from R3 and/or on an available nitrogen atom (provided it is not thereby quaternised) by a substituent selected from R10;
R2 is selected from -C(O)NR4R5 and -SO2NR4R5;
R3 is selected from methyl and halo; R4 and R5 together with the nitrogen atom to which they are attached form a 4 to 7 membered saturated or partially unsaturated heterocyclyl ring, optionally containing 1 or 2 further heteroatoms (in addition to the linking N atom) independently selected from O, N and S, wherein a -CH2- group can optionally be replaced by a -C(O)- and wherein a sulphur atom in the ring may optionally be oxidised to a S(O) or S(O)2 group; which ring is optionally substituted on an available carbon atom by 1 or 2 substituents independently selected from R8 and/or on an available nitrogen atom by a substituent selected from R9;
R8 is selected from hydroxy, (l-4C)alkoxy and (l-4C)alkyl;
R9 is selected from (l-4C)alkyl, -C(O)(I -4C)alkyl, and-S(0)p(l-4C)alkyl;
R10 is selected from (l-4C)alkyl, -C(O)(I -4C)alkyl, benzyl, and (l-4C)alkylsulfonyl; p is (independently at each occurrence) 0, 1 or 2.
In another aspect of the invention there is provided a compound of formula (I) or
(IA) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from isopropyl, but-2-yl, cyclopentyl, l,l,l-trifluoroprop-2-yl, 1,3- difluoroprop-2-yl, but-l-yn-3-yl, l-hydroxyprop-2-yl, 2-hydroxybut-3-yl, tetrahydrofuryl, tetrahydropyranyl, l-methoxyprop-2-yl, 2-hydroxyprop-l-yl, 2-methoxyprop-l-yl, 2- hydroxybut-1-yl, 2-methoxybut-l-yl, l-fluoromethoxyprop-2-yl and 1,1- difluoromethoxyprop-2-yl;
HET-I is a 5- or 6-membered, C-linked heteroaryl ring containing a nitrogen atom in the 2- position and optionally 1 or 2 further ring heteroatoms independently selected from O, N and S; which ring is optionally substituted on any nitrogen atom (provided it is not thereby quaternised) by a methyl, ethyl or isopropyl group and/or on 1 or 2 available carbon atoms by a methyl, ethyl or fluoro group;
HET-2 is a 5- or 6- membered heteroaryl ring, containing 1, 2 or 3 ring hetereoatoms independently selected from O, S and N; which ring is substituted on an available carbon atom by a substituent selected from R2, and is optionally further substituted on 1 or 2 available carbon atoms by a substituent independently selected from R3;
R2 is selected from -C(O)NR4R5 and -SO2NR4R5; R3 is selected from methyl and halo;
R4 and R5 together with the nitrogen atom to which they are attached form a 4 to 7 membered saturated or partially unsaturated heterocyclyl ring, optionally containing 1 or 2 further heteroatoms (in addition to the linking N atom) independently selected from O, N and S, wherein a -CH2- group can optionally be replaced by a -C(O)- and wherein a sulphur atom in the ring may optionally be oxidised to a S(O) or S(O)2 group; or
R4 and R5 together with the nitrogen atom to which they are attached form a 6-10 membered bicyclic saturated or partially unsaturated heterocyclyl ring, optionally containing 1 further nitrogen atom (in addition to the linking N atom), wherein a -CH2- group can optionally be replaced by a -C(O)-.
In another aspect of the invention there is provided a compound of formula (I) or
(IA) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from isopropyl, but-2-yl, l,3-difluoroprop-2-yl, l-hydroxyprop-2-yl and 1- methoxyprop-2-yl and tetrahydrofuryl; HET-I is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
HET-2 is a substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
R2 is -CONR4R5;
R4 and R5 together form an azetidinyl, pyrrolidinyl or morpholino ring. In another aspect of the invention there is provided a compound of formula (I) or
(IA) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from isopropyl, l,3-difluoroprop-2-yl, but-2-yl, l-hydroxyprop-2-yl, 2- hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, l-hydroxybut-2-yl and 1- methoxyprop-2-yl; HET-I is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
HET-2 is a substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
R2 is -CONR4R5;
R4 and R5 together form an azetidinyl, pyrrolidinyl or morpholino ring. In another aspect of the invention there is provided a compound of formula (I) or
(IA) as hereinbefore defined, or a salt thereof, wherein: R1 is selected from isopropyl, cyclopentyl, l,3-difluoroprop-2-yl, but-2-yl, 1- hydroxyprop-2-yl, 2-hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, tetrahydropyranyl, l-hydroxybut-2-yl and l-methoxyprop-2-yl;
HET-I is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
HET-2 is a substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
R2 is -CONR4R5;
R4 and R5 together form an azetidinyl or pyrrolidinyl ring.
In another aspect of the invention there is provided a compound of formula (I) or (IA) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from isopropyl, l,3-difluoroprop-2-yl, l-hydroxyprop-2-yl and 1- methoxyprop-2-yl,
HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl and pyrazinyl, wherein R1 is optionally substituted on carbon or nitrogen with a methyl or ethyl group; HET-2 is selected from pyridyl, pyrazinyl, thiazolyl and thienyl, substituted by R2 and optionally substituted by R3;
R3 is fluoro or chloro;
R2 is -CONR4R5;
R4 and R5 together form an azetidinyl, pyrrolidinyl or morpholino ring. In another aspect of the invention there is provided a compound of formula (I) or
(IA) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from isopropyl, l,3-difluoroprop-2-yl, but-2-yl, l-hydroxyprop-2-yl, 2- hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, l-hydroxybut-2-yl and 1- methoxyprop-2-yl; HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl and pyrazinyl, wherein R1 is optionally substituted with a methyl, isopropyl or ethyl group;
HET-2 is selected from pyridyl, pyrazinyl, thiazolyl and pyrimidinyl, substituted by R2 and optionally substituted by R3;
R3 is fluoro or chloro; R2 is -CONR4R5;
R4 and R5 together form an azetidinyl, pyrrolidinyl or morpholino ring. In another aspect of the invention there is provided a compound of formula (I) or
(IA) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from isopropyl, cyclopentyl, l,3-difluoroprop-2-yl, but-2-yl, 1- hydroxyprop-2-yl, 2-hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, tetrahydropyranyl, l-hydroxybut-2-yl and l-methoxyprop-2-yl;
HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl, pyridyl, isoxazolyl and pyrazinyl, wherein R1 is optionally substituted with a methyl, isopropyl or ethyl group and/or (on a carbon atom) by fluoro;
HET-2 is selected from pyridyl, pyrazinyl, pyridazinyl, thiazolyl and pyrimidinyl, substituted by R2 and optionally substituted by R3;
R3 is fluoro or chloro;
R2 is -CONR4R5;
R4 and R5 together form an azetidinyl or pyrrolidinyl ring.
In another aspect of the invention there is provided a compound of formula (I) or (IA) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from isopropyl, cyclopentyl, l,3-difluoroprop-2-yl, but-2-yl, 1- hydroxyprop-2-yl, 2-hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, tetrahydropyranyl, l-hydroxybut-2-yl and l-methoxyprop-2-yl;
HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl, pyridyl, isoxazolyl and pyrazinyl, wherein R1 is optionally substituted with a methyl, isopropyl or ethyl group and/or (on a carbon atom) by fluoro;
HET-2 is selected from pyridyl, pyrazinyl, pyridazinyl, thiazolyl and pyrimidinyl, substituted by R2 and optionally substituted by R3;
R3 is methyl, fluoro or chloro; R2 is -CONR4R5;
R4 and R5 together form an azetidinyl, 7-azabicyclo[2.2.1]hept-7-yl, morpholino, or pyrrolidinyl ring.
In another aspect of the invention there is provided a compound of formula (I) or
(IA) as hereinbefore defined, or a salt thereof, wherein: R1 is selected from isopropyl, cyclopentyl, l,3-difluoroprop-2-yl, but-2-yl, 1- hydroxyprop-2-yl, 2-hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, tetrahydropyranyl, l-hydroxybut-2-yl and l-methoxyprop-2-yl; HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl, pyridyl, isoxazolyl and pyrazinyl, wherein R1 is optionally substituted with a methyl, isopropyl or ethyl group and/or (on a carbon atom) by fluoro;
HET-2 is selected from pyridyl, pyrazinyl, pyridazinyl, thiazolyl and pyrimidinyl, substituted by R2 and optionally substituted by R3;
R3 is methyl, fluoro or chloro;
R2 is -CONR4R5;
R4 and R5 together form an azetidinyl or pyrrolidinyl ring.
In another aspect of the invention there is provided a compound of formula (I) or (IA) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from isopropyl, cyclopentyl, l,3-difluoroprop-2-yl, but-2-yl, 1- hydroxyprop-2-yl, 2-hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, tetrahydropyranyl, l-hydroxybut-2-yl and l-methoxyprop-2-yl;
HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl, pyridyl, isoxazolyl and pyrazinyl, wherein R1 is optionally substituted on a nitrogen atom with methyl, difluoromethyl, isopropyl or ethyl and/or on a carbon atom by fluoro or methyl; provided that when HET-I is pyrazolyl, R1 is selected from l-methoxyprop-2-yl, isopropyl, and tetrahydrofuryl;
HET-2 is selected from pyridyl, pyrazinyl, pyridazinyl, thiazolyl and pyrimidinyl, substituted by R2 and optionally substituted by R3; R3 is methyl, fluoro or chloro;
R2 is -CONR4R5 or -SO2NR4R5, particularly -CONR4R5;
R4 and R5 together form an azetidinyl, 7-azabicyclo[2.2.1]hept-7-yl, morpholino, or pyrrolidinyl ring.
In another aspect, Aspect A, of the invention there is provided a compound of formula (I) or (IA) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from isopropyl, cyclopentyl, l,3-difluoroprop-2-yl, but-2-yl, 1- hydroxyprop-2-yl, 2-hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, tetrahydropyranyl, l-hydroxybut-2-yl and l-methoxyprop-2-yl;
HET-I is selected from pyrazinyl (optionally substituted with methyl), pyrazolyl (optionally substituted on carbon by methyl), methylthiadiazolyl (particularly 3-methyl- l,2,4-thiadiazol-5-yl), thiazolyl (optionally substituted with methyl), pyridyl (optionally substituted by fluoro) and isoxazolyl; provided that when HET-I is pyrazolyl, particularly
R1 is selected from l-methoxyprop-2-yl, isopropyl, and tetrahydrofuryl;
HET-2 is selected from pyridyl, pyrazinyl, pyridazinyl, thiazolyl and pyrimidinyl, substituted by R2 and optionally substituted by R3; R3 is methyl, fluoro or chloro;
R2 is -CONR4R5 Or-SO2NR4R5, particularly -CONR4R5;
R4 and R5 together form an azetidinyl, 7-azabicyclo[2.2.1]hept-7-yl, morpholino, or pyrrolidinyl ring.
In another aspect, Aspect B, of the invention there is provided a compound of formula (I) or (IA) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from isopropyl, cyclopentyl, l,3-difluoroprop-2-yl, but-2-yl, 1- hydroxyprop-2-yl, 2-hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, tetrahydropyranyl, l-hydroxybut-2-yl and l-methoxyprop-2-yl;
HET-I is selected from pyrazinyl (optionally substituted with methyl), pyrazolyl (optionally substituted on carbon by methyl), methylthiadiazolyl (particularly 3-methyl- l,2,4-thiadiazol-5-yl), thiazolyl (optionally substituted with methyl), pyridyl (optionally substituted by fluoro) and isoxazolyl; provided that when HET-I is pyrazolyl, particularly
R1 is selected from l-methoxyprop-2-yl, isopropyl, and tetrahydrofuryl;
HET-2 is selected from pyridyl and pyrazinyl, substituted by R2 and optionally substituted by R3;
R3 is methyl, fluoro or chloro;
R2 is -CONR4R5 or -SO2NR4R5, particularly -CONR4R5;
R4 and R5 together form an azetidinyl or pyrrolidinyl ring, particularly azetidinyl.
Particular compounds of Aspect B are those of formulae (IB), (IC) and/or (ID). In another aspect, Aspect C, of the invention there is provided a compound of formula (IB), (IC) or (ID) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from isopropyl, l-hydroxyprop-2-yl, tetrahydrofuryl and l-methoxyprop-2- yl; (particularly R1 is selected from isopropyl, (2S)- l-hydroxyprop-2-yl, tetrahydrofuryl and (2S)- l-methoxyprop-2-yl); HET-I is methylpyrazinyl;
R2 is -CONR4R5;
R4 and R5 together form an azetidinyl ring. In another aspect, Aspect D, of the invention there is provided a compound of formula (IB) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from isopropyl, l-hydroxyprop-2-yl, tetrahydrofuryl and l-methoxyprop-2- yl; (particularly R1 is selected from isopropyl, (2S)- l-hydroxyprop-2-yl, tetrahydrofuryl and (2S)-l-methoxyprop-2-yl);
HET-I is methylpyrazinyl;
R2 is -CONR4R5;
R4 and R5 together form an azetidinyl ring.
In another aspect, Aspect E, of the invention there is provided a compound of formula (IB), (IC) or (ID) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from isopropyl, tetrahydrofuryl and l-methoxyprop-2-yl (particularly (2S)-
1 -methoxyprop-2-yl) ;
HET-I is pyrazolyl;
R2 is -CONR4R5; R4 and R5 together form an azetidinyl ring.
In another aspect, Aspect F, of the invention there is provided a compound of formula (IB) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from isopropyl, tetrahydrofuryl and l-methoxyprop-2-yl (particularly (2S)-
1 -methoxyprop-2-yl); HET-I is pyrazolyl;
R2 is -CONR4R5;
R4 and R5 together form an azetidinyl ring.
In another aspect of the invention there is provided a compound of formula (I) (or
IA) as hereinbefore defined, or a salt thereof, wherein: R1 is selected from isopropyl, but-2-yl, l,3-difluoroprop-2-yl, l-hydroxyprop-2-yl and 1- methoxyprop-2-yl and tetrahydrofuryl;
HET-I is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
HET-2 is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
R2 is -SO2NR4R5;
R4 and R5 together form an azetidinyl, pyrrolidinyl or morpholino ring. In another aspect of the invention there is provided a compound of formula (I) (or
IA) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from isopropyl, l,3-difluoroprop-2-yl, l-hydroxyprop-2-yl and 1- methoxyprop-2-yl, HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl and pyrazinyl, wherein R1 is optionally substituted on carbon or nitrogen with a methyl or ethyl group;
HET-2 is selected from pyridyl, pyrazinyl, thiazolyl and thienyl, substituted by R2 and optionally substituted by R3;
R3 is fluoro or chloro; R2 is -SO2NR4R5;
R4 and R5 together form an azetidinyl, pyrrolidinyl or morpholino ring.
In another aspect of the invention there is provided a compound of formula (I) (or
IA) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from isopropyl, l,3-difluoroprop-2-yl, but-2-yl, l-hydroxyprop-2-yl, 2- hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, l-hydroxybut-2-yl and 1- methoxyprop-2-yl;
HET-I is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
HET-2 is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
R2 Is -SO2NR4R5;
R4 and R5 together form an azetidinyl, pyrrolidinyl or morpholino ring.
In another aspect of the invention there is provided a compound of formula (I) (or
IA) as hereinbefore defined, or a salt thereof, wherein: R1 is selected from isopropyl, 1, 3 -difluoroprop-2-yl, but-2-yl, l-hydroxyprop-2-yl, 2- hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, l-hydroxybut-2-yl and 1- methoxyprop-2-yl;
HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl and pyrazinyl, wherein R1 is optionally substituted with a methyl, isopropyl or ethyl group; HET-2 is selected from pyridyl, pyrazinyl, thiazolyl and pyrimidinyl, substituted by R2 and optionally substituted by R3;
R3 is fluoro or chloro; R2 Is -SO2NR4R5;
R4 and R5 together form an azetidinyl, pyrrolidinyl or morpholino ring.
In another aspect of the invention there is provided a compound of formula (I) (or
IA) as hereinbefore defined, or a salt thereof, wherein: R1 is selected from isopropyl, l,3-difluoroprop-2-yl, but-2-yl, l-hydroxyprop-2-yl, 2- hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, l-hydroxybut-2-yl and 1- methoxyprop-2-yl;
HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl and pyrazinyl, wherein R1 is optionally substituted with a methyl, isopropyl or ethyl group; HET-2 is selected from pyridyl, pyrazinyl, thiazolyl and pyrimidinyl, substituted by R2 and optionally substituted by R3;
R3 is fluoro or chloro;
R2 is -CONR4R5 Or-SO2NR4R5;
R4 and R5 together form an azetidinyl, pyrrolidinyl or morpholino ring. In one aspect of the invention there is provided a compound of formula (I) (or IA) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from l-fluoromethoxyprop-2-yl and 1, l-difluoromethoxyprop-2-yl;
HET-I is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined; HET-2 is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
R2 is -CONR4R5;
R4 and R5 together form an azetidinyl, pyrrolidinyl or morpholino ring.
In another aspect of the invention there is provided a compound of formula (I) (or IA) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from l-fluoromethoxyprop-2-yl and 1, l-difluoromethoxyprop-2-yl;
HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl and pyrazinyl, wherein R1 is optionally substituted on carbon or nitrogen with a methyl or ethyl group;
HET-2 is selected from pyridyl, pyrazinyl, thiazolyl and thienyl, substituted by R2 and optionally substituted by R3;
R3 is fluoro or chloro;
R2 is -CONR4R5; R4 and R5 together form an azetidinyl, pyrrolidinyl or morpholino ring.
In one aspect of the invention there is provided a compound of formula (I) (or IA) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from l-fiuoromethoxyρrop-2-yl and l,l-difluoromethoxyprop-2-yl; HET-I is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
HET-2 is an optionally substituted 5- or 6-membered heteroaryl ring as hereinbefore defined;
R2 is -SO2NR4R5; R4 and R5 together form an azetidinyl, pyrrolidinyl or morpholino ring.
In another aspect of the invention there is provided a compound of formula (I) (or
IA) as hereinbefore defined, or a salt thereof, wherein:
R1 is selected from l-fluoromethoxyprop-2-yl and l,l-difluoromethoxyprop-2-yl;
HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl and pyrazinyl, wherein R1 is optionally substituted on carbon or nitrogen with a methyl or ethyl group;
HET-2 is selected from pyridyl, pyrazinyl, thiazolyl and thienyl, substituted by R2 and optionally substituted by R3;
R3 is fluoro or chloro;
R2 is -SO2NR4R5; R4 and R5 together form an azetidinyl, pyrrolidinyl or morpholino ring.
Further preferred compounds of the invention are each of the Examples (and salts thereof), each of which provides a further independent aspect of the invention. In further aspects, the present invention also comprises any two or more compounds of the Examples (and salts thereof).
Particular compounds of the invention include any one or more of:
3-{[5-(azetidin-l-ylcarbonyl)pyridin-2-yl]oxy}-5-{[(lS)-2-hydroxy-l-methylethyl]oxy}-
N-l,3-thiazol-2-ylbenzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(lS)-2-hydroxy-l- methylethyl]oxy}-N- 1 ,3-thiazol-2-ylbenzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-{[(lS)-2-hydroxy-l-methylethyl]oxy}-
N-l,3-thiazol-2-ylbenzamide; 3-{[5-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-{[(lS)-2-hydroxy-l-methylethyl]oxy}-
N-(I -methyl- 1 H-pyrazol-3 -yl)benzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-{[(lS)-2-hydroxy-l-methylethyl]oxy}-
N-(I -methyl- 1 H-pyrazol-3 -yl)benzamide; 3-{[5-(azetidin-l-ylcarbonyl)pyridin-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy } -N-( 1 -methyl- 1 H-pyrazol-3-yl)benzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl]oxy } -5 -{[(1S)-1 -methyl-2-
(methyloxy)ethyl] oxy } -N-( 1 -methyl- 1 H-pyrazol-3 -yl)benzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)pyridin-2-yl]oxy } -5 - [( 1 -methylethyl)oxy] -N- 1 ,3 -thiazol-2- ylbenzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl]oxy } -5- [( 1 -methylethyl)oxy] -N- 1,3- thiazol-2-ylbenzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyridin-2-yl]oxy}-5-[(l-methylethyl)oxy]-N-(l-methyl-lH- pyrazol-3-yl)benzamide; 3 - { [5 -(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl] oxy } -5-[( 1 -methylethyl)oxy] -N-( 1 - methyl-lH-pyrazol-3-yl)benzamide;
3- { [5-(azetidin- 1 -ylcarbonyl)pyridin-2-yl]oxy }-5- { [( 1 S)- 1 -methyl-2-
(methyloxy)ethyl]oxy}-N-(3-methyl-l,2,4-thiadiazol-5-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(lS)-l-methyl-2- (methyloxy)ethyl]oxy}-N-(3-methyl-l,2,4-thiadiazol-5-yl)benzamide;
3- { [6-(azetidin- 1 -ylcarbonyl)pyridin-3-yl]oxy } -5- { [(I S)- 1 -methyl-2-
(methyloxy)ethyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide; 3-{[5-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-[(l-methylethyl)oxy]-N-(l-methyl-lH- pyrazol-3-yl)benzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-[(l-methylethyl)oxy]-N-l,3-thiazol-2- ylbenzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-[(l-methylethyl)oxy]-N-(l-methyl-lH- pyrazol-3-yl)benzamide;
3-{ [6-(azetidin- 1 -ylcarbonyl)pyridin-3-yl]oxy}-5-{ [(I S)- 1 -methyl-2-
(methyloxy)ethyl]oxy}-N-l,3-thiazol-2-ylbenzamide; 3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-(3-methyl-l,2,4-thiadiazol-5-yl)benzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-[(l-methylethyl)oxy]-N-(3-methyl-l,2,4- thiadiazol-5-yl)benzamide; 3-{[5-(azetidin-l-ylsulfonyl)ρyridin-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy } -N-( 1 -methyl- 1 H-pyrazol-3 -yl)benzamide ;
3-{[5-(azetidin-l-ylcarbonyl)-l,3-thiazol-2-yl]oxy}-5-[(l-methylethyl)oxy]-N-(l-methyl- lH-pyrazol-3-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyridin-2-yl]oxy}-5-{[(lS)-2-hydroxy-l-methylethyl]oxy}-
N-(I -methyl- 1 H-pyrazol-3 -yl)benzamide;
3-{[3-chloro-5-(morpholin-4-ylcarbonyl)pyridin-2-yl]oxy}-5-{[(lS)-2-hydroxy-l- methylethyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide; and 3- { [5-(azetidin- 1 -ylcarbonyl)-3-chloropyridin-2-yl]oxy } -5- { [2-fluoro- 1 -
(fluoromethyl)ethyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide; and/or
3-{[5-(azetidin-l-ylcarbonyl)-l,3-thiazol-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(lS)-l-methylpropyl]oxy}-N- (1 -methyl- 1 H-pyrazol-3 -yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{[(lS)-l-methylpropyl]oxy}-N-(l- methyl- 1 H-pyrazol-3 -yl)benzamide;
3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3 -yl]oxy } -5 - { [( IS)-I -methylpropyl] oxy } -N-( 1 - methyl- 1 H-pyrazol-3 -yl)benzamide; 3-{[2-(azetidin-l-ylcarbonyl)pyrimidin-5-yl]oxy}-5-[(l-methylethyl)oxy]-N-(l-methyl-
1 H-pyrazol-3 -yl)benzamide;
3-{[2-(azetidin-l-ylcarbonyl)pyrirnidin-5-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-{[2-fluoro-l-(fluoromethyl)ethyl]oxy}- N-(l-methyl-lH-pyrazol-3-yl)benzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)pyrazin-2-yl] oxy } -5- { [2-fluoro- 1 -(fluoromethyl)ethyl] oxy } -
N-(I -methyl- 1 H-pyrazol-3 -yl)benzamide; 3-{ [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy}-5-[(l -methylethyl)oxy]-N-(l -methyl- IH- pyrazol-3-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-({(lS)-2-
[(difluoromethyl)oxy] - 1 -methylethyl } oxy)-N-( 1 -methyl- 1 H-pyrazol-3 -yl)benzamide; 3 - { [5-(azetidin- 1 -ylcarbonyl)pyridin-2-yl]oxy } -5-({ ( 1 S)-2-[(difluoromethyl)oxy]- 1 - methylethyl } oxy)-N-( 1 -methyl- 1 H-pyrazol-3 -yl)benzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-({(lS)-2-[(difluoromethyl)oxy]-l- methylethyl } oxy)-N-( 1 -methyl- 1 H-pyrazol-3 -yl)benzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)pyrazin-2-yl] oxy } -5-( { ( 1 S)-2- [(difluoromethyl)oxy]- 1 - methylethyl } oxy)-N-( 1 -methyl- 1 H-pyrazol-3 -yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(lR,2R)-2-hydroxy-l- methylpropyl] oxy } -N-(I -methyl- 1 H-pyrazol-3 -yl)benzamide;
3- { [5-(azetidin- 1 -ylcarbonyl)-3-chloropyridin-2-yl]oxy } -5- { [( 1 S,2S)-2-hydroxy- 1 - methylpropyl] oxy } -N-( 1 -methyl- 1 H- pyrazol-3 -yl)benzamide; 3 - { [5 -(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl] oxy } -N-( 1 -ethyl- 1 H-pyrazol-3 -yl)-5-
{ [( 1 S)- 1 -methyl-2-(methyloxy)ethyl]oxy }benzamide;
3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3 -yljoxy } -N-( 1 -ethyl- 1 H-pyrazol-3 -yl)-5- { [(1S)-I- methyl-2-(methyloxy)ethyl] oxy } benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-N-(l-ethyl-lH-pyrazol-3-yl)-5-{[(lS)-l- methyl-2-(methyloxy)ethyl] oxy } benzamide;
3- { [5-(azetidin- 1 -ylcarbonyl)-3-chloropyridin-2-yl]oxy } -5- { [( 1 S)-2-hydroxy- 1 - methylethyl] oxy } -N-[ 1 -( 1 -methylethyl)- 1 H-pyrazol-3-yl]benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyridin-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-lH-pyrazol-3-ylbenzamide; 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)-5-
[(3 S)-tetrahydrofuran-3 -yloxy]benzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)-5-[(3S)- tetrahydrofuran-3-yloxy]benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)-5-[(3S)- tetrahydrofuran-3-yloxy]benzamide;
3 - { [4-(azetidin- 1 -ylcarbonyl)- 1 ,3-thiazol-2-yl]oxy }-5- { [(I S)- 1 -methyl-2-
(methyloxy)ethyl] oxy } -N-( 1 -methyl- 1 H-pyrazol-3 -yl)benzamide; 3-{[4-(azetidin-l-ylcarbonyl)-l,3-thiazol-2-yl]oxy}-5-[(l-methylethyl)oxy]-N-(l-methyl- lH-pyrazol-3-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{[(lS)-l-(hydroxymethyl)propyl]oxy}-
N-( 1 -methyl- 1 H-pyrazol-3-yl)benzamide; and/or 3-{ [2-(azetidin- 1 -ylcarbonyl)pyrimidin-5-yl]oxy}-5-{ [(I S)- 1 -methyl-2-
(methyloxy)ethyl]oxy}-N-l,3-thiazol-2-ylbenzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy } -5 -(cyclopentyloxy)-N-( 1 -methyl- 1 H- pyrazol-3-yl)benzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)-3-chloropyridin-2-yl] oxy } -5 -(cyclopentyloxy)-N-( 1 -methyl- lH-pyrazol-3-yl)benzamide;
3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3 -yl] oxy } -5 -(cyclopentyloxy)-N-( 1 -methyl- 1 H- pyrazol-3 -yl)benzamide ;
3 - { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl] oxy } -5 - { [( 1 R,2R)-2-hydroxy- 1 - methylpropyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide; 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{[(lS,2S)-2-hydroxy-l- methylpropyl]oxy } -N-(I -methyl- 1 H-pyrazol-3-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-l,3-thiazol-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(lS)-l-methyl-2- (methyloxy)ethyl]oxy } -N-(5-methylpyrazin-2-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyridin-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide; 3-{[4-(azetidin-l-ylcarbonyl)-l,3-thiazol-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-4-methyl-l33-thiazol-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-4-methyl-l,3-thiazol-2-yl]oxy}-5-{[(lS)-l-methyl-2- (methyloxy)ethyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-[(l-methylethyl)oxy]-N-(5- methylpyrazin-2-yl)benzamide; 3- { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy } -5-[( 1 -methylethyl)oxy]-N-(5- methylpyrazin-2-yl)benzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)pyridin-2-yl] oxy } -5 - [( 1 -methylethyl)oxy] -N-(5- methylpyrazin-2-yl)benzamide; 3-{[5-(azetidin-l-ylcarbonyl)-4-methyl-l,3-thiazol-2-yl]oxy}-5-[(l-methylethyl)oxy]-N-
(5-methylpyrazin-2-yl)benzamide;
3- { [6-(azetidin- 1 -ylcarbonyl)pyridin-3-yl]oxy }-5- { [( 1 S)- 1 -methyl-2-
(methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide;
3-{[6-(azetidm-l-ylcarbonyl)pyridin-3-yl]oxy}-5-[(l-methylethyl)oxy]-N-(5- methylpyrazin-2-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-lH-pyrazol-3-ylbenzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-l,3-thiazol-2-ylbenzamide; 3- { [5-(azetidin- 1 -ylcarbonyl)-3-chloropyridin-2-yl]oxy }-5- { [( 1 S)- 1 -methyl-2-
(methyloxy)ethyl]oxy}-N-pyridin-2-ylbenzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl] oxy } -N-( 1 -ethyl- 1 H-pyrazol-3 -yl)-5-
[(I -methylethyl)oxy]benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-N-(l-ethyl-lH-pyrazol-3-yl)-5-[(l- methylethyl)oxy]benzamide;
3- { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy } -N- [ 1 -( 1 -methylethyl)- 1 H-pyrazol-3 -yl] -5 -
{[(lS)-l-methyl-2-(methyloxy)ethyl]oxy}benzamide;
3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3 -yl]oxy } -N-( 1 -ethyl- 1 H-pyrazol-3 -yl)-5- [( 1 - methylethyl)oxy]benzamide; 3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-pyridin-2-ylbenzamide;
3- { [6-(azetidin- 1 -ylcarbonyl)pyridin-3-yl]oxy} -N-isoxazol-3-yl-5- { [(I S)- 1 -methyl-2-
(methyloxy)ethyl]oxy}benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-[(l-methylethyl)oxy]-N-(3- methyl- 1 ,2,4-thiadiazol-5-yl)benzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-[(l-methylethyl)oxy]-N-lH-pyrazol-3- ylbenzamide; 3- { [6-(azetidin- 1 -ylcarbonyl)pyridin-3-yl]oxy } -5- { [( 1 S)- 1 -methyl-2-
(methyloxy)ethyl]oxy}-N-lH-pyrazol-3-ylbenzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-[(l-methylethyl)oxy]-N-lH-pyrazol-3- ylbenzamide; 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-lH-pyrazol-3-ylbenzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-[(l-methylethyl)oxy]-N-lH- pyrazol-3-ylbenzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl] oxy } -N-( 1 , 5-dimethyl- 1 H-pyrazol-3 - yl)-5-{ [(I S)-l-methyl-2-(methyloxy)ethyl]oxy}benzamide;
3- { [5-(azetidin- 1 -ylcarbonyl)-3-chloropyridin-2-yl]oxy } -N-( 1 ,5-dimethyl- 1 H-pyrazol-3- yl)-5-{[2-fluoro-l-(fluoromethyl)ethyl]oxy}benzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridazin-3-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy } -N-( 1 -methyl- 1 H-pyrazol-3 -yl)benzamide ; 3-{ [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy}-N-(4-methyl- 1 ,3-thiazol-2-yl)-5-[(3 S)- tetrahydrofuran-3-yloxy]benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-N-(5-methyl-l,3-thiazol-2-yl)-5-[(3S)- tetrahydrofuran-3-yloxy]benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-N-(l-ethyl-lH-pyrazol-3-yl)-5-[(3S)- tetrahydrofuran-3-yloxy]benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-N-[l-(l-methylethyl)-lH-pyrazol-3-yl]-5-
[(3S)-tetrahydrofuran-3-yloxy]benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-N-lH-pyrazol-3-yl-5-[(3S)- tetrahydrofuran-3-yloxy]benzamide; 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-N-(l,5-dimethyl-lH-pyrazol-3-yl)-5-[(3S)- tetrahydrofuran-3-yloxy]benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-N-(4-methyl-l,3-thiazol-2-yl)-5-
[(3 S)-tetrahydrofuran-3 -yloxy]benzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl] oxy } -N-(5-methyl- 1 ,3 -thiazol-2-yl)-5- [(3 S)-tetrahydrofuran-3 -yloxy]benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-N-(5-methylpyrazin-2-yl)-5-
[(3S)-tetrahydrofuran-3-yloxy]benzamide; 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-N-(l-ethyl-lH-pyrazol-3-yl)-5-
[(3S)-tetrahydrofuran-3-yloxy]benzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)-3-chloropyridin-2-yl] oxy } -N- [ 1 -( 1 -methylethyl)- 1 H- pyrazol-3-yl]-5-[(3S)-tetrahydrofuran-3-yloxy]benzamide; 3-{[5-(azetidin-l-ylcarbonyl)-3-chloroρyridin-2-yl]oxy}-N-lH-pyrazol-3-yl-5-[(3S)- tetrahydrofuran-3-yloxy]benzamide;
3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3 -yl] oxy } -N-(5-methylpyrazin-2-yl)-5 - [(3 S)- tetrahydrofuran-3-yloxy]benzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy }-N-(5-methylpyrazin-2-yl)-5-[(3 S)- tetrahydrofuran-3 -yloxy]benzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-N-(5-fluoropyridin-2-yl)-5-[(3S)- tetrahydrofuran-3 -yloxyjbenzamide;
3-{[3-chloro-5-(pyrrolidin-l-ylcarbonyl)pyridin-2-yl]oxy}-5-[(l-methylethyl)oxy]-N-(5- methylpyrazin-2-yl)benzamide; 3-{ [S-chloro-S-føyrrolidin- 1 -ylcarbonyl)pyridin-2-yl]oxy}-5-{ [(I S)- 1 -methyl-2-
(methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide;
3 - { [3 -chloro-5 -(pyrrolidin- 1 -ylcarbonyl)pyridin-2-yl]oxy } -N-( 1 -methyl- 1 H-pyrazol-3 -yl)-
5 - [(3 S)-tetrahydrofuran-3 -yloxy]benzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl] oxy } -N-( 1 -methyl- 1 H-pyrazol-3 -yl)-5- (tetrahydro-2H-pyran-4-yloxy)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)-5-
(tetrahydro-2H-pyran-4-yloxy)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-N-(5-methylpyrazin-2-yl)-5-(tetrahydro-
2H-pyran-4-yloxy)benzamide; 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-N-(5-methylpyrazin-2-yl)-5-
(tetrahydro-2H-pyran-4-yloxy)benzamide;
3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3 -yl]oxy } -N-( 1 -methyl- 1 H-pyrazol-3 -yl)-5-
(tetrahydro-2H-pyran-4-yloxy)benzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-N-(5-methylpyrazin-2-yl)-5-(tetrahydro- 2H-pyran-4-yloxy)benzamide; and
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)-5-[(3R)- tetrahydrofuran-3-yloxy]benzamide; and/or 3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-N-isoxazol-3-yl-5-[(3S)-tetrahydrofuran-3- yloxyjbenzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl] oxy } -N-pyrazin-2-yl-5 - [(3 S)-tetrahydrofuran-3 - yloxy]benzamide ; 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-N-(5-fluoropyridin-2-yl)-5-[(3S)- tetrahydrofuran-3 -yloxy]benzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl] oxy } -5- { [( 1 S)- 1 -methyl-2-
(methyloxy)ethyl]oxy}-N-pyridin-2-ylbenzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl] oxy } -N-isoxazol-3 -yl-5- { [( 1 S)- 1 -methyl-2- (methyloxy)ethyl]oxy}benzamide;
3-{ [5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{ [(I S)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-(4-methyl-l,3-thiazol-2-yl)benzamide;
3- { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy } -5- { [( 1 S)- 1 -methyl-2-
(methyloxy)ethyl]oxy}-N-(5-methyl-l,3-thiazol-2-yl)benzamide; 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-pyrazin-2-ylbenzamide;
3-{[5-(azetidm-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-[(l-methylethyl)oxy]-N-pyrazin-2- ylbenzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-{[(lS)-l-methyl-2- (methyloxy)ethyl]oxy}-N-pyrazin-2-ylbenzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-[(l-methylethyl)oxy]-N- pyrazin-2-ylbenzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-pyrazin-2-ylbenzamide; 3-{[3-chloro-5-(moφholin-4-ylcarbonyl)pyridin-2-yl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)-
5-[(3S)-tetrahydroftιran-3-yloxy]benzamide;
3-{[5-(7-azabicyclo[2.2.1]hept-7-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-N-(l-methyl-lH- pyrazol-3-yl)-5-[(3 S)-tetrahydrofuran-3-yloxy]benzamide; and
3-{[5-(azetidin-l-ylcarbonyl)-4-methyl-l,3-thiazol-2-yl]oxy}-N-(l-methyl-lH-pyrazol-3- yl)-5-[(3S)-tetrahydrofuran-3-yloxy]benzamide; and/or
3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3-yl]oxy } -5- { [( 1 S)-2-hydroxy- 1 -methylethyl]oxy } -
N-(5 -methylpyrazin-2-yl)benzamide; 3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3 -yl] oxy } -5 - { [( 1 S)-2-hydroxy- 1 -methylethyl] oxy } -
N-(3-methyl- 1 ,2,4-thiadiazol-5-yl)benzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl] oxy } -5- { [( 1 S)-2-hydroxy- 1 - methylethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide; 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(lS)-2-hydroxy-l- methylethyl]oxy}-N-(3-methyl- 1 ,2,4-thiadiazol-5-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(lS)-2-hydroxy-l- methylethyl]oxy}-N-pyrazin-2-ylbenzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(lS)-2-hydroxy-l- methylethyl]oxy } -N-pyridin-2-ylbenzamide;
3- { [5-(azetidin- 1 -ylcarbonyl)pyridin-2-yl]oxy}-5- { [( 1 S)-2-hydroxy- 1 -methylethyljoxy } -
N-(3-methyl- 1 ,2,4-thiadiazol-5-yl)benzamide;
N-(I -methyl- 1 H-pyrazol-3-yl)-3 - { [6-(pyrrolidin- 1 -ylcarbonyl)pyridin-3-yl]oxy } -5- [(3 S)- tetrahydrofuran-3 -yloxy]benzamide; 3-{ [6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-N-pyrazin-2-yl-5-[(3S)-tetrahydrofuran-3- yloxyjbenzamide;
3- { [6-(azetidin- 1 -ylcarbonyl)pyridin-3-yl]oxy } -N-(5-methyl- 1 H-pyrazol-3-yl)-5-[(3 S)- tetrahydrofuran-3-yloxy]benzamide;
3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3-yl] oxy } -N- 1 H-pyrazol-3 -yl-5- [(3 S)- tetrahydrofuran-3 -yloxy]benzamide;
3-{ [5-(azetidin- 1 -ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{ [2-fluoro- 1 -
(fluoromethyl)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide;
3-{ [5-(azetidin- 1 -ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{ [2-fluoro- 1 -
(fluoromethyl)ethyl]oxy}-N-lH-pyrazol-3-ylbenzamide; 3- { [5-(azetidin- 1 -ylcarbonyl)-3-chloropyridin-2-yl]oxy } -5- { [2-fluoro- 1 -
(fluoromethyl)ethyl]oxy}-N-(5-methyl-lH-pyrazol-3-yl)benzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy } -5-{ [2-fluoro- 1 -(fluoromethyl)ethyl]oxy } -
N- 1 H-pyrazol-3 -ylbenzamide;
3- { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy } -5- { [2-fluoro- 1 -(fluoromethyl)ethyl]oxy } - N-(5-methylpyrazin-2-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{[(lS)-2-hydroxy-l-methylethyl]oxy}-
N-(5-methylpyrazin-2-yl)benzamide; 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-({(lS)-2-[(difluoromethyl)oxy]-l- methylethyl } oxy)-N- 1 H-pyrazol-3 -ylbenzamide;
3-{[5-(azetidin-l-ylsulfonyl)-4-methyl-l,3-thiazol-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-(5-methylρyrazin-2-yl)benzamide; 3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3 -yl]oxy } -N- [ 1 -(difluoromethyl)- 1 H-pyrazol-3 -yl]-5 -
[(3S)-tetrahydrofuran-3-yloxy]benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-N-[l-(difluoromethyl)-lH-pyrazol-3-yl]-5-
{ [( 1 S)- 1 -methyl-2-(methyloxy)ethyl]oxy }benzamide;
3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3 -yl] oxy } -N- [ 1 -(difluoromethyl)- 1 H-pyrazol-3 -yl] -5- {[(lS)-l-methyl-2-(methyloxy)ethyl]oxy}benzamide; and/or
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{[(li?)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-{[(li?)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide; or a salt thereof.
A further feature of the invention is a pharmaceutical composition comprising a compound of Formula (I), (IA), (IB), (IC) or (ID) as defined above, or a pharmaceutically- acceptable salt thereof, together with a pharmaceutically-acceptable diluent or carrier. According to another aspect of the invention there is provided the a compound of
Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically-acceptable salt thereof as defined above for use as a medicament.
According to another aspect of the invention there is provided the a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically-acceptable salt thereof as defined above for use as a medicament for treatment of a disease mediated through GLK, in particular type 2 diabetes.
Further according to the invention there is provided the use a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically-acceptable salt thereof in the preparation of a medicament for treatment of a disease mediated through GLK, in particular type 2 diabetes.
The compound is suitably formulated as a pharmaceutical composition for use in this way. According to another aspect of the present invention there is provided a method of treating GLK mediated diseases, especially diabetes, by administering an effective amount of a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically-acceptable salt thereof, to a mammal in need of such treatment. Specific diseases which may be treated by a compound or composition of the invention include: blood glucose lowering in Type 2 Diabetes Mellitus without a serious risk of hypoglycaemia (and potential to treat type 1), dyslipidemia, obesity, insulin resistance, metabolic syndrome X, impaired glucose tolerance.
As discussed above, thus the GLK/GLKRP system can be described as a potential "Diabesity" target (of benefit in both Diabetes and Obesity). Thus, according to another aspect of the invention there is provided the use of a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically-acceptable salt thereof, in the preparation of a medicament for use in the combined treatment or prevention, particularly treatment of diabetes and obesity. According to another aspect of the invention there is provided the use of a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically-acceptable salt thereof, in the preparation of a medicament for use in the treatment or prevention, particularly treatment of obesity.
According to another aspect of the invention there is provided the a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically-acceptable salt thereof as defined above for use as a medicament for treatment or prevention, particularly treatment of obesity.
According to a further aspect of the invention there is provided a method for the combined treatment of obesity and diabetes by administering an effective amount of a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically-acceptable salt thereof, to a mammal in need of such treatment.
According to a further aspect of the invention there is provided a method for the treatment of obesity by administering an effective amount of a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically-acceptable salt thereof, to a mammal in need of such treatment. Compounds of the invention may be particularly suitable for use as pharmaceuticals because of advantageous physical and/or pharmacokinetic properties, and/or favourable toxicity profile and/or favourable metabolic profile.
Favourable toxicity profile may be demonstrated, for example, by use of an Ames test assay, and/or by testing against the hERG ion channel. A favourable metabolic profile may mean, for example, reduced rate of metabolism, leading to reduction in clearance of the compound from the body and hence increased exposure to the compound, or a favourable metabolic profile may mean, for example, not forming active metabolites (which might be considered undesirable in some circumstances). For example, compounds of Aspects A to F may have favourable toxicological profiles.
The compositions of the invention may be in a form suitable for oral use (for example as tablets, lozenges, hard or soft capsules, aqueous or oily suspensions, emulsions, dispersible powders or granules, syrups or elixirs), for topical use (for example as creams, ointments, gels, or aqueous or oily solutions or suspensions), for administration by inhalation (for example as a finely divided powder or a liquid aerosol), for administration by insufflation (for example as a finely divided powder) or for parenteral administration (for example as a sterile aqueous or oily solution for intravenous, subcutaneous, intramuscular or intramuscular dosing or as a suppository for rectal dosing). Dosage forms suitable for oral use are preferred.
The compositions of the invention may be obtained by conventional procedures using conventional pharmaceutical excipients, well known in the art. Thus, compositions intended for oral use may contain, for example, one or more colouring, sweetening, flavouring and/or preservative agents. Suitable pharmaceutically acceptable excipients for a tablet formulation include, for example, inert diluents such as lactose, sodium carbonate, calcium phosphate or calcium carbonate, granulating and disintegrating agents such as corn starch or algenic acid; binding agents such as starch; lubricating agents such as magnesium stearate, stearic acid or talc; preservative agents such as ethyl or propyl p.-hydroxybenzoate, and anti-oxidants, such as ascorbic acid. Tablet formulations may be uncoated or coated either to modify their disintegration and the subsequent absorption of the active ingredient within the gastrointestinal tract, or to improve their stability and/or appearance, in either case, using conventional coating agents and procedures well known in the art.
Compositions for oral use may be in the form of hard gelatin capsules in which the active ingredient is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin, or as soft gelatin capsules in which the active ingredient is mixed with water or an oil such as peanut oil, liquid paraffin, or olive oil.
Aqueous suspensions generally contain the active ingredient in finely powdered form together with one or more suspending agents, such as sodium carboxymethylcellulose, methylcellulose, hydroxypropylmethylcellulose, sodium alginate, polyvinyl-pyrrolidone, gum tragacanth and gum acacia; dispersing or wetting agents such as lecithin or condensation products of an alkylene oxide with fatty acids (for example polyoxethylene stearate), or condensation products of ethylene oxide with long chain aliphatic alcohols, for example heptadecaethyleneoxycetanol, or condensation products of ethylene oxide with partial esters derived from fatty acids and a hexitol such as polyoxyethylene sorbitol monooleate, or condensation products of ethylene oxide with long chain aliphatic alcohols, for example heptadecaethyleneoxycetanol, or condensation products of ethylene oxide with partial esters derived from fatty acids and a hexitol such as polyoxyethylene sorbitol monooleate, or condensation products of ethylene oxide with partial esters derived from fatty acids and hexitol anhydrides, for example polyethylene sorbitan monooleate. The aqueous suspensions may also contain one or more preservatives (such as ethyl or propyl r)-hydroxybenzoate, anti-oxidants (such as ascorbic acid), colouring agents, flavouring agents, and/or sweetening agents (such as sucrose, saccharine or aspartame).
Oily suspensions may be formulated by suspending the active ingredient in a vegetable oil (such as arachis oil, olive oil, sesame oil or coconut oil) or in a mineral oil (such as liquid paraffin). The oily suspensions may also contain a thickening agent such as beeswax, hard paraffin or cetyl alcohol. Sweetening agents such as those set out above, and flavouring agents may be added to provide a palatable oral preparation. These compositions may be preserved by the addition of an anti-oxidant such as ascorbic acid. Dispersible powders and granules suitable for preparation of an aqueous suspension by the addition of water generally contain the active ingredient together with a dispersing or wetting agent, suspending agent and one or more preservatives. Suitable dispersing or wetting agents and suspending agents are exemplified by those already mentioned above. Additional excipients such as sweetening, flavouring and colouring agents, may also be present.
The pharmaceutical compositions of the invention may also be in the form of oil-in- water emulsions. The oily phase may be a vegetable oil, such as olive oil or arachis oil, or a mineral oil, such as for example liquid paraffin or a mixture of any of these. Suitable emulsifying agents may be, for example, naturally-occurring gums such as gum acacia or gum tragacanth, naturally-occurring phosphatides such as soya bean, lecithin, an esters or partial esters derived from fatty acids and hexitol anhydrides (for example sorbitan monooleate) and condensation products of the said partial esters with ethylene oxide such as polyoxyethylene sorbitan monooleate. The emulsions may also contain sweetening, flavouring and preservative agents.
Syrups and elixirs may be formulated with sweetening agents such as glycerol, propylene glycol, sorbitol, aspartame or sucrose, and may also contain a demulcent, preservative, flavouring and/or colouring agent. The pharmaceutical compositions may also be in the form of a sterile injectable aqueous or oily suspension, which may be formulated according to known procedures using one or more of the appropriate dispersing or wetting agents and suspending agents, which have been mentioned above. A sterile injectable preparation may also be a sterile injectable solution or suspension in a non-toxic parenterally-acceptable diluent or solvent, for example a solution in 1,3-butanediol.
Compositions for administration by inhalation may be in the form of a conventional pressurised aerosol arranged to dispense the active ingredient either as an aerosol containing finely divided solid or liquid droplets. Conventional aerosol propellants such as volatile fluorinated hydrocarbons or hydrocarbons may be used and the aerosol device is conveniently arranged to dispense a metered quantity of active ingredient.
For further information on formulation the reader is referred to Chapter 25.2 in Volume 5 of Comprehensive Medicinal Chemistry (Corwin Hansch; Chairman of Editorial Board), Pergamon Press 1990.
The amount of active ingredient that is combined with one or more excipients to produce a single dosage form will necessarily vary depending upon the host treated and the particular route of administration. For example, a formulation intended for oral administration to humans will generally contain, for example, from 0.5 mg to 2 g of active agent compounded with an appropriate and convenient amount of excipients which may vary from about 5 to about 98 percent by weight of the total composition. Dosage unit forms will generally contain about 1 mg to about 500 mg of an active ingredient. For further information on Routes of Administration and Dosage Regimes the reader is referred to Chapter 25.3 in Volume 5 of Comprehensive Medicinal Chemistry (Corwin Hansch; Chairman of Editorial Board), Pergamon Press 1990.
The size of the dose for therapeutic or prophylactic purposes of a compound of the Formula (I), (IA), (IB), (IC) or (ID) will naturally vary according to the nature and severity of the conditions, the age and sex of the animal or patient and the route of administration, according to well known principles of medicine.
In using a compound of the Formula (I), (IA), (IB), (IC) or (ID) for therapeutic or prophylactic purposes it will generally be administered so that a daily dose in the range, for example, 0.5 mg to 75 mg per kg body weight is received, given if required in divided doses. In general lower doses will be administered when a parenteral route is employed. Thus, for example, for intravenous administration, a dose in the range, for example, 0.5 mg to 30 mg per kg body weight will generally be used. Similarly, for administration by inhalation, a dose in the range, for example, 0.5 mg to 25 mg per kg body weight will be used. Oral administration is however preferred.
The elevation of GLK activity described herein may be applied as a sole therapy or in combination with one or more other substances and/or treatments for the indication being treated. Such conjoint treatment may be achieved by way of the simultaneous, sequential or separate administration of the individual components of the treatment. Simultaneous treatment may be in a single tablet or in separate tablets. For example in the treatment of diabetes mellitus, chemotherapy may include the following main categories of treatment:
1) Insulin and insulin analogues;
2) Insulin secretagogues including sulphonylureas (for example glibenclamide, glipizide), prandial glucose regulators (for example repaglinide, nateglinide);
3) Agents that improve incretin action (for example dipeptidyl peptidase IV inhibitors, and GLP-I agonists); 4) Insulin sensitising agents including PPARgamma agonists (for example pioglitazone and rosiglitazone), and agents with combined PPARalpha and gamma activity;
5) Agents that modulate hepatic glucose balance (for example metformin, fructose 1, 6 bisphosphatase inhibitors, glycogen phopsphorylase inhibitors, glycogen synthase kinase inhibitors);
6) Agents designed to reduce the absorption of glucose from the intestine (for example acarbose);
7) Agents that prevent the reabsorption of glucose by the kidney (SGLT inhibitors); 8) Agents designed to treat the complications of prolonged hyperglycaemia (for example aldose reductase inhibitors);
9) Anti-obesity agents (for example sibutramine and orlistat);
10) Anti- dyslipidaemia agents such as, HMG-CoA reductase inhibitors (eg statins); PP ARa agonists (fibrates, eg gemfibrozil); bile acid sequestrants (cholestyramine); cholesterol absorption inhibitors (plant stanols, synthetic inhibitors); bile acid absorption inhibitors (IBATi) and nicotinic acid and analogues (niacin and slow release formulations);
11) Antihypertensive agents such as, β blockers (eg atenolol, inderal); ACE inhibitors (eg lisinopril); Calcium antagonists (eg. nifedipine); Angiotensin receptor antagonists (eg candesartan), α antagonists and diuretic agents (eg. furosemide, benzthiazide); 12) Haemostasis modulators such as, antithrombotics, activators of fibrinolysis and antiplatelet agents; thrombin antagonists; factor Xa inhibitors; factor Vila inhibitors); antiplatelet agents (eg. aspirin, clopidogrel); anticoagulants (heparin and Low molecular weight analogues, hirudin) and warfarin;
13) Agents which antagonise the actions of glucagon; and 14) Anti-inflammatory agents, such as non-steroidal anti-inflammatory drugs (eg. aspirin) and steroidal anti-inflammatory agents (eg. cortisone).
According to another aspect of the present invention there is provided individual compounds produced as end products in the Examples set out below and salts thereof.
A compound of the invention, or a salt thereof, may be prepared by any process known to be applicable to the preparation of such compounds or structurally related compounds. Functional groups may be protected and deprotected using conventional methods. For examples of protecting groups such as amino and carboxylic acid protecting groups (as well as means of formation and eventual deprotection), see T. W. Greene and P.G.M. Wuts, "Protective Groups in Organic Synthesis", Second Edition, John Wiley & Sons, New York, 1991.
Processes for the synthesis of compounds of Formula (I), (IA), (IB), (IC) or (ID) are provided as a further feature of the invention. Thus, according to a further aspect of the invention there is provided a process for the preparation of a compound of Formula (I), (IA), (IB), (IC) or (ID), which comprises a process a) to e) (wherein the variables are as defined hereinbefore for compounds of Formula (I), (IA), (IB), (IC) or (ID) unless otherwise defined):
(a) reaction of an acid of Formula (III) or activated derivative thereof with a compound of Formula (IV), wherein R1 is as hereinbefore defined or a protected version thereof;
Figure imgf000053_0001
(III) (IV); or (b) reaction of a compound of Formula (V) with a compound of Formula (VI),
Figure imgf000053_0002
(V) (VI) wherein X1 is a leaving group and X2 is a hydroxyl group or X1 is a hydroxyl group and X2 is a leaving group, and wherein R1 is as hereinbefore defined or a protected version thereof; process (b) could also be accomplished using the intermediate ester Formula (VII), wherein P1 is a protecting group as hereinafter described, followed by ester hydrolysis and amide formation by procedures described elsewhere and well known to those skilled in the art;
Figure imgf000054_0001
(V) (VII) or
(c) reaction of a compound of Formula (VIII) with a compound of Formula (IX)
Figure imgf000054_0002
(VIII) (IX) wherein X3 is a leaving group or an organometallic reagent and X4 is a hydroxyl group or X3 is a hydroxyl group and X4 is a leaving group or an organometallic reagent, and wherein R1 is as hereinbefore defined or a protected version thereof; process (c) could also be accomplished using the intermediate ester Formula (X), followed by ester hydrolysis and amide formation by procedures described elsewhere and well known to those skilled in the art;
Figure imgf000054_0003
(VIII) (X)
(d) reaction of a compound of Formula (XI) with a compound of Formula (XII),
Figure imgf000054_0004
(XI) (XII); wherein X5 is a leaving group; and wherein R1 is as hereinbefore defined or a protected version thereof; or e) reaction of a compound of formula (XIII)
Figure imgf000055_0001
(XIII) wherein R2a is a precursor to R2, such as a carboxylic acid, ester or anhydride (for R2 = -CONR4R5) or the sulfonic acid equivalents (for R2 is -SO2NR4R5); with an amine of formula -NR4R5; and thereafter, if necessary: i) converting a compound of Formula (I) into another compound of Formula (I); ii) removing any protecting groups; and/or iii) forming a salt thereof.
Suitable leaving groups X1 to X5 for processes b) to d) are any leaving group known in the art for these types of reactions, for example halo, alkoxy, trifluoromethanesulfonyloxy, methanesulfonyloxy, or p-toluenesulfonyloxy; or a group (such as a hydroxy group) that may be converted into a leaving group (such as an oxytriphenylphosphonium group) in situ.
Suitable values for R1 containing a protected hydroxy group are any suitable protected hydroxy group known in the art, for example simple ethers such as a methyl ether, tert-butyl ether or silylethers such as -OSi[(l-4C)alkyl]3 (wherein each (l-4C)alkyl group is independently selected from methyl, ethyl, propyl, isopropyl, and tertbutyl).
Examples of such trialkylsilyl groups are trimethylsilyl, triethylsilyl, triisopropylsilyl and tert-butyldimethylsilyl. Further suitable silyl ethers are those containing phenyl and substituted phenyl groups, such as -Si(PhMe2) and -Si(ToIMe2) (wherein ToI = methylbenzene). Further suitable values for hydroxy protecting groups are given hereinafter.
Compounds of Formulae (III) to (XII) are commercially available, or are known in the art, or may be made by processes known in the art, for example as shown in the accompanying Examples, or as described below. For further information on processes for making such- compounds, we refer to our PCT publications WO 03/000267, WO 03/015774 and WO 03/000262 and references therein. In general it will be appreciated that any aryl-0 or alkyl-0 bond may be formed by nucleophilic substitution or metal catalysed processes, optionally in the presence of a suitable base.
Compounds of Formula (XIII) may be made by processes such as those shown in processes a) to d) and/or by those processes mentioned above for compounds of formulae (III) to (XII).
The group R1 in the compounds of formulae (III), (IX), (X), (XI) and (XIII) may be made by reaction of suitable precursors with compounds of formula (V) or derivatives thereof, depending on the nature of the R1 group, for example, by nucleophilic displacement of a leaving group X1 in a compound of formula (V). Compounds of formula (V) are generally commercially available or maybe made by simple functional group interconversions from comercially available compounds, or by literature methods. Further information is available in WO2004/076420, WO2005/054200, WO2005/054233, WO 2005/044801 and WO 2005/056530. Some illustrative examples using various R1 groups are given in the Schemes below, and/or in the accompanying examples, and may generally be applied analogously to R1 groups not shown below by methods known in the art, see for example Bull. Chem. Soc. Japan, 73 (2000), 471-484, International Patent Application WO 2002/050003 and Bioorganic and Medicinal Chemistry Letters, (2001), 11, 407.
Figure imgf000056_0001
Scheme 1
Figure imgf000056_0002
Scheme 2
Figure imgf000057_0001
Scheme 3
Figure imgf000057_0002
CuI (1989),
Figure imgf000057_0003
Scheme 4
[PG is protecting group, Ts is p-toluenesulfonyl].
Examples of conversions of a compound of Formula (I) into another compound of Formula (I), well known to those skilled in the art, include functional group interconversions such as hydrolysis, hydrogenation, hydrogenolysis, oxidation or reduction, and/or further functionalisation by standard reactions such as amide or metal-catalysed coupling, or nucleophilic displacement reactions. Further examples of a conversion of a compound of Formula (I) into another compound of Formula (I) is methylation of a hydroxy group in R1 to give a methoxy group, and conversion of, for example, a hydroxymethyl group in R1 (such as when R1 is hydroxyprop-2-yl) into a difluoromethoxy group, using reactions such as those as illustrated in Scheme 4.
It will be understood that substituents R3, R6 and/or R7 may be introduced into the molecule at any convenient point in the synthetic sequence or may be present in the starting materials. A precursor to one of these substituents may be present in the molecule during the process steps a) to e) above, and then be transformed into the desired substituent as a final step to form the compound of formula (I); followed where necessary by i) converting a compound of Formula (I) into another compound of Formula (I); ii) removing any protecting groups; and/or iii) forming a salt thereof.
Specific reaction conditions for the above reactions are as follows, wherein when P1 is a protecting group P1 is preferably (l-4C)alkyl, for example methyl or ethyl: Process a) - coupling reactions of amino groups with carboxylic acids to form an amide are well known in the art. For example,
(i) using an appropriate coupling reaction, such as a carbodiimide coupling reaction performed with EDAC (l-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) in the presence of dimethylaminopyridine (DMAP) in a suitable solvent such as dichloromethane (DCM), chloroform or dimethylformamide (DMF) at room temperature; or
(ii) reaction in which the carboxylic group is activated to an acid chloride by reaction with oxalyl chloride in the presence of a suitable solvent such as DCM. The acid chloride can then be reacted with a compound of Formula (IV) in the presence of a base, such as triethylamine or pyridine, in a suitable solvent such as chloroform or DCM at a temperature between 0°C and 80°C.
Process b) - compounds of Formula (V) and (VI) can be reacted together in a suitable solvent, such as DMF or tetrahydrofuran (THF), with a base such as sodium hydride or potassium tert-butoxide, at a temperature in the range 0 to 2000C, optionally using microwave heating or metal catalysis such as palladium(II)acetate, palladium on carbon, copper(II)acetate or copper(I)iodide; alternatively, compounds of Formula (V) and (VI) can be reacted together in a suitable solvent, such as THF or DCM, with a suitable phosphine such as triphenylphosphine, and azodicarboxylate such as diethylazodicarboxylate; process b) could also be carried out using a precursor to the ester of formula (VII) such as an aryl-nitrile or trifluoromethyl derivative, followed by conversion to a carboxylic acid and amide formation as previously described; Process c) - compounds of Formula (VIII) and (IX) can be reacted together in a suitable solvent, such as DMF or THF, with a base such as sodium hydride or potassium tert-butoxide, at a temperature in the range 0 to 2000C, optionally using microwave heating or metal catalysis such as palladium(II)acetate, palladium on carbon, copper(II)acetate or copper(I)iodide; process c) could also be carried out using a precursor to the ester of formula (X) such as an aryl-nitrile or trifluoromethyl derivative, followed by conversion to a carboxylic acid and amide formation as previously described; compounds of the formula (VIII) are commercially available or can be prepared from commercially available materials by processes well known to those skilled in the art, for example functional group interconversions (such as hydrolysis, hydrogenation, hydrogenolysis, oxidation or reduction), and/or further functionalisation and/or cyclisation by standard reactions (such as amide or sulphonamide or metal-catalysed coupling, or nucleophilic displacement or electrophilic substitution reactions); Process d) - reaction of a compound of Formula (XI) with a compound of Formula (XII) can be performed in a polar solvent, such as DMF or a non-polar solvent such as THF wilh a strong base, such as sodium hydride or potassium fert-butoxide at a temperature between 0 and 2000C, optionally using microwave heating or metal catalysis, such as palladium(II)acetate, palladium on carbon, copper(II)acetate or copper(I)iodide; Process e) - coupling reactions of amino groups with carboxylic or sulfonic acids or acid derivatives to form an amide are well known in the art and are described above for Process a).
Certain intermediates of formula (III), (VI), (VII), (IX) and/or (XI) are believed to be novel and comprise an independent aspect of the invention.
Certain intermediates of formula (III), (IX) and/or (XI) wherein R1 is as defined herein for a compound of formula (I) are believed to be novel and comprise an independent aspect of the invention.
Certain intermediates of formula (XIII) are believed to be novel and comprise an independent aspect of the invention.
During the preparation process, it may be advantageous to use a protecting group for a functional group within the molecule. Protecting groups may be removed by any convenient method as described in the literature or known to the skilled chemist as appropriate for the removal of the protecting group in question, such methods being chosen so as to effect removal of the protecting group with minimum disturbance of groups elsewhere in the molecule. Specific examples of protecting groups are given below for the sake of convenience, in which "lower" signifies that the group to which it is applied preferably has 1-4 carbon atoms. It will be understood that these examples are not exhaustive. Where specific examples of methods for the removal of protecting groups are given below these are similarly not exhaustive. The use of protecting groups and methods of deprotection not specifically mentioned is of course within the scope of the invention.
A carboxy protecting group may be the residue of an ester-forming aliphatic or araliphatic alcohol or of an ester-forming silanol (the said alcohol or silanol preferably containing 1-20 carbon atoms). Examples of carboxy protecting groups include straight or branched chain (l-12C)alkyl groups (e.g. isopropyl, t-butyl); lower alkoxy lower alkyl groups (e.g. methoxymethyl, ethoxymethyl, isobutoxymethyl); lower aliphatic acyloxy lower alkyl groups, (e.g. acetoxymethyl, propionyloxymethyl, butyryloxymethyl, pivaloyloxymethyl); lower alkoxycarbonyloxy lower alkyl groups (e.g.
1-methoxycarbonyloxyethyl, 1-ethoxycarbonyloxyethyl); aryl lower alkyl groups (e.g. p_-methoxybenzyl, o-nitrobenzyl, β-nitrobenzyl, benzhydryl and phthalidyl); tri(lower alkyl)silyl groups (e.g. trimethylsilyl and t-butyldimethylsilyl); tri(lower alkyl)silyl lower alkyl groups (e.g. trimethylsilylethyl); and (2-6C)alkenyl groups (e.g. allyl and vinylethyl). Methods particularly appropriate for the removal of carboxyl protecting groups include for example acid-, metal- or enzymically-catalysed hydrolysis. Hydrogenation may also be used.
Examples of hydroxy protecting groups include methyl, t-butyl, lower alkenyl groups (e.g. allyl); lower alkanoyl groups (e.g. acetyl); lower alkoxycarbonyl groups (e.g. t-butoxycarbonyl); lower alkenyloxycarbonyl groups (e.g. allyloxycarbonyl); aryl lower alkoxycarbonyl groups (e.g. benzoyloxycarbonyl, rj-methoxybenzyloxycarbonyl, o-nitrobenzyloxycarbonyl, p_-nitrobenzyloxycarbonyl); tri lower alkyl/arylsilyl groups (e.g. trimethylsilyl, t-butyldimethylsilyl, t-butyldiphenylsilyl); tetrahydropyran-2-yl; aryl lower alkyl groups (e.g. benzyl) groups; and triaryl lower alkyl groups (e.g. triphenylmethyl). Examples of amino protecting groups include formyl, aralkyl groups (e.g. benzyl and substituted benzyl, e.g. β-methoxybenzyl, nitrobenzyl and 2,4-dimethoxybenzyl, and triphenylmethyl); di-p_-anisylmethyl and furylmethyl groups; lower alkoxycarbonyl (e.g. t-butoxycarbonyl); lower alkenyloxycarbonyl (e.g. allyloxycarbonyl); aryl lower alkoxycarbonyl groups (e.g. benzyloxycarbonyl, ρ_-methoxybenzyloxycarbonyl, o-nitrobenzyloxycarbonyl, p_-nitrobenzyloxycarbonyl; trialkylsilyl (e.g. trimethylsilyl and t-butyldimethylsilyl); alkylidene (e.g. methylidene); benzylidene and substituted benzylidene groups. Methods appropriate for removal of hydroxy and amino protecting groups include, for example, hydrogenation, nucleophilic displacement, acid-, base, metal- or enzymically-catalysed hydrolysis, catalytic hydrogenolysis or photolytically for groups such as o-nitrobenzyloxycarbonyl, or with fluoride ions for silyl groups. For example, methylether protecting groups for hydroxy groups may be removed by trimethylsilyliodide. A tert-butyl ether protecting group for a hydroxy group may be removed by hydrolysis, for example by use of hydrochloric acid in methanol.
Examples of protecting groups for amide groups include aralkoxymethyl (e.g. ben∑yloxymethyl and substituted ben2yloxymethyl); alkoxymethyl (e.g. methoxymethyl and trimethylsilylethoxymethyl); tri alkyl/arylsilyl (e.g. trimethylsilyl, t-butyldimethylsily, t-butyldiphenylsilyl); tri alkyl/arylsilyloxymethyl (e.g. t-butyldimethylsilyloxymethyl, t-butyldiphenylsilyloxymethyl); 4-alkoxyphenyl (e.g. 4-methoxyphenyl); 2,4- di(alkoxy)phenyl (e.g. 2,4-dimethoxyphenyl); 4-alkoxyben2yl (e.g. 4-methoxybenzyl); 2,4-di(alkoxy)benzyl (e.g. 2,4-di(methoxy)benzyl); and alk-1-enyl (e.g. allyl, but-1-enyl and substituted vinyl e.g. 2-phenylvinyl).
Aralkoxymethyl, groups may be introduced onto the amide group by reacting the latter group with the appropriate aralkoxymethyl chloride, and removed by catalytic hydrogenation. Alkoxymethyl, tri alkyl/arylsilyl and tri alkyl/silyloxymethyl groups may be introduced by reacting the amide with the appropriate chloride and removing with acid; or in the case of the silyl containing groups, fluoride ions. The alkoxyphenyl and alkoxybenzyl groups are conveniently introduced by arylation or alkylation with an appropriate halide and removed by oxidation with eerie ammonium nitrate. Finally alk-1- enyl groups may be introduced by reacting the amide with the appropriate aldehyde and removed with acid. In the above other pharmaceutical composition, process, method, use and medicament manufacture features, the alternative and preferred aspects and embodiments of the compounds of the invention described herein also apply.
The following examples are for illustration purposes and are not intended to limit the scope of this application. Each exemplified compound represents a particular and independent aspect of the invention. In the following non-limiting Examples, unless otherwise stated: (i) evaporations were carried out by rotary evaporation in vacuo and work-up procedures were carried out after removal of residual solids such as drying agents by filtration;
(ii) operations were carried out at room temperature, that is in the range 18-25°C and under an atmosphere of an inert gas such as argon or nitrogen;
(iii) yields are given for illustration only and are not necessarily the maximum attainable;
(iv) the structures of the end-products of the Formula (I) were confirmed by nuclear (generally proton) magnetic resonance (NMR) with a field strength (for proton) of 300MHz (generally using a Varian Gemini 2000) or 400 MHz (generally using a Bruker Avance DPX400), unless otherwise stated, and mass spectral techniques; proton magnetic resonance chemical shift values were measured on the delta scale and peak multiplicities are shown as follows: s, singlet; d, doublet; t, triplet; m, multiplet; br, broad; q, quartet, quin, quintet; (v) intermediates were not generally fully characterised and purity was assessed by thin layer chromatography (TLC), high-performance liquid chromatography (HPLC), infra-red (IR) or NMR analysis;
(vi) Purification by chromatography generally refers to flash column chromatography, on silica unless otherwise stated. Column chromatography was generally carried out using prepacked silica cartridges (from 4g up to 40Og) such as Redisep™
(available, for example, from Presearch Ltd, Hitchin, Herts, UK) or Biotage (Biotage UK Ltd, Hertford, Herts, UK), eluted using a pump and fraction collector system. Purification by Solid Phase Extraction (SPE) methods generally refers to the use of chromatography cartridges packed with SPE materials such as ISOLUTE® SCX-2 columns (available, for example, From International Sorbent Technology Ltd, Dryffryn Business Park, Hengoed, Mid Glamorgan, UK);
(vii) Mass spectra (MS) data was generated on an LCMS system where the HPLC component comprised generally either a Agilent 1100 or Waters Alliance HT (2790 & 2795) equipment and was run on a Phemonenex Gemini Cl 8 5μm, 50 x 2 mm column (or similar) eluting with either acidic eluent (for example, using a gradient between 0 - 95% water / acetonitrile with 5% of a 1% formic acid in 50:50 water: acetonitrile (v/v) mixture; or using an equivalent solvent system with methanol instead of acetonitrile), or basic eluent (for example, using a gradient between 0 - 95% water / acetonitrile with 5% of a 0.1% 880 Ammonia in acetonitrile mixture); and the MS component comprised generally a Waters ZQ spectrometer. Chromatograms for Electrospray (ESI) positive and negative Base Peak Intensity, and UV Total Absorption Chromatogram from 220-3 OOnm, are generated and values for m/z are given; generally, only ions which indicate the parent mass are reported and unless otherwise stated the value quoted is (M-H)";
(viii) Suitable microwave reactors include "Smith Creator", "CEM Explorer", "Biotage Initiator sixty" and "Biotage Initiator eight".
(ix) Melting points were generally carried out by Differential Scanning Calorimetry (DSC); analysis was generally conducted using equipment such as a Mettler DSC822e or a Mettler DSC820. Samples of typically less than 5 mg of material, contained in a 40 μL aluminum pan fitted with a pierced lid, were heated over the temperature range 25 °C to 325°C at a constant heating rate of 1O0C per minute. A purge gas using nitrogen was used at a flow rate of 100 rnL per minute. It will be understood that the onset and/or peak temperature values of the DSC may vary slightly from one machine to another, one method to another or from one sample to another, and so the values quoted are not to be construed as absolute. It will be appreciated that some samples may be solvates and that this may also affect melting points.
Abbreviations
DCM dichloromethane
DEAD diethylazodicarboxylate
DIAD diisopropylazodicarboxylate
DIPEA N,N-Diisopropylethylamine DMA dimethylacetamide
DMSO dimethyl sulphoxide
DMF dimethylformamide
EDAC l-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride HATU O-(7-Azabenzotriazol-l-yl)-Ν,Ν,Ν',Ν'- tetramethyluronium hexofluorophosphate
HPLC high pressure liquid chromatography HPMC Hydroxypropylmethylcellulose
LCMS liquid chromatography / mass spectroscopy
NMP N-methyl-2-pyrrolidone
ΝMR nuclear magnetic resonance spectroscopy RT room temperature
THF tetrahydrofuran
TFA trifluoroacetic acid
CDCl3 deuterochloroform
MgSO4 magnesium sulfate ΝaHMDS sodium hexamethyldisilazide
All compound names were derived using ACD NAME computer package.
Example 1; 3-(f5-(Azetidin-l-ylcarbonvnpyridin-2-ylloxy>-5-{[(lS)-2-hvdroxy-l- methylethylloxy}-iV-l,3-thiazol-2-ylbenzamide
Figure imgf000064_0001
A mixture of 3-{[5-(azetidin-l-ylcarbonyl)pyridin-2-yl]oxy}-5-[((15)-2-{[(l,l- dimethylethyl)(dimethyl)silyl]oxy}-l-methylethyl)oxy]-N-l,3-thiazol-2-ylbenzamide (0.15 g, 0.33 mmol) in methanol (10 mL) and IM hydrochloric acid (10 mL) was stirred for 90 mins at RT. The volatiles were removed in vacuo and the residue taken to pH6 with saturated aqueous sodium bicarbonate solution then extracted into ethyl acetate (3 x 30 mL) and the combined organic layers washed with water (30 mL), brine (30 mL), dried (MgSO4), filtered and the solvents removed in vacuo to a residue which was chromatographed on silica eluting with a gradient of 0-10% methanol in ethyl acetate to give the desired compound (65 mg).
1R ΝMR δ (CDCl3): 1.32 (d, 3H), 2.30 (s, IH), 2.39 (quin, 2H), 3.79 (m, 2H), 4.20-4.40 (brm, 4H), 4.58 (m, IH), 6.98 (m, 2H), 7.27 (m, IH), 7.35 (m, IH), 7.39 (s, IH), 7.41 (s, IH), 8.11 (d, IH), 8.41 (s, IH), 11.50 (s, IH). ?n/z 455 (M+H)+ The following compounds were synthesised in an analogous fashion from the appropriate silyl ether.
Figure imgf000065_0001
The preparation of 3-{ [5-(azetidin- 1 -ylcarbonyl)pyridin-2-yl]oxy }-5-[((liS)-2-{ [(1 , 1 - dimethylethyl)(dimethyl)silyl]oxy}-l-methylethyl)oxy]-N-l,3-thiazol-2-ylbenzamide used in Example 1 is described below: 3-ir5-fAzetidm-l-ylcarbαnyl>pyridin-2-ylloxy>-5-rffl-^-2-(rfl.l- dimethylethvDCdimethvπsilyll oxy 1-1 -methylethvDoxyi -N- 1 ,3-thiazol-2-ylbenzamide
Figure imgf000066_0001
Potassium carbonate (0.14 g, 0.98 mmol) was added to a mixture of 3-[((15)-2-{[(l,l- dimethylethyl)(dimethyl)silyl] oxy } - 1 -methylethyl)oxy] -5-hydroxy-N- 1 ,3 -thiazol-2- ylbenzamide (200 mg, 0.49 mmol) and 5-(azetidin-l-ylcarbonyl)-2-chloropyridine (96 mg, 0.49 mmol) in acetonitrile (5.0 mL) and the stirred mixture heated at 16O0C in a 'Biotage initiator Microwave' for 4 hours. The mixture was allowed to reach RT and pressure and was partitioned between ethyl acetate (50 mL) and water (50 mL). The ethyl acetate layer was separated, washed with water (5 x 50 mL), brine (50 mL), dried (MgSO4) and evaporated to a residue which was chromatographed on silica, eluting with a gradient of 60-100% ethyl acetate in isohexane, to give the desired compound. (0.15 g). m/z 569 (M+H)+
The precursor for Example Ia was prepared in a similar fashion from 3-[((liS)-2-{[(l,l- dimethylethyl)(dimethyl)silyl]oxy } - 1 -methylethyl)oxy]-5-hydroxy-N- 1 ,3-thiazol-2- ylbenzamide using 5-(azetidin-l-ylcarbonyl)-2,3-dichloropyridine:
Figure imgf000066_0002
The precursor for Example Ib was prepared as described below: 3-{[6-(Azetidin-l-ylcarbonvnpyridin-3-yl1oxyl-5-rr(l^-2-irn.l- dimethylethviydimethvπsilylloxy)-l-methylethyl)oxy1-A/-l,3-thiazol-2-ylbenzamide
Figure imgf000067_0001
Cesium carbonate (0.478 g, 1.47 mmol) was added to a mixture of 3-[((l£)-2-{[(l,l- dimethylethyl)(dimethyl)silyl]oxy}-l-methylethyl)oxy]-5-hydroxy-N-l,3-thiazol-2- ylbenzamide (200 mg, 0.49 mmol), bromotris(triphenylphosphine)copper (46 mg, 0.049 mmol) and 2-(azetidin-l-ylcarbonyl)-5-bromopyridine (130 mg, 5.39 mmol) in DMA (5.0 niL) and the stirred mixture heated at 16O0C in a 'Biotage initiator Microwave' for 3 hours.
The mixture was allowed to reach RT and pressure and was partitioned between ethyl acetate (50 mL) and water (50 mL). The ethyl acetate layer was separated, washed with water (5 x 50 mL), brine (50 mL), dried (MgSO4) and evaporated to a residue which was chromatographed on silica, eluting with a gradient of 0-100% ethyl acetate in isohexane, to give the desired compound. (47 mg).
1H ΝMR δ (CDCl3): 0.00 (s, 3H), 0.03 (s, 3H), 0.83 (s, 9H), 1.29 (d, 3H), 2.32 (quin, 2H), 3.65-3.80 (m, 2H), 4.21 (t, 2H), 4.46 (m, IH), 4.68 (t, 2H), 6.81 (s, IH), 6.92 (m, IH), 7.25
(m, IH), 7.30-7.50 (m, 2H), 7.65 (m, IH), 8.08 (d, IH), 8.28 (s, IH), 11.50 (brs, IH). m/z 569 (M+H)+
The precursors for examples Ic-Id were prepared in a similar fashion from 3-hydroxy-N- (l-methyl-lH-pyrazol-3-yl)-5-[((15)-l-methyl-2-{ [tris(l- methylethyl)silyl]oxy}ethyl)oxy]benzamide using the appropriate bromopyridine:
Figure imgf000068_0001
Bromotris(triphenylphosphine)copper may be prepared according to Synthetic Communications, 31(18), 2865-2879 (2001). The preparation of bromotris(triphenylphosphine)copper is described below.
Bromotris(triphenylphosphine)copper
Copper (II) bromide (111.7 g, 0.5 mol) was added in portions to a stirred solution of triphenylphosphine (557.4 g, 2.13 mol) in methanol (1.85 L) at RT (an exotherm to 320C was observed). The reaction was stirred for 10 minutes then heated to 650C and cooled overnight. The solid was collected by filtration, washed with ethanol (5 vol), diethyl ether (3 x 5 vol), dried on sinter under vacuum for 2 hours, then in a vacuum oven at 3O0C overnight to give product as crystalline solid (460 g).
The preparation of 5-(azetidin-l-ylcarbonyl)-2-chloropyridine used in the preparation of 3- { [5-(azetidin- 1 -ylcarbonyl)ρyridin-2-yl]oxy } -5-[(( lS)-2- {[(1,1- dimethylethyl)(dimethyl)silyl]oxy}-l-methylethyl)oxy]-N-l,3-thiazol-2-ylbenzamide, a precursor to Example 1, is described below:
5 -(Azetidin- 1 -ylcarbonyl)-2-chloropyridine r^Ycl DMF (2 drops) was added to a solution of 6-chloronicotinic acid (1.00 g, 6.35 mmol) and oxalyl chloride (0.67 mL, 7.62 mmol) in DCM (20 mL) and 2M hydrogen chloride in ether (3.15 mL, 6.35 mmol). The mixture was stirred at RT for 4 hours and the DCM and excess oxalyl chloride evaporated in vacuo. The residual acid chloride was dissolved in DCM (10 mL) and added to a mixture of azetidine hydrochloride (0.66 g, 6.99 mmol) and triethylamine (2.14 mL, 13.97 mmol) in DCM (10 mL) then stirred at RT for 24 hours. The DCM was evaporated in vacuo, and the residue partitioned between ethyl acetate (100 mL) and IM citric acid (50 mL). The ethyl acetate layer was washed sequentially with saturated aqueous sodium hydrogen carbonate (50 mL) and brine (50 mL), dried (MgSO4), and the solvent removed in vacuo to give a residue which was chromatographed on silica, eluting with a gradient of 50-100% ethyl acetate in isohexane, to give the desired compound (0.73 g). 1R NMR δ (CDCl3) 2.43 (quin, 2H), 4.2-4.4 (brm, 4H), 7.42 (d, IH), 7.97 (d, IH), 8.63 (s, IH). m/z 197 (M+H)+
The halopyridines used in the preparation of precursors for Examples la-Id were prepared in a similar fashion from the appropriate nicotinic acid:
Figure imgf000069_0001
The nicotinic acids were either commercially available compounds or compounds known in the literature. Reference for 2-(azetidin-l-ylcarbonyl)-5-bromopyridine (Heterocycles, 15(1) 1981, 213-23). The preparation of 3-[((15)-2-{ [(I , l-dimethylethyl)(dimethyl)silyl]oxy}- 1 - methylethyl)oxy]-5-hydroxy-N-l,3-thiazol-2-ylbenzamide used in the synthesis of Example 1, Ia and Ib is described below:
B-rrπ^-Σ-irriJ-DimethylethviydimethvDsilylioxyl-l-methylethvDoxyl-S-hvdroxy-iV- 1 ,3-thiazol-2-ylbenzamide
Figure imgf000070_0001
An aqueous solution of lithium hydroxide monohydrate (0.304 g, 7.25 mmol) was added to a solution of 3-{ [(1 , 1 -dimethylethyl)(dimethyl)silyl]oxy } -5-[((lS)-2-{ [(1 , 1 - dimethylethyl)(dimethyl)silyl]oxy}- 1 -methylethyl)oxy]-JV- 1 ,3-thiazol-2-ylbenzamide
(1.552 g, 2.90 mmol) in THF (40 mL) and stirred at RT overnight. The THF was removed in vacuo and the residual solution adjusted to pH 7 by addition of IN hydrochloric acid (7.25 mL). A gum formed, and the solution was filtered and the gum dissolved in methanol. The filtrate was partitioned with ethyl acetate (75 mL) and the organics washed with brine, dried (MgSO4), combined with the methanol solution and evaporated to a residue which was chromatographed on silica, eluting with 40% ethyl acetate in isohexane, to give the required product (0.865 g)
1E ΝMR 6 (CDCl3): -0.02 (d, 6H), 0.81 (s, 9H), 1.24 (d, 3H), 3.67 (m, 2H), 4.43 (sextet, IH), 6.65 (t, IH), 7.00 (d, IH), 7.13 (t, IH), 7.15 (t, IH), 7.40 (d, IH). m/z 409 (M+H)+
3-1 |Y1 ■ l-DimethylethviydimemylMylloxyV5-r(Tl^-2-{ ITl .1- dimethylethyl)(dimethyl)silvnoxy}-l-methylethyl')oxyl-N-L3-thiazol-2-ylbenzamide
tert-Butyldimethylsilyl chloride (1.66 g, 11.0 mmol) and imidazole (1.78 g, 26.0 mmol) were a to a solution of 3-hydroxy-5-{[(liS)-2-hydroxy-l-methylethyl]oxy}-N-l,3-thiazol-2- ylbenzamide (1.541 g, 5.24 mtnol)in DMF (10 mL) and the reaction stirred at RT under a silica drying tube overnight. Water (30 mL) was added and the solution partitioned with ethyl acetate (100 mL). The organic phase was washed with brine, dried (MgSO4) and evaporated to a residue which was chromatographed on silica, eluting with 15% ethyl acetate in isohexane, to give the required product (1.525 g). 1H NMR δ (CDCl3): -0.17 (d, 6H), 0.00 (s, 6H), 0.65 (s, 9H), 0.76 (s, 9H), 1.08 (d, 3H), 3.50 (m, 2H), 4.25 (sextet, IH), 6.43 (t, IH), 6.77 (d, IH), 6.83 (t, IH), 6.96 (t, IH), 7.06 (d, IH). m/z 423 (M-H)"
3 -Hydroxy-5- { IY 1 ■Sr)-2-hvdroxy- 1 -methylethyli oxy } -N- 1 ,3 -thiazol-2-ylbenzamide
Figure imgf000071_0001
Iodo(trimethyl)silane (7.19 mL, 50.5 mmol) was added dropwise to a solution of 3- hydroxy-5-{[(lJS)-2-methoxy-(l-methylethyl)oxy}-N-l,3-thiazol-2-ylbenzamide (3.116 g, 10.1 mmol) in acetonitrile (60 mL) under an inert atmosphere and the reaction stirred at RT overnight. Methanol (5 mL) was added and the reaction stirred a further 10 minutes. Saturated sodium thiosulphate solution (5 mL) and saturated potassium carbonate solution (5 mL) were added and the reaction stirred a further 10 minutes. The organic solvents were removed in vacuo and the resulting solution partitioned extracted with ethyl acetate (100 mL). The Aqueous layer was adjusted to pH 5 with the addition of IM hydrochloric acid and extracted with ethyl acetate. The combined organic layers were washed with brine (20 mL), dried (MgSO4), and evaporated to a residue which was chromatographed on silica, eluting with ethyl acetate, to give the desired compound (1.541 g).
1H ΝMR δ (dβ-DMSO): 1.23 (d, 3H), 3.52 (m, 2H), 4.50 (sextet, IH), 6.55 (t, IH), 7.04 (t, IH), 7.18 (t, IH), 7.27 (d, IH), 7.55 (d, IH), 12.47 (s, IH). m/z 295 (M+H)+
3-Hydroxy-5-{rrity)-2-methoxy-ri-methylethyl)oxy}-N-L3-thiazol-2-ylbenzamide
Figure imgf000071_0002
A solution of 3-{[(15)-2-methoxy-(l-methylethyl)oxy}-5-{[(2-methylphenyl)methyl]oxy}- N-l,3-thiazol-2-ylbenzamide (6.9 g) and thioanisole (10 mL) in trifiuoroacetic acid (65 mL) was stirred at ambient temperature for 16 hours. The trifluoroacetic acid was removed in vacuo and the residual oil partitioned between ethyl acetate (75 mL) and aqueous sodium hydrogen carbonate solution (200 mL). The aqueous layer was separated, extracted with ethyl acetate (2 x 75 mL), and the combined organic extracts washed with brine, dried (MgSO4), and evaporated to a residue which was chromatographed on silica with 50% ethyl acetate in isohexane as eluant to give the desired compound (4.6 g). 1H NMR δ (CDCl3): 1.3 (d, 3H), 3.4 (s, 3H), 3.5-3.6 (m, 2H), 4.5-4.6 (m, IH), 6.65 (s, IH), 6.95 (d, IH), 7.05 (s, IH), 7.1 (s, IH)5 7.25 (d, IH). m/z 309 (M+H)+
3-{rα^)-2-Methoxy-ri-methylethvnoxy)-5-{rα-methylphenvnmethylloxy)-N-1.3- thiazol-2-ylbenzamide
Figure imgf000072_0001
To a solution of 3-[(15)-2-methoxy-(l-methylethyl)oxy]-5-{[(2- methylphenyl)methyl]oxy} benzoic acid (9.55 g) in DCM (140 mL) was added oxalyl chloride (2.83 mL), followed by DMF (1 drop), and the mixture stirred at ambient temperature for 16 hours. The DCM and excess oxalyl chloride were removed in vacuo, the residual oil dissolved in DCM (25 mL) and added to a solution of 2-aminothiazole (2.84g) and triethylamine (7.88 mL) in DCM (75 mL) at 0-50C, and the mixture stirred at ambient temperature for 4 hours. The DCM and excess triethylamine were removed in vacuo, the residual oil partitioned between ethyl acetate (100 mL) and IM hydrochloric acid (100 mL). The ethyl acetate layer was separated, washed sequentially with IM hydrochloric acid, aqueous sodium hydrogen carbonate solution, and brine, dried (MgSO4), and evaporated to a residue which was chromatographed on alumina with ethyl acetate as eluant to give the desired compound (11.0 g). 1H ΝMR δ (CDCl3): 1.3 (d, 3H), 2.35 (s,3H), 3.4 (s, 3H), 3.5-3.6 (m, 2H), 4.55-4.6 (m, IH), 5.0 (s,2H), 6.8 (s, IH), 6.95 (d, IH), 7.15 (s, IH), 7.25 (m, 5H), 7.4 (d, IH). m/z 413 (M+H)+ 3-rd^-2-Methoxy-d-methylethvDoxy1-5-{r('2-methylphenyl)niethylloxylbenzoic acid
Figure imgf000073_0001
A solution of methyl 3-[{(l»S)-2-methoxy-(l-methylethyl)oxy]-5-{[(2-methylphenyl) methyl] oxy}benzoate (10.65g) in THF (200 mL) and methanol (50 mL) was added to a solution of lithium hydroxide monohydrate (6.0g) in water (100 mL). The mixture was stirred at ambient temperature for 16 hours and the THF and methanol removed in vacuo. The aqueous layer was acidified to pHl with hydrochloric acid, and extracted with ethyl acetate (3 x 50 mL). The combined organic extracts were washed with brine, dried (MgSO4), and evaporated to give the desired compound (9.55 g). m/z 329 (M-H)"
Methyl 3-πri>y)-2-methoxy-α-methylethvπoxy1-5-(rr2-methylt)henvπmethylloxy> benzoate
Figure imgf000073_0002
A stirred suspension of methyl 3-hydroxy-5-{[(2-methylphenyl)methyl]oxy}benzoate (15.3 g) and polymer-supported triphenyl phosphine (39.2 g) in dry DCM (900 mL) was cooled in an ice-bath and diisopropyl azodicarboxylate (11.88 mL) was added drop wise. The reaction mixture was stirred at 0-50C for 30 minutes and (/<!)- l-methoxy-propan-2-ol was added dropwise. The reaction mixture was stirred at ambient temperature for 16 hours, filtered through diatomaceous earth and the DCM evaporated to a residue which was chromatographed on silica with 10% ethyl acetate in isohexane as eluant to give the desired compound (10.7 g). 1HNMR δ (CDCl3): 1.3 (d, 3H), 2.4 (s,3H), 3.4 (s, 3H), 3.5- 3.6 (m, 2H), 3.9 (s, 3H), 4.55-4.6 (m, IH), 5.0 (s,2H), 6.8 (s, IH), 7.25 (m, 5H), 7.4 (d, IH) Methyl 3-hydroxy-5-{ r(2-memylphenyl)methyl]oxy}benzoate
Figure imgf000074_0001
To a solution of methyl 3,5-dihydroxybenzoate (50 g, 0.30 mol) in DMF (500 mL) at 00C was added sodium hydride (10.8 g, 0.27 mol) portionwise, maintaining the reaction temperature below 100C. The reaction was allowed to warm to 15°C, and was stirred for 20 minutes. The mixture was cooled to O0C and a solution of 2-methylbenzyl bromide (36 mL, 0.27 mol) in DMF (50 mL) was added over 30 minutes. The reaction was warmed to ambient temperature and concentrated in vacuo, the residual oil partitioned between ethyl acetate (500 mL) and water (250 mL), the ethyl acetate layer separated, washed sequentially with water and brine, dried (MgSO4) and evaporated to a residue which was chromatographed on silica eluting with a gradient of 0-100% ethyl acetate in isohexane to give the desired compound (21.9 g). 1H NMR δ (CDCl3) 2.39 (s, 3H), 3.90 (s, 3H), 5.02 (s, 2H), 5.61 (s, IH), 6.69 (t, IH), 7.15-7.42 (m, 6H).
The preparation of 3-hydroxy-iV-(l-methyl-lH-pyrazol-3-yl)-5-[((15)-l-methyl-2-{[tris(l- methylethyl)silyl]oxy}ethyl)oxy]benzamide used in the synthesis of Example Ic-Id is described below:
3-Ηvdroxy-N-fl-methyl-lH-pyrazol-3-ylV5-rrn^-l-methyl-2-{rtris(l- methylethvDsilylloxylethvDoxylbenzamide
Figure imgf000074_0002
10% Palladium on carbon was added to 3-(benzyloxy)-N-(l-methyl-lH-pyrazol-3-yl)-5- {(15)-l-methyl-2-[(triisopropylsilyl)oxy]ethoxy}benzamide (21.7 g, 40.4 mmol) in dry TΗF (480 mL) under argon. The reaction mixture was degassed and placed under a hydrogen balloon and stirred for 16 hours. The atmosphere was replaced with argon and mixture was filtered through diatomaceous earth then the filtrate evaporated and dried under high vacuum for 1 hour to give the title compound (18.2 g). 1H NMR δ (CDCl3): 1.05 (s, 18H), 1.05-1.1 (m, 3H), 1.3 (d, 3H), 3.7 (m, IH), 3.8 (s, 3H), 3.9 (m, IH), 4.5 (m, IH), 6.6 (s, IH), 6.8 (s, IH), 7.0 (m, 2H), 7.20 (s, IH), 7.3 (s, IH), 8.7 (s, IH). m/z 448 (M+H)+, 446 (M-H)"
3-rBenzyloxyVN-ri-methyl-lH-pyrazol-3-ylV5-{fl^-l-methyl-2- [(triisopropylsilvDoxyiethoxyibenzamide
Figure imgf000075_0001
ΗATU (23.5 g, 61.8 mmol) was added to 3-(benzyloxy)-5-{(l,S)-l-methyl-2-
[(triisopropylsilyl)oxy]ethoxy}benzoic acid (23.6 g, 51.5 mmol), followed by addition of DMF (140 mL), and cooled to 0°C. 3-Amino-l-methylρyrazole (6.00 g. 61.8 mmol) was added followed by DIPEA (21.3 mL) and the reaction was stirred under argon at 0°C for 3 hours. The solvent was evaporated and the residue was dissolved in ethyl acetate (500 mL) and washed with citric acid solution (200 mL), sodium hydrogen carbonate solution (150 mL), and saturated brine solution (2 x 15OmL). The organic layer was separated and dried (MgSO4), filtered and evaporated. Purification by column chromatography, eluting with 1:4 to 1:1 ethyl acetate:hexanes, afforded the title compound as a colourless oil (21.7g). 1H NMR o (CDCl3): 1.05 (s, 18H), 1.05-1.1 (m, 3H), 1.3 (d, 3H), 3.7 (m, IH), 3.8 (s, 3H), 3.9 (m, IH), 4.5 (m, IH), 5.1 (s, 2H), 6.7 (s, IH), 6.8 (s, IH), 7.0 (m, 2H), 7.1 (s, IH), 7.3 (s, IH), 7.35 -7.5 (m, 5H), 8.5 (s, IH). m/z 538 (M+H)+ S-CBenzyloxyVS-iπ^-l-meihyl-Σ-rftriisopropylsilvDoxylethoxylbenzoic acid
Figure imgf000076_0001
Lithium hydroxide monohydrate (12.14 g, 0.289 mol) in water (100 mL) was added to a solution of methyl 3-(benzyloxy)-5-{(l>S)-l-methyl-2- [(triisopropylsilyl)oxy]ethoxy}benzoate (62 g, 0.131 mol) in THF (300 mL) and warmed to
430C. The reaction was stirred for 16 hours, the THF removed in vacuo and the resultant mixture acidified to pH 5 with 10% w/v citric acid. This was extracted with ethyl acetate (2 x 300 mL) and the combined organic layers were dried (MgSO4), filtered and evaporated to afford the title compound (60.2 g). 1H NMR δ (CDCl3): 1.05 (s, 18H), 1.05-1.1 (m, 3H), 1.35 (d, 3H), 3.7 (m, IH), 3.9 (m,
IH), 4.5 (m, IH)5 5.1 (s, 2H), 6.8 (s, IH), 7.3 -7.5 (m, 7H). m/z 457 (M-H)'
Methyl 3-rbenzyloxy)-5-{(l>Sf)-l-methyl-2-rrtriisor)roϋylsilvDoxylethoxy}benzoate
Figure imgf000076_0002
(2i?)-l-[(Triisopropylsilyl)oxy]propan-2-ol (56.1 g, 242 mmol) was added to a solution of methyl 3-hydroxy-5-{[phenylmethyl]oxy}benzoate (50 g, 194 mmol) and triphenylphosphine (63.5 g, 242 mmol) in dry THF (500 mL), at to O0C, followed by addition of DIAD (47.6 mL, 242 mmol) over 45 minutes under an argon atmosphere. The reaction was stirred at O0C for 1 hour and allowed to warm up to RT over an hour then stirred at RT for 1 hour. The THF was evaporated and a mixture of ethyl acetate (80 mL) and hexane (120 mL) was added. This mixture stirred for 2 hours and filtered. The precipitate was washed with a mixture of ethyl acetate (20 mL) and hexane (180 mL) and the filtrate evaporated. The residue was purified by column chromatography, eluting with 1:20 to 1:10 ethyl acetate:hexanes, to afford the title compound (65.5 g). 1E NMR δ (CDCl3): 1.05 (s, 18H), 1.05-1.1 (m, 3H), 1.35 (d, 3H), 3.7 (m, IH), 3.9 (m, IH), 3.9 (s, 3H), 4.5 (m, IH), 5.05 (s, 2H), 6.75 (s, IH), 7.2 (s, IH). 7.3 -7.5 (m, 6H). m/z 471 (M-H)"
( 2R)- 1 - rrrriisopropylsilvDoxyipropan-2-ol
Figure imgf000077_0001
Triisopropylsilyl chloride (83.8 mL, 390 mmol) was added slowly over 15 minutes to a solution of (2i?)-proρane-l,2-diol (29.7 g, 390 mmol) in DMF at 00C (100 mL) keeping the internal temperature below 150C. This was followed by addition of imidazole (66.4 g, 975 mmol) and the reaction mixture was allowed to warm to RT and stirred under argon for 20 hours. The reaction was quenched with IM hydrochloric acid/diethyl ether (300 mL/800 mL). The organic layer was separated and washed with IM hydrochloric acid followed by saturated brine solution. The organic layer was dried (MgSO4), filtered and evaporated. Purification by distillation at lOmmHg, 90-104°C, afforded the title compound as colourless oil (69.5 g). 1H NMR δ (CDCl3): 1.05 (s, 18H), 1.05-1.1 (m, 3H), 1.05 (d, 3H), 2.55 (s, IH), 3.45 (dd, IH), 3.7 (dd, IH), 3.85 (m, IH).
Methyl 3-hvdroxy-5-{ Tphenylmethylioxylbenzoate
Figure imgf000077_0002
To a stirred solution of methyl 3,5-dihydroxybenzoate (5.95 mol) in DMF (6 L) was added potassium carbonate (9 mol), and the suspension stirred at ambient temperature under argon. To this was added benzyl bromide (8.42 mol) slowly over 1 hour, with a slight exotherm, and the reaction mixture stirred overnight at ambient temperature. The reaction was quenched cautiously with ammonium chloride solution (5 L) followed by water (35 L). The aqueous suspension was extracted with DCM (1 x 3 L and 2 x 5 L). The combined extracts were washed with water (10 L) and dried overnight (MgSO4). The solution was evaporated in vacuo; and the crude product chromatographed in 3 batches (flash column, 3 x 2 kg silica, eluting with a gradient consisting of hexane containing 10% DCM, to neat DCM, to DCM containing 50% ethyl acetate) to eliminate starting material. The crude eluant was further chromatographed in 175 g batches (Amicon HPLC, 5 kg normal-phase silica, eluting with isohexane containing 20% v/v of ethyl acetate) to give the desired compound (21% yield). 1H NMR δ (d6-DMSO): 3.8 (s, 3H), 5.1 (s, 2H), 6.65 (m, IH), 7.0 (m, IH), 7.05 (m, IH), 7.3-7.5 (m, 5H), 9.85 (br s, IH).
Example 2; S-lfS-fAzetidin-l-ylcarbonvDpyridin^-ylloxyl-S-ird^-l-methyl-l- (methyloxy)ethylloxyl-N-(l-methyl-liy-pyrazol-3-yl)benzamide
Figure imgf000078_0001
Potassium carbonate (0.181 g, 1.31 mmol) was added to a mixture of 3-hydroxy-5-[(15)-2- methoxy-(l-methylethyl)oxy]-N-(l-methyl-lH-pyrazol-3-yl)benzamide (0.2 g, 0.66 mmol) and 5-(azetidin-l-ylcarbonyl)-2-chloropyridine (129 mg, 0.66 mmol) in acetonitrile (5.0 mL) and the stirred mixture heated at 16O0C in a 'Biotage initiator Microwave' for 6 hours. The mixture was allowed to reach RT and pressure and was partitioned between ethyl acetate (50 mL) and water (50 mL). The ethyl acetate layer was separated, washed with water (5 x 50 mL) brine (50 mL), dried (MgSO4) and evaporated to a residue which was chromatographed on silica, eluting with a gradient of 0-5% methanol in ethyl acetate, to give the desired compound (202 mg). 1H ΝMR δ (CDCl3): 1.33 (d, 3Η), 2.38 (quin, 2H), 3.40 (s, 3H)3 3.61 - 3.47 (m, 2H), 3.74 (s, 3H), 4.42 - 4.18 (m, 4H), 4.58 (sextet, IH), 6.80 (d, IH), 6.91 (t, IH), 6.97 (d, IH), 7.21 (t, IH), 7.26 (d, IH), 7.33 (t, IH), 8.09 - 8.05 (m, IH), 8.42 (d, IH), 8.92 (s, IH); m/z 466 (M+H)+
In a similar manner, Examples 2a-2g were prepared using the appropriate phenol and halopyridine: 4.55
(m,
(m, (t,
(m, (t,
3.63
Figure imgf000079_0001
2g 518 1HNMR O (CDCl3): 1.34 (d, 3H), 2.40
Or XY B N (M+H)+ (quintet, 2H), 2.44 (s , 3H), 3.41 (s, 3H), 3.64
- 3.49 (m, 2H), 4.45 ■ - 4.20 (m, 4H), 4.61
0 (sextet, IH), 7.04 (t, IH), 7.40 (t, IH), 7.42
(s, IH), 8.17 (d, IH), 8.21 (d, IH), 11.32 (s,
IH)
The preparation of the halopyridines is described in Example 1.
The preparation of 3 -hydroxy-5 - [( 1.S)-2-methoxy-( 1 -methylethyl)oxy] -N-( 1 -methyl- IH- pyrazol-3-yl)benzamide used in the synthesis of Examples 2 and 2a is described below:
S-Ηvdroxy-S-rd^-Σ-methoxy-d-methylethvDoxyl-N-d-methyl-lH-pyrazol-S- vDbenzamide
Figure imgf000080_0001
To a solution of 3-[(l.S)-2-methoxy-(l-methylethyl)oxy]-N-(l-methyl-lH-pyrazol-3-yl)-5- [(phenyhnethyl)oxy]benzamide (7.07 g) in TΗF (50 mL) and methanol (50 mL) was added 10% palladium on carbon (727 mg) as a slurry in TΗF (1 mL) and methanol (1 mL). The mixture was placed under vacuum and stirred under an atmosphere of hydrogen for 70 hours. The mixture was filtered through diatomaceous earth, and the diatomaceous earth washed with methanol (2 x 100 mL), followed by evaporation in vacuo. The residues were dissolved in ethyl acetate (10 mL), treated with isohexane (40 mL), the solid filtered off and washed with isohexane (50 mL) to afford the desired compound (5.17 g) which was used without further purification.
1H ΝMR δ (d6-DMSO): 1.22 (d, 3H), 3.28 (s, 3H, obscured by water), 3.38-3.53 (m, 2H), 3.76 (s, 3H), 4.65 (m, IH), 6.44 (m, IH), 6.54 (m, IH), 6.93 (s, IH), 7.04 (s, IH), 7.57 (m, IH), 9.63 (br s, IH), 10.60 (s, IH). m/z 306 (M+H)+, 304 (M-H)- B-rα^-l-Methoxy-fl-methylethvnoxyl-N-α-methyl-lH-pyrazol-S-ylVS- [(phenylmethypoxyibenzamide
Figure imgf000081_0001
A solution of 3-[(15)-2-methoxy-(l-methylethyl)oxy]-5-{[phenylmethyl]oxy}benzoic acid (8.73 g) in DCM (150 mL) was cooled to O0C. Oxalyl chloride (4.81 mL) and DMF (0.15 niL) were slowly added with stirring. The mixture was allowed to warm to ambient temperature and stirred for 16 hours, following which the organics were removed in vacuo, and the residues azeotroped with toluene (75 mL). The crude material was dissolved in DCM (75 mL) and slowly added to a stirred suspension of 3-amino-l-methylpyrazole (3.35 g) and DIPEA (14.4 mL) in DCM (75 mL). The mixture was stirred at ambient temperature for 18 hours, before the organics were evaporated in vacuo and the residue dissolved in ethyl acetate (150 mL). The organics were washed with IM aqueous hydrochloric acid (100 mL) and brine (50 mL), and dried (MgSO4), before evaporation in vacuo to give crude material. This was chromatographed on a 20Og Biotage Flash 75 SiO2 column (eluting with 30 to 90% ethyl acetate in isohexane), and evaporated in vacuo to afford the desired compound (7.07 g).
1R NMR δ (de-DMSO): 1.23 (d, 3Η), 3.28 (s, 3H, obscured by water), 3.40-3.52 (m, 2H), 3.77 (s, 3H), 4.70 (m, IH), 5.03 (s, 2H), 6.56 (m, IH), 6.71 (m, IH), 7.18 (s, IH), 7.24 (s, IH)3 7.32-7.47 (br m, 5H), 7.58 (m, IH), 10.73 (s, IH). m/z 396 (M+H)+.
3-[(15^-2-MeAoXy-(I -methylethvDoxyl-5-l [phenylmethylioxylbenzoic acid
Figure imgf000081_0002
A solution of methyl 3-[(15)-2-methoxy-(l-methylethyl)oxy]-5-{[phenylmethyl]oxy} benzoate (77.4 mmol) in a mixture of THF (232 mL) and methanol (232 mL) was treated with a solution of 2M sodium hydroxide (232 mmol), and the reaction mixture stirred for 4 hours at ambient temperature. The resulting solution was diluted with water (250 mL) and most of the organic solvent removed in vacuo. The resulting suspension was washed with diethyl ether (3 x 200 mL) and the organic washings discarded. The resulting aqueous solution was acidified to pH4 with 2M hydrochloric acid solution and extracted with ethyl acetate (2 x 200 mL). The extracts were combined, washed with brine, dried (MgSO4), and evaporated to give the desired compound (99% yield).
1H NMR δ (d6-DMSO): 1.20 (d, 3H), 3.46 (m, 2H), 4.64 (m, IH), 5.15 (s, 2H), 6.83 (app t, IH), 7.06 (s, IH), 7.13 (s, IH), 7.30-7.49 (m, 5H), 12.67 (br s, IH)
Methyl 3-FCl .SV∑-methoxy-f 1 -methylethvDoxyl -5- { [phenylmethyll oxy jbenzoate
Figure imgf000082_0001
To a solution of methyl 3-hydroxy-5-{[phenylmethyl]oxy}benzoate (77.4 mmol) in THF was added polymer-supported triphenylphosphine (51.7g of 3 mmol/g loading, 155 mmol) and (i?)-(-)-l-methoxy-2-propanol (102 mmol). The stirred solution was blanketed with argon and cooled in an ice bath. A solution of DIAD (116 mmol) was added dropwise by syringe over 10 minutes. The solution was stirred for 20 minutes and filtered, washing the residue with THF (500 mL). The filtrate and washings were combined, and evaporated to give the desired compound which was used without further purification. 1H NMR δ (d6-DMSO): 3.26 (s, 3H), 3.44 (m, 2H), 3.82 (s, 3H), 4.63 (m, IH), 5.14 (s, 2H), 6.85 (s, IH), 7.05 (s, IH), 7.11 (s, IH), 7.30-7.47 (m, 5H) The 1H NMR spectrum also contained signals consistent with a small amount of bis(l- methylethyl)hydrazine- 1 ,2-dicarboxylate.
The preparation of methyl 3-hydroxy-5-{[phenyhnethyl]oxy}benzoate is described in Example Ic.
The preparation of 3-hydroxy-5-[(l-methylethyl)oxy]-N-l,3-thiazol-2-ylbenzamide used in the preparation of Examples 2b and 2c is described below: 3-Hydroxy-5-|Y 1 -methylethyDoxy'|-N- 1.3-thiazol-2-ylbenzamide
Figure imgf000083_0001
3-[(l-Methylethyl)oxy]-5-[(phenylmethyl)oxy]-N-l,3-thiazol-2-ylbenzamide (11.2 g) was dissolved in trifluoroacetic acid (60 niL) and treated with thioanisole (17.8 mL). The mixture was left to stir at ambient temperature for 18 hours before the trifluoroacetic acid was removed in vacuo. The residues were treated with isohexane (100 mL) and the solid filtered off, before being washed with further isohexane (2 x 20 mL). The solid was dissolved in ethyl acetate (200 mL) and washed with aqueous saturated sodium hydrogen carbonate solution (100 mL). The organics were washed with water (100 mL) and brine (100 mL), and dried (MgSO4) before evaporation in vacuo to afford a solid which was washed with isohexane (200 mL) and dried in vacuo to give the desired compound (7.18 g). 1H ΝMR δ (d6-DMSO): 1.27 (d, 6H), 4.55 (m, IH), 6.49 (m, IH), 7.02 (s, IH), 7.14 (s, IH), 7.25 (d, IH), 7.54 (d, IH), 9.73 (s, IH), 12.44 (s, IH). m/z 279 (M+H)+, 277 (M-H)"
S-Cd-Methylethylioxyl-S-fCphenylmethynoxyl-N-LS-thiazol^-ylbenzamide
Figure imgf000083_0002
To a solution of 3-[(l-methylethyl)oxy]-5-[(phenylmethyl)oxy]benzoic acid (20 g) in DCM (400 mL), cooled to O0C was slowly added oxalyl chloride (12.2 mL) and DMF (0.4 mL), with stirring. The mixture was allowed to warm to ambient temperature and stirred for a further 16 hours, following which the organics were removed in vacuo, and the residues azeotroped with toluene (100 mL). The crude material was dissolved in DCM (200 mL) and slowly added to a stirred suspension of 2-aminothiazole (10.5 g) and diisopropylethylamine (24.3 mL), in DCM (200 mL). The mixture was stirred at ambient temperature for 70 hours, before the organics were removed in vacuo. The residues were dissolved in ethyl acetate (300 mL) and washed with IM aqueous hydrochloric acid (300 mL). The aqueous layer was extracted with further ethyl acetate (300 mL), and the combined organics washed with brine (75 mL), and dried (MgSO4), before evaporation in vacuo to give the desired compound (28 g) which was used without further purification.
1H NMR δ (de-DMSO): 1.27 (d, 6H), 4.70 (m, IH), 5.15 (s, 2H), 6.77 (m, IH), 7.27 (m,
2H), 7.33-7.47 (brm, 6H), 7.55 (d, IH). m/z 369 (M+H)+, 367 (M-H)";
The 1H NMR spectrum also contained signals consistent with a small amount of ethyl acetate.
3 - IY 1 -Methylethyl)oxyl-5 - lYphenylmethypoxylbenzoic acid
Figure imgf000084_0001
To a solution of methyl 3-[(l-methylethyl)oxy]-5-[(phenylmethyl)oxy]benzoate (37 g) in a
1:1 mixture of THF:methanol (300 mL) was added 4M sodium hydroxide solution (150 mL). The mixture was refluxed for 45 minutes, following which the organics were removed in vacuo. The aqueous was acidified to pH4 with hydrochloric acid (2M), and extracted with ethyl acetate. The organics were combined, washed with water and brine, dried (MgSO4) and concentrated in vacuo to give the desired compound (33.5 g), which was used without further purification.
1H NMR δ (dβ-DMSO): 1.26 (d, 6H), 4.59-4.69 (m, IH), 5.15 (s, 2H), 6.80 (app t, IH), 7.04 (m, IH), 7.12 (m, IH), 7.33 (app t, IH), 7.40 (t, 2H), 7.46 (d, 2H), 12.95 (s, IH)
Methyl 3 - IY 1 -methylethvDoxyl -5 - Ffphenylmethyrioxylbenzoate
Figure imgf000084_0002
To a solution of methyl 3-hydroxy-5-[(l-methylethyl)oxy]benzoate (25 g) in DMF (250 mL) was added anhydrous potassium carbonate (297 mmol), and benzyl bromide (143 mmol). The mixture was stirred at 6O0C for 5 hours, then cooled to room temperature. The solvent was removed in vacuo and the residue partitioned between ethyl acetate and water. The organics were combined and washed with further water, brine, dried (MgSO4) and concentrated in vacuo to give the desired compound (37 g) which was used without further purification.
1H NMR δ (d6-DMSO): 1.26 (d, 6H)5 3.84 (s, 3H), 4.61-4.70 (m, IH), 5.12 (s, 2H), 6.84 (t, IH), 7.05 (app t, IH), 7.12-7.15 (m, IH), 7.31-7.37 (m, IH), 7.40 (t, 2H), 7.46 (d, 2H)
Methyl 3 -hydroxy-5 - |Y 1 -methylethvDoxy'lbenzoate
Figure imgf000085_0001
To a stirred solution of methyl 3,5-dihydroxybenzoate (0.1 mol) in DMF (180 mL) was added powdered potassium carbonate (0.2 mol) and 2-iodopropane (0.1 mol), and the resulting mixture stirred at ambient temperature for 16 hours. The reaction mixture was poured into water (1000 mL) and the mixture extracted with ether. The extracts were combined and washed sequentially with water (twice) and brine; the solution was dried (MgSO4), filtered and evaporated in vacuo to give the crude product as a pale yellow oil (12.6 g). This was treated with toluene (40 mL) and allowed to stand overnight. The insoluble material (starting phenol) was removed by filtration, and the filtrate evaporated in vacuo. The resulting oil was chromatographed (2 x 90 g Biotage silica cartridges), eluting with hexane containing ethyl acetate (10% increasing to 15% v/v). The title compound was obtained as an oil (25% yield), which was identical by tic to a sample prepared by a similar procedure. 1H NMR δ (d6-DMSO): 1.2 (d, 6H), 3.8 (s, 3H), 4.5 - 4.6 (hept, IH), 6.55 (m, IH), 7.85 (m, IH), 7.95 (m, IH), 9.8 (s, IH)
The preparation of 3-hydroxy-5-[(l-methylethyl)oxy]-N-(l-methyl-lH-pyrazol-3- yl)benzamide used in Example 2d and 2e is described below: 3-Hvdroxy-5-|Y 1 -methylethyDoxyl-N-f 1 -methyl- lH-pyrazol-3-yl)benzamide
Figure imgf000086_0001
3-[(l-Methylethyl)oxy]-iV-(l-methyl-lH-pyrazol-3-yl)-5-[(phenylmethyl)oxy]benzamide (5 Ig; 0.14mol) was dissolved in methanol (500 mL) and TΗF (500 mL) and the flask evacuated and purged with argon (3 times). 10% Palladium on carbon (5.1 g) was added and the flask further evacuated and finally purged with hydrogen gas. The reaction mixture was stirred at ambient temperature for 20 hours. The reaction mixture was evacuated and purged with nitrogen (3 times). The catalyst was filtered off through celite, and the filtrate concentrated in vacuo. Ethyl acetate was added and filtered to give the desired compound. (30.5 g). A second crop of material was obtained in the same way (4.0 g).
1H ΝMR δ (d6-DMSO): 1.30 (d, 6H), 3.78 (s, 3H), 4.68 (sept, IH), 6.47 (m, IH), 6.60 (s, IH), 6.94 (s, IH), 7.05 (s, IH), 7.60 (s, IH), 10.63 (s, IH). m/z 276 (M+H)+
3-rd-Methylethyl)oxy1-iV-(l-methyl-lH-pyrazol-3-yl)-5-r(phenylmethyl)oxylbenzamide
Figure imgf000086_0002
DMF (2 drops) was added to a solution of 3-[(l-methylethyl)oxy]-5-
[(phenylmethyl)oxy]benzoic acid (40.0 g, 0.14 mol) and oxalyl chloride (14.6 mL, 0.17 mol) in DCM (700 mL) The mixture was stirred at ambient temperature for 4 hours and the DCM and excess oxalyl chloride evaporated in vacuo. The residual acid chloride was dissolved in DCM (300 mL) and added dropwise to l-methyl-3-aminopyrazole (14.25 g, 0.147 mol) and triethylamine (41 mL, 0.29 mol) in DCM (300 mL), at O0C. Stirred at ambient temperature for 24 hours. The DCM was evaporated in vacuo, and the residue partitioned between ethyl acetate (400 mL) and IN hydrochloric acid (200 mL). The ethyl acetate layer was washed sequentially with saturated aqueous sodium hydrogen carbonate (200 mL) and brine (100 mL), dried (MgSO4) and evaporated in vacuo. The residue was chromatographed on silica, eluting with a gradient of 50% ethyl acetate in isohexane, to give the desired compound (51 g). 1H NMR δ (CDCl3): 1.30 (d, 6H), 3.61 (s, 3H), 4.50
(sept, IH), 5.01 (s, 2H), 6.66 (m, IH), 6.88 (m, IH), 7.00 (m, IH), 7.06 (m, IH), 7.24 (m,
IH), 7.39 (m, 5H), 9.50 (s, IH). m/z 366 (M+H)+
The preparation of 3-[(l-methylethyl)oxy]-5-[(phenylmethyl)oxy]benzoic acid was described above.
The preparation of 3-hydroxy-5-{[(15)-2-methoxy-(l-methylethyl)oxy}-iV-(3-methyl- l,2,4-thiadiazol-2-yl)benzamide used in Examples 2f and 2g is described below:
S-Hvdroxy-S-ird^-Σ-methoxy-α-methylethvDoxyl-N-O-methyl-l^Λ-thiadiazol^- vDbenzamide
Figure imgf000087_0001
A solution of 3-{[(lS)-2-methoxy-(l-methylethyl)oxy}-5-{phenylmethyloxy}-N-(3- methyl- l,2,4-thiadiazol-2-yl)benzamide (9.53 g) and thioanisole (13.9 mL) in trifluoroacetic acid (45 mL) was stirred at ambient temperature for 16 hours. The trifluoroacetic acid was removed in vacuo and the residual oil partitioned between ethyl acetate (100 mL) and aqueous sodium hydrogen carbonate solution (300 mL). The aqueous layer was separated, extracted with ethyl acetate (2 x 100 mL), and the combined organic extracts washed with brine, dried (MgSO4), and evaporated to a residue which was chromatographed on silica with 50% ethyl acetate in isohexane as eluant to give the desired compound (4.5 g).
1R ΝMR δ (CDCl3): 1.2 (d, 3H), 2.5 (s, 3H), 3.3 (s, 3H), 3.4-3.6 (m, 2H), 4.6-4.7 (m, IH), 6.6 (s, IH), 7.05 (s, IH), 7.1 (s, IH), 9.85 (s, IH), 13.2 (s, IH). m/z 324 (M+H)+ 3-(r(lSV2-Methoxy-ri-methylethvDoxy)-5-(phenylmethyloxyl-N-r3-methyl-1.2.4- thiadiazol-2-vDbenzamide
Figure imgf000088_0001
To a solution of 3-[(15)-2-methoxy-(l-methylethyl)oxy]-5-{[phenylmethyl]oxy}benzoic acid (15.8 g) in DCM (260 niL) was added oxalyl chloride (5.24 mL), followed by DMF (1 drop), and the mixture stirred at ambient temperature for 16 hours. The DCM and excess oxalyl chloride were removed in vacuo, the residual oil dissolved in DCM (50 mL) and added to a solution of 5-amino-3-methyl-l,2,4 thiadiazole (6.05 g) and triethylamine (14.6 mL) in DCM (150 mL) at 0-50C, and the mixture stirred at ambient temperature for 16 hours. The DCM and excess triethylamine were removed in vacuo, and the residual oil partitioned between ethyl acetate (250 mL) and IM hydrochloric acid (150 mL). The ethyl acetate layer was separated, washed sequentially with IM hydrochloric acid, aqueous sodium hydrogen carbonate solution, and brine, dried (MgSO4), and evaporated to a residue which was chromatographed on alumina with ethyl acetate as eluant, then on silica with 30% ethyl acetate in isohexane as eluant to give the desired compound (9.6 g).
1H ΝMR δ (CDCl3): 1.3 (d, 3H), 2.45 (s,3H), 3.4 (s, 3H), 3.5-3.6 (m, 2H), 4.55-4.6 (m, IH), 5.05 (s,2H), 6.8 (s, IH), 7.1 (m, 2H), 7.25 (m, 5H), 10.7 (s, IH). m/z 414 (M+H)+
The synthesis of 3-[(lJS)-2-methoxy-(l-methylethyl)oxy]-5-{[phenylmethyl]oxy}benzoic acid is described above.
Example 3: 3-{r6-(Azetidin-l-ylcarbonyl)pyridin-3-ylloxyl-5-frflS)-l-methyl-2- (methyloxy)ethyll oxy}-iV-(l-methyl-l.ff-pyrazol-3-yl)benzamide
Figure imgf000088_0002
A mixture of 3-hydroxy-5-[(l1S)-2-methoxy-(l-methylethyl)oxy]-N-(l-methyl-lH-pyrazol- 3-yl)benzamide (0.2 g, 0.66 mmol), 5-(azetidin-l-ylcarbonyl)-2-chloropyridine (158 mg, 0.66 mmol), cesium carbonate (640 mg, 1.97 mmol) and bromotris(triphenylphosphine)copper(I) (62 mg, 0.07 mmol) in DMA (5 mL) was stirred in a 'Biotage initiator Microwave' for 3 hours. The mixture was allowed to reach RT and pressure and was partitioned between ethyl acetate (50 mL) and water (50 mL). The ethyl acetate layer was separated, washed with water (5 x 50 mL), brine (50 mL), dried (MgSO4) and evaporated to a residue which was chromatographed on silica, eluting with a gradient of 0-5% methanol in ethyl acetate, to give the desired compound (113 mg). The compound was partially crystallised from methanol. The material was also partially crystallised from isopropyl alcohol / isohexane or ethyl acetate / isohexane. Mpt (melting onset) 132.7°C. 1H ΝMR δ (CDCl3): 1.32 (d, 3H), 2.36 (quin, 2H), 3.40 (s, 3H), 3.61 - 3.47 (m, 2H), 3.78 (s, 3H), 4.25 (t, 2H), 4.58 (sextet, IH), 4.72 (t, 2H), 6.80 (t, 2H), 7.11 (s, IH), 7.31 - 7.27 (m, 2H), 7.40 - 7.35 (m, IH), 8.12 (d, IH), 8.32 (s, IH), 8.32 (s, IH). m/z 466 (MH-H)+
The following compounds were synthesised in an analogous fashion from the appropriate phenol and bromopyridine:
Figure imgf000089_0001
3c 439 1HNMR δ (CDCl3): 1.35 (d, 6H), 2.38 (M+H)4 (quintet, 2H), 4.25 (t, 2H), 4.58 (septet, IH), 4.73 (t, 2H), 6.80 (s, IH), 6.98 (d, IH), 7.20 (d, IH), 7.25 (d, IH), 7.35 (m, 2H), 8.13 (d, IH), 8.31 (d, IH), 12.15 (s, IH)
3d 436 1HNMR δ (CDCl3): 1.28 (d, 6H), 2.26 (M+H)+ (quintet, 2H), 3.69 (s, 3H), 4.18 (t, 2H), 4.49 (septet, IH), 4.63 (t, 2H), 6.65 (s, IH), 6.72
Figure imgf000090_0001
(s, IH), 7.00 (s, IH), 7.15 (m, IH), 7.20 (m, 2H), 7.28 (m, IH), 8.02 (d, IH), 8.21 (d, IH), 8.63 (s, IH)
3e 469 1HNMR δ (CDCl3): 1.33 (d, 6H), 2.36 (M+H)+ (quintet, 2H), 3.38 (s, 3H), 3.48-3.60 (m, 2H), 4.25 (t, 2H), 4.59 (sextet, IH), 4.72 (t,
Figure imgf000090_0002
2H), 6.88 (m, IH), 6.97 (d, IH), 7.19 (d, IH), 7.28 (m, 2H), 7.38 (m, 2H), 8.12 (d, IH), 8.30 (d, IH), 12.30 (s, IH)
3f 484 1HNMR δ (CDCl3): 1.27 (d, 3H), 2.29 (M+H)+ (quintet, 2H), 2.44 (s, 3H), 3.31 (s, 3H), 3.4 - 3.52 (m, 2H), 4.15 (m, 2H), 4.55 (sextet, IH),
Figure imgf000090_0003
4.65 (t, IH), 6.80 (m, IH), 7.25 (m, 2H), 7.40 (m, IH), 7.60 (m, IH), 8.00 (d, IH), 8.21 (s, IH).
3g 454 1HNMR δ (CDCl3): 1.30 (d, 6H), 2.29 (M+H)4 (quintet, 2H), 2.44 (s, 3H), 4.18 (m, 2H), c 4.55 (septet, IH), 4.65 (t, IH), 6.74 (m, IH),
Figure imgf000090_0004
7.18 (m, IH), 7.30 (m, 1 H), 7.40 (m, IH), 8.00 (d, IH), 8.23 (s, IH), 10.05 (s, IH)
The preparation of the required bromopyridines is described earlier. The preparation of 3-hydroxy-5-[(15)-2-methoxy-(l-methylethyl)oxy]-N-(l-methyl-lH- pyrazol-3 -yl)benzamide, 3 -hydroxy-5- [( 1 -methylethyl)oxy] -N-(I -methyl- lH-pyrazol-3 - yl)benzamide, 3 -hydroxy-5 - [( 1 -methylethyl)oxy] -N- 1 ,3-thiazol-2-ylbenzamide, 3 - hydroxy-5-{ [(15)-2-methoxy-(l -methylethyl)oxy} -N- 1 ,3-thiazol-2-ylbenzamide and 3- hydroxy-5-{[(l,S)-2-methoxy-(l-methylethyl)oxy}-N-(3-methyl-l,2,4-thiadiazol-2- yl)benzamide is described earlier.
The preparation of 3-hydroxy-5-[(l -methylethyl)oxy]-iV-(3 -methyl- 1 ,2,4-thiadiazol-5- yl)benzamide used in Example 3g is described below:
S-Hydroxy-S-rri-methylethvDoxyl-N-π-methyl-l^Λ-thiadiazol-S-vDbenzamide
Figure imgf000091_0001
A solution of 3-[(l -methylethyl)oxy]-N-(3-methyl- 1 ,2,4-thiadiazol-5-yl)-5- [(phenylmethyl)oxy]benzamide (33 g, 86 mmol), trifluoroacetic acid (160 mL) and thioanisole (50.5 mL) was stirred at ambient temperature for 48 hours. The TFA was removed in vacuo and the residue poured into saturated sodium bicarbonate solution (300 mL) and extracted into ethyl acetate (twice). The combined organic extracts were washed with brine, dried (MgSO4), filtered and the solvent removed in vacuo. The residue was triturated with DCM and washed with 5% ethyl acetate in isohexane to give the desired compound (12.8 g). 1H ΝMR δ (d6-DMSO): 1.31 (d, 6H), 2.51 (s, 3H), 4.67 (sept, IH), 6.58 (s, IH), 7.08 (s, IH), 7.24 (s, IH), 9.88 (s, IH), 13.25 (bra, IH). m/z294 (M+H)+
3-r(l-Methylethyl)oxy1--V-(3-methyl-1.2.4-thiadiazol-5-ylV5- [φhenylmethyl)oxy]benzamide
Figure imgf000091_0002
DMF (2 drops) was added to a solution of 3-[(l-methylethyl)oxy]-5-
[(phenyhnethyl)oxy]benzoic acid (29.6 g, 0.103 mol) and oxalyl chloride (10.78 mL, 0.12 mol) in DCM (500 mL) The mixture was stirred at ambient temperature for 4 hours and the DCM and excess oxalyl chloride removed in vacuo. The residual acid chloride was dissolved in DCM (220 mL) and added dropwise to 5-amino-3-methyl-l,2,4-thiadiazole (12.43 g, 0.108 mol) and triethylamine (30.34 niL, 0.216 mol) in DCM (220 mL), at O0C. The reaction was allowed to warm and stirred at ambient temperature for 72 hours. The DCM was removed in vacuo, and the residue partitioned between ethyl acetate (400 mL) and IN hydrochloric acid (200 mL). The ethyl acetate layer was washed sequentially with saturated aqueous sodium hydrogen carbonate (200 mL) and brine (100 mL), dried
(MgSO4) and concentrated in vacuo. The residue was chromatographed on silica, eluting with a gradient of 20% ethyl acetate in isohexane, to give the desired compound (33 g). 1H NMR δ (CDCl3): 1.32 (d, 6H), 2.31 (s, 3H), 4.51 (sept, IH), 5.05 (s, 2H), 6.74 (m, IH), 7.03 (m, IH), 7.10 (m, IH), 7.38 (m, 5H), 11.48 (brs, IH). m/z 384 (M+H)+
The preparation of 3-[(l-methylethyl)oxy]-5-[(phenylmethyl)oxy]benzoic acid is described above.
Example 4: 3-{r5-(Azetidin-l-ylsttlfonyl)pyridin-2-ylloxy}-5-(r(15)-l-methyl-2- (methyloxy)ethvnoxy}-iV-(l-methyl-lH-pyrazol-3-yl)benzamide
Figure imgf000092_0001
Triethylamine (0.025 mL, 0.18 mmol) then triethylsilane (0.96 mL, 6.03 mmol) were added to palladium (II) acetate (12 mg) in DCM (2 mL) and stirred under argon for 15 mins. 3-{ [5-(Azetidin- 1 -ylsulfonyl)-3-bromopyridin-2-yl]oxy}-5-{ [(IS)- 1 -methyl-2- (methyloxy)ethyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide (0.175 g, 0.3 mmol) in DCM (2 mL) was added dropwise and the reaction stirred at RT for 24 hours. Methanol was added, the reaction filtered through celite and the solvent removed in vacuo. Ethyl acetate (30 mL) was added and the mixture washed with water (30 mL), citric acid (30 mL), brine (30 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give a white solid. This was chromatographed on silica, eluting with a gradient of 0-100% ethyl acetate in isohexane, to give the desired compound (69 mg). 1H NMR δ (CDCl3): 1.26 (d, 3H), 2.09 (quin, 2H)5 3.33 (s, 3H), 3.41 - 3.55 (m, 2H), 3.69 (s, 3H), 3.76 (t, 4H), 4.52 (sextet, IH), 6.73 (d, IH), 6.87 (t, IH), 7.02 (d, IH), 7.17 (t, IH), 7.20 (m, IH), 7.28 (s, IH), 8.04 (m, IH), 8.54 (d, IH), 8.65 (s, IH). m/z 502 (M+H)+
The preparation of 3-{[5-(azetidin-l-ylsulfonyl)-3-bromopyridin-2-yl]oxy}-5-{[(15)-l- methyl-2-(methyloxy)ethyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide is described below:
S-irS-CAzetidin-l-ylsulfonylVS-bromopyridin^-ylloxyl-S-irα^-l-methyl-Σ-
Figure imgf000093_0001
A mixture of 3-hydroxy-5-[(15)-2-methoxy-(l-methylethyl)oxy]-N-(l-methyl-lH-pyrazol- 3-yl)benzamide (0.2g, 0.66 mmol), 5-(azetidin-l-ylsulfonyl)-3-bromo-2-chloropyridine (204 mg, 0.66 mmol) and potassium carbonate (181 mg, 1.31 mmol) in acetonitrile (5 mL) was stirred in a 'Biotage initiator Microwave' at 160°C for 2 hours. The solvent was removed in vacuo and the residue dissolved in ethyl acetate and water, the organic layer washed with brine (50 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give a brown oil which was chromatographed on silica, eluting with a gradient of 50-100% ethyl acetate in isohexane, to give the desired compound (175 mg). 1H ΝMR δ (CDCl3): 1.26 (d, 3H), 2.12 (quin, 2H), 3.33 (s, 3H), 3.54 - 3.40 (m, 2H), 3.68 (s, 3H), 3.79 (t, 4H), 4.52 (sextet, IH), 6.73 (d, IH), 6.89 (t, IH), 7.18 (t, IH), 7.21 (d, IH), 7.30 (t, IH), 8.27 (d, IH), 8.38 (d, IH), 8.77 (s, IH). m/z 580, 582 (M+H)+
5 -( Azetidin- 1 -ylsulfonyD-3 -bromo-2-chloropyridine
Figure imgf000093_0002
Azetidine hydrochloride (0.32 g, 3.44 mmol) was added to a solution of 3-bromo-2- chloropyridine-5-sulfonyl chloride (1 g, 3.44 mmol) in DCM (4 mL) and pyridine (10 mL) and stirred at RT for 22 hours. The solvent was removed in vacuo and ethyl acetate (30 mL) added. The organic phase was washed with IM hydrochloric acid (20 mL), water (20 mL), brine (20 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give the desired compound (0.4 g). 1H NMR δ (CDCl3): 2.18 (quin, 2H), 3.83 (t, 4H), 8.26 (s, IH), 8.66 (s, IH).
Example 5; 3-{r5-(Azetidin-l-ylcarbonvI)pyrazϊn-2-ylIoxy)-5-{f(lS)-l-methyI-2- (methyloxy)ethylloxy}-iV-(l-methvI-lH-pyrazol-3-yl)benzainide
Figure imgf000094_0001
DIPEA (0.28 mL, 1.59 mmol) was added to a suspension of 5-[(3-{[(lS)-l-methyl-2- (methyloxy)ethyl] oxy } -5- { [( 1 -methyl- lH-pyrazol-3 - yl)amino]carbonyl}phenyl)oxy]pyrazine-2-carboxylic acid (170 mg, 0.4 mmol), ΗATU (318 mg, 0.84 mmol) and azetidine hydrochloride (75 mg, 0.8 mmol) in DMF (5 mL) and the mixture stirred at RT for 72 hours. Ethyl acetate (40 mL) was added and washed with water (2 x 30 mL), brine (30 mL), dried (MgSO4), filtered and reduced in vacuo to give a yellow oil which was chromatographed on silica, eluting with 0-100% ethyl acetate in isohexane, to give the desired compound (48 mg). 1H NMR δ (CDCl3): 1.26 (d, 3H), 2.31 (quin, 2H), 3.32 (s, 3H), 3.41 - 3.55 (m, 2H), 3.71 (s, 3H), 4.19 (t, 2H), 4.52 (m, IH), 4.60 (t, 2H), 6.72 (s, IH)5 6.86 (m, IH), 7.16 (m, IH), 7.20 (m, IH), 7.28 (m, IH), 8.25 (s, IH), 8.52 (s, IH), 8.52 (s, IH). m/z 467 (M+H)+
The preparation of 5- [(3 - { [( 1 S)- 1 -methyl-2-(methyloxy)ethyl]oxy } -5- { [( 1 -methyl- IH- pyrazol-S-ytyaminoJcarbonylJphenytyoxyJpyrazine^-carboxylic acid is described below: S-rrS-irri^-l-Methyl-Σ-rmethyloxy^ethylloxyl-S-irfl-methyl-lH-pyrazol-S- yl)amino1carbonyllphenyl)oxylpyrazine-2-carboxylic acid
Figure imgf000095_0001
A mixture of 3-hydroxy-5-[(15)-2-methoxy-(l-metliylethyl)oxy]-N-(l-meth.yl-lH-pyrazol- 3-yl)benzamide (0.226 g, 0.74 mmol), methyl 5-chloropyrazine-2-carboxylate (192 mg, 1.11 mmol) and potassium carbonate (205 mg, 1.48 mmol) in acetonitrile (5 mL) was stirred in a 'Biotage initiator Microwave' at 160°C for 4 hours. The solvent was removed in vacuo and water (30 mL) added. The mixture was acidified and extracted into ethyl acetate (3 x 50 mL), the combined organics washed with brine (30 mL), dried (MgSO4), filtered and reduced in vacuo to give a brown oil which was a mixture of the acid and methyl ester. Lithium hydroxide monohydrate (78 mg, 1.85 mmol) in water (2 mL) was added to the mixture of acid and ester (326 mg, 0.74 mmol) in TΗF (4 mL) and the mixture stirred at RT for 20 hours. The TΗF was removed in vacuo and the aqueous residue washed with ethyl acetate (20 mL) to remove impurities then acidified with IM citric acid. The product was extracted into ethyl acetate (2 x 100 mL) and the combined organics washed with brine (50 mL), dried (MgSO4), filtered and reduced in vacuo to give a yellow solid (0.17 g). m/z 428 (M+Η)+
Example 6: 3-{r5-(Azetidin-l-ylcarbonvI)-1.3-thiazol-2-yl1oxy}-5-r(l- methvIethyI)oxyl-iV-(l-methyl-lH-pyrazol-3-yl)benzamide
Figure imgf000095_0002
Cesium carbonate (488 mg, 1.5 mmol) was added to a solution of 3-hydroxy-5-[(l- methylethyl)oxy]-N-(l-methyl-lH-pyrazol-3-yl)benzamide (187 mg, 0.5 mmol) and 5- (azetidin-l-ylcarbonyl)-2-chloro-l,3-thiazole (152 mg, 0.75 mmol) in acetonitrile (5 mL) and the stirred mixture heated at 160° in a Biotage Initiator Microwave for 2 hours. The mixture was cooled to RT and pressure, poured onto water (75 mL) then extracted with ethyl acetate (3 x 25 mL). The combined organic layers were washed with brine, dried (MgSO4) and evaporated to a residue which was chromatographed on silica eluting with ethyl acetate, to give the desired compound (142 mg). 1H NMR δ (CDCl3): 1.3 (d, 6H), 2.4 (m, 2H), 3.7 (s, 3H)5 4.1 - 4.4 (m, 4H), 4.5 (m, IH), 6.75 (s, 1H),6.9 (s, IH), 7.2 (s, IH), 7.25 (d, IH), 7.4 (s, IH) and 8.95 (s, IH). m/z 442 (M+H)+.
The following example was made in an analogous fashion from 5-(azetidin-l-ylcarbonyl)- 2-chloro-l,3-thiazole and 3-hydroxy-5-[(lS)-2-methoxy-(l-methylethyl)oxy]-N-(l-methyl- lH-pyrazol-3-yl)benzamide.
Figure imgf000096_0002
The preparation of 5-(azetidin-l-ylcarbonyl)-2-chloro-l,3-thiazole is described below:
5 -(Azetidin- 1 -ylcarbonyT)-2-chloro- 1 ,3 -thiazole
Figure imgf000096_0001
To a solution of 2-chlorothiazole-5-carboxylic acid (815 mg, 5 mmol) in dichloromethane (10 mL) was slowly added oxalyl chloride (0.53 mL, 6 mmol) and then N,N dimethylformamide (1 drop) with stirring. The mixture was stirred for 16 hours, following which the organics were removed in vacuo, and the residues azeotroped with toluene (100ml). The crude material was dissolved in dichloromethane (10 mL) and slowly added to a stirred suspension of azetidine hydrochloride (560 mg, 6 mmol) and triethylamine (2.5 mL, 18 mmol) in dichloromethane (25 mL). The mixture was stirred at ambient temperature for 2 hours before the organics were removed in vacuo. The residue was dissolved in ethyl acetate (50 mL) and water (25 mL), the organic layer washed with brine (25 mL), dried (MgSO4) and evaporated to a residue which was chromatographed on silica with 40% ethyl acetate in iso-hexane as eluant to give 5-(azetidin-l-ylcarbonyl)-2-chloro- 1,3-thiazoline. 1H NMR δ (CDCl3): 2.4 (m, 2H), 4.1 - 4.4 (m, 4H), 4.55 (m, IH), 7.2 (s, IH) and 7.75 (s, IH); m/z 203 (M+H)+.
The preparation of 3-hydroxy-5-[(l-methylethyl)oxy]-N-(l-methyl-lH-pyrazol-3- yl)benzamide and 3 -hydroxy-5 -[( 15)-2-methoxy-( 1 -methylethyl)oxy] -N-( 1 -methyl- IH- pyrazol-3-yl)benzamide are described earlier.
Example 7: S-lfS-fAzetidin-l-ylcarbonvDpyridin-l-vIioxyl-S-WliS^-hydroxy-l- methylethvIloxy}-iV-(l-methyl-lff-pyrazol-3-vI)benzamide
Figure imgf000097_0001
A solution of 6-[(3-[((15)-2-{[(l,l-dimethylethyl)(dimethyl)silyl]oxy}-l- methylethyl)oxy]-5-{[(l-methyl-lH-pyrazol-3-yl)amino]carbonyl}phenyl)oxy]pyridine-3- carboxylic acid (0.20 g, 0.378 mmol), azetidine hydrochloride (0.106 g, 0.114 mmol), ΗATU (0.302 g, 0.794 mmol) and DIPEA (0.39 mL, 0564 mmol) in DMF (7 mL) was stirred at RT overnight. 3.5M Hydrochloric acid (0.5 mL) was added and the solution left to stir for 20 minutes. The solution was neutralised with saturated sodium bicarbonate solution. Water (20 mL) was added and the solution extracted with ethyl acetate (50 mL). The ethyl acetate layer was washed with brine, dried (MgSO4), and evaporated to a residue which was chromatographed on silica, eluting with 50% ethyl acetate in isohexane, to give the desired product (27 mg). 1H ΝMR δ (CDCl3): 1.32 (d, 3H), 2.38 (quintet, 2H), 3.77 (m, 2H), 3.80 (s, 3H), 4.29 (d, 4H), 4.57 (sextet, IH), 6.81 (d, IH), 6.91 (t, IH), 6.99 (d, IH), 7.27 (m, 2H), 7.38 (m, IH), 8.42 (d, IH), 8.86 (s, IH). m/z 452 (M+H)+ The preparation of 6-[(3-[((15)-2-{[(l,l-dimethylethyl)(dimethyl)silyl]oxy}-l- methylethyl)oxy]-5-{[(l-methyl-lH-pyrazol-3-yl)amino]carbonyl}phenyl)oxy]pyridine-3- carboxylic acid is described below:
ό-rrS-rrri^-l-irri.l-Dimethylethviydimethvnsilylloxyl-l-methylethvnoxyl-S-irn- methyl-lH-pyrazol-S-vDaminolcarbonyllphenvDoxylpyridine-S-carboxylic acid
Figure imgf000098_0001
A solution of lithium hydroxide monohydrate (0.10 g, 4.17 mmol) in water (15 niL) was added to a solution of methyl 6-[(3-[((15)-2-{[(l,l-dimethylethyl)(dimethyl)silyl]oxy}-l- methylethyl)oxy]-5-{[(l-methyl-lH-pyrazol-3-yl)amino]carbonyl}phenyl)oxy]pyridine-3- carboxylate (0.90 g, 1.67 mmol) in TΗF (30 mL). The mixture was allowed to stir at RT overnight. The TΗF was removed in vaccuo and the resulting solution was partitioned between water (50 mL) and ethyl acetate (75 mL), then the ethyl acetate layer separated, washed with brine and dried (MgSO4). The aqueous layer was then taken to pΗ 7 by addition of IN hydrochloric acid (5.2 mL) and extracted with ethyl acetate (75 mL). The ethyl acetate layer was separated, washed with brine and dried (MgSO4). The ethyl acetate layers were combined and evaporated to give the required product (0.84 g). 1H NMR δ (CDCl3): 0.05 (d, 6H), 0.88 (s, 9H), 1.36 (d, 3H), 3.70-3.88 (m, 5H), 4.52-4.61 (sex, IH), 6.98 (d, 2H), 7.01 (d, IH), 6.28 (s, IH), 7.44 (s, IH), 7.57 (s, IH), 8.04 (m, IH), 8.72 (s, IH), 10.62 (s, IH). m/z 527 (M+H)+
Methyl ό-rG-rcα^^-irriJ-dimethylethylϊdimethvnsilylloxyl-l-methylethvnoxyl-S- { IY 1 -methyl- lH-pyrazol-3 -vDaminol carbonyl )ρhenvDoxy1pyridine-3 -carboxvlate
Figure imgf000098_0002
A solution of 3-[((15)-2-{ [(I ,l-dimethylethyl)(dimethyl)silyl]oxy}-l-methylethyl)oxy]-5- hydroxy-N-(l-methyl-lH-pyrazol-3-yl)benzamide (1.00 g, 2.47 mmol), methyl 6- chloronicotinate (0.450 g, 2.60 mmol), and cesium carbonate (1.204 g, 3.71 mmol) in acetonitrile (15 mL) was heated at 160°C using microwave heating for 90 minutes. The acetonitrile was removed in vaccuo and the residual oil partitioned between water (50 mL) and ethyl acetate (75 mL). The ethyl acetate layer was separated, washed with brine, dried (MgSO4), and evaporated to a residue which was chromatographed on silica, eluting with 50% ethyl acetate in isohexane, to give the desired compound (0.977 g). 1R ΝMR δ (CDCl3): 0.05 (d, 6Η), 0.87 (s, 9H), 1.34 (d, 3H), 3.54 (m, 3H), 3.79 (s, 3H), 3.93 (s, 3H), 4.59 (sextet, IH), 6.79 (d, IH), 6.92 (t, IH), 6.96 (s, IH), 6.99 (s, IH), 7.20 (t, IH), 7.27 (d, IH), 7.33 (t, IH), 8.28 (d, IH)5 8.31 (d, IH), 8.39 (s, IH), 8.82 (d, IH). m/z (M+H)+541
3-rr(15f)-2-{r(l.l-DimethylethviydimethvDsilylloxyl-l-methylethvnoxy1-5-hvdroxy-N-(l- methyl- lH-pyrazol-3-yr)benzamide
Figure imgf000099_0001
3-[((15)-2-{[(l,l-dimethylethyl)(dimethyl)silyl]oxy}-l-methylethyl)oxy]-N-(l-methyl-lH- pyrazol-3-yl)-5-[(phenylmethyl)oxy]benzamide (1.8 g, 3.64 mmol) was dissolved in methanol (50 mL) and the flask evacuated and purged with nitrogen (3 times). 10% Palladium on carbon (0.2 g) was added and the flask further evacuated and finally purged with hydrogen gas. The reaction mixture was stirred at ambient temperature for 16 hours until completion. The reaction mixture was evacuated and purged with nitrogen (3 times). The catalyst was filtered off, and the filtrate concentrated in vacuo to give the desired compound (1.45 g). 1H ΝMR δ (d6-DMSO): 0.02 (d, 6H), 0.83 (s, 9H)5 1.18 (d, 3H), 3.66 (m, 2H)5 3.72 (s, 3H), 4.51 (m, IH)5 6.42 (m, IH)5 6.52 (m, IH)5 6.90 (s, IH), 7.02 (s, IH)5 7.55 (m, IH), 9.58 (br s5 IH)5 10.59 (br s5 IH). m/z 406 (M+H)+ S-frd^-Σ-irriJ-Dimethylethylϊdimethvnsilylloxyμi-methylethvnoxyl-N-d-methyl- lH-pwazol-3-ylV5-r(ρhenylmethyl)oxy1benzamide
Figure imgf000100_0001
DIPEA (4.06 g5 23.4 mmol) was added to a suspension of 3-[((lS)-2-{[(l,l- dimethylethyl)(dimethyl)silyl]oxy}-l-methylethyl)oxy]-5-[(phenylmethyl)oxy]benzoic acid (2.43 g, 5.84 mmol), 3-amino-l-methylpyrazole (0.85 g, 8.76 mmol) and ΗATU (4.66 g, 12.3 mmol) in DMF (50 mL) and stirred at ambient temperature for 16 hours. The resultant mixture was partially reduced in vacuo, poured onto water (100 mL) and extracted with diethyl ether (2 x 50 mL). The extracts were washed with water and brine then dried (MgSO4), filtered and reduced to an opaque gum which partially crystallized. The crude product was purified by column chromatography, eluting with 0-100% ethyl acetate in isohexane, to give the title compound as a colourless oil (1.87g). 1H ΝMR δ (d6-DMSO): 0.02 (d, 6H), 0.84 (s, 9H), 1.21 (d, 3H), 3.68 (d, 2H), 3.76 (s, 3H), 4.58 (m, IH), 5.13 (s, 2H), 6.56 (m, IH), 6.70 (m, IH), 7.18 (s, IH), 7.24 (s, IH), 7.29- 7.46 (m, 5H), 7.57 (m, IH), 10.74 (br s, IH). m/z 496 (M+H)+
3-IT(I S)-2- { IY 1.1 -Dimethylethylϊdimethvnsilylioxyl - 1 -methylethvnoxyl-5- rfohenylmethvDoxylbenzoic acid
Figure imgf000100_0002
Methyl 3-[((l S)-I- {[(1,1 -dimethylethyl)(dimethyl)silyl]oxy } - 1 -methylethyl)oxy]-5-
[(phenylmethyl)oxy]benzoate (3.0 g, 6.98 mmol) was dissolved in THF (50 mL) and water (1OmL) and lithium hydroxide monohydrate (586 mg, 13.95 mmol) added. The resultant mixture was heated with stirring at 450C for 2 hours, then at ambient temperature for 16 hours, and at 450C for a further 4 hours. Water (40 mL) was added and the solvent removed in vacuo. The resultant solution was acidified carefully with IM citric acid (2 equivalents), washed with water and brine then dried (MgSO4), filtered and evaporated in vacuo to give the title compound as a colourless gum (2.58 g). 1HNMR δ (d6-DMSO): 0.02 (d, 6H), 0.84 (s, 9H), 1.17 (d, 3H), 3.66 (m, 2H), 4.43 (m, IH), 5.05 (s, 2H), 6.56 (br s, IH), 7.10 (br s, IH), 7.17 (br s, IH), 7.25-7.44 (m, 5H), 7.60 (br s, IH).
Methyl 3-[(q.y)-2-{[α.l-dimethylethviydimethvnsilylloxy>-l-methylethvnoxyl-5- |"(phenylmethyl)oxy]benzoate
Figure imgf000101_0001
(2i?)-l-{[(l,l-Dimethylethyl)(dimethyl)silyl]oxy}propan-2-ol (3.31 g, 17.4 mmol) was added to a solution of methyl 3-hydroxy-5-{[phenylmethyl]oxy}benzoate (3.00 g, 11.6 mmol) in THF (50 mL) at O0C followed by addition of triphenylphosphine (4.57 g, 17.4 mmol) then DIAD (3.43 mL, 17.4 mmol) and the reaction was warmed to RT and stirred for 16 h. The reaction was quenched with water (100 mL) and diethyl ether (400 mL) and the organic layer was separated then dried (MgSO4) and evaporated. Purification by column chromatography, eluting with 1:15 to 1:5 ethyl acetate:hexane, afforded the title compound as a colourless oil (4.00 g, 80%). 1H NMR δ (CDCl3): 0.03 (s, 3H), 0.05 (s, 3H), 0.89 (s, 9H), 1.29 (d, 3H), 3.63 (dd, IH), 3.78 (dd, IH), 3.92 (s, 3H), 4.44 (m, IH), 5.08 (s, 2H), 6.77 (m, IH), 7.40 (m, 7H)
The preparation of methyl 3-hydroxy-5-{[phenyhnethyl]oxy}benzoate was described earlier. (2R)- 1 -( IYl ■ 1 -Dimethylethyl¥dimethvDsilyl1oxy)propan-2-ol
7>
fert-Butyl(dimethyl)silyl chloride (5.90 g, 39.5 mmol) was added to a solution of (2R)- proρane-l,2-diol (3.00 g, 39.5 mmol) in DCM (100 mL) followed by diisopropylethylamine (7.10 g, 55.3 mmol) and the reaction was stirred under argon for 72 h. The reaction was diluted with diethyl ether (500 mL) and water (140 mL) and the organic layer was separated then dried (MgSO4), filtered and evaporated. Purification by column chromatography, eluting with 1:15 to 1:10 ethyl acetate: hexane, afforded the title compound as a colourless oil (6.00 g, 80%). 1H NMR δ (CDCl3): 0.10 (m, 6H), 0.92 (s, 9H), 1.14 (d, 3H), 2.42 (d, IH), 3.38 (dd, IH), 3.60 (dd, IH), 3.82 (m, IH). The data matched that reported in the literature (J. Org. Chem., 1998, 53_, 2300).
Example 8: S-ffS-Chloro-S-dnorpholin^-ylcarbonvDpyridin-l-vnoxyl-S-irdS)^- hvdroxy-l-methvIethylloxy>-iV-(l-methvI-lH/-pyrazoI-3-yl)benzamide
Figure imgf000102_0001
A solution 5-chloro-6-[(3-[((15)-2-{[(l,l-dimethylethyl)(dimethyl)silyl]oxy}-l- methylethyl)oxy] -5 - { [( 1 -methyl- lH-pyrazol-3 -yl)amino] carbonyl }phenyl)oxy]pyridine-3 - carboxylic acid (0.15 g, 0.267 mmol), morpholine (0.07 mL, 0.803 mmol), ΗATU (0.213 g, 0.560 mmol) and DIPEA (0.15 mL, 0.861 mmol) in DMF (7 mL) was stirred at RT for 2 days. Water (20 mL) was added and the solution extracted with ethyl acetate (50 mL). The ethyl acetate layer was separated, washed with brine and dried (MgSO4), and evaporated to a residue which was chromatographed on silica, eluting with 1% methanol in ethyl acetate. 3.5M Hydrochloric acid (0.5 mL) was added to a solution of the residual solid taken up in methanol (5 mL) and allowed to stir at RT for 20 minutes. The solution was neutralised with saturated sodium bicarbonate solution and the methanol removed in vaccuo and the residual solution partitioned between water (20 mL) and ethyl acetate. The ethyl acetate layer was separated and washed with brine, dried (MgSO4) and evaporated to give the desired product (34 mg). 1H NMR δ (d6-DMSO): 1.28 (d, 3H), 3.58 (m, 10H), 3.81 (s, 3H), 4.61 (sextet, IH), 4.89 (t, IH), 6.60 (d, IH), 7.03 (t, IH), 7.41 (s, IH), 7.54 (s, IH), 7.63 (d, IH), 8.20 (q, 2H), 10.84 (s, IH). m/z 516 (M+H)+
The preparation of 5-chloro-6-[(3-[((l1S)-2-{[(l,l-dimethylethyl)(dimethyl)silyl]oxy}-l- methylethyl)oxy]-5-{[(l-methyl-lH-pyrazol-3-yl)amino]carbonyl}phenyl)oxy]pyridine-3- carboxylic acid is described below:
5-Chloro-6-rr3-rr(15)-2-{r(l.l-dimethylethviydimethvnsilylloxyl-l-methylethvnoxyl-5- { IY 1 -methyl- lH-pyrazol-3 -vDaminol carbonvUphenvDoxyipyridine-3 -carboxylic acid
Figure imgf000103_0001
To a solution of methyl 5-chloro-6-[(3-[((15)-2-{[(l,l-dimethylethyl)(dimethyl)silyl]oxy}- 1 -methylethyl)oxy] -5- { [( 1 -methyl- lH-pyrazol-3 -yl)amino] carbonyl }phenyl)oxy]pyridine- 3-carboxylate (1.2 g, 2.09 mmol) in TΗF (50 mL) was added a solution of lithium hydroxide monohydrate (0.219 g, 5.22 mmol) in water (30 mL). The mixture was allowed to stir under RT overnight. The TΗF was removed in vaccuo and the resulting solution was partitioned between water (50 mL) and ethyl acetate (75 mL), and the ethyl acetate layer separated, washed with brine and dried (MgSO4). The aqueous layer was then adjusted to pΗ 7 by addition of IN hydrochloric acid (5.2 mL) and partitioned between ethyl acetate (75 mL). The ethyl acetate layer was separated, washed with brine and dried (MgSO4). The ethyl acetate layers were combined and evaporated to give the required product (1.1 g). 1U NMR δ (d6-DMSO): 0.01 (d, 6Η), 0.82 (s, 9H), 1.22 (d, 3H), 3.73 (m, 2H), 3.76 (s, 3H), 4.55 (sextet, IH), 6.55 (d, IH), 6.90 (t, IH), 7.30 (s, IH), 7.44 (s, IH), 7.58 (d, IH), 8.36 (d, IH), 8.19 (d, IH), 8.51 (s, IH). m/z (M+H)+ 562 Methyl 5-chloro-6-rr3-rr(l^-2-(rri.l-dimethylethviydimethvnsilylloxyl-l- methylethvDoxyl-S-ird-methyl-lH-pyrazol-S-vDaminolcarbonvUphenvDoxyipyridine-S- carboxylate
Figure imgf000104_0001
A solution of 3-[((15)-2-{[(l,l-dimethylethyl)(dimethyl)silyl]oxy}-l-methylethyl)oxy]-5- hydroxy-N-(l-methyl-lH-pyrazol-3-yl)benzamide (1.0 g, 2.47 mmol), ethyl 5,6- dichloronicotinate (0.57 g, 2.59 mmol), and potassium carbonate (0.855 g, 6.14 mmol) in acetonitrile (15 mL) was heated at 160°C for 4 hours using microwave heating. The acetonitrile was removed in vaccuo and the residual oil partitioned between water (50 mL) and ethyl acetate (75 mL). The ethyl acetate layer was separated, washed with brine, dried (MgSO4), and evaporated to a residue which was chromatographed on silica, eluting with 50% ethyl acetate in hexane to give the desired compound (1.1 g). The analytical data indicated that transesterification had occurred at some stage in the procedure to furnish the methyl ester. 1H ΝMR δ (CDCl3): 0.05 (d, 6H), 0.87 (s, 9H), 1.30 (d, 3H), 3.72 (s, 3H), 3.73 (m, IH), 3.93 (s, 3H), 4.47 (sextet, IH), 6.80 (d, IH), 6.93 (t, IH), 7.19 (t, IH), 7.25 (d, IH), 7.26 (s, IH), 7.34 (t, IH), 8.35 (d, IH), 8.61 (d, IH), 8.97 (s, IH); m/z (M+H)+575
The preparation of 3-[((15)-2-{[(l,l-dimethylethyl)(dimethyl)silyl]oxy}-l- methylethyl)oxy]-5-hydroxy-N-(l-methyl-lH-pyrazol-3-yl)benzamide is described earlier.
Example 9 : 3-{ f 5-(Azetidin-l-ylcarbonyl)-3-chloropyridin-2-yll oxy}-5-{ f 2-fluoro-l- (fluoromethyI)ethvnoxy}-iV-(l-methyl-lH-pyrazol-3-yl)benzamide
Figure imgf000104_0002
A mixture of 3-{[2-fluoro-l-(fluoromethyl)ethyl]oxy}-5-hydroxy-N-(l-methyl- IH- pyrazol-3-yl)benzamide (200 mg, 0.64 mmol), 5-(azetidin-l-ylcarbonyl)-2,3- dichloropyridine (164 mg, 0.71 mmol) and potassium carbonate (178 mg, 1.28 mmol) in acetonitrile (5 mL) was stirred in a 'Biotage initiator Microwave'at 120°C for 4 hours. The solvent was removed in vacuo and the residue partitioned between ethyl acetate and water (100 mL), the organic layer washed with brine (50 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give a brown oil which was chromatographed on silica, eluting with a gradient of 50-100% ethyl acetate in isohexane, to give the desired compound (237 mg). 1H NMR δ (CDCl3): 2.39 (quintet, 2H), 3.74 (s, 3H), 4.25 (t, 2H), 4.37 (t, 2H), 4.63 (m, 2H), 4.74 (m, 3H), 6.81 (s, IH), 7.01 (s, IH), 7.29 (m, IH), 7.31 (s, IH), 7.42 (s, IH), 8.17 (s, IH), 8.23 (s, IH), 9.26 (s, IH). m/z 506 (M+H)+
The preparation of 5-(azetidin-l-ylcarbonyl)-2,3-dichloropyridine is described above. The preparation of 3-{[2-fluoro-l-(fluoromethyl)ethyl]oxy}-5-hydroxy-N-(l-methyl-lH- pyrazol-3-yl)benzamide is described below:
3-1 [2-Fluoro- 1 -CfluoromethyDethyl] oxy) -5-hvdroxy-N-( 1 -methyl- lH-pyrazol-3 - vDbenzamide
Figure imgf000105_0001
A solution of 3-{[2-fluoro-l-(fluoromethyl)ethyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)-5- [(phenylmethyl)oxy]benzamide (2.46 g, 6.13 mmol) and 10% by weight palladium on carbon (0.246 g) in ethanol (100 mL) was allowed to stir at RT, under a hydrogen atmosphere overnight. The solution was filtered through Celite ® and the cake washed with methanol (100 mL). The solution was evaporated to give the desired compound (1.78 g). 1H NMR δ (d6-DMSO): 3.78 (s, 3H), 4.72 (m, 4H), 4.97 (m, IH), 6.57 (d, 2H), 7.03 (s, IH), 7.16 (s, IH), 7.59 (s, IH). m/z 312 (M+H)+ S-ir∑-Fluoro-l-Cfluoromethyllethylioxyl-iV-ri-methyl-lH-pyrazol-S-ylVS- [(phenylmethvDoxyibenzamide
Figure imgf000106_0001
A solution 3-{[2-fluoro-l-(fluoromethyl)ethyl]oxy}-5-[(phenylmethyl)oxy]benzoic acid (3.00 g, 9.31 mmol), 3-amino-l-methylpyrazole (1.83 g, 18.6 mmol), ΗATU (4.60 g, 12.1 mmol) and DIPEA (3.25 mL, 18.6 mmol) in DMF (12 mL) was stirred at RT overnight. Water (150 mL) was added and the solution partitioned with ethyl acetate (250 mL). The ethyl acetate layer was separated, washed with brine and dried (MgSO4), and evaporated to a residue which was chromatographed on silica, eluting with 50% ethyl acetate in isohexane, to give the desired product (2.46 g).
1H NMR δ (CDCl3): 3.69 (s, 3H), 4.57 (m, 5H), 5.00 (s, 2H), 6.70 (t, IH), 6.74 (d, IH), 7.01 (t, IH), 7.08 (t, IH), 7.21 (d, IH), 7.30 (m, 5H), 8.68 (s, IH); m/z 402 (M+H)+
3-l|"2-Fluoro-l-rfluoromethyl)ethyl]oxy}-5-[(phenylmethyl)oxylbenzoic acid
Figure imgf000106_0002
A solution of lithium hydroxide monohydrate (2.32 g, 55.1 mmol) in water (100 mL) was added to a solution of methyl 3-{[2-fluoro-l-(fluoromethyl)ethyl]oxy}-5- [(phenylmethyl)oxy]benzoate (7.41 g, 22.0 mmol) in THF (200 mL) and the mixture allowed to stir at RT overnight. The THF was removed in vacuo and the resulting solution partitioned between water (100 mL) and ethyl acetate (250 mL). The ethyl acetate layer was separated, washed with brine and dried (MgSO4). The aqueous layer was then adjusted to pH 7 by addition of IM hydrochloric acid and extracted with ethyl acetate (75 mL). The ethyl acetate layer was separated, washed with brine and dried (MgSO4). The ethyl acetate layers were combined and evaporated to give the required product (6.404 g). 1H NMR δ (d6-DMSO): 4.74 (m, 4H), 5.08 (s, 2H), 6.67 (s, IH), 6.67 (s, IH), 7.23 (s, IH), 7.37 (m, 5H). m/z 231 (M-H)"
Methyl S-ir∑-fluoro-l-CfluoromethvDethylloxyl-S-rrplienylmetlivDoxylbenzoate
Figure imgf000107_0001
DIAD (7.63 mL, 38.7 mmol) was added in a dropwise fashion to a solution of methyl 3- hydroxy-5-{[phenylmethyl]oxy}benzoate (5.00 g, 19.4 mmol), l,3-difluoropropan-2-ol (3 mL, 38.7 mmol), and triphenylphosphine (10.16 g, 38.7 mmol) in THF (100 mL) under an inert atmosphere at 0°C. The solution was allowed to reach RT and left to stir for 2 days. The THF was removed in vacuo and the residual oil slurried with a mixture of 20% ethyl acetate in isohexane. After allowing to stir for 90 minutes the mixture was filtered and the filtrate evaporated. The residual was oil chromatographed on silica, eluting with 30% ethyl acetate in isohexane, to give the desired compound (7.4Ig). 1H NMR δ (d6-DMSO): 3.85 (s, 3H), 4.71 (m, 4H), 5.03 (m, IH), 5.17 (s, 2H), 7.01 (t, IH), 7.20 (m, 2H), 7.40 (m, 5H). m/z 335 (M-H)"
The preparation of methyl 3-hydroxy-5-{[phenyhnethyl]oxy}benzoate is described above.
Example 10; B-lfS-fAzetidin-l-ylcarbonvD-S-chloropyridin-I-ylloxyl-S-l [(1S)-I- methvIpropynoxy>-JV-(l-methyI-lH-pyrazol-3-vI)benzamide
Figure imgf000107_0002
A solution of 3-hydroxy-5-{[(15)-l-methylpropyl]oxy}-N-(l-methyl-lH-pyrazol-3- yl)benzamide (116 mg, 0.4 mmol) and the 5-(azetidin-l-ylcarbonyl)-2,3-dichloropyridine (111 mg, 0.48 mmol) in acetonitrile (2 mL) containing potassium carbonate (111 mg, 0.8 mmol) was heated with stirring in the microwave reactor for 6 hours at 16O0C. The reaction mixture was filtered and the filtrate evaporated to dryness under reduced pressure and purified by chromatography on silica, eluting with 50-100% ethyl acetate in hexane, to give the required product (177 mg). 1H NMR δ (CDCl3): 0.91 (t, 3H), 1.25 (d, 3H), 1.52 - 1.75 (m, 2H), 2.32 (quin, 2H), 3.72 (s, 3H), 4.17 (t, 2H), 4.25 - 4.34 (m, 3H), 6.73 (d, IH), 6.81 (t, IH), 7.12 (s, IH), 7.21 (d, IH), 7.24 (s, IH), 8.09 (d, IH), 8.18 (d, IH), 8.54 (s, IH); m/z 484 (M+H)+
The preparation of 5-(azetidin-l-ylcarbonyl)-2,3-dichloropyridine was described earlier. The preparartion of 3-hydroxy-5-{[(liS)-l-methylpropyl]oxy}-N-(l-methyl-lH-pyrazol-3- yl)benzamide is described below:
S-Ηydroxy-S-irdiS^-l-methylpropylioxyl-N-d-meihyl-lH-pyrazol-S-yDbenzamide
Figure imgf000108_0001
A solution of 3- { [(1S)- l-methylpropyl]oxy} -N-(I -methyl- lH-pyrazol-3-yl)-5-
[(phenylmethyl)oxy]benzamide (900 mg) in TΗF (5 mL) and ethanol (5 mL) containing 10% palladium on carbon was stirred under an atmosphere of hydrogen overnight. The palladium on carbon was removed by filtration and the filtrate evaporated under reduced pressure to give the required compound as a white solid (683 mg). 1H ΝMR δ (CDCl3): 0.95 (t, 3H), 1.27 (d, 3H), 1.54 - 1.80 (m, 2H), 3.79 (s, 3H), 4.31 (q, IH), 6.57 (t, IH), 6.81 (d, IH), 6.96 (s, IH), 6.98 (s, IH), 7.30 (s, IH), 7.57 (s, IH), 8.84 (s, IH); m/z 290(M+H)+
3-(rd,S)-l-Methvbropynoxyl-N-ri-methyl-lH-pyrazol-3-ylV5- rrphenylmethylioxylbenzamide
Figure imgf000108_0002
HATU (1.19 g, 3.13 mmol) was added to 3-{[(lS)-l-methylρropyl]oxy}-5- [(phenylmethyl)oxy]benzoic acid (750 mg, 2.5 mmol) and 3-amino-l methyl pyrazole (291 mg, 3 mmol) in DMF (5 mL) followed by addition of DIPEA (0.807 mL, 6.25 mmol) and the reaction was stirred for 16 hours. The reaction mixture was added to ethyl acetate (30 mL), washed with water (10 mL), 2N citric acid (10 mL), saturated aqueous sodium hydrogencarbonate (10 mL) and brine (10 mL), then dried (MgSO4), filtered and evaporated to a gummy residue. The residue was taken up in 50% ether in ethylacetate (50 mL), washed with 2N hydrochloric acid (10 mL), water (10 mL), saturated aqueous sodium hydrogencarbonate (10 mL) and brine (10 mL) then dried (MgSO4), filtered and evaporated to a foam (900 mg ) which was used without further purification in the next step. 1H NMR o (CDCl3): 0.96 (t, 3H), 1.28 (d, 3H), 1.51-1.80 (m, 2H), 3.78 (s, IH), 4.31 (q, IH), 5.06 (s, 2H), 6.68 (t, IH), 6.82 (d, IH), 7.00 (s, IH), 7.06 (s, IH), 7.29 (s, 3H), 7.31 - 7.47 (m, 5H), 8.63 (s, IH); m/z 380 (M+H)+
S-lfdS^-l-Methylpropylloxyl-S-ITphenylmethvDoxylbenzoic acid
Figure imgf000109_0001
Methyl 3-{[(15)-l-methylpropyl]oxy}-5-[(phenylmethyl)oxy]benzoate (14.87 g, 47.36 mmol) was vigorously stirred in a mixture of aqueous IM sodium hydroxide (120 mL) and THF at approximately 450C for 4 hours. The bulk of the THF was removed under reduced pressure and the resultant solution partitioned between water and diethyl ether. The aqueous phase was acidified with 2M hydrochloric acid and then extracted with ethyl acetate. The organics were dried (MgSO4) and concentrated in vacuo gave a white solid (11.5 g). 1R NMR δ (CDCl3): 0.90 (t, 3H), 1.20 (d, 3H), 1.49 - 1.69 (m, 2H), 4.33 - 4.46 (m, IH), 5.12 (s, 2H), 6.75 - 6.79 (m, IH), 6.99 - 7.03 (m, IH), 7.06 - 7.11 (m, IH), 7.26 - 7.47 (m, 5H) Methyl 3-(1YIiSVl -methylpropyl]oxy } -5- [rphenylmethvDoxylbenzoate
Figure imgf000110_0001
A solution of DIAD (16.6 mL, 84.0 mmol) in dry THF (100 mL) was added dropwise to a cooled (ice bath) stirred mixture of methyl 3-hydroxy-5-{[phenylmethyl]oxy}benzoate (14.5 g, 56.2 mmol); R-(-)-sec-butanol (5 g, 67.0 mmol) and solid-supported triphenylphosphine (28 g, of 3 mmol per g loading, 84.0 mmol) in dry THF (400 mL) whilst maintaining the temperature below 10°C. The mixture was allowed to stir for 3 hours, then diluted with diethyl ether (800 mL) and filtered. Removal of the solvent gave a pale coloured oil, which was purified by column chromatography on silica, eluting with 25% ethyl acetate in hexane; to give the desired product (14.87 g).
1H NMR 6 (CDCl3): 0.99 (t, 3H), 1.28 (d, 3H), 1.53 - 1.80 (m, 2H), 3.89 (s, 3H), 4.25 - 4.42 (m, IH), 5.07 (s, 2H), 6.68 - 6.81 (m, IH), 7.17 - 7.53 (m, 7H); m/z 313 (M-H)"
The preparation of methyl 3-hydroxy-5-{[phenylmethyl]oxy}benzoate was described earlier.
Example 11 : 3-{ [5-(Azetidin-l-ylcarbonvI)pyrazin-2-yIloxyl-5-U(lS)-l- methylpropyIloxy}-iV-(l-methyl-liy-pyrazoI-3-yl)benzamide
Figure imgf000110_0002
HATU (34 mg, 0.89 mmol) was added to 5-[(3-{[(15)-l-methylpropyl]oxy}-5-{[(l- methyl-lH-pyrazol-S-y^aminojcarbonylJpheny^oxyjpyrazine^-carboxylic acid (190 mg, 0.4 mmol) and azetidine hydrochloride (84 mg, 0.89 mmol) in DMF (5 mL). DIPEA (0.311 mL, 1.79 mmol) was added and the reaction was stirred for 16 hours. The reaction mixture was added to ethyl acetate (20 mL), washed with water (10 mL), 2N citric acid (10 mL), saturated aqueous sodium hydrogencarbonate (10 mL) and brine (10 mL), then dried (MgSO4), filtered and evaporated under reduced pressure to a gummy residue. The residue was purified by chromatography on silica, eluting with 50-100% ethyl acetate in hexane, to give a gum which was taken up in 50% ethyl acetate in ether and washed twice with water, brine (10 mL) then dried (MgSO4) to give the desired product as a foam (58 mg).
1H NMR O (CDCl3): 0.91 (t, 3H), 1.25 (d, 3H), 1.51 - 1.75 (m, 2H), 2.31 (quin, 2H), 3.72 (s, 3H), 4.19 (t, 2H), 4.29 (q, IH), 4.62 (t, 2H), 6.73 (d, IH), 6.80 (t, IH), 7.13 (t, IH), 7.21 (d, IH), 7.23 (t, IH), 8.26 (s, IH), 8.52 (s, IH), 8.79 (s, IH); m/z 451 (M+H)+
The preparation of 5-[(3-{[(15)-l-methylpropyl]oxy}-5-{[(l-methyl-lH-pyrazol-3- yl)amino]carbonyl}phenyl)oxy]pyrazine-2-carboxylic acid is described below:
5-r(3-irfl5)-l-Me<hylpropylloxy)-54r(l-methyl-l.g-pyrazol-3- yl")amino1carbonyl|phenyl)oxylpyrazine-2-carboxylic acid
Figure imgf000111_0001
A solution of 3-hydroxy-5-{[(liS)-l-methylpropyl]oxy}-N-(l-methyl-lH-pyrazol-3- yl)benzamide (116 mg, 0.4 mmol) and the methyl 5-chloropyrazine-2-carboxylate (83 mg, 0.48 mmol) in acetonitrile (2 mL) containing potassium carbonate (111 mg, 0.8 mmol) was heated with stirring in the microwave for 6 hours at 16O0C. Analytical LC-MS showed formation of the acid but no ester. The reaction mixture was dissolved in water (10 mL), acidified with 2Ν citric acid and extracted with ethyl acetate (5 x 20 mL), washed with brine, dried (MgSO4) and evaporated to dryness under reduced pressure to deliver the desired product (190 mg) which was used without purification in the next step. w/z 412 (M+Η)+
The preparation of 3-hydroxy-5- { [( 1 S)- 1 -methylpropyl] oxy } -N-( 1 -methyl- lH-pyrazol-3 - yl)benzamide was described earlier. Example l2: 3-{f6-(azetidin-l-vIcarbonvI)pyridin-3-ylloxy}-5-(rflS)-l- methyIpropvnoxv>-N-(l-methvI-lf?-Pvrazol-3-vπbenzamide
Figure imgf000112_0001
A mixture of of 3-hydroxy-5-{[(15)-l-methylpropyl]oxy}-N-(l-methyl-lH-pyrazol-3- yl)benzamide (0.116 g, 0.4 mmol), 2-(azetidin- 1 -ylcarbonyl)-5-bromopyridine (116 mg, 0.48 mmol), cesium carbonate (392 mg, 1.2 mmol) and bromotris(triphenylphosphine)copper(I) (38 mg, 0.04 mmol) in DMA (5 mL) was stirred in the microwave reactor at 16O0C for 4 hours. The product was dissolved in ethyl acetate (20 mL) and water (15 mL) and the layers separated. The organic layer was washed with brine (50 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give a brown oil which was chromatographed on silica, eluting with a gradient of 0-5% methanol in ethyl acetate, and then re-columned on silica, eluting with a gradient of 0-5% methanol in DCM, to give the desired compound as a white foam which crystallised on standing. The material was triturated with 50% ethyl acetate in hexane to give the required product as a white solid (95 mg). 1H ΝMR δ (CDCl3): 0.90 (t, 3H), 1.23 (d, 3H), 1.51 - 1.72 (m, 2H), 2.28 (quin, 2H), 3.73 (s, 3H), 4.18 (t, 2H), 4.28 (q, IH), 4.64 (t, 2H), 6.67 (t, IH), 6.72 (d, IH), 6.99 (s, IH), 7.15 (s, IH), 7.22 (d, IH), 7.31 (d, IH), 8.04 (d, IH), 8.26 (s, IH), 8.41 (s, IH); m/z 450 (M+H)+
The preparation of 3-hydroxy-5-{[(15)-l-methylpropyl]oxy}-N-(l-methyl-lH-pyrazol-3- yl)benzamide and 2-(azetidin-l-ylcarbonyl)-5-bromopyridine were described earlier. Example 13: 3-U2-(Azetidin-l-ylcarbonyr)pyrimidin-5-ylloxy}-5-[(l- methylethyI)oxyl-iV-(l-methyl-llZ-pyrazol-3-yl)benzamide
Figure imgf000113_0001
A mixture of 3-hydroxy-5-[(l-methylethyl)oxy]-N-(l-methyl-lH-pyrazol-3-yl)benzamide (250 mg, 0.91 mmol), 2-(azetidin-l-ylcarbonyl)-5-bromopyrimidine (242 mg, 1.0 mmol), cesium carbonate (886 mg, 2.72 mmol) andbromotris(triphenylphosphine)copper(I) (423 mg, 0.45 mmol) in DMA (5 mL) was stirred in a 'Biotage initiator Microwave' at 16O0C for 4 hours. The mixture was added to ethyl acetate (50 mL) and water (50 mL), the organic layer washed with brine (50 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give a brown oil. The residue was chromatographed on silica, eluting with a gradient of 0-10% methanol in ethyl acetate, to give the desired compound (199 mg). 1H ΝMR δ (CDCl3): 1.39 (d, 6H), 2.40 (quin, 2H), 3.82 (s, 3H), 4.35 (t, 2H), 4.62 (mult, IH), 4.68 (t, 2H), 6.79 (s, IH), 6.84 (s, IH), 7.20 (s, IH), 7.33 (mult, 2H), 8.60 (s, 2H), 8.97 (s, IH); m/z 437 (M+H)+
The following compound was synthesised in an analogous fashion from 3-hydroxy-5- [(liS}-2-methoxy-(l-methylethyl)oxy]-N-(l-methyl-lH-pyrazol-3-yl)benzamide.
Figure imgf000113_0002
The preparation of 3-hydroxy-5-[(l-methylethyl)oxy]-iV-(l-methyl-lH-pyrazol-3- yl)benzamide and 3 -hydroxy-5-[( 1 iS)-2-methoxy-( 1 -methylethyl)oxy] -N-( 1 -methyl- IH- pyrazol-3-yl)benzamide were described earlier. The preparation of 2-(azetidin-l-ylcarbonyl)-5-bromopyrimidine is described below:
2-(Azetidin- 1 -ylcarbonvD-5 -bromopyrimidine
Figure imgf000114_0001
Oxalyl chloride (1.50 mL, 16.8 mmol) then DMF (2 drops) were added to a mixture of 5- bromopyrimidine-2-carboxylic acid (prepared according to literature procedure described in WO 2005/028452) (2.86 g, 14.0 mmol) in DCM (40 mL). The reaction mixture was stirred at RT for 2 hours, the volatiles removed in vacuo and the residue dissolved in DCM (40 mL). Azetidine hydrochloride (1.44 g, 15.4 mmol) followed by triethylamine (4.29 mL, 30.8 mmol) were added and the mixture stirred at RT for 72 hours. The mixture was concentrated in vacuo and ethyl acetate (100 mL) added to the residue. The organics were washed with water (100 mL), citric acid solution (50 mL), saturated sodium bicarbonate solution (50 mL), brine (50 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give a yellow solid which was chromatographed on silica, eluting with a gradient of 50- 100% ethyl acetate in isohexane, to give the desired compound (0.86 g). 1H NMR δ (CDCl3): 2.39 (quin, 2H), 4.32 (t, 2H), 4.63 (t, 2H)5 8.92 (s, 2H); m/z 242, 244 (M+H)+
Example 14; S-lfό-fAzetidin-l-ylcarbonvDpyridin-S-ylloxyl-S-IFI-fluoro-l- (fluoromethvπethvnoxy|-iV-(l-methyl-lH-pyrazol-3-vπbenzamide
Figure imgf000114_0002
A mixture 3- { [2-fluoro- 1 -(fluoromethyl)ethyl]oxy } -5-hydroxy-N-( 1 -methyl- lH-pyrazol-3- yl)benzamide (100 mg, 0.32 mmol), 2-(azetidin-l-ylcarbonyl)-5-bromopyridine (86 mg, 0.35 mmol), cesium carbonate (209 mg, 0.64 mmol) and bromotris(triphenylphosphine)copper(I) (150 mg, 0.16 mmol) in DMA (5 mL) was stirred in a 'Biotage initiator Microwave' at 16O0C for 3 hours. The reaction mixture was added to ethyl acetate (50 mL) and water (50 mL), the organic layer washed with brine (50 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give a brown oil. The residue was chromatographed on silica, eluting with 0-100% ethyl acetate in isohexane, to give a colorless oil which appeared to be a mixture of product and eliminated product (3-{[6- (azetidin- 1 -ylcarbonyl)pyridin-3-yl]oxy } -5- { [ 1 -(fluoromethyl)ethenyl]oxy } -N-( 1 -methyl- lH-pyrazol-3-yl)benzamide). This mixture was dissolved in chloroform (5 mL), TFA (0.5 niL) added and the mixture stirred for 2 hours. Complete degradation of eliminated product was observed by LCMS and the mixture concentrated in vacuo then water added. The mixture was neutralised and extracted into ethyl acetate (2 x 30 mL), washed with brine (30 mL), dried (MgSO4), filtered and reduced to give a yellow oil which was chromatographed on silica, eluting with 0-100% ethyl acetate in isohexane, to give the desired compound (13 mg).
1H NMR δ (CDCl3): 2.29 (quin, 2Η), 3.76 (s, 3H), 4.19 (t, 2H), 4.55 (mult, 2H), 4.67 (mult, 5H), 6.72 (s, IH), 6.80 (mult, IH), 7.09 (s, IH), 7.25 (mult, 2H), 7.32 (mult, IH), 8.06 (d, IH), 8.26 (d, IH)5 8.43 (s, IH); m/z472 (M+H)+
The preparation of 3 - { [2-fluoro- 1 -(fluoromethyl)ethyl] oxy } -5-hydroxy-N-( 1 -methyl- IH- pyrazol-3-yl)benzamide and 2-(azetidin-l-ylcarbonyl)-5-bromoρyridine were described earlier.
Example 15: 3-{r5-(azetϊdm-l-ylcarbonyl)pyrazin-2-ylloxy}-5-{f2-fluoro-l- (fluoromethvDethylloxyl-TV-d-methyl-lg-pyrazoI-S-vDbenzamide
Figure imgf000115_0001
DIPEA (0.45 mL, 2.58 mmol) was added to a mixture of 5-[(3-{[2-fluoro-l- (fluoromethyl)ethyl]oxy } -5- { [( 1 -methyl- lH-pyrazol-3- ytyarmnojcarbonyljphenytyoxyjpyrazine^-carboxylic acid (0.28 g, 0.65 mmol), azetidine hydrochloride (121 mg, 1.29 mmol) and ΗATU (516 mg, 1.36 mmol) in DMF (5 mL) and stirred at RT for 72 hours. Ethyl acetate (40 mL) was added and the organics washed with water (2 x 30 mL), brine (30 mL), dried (MgSO4), filtered and reduced in vacuo to give a yellow oil which was chromatographed on silica, eluting with 0-100% ethyl acetate in isohexane, to give the desired compound (109 mg). 1H NMR δ (CDCl3): 2.32 (quin, 2H)3 3.70 (s, 3H), 4.19 (t, 2H), 4.54 - 4.72 (m, 7H), 6.73 (s, IH), 6.93 (t, IH), 7.22 (d, IH), 7.25 (s, IH), 7.34 (s, IH), 8.28 (d, IH), 8.78 (d, IH), 8.80 (s, IH); m/z 473 (M+H)+
The following compound was synthesised in an analogous fashion from 5-[(3-[(l- methylethyl)oxy]-5-{[(l-methyl-lH-pyrazol-3-yl)amino]carbonyl}phenyl)oxy]pyrazine-2- carboxylic acid.
Figure imgf000116_0002
The preparation of 5-[(3-{[2-fluoro-l-(fluoromethyl)ethyl]oxy}-5-{[(l-methyl-lH- pyrazol-3-yl)amino]carbonyl}phenyl)oxy]pyrazine-2-carboxylic acid is described below:
S-rβ-ir∑-Fluoro-l-Cfluoromethvnethylloxyl-S-ird-methyl-lH-pyrazol-S- yl)amino1carbonyUphenyl)oxylpyrazine-2-carboxylic acid
Figure imgf000116_0001
Lithium hydroxide monohydrate (77 mg, 1.82 mmol) in water (2 mL) was added to methyl 5-[(3-{[2-fluoro-l-(fluoromethyl)ethyl]oxy}-5-{[(l-methyl-lH-pyrazol-3- yl)amino]carbonyl}phenyl)oxy]pyrazine-2-carboxylate (325 mg, 0.73 mmol) in TΗF (4 mL) and the mixture stirred at RT for 20 hours. The TΗF was removed in vacuo and the aqueous residue washed with ethyl acetate to remove impurities then acidified with IM citric acid. Ethyl acetate was added and a white solid removed by filtration and dried in vacuo to give the desired compound as a white solid (0.28 g). 1H NMR δ (d6-DMSO): 3.79 (s, 3H), 4.77 (m, 4H), 5.12 (t, IH), 6.59 (s, IH), 7.22 (s, IH), 7.50 (s, IH), 7.62 (s, 2H), 8.69 (s, IH), 8.80 (s, IH), 10.90 (s, IH), 13.50 (s, IH), m/z 434 (M+H)+
5-[(3-[(l-Methylethyl)oxy]-5-{[(l-methyl-lH-pyrazol-3- yl)amino]carbonyl}phenyl)oxy]pyrazine-2-carboxylic acid, used in the preparation of Example 15a, was prepared in an analogous fashion from methyl 5-[(3-[(l- methylethyl)oxy]-5-{[(l-methyl-lH-pyrazol-3-yl)amino]carbonyl}phenyl)oxy]pyrazine-2- carboxylate.
Figure imgf000117_0002
The preparation of methyl 5-[(3-{[2-fluoro-l-(fluoromethyl)ethyl]oxy}-5-{[(l-methyl-lH- pyrazol-3-yl)amino]carbonyl}phenyl)oxy]pyrazine-2-carboxylate is described below:
Methyl 5-1(3-1 r2-fluoro-l-ffluoromethyr)ethyl1oxy)-5-( r(l-methyl-lH-pyrazol-3- yl)amino1carbonyl|phenvDoxy1pyrazine-2-carboxylate
Figure imgf000117_0001
A mixture of 3-{ [2-fluoro-l-(fluoromethyl)ethyl]oxy}-5-hydroxy-N-(l-methyl-lH- pyrazol-3-yl)benzamide (250 mg, 0.8 mmol), methyl 5-chloropyrazine-2-carboxylate (208 mg, 1.20 mmol) and potassium carbonate (222 mg, 1.61 mmol) in acetonitrile (5 mL) was stirred in a 'Biotage initiator Microwave' at 120°C for 3 hours. The solvent was removed in vacuo and the residue dissolved in ethyl acetate (50 mL) and water (50 mL), the organic layer washed with brine (50 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give a yellow oil which was chromatographed on silica, eluting with 0-100% ethyl acetate in isohexane, to give the desired compound (0.325 g). 1H NMR δ (CDCl3): 3.71 (s, 3H), 3.96 (s, 3H), 4.56 (m, 2H), 4.68 (m, 3H), 6.73 (s, IH), 6.95 (s, IH), 7.22 (s, IH), 7.27 (s, IH), 7.36 (s, IH), 8.46 (s, IH), 8.67 (s, IH), 8.67 (s, IH); m/z 448 (M+H)+
Methyl 5-[(3-[(l-methylethyl)oxy]-5-{[(l-methyl-lH-pyrazol-3- yl)amino]carbonyl}ρhenyl)oxy]pyrazine-2-carboxylate, used in Example 15a, was prepared in an analogous fashion from 3-hydroxy-5-[(l-methylethyl)oxy]-N-(l -methyl- IH- pyrazol-3-yl)benzamide.
Figure imgf000118_0002
The preparation of 3-{[2-jEluoro-l-(fluoromethyl)ethyl]oxy}-5-hydroxy-N-(l-methyl- IH- pyrazol-3 -yl)benzamide and 3 -hydroxy-5 - [( 1 -methylethyl)oxy] -N-( 1 -methyl- lH-pyrazol- 3-yl)benzamide were described previously.
Example 16: S-lfS-fAzetidin-l-ylcarbonyD-S-chloropyridin^-vnoxyl-S-ddS)^- f(difluoromethyl)oxyl-l-methylethvUoxy)-7V-(l-methyl-lff-pyrazol-3-vI)benzamide
Figure imgf000118_0001
A mixture of 3-({(15)-2-[(difluoromethyl)oxy]-l-methylethyl}oxy)-5-hydroxy-N-(l- methyl-lH-pyrazol-3-yl)benzamide (110 mg, 0.32 mmol), 5-(azetidin-l-ylcarbonyl)-2,3- dichloropyridine (75 mg, 0.32 mmol) and potassium carbonate (89 mg, 0.64 mmol) in acetonitrile (5 mL) was stirred in a 'Biotage initiator Microwave' at 160°C for 3 hours. The solvent was removed in vacuo and ethyl acetate (50 mL) added to the residue which was washed with water (20 mL), brine (5OmL), dried (MgSO4), filtered and the solvent removed in vacuo to give a yellow oil. The residue was chromatographed on silica, eluting with a gradient of 50-100% ethyl acetate in isohexane, to give the desired compound (92 mg). 1H NMR δ (CDCl3): 1.40 (d, 3H), 2.41 (quin, 2H), 3.81 (s, 3H), 4.01 (mult, 2H), 4.26 (t, 2H), 4.38 (t, 2H), 4.67 (sextet, IH), 6.29 (t, IH), 6.82 (d, IH), 6.96 (t, IH), 7.27 (t, IH), 7.31 (d, IH), 7.38 (t, IH), 8.18 (d, IH), 8.26 (d, IH), 8.77 (s, IH), m/z 536 (M+H)+
The following compound was made in an analogous fashion from 3-({(15)-2- [(difluoromethyl)oxy] - 1 -methylethyl } oxy)-5-hydroxy-N-( 1 -methyl- lH-pyrazol-3 - yl)benzamide and 5-(azetidin-l-ylcarbonyl)-2-chloropyridine.
Figure imgf000119_0002
The preparation of 5-(azetidin-l-ylcarbonyl)-2,3-dichloropyridine and 5-(azetidin-l- ylcarbonyl)-2-chloropyridine were described earlier. The preparation of 3-({(15)-2-[(difluoromethyl)oxy]-l-methylethyl}oxy)-5-hydroxy-N-(l- methyl-lH-pyrazol-3-yl)benzamide is described below:
3-r((l^)-2-r(Difluoromethvnoxyl-l-methylethvUoxyV5-hvdroxy-N-α -methyl- IH- pyrazol-3 -vDbenzamide
Figure imgf000119_0001
3-({(15)-2-[(Difluoromethyl)oxy]-l-methylethyl}oxy)-iV-(l-methyl-lH-ρyrazol-3-yl)-5- [(phenylmethyl)oxy]benzamide (0.1 g, 0.23 mmol) was dissolved in ethanol (3 mL) and THF (3 mL) and the flask evacuated and purged with argon (3 times). 10% Palladium on carbon (0.01 g) was added and the flask further evacuated and finally purged with hydrogen gas. The reaction mixture was stirred at RT for 20 hours until completion. The reaction mixture was evacuated and purged with nitrogen (3 times). The catalyst was filtered off through celite and the filtrate concentrated in vacuo to give the desired compound (70 mg). 1H NMR δ (CDCl3): 1.28 (d, 3H), 3.71 (s, 3H), 3.80-3.95 (m, 2H), 4.51 (sextet, IH), 5.96-6.36 (t, IH), 6.53 (s, IH), 6.73 (s, IH)3 6.91 (s, IH), 6.96 (s, IH), 7.22 (s, IH), 8.83 (s, IH). m/z 342 (M+H)+.
3-rUl.S)-2-rωifluoromethvnoxy1-l-methylethvnoxyVN-α-methyl-lH-rJyrazol-3-yl)-5- [(phenylmethvDoxylbenzamide
Figure imgf000120_0001
DIPEA (0.198 mL, 1.14 mmol) was added to a mixture of 3-({(l«S)-2- [(difluoromethyl)oxy]-l-methylethyl}oxy)-5-[(phenyhnethyl)oxy]benzoic acid (0.1 g, 0.28 mmol), 3 -amino- 1 -methyl pyrazole (39 mg, 0.4 mmol) and ΗATU (0.227 g, 0.6 mmol) in DMF (3 mL) and stirred at RT for 20 hours. Ethyl acetate (30 mL) was added and the mixture washed with water (30 mL), brine (30 mL), dried (MgSO4), filtered and reduced in vacuo to give a yellow oil which was chromatographed on silica, eluting with a gradient of 0-100% ethyl acetate in isohexane, to give the desired compound (0.1 g). 1B. ΝMR δ (CDCl3): 1.36 (d, 3Η), 3.68 (s, 3H), 3.82-3.95 (m, 2H), 4.48 (sex, IH), 5.00 (s, 2H), 6.19 (t, IH), 6.63 (s, IH), 6.73 (s, IH), 6.93 (s, IH), 7.03 (s, IH), 7.28 (m, IH), 7.35 (m, 5H), 8.59 (s, IH). m/z 432 (M+H)+. 3-({(lS)-2- [(DifluoromethvDoxyi - 1 -methylethyl) oxyV5- rfohenylmethyDoxyibenzoic acid
Figure imgf000121_0001
Lithium hydroxide monohydrate (19 mg, 0.45 mmol) in water (2 mL) was added to methyl 3 -( { ( 1 £)-2- [(difluoromethyl)oxy]- 1 -methylethyl } oxy)-5 - [(phenykαethyl)oxy]benzoate (0.11 g, 0.3 mmol) in THF (4 mL) and the mixture stirred at RT for 20 hours. The THF was removed in vacuo and the aqueous layer adjusted to pH3 with citric acid then extracted into ethyl actetate (2 x 30 mL). The organics were washed with water (30 mL), brine (30 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give the desired compound (0.1 g). 1H NMR δ (d6-DMSO): 1.27 (d, 3H), 4.00 (m, 2H), 4.75 (sextet, IH), 5.15 (s, 2H), 6.72 (t, IH), 7.08 (t, IH), 7.16 (t, IH), 7.41 (m, 5H), 12.95 (s, IH). m/z 351 (M+H)+.
Methyl 3 -( { ( 1 S)-2- FCdifluoromethyDoxyl - 1 -methylethyl ) oxyV 5- ["(phenylmethyDoxylbenzoate
Figure imgf000121_0002
2-(Fluorosulphonyl)difluoroacetic acid (0.239 mL, 2.31 mmol) was added dropwise, with stirring, to a degassed mixture of methyl 3-{[(l>S)-2-hydroxy-l-methylethyl]oxy}-5- [(phenyhnethyl)oxy]benzoate (0.73 g, 2.31 mmol) and copper (I) iodide (88 mg, 0.46 mmol) in acetonitrile (10 mL) at 45°C. The reaction was stirred at 45°C for 24 hours. The solvent was removed in vacuo and ethyl acetate (30 mL) added. The organics were washed with water (30 mL), brine (30 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give a yellow oil which was chromatographed on silica, eluting with a gradient of 0-30% ethyl acetate in isohexane, to give the desired compound (0.11 g). 1H NMR δ (CDCl3): 1.37 (d, 3H)5 3.93 (s, 3H), 4.00 (m, 2H), 4.63 (sextet, IH), 5.10 (s, 2H), 6.28 (t, IH), 6.77 (t, IH), 7.28 (t, IH), 7.41 (m, 6H). m/z 367 (MH-H)+.
Methyl 3 - { |Y 1 £)-2-hvdroxy- 1 -methylethylloxy} -5 - [(phenylmethypoxyibenzoate
Figure imgf000122_0001
Benzyl bromide (1.89 g, 7.20 mmol) was added to a mixture of methyl 3-hydroxy-5-[(15)- 2-hydroxy-l-methylethoxy]benzoate (1.55 g, 6.86 mmol) and potassium carbonate (1.89 g, 0.014 mol) in DMF (16 mL) and the reaction stirred at RT for 20 hours. Ethyl acetate (40 mL) was added and the mixture washed with water (40 mL), saturated sodiumbicarbonate solution (40 mL), brine (40 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give a red oil which was chromatographed on silica, eluting with a gradient of 0-100% ethyl acetate in isohexane, to give the desired compound (1.7 g).
1H NMR δ (CDCl3): 1.30 (d, 3H), 1.95 (m, IH), 3.76 (m, 2H), 3.92 (s, 3H), 4.53 (m, IH), 5.11 (s, 2H), 6.78 (t, IH), 7.25 (m, IH), 7.32 (m, IH), 7.45 (m, 5H). m/z 317 (M+H)+.
Methyl 3 -hydroxy-5 - \( 1 £)-2-hydroxy- 1 -methylethoxy~|benzoate
Figure imgf000122_0002
Trimethylsilyl iodide (115 mL, 0.79mol) was added to a solution of methyl 3-hydroxy-5- [(15)-2-methoxy-(l-methylethyl)oxy]benzoate (38.01 g, 0.158mol) in acetonitrile (500 mL) and stirred for 24 hours. Methanol (300 mL) was added and the reaction stirred for 10 mins. 10% w/v Aqueous sodium thiosulfate pentahydrate (100 mL) was added to the mixture and stirred for 20 mins. The reaction mixture was neutralised with saturated aqueous sodium bicarbonate solution, the organic solvents removed in vacuo, and the product extracted into ethyl acetate (4 x 100 mL). The combined organic layers were dried (MgSO4), filtered and the solvents removed in vacuo. The crude material was crystallised from ethyl acetate to give the title compound (16.80 g). 1R NMR δ (Cl6-DMSO): 1.18 (d, 3H), 3.40-3.55 (m, 2H), 3.80 (s, 3H), 4.35 (sex, IH), 4.80 (t, IH), 6.57 (m, IH), 6.90 (m, 2H), 9.75 (s, IH). m/z 304 (M+H)+
Methyl 3 -Hydroxy-5 - IY 1 ιSr)-2-methoxy-(' 1 -methylethyl)oxy]benzoate
Figure imgf000123_0001
Methyl 3-[(15)-2-methoxy-(l-methylethyl)oxy]-5-{[phenyhnethyl]oxy}benzoate (50.0 g,
0.152 mmol) was dissolved in a mixture of THF:ethanol (600 mL) and the flask evacuated and purged with nitrogen (3 times). 10% Palladium on carbon (5.0 g) was added and the flask further evacuated and finally purged with hydrogen gas. The reaction mixture was stirred at ambient temperature for 20 hours until completion. The reaction mixture was evacuated and purged with nitrogen (3 times). The catalyst was filtered off, and the filtrate concentrated in vacuo to give the desired compound (36.7 g).
1H NMR δ (d6-DMSO): 1.2 (d, 3H), 3.25 (s, 3H), 3.44 (m, 2H), 3.82 (s, 3H), 4.55 (m, IH),
6.6 (s, IH), 6.9 (s, IH), 6.95 (s, IH), 9.8 (s, IH).
The preparation of methyl 3-[(lS)-2-methoxy-(l-methylethyl)oxy]-5-
{[phenylmethyl]oxy}benzoate was described previously.
Example 17: a-πβ-fAzetidin-l-ylcarbonvnpyridiii-S-vIloxyl-S-ddS)^- f(difluoromethyl)oxyl-l-methylethvUoxyV7V-(l-methyl-lH-pyrazoI-3-yl)benzamide
Figure imgf000123_0002
A mixture of3-({(l<S)-2- [(difluoromethyl)oxy] - 1 -methylethyl } oxy)-5 -hydroxy-N-( 1 - methyl- lH-pyrazol-3-yl)benzamide (220 mg, 0.64 mmol), 2-(azetidin-l-ylcarbonyl)-5- bromopyridine (171 mg, 0.71 mmol), cesium carbonate (419 mg, 1.29 mmol) and bromotris(triphenylphosphine)copper(I) (300 mg, 0.32 mmol) in DMA (5 mL) was stirred in a 'Biotage initiator Microwave' at 16O0C for 4 hours. The reaction mixture was added to ethyl acetate (50 mL) and water (50 mL), the organic layer washed with brine (50 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give a brown oil. The residue was chromatographed on silica, eluting with 0-100% ethyl acetate in isohexane, to give the desired compound (102 mg). 1H NMR δ (CDCl3): 1.28 (d, 3H), 2.28 (quin, 2H), 3.68 (s, 3H), 3.90 (mult, 2H), 4.17 (t, 2H), 4.54 (sextet, IH), 4.63 (t, 2H), 6.19 (t, IH), 6.71 (t, IH), 6.73 (s, IH), 7.06 (s, IH), 7.22 (mult, 2H), 7.30 (mult, IH), 8.04 (d, IH), 8.24 (d, IH), 8.84 (s, IH); m/z 502 (M+H)+
The preparation of 3-({(liS)-2-[(difluoromethyl)oxy]-l-methylethyl}oxy)-5-hydroxy-N-(l- methyl- lH-pyrazol-3-yl)benzamide and 2-(azetidin-l-ylcarbonyl)-5-bromopyridine were described earlier.
Example 18: 3-1 r5-(Azetidin-l-ylcarbonvnpyrazin-2-ylloxy)-5-({(lS)-2- [(difluoromethvDoxyi-l-methylethylloxy^-N-ri-methyl-lJy-pyrazol-S-vDbenzaiiiide
Figure imgf000124_0001
DIPEA (0.41 mL, 2.33 mmol) was added to a mixture of 5-[(3-({(lS)-2-
[(difluoromethyl)oxy] - 1 -methylethyl } oxy)-5 - { [( 1 -methyl- lH-pyrazol-3 - yl)amino]carbonyl}phenyl)oxy]pyrazine-2-carboxylic acid (0.27 g, 0.58 mmol), azetidine hydrochloride (109 mg, 1.17 mmol) and ΗATU (466 mg, 1.22 mmol) in DMF (5 mL) and stirred at RT for 24 hours. Ethyl acetate (40 mL) was added and the organics washed with water (2 x 30 mL), brine (30 mL), dried (MgSO4), filtered and concentrated in vacuo to give a yellow oil. The residue was chromatographed on silica, eluting with 0-5% methanol in ethyl acetate, to give the desired compound (124 mg). 1H ΝMR δ (CDCl3): 1.28 (d, 3Η), 2.30 (quin, 2H), 3.65 (s, 3H), 3.90 (m, 2H), 4.18 (t, 2H), 4.53 (sextet, IH), 4.61 (t, 2H), 6.19 (t, IH), 6.73 (d, IH), 6.84 (t, IH), 7.18 (m, 2H), 7.28 (s, IH), 8.25 (s, IH), 8.76 (s, IH), 9.14 (s, IH); m/z 503 (M+H)+. The preparation of 5-[(3-({(15)-2-[(difluoromethyl)oxy]-l-methylethyl}oxy)-5-{[(l- methyl-lH-pyrazol-3-yl)amino]carbonyl}phenyl)oxy]pyrazine-2-carboxylic acid is described below:
5-\(3-({(lS)-2-\( DifluoromethvDoxyl- l-met-iylethyl}oxyV5--f IYl -methyl- lH-pyrazol-3- vDaminol carbonyl }rjhenyr)oxylpyrazme-2-carboxylic acid
Figure imgf000125_0001
A mixture of 3-({(15)-2-[(difluoromethyl)oxy]-l-methylethyl}oxy)-5-hydroxy-N-(l- methyl-lH-pyrazol-3-yl)benzamide (220 mg, 0.64 mmol), methyl 5-chloropyrazine-2- carboxylate (112 mg, 0.64 mmol) and potassium carbonate (178 mg, 1.29 mmol) in acetonitrile (5 mL) was stirred in a 'Biotage initiator Microwave' at 1600C for 4 hours. The solvent was removed in vacuo and water (20 mL) added. The mixture was adjusted to pΗ3 with IM citric acid and extracted into ethyl acetate (2 x 50 mL). The combined organics were washed with water (20 mL), brine (50 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give a yellow oil (0.19 g) which appeared to be a mixture of the acid and methyl ester. Lithium hydroxide monohydrate (26 mg, 0.60 mmol) in water (3 mL) was added to the mixture of acid and ester (0.19 g, 0.4 mmol) in THF (6 mL) and the mixture stirred at RT for 72 hours. The organics were removed in vacuo and the residue adjusted to pH3 with IM citric acid, twice extracted into ethyl actetate and the combined organics, washed with water (30 mL), brine (30 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give the desired compound as a yellow solid (0.17 g). m/z 464 (M+H)+
The preparation of 3-({(15)-2-[(difluoromethyl)oxy]-l-methylethyl}oxy)-5-hydroxy-N-(l- methyl- lH-pyrazol-3-yl)benzamide was described earlier. hvdroxy-l-methyIpropyIloxy}-JV-(l-methyI-lH-pyrazol-3-yl)benzamide and 3-{[5- (azetidin-l-ylcarbonvI)-3-chloropyridin-2-ylloxyl-5-{f(l£2SV2-hvdroxy-l- methylpropyli oxy}-N-(l-methvI-lH-pyrazoI-3-yl)benzamide (1 : 1)
Figure imgf000126_0001
A solution of 5-(azetidin-l-ylcarbonyl)-2,3-dichloropyridine (95 mg, 0.44 mmol), a mixture of 3-hydroxy-5-{[(li?,2i?)-2-hydroxy-l-methylpropyl]oxy}-N-(l-methyl- IH- pyrazol-3 -yl)benzamide and 3 -hydroxy-5- { [( 15r,25)-2-hydroxy- 1 -methylpropyl]oxy } -N-( 1 - methyl- lH-pyrazol-3-yl)benzamide (1:1) (129 mg, 0.42 mmol) and potassium carbonate (146 mg, 1.05 mmol) in acetonitrile (3 mL) was heated in a microwave reactor at 16O0C for 5 hours. The acetonitrile was removed in vacuo and the residue dissolved in ethyl acetate (25 mL). The organic solution was washed with water (25 mL), brine (25 mL), dried (MgSO4) and evaporated to a residue which was chromatographed on alumina, eluting with a gradient of 0-5% methanol in ethyl acetate, to give the required product (56 mg).
1H ΝMR δ (CDCl3): 1.18 (m, 6H), 2.32 (m, 3H), 3.66 (s, 3H), 3.79 (quin, IH), 4.15 (m, 3H), 4.28 (t, 2H), 6.72 (d, IH), 6.85 (t, IH), 7.17 (s, IH), 7.19 (s, IH), 7.29 (s, IH), 8.08 (m, IH), 8.15 (m, IH), 9.10 (s, IH); m/z 501 (MH-H)+ This mixture of diasteromers were separated by chiral preparatory HPLC on a Chiralpak IA (250mm x 20mm) No. EGO 14 column, eluting with a mixture of isohexane / ethyl acetate / acetic acid / triethylamine (40 / 60 / 0.2 / 0.1) to give the first eluted isomer (43 mg), Example 19a, and the second eluted isomer (40 mg), Example 19b.
The preparation of 5-(azetidin-l-ylcarbonyl)-2,3-dichloropyridine was described earlier.
The preparation of 3-hydroxy-5-{ [(li?,2i?)-2-hydroxy-l-methylpropyl]oxy}-N-(l-methyl- lH-pyrazol-3-yl)benzamide and 3-hydroxy-5-{[(15',2>S)-2-hydroxy-l-methylpropyl]oxy}- N-(I -methyl- lH-pyrazol-3-yl)benzamide (1:1) is described below: 3-Hydroxy-5-{ |Yli?,2ff)-2-hydroxy- 1 -methylpropyl]oxy J-N-(I -methyl- lH-pyrazol-3- ypbenzamide and 3 -hydroxy-5- ( f ( 1 S.2S)-2-hv(koxy- 1 -methylpropyll oxyl -N-( 1 -methyl- lH-pyrazol-3-yr)benzamide (1:1)
Figure imgf000127_0001
A solution of a mixture of 3-{[(li?,2i?)-2-hydroxy-l-methylpropyl]oxy}-N-(l-methyl-lH- pyrazol-3-yl)-5-[(phenylmethyl)oxy]benzamide and 3-{ [(15l,2.S)-2-hydroxy-l- methylpropyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)-5-[(phenylmethyl)oxy]benzamide (1 : 1) (1.26 g, 3.19 mmol) and 10% by weight palladium on carbon (0.13 g) in ethanol (50 mL) was allowed to stir at RT under a hydrogen atmosphere for 16 hours. The solution was filtered through Celite® and washed through with methanol (100 mL). The solution was concentrated in vacuo to give the desired compound (1.03g). 1H ΝMR δ (d6-DMSO): 1.09 (d, 3H), 1.17 (d, 3H), 3.76 (m, IH), 3.78 (s, 3H), 4.34 (quin, IH), 6.48 (t, IH), 6.56 (d, IH), 6.93 (t, IH), 7.05 (t, IH), 7.60 (d, IH), 9.66 (s, IH), 10.67 (s, IH); m/z 306 (M+H)+
3-ir(l.R.2JRV2-hvdroxy-l-methylpropynoxy>-N-(l-methyl-lH-pyrazol-3-ylV5-
Figure imgf000127_0002
methyl-lH-pyrazol-3-ylV5-r(phenylmethvDoxylbenzamide (1:1)
Figure imgf000127_0003
A solution of a mixture of 3-{[(li?,2i?)-2-hydroxy-l-methylpropyl]oxy}-5- [(phenyhnethyl)oxy]benzoic acid and 3-{ [(l£,2S)-2-hydroxy-l-methylpropyi]oxy}-5-
[(phenylmethyl)oxy]benzoic acid (1:1) (2.50 g, 7.92 mmol), l-methyl-lH-pyrazol-3-amine (1.54 g, 15.8 mmol), ΗATU (3.92 g, 10.3 mmol) and DIPEA (2.76 mL, 15.8 mmol) in DMF (15 mL) was stirred at RT and under ambient atmosphere for 16 hours. Water (150 mL) was added and the solution extracted with ethyl acetate (250 mL). The ethyl acetate layer was washed with brine and dried (MgSO4), and evaporated to a residue which was chromatographed on silica, eluting with 50% ethyl acetate in hexane, to give the desired product (1.26 g). 1H NMR δ (CDCl3): 1.17 (s, 3H), 1.18 (s, 3H), 2.44 (d, IH), 3.70 (s, 3H), 3.77 (m, IH), 4.10 (quin, IH), 4.99 (s, 2H), 6.64 (t, IH), 6.75 (d, IH), 6.96 (t, IH), 7.03 (t, IH), 7.22 (d, IH), 7.31 (m, 5H), 8.68 (s, IH); m/z 396 (M+H)+
3-(r(li?,2i?V2-Hvdroxy-l-methylpropylloxy}-5-r(rjhenylmethvDoxy1benzoic acid and 3- {[ri^'^^^-hvdroxy-l-methylpropylioxyl-S-fφhenylmethyDoxyibenzoic acid π:!')
Figure imgf000128_0001
To a solution of a mixture of methyl 3-{[(li?,2i?)-2-hydroxy-l-methylpropyl]oxy}-5- [(phenylmethyl)oxy]benzoate and methyl 3-{[(l.S',25)-2-hydroxy-l-methylpropyl]oxy}-5- [(phenyknethyl)oxy]benzoate (1:1) (2.52 g, 7.63 mmol) in THF (40 mL) was added a solution of lithium hydroxide monohydrate (0.80 g, 19.07 mmol) in water (10 mL). The mixture was allowed to stir at RT for 16 hours. The THF was removed in vaccuo and the resulting solution was partitioned between water (100 mL) and ethyl acetate (250 mL). The ethyl acetate layer was washed with brine (50 mL) and dried (MgSO4). The aqueous layer was then adjusted to pH 7 by addition of IM hydrochloric acid and extracted with ethyl acetate (75 mL). The ethyl acetate layer was washed with brine and dried (MgSO4). The ethyl acetate layers were combined and evaporated to give the required product (2.50 g). 1H NMR δ (CDCl3): 1.18 (s, 3H)5 1.20 (s, 3H), 3.80 (quin, IH), 4.14 (quin, IH), 5.01 (s, 2H), 6.72 (t, IH), 7.21 (m, IH), 7.32 (m, 6H).
Methyl S-lfdiϊ^.R'l^-hydroxy-l-methylpropylioxyl-S-rrphenylmethyDoxylbenzoate and methyl 3 - { IY 1 S2S)-2-h.ydvoxy- 1 -methylpropylioxy I -5- [(phenylmethypoxylbenzoate ( 1 : 1)
Figure imgf000128_0002
A solution of methyl 3-hydroxy-5-{[phenylmethyl]oxy}benzoate (3.00 g, 11.61 mmol), (2i?,3.S)-2,3-dimethyloxirane (3.04 mL, 34.8 mmol), and potassium carbonate (4.02 g, 29.0 mmol) in acetonitrile (60 mL) was heated in a microwave reactor at 150°C for 3 hours. The acetonitrile was removed in vacuo and the residual oil dissolved in ethyl acetate (50 mL), washed with water (50 mL), brine (50 mL), dried (MgSO4) and evaporated to a residual oil. The residue was chromatographed on silica, eluting with ethyl acetate, to give the desired compound (2.52 g). 1H NMR δ (CDCl3): 1.17 (d, 6H), 3.82 (s, 3H), 4.04 (q, 2H), 4.99 (s, 2H), 6.67 (t, IH), 7.14 (s, IH), 7.30 (m, 6H); m/z 330 (M-H)-
The preparation of methyl 3-hydroxy-5-{[phenylmethyl]oxy}benzoate was described earlier.
Example 20: S-ffS-fAzetϊdin-l-vIcarbonvD-S-chloropyridin-l-ylloxyl-iV-d-ethvI-lH- Pyrazol-3-yl)-5-([(liy)-l-methvI-2-(methvIoxy)ethyl]oxy)benzamide
Figure imgf000129_0001
A solution of N-(l-ethyl-lH-pyrazol-3-yl)-3-hydroxy-5-{[(l.S)-l-methyl-2- (methyloxy)ethyl]oxy}benzamide (128 mg, 0. 4 mmol) and the 5-(azetidin-l-ylcarbonyl)- 2,3-dichloropyridine (111 mg, 0.48 mmol) in acetonitrile (2 mL) containing potassium carbonate (111 mg, 0.8 mmol) was heated in a microwave reactor at 16O0C for 6 hours. The reaction mixture was filtered and the filtrate evaporated to dryness under reduced pressure and purified by chromatography on silica, eluting with 50-100% ethyl acetate in hexane to give the required product as a white foam (176 mg).
1R ΝMR δ (CDCl3): 1.28 (d, 3Η), 1.41 (t, 3H), 2.32 (quin, 2H), 3.35 (s, 3H), 3.49 (m, 2H)3 4.02 (q, 2H), 4.17 (t, 2H), 4.30 (t, 2H), 4.56 (q, IH), 6.76 (d, IH), 6.88 (t, IH), 7.18 (s, IH), 7.28 (d, IH), 7.32 (t, IH), 8.09 (d, IH), 8.18 (d, IH), 8.65 (s, IH); m/z 514 (M+H)+
The preparation of N-(l-ethyl-l/f-ρyrazol-3-yl)-3-hydroxy-5-{[(lS)-l-methyl-2- (methyloxy)ethyl]oxy}benzamide is described below: N-ri-Ethyl-lH-pyrazol-3-ylV3-hvdroxy-5-(rπ^-l-methyl-2- (methyloxylethylloxylbenzamide
Figure imgf000130_0001
10% Palladium on carbon (1.9 g, 50% wet) was added under argon to N-(l-ethyl-lH- pyrazol-3-yl)-3-{[(15)-l-methyl-2-(methyloxy)ethyl]oxy}-5-
[(phenylmethyl)oxy]benzamide (19.1 g, 46.7 mmol) in dry TΗF (100 mL) and ethanol (100 mL). The reaction mixture was degassed, placed under a hydrogen balloon and stirred for 16 hours. The mixture was filtered through diatomaceous earth and the filtrate was evaporated to give a brown oil. The residue was purified by column chromatography on silica, eluting with 40-65% ethyl acetate in hexanes, to give the desired product as a clear oil which crystallised on standing (11.35 g).
1H ΝMR δ (CDCl3): 1.21 (d, 6Η), 1.38 (t, 3H), 3.32 (s, 3H), 3.39 - 3.51 (m, 3H), 3.98 (q, 2H), 4.44 - 4.51 (m, IH), 6.54 (s, IH), 6.72 (d, IH), 6.92 (s, 2H), 7.26 (d, IH), 8.18 (s, IH), 8.85 (s, IH); m/z 320 (M+H)+ 318 (M-H)"
N-(I -Ethyl- lH-pyrazol-S-ylVS-Uri^-l-methyl^-Cmethyloxy ethvnoxyl-S- rfphenylmethylloxyibenzamide
Figure imgf000130_0002
ΗATU (23.5 g, 61.83 mmol) was added to 3-[(lS)-2-methoxy-(l-methylethyl)oxy]-5- {[phenylmethyl]oxy}benzoic acid (16.28 g, 51.53 mmol) followed by addition of DMF (140 mL) and cooled to O0C. 1 -Ethyl- lH-pyrazol-3-amine (6.86 g, 61.8 mmol) was added followed by DIPEA (21.3 mL) and the reaction stirred under argon, at 0°C, for 3 hours. The solvent volume was reduced and the residue was dissolved in ethyl acetate (500 mL), washed with citric acid (200 mL), sodium hydrogen carbonate solution (150 mL) and saturated brine solution (2 x 150 mL). The organic layer was separated and dried (MgSO4), filtered and evaporated. Purification by column chromatography on silica, eluting with 10- 50% ethyl acetate in hexanes, afforded the title compound as a pale yellow oil (19.1 g). 1H NMR δ (CDCl3): 1.23 (d, 3H), 1.38 (t, 3H), 3.33 (s, 3H), 3.42 (dd, IH), 3.50 (dd, IH), 3.97 (q, 2H), 4.49 (sextet, IH), 4.99 (s, 2H), 6.66 (t, IH), 6.75 (d, IH), 6.98 (s, IH), 7.02 (s, IH), 7.26 (d, IH), 7.28 - 7.37 (m, 5H), 8.58 (s, IH); m/z 410 (M+H)+
The preparation of 3-[(15)-2-methoxy-(l-methylethyl)oxy]-5-{ [phenylmethyl]oxy}benzoic acid was described earlier.
The preparation of l-ethyl-lH-pyrazol-3-amine is described in the literature [Chem. Heterocycl. Compd. (Engl. Transl.),! 1, 1975, 212].
Example 21: S-lfe-fAzetidin-l-ylcarbonvDpyridin-S-vnoxyl-N-d-ethvI-Lfir-pyrazoI-S- yl)-5-U(lS)-l-methyl-2-(methvIoxy)ethylloxylbenzamide
Figure imgf000131_0001
A mixture of N-(l-ethyl-lH-pyrazol-3-yl)-3-hydroxy-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}benzamide (0.128g, 0.4 mmol), 2-(azetidin-l-ylcarbonyl)-5- bromopyridine (116 mg, 0..48 mmol), cesium carbonate (392 mg, 1.2 mmol) and bromotris(triphenylphosphine)copper(I) (38 mg, 0.04 mmol) in DMA (5 mL) was stirred in a microwave reactor at 1600C for 4 hours. The product was dissolved in ethyl acetate (20 mL) and water (15 mL) and the layers separated. The organic layer washed with brine (50 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give a brown oil which was chromatographed on silica, eluting with a gradient of 70-100% ethyl acetate in hexane to give the desired material as a clear foam (76 mg). 1H NMR δ (CDCl3): 1.26 (d, 3H), 1.40 (t, 3H), 2.29 (quin, 2H), 3.34 (s, 3H), 3.42 - 3.53 (m, 2H), 4.01 (q, 2H), 4.18 (t, 2H), 4.54 (q, IH), 4.64 (t, 2H), 6.74 (t, 2H), 7.04 (s, IH), 7.22 (s, IH), 7.27 (d, IH), 7.30 (d, IH), 8.04 (d, IH), 8.25 (d, IH), 8.47 (s, IH); m/z 480 (M+H)+ The preparation of N-( 1 -ethyl- lH-pyrazol-3 -yl)-3 -hydroxy-5- { [( 1 S)- 1 -methyl-2- (methyloxy)ethyl]oxy}benzamide and 2-(azetidin-l-ylcarbonyl)-5-bromopyridine was described earlier.
Example 22: B-lfS-fAzetidin-l-vIcarbonvπpyrazin-l-vnoxyl-iV-d-ethvI-lH-pyrazol-S- yl)-5-{[(lS)-l-methyl-2-(methvIoxy)ethylloxy}benzamide
Figure imgf000132_0001
ΗATU (304 mg, 0.80 mmol) was added to 5-[(3-{[(l-ethyl-lH-pyrazol-3- yl)amino]carbonyl}-5-{[(l>S)-l-methyl-2-(methyloxy)ethyl]oxy}phenyl)oxy]pyrazine-2- carboxylic acid (0.4 mmol) and azetidine hydrochloride (75 mg, 0.89 mmol) in DMF (5 mL) followed by addition of DIPEA (0.278 mL, 1.6 mmol) and the reaction was stirred for 16 hours. The reaction mixture was added to ethyl acetate (20 mL), the organic solution washed with water (10 mL), 2N citric acid (10 mL), saturated aqueous sodium hydrogencarbonate (10 mL) and brine (10 mL), then dried (MgSO4), filtered and evaporated under reduced pressure to a gum. The residue was purified by chromatography on silica, eluting with 70-100% ethyl acetate in hexane, to give the desired compound as a white foam (98 mg). 1H NMR δ (CDCl3): 1.27 (d, 3H), 1.40 (t, 3H), 2.31 (quin, 2H), 3.34 (s, 3H), 3.43 - 3.54 (m, 2H), 4.01 (q, 2H), 4.19 (t, 2H), 4.54 (q, IH), 4.62 (t, 2H), 6.73 (d, IH), 6.87 (t, IH), 7.18 (t, IH), 7.27 (d, IH), 7.30 (t, IH), 8.26 (d, IH), 8.49 (s, IH), 8.78
Figure imgf000132_0002
The preparation of 5-[(3-{[(l-ethyl-lH-pyrazol-3-yl)amino]carbonyl}-5-{[(15)-l-methyl- 2-(methyloxy)ethyl]oxy}phenyl)oxy]pyrazine-2-carboxylic acid is described below: P T/GB2006/002471
- 132 -
5-ff3-(r(l-Ethyl-lH-pyrazol-3-vnammolcarbonvn-5-ir(l.Sf)-l-methyl-2- (memγloxy)ethyl1oxy}phenyl)oxylpyrazme-2-carboxylic acid
Figure imgf000133_0001
A solution of N-(l-ethyl-lH-pyrazol-3-yl)-3-hydroxy-5-{[(15)-l-methyl-2- (methyloxy)ethyl]oxy}benzamide (128 mg, 0.4 mmol) and the methyl-5-chloropyrazine-2- carboxylate (83 mg, 0.48 mmol) in acetonitrile (2 mL), containing potassium carbonate (111 mg, 0.8 mmol), was heated in a microwave reactor at 16O0C for 6 hours. The reaction mixture was dissolved in water (10 mL), acidified with 2N citric acid and extracted with ethyl acetate (5 x 20 mL). The organic phase was washed with brine, dried (MgSO4) and evaporated to dryness under reduced pressure to give the desired material a yellow solid (164 mg), which was used without purification in the next step, m/z 442 (M+Η)+
The preparation of N-(l-ethyl-lH-pyrazol-3-yl)-3-hydroxy-5-{[(l,S)-l-methyl-2- (methyloxy)ethyl]oxy}benzamide was described earlier.
Example 23: a-irS-fAzetidin-l-ylcarbonylVS-chloropyridin-Z-ylioxyl-S-IKl-^-Z- hvdroxy-l-methylethvUoxyl-iV-fl-d-methylethyD-Lfif-pyrazol-S-yllbenzamide
Figure imgf000133_0002
3-{ [5-(Azetidin-l -ylcarbonyl)-3-chloropyridin-2-yl]oxy }-5-[((lS)-2-{ [(1 , 1- dimethylethyl)(dimethyl)silyl]oxy } - 1 -methylethyl)oxy]-A/-[l -(I -methylethyl)- lH-pyrazol- 3-yl]benzamide (142 mg, 0.23 mmol) in methanol (2 mL) was stirred with 3.5M hydrochloric acid (0.2 mL, 0.68 mmol) for 1 hour. The solution was neutralised with saturated sodium bicarbonate solution and the organic solvent was removed under reduced pressure. The residue was extracted with ethyl acetate (3 x 10 mL), dried (MgSO4), filtered and the solvent removed under reduced pressure. The residue was purified by chromatography on silica, eluting with 75-100% ethyl acetate in hexane, to give the required product as a white foam (99 mg).
1H NMR δ (CDCl3): 1.25 (d, 3H), 1.41 (d, 6H), 2.32 (quin, 2H), 3.67 - 3.71 (m, 2H), 4.17 (t, 2H), 4.26 - 4.35 (m, 3H), 4.52 (q, IH), 6.73 (s, IH), 6.87 (s, IH), 7.19 (s, IH), 7.30 (s, IH), 7.32 (s, IH), 8.10 (s, IH), 8.18 (s, IH), 8.56 (s, IH); m/z 514 (M+H)+
The preparation of 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-[((15)-2- {[(1,1 -dimethylethyl)(dimethyl)silyl]oxy}-l -methylethyl)oxy]-N-[l -(1-methylethyl)- IH- pyrazol-3-yl]benzamide is described below:
S-frS-CAzetidin-l-ylcarbonvn-S-chloropyridm^-ylloxyl-S-rfd^^-irfl.l- dimethylethyl)(dimethyl)silyll oxy} - 1 -methylethvDoxyi -N-Fl-(I -methylethyp- lH-pyrazol- 3-yllbenzamide
Figure imgf000134_0001
A solution of 3-[((l1S)-2-{[(l,l-dimethylethyl)(dimethyl)silyl]oxy}-l-methylethyl)oxy]-5- hydroxy-N-[l-(l-methylethyl)-lH-pyrazol-3-yl]benzamide (103 mg, 0.24 mmol) and 5- (azetidin-l-ylcarbonyl)-2,3-dichloropyridine (66 mg, 0.29 mmol) in acetonitrile (2 mL), containing potassium carbonate (66 mg, 0.48 mmol), was heated in a microwave reactor at 16O0C for 6 hours. The reaction mixture was filtered and the filtrate evaporated to dryness under reduced pressure and purified by chromatography on silica, eluting with 50-100% ethyl acetate in hexane, to give the required product as a white foam (142 mg). m/z 628 (M+Η)+ 3-[(ri^-2-{fri.l-DimethylethviydimetlivnsilvnoxyM-methylethvnoxyl-5-hvdroxy-N-[l- ( 1 -methylethyl)- lH-pyrazol-3 -ylibenzamide
Figure imgf000135_0001
A solution of 3-[((15)-2-{[(l,l-dimethylethyl)(dimethyl)silyl]oxy}-l-methylethyl)oxy]-N- [l-(l-methylethyl)-lH-pyrazol-3-yl]-5-[(phenylmethyl)oxy]benzamide (1.97 g, 3.77 mmol) and TΗF (70 mL) was evacuated and purged with Argon (x3). Palladium on carbon (10% w/w, 400 mg) was added and reaction mixture was evacuated and finally purged with hydrogen gas. Reaction mixture was left to stir at ambient temperature under hydrogen for 16 hours. The palladium on carbon was filtered off and concentrated in vacuo to give the product as a colourless oil (1.58 g, 97%).
1H ΝMR δ (CDCl3): 0.02 (s, 3Η), 0.04 (s, 3H), 0.85 (s, 9H), 1.27 (d, 3H), 1.53 (s, 3H), 1.55 (s, 3H), 3.63 (dd, IH), 3.77 (dd, IH), 4.41 (m, IH), 6.60 (s, IH), 6.81 (s, IH), 7.00 (s, IH), 7.07 (s, IH), 7.38 (s, IH), 8.78 (br. s, IH); m/z 434 (M+H)+, 432 (M-Hy.
S-rfd^-Σ-ird.l-Dimethylethylϊdimethvnsilylloxyl-l-methylethvnoxyi-N-π-d- methylethvD- lH-pyrazol-3 -yll -5- [(phenylmethvDoxyibenzamide
Figure imgf000135_0002
DIPEA (3.11 mL, 18.03 mmol) was added to a solution of 3-[((lS)-2-{[(l,l- dimethylethyl)(dimethyl)silyl]oxy}-l-methylethyl)oxy]-5-[(phenylmethyl)oxy]benzoic acid (3.00 g, 7.21 mmol), ΗATU (3.12 g, 8.21 mmol) and l-(l-methylethyl)-lH-pyrazol-3- amine (1.13 g, 9.01 mmol) in DMF (10 mL). The resulting mixture was stirred at ambient temperature for 16 hours. The DMF was removed in vacuo. The solvent was evaporated and the residue was dissolved in 5%w/v citric acid (50 mL) and ethyl acetate (30 mL) and diethyl ether (30 mL) and the organic layer was further washed with saturated aqueous sodium bicarbonate solution (30 mL) and brine (30 mL). The organic layer was separated, then dried (MgSO4), filtered and evaporated. Purification by column chromatography, eluting with 1:4 to 1:3 ethyl acetate :hexanes, afforded the title compound as a colourless oil (2.40 g, 65%). 1R NMR δ (CDCl3): 0.01 (s, 3H), 0.03 (s, 3H), 0.86 (s, 9H), 1.24 (d, 3H), 1.49 (s, 3H), 1.51 (s, 3H), 3.64 (dd, IH), 3.78 (dd, IH), 4.39 (m, IH), 4.46 (m, IH), 5.09 (s, 2H), 6.70 (s, IH), 6.78 (s, IH), 7.02 (s, IH), 7.08 (s, IH), 7.35 (m, 6H), 8.32 (br. s, IH); m/z 524 (M+H)+, 522 (M-H)-.
1 -(I -MethylethyD- lH-pyrazol-3 -amine
Figure imgf000136_0001
2-Chloroacrylonitrile (3.41 mL, 42.59 mmol) was added at RT to a stirring solution of N- isopropylhydrazine hydrochloride (4.71 g, 42.6 mmol), potassium carbonate (11.8 g, 85.2 mmol) in water (50 mL). The reaction was warmed to 45°C for 4 hours before cooling back to RT. The aqueous layer was then extracted with ethyl acetate (5 x 30 mL) and the combined organic layers were dried (MgSO4), treated with activated charcoal, filtered and evaporated. The residue was purified by chromatography, eluting with 67%- 100% ethyl acetate in hexanes, to afford the title compound (3.08 g, 58%) as a 6:1 mixture of authentic product to regioisomeric product as an oil. The material was used without further purification. 1H ΝMR δ (CDCl3): 1.42 (m, 6H), 3.58 (br. s, 2H), 4.25 (sept, IH), 5.58 (d, IH), 7.15 (d, IH).
The preparation of 3-[((15)-2-{[(l,l-dimethylethyl)(dimethyl)silyl]oxy}-l- methylethyl)oxy]-5-[(phenylmethyl)oxy]benzoic acid was described earlier.
Example 24: 3-{f5-(Azetidin-l-ylcarbonvπpyridin-2-vnoxyl-5-([(lS)-l-methvI-2- (methyloxy)ethyIloxy)-iV-lH-pyrazol-3-ylbenzamide
Figure imgf000136_0002
A solution of 1,1-dimethylethyl 3-{[(3-hydroxy-5-{[(15)-l-methyl-2- (methyloxy)ethyl]oxy}phenyl)carbonyl]amino}-lH-pyrazole-l-carboxylate (157 mg, 0.4 mmol) and 5-(azetidin-l-ylcarbonyl)-2-chloropyridine (95 mg, 0.48 mmol) in acetonitrile (2 mL), containing potassium carbonate (111 mg, 0.8 mmol), was heated in a microwave reactor at 16O0C for 6 hours. The reaction mixture was filtered and the filtrate evaporated to dryness under reduced pressure and purified by chromatography on silica, eluting with 50-100% ethyl acetate in hexane to give a product which was further purified by chromatography on silica, eluting with 0-8% methanol in ethyl acetate, to give the required product as a white foam (21 mg). 1H NMR δ (CDCl3): 1.25 (d, 3H), 2.30 (quin, 2H), 3.33 (s, 3H), 3.42 - 3.54 (m, 2H), 4.12 - 4.29 (m, 4H), 4.54 (q, IH), 6.77 (s, IH), 6.81 (t, IH), 6.97 (d, IH), 7.20 (s, IH), 7.27 (s, IH), 7.33 (d, IH), 8.01 (d, 2H), 8.28 (d, IH), 10.27 (s, IH); m/z 452 (M+H)+
The preparation of 1,1-dimethylethyl 3-{[(3-hydroxy-5-{[(15)-l-methyl-2- (methyloxy)ethyl]oxy}phenyl)carbonyl]amino}-lH-pyrazole-l-carboxylate is described below:
l.l-Dimethylethyl S-irrS-hvdroxy-S-irri^-l-methyl^- (methyloxy)ethyl1oxy>phenyl)carbonyllamino } - lH-pyrazole- 1 -carboxylate
Figure imgf000137_0001
A solution of 1,1-dimethylethyl 3-[({3-{[(15)-l-methyl-2-(methyloxy)ethyl]oxy}-5- [(phenyhnethyl)oxy]phenyl}carbonyl)amino]-lH-pyrazole-l-carboxylate (23 g, 47.8 mmol) in TΗF (140 mL) and ethanol (140 mL) was evacuated and purged with nitrogen (x3). 10% Palladium on carbon (2.3 g; 10% w/w) was added and reaction mixture was evacuated and finally purged with hydrogen gas. The reaction mixture was left to stir at RT under a hydrogen balloon for 16 hours. The palldium on carbon was filtered through diatomaceous earth and the filtrate concentrated in vacuo to give a white foam (18 g, 97%). 1H NMR δ (d6-DMSO): 1.2 (d, 3H), 1.55 (s, 9H), 3.25 (s, 3H obscured by water peak), 3.4- 3.5 (m, 2H), 4.7 (m, IH), 6.5 (s, IH), 6.95 (d, IH), 7.0 (s, IH), 7.1 (s, IH), 8.2 (d, IH), 9.65 (s, IH), 11.2 (s, br, IH); m/z 392 (M+H)+ l.l-Dimethylethvn-fdS-IITl^-l-methyl-Σ-faethyloxy^ethylloxyl-S- lYphenylmethyDoxylphenyl I carbonvDaminol - lH-pyrazole- 1 -carboxylate
Figure imgf000138_0001
DIPEA (28.5 niL, 164 mmol) was added to a suspension of 3-[(lS)-2-methoxy-(l- methylethyl)oxy]-5-{ [phenylmethyl]oxy}benzoic acid (20.7 g, 65.6 mmol), ΗATU (31.2 g, 82.0 mmol) and 1,1-dimethylethyl 3 -amino- lH-pyrazole-1 -carboxylate (15.0 g, 82.0 mmol) in DMF (30 mL) and the reaction mixture stirred for 16 hours at RT. Water (250 mL) was added and the reaction mixture extracted with diethyl ether (3 x 150 mL). The organic layer was washed with saturated brine solution and dried (MgSO4). The filtrate was concentrated in vacuo and the residue crystallised on standing. The crystals were washed with isohexane to give to give the desired material as yellow crystals (23.4 g; 73%). m/z 482 (M+Η)+.
1 , 1 -Dimethylethyl 3-amino- lH-pyrazole- 1 -carboxylate
Figure imgf000138_0002
lH-Pyrazol-3-amine (428 mg, 5.15 mmol) was dissolved in DMF (5 mL) at O0C and treated with sodium hydride (206 mg, 5.15 mmol) followed by stirring for a further 30 min. Warmed di-tert-butyl dicarbonate (1.12 g, 5.15 mmol) was then slowly added via syringe over 5 min and the reaction was allowed to warm to room temperature and stirred for a further 2 h. The reaction was taken up in saturated aqueous sodium hydrogencarbonate (50 mL) and ethyl acetate (100 mL). The organic layer was separated then dried (MgSO4), filtered and evaporated. Purification by column chromatography (eluting with 1:1 ethyl acetate :hexanes to neat ethyl acetate) afforded the title compound (117 mg) as a white solid. 1H NMR δ (CDCl3): 1.62 (s, 9H), 4.00 (br. s, 2H), 5.81 (d, IH), 7.82 (d, IH) 1,1 -Dimethylethyl 3-amino-lH-pyrazole-l-carboxylate was also made by reacting a solution of lH-pyrazol-3 -amine in TΗF (1 g in approximately 15.6 mL) with NaΗMDS (2M in THF, 1.05 equivalents) then adding a solution of di-tert-butyl dicarbonate (1 equivalent) in THF (approximately Ig of dicarbonate in 1.9 mL) and stirring at RT. The resulting material was crystallised from a mixture of ethyl acetate and isohexane. The preparation of 3-[(15)-2-methoxy-(l-methylethyl)oxy]-5-{[phenylmethyl]oxy}benzoic acid was described earlier.
Example 25: S-irS-fAzetidin-l-ylcarbonvO-S-chloropyridin-l-ylloxyl-iV-d-methvI- lH-pyrazol-3-yl)-5-f(35)-tetrahvdrofuran-3-yloxylbenzamide
Figure imgf000139_0001
A mixture of 3-hydroxy-N-(l-methyl-lH-pyrazol-3-yl)-5-[(35)-tetrahydrofuran-3- yloxyjbenzamide (80 mg, 0.26 mmol), 5-(azetidin-l-ylcarbonyl)-2,3-dichloropyridine (61 mg, 0.26 mmol) and caesium carbonate (172 mg, 0.53 mmol) in acetonitrile (3 mL) was stirred in a Smith Creator microwave at 16O0C for 3 hours. The solvent was removed in vacuo and ethyl acetate (50 mL) added to the residue. The organic phase was washed with water (20 mL), brine (50 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give a yellow oil which was chromatographed on silica, eluting with a gradient of 0-10% methanol in DCM, to give the title compound as a yellow foam (52 mg). 1HNMR δ (CDCl3): 2.16 - 2.24 (2Η, m), 2.34 - 2.44 (4H, m), 3.78 (3H, s), 3.89 - 4.01 (4H, m), 4.24 (2H, t), 4.37 (2H, t), 4.99 (IH, s), 6.80 (IH, s), 6.89 (IH, s), 7.23 (IH, s), 7.30 (2H, d), 8.16 (IH, s), 8.24 (IH, s), 8.73 (IH, s); m/z 498 (M+H)+.
The preparation of 5-(azetidin-l-ylcarbonyl)-2,3-dichloropyridine was described earlier. The preparation of 3-hydroxy-N-(l-methyl-lH-pyrazol-3-yl)-5-[(35)-tetrahydrofuran-3- yloxy]benzamide is described below: S-Hydroxy-N-Cl-methyl-lH-pyrazol-B-ylVS-rβiS^-tetrahvdrofiiran-S-yloxylbenzamide
Figure imgf000140_0001
N-(l-Methyl-lH-pyrazol-3-yl)-3-[(phenylmethyl)oxy]-5-[(35)-tetrahydrofuran-3- yloxy]benzamide (453 mg, 1.15 mmol) was dissolved in ethanol (5 mL) and ammonium formate (182 mg, 2.88 mmol) was added in one portion. The reaction was blanketed with argon and 10% palladium on activated carbon (30 mg) was added. This mixture was heated to 140° C for 10 minutes in a Smith Creator microwave. The catalyst was filtered off and the volatiles removed in vacuo to give the title product as a white solid (339 mg). 1H ΝMR δ (CDCl3): 2.06 - 2.14 (IH, m), 2.15 - 2.22 (IH, m), 3.72 - 3.73 (3H, s), 3.84 - 3.89 (IH, m), 3.92 - 3.98 (3H, m), 4.88 (IH, m), 6.53 (IH, t), 6.78 (IH, d), 6.89 (IH, s), 6.95 (IH, s), 7.28 (IH, d), 9.27 (IH, s); m/z 304 (M+H)+.
N-α-Methyl-lH-pyrazol-S-ylVS-rrphenylmethvπoxyi-S-rCS^-tetrahvdrofuran-S- yloxyibenzamide
Figure imgf000140_0002
A suspension of 3-hydroxy-N-(l-rnethyl-lH-pyrazol-3-yl)-5-
[(phenylmethyl)oxy]benzamide (450 mg, 1.39 mmol), (3i?)-tetrahydrofuran-3-yl 4- methylbenzenesulfonate (507 mg, 2.09 mmol) and potassium carbonate (481 mg, 3.48 mmol) in acetonitrile (5 mL) was stirred in a Smith Creator microwave at 1600C for 3 hours. The solvent was removed in vacuo and ethyl acetate added. The organics were washed with water (40 mL), brine (40 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give a yellow foam which was chromatographed on silica, eluting with a gradient of 0-100% ethyl acetate in zso-hexane, to give the title compound as a white foam (452 mg). 1H NMR δ (CDCl3): 2.09 - 2.14 (IH, m), 2.14 - 2.24 (IH, m), 3.68 (3H, s), 3.86 - 3.91 (IH, m), 3.94 - 3.98 (3H, m), 4.89 (IH, s), 5.03 (2H, s), 6.64 (IH, t), 6.85 (IH, s), 6.96 (IH, d), 7.07 (IH, t), 7.27 (IH, m), 7.33 - 7.41 (5H, m), 9.31 (IH, s); m/z 394 (M+H)+.
(3i?VTetrahydrofuran-3 -yl 4-methylbenzenesulfonate
Figure imgf000141_0001
4-Toluene sulfonyl chloride (1.65 g, 8.63 mmol) was added to a solution of R-3- hydroxytetrahydrofuran (0.8 g, 9.08 mmol) and pyridine (0.88 mL, 10.9 mmol) in DCM (15 mL). The reaction was stirred at RT for 72 hours. Water (10 mL) and IM hydrochloric acid (1 mL) were added and the mixture extracted with DCM (15 mL). The organic layer was washed with brine (20 mL), dried (MgSO4), filtered and reduced in vacuo to give a yellow oil which was chromatograped on silica, eluting with a gradient of 0-50% ethyl acetate in isohexane, to give the desired compound (1.0 g). 1H NMR δ (CDCl3): 2.13 (m, 2H), 2.47 (s, 3H), 3.80-3.95 (m, 4H), 5.15 (m, IH), 7.37 (d, 2H), 7.81 (d, 2H).
S-Hvdroxy-N-d-methyl-lH-pyrazol-S-ylVS-rrphenylmetfaylioxylbenzamide
Figure imgf000141_0002
A suspension of N-(l-methyl-lH-pyrazol-3-yl)-3,5-bis[(phenylmethyl)oxy]benzamide (1.0 g, 2.42 mmol) was dissolved in ethanol (12 mL) and ammonium formate (229 mg, 3.63 mmol) was added in one portion. The reaction was blanketed with argon and 10% palladium on activated carbon (10 mg) was added. This mixture was heated to 140°C for 5 minutes in a Smith Creator microwave. The catalyst was filtered off and the volatiles removed in vacuo, the residue was chromatographed on silica, eluting with a gradient of 30-100% ethyl acetate in wo-hexane, to give the title compound as a white solid (378 mg). 1H ΝMR δ (d6-DMSO): 3.78 (3Η, s), 5.13 (2H, s), 6.55 - 6.57 (2H, m), 6.99 (IH, s), 7.17 (IH, s), 7.34 - 7.48 (5H, m), 7.60 (IH, d), 9.74 (IH, s), 10.70 (IH, s); m/z 324 (M+H)+. N-d-Methyl-lH-pyrazol-S-ylVS.S-bisrfphenylmethvDoxylbenzamide
Figure imgf000142_0001
Oxalyl chloride (7.71 mL, 89.7 mmol) was added dropwise to a suspension of 3,5- dibenzyloxybenzoic acid (20.0 g, 59.8 mmol) in DCM (0.5 L) under argon. The reaction was stirred at RT for 6 hours after which time the volatiles were removed in vacuo. The residue was taken up in DCM (300 mL) and a solution of l-methyl-lH-pyrazol-3-amine (5.81 g, 59.8 mmol) in DCM (50 mL) was added dropwise. The resulting solution was stirred for 16 hours at RT after which time a precipitate had formed. The solid was isolated by filtration and recrystallised from ethanol to give the title compound as a white solid (14.8 g). 1H ΝMR δ (d6-DMSO): 3.84 (3H, s), 5.17 (4H, s), 6.59 (IH, d), 6.84 (IH, t), 7.33 - 7.46 (12H, m), 7.62 (IH, d), 10.83 (IH, s); m/z 414 (M+H)+.
Example 26 : 3-{ f 6-(Azetidin-l-ylcarbonyr)pyridin-3-vIl oxy}-iV-(l-methyl-lH-pyrazoI- 3-yl)-5-f(3S)-tetrahvdrofuran-3-yloxylbenzamide
Figure imgf000142_0002
A mixture of 3-hydroxy-N-(l-methyl-lH-pyrazol-3-yl)-5-[(35)-tetrahydrofuran-3- yloxy]benzamide (80 mg, 0.26 mmol), 2-(azetidin-l-ylcarbonyl)-5-bromopyridme (64 mg, 0.26 mmol), bromotris(triphenylphosphine)copper(I) (49 mg, 0.05 mmol) and caesium carbonate (257 mg, 0.78 mmol) in acetonitrile (3 mL) was stirred in a Smith Creator microwave at 1600C for 6 hours. The solid was filtered off and the solvent was removed in vacuo and ethyl acetate (50 mL) added to the residue. The organic phase was washed with water (20 mL), brine (50 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give a yellow oil which was chromatographed on silica, eluting with a gradient of 0-10% methanol in DCM, to give the title compound as a colourless solid (58 mg). This compound was crystallised from ethyl acetate and isohexane using vapour diffusion techniques. 1H NMR δ (CDCl3): 2.11 - 2.17 (IH, m), 2.20 - 2.29 (IH, m), 2.32 - 2.39 (2H, m), 3.78 (3H, s), 3.88 - 3.93 (IH, m), 3.96 - 4.02 (3H5 m), 4.25 (2H, t), 4.71 (2H, t), 4.97 (IH, t), 6.73 (IH, t), 6.79 (IH, s), 7.11 (IH, s), 7.22 (IH, s), 7.27 - 7.30 (IH, m), 7.36 - 7.39 (IH, m), 8.12 (IH3 d), 8.32 (IH, d), 8.73 (IH, s); m/z 464 (M+H)+.
The preparation of 3-hydroxy-N-(l-methyl-lH-pyrazol-3-yl)-5-[(3iS)-tetrahydrofuran-3- yloxy]benzamide and 2-(azetidin-l-ylcarbonyl)-5-bromopyridine was described earlier.
Example 27: 3-ff5-(Azetidin-l-vIcarbonyl)pyrazϊn-2-yl1oxy}-iV-(l-inethyl-lH-pyrazoI- 3-yl)-5-[(3S)-tetrahvdrofuran-3-yloxy1benzamide
Figure imgf000143_0001
A mixture of 3-hydroxy-N-(l-methyl-lH-pyrazol-3-yl)-5-[(3iS)-tetrahydrofuran-3- yloxy]benzamide (80 mg, 0.26 mmol), 2-(azetidin-l-ylcarbonyl)-5-chloropyrazine (52 mg, 0.26 mmol) and caesium carbonate (172 mg, 0.53 mmol) in acetonitrile (3 mL) was stirred in a Smith Creator microwave at 1600C for 1 hr. The solvent was removed in vacuo and ethyl acetate (50 mL) added to the residue. The organic phase was washed with water (20 mL), brine (50 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give a yellow oil which was chromatographed on silica eluting with a gradient of 0-10% methanol in DCM to give the title compound (36 mg, 31 %) as a white foam. This compound was crystallised from ethyl acetate and isohexane using vapour diffusion techniques. 1H ΝMR δ (CDCl3): 2.12 - 2.19 (IH, m), 2.21 - 2.28 (IH, m), 2.34 - 2.42 (2H, m), 3.75 (3H, s), 3.88 - 3.93 (IH, m), 3.98 - 4.02 (3H, m), 4.23 (2H, t), 4.69 (2H, t), 4.95 - 4.97 (IH, m), 6.80 (IH, d), 6.87 (IH, t), 7.23 (IH, d), 7.27 - 7.30 (IH, m), 7.28 - 7.30 (IH, m), 8.34 (IH, d), 8.84 (IH, d), 8.98 (IH, s); m/z 465 (M+H)+.
The preparation of 3-hydroxy-N-(l-methyl-lH-pyrazol-3-yl)-5-[(3iS)-tetrahydrofuran-3- yloxy]benzamide was described earlier. The preparation of 2-(azetidin-l-ylcarbonyl)-5-chloropyrazine is described below:
2-(Azetidin- 1 -ylcarbonvD-5 -chloropyrazine
Figure imgf000144_0001
Oxalyl chloride (1.55 mL, 17.48 mmol), followed by DMF (2 drops), was added to a mixture of 5-chloropyrazine-2-carboxylic acid (2.31 g, 14.57 mmol) in DCM (40 mL). The reaction was stirred at RT for 2 hours after which time the volatiles were removed in vacuo. The residue was taken up DCM (40 mL) and azetidine (1.08 mL, 16.03 mmol) and triethylamine (4.46 mL, 32.06 mmol) added. The mixture was stirred at RT for 72 hours. The volatiles were removed in vacuo and ethyl acetate (100 mL) added to the residue. The organics were washed with water (100 mL), citric acid (50 mL), saturated sodium bicarbonate solution (50 mL), brine (50 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give a yellow solid. The residue was chromatographed on silica, eluting with a gradient of 50 -100% ethyl acetate in iso-hexane, to give the desired compound as a yellow solid (2.38 g). 1H NMR δ (CDCl3): 2.35 - 2.42 (2H, m), 4.26 (2H, t), 4.67 (2H, t), 8.52 (IH, d), 9.09 (IH, d); m/z 198 (M+H)+.
5-Chloropyrazine-2-carboxylic acid
Figure imgf000144_0002
To a solution of methyl-5-chloropyrazine-2-carboxylate (120 mg, 0.70 mmol) in a mixture of acetonitrile (2 mL) and DMF (1 mL) was added lithium chloride (295 mg, 6.95 mmol). The suspension was heated to 160°C for 5 mins in a Smith creator microwave after which time the reaction was diluted with water (10 mL). Saturated sodium bicarbonate solution (20 mL) was added and the aqueous layered extracted twice with ethyl acetate (30 mL). The combined organics were discarded and the aqueous layer adjusted to pH 4 with IN hydrochloric acid. The aqueous phase was extracted twice with ethyl acetate (20 mL) and the combined organics washed with water (2 x 20 mL), brine (10 mL) and dried (MgSO4). The volatiles were removed to give the title compound as a colourless solid (68 mg). 1E. NMR δ (CDCl3): 7.20 (IH, br s), 8.72 (IH, s), 9.21 - 9.21 (IH, m); m/z 157 (M-H)+. Example 28: 3-(r4-(Azetidin-l-ylcarbonvn-l.,3-thiazol-2-ylloxyl-5-{rflS)-l-methyl-2- (methyloxy)ethylloxyl-iV-(l-methyl-lH-pyrazol-3-vI)benzamide
Figure imgf000145_0001
Cesium carbonate (488 mg, 1.5 mmol) was added to a solution of 3-hydroxy-5-[(15)-2- methoxy-(l-methylethyl)oxy]-N-(l-methyl-lH-pyrazol-3-yl)benzamide (153 mg, 0.5 mmol) and 4-(azetidin-l-ylcarbonyl)-2-bromo-l,3-thiazole (206 mg, 0.75 mmol) in acetonitrile (5 mL) and the stirred mixture heated at 15O0C in a Biotage Initiator Microwave apparatus for 8 hours. The mixture was cooled to RT and pressure, poured onto water (75 mL) and extracted with ethyl acetate (3 x 25 mL). The combined organic layers were washed with brine, dried (MgSO4) and evaporated to a residue which was chromatographed on silica, eluting with ethyl acetate, to give the desired material (95 mg). 1H ΝMR δ (CDCl3): 1.3 (d, 3H), 2.2 (m, 2H), 3.3 (s, 3H), 3.4-3.55 (m, 2H), 3.7 (s, 3H), 4.1 (t, 2H), 4.4 (t, 2H), 4.5 (m, IH), 6.7 (s, IH), 6.95 (s, IH), 7.25 (s, IH), 7.3 (s, 2H), 7.35 (s, IH), 7.65 (s, IH) and 8.8 (s, IH); m/z 472 (M+H)+.
The following compound was prepared in an analogous fashion from 4-(azetidin-l- ylcarbonyl)-2-bromo-l,3-thiazole and 3-hydroxy-5-[(l-methylethyl)oxy]-N-(l-methyl-lH- pyrazol-3 -yl)benzamide
Figure imgf000145_0002
The preparation of 3-hydroxy-5-[(l>S)-2-methoxy-(l-methylethyl)oxy]-N-(l-methyl-lH- pyrazol-3-yl)benzamide and 3-hydroxy-5-[(l-methylethyl)oxy]-N-(l-methyl-lH-pyrazol- 3-yl)benzamide was described earlier 471
- 145 -
The preparation of 4-(azetidin-l-ylcarbonyl)-2-bromo-l,3-thiazole is described below:
4-(Azetidin-l-ylcarbonylV2-bromo-1.3-thiazole
°KNrBr
To a solution of 2-bromothiazole-5-carboxylic acid (752 mg, 3.62 mmol) in DCM (10 mL) was slowly added oxalyl chloride (0.38 mL, 4.34 mmol) and then DMF (1 drop). The mixture was stirred for 4 hours, following which the organics were removed in vacuo, and the residues azeotroped with toluene (10 mL). The crude material was dissolved in DCM (10 mL) and slowly added to a stirred suspension of azetidine hydrochloride (404 mg, 4.34 mmol) and triethylamine (1.8 mL, 13 mmol) in DCM (25 mL). The mixture was stirred at RT for 2 hours before the organics were removed in vacuo. The residue was partitioned between ethyl acetate (50 mL) and water (25 mL), the organic layer washed with brine (25 mL), dried (MgSO4) and evaporated to a residue which was chromatographed on silica, eluting with 40% ethyl acetate in zso-hexane, to give the desired compound (500 mg). 1R NMR δ (CDCl3): 2.3 (m, 2H), 4.15 (m, 2H), 4.6 (m, 2H) and 8.0 (s, IH); m/z 249 (M+H)+.
Example 29: 3-{f5-(Azetidin-l-ylcarbonyl)pyrazin-2-vnoxyl-5-{ \ (IS)-I- (hvdroxymethyl)propyIloxyl-N-(l-methvI-lff-pyrazol-3-yl)benzamide
Figure imgf000146_0001
A solution of 5-[(3-{[(15)-l-(hydroxymethyl)propyl]oxy}-5-{[(l-methyl-lH-pyrazol-3- yl)amino]carbonyl}phenyl)oxy]pyrazine-2-carboxylic acid (12 mg, 0.03 mmol), azetidine hydrochloride (4 mg, 0.06 mmol), ΗATU (14 mg, 0.04 mmol) and DIPEA (0.02 mL, 0.11 mmol) in DMF (1 mL) was stirred at RT over the weekend. Water (15 mL) was added and the solution extracted with ethyl acetate (25 mL). The ethyl acetate layer was washed with brine (20 mL), dried (MgSO4), and evaporated to a residue which was chromatographed by preparative ΗPLC on Cl 8 reversed phase, eluting with 5-95% acetonitrile (+0.2% TFA) in water (+0.2% TFA), to give the required product along with 15% of a co-eluting impurity (5 mg). 1R NMR δ (CDCl3): 0.92 (t, 3H), 0.97 (t, 0.45H), 1.69 (quin, 2H), 1.76 (m, 0.3H), 2.34 (quin, 2H), 3.76 (m, 2H), 3.86 (s, 3H), 4.23 (t, 2H), 4.49 (sextet, IH), 4.66 (t, 2H), 4.77 (m, 0.15H), 6.93 (t, IH), 6.99 (d, IH), 7.34 (s, IH), 7.46 (s, IH), 7.50 (s, IH), 8.29 (s, IH), 8.78 (s, IH), 10.68 (s, IH), 10.78 (s, 0.15H); m/z 468 (M+H)+
The spectroscopic data is consistent with 15% of the following impurity 3-{[5-(azetidin-l- ylcarbonyl)pyrazin-2-yl] oxy } -5- { [(25)-2-hydroxybutyl] oxy } -N-( 1 -methyl- lH-pyrazol-3 - yl)benzamide.
The preparation of 5-[(3-{[(15)-l-(hydroxymethyl)propyl]oxy}-5-{[(l-methyl-lH-pyrazol- 3-yl)amino]carbonyl}phenyl)oxy]pyrazine-2-carboxylic acid is described below:
S-rβ-irri^-l-fΗvdroxymethvDpropylloxyl-S-ird-methyl-lH-pyrazol-S- vDamAnolcarbonvUphenyl)oxylpyrazine-2-carboxylic acid
Figure imgf000147_0001
Lithium hydroxide monohydrate (6 mg, 0.14 mmol) in water (1 mL) was added to a solution of methyl 5-[(3- { [(I S)- 1 -(hydroxyme1hyl)propyl]oxy } -5- { [(I -methyl- lH-pyrazol- 3-yl)amino]carbonyl}phenyl)oxy]pyrazine-2-carboxylate (25 mg, 0.06 mmol) in TΗF (1 mL). The mixture was allowed to stir at RT for 16 hours. The TΗF was removed in vacuo and the resulting solution was partitioned between water (10 mL) and ethyl acetate (25 mL), the ethyl acetate layer was washed with brine (10 mL) and dried (MgSO4). The aqueous layer was then adjusted to pΗ 7 by addition of IM hydrochloric acid and extracted with ethyl acetate (25 mL). The ethyl acetate layer was washed with brine (10 mL) and dried (MgSO4). The ethyl acetate extracts were combined and evaporated to give the required product (12 mg). m/z 428.44 (M+Η)+ Methyl S-rβ-lfd^-l-divdroxymethvnpropylloxyl-S-irri-methyl-lH-pyrazol-S- vDaminol carbonyl }phenvDoxylpyrazine-2-carboxylate
Figure imgf000148_0001
Methyl 5-chloropyrazine-2-carboxylate (104 mg, 0.60 mmol) and cesium carbonate (467 mg, 1.43 mmol) were added to a solution of 3-hydroxy-5-{[(liS)-l-
(hydroxymethyl)propyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide (175 mg, 0.570 mmol), in acetonitrile (5 mL) and the mixture heated in a microwave reactor at 16O0C for 330 minutes. The acetonitrile was removed in vacuo and the residue dissolved in ethyl acetate (25 mL), washed with water (20 mL), brine (20 mL), dried (MgSO4) and evaporated to a residue which was chromatographed by preparative ΗPLC on C 18 reversed phase, eluting with 5-95% acetonitrile (+0.2% TFA) in water (+0.2% TFA), to give the required product (41 mg). 1H ΝMR δ (CDCl3): 0.97 (t, 3H), 1.78 (quin, 2H), 3.82 (s, 3H), 3.91 (s, 3H), 4.52 (m, 3H), 4.66 (quin, IH), 6.59 (t, IH), 6.89 (d, IH), 7.05 (t, IH), 7.15 (t, IH), 8.19 (d, IH), 8.79 (d, IH), 9.86 (s, IH); m/z 442 (M+H)+
3-Hydroxy-5-{|Tl»Sf)-l-(hvdroxymethyl)propylloxy>-.A/-(l-methyl-lH-pyrazol-3- vDbenzamide
Figure imgf000148_0002
To a solution of 3-hydroxy-5-({(15)-l-[(methyloxy)methyl]propyl}oxy)-N-(l-methyl-lH- pyrazol-3-yl)benzamide (500 mg, 1.6 mmol) in acetonitrile (25 mL), iodotrimethylsilane (1.11 mL, 7.8 mmol) was added and the resulting mixture stirred for 16 hours. Saturated sodium hydrogencarbonate solution (10 mL) was added, the solution stirred for 10 mins, saturated aqueous sodium thiosulfate (5 mL) was added then the acetonitrile was removed in vacuo. The residual aqueous layer was extracted with ethyl acetate (3 x 40 mL) and the organic layers combined, dried (MgSO4), filtered and evaporated and purified by column chromatography, eluting with 85% ethyl acetate in isohexane, to give the title compound as a colourless foam (405 mg). 1H NMR δ (d6-DMSO): 0.95 (t, 3H), 1.5-1.8 (m, 2H), 3.5 (m, 2H), 3.8 (s, 3H), 4.3 (m, IH), 4.8 (t, IH), 6.45 (s, IH), 6.55 (s, IH), 6.9 (s, IH), 7.05 (s, IH), 7.55 (s, IH), 9.6 (s, IH); m/z 306 (M+H)+
3 -Hydroxy-5-( { ( 1 S)- 1 -[(methyloxy^methylipropyl } oxy)-N-( 1 -methyl- lH-pyrazol-3 - vDbenzamide
Figure imgf000149_0001
To a solution of 3-({(liS)-l-[(methyloxy)methyl]propyl}oxy)-N-(l-methyl-lH-pyrazol-3- yl)-5-[(phenylmethyl)oxy]benzamide (4.6 g, 11 mmol) in 1:1 TΗF methanol (100 mL) was added 10% w/w palladium on carbon (450 mg) and the resulting mixture was stirred under an atmosphere of hydrogen for 6 hours. The atmosphere was replaced with argon and the mixture was filtered and evaporated to afford the title compound as a white solid (3.6 g). 1H ΝMR δ (CDCl3): 0.95 (t, 3H), 1.7 (m, 2H), 3.4 (s, 3H), 3.55 (m, 2H), 3.8 (s, 3H), 4.3 (m, IH), 6.65 (s, IH), 6.8 (s, IH), 7.0 (m, 2H), 7.2 (m, IH), 7.3 (s, IH), 8.7 (s, IH); m/z 320 (M+H)+
3-("(ri^-l-r(Methyloxy)methvnpropyUoxyVN-ri-methyl-lH-pyrazol-3-ylV5- [(phenylmethvDoxylbenzamide
Figure imgf000149_0002
To a solution of 3-({(15)-l-[(methyloxy)methyl]propyl}oxy)-5-
[(phenylmethyl)oxy]benzoic acid (4.75 g, 14.4 mmol) and 3-amino-l-methyl-lH-pyrazole (2.04 g, 21 mmol) in DMF (25 mL) was added ΗATU (8.53 g, 22.4 mmol) then DIPEA (7.0 mL, 40 mmol) and the resulting mixture was stirred for 16 hours. The mixture was partitioned between ethyl acetate (100 mL) and water (30 mL). The organic layer was separated, washed with IN citric acid (30 mL), water (30 mL), saturated sodium bicarbonate (30 mL), water (30 mL) and brine (30 mL) then dried (MgSO4) and evaporated. The residue was purified by column chromatography, eluting with 50% ethyl acetate in isohexane, to give the title compound as a colourless oil (4.57 g). 1H NMR δ (CDCl3): 0.95 (t, 3H), 1.7 (m, 2H), 3.4 (s, 3H), 3.55 (m, 2H), 3.8 (s, 3H), 4.3 (m, IH), 5.05 (s, 2H), 6.75 (s, IH), 6.8 (s, IH), 7.05 (d, 2H), 7.25 (s, IH), 7.4 (m, 5H), 8.45 (s, lH); rø/z 410 (M+H)+
3-({(l^-l-r(Methyloxy)methylipropyUoxyV5-r('rjhenylmethyl')oxylbenzoic acid
Figure imgf000150_0001
To a solution of methyl 3-({(liS)-l-[(methyloxy)methyl]propyl}oxy)-5-
[(phenyhnethyl)oxy]benzoate (6.85 g, 20 mmol) in 3:1 THF:methanol (100 mL) was added IN lithium hydroxide solution in water (40 mL, 40 mmol), then a further 100 mL water was added portionwise at intervals while the resulting mixture was stirred for 2 hours. The organic solvents were removed by evaporation and the cloudy solution filtered. The pH of the filtrate was adjusted to 3 by the addition of 2 M hydrochloric acid. This was extracted with ethyl acetate (3 x 70 mL). The combined organic extracts were dried (MgSO4) and evaporated to afford the title compound as a colourless oil which solidified (6.36 g,). 1U NMR δ (CDCl3): 0.95 (t, 3H), 1.7 (m, 2H), 3.4 (s, 3H)3 3.55 (m, 2H), 4.3 (m, IH), 5.05 (s, 2H), 6.8 (s, IH), 7.3-7.5 (m, 7H); m/z 329 (M-H)-
Methyl 3-(((1SVl- [(methyloxylmethvHpropyl } oxy)-5-rfphenylmethyr)oxy~lbenzoate
Figure imgf000150_0002
A stirred solution of methyl 3-hydroxy-5-{[phenylmethyl]oxy}benzoate (7.5 g, 29 mmol), (iO-l-methoxy-butan-2-ol (3.76 g, 36.25 mmol) and triphenylphosphine (9.5 g, 36.25 mmol) in dry THF (75 mL) was cooled in an ice-bath and a solution of 40% DEAD in toluene (15.8 mL, 36.25 mmol) was added dropwise over 30 minutes. The reaction mixture was allowed to warm slowly to 1O0C and stirred for 16 hours. The THF was evaporated and the residue was dissolved in 30% ethyl acetate in isohexane and cooled in ice. The resultant precipitate was removed by filtration and washed with 10% ethyl acetate in isohexane. The filtrate was evaporated and purified by column chromatography, eluting with 10% ethyl acetate in isohexane, to give the title compound as a colourless oil (6.85 g). 1H NMR δ (CDCl3): 0.95 (t, 3H), 1.7 (m, 2H), 3.35 (s, 3H), 3.55 (m, 2H), 3.9 (s, 3H), 4.3 (m, IH), 5.05 (s, 2H), 6.8 (s, IH), 7.25 (m, 2H), 7.4 (m, 5H); m/z 345 (M+H)+
The preparation of (i?)-l-methoxy-butan-2-ol was described in the literature [Coke, J. L.;
Shue, R. S., J. Org. Chem. 38, (1973), 2210-2211].
The preparation of methyl 3-hydroxy-5-{[phenylmethyl]oxy}benzoate was described earlier.
Example 30: S-lfl-fAzetϊdin-l-ylcarbonvπpyrimidin-S-vnoxyl-S-lfd^-l-methyl-l- (methyloxy)ethylloxyl-iV-1.3-thiazoI-2-vIbenzamide
Figure imgf000151_0001
A mixture of 3-hydroxy-5-{[(lS)-2-methoxy-(l-methylethyl)oxy}-N-l,3-thiazol-2- ylbenzamide (130 mg, 0.42 mmol), 2-(azetidin-l-ylcarbonyl)-5-bromopyrimidine (113 mg, 0.46 mmol), cesium carbonate (412 mg, 1.26 mmol) and bromotris(triphenylphosphine)copper(I) (118 mg, 0.13 mmol) in DMA (5 mL) was stirred in the a microwave reactor at 16O0C for 4 hours. Ethyl acetate (50 mL) was added to the residue and the organics washed with water (20 mL), brine (50 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give a brown oil. The residue was chromatographed on silica, eluting with a gradient of 0-10% methanol in ethyl acetate, to give the desired compound (67 mg). 1H ΝMR δ (CDCl3): 1.34 (d, 3H), 2.38 (quintet, 2H), - ISl -
3.40 (s, 3H), 3.49 - 3.61 (m, 2H), 4.29 (t, 2H), 4.59 - 4.67 (m, 3H), 6.92 (t, IH), 6.98 (d, IH), 7.23 (d, IH), 7.44 (t, IH), 7.49 (t, IH), 8.54 (s, 2H), 11.96 (s, IH); m/z 470 (M+H)+
The preparation of 3-hydroxy-5-{[(15)-2-methoxy-(l-methyleihyl)oxy}-N-l ,3-thiazol-2- ylbenzamide was described earlier.
The preparation of 2-(azetidin-l-ylcarbonyl)-5-bromopyrimidine is described below:
2-fAzetidin-l-ylcarbonylV5-bromopyrimidine
Figure imgf000152_0001
Oxalyl chloride (1.50 rnL, 16.79 mmol), followed by DMF (2 drops), were added to a mixture of 5-bromopyrimidine-2-carboxylic acid (2.86 g, 14.0 mmol) in DCM (40 mL). The reaction was stirred at RT for 2 hours, the volatiles removed in vacuo and the residue dissolved in DCM (40 mL). Azetidine hydrochloride (1.44 g, 15.39 mmol), followed by triethylamine (4.29 mL, 30.78 mmol), were added and the mixture stirred at RT for 72 hours. The mixture was concentrated in vacuo and ethyl acetate (100 mL) added to the residue. The organics were washed with water (100 mL), citric acid (50 mL), saturated sodium bicarbonate solution (50 mL), brine (50 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give a yellow solid. The solid was chromatographed on silica, eluting with a gradient of 50-100% ethyl acetate in isohexane, to give the desired compound as a yellow solid (0.86 g).
The preparation of 5-bromopyrirnidine-2-carboxylic acid is described in the literature (International Patent Application WO 2005/028452).
Example 31: S-lfS-fAzetidin-l-ylcarbonvDpyrazin^-ylloxyl-S-fcvclopentyloxyViV-d- methyl-lff-pyrazol-3-yr)benzamide
Figure imgf000153_0001
3-(Cyclopentyloxy)-5-hydroxy-N-(l-methyl-lH-pyrazol-3-yl)benzamide (200 mg, 0.67 mmol), 2-(azetidin-l-ylcarbonyl)-5-chloropyrazine (159 mg, 0.80 mmol) and potassium carbonate (184 mg, 1.33 mmol) were dissolved / suspended in acetonitrile (3.5 mL). The reaction mixture was heated for 4 hours at 12O0C in a microwave reactor. The mixture was cooled, filtered and concentrated in vacuo. The crude product was chromatographed on silica, eluting with 0-5% methanol in DCM, to give the required product as a white foam (282 mg).
1H ΝMR δ (d6-DMSO): 1.61 (m, 2H), 1.74 (m, 4H), 1.94 (m, 2H), 2.30 (m, 2H), 3.78 (s, 3H), 4.10 (t, 2H), 4.57 (t, 2H), 4.94 (m, IH), 6.57 (d, IH), 7.01 (t, IH), 7.42 (m, IH), 7.47 (m, IH), 7.60 (d, IH), 8.55 (d, IH), 8.68 (d, IH), 10.80 (s, IH). m/z 463 (M+H)+
The following compound was made in an analogus fashion from 3-(cyclopentyloxy)-5- hydroxy-N-(l -methyl- lH-pyrazol-3-yl)benzamide and 5-(azetidin-l-ylcarbonyl)-2,3- dichloropyridine.
Figure imgf000153_0002
The preparations of 2-(azetidin-l-ylcarbonyl)-5-chloropyrazine and 5-(azetidin-l- ylcarbonyl)-2,3-dichloropyridine were described earlier. The preparation of 3-(cyclopentyloxy)-5-hydroxy-N-(l-methyl-lH-pyrazol-3- yl)benzamide is described below: B-CCyclopentyloxyVS-hydroxy-N-ri-πietliyl-lH-pyrazol-S-vDbenzamide
Figure imgf000154_0001
3 -(Cyclopentyloxy)-N-( 1 -methyl- lH-pyrazol-3 -yl)-5 - [(phenylmethyl)oxy]benzamide (1.87g, 4.78 mmol) was dissolved in ethanol (40 mL) and 10% palladium on charcoal (102 mg) catalyst added under argon. The reaction was stirred under an atmosphere of hydrogen for 86 hours, then filtered through Celite® and concentrated in vacuo to a light brown solid (1.3Ig). 'ΗΝMRδ (d6-DMSO): 1.54 (m, 2H), 1.76 (m, 4H), 1.96 (m, 2H), 2.75 (s, IH), 3.83 (s, 3H), 4.91 (m, IH), 6.49 (m, IH), 6.61 (m, IH), 6.98 (m, IH), 7.06 (m, IH), 7.65 (s, IH), 9.73 (br s, IH); m/z 302 (M+H)+
S-rCyclopentyloxyVN-d-methyl-lH-pyrazol-S-ylVS-fCphenylmethyπoxylbenzamide
Figure imgf000154_0002
3-(Cyclopentyloxy)-5-[(phenylmethyl)oxy]benzoic acid (3.14g, 10.0 mmol), 1-methyl-lΗ- pyrazol-3-amine (1.95g, 20 mmol) and HATU (4.95g, 13 mmol) were dissolved in DMF (12.5 mL) then DIPEA (3.49 mL, 20 mmol) was added. The resultant mixture was stirred at RT for 20 hours. The mixture was quenched with water (150 mL) and extracted with ethyl acetate (2 x 75 mL), washed with brine, dried (MgSO4), filtered and concentrated in vacuo to leave a yellow oil. The residue was chromatographed on silica, eluting with with 0-30% ethyl acetate in isohexane, to give the desired product as a clear gum (1.87g). 1H ΝMR 5 (CDCl3): 1.59 (m, 4H), 1.83 (m, 4H), 3.79 (s, 3H), 4.76 (m, IH), 5.08 (s, 2H), 6.66 (t, IH)5 6.82 (m, IH), 7.01 (m, IH), 7.08 (m, IH), 7.26 (m, IH), 7.33 (m, IH), 7.35- 7.45 (m, 4H), 8.67 (s, IH); m/z 392 (M+H)+ 3-(Cyclopentyloxy)-5-[(phenylmethyr)oxy'1benzoic acid
Figure imgf000155_0001
Methyl 3-(cyclopentyloxy)-5-[(phenylmethyl)oxy]benzoate (9.25g, 28.34 mmol) was dissolved in THF (120 mL) and a solution of lithium hydroxide mono hydrate (3.49 g, 85.0 mmol) in water (60 mL) added. The bi-phasic solution was stirred at RT for 16 hours
(LCMS indicated reaction 80% complete), methanol (15 mL) added and the mixture stirred for a further 4 hours. The THF was removed in vacuo then water (40 mL) added and the pH adjusted to 7 with hydrochloric acid. The solid was collected and washed thoroughly with cold water (8.85 g). 1H NMR δ (d6-DMSO): 1.64 (m, 2H), 1.76 (m, 4H), 1.96 (m, 2H), 4.89 (m, IH), 5.19 (s, 2H), 6.80 (s, IH), 7.07 (s, IH), 7.16 (s, IH), 7.34-7.53 (m, 5H); m/z 311 (M+H)+
Methyl 3-(cyclopentyloxy)-5-[(phenylmethyDoxylbenzoate
Figure imgf000155_0002
Methyl 3-hydroxy-5-{[phenylmethyl]oxy}benzoate (10 g, 38.7 mmol), 1-cyclopentanol (6.135 mL, 58.07 mmol) and triphenylphosphine (15.24g, 58.07 mmol) were stirred under argon in THF (166 mL) and cooled in an ice bath to 50C. DEAD (25.3 mL, 58.1 mmol) was added dropwise to the mixture, maintaining the internal temperature in the range 5 - 1O0C. Stirring was continued for 16 hours. The mixture was concentrated in vacuo, re- dissolved in ethyl acetate (60 mL) and isohexane (60 mL), the resultant precipitate removed and the solution concentrated in vacuo to give a yellow oil. The residue was chromatographed on silica, eluting with 0-30% ethyl acetate in isohexane, to give a colourless oil which crystallised to a white solid under vacuum. 1H NMR δ (CDCl3): 1.62 (m, 2H), 1.71-1.98 (m, 6H), 3.90 (s, 3H), 4.76 (m, IH), 5.08 (s, 2H), 6.69 (m, IH), 7.16 (m, IH), 7.23 (m, IH), 7.29-7.44 (m, 5H); m/z 325 (M+H)+
The preparation of methyl 3-hydroxy-5-{[phenylmethyl]oxy}benzoate was described earlier.
Example 32: 3-{[6-(Azetidin-l-ylcarbonyl)pyridin-3-ylloxy}-5-(cvclopentyloxy)-iV-(l- methyl-ljy-pyrazol-3-yl)benzamide
Figure imgf000156_0001
3-(Cyclopentyloxy)-5-hydroxy-iV-(l-methyl-lH-pyrazol-3-yl)benzamide (200 mg, 0.67 mmol), 2-(azetidin-l-ylcarbonyl)-5-bromopyridine (193 mg, 0.80 mmol), bromotris(triphenylphosphine)copper (186 mg, 0.2 mmol) and caesium carbonate (230 mg, 0.70 mmol) were dissolved / suspended in DMA (3.5 mL). The reaction mixture was heated for 40mins at 2000C in a microwave reactor then allowed to cool, filtered and reduced in vacuo. The crude product was purified by chromatography on silica, eluting with 0-100% ethyl acetate in isohexane, to give the desired compound as a slightly yellow foam (176 mg). 1HNMR δ (d6-DMSO): 1.61 (m, 2H), 1.73 (m, 4H), 1.94 (m, 2H), 2.28 (m, 2H), 3.78 (s, 3H), 4.08 (t, 2H), 4.59 (t, 2H), 4.95 (m, IH), 6.56 (d, IH), 6.88 (t, IH), 7.28 (m, IH), 7.43 (t, IH), 7.56 (m, IH), 7.59 (d, IH), 7.99 (d, IH), 8.41 (m, IH), 10.85 (s, lH); m/z 462 (M+H)+
Example 33 : 3-{ f 5-(Azetidin-l-ylcarbonyl)pyr azin-2-yli oxyl-54 r(lR.,21f)-2-hvdroxy-l- methylpropylloxy}-Λ^l-methyl~l/r-pyrazol-3-yl)benzamide and S-US-fAzetidin-l-ylcarbonvnpyrazin-Σ-vIloxyl-S-πflSJS^-l-hvdroxy-l- methylpropyll oxyl-JV-d-methyl-lH-pyr azol-3-yl)benzamide (1 : 1)
Figure imgf000157_0001
DIPEA (0.26 mL, 1.5 mmol) was added to a mixture of 5-[(3-{[(li?,2i?)-2-hydroxy-l- methylpropyl] oxy } -5 - { [( 1 -methyl- lH-pyrazol-3 -yl)amino]carbonyl }phenyl)oxy]pyrazine- 2-carboxylic acid and 5-[(3-{[(15',21S)-2-hydroxy-l-methylpropyl]oxy}-5-{[(l-methyl-lH- pyrazol-3-yl)amino]carbonyl}phenyl)oxy]pyrazine-2-carboxylic acid (1:1) (0.16 g, 0.37 mmol), azetidine hydrochloride (71 mg, 0.75 mmol) and ΗATU (299 mg, 0.79 mmol) in DMF (5 mL) and stirred at RT for 72 hours. Ethyl acetate (40 mL) was added and the mixture washed with water (2 x 30 mL), brine (30 mL), dried (MgSO4), filtered and reduced in vacuo to give a yellow oil which was chromatographed on silica, eluting with 0- 100% ethyl acetate in isohexane to give the titled mixture of diasteroisomers as a colourless oil. The mixture was separated by chiral preparatory ΗPLC on a Chiralpak IA (250 mm x 20 mm) EGO 14 column, eluting with 30% ethanol in tertbutylmethyl ether. The first diastereomer eluted, Example 33a, was isolated as a white foam (18 mg). 1R NMR δ (CDCl3): 1.19 (d, 3Η), 1.23 (d, 3H), 2.26 - 2.34 (m, 3H), 3.74 (s, 3H), 3.77 - 3.85 (m, IH), 4.13 - 4.23 (m, 3H), 4.63 (t, 2H), 6.72 (d, IH), 6.86 (t, IH), 7.16 - 7.22 (m, 2H), 7.27 - 7.30 (m, IH), 8.27 (d, IH), 8.31 (s, IH), 8.79 (d, IH); m/z 467 (M+H)+
The second diastereomer eluted, Example 33b, was isolated as a white foam (19 mg). 1HNMR δ (CDCl3): 1.19 (d, 3H), 1.22 (d, 3H), 2.27 - 2.36 (m, 3H), 3.73 (s, 3H), 3.76 - 3.84 (m, IH), 4.13 - 4.22 (m, 3H), 4.62 (t, 2H), 6.72 (d, IH)5 6.86 (t, IH), 7.17 - 7.19 (m, IH), 7.21 (d, IH), 7.28 (t, IH), 8.26 (d, IH), 8.40 (s, IH), 8.79 (d, IH); m/z 461 (M+H)+
The preparation of a mixture of 5-[(3-{[(li?,2i?)-2-hydroxy-l-methylpropyl]oxy}-5-{[(l- methyl-lH-pyrazol-3-yl)amino]carbonyl}ρhenyl)oxy]pyrazine-2-carboxylic acid and 5- [(3-{[(l,S',25)-2-hydroxy-l-methylproρyl]oxy}-5-{[(l-methyl-lH-pyrazol-3- yl)amino]caxbonyl}phenyl)oxy]pyrazine-2-carboxylic acid (1:1) is described below:
5-rr3-{r(li?,2i?V2-Ηvdroxy-l-methylpropylloxyl-5-irri-methyl-lH-t)yrazol-3- yl)aminolcarbonvUphenyπoxy]pyrazine-2-carboxylic acid and
5-1(3- { TC 1 ■S',2Sr)-2-Ηvdroxy- 1 -methylpropylioxyl -5-1 IY 1 -methyl- lH-pyrazol-3- vDamino1carbonyl|phenyDoxylpyrazine-2-carboxylic acid (1:1")
Figure imgf000158_0001
Lithium hydroxide monohydrate (38 mg, 0.88 mmol) in water (3 mL) was added to methyl 5-[(3-{[(li?52i?)-2-hydroxy-l-methylpropyl]oxy}-5-{[(l-methyl-lH-pyrazol-3- yl)ammo]carbonyl}phenyl)oxy]pyrazine-2-carboxylate and methyl 5-[(3-{[(lS,2S)-2- hydroxy- 1 -methylpropyl]oxy } -5- { [(I -methyl- lH-pyrazol-3- yl)amino]carbonyl}phenyl)oxy]pyrazine-2-carboxylate (1:1) (0.26 g, 0.59 mmol) in TΗF (6 mL) and the mixture stirred at RT for 72 hours. The TΗF was removed in vacuo and the aqueous residue adjusted to pΗ3 with citric acid, then extracted into ethyl actetate, washed with water (30 mL), brine (30 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give the desired compound as a white solid (0.16 g). 1H NMR δ (d6-DMSO): 1.16 (d, 3H), 1.26 (d, 3H), 3.84 (s, 3H), 4.20 (m, IH), 4.49 (m, IH), 4.89 (d, IH), 6.64 (d, IH), 7.13 (t, IH), 7.49 (s, IH), 7.59 (s, IH), 7.67 (d, IH), 8.72 (d, IH)5 8.85 (d, IH), 10.93 (s, IH), 13.53 (s, IH); m/z 426 (M-H)" Methyl 5-[G- { \(lR2K)-2-hv(koxy- 1 -methylpropylloxy>-5- ( IY 1 -methyl- lH-pyrazol-3- yDamino] carbonyl lphenyDoxylpyrazine-2-carboxylate and
Methyl 5-|Y3-{ [(lff.ΣSVΣ-hvdroxy-l-methylpropylioxyl-S-l r(l-methyl-lH-pyrazol-3- vDamino]carbonyl|phenvDoxylpyrazme-2-carboxylate (1:1)
Figure imgf000159_0001
A mixture of 3-hydroxy-5-{[(li?,2i?)-2-hydroxy-l-methylpropyl]oxy}-N-(l-methyl-lH- pyrazol-3 -yl)benzamide and 3 -hydroxy-5 - { [( 15',2>S)-2-hydroxy- 1 -methylpropyl]oxy } -N-(I- methyl- lH-pyrazol-3-yl)benzamide (1:1) (230 mg, 0.75 mmol), methyl 5-chloropyrazine- 2-carboxylate (195 mg, 1.13 mmol) and potassium carbonate (208 mg, 1.51 mmol) in acetonitrile (5 mL) was stirred in a microwave reactor at 130°C for 3 hours. The solvent was removed in vacuo and water added. The mixture was acidified, extracted into ethyl acetate and the combined organics washed with brine, dried (MgSO4), filtered and reduced in vacuo to give a yellow foam (265 mg), which was used in the next step without further purification. 1H ΝMR δ (CDCl3): 1.29 (m, 6H), 1.99 (s, IH), 3.80 (s, 3H), 3.90 (quintet, IH), 4.05 (s, 3H), 4.25 (quintet, IH), 6.82 (d, IH), 6.95 (t, IH), 7.30 (m, 2H), 7.39 (s, IH), 8.55 (s, IH), 8.55 (s, IH), 8.86 (s, IH); m/z AAl (M+H)+
The preparation of 3 -hydroxy-5 - { [( li?,2i?)-2-hydroxy- 1 -methylpropyl]oxy } -N-( 1 -methyl- lH-pyrazol-3-yl)benzamide and 3-hydroxy-5-{[(l.S,25)-2-hydroxy-l-methylpropyl]oxy}- N-(l-methyl-lH-pyrazol-3-yl)benzamide (1:1) was described earlier. Example 34: S-irS-fAzetidin-l-ylcarbonvIVlJ-thiazol-Σ-vIloxyl-S-ird^-l-methyl-Z- (methyloxy)ethvll oxv}τΛ^5HmethvIpvrazin-2-vr)benzamide
Figure imgf000160_0001
Cesium carbonate (489 mg, 1.5 mmol) was added to a solution of 3-hydroxy-5-{[(15)-l- methyl-2-(methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide (159 mg, 0.5 mmol) and 5-(azetidin-l-ylcarbonyl)-2-chloro-l,3-thiazole (152 mg, 0.75 mmol) in acetonitrile (5 mL) and the stirred mixture heated at 1200C in a microwave reactor for 1 hour. The mixture was cooled to RT and ambient pressure, the acetonitrile evaporated in vacuo, the residue partitioned between water (25 mL) and ethyl acetate (50 mL), the organic layer washed with brine, dried (MgSO4) and evaporated to a residue which was chromatographed on silica, eluting with ethyl acetate, to give the desired product (181 mg). 1H ΝMR δ (CDCl3): 1.3 (d, 3H), 2.35 (m, 2H), 2.5 (s, 3H), 3.3 (s, 3H), 3.45 - 3.55 (m, 2H), 4.1 - 4.4 (m, 4H), 4.55 (m, IH), 7.0 (d, 1H),7.35 (d, IH), 7.45 (s, IH), 7.45 (s, IH), 8.05 (s, IH), 8.4 (s, IH) and 9.45 (s, IH). m/z 484 (M+H)+.
The following compounds were synthesised in an analogous fashion from either 3- hydroxy-5-{[(lιS)-l-methyl-2-(methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide or 3 -hydroxy-5-[( 1 >S)-2-methoxy-( 1 -methylethyl)oxy] -N-( 1 -methyl- lH-pyrazol-3 - yl)benzamide and the appropriate halogenated heterocycle:
Figure imgf000160_0002
4.2
8.3 IH).
(d, 8.05
2H), -3.55 (d, and
2H), 3.7 (s,
(s,
Figure imgf000161_0001
*** Example 34b, 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{[(15)-l-methyl-2- (methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide, was also prepared in the following manner.
A mixture of 3-hydroxy-5-{[(15)-l-methyl-2-(methyloxy)ethyl]oxy}-N-(5-methylpyrazin- 2-yl)benzamide (25.4 g, 80.0 mmol), 2-(azetidin-l-ylcarbonyl)-5-chloropyrazine (17.4 g, 88.0 mmol) and potassium carbonate (33.1 g, 240 mmol) in acetonitrile (254 mL) was heated at 6O0C for 24 hours. The solvent was removed by evaporation and the residue partitioned between ethyl acetate (15 vol) and water (10 vol). The layers were separated, the aqueous layer further extracted into ethyl acetate (2 x 10 vol) and the combined organics washed with water (2 x 5 vol), brine (5 vol), dried (MgSO4), filtered, concentrated in vacuo. The residue was chromatographed on silica, eluting with ethyl acetate, to give the desired compound as a foam (33.7 g). The pure material was dissolved in hot tert- butylmethyl ether (30 vols), seeded with genuine crystalline sample (the seeds were isolated from a small scale crystallisation where material was dissolved in a minimum amount of hot fert-butylmethyl ether then isohexane added and the flask scratched to promote crystallisation) and stirred overnight. The mixture was cooled and the crystalline material (melting onset 110.30C) collected by filtration (29.1 g).
1H NMR δ (de-DMSO): 1.27 (d, 3H), 2.30 (quin, 2H), 2.49 (s, 3H), 3.31 (s, 3H), 3.47 - 3.56 (m, 2H), 4.10 (t, 2H), 4.57 (t, 2H)5 4.79 (td, IH), 7.13 (t, IH), 7.48 (td, IH), 7.57 (td, IH), 8.36 (d, IH), 8.56 (d, IH), 8.69 (d, IH), 9.26 (d, IH), 11.00 (s, IH); m/z 479 (M+H)+ This crystalline form could be converted to a different crystalline form by placeing approximately 30mg of material in a vial with a magnetic flea, and adding approximately 1 mL of water. A few (3) drops of methanol were added to this, and the vial was sealed with a lid. The slurry was then left to stir on a magnetic plate at RT (25°C). After 3 days, the sample was removed from the plate, the lid removed and the slurry left to dry under ambient conditions. The new crystalline form (melting onset 110.20C) was shown to be different by XRPD analysis.
The title compound may also be crystallised by stirring a slurry of the compound in water. The preparation of 3-hydroxy-5-{[(l.S)-l-methyl-2-(methyloxy)ethyl]oxy}-N-(5- methylpyrazin-2-yl)benzamide is described below:
3 -Hvdroxy-5- ( \ (IS)-I -methyl-2-fmethyloxy)ethyl1oxy> -N-f 5-methylt>yrazm-2- vDbenzamide
Figure imgf000162_0001
10% Palladium on charcoal (700 mg) was added to a solution of 3-{[( IS)-I -methyl-2- (methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)-5-[(phenylmethyl)oxy]benzamide (7.0 g, 17.2 mmol) in ethanol (125 mL) and the mixture stirred at RT under a hydrogen atmosphere for 4 hours. The catalyst was removed by filtration and the ethanol evaporated in vacuo. The residue was crystallised from ethyl acetate to give the desired compound (4.22 g). 1H NMR δ (CDCl3): 1.25 (d, 3H), 2.5 (s, 3H), 3.3 (s, 3H), 3.4 - 3.5 (m, 2H), 4.5 (m, IH), 6.3 (br, IH), 6.55 (s, IH), 6.9 (s, IH), 6.95 (s, IH), 8.05 (s, IH), 8.45 (s, IH) and 9.5 (s, lH). /n/z 318 (M+H)+.
This reaction was also carried out using methanol as solvent and using 5 bar pressure of hydrogen.
3-{rri^-l-Methyl-2-Cmethyloxy)ethyl1oxyl-N-r5-methylpyrazin-2-vD-5- [(phenylmethvDoxylbenzamide
Figure imgf000163_0001
Oxalyl chloride (2.1 mL, 24.0 mmol) was added to a solution of 3-[(lS)-2-methoxy-(l- methylethyl)oxy]-5-{[phenylmethyl]oxy}benzoic acid (6.32 g, 20.0 mmol) in DCM (100 mL) and the mixture stirred at RT for 4 hours. The mixture was evaporated in vacuo to a residue, which was taken up in DCM (25 mL) and added to a stirred mixture of 2-amino-5- methylpyrazine (2.29 g, 21.0 mmol) and pyridine (1.94 mL, 24.0 mmol) in DCM (100 mL) at 50C - 100C. The mixture was stirred at RT for 18 hours, the DCM evaporated in vacuo. The residue was partitioned between water (50 mL) and ethyl acetate (150 mL), the organic layer washed with brine, dried (MgSO4) and evaporated to a residue, which was chromatographed on silica, eluting with 50% ethyl acetate in isohexane, to give the desired compound (7.0 g). 1H ΝMR δ (CDCl3): 1.3 (d, 3H), 2.5 (s, 3H), 3.3 (s, 3H), 3.4 - 3.5 (m, 2H), 4.5 (m, IH), 5.0 (s, 2H), 6.7 (s, IH), 7.0 (s, IH), 7.05 (s, IH), 7.35 (m, 5H), 8.05 (s, IH), 8.3 (s, IH) and 9.5 (s, IH). m/z 408 (M+H)+.
The preparation of 2-amino-5 methylpyrazine is described in the literature [Tetrahedron Lett. 2002, 9287]. The preparation of 3-[(15)-2-methoxy-(l-methylethyl)oxy]-5-{[phenylmethyl]oxy}benzoic acid was described earlier.
The preparation of 3-hydroxy-5-[(liS)-2-methoxy-(l-methylethyl)oxy]-N-(l-methyl-lH- pyrazol-3-yl)benzamide was described earlier. The preparations of the halogenated heterocycles 5-(azetidin-l-ylcarbonyl)-2-chloro-l,3- thiazole, 5-(azetidin- 1 -ylcarbonyl)-2,3-dichloropyridine, 2-(azetidin- 1 -ylcarbonyl)-5- chloropyrazine, 5-(azetidin-l-ylcarbonyl)-2-chloropyridine and 4-(azetidin-l-ylcarbonyl)- 2-bromo-l,3-thiazole were described earlier.
The preparation of 5-(azetidin-l-ylcarbonyl)-2-bromo-4-methyl-l,3-thiazole, used in Examples 34e-f, is described below:
5-f Azetidin- 1 -ylcarbonylV2-bromo-4-methyl- 1 ,3 -thiazole
Figure imgf000164_0001
Oxalyl chloride (0.47 niL; 5.41 mmol) was added to a solution of 2-bromo-4-methyl-l,3- thiazole-5-carboxylic acid (1.0 g; 4.5 mmol) in DCM (25 mL). The mixture was stirred at RT for 18 hours, the DCM was evaporated in vacuo, the residue azeotroped with toluene (2 x 5 mL) and added to a solution of azetidine hydrochloride (503 mg; 5.41 mmol) and triethylamine (2.3 mL; 16.2 mmol) in DCM (50 mL). The mixture was stirred at RT for 18 hours, the DCM evaporated in vacuo, and the residue partitioned between water (75 mL) and ethyl acetate (150 mL). The organic layer was washed with brine, dried (MgSO4) and evaporated to a residue which was chromatographed on silica, eluting with 40% ethyl acetate in isohexane, to give a solid which was crystallised from ethyl acetate / isohexane to give the desired compound (490 mg). 1H NMR δ (CDCl3): 2.35 (m, 2H), 2.55 (s, 3H) and 4.1 (t, 4H). Example 35: S-lfS-fAzetidin-l-ylcarbonylVS-chloropyridin^-vIloxyl-S-rfl- methylethyl)oxyl-7V-(5-methvIpyrazin-2-vI)benzamide
Figure imgf000165_0001
Cesium carbonate (489 mg, 1.5 mmol) was added to a solution of 3-hydroxy-5-[(l- methylethyl)oxy]-N-(5-methylpyrazin-2-yl)benzamide (144 mg, 0.5 mmol) and 5- (azetidin-l-ylcarbonyl)-2,3-dichloropyridine (173 mg, 0.75 mmol) in acetonitrile (5 mL) and the stirred mixture heated at 1200C in a microwave reactor for 1 hour. The mixture was cooled to RT and ambient pressure, the acetonitrile evaporated in vacuo, the residue partitioned between water (25 mL) and ethyl acetate (50 mL). The organic layer was washed with brine, dried (MgSO4) and evaporated to a residue which was chromatographed on silica, eluting with 75% ethyl acetate in isohexane, to give the desired compound (167 mg). 1H ΝMR δ (CDCl3): 1.3 (d, 6H), 2.3 (m, 2H), 2.5 (s, 3H), 4.1 - 4.3 (m, 4H), 4.55 (m, IH), 6.85 (d, IH), 7.2 (d, IH), 7.3 (s, IH), 8.05 (s, IH), 8.1 (s, IH), 8.2 (s, IH) 8.3 (s, IH) and 9.45 (s, IH). m/z 482 (M+H)+.
The following compounds were prepared in an analogues fashion from 3-hydroxy-5-[(l- methylethyl)oxy]-N-(5-methylpyrazin-2-yl)benzamide and the appropriate halogenated heterocycle:
Figure imgf000165_0002
35b 448 1HNMR δ (CDCl3): 1.3 (d, 6H), 2.3 (m, 2H), (M+H)÷ 2.5 (s, 3H), 4.15 (t, 2H), 4.3 (t, 2H), 4.55 (m, IH), 6.8 (d, IH), 6.95 (d, IH), 7.2 (d, IH), 7.25 (d, IH), 8.0 (dd, IH), 8.1 (s, IH), 8.35 (s, IH) 8.4 (s, IH) and 9.45 (s, IH).
35c* 468 1H NMR δ (DMSO (I6): 1.3 (d, 6H), 2.25 (m, (M+H)+ 2H), 2.45 (s, 3H), 3.3 (s, 3H), 4.0 -4.2 (br, 4H), 4.8 (m, 1H),7.2 (d, IH), 7.6 (d, 2H), 8.35 (s, IH), 9.25 (s, IH) and 11.0 (s, IH).
Figure imgf000166_0001
* Compound was crystallised from ethyl acetate and isohexane; mpt: melting onset 140.40C.
The preparation of 3-hydroxy-5-[(l-methylethyl)oxy]-N-(5-methylpyrazin-2-yl)benzamide is described below:
3-Hydroxy-5-rπ-methylethyl)oxy1-N-r5-methylpyrazin-2-yl)benzamide
Figure imgf000166_0002
10% Palladium on charcoal (550 mg) was added to a solution of 3-[(l-methylethyl)oxy]-N- (5-methylpyrazin-2-yl)-5-[(phenylmethyl)oxy]benzamide (5.5 g, 14.6 mmol) in ethanol (75 mL) and THF (50 mL) and the mixture stirred at RT under a hydrogen atmosphere for 24 hours. The catalyst was removed by filtration and the filtrates evaporated in vacuo to a residue which was crystallised from ethyl acetate to give the desired compound (3.42g). 1R ΝMR δ (CDCl3): 1.3 (d, 6H), 2.5 (s, 3H), 4.5 (m, IH), 5.8 (br, IH), 6.5 (d, IH), 6.9 (d, IH), 6.95 (d, IH), 8.05 (s, IH), 8.4 (s, IH) and 9.5 (s, IH). m/z 288 (M+H)+. 3-rd-MethylethvDoxyl-N-(5-methylρyrazin-2-yl)-5-r(phenylmethvDoxy1benzamide
Figure imgf000167_0001
Oxalyl chloride (2.1 mL, 24.0 mmol) was added to a solution of 3-[(l-methylethyl)oxy]-5- [(phenylmethyl)oxy]benzoic acid (5.72 g, 20.0 mmol) in DCM (100 mL) and the mixture stirred at ambient RT for 4 hours. The mixture was evaporated in vacuo to a residue, which was taken up in DCM (25 mL) and added to a stirred mixture of 2-amino-5-methylpyrazine (2.29 g, 21.0 mmol) and pyridine (1.94 mL, 24.0 mmol) in DCM (100 mL) at 50C - 1O0C. The mixture was stirred at RT for 18 hours, the DCM evaporated in vacuo to a residue, which was partitioned between water (50 mL) and ethyl acetate (150 mL). The organic layer was washed with brine, dried (MgSO4) and evaporated to a residue which was chromatographed on silica, eluting with 30% ethyl acetate in isohexane, and crystallised from ethyl acetate / isohexane to give the desired compound (5.52 g). 1R ΝMR δ (CDCl3): 1.3 (d, 6H), 2.5 (s, 3H), 4.5 (m, IH), 5.0 (s, 2H), 6.6 (s, IH), 6.95 (s, IH), 7.0 (s, IH), 7.35 (m, 5H), 8.05 (s, IH), 8.3 (s, IH) and 9.5 (s, IH). m/z 378 (M+H)+.
The preparations of 3-[(l-methylethyl)oxy]-5-[(phenylmethyl)oxy]benzoic acid and 2- amino-5-methylpyrazine were described earlier.
The preparations of the halogenated heterocycles 5-(azetidin-l-ylcarbonyl)-2,3- dichloropyridine, 2-(azetidin- 1 -ylcarbonyl)-5 -chloropyrazine, 5 -(azetidin- 1 -ylcarbonyl)-2- chloropyridine and 5-(azetidin-l-ylcarbonyl)-2-bromo-4-methyl-l,3-thiazole were described earlier. Example Sόi S-irθ-fAzetidin-l-vIcarbonvnpyridin-a-vnoxyl-S-irflS^-l-methyl-Σ- (methvIoxy)ethyIloxy)-iV-(5-methvIpyrazin-2-yl)benzamide
Figure imgf000168_0001
Cesium carbonate (489 nig, 1.5 mmol) was added to a solution of 3-hydroxy-5-{[(liS)-l- niethyl-2-(methyloxy)ethyl]oxy}-iV-(5-methylpyrazin-2-yl)benzamide (159 mg, 0.5 mmol) and 2-(azetidin-l-ylcarbonyl)-5-bromopyridine (181 mg, 0.75 mmol) and bromotris(triphenylphosphine)copper (93 mg, 0.1 mmol) in DMA (5 mL) and the stirred mixture heated at 1600C in a microwave reactor for 4 hours. The mixture was cooled to RT and ambient pressure, partitioned between water (75 mL) and ethyl acetate (50 mL), the organic layer washed with brine, dried (MgSO4) and evaporated in vacuo. The residue was chromatographed on silica, eluting with ethyl acetate, to give the desired compound that was crystallised from ether (45 mg, melting onset 113.80C). The compound may also be crystallised from ethyl acetate, giving rise to an alternative crystalline form (melting onset 132.70C). 1H NMR δ (CDCl3): 1.3 (d, 3H), 2.3 (m, 2H), 2.5 (s, 3H), 3.3 (s, 3H), 3.45 - 3.55 (m, 2H), 4.2 (t, 2H), 4.55 (m, IH), 4.65 (t, 2H), 6.75 (d, IH), 7.05 (d, IH), 7.25 (s, IH), 7.3 (d, IH), 8.0 (s, IH), 8.05 (d, IH), 8.25 (d, IH), 8.3 (s, IH) and 9.45 (s, IH). m/z 478 (M+H)+.
The preparations of 2-(azetidin-l-ylcarbonyl)-5-bromopyridine and 3-hydroxy-5-{ [(1S)-I- methyl-2-(methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide were described earlier. Example 37 : 3-{ [6-(Azetidin-l-ylcarbonyl)pyridin-3-vn oxyl-5- [(l-methylethvDoxyl -N- (5-methylpyrazin-2-yl)benzamide
Figure imgf000169_0001
Cesium carbonate (489 mg, 1.5 mmol) was added to a solution of 3-hydroxy-5-[(l- methylethyl)oxy]-N-(5-methylpyrazin-2-yl)benzamide (144 mg, 0.5 mmol) and 2- (azetidin-l-ylcarbonyl)-5-bromopyridine (181 mg, 0.75 mmol) and bromotris(triphenylphosphine)copper (93 mg, 0.1 mmol) in DMA (5 mL) and the stirred mixture heated at 16O0C in a microwave reactor for 4 hours. The mixture was cooled to RT and ambient pressure, partitioned between water (75 mL) and ethyl acetate (50 mL), the organic layer washed with brine, dried (MgSO4) and evaporated in vacuo. The residue was chromatographed on silica, eluting with 75% ethyl acetate in isohexane, to give the desired compound (crystallised from ether) (72 mg). 1H ΝMR δ (CDCl3): 1.3 (d, 6H), 2.3 (m, 2H), 2.5 (s, 3H), 4.2 (t, 2H), 4.5 (m, IH), 4.65 (t, 2H), 6.7 (s, IH), 7.05 (s, IH), 7.2 (s, IH), 7.3 (d, IH), 8.0 (br, 2H), 8.3 (br, 2H) and 9.45 (s, IH). m/z 448 (M+H)+. Mpt (melting onset) 125.70C.
The preparations of 2-(azetidin-l-ylcarbonyl)-5-bromopyridine and 3-hydroxy-5-[(l- methylethyl)oxy]-N-(5-methylpyrazin-2-yl)benzamide were described earlier.
Example 38: S-irS-fAzetidin-l-ylcarbonvn-S-chloropyridin-Z-vnoxyl-S-nflS)-!- methyl-2-(methvIoxy)ethvIloxy}-iV-liy-pyrazoI-3-ylbenzamide
Figure imgf000169_0002
A mixture of 1,1-dimethylethyl 3-{[(3-hydroxy-5-{[(lS)-l-methyl-2- (methyloxy)ethyl]oxy}phenyl)carbonyl]amino}-lH-pyrazole-l-carboxylate (196 mg, 0.5 mmol), 5-(azetidin-l-ylcarbonyl)-2,3-dichloropyridine (128 mg, 0.55 mmol) and potassium carbonate (138 mg, 1.0 mmol) in acetonitrile (5 mL) was stirred in a microwave reactor at 12O0C for 4 hours. The solvent was removed in vacuo and the residue partitioned between ethyl acetate and water. The organic layer was washed with brine (50 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give a brown oil which was chromatographed on silica, eluting with a gradient of 50-100% ethyl acetate in isohexane, to give the desired compound as a white foam (142 mg).
1H NMR δ (CDCl3): 1.26 (d, 3H), 2.31 (quin, 2H), 3.33 (s, 3H), 3.43 - 3.53 (m, 2H), 4.11 - 4.33 (m, 4H), 4.54 (q, IH), 6.78 (s, IH), 6.86 (s, IH), 7.24 (s, IH), 7.33 (s, IH), 7.42 (s, IH), 8.06 (d, IH), 8.12 (s, IH), 9.98 (s, IH); m/z 486 (M+H)+
The preparations of 1,1-dimethylethyl 3-{[(3-hydroxy-5-{[(15)-l-methyl-2- (methyloxy)ethyl]oxy}phenyl)carbonyl]amino}-lH-pyrazole-l-carboxylate and 5- (azetidin-l-ylcarbonyl)-2,3-dichloropyridine were described earlier.
Example 39: S-lfS-fAzetidin-l-ylcarbonvIVS-chloropyridin^-ylloxyl-S-UflS)-!- methyl-2-(methyIoxy)ethyI1oxy}-iV-l,3-thiazol-2-vIbenzamide
Figure imgf000170_0001
DIPEA (0.129 mL) was added to a suspension of 3-{[5-(azetidin-l-ylcarbonyl)-3- chloropyridin-2-yl]oxy}-5-{[(liS)-l-methyl-2-(methyloxy)ethyl]oxy}benzoic acid (168 mg), ΗATU (190 mg) and 2-aminothiazole (40 mg) in DMF (2 mL) and the mixture stirred at RT for 16 hours. Water (30 mL) was added and the mixture extracted with ethyl acetate (3 x 20 mL). The combined organic extracts were washed with brine, dried (MgSO4), and evaporated to a residue which was chromatographed on silica, eluting with 50-100% ethyl acetate in isohexane, to give the desired compound (75 mg).
1R NMR δ (CDCl3): 1.27 (d, 3Η), 2.31 (quin, 2H), 3.33 (s, 3H), 3.41 - 3.55 (m, 2H), 4.12 - 4.33 (m, 4H), 4.55 (q, IH), 6.91 (d, IH), 6.95 (s, IH), 7.30 (s, IH), 7.31 (d, IH), 7.40 (s, IH), 8.08 (s, IH), 8.16 (s, IH); m/z 503 (M+H)+ The following compounds were made in an analogous fashion from either 3-{[5-(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl]oxy } -5- { [( 1 S)- 1 -methyl-2- (methyloxy)ethyl]oxy}benzoic acid, 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2- yl]oxy}-5-[(l-methylethyl)oxy]benzoic acid, 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2- yl]oxy}-5-[(l-methylethyl)oxy]benzoic acid, 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2- yl]oxy } -5- { [(I S)- 1 -methyl-2-(methyloxy)ethyl]oxy }benzoic acid, 3- { [6-(azetidin- 1 - ylcarbonyl)pyridin-3-yl]oxy}-5-{ [(liS)-l-methyl-2-(methyloxy)ethyl]oxy}benzoic acid or 3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3 -yl] oxy } -5- [( 1 -methylethyl)oxy]benzoic acid and the appropriate aminoheterocycle.
Figure imgf000171_0001
4.52
(s,
(t,
(d,
(t,
(d,
(quin, 4.67
2H), (s,
(t,
(s,
Figure imgf000172_0001
Figure imgf000173_0001
* The reactions were carried out using 1,1-dimethylethyl 3-amino-lH-pyrazole-l- carboxylate as the amino heterocycle, the resultant products were dissolved in acetonitrile
(4 mL) and ethanol (0.5 mL) and heated in a microwave reactor at 15O0C for 12 minutes.
The mixtures were allowed to cool then concentrated in vacuo to give the desired products.
+ This compound was crystallised from ethyl acetate and isohexane using vapour diffusion techniques.
^ This compound was crystallised from acetonitrile, melting point (melting onset) 108.5
0C.
The preparation of 1,1-dimethylethyl 3-amino-lH-pyrazole-l-carboxylate was described earlier. The preparation of 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-
{[(liS)-l-methyl-2-(methyloxy)ethyl]oxy}benzoic acid is given below: 3- ( [5-f Azetidin- 1 -ylcarbonylVB-chloropyridin-Σ-ylloxyl -5-1 \( IS)-I -methyl-2- (methyloxy'lethylloxylbenzoic acid
Figure imgf000174_0001
A mixture of methyl 3-hydroxy-5-[(15)-2-methoxy-(l-methylethyl)oxy]benzoate (240 mg, 0.1 mmol), 5-(azetidin-l-ylcarbonyl)-2,3-dichloropyridine (278 mg, 1.2 mmol) and potassium carbonate (276 mg, 2.0 mmol) in DMA (5 mL) was stirred at 12O0C for 16 hours. The solution was poured into water (30 mL) and acidified with IN hydrochloric acid before being extracted with ethyl acetate (3 x 20 mL). The combined organics were washed with brine (20 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give methyl 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(15)-l-methyl-2- (methyloxy)ethyl]oxy}benzoate (as evidenced by LCMS data only m/z 435 (M+H)*) as a brownish oil (550 mg). The crude methyl 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2- yl]oxy}-5-{[(15)-l-methyl-2-(methyloxy)ethyl]oxy}benzoate (434 mg,l mmol) was dissolved in THF (15 mL) and methanol (5 mL) then IN aqueous Lithium hydroxide solution (2 mL) added followed by the dropwise addition of water until a clear solution obtained. The mixture was stirred was stirred for 4 hours at RT and the organics removed by evaporation. The aqueous solution was filtered, extracted with ethyl acetate (10 mL) and the aqueous layer acidified with 2N hydrochloric acid. The acidified layer was extracted with ethyl acetate (3 x 30 mL) and the combined organic extracts washed with water (10 mL), brine (10 mL) and concentrated in vacuo to give the desired compound as a pale oil (375 mg). 1R NMR δ (CDCl3): 1.27 (d, 3H), 2.32 (quin, 2H), 3.34 (s, 3H), 3.42 - 3.55 (m, 2H), 4.13 - 4.34 (m, 4H), 4.54 (q, IH), 6.93 (s, IH), 7.40 (s, IH), 7.48 (s, IH), 8.08 (s, IH), 8.19 (s, IH), m/z All (M+H)+
3-{[5-(Azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-[(l-methylethyl)oxy]benzoic acid, 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-[(l-methylethyl)oxy]benzoic acid and 3 - { [5 -(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy } -5- { [( 1 S)- 1 -methyl-2- (methyloxy)ethyl]oxy}benzoic acid were made in an analogous fashion from either methyl 3-hydroxy-5-[(15)-2-methoxy-(l-methylethyl)oxy]benzoate or methyl 3-hydroxy-5-[(l- methylethyl)oxy]benzoate and the appropriate halogenated heterocycle.
-
(t, (t,
Figure imgf000175_0001
The preparation of 3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-[(l- methylethyl)oxy]benzoic acid is described below:
3 - { F6-(Azetidin- 1 -ylcarbonyppyridin-S - ylloxy } -5- IY 1 -methylethvDoxylbenzoic acid
Figure imgf000175_0002
A mixture of methyl 3-hydroxy-5-[(l-methylethyl)oxy]benzoate (0.84 g, 4 mmol), 2-
(azetidin-l-ylcarbonyl)-5-bromopyridine (1.16 g, 4.8 mmol), cesium carbonate (3.92 g, 1.2 mmol) and bromotris(triphenylphosphine)copper(I) (744 mg, 0.8 mmol) in DMA (20 mL) was stirred in a microwave reactor at 160°C for 6 hours. The mixture was diluted with water (100 mL), washed with ethyl actate (2 x 20 mL) and the aqueous layer filtered then acidified with 2N hydrochloric acid. The acidic layer was extracted with ethyl acetate (3 x 50 mL), the combined organics washed with water (2 x 20 mL), brine (20 mL), dried (MgSO4), and the solvent removed in vacuo. The residue was triturated with ether to give the desired compound as a white solid (1.06 g). 1H NMR δ (d6-DMSO): 1.28 (d, 6H), 2.28 (quin, 2H), 4.08 (t, 2H), 4.59 (t, 2H), 4.69 (quin, IH), 6.98 (t, IH), 7.10 (s, IH), 7.28 (s, IH), 7.56 (d, IH), 7.99 (d, IH), 8.41 (s, IH); m/z 357 (M+H)+
3-{[6-(Azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-{[(l.S)-l-methyl-2- (methyloxy)ethyl]oxy}benzoic acid was prepeared in an analogous fashion from 2- (azetidin-l-ylcarbonyl)-5-bromopyridine and methyl 3-hydroxy-5-[(15)-2-methoxy-(l- methylethyl)oxy]benzoate.
Figure imgf000176_0001
The preparations of methyl 3-hydroxy-5-[(15)-2-methoxy-(l-methylethyl)oxy]benzoate, methyl 3-hydroxy-5-[(l-methylethyl)oxy]benzoate and the appropriate halogenated heterocycles were described earlier.
3-{[5-(Azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-[(l-methylethyl)oxy]-N-lH-pyrazol-3- ylbenzamide, Example 39k, was also prepared in the following manner from 1,1- dimethylethyl 3 -[( { 3 -hydroxy-5 -[( 1 -methylethyl)oxy]phenyl } carbonyl)amino] - IH- pyrazole- 1 -carboxylate.
1 , 1 -Dimethylethyl 3-[({3-hydroxy-5-[(l -methylethyl)oxy]phenyl} carbonyl)amino]- IH- pyrazole-1 -carboxylate (56.3 g, 0.16 mol) was dissolved in acetonitrile (500 mL) and charged to a 3L fixed vessel. Potassium carbonate - 325 mesh - (64.5 g, 0.47 mol) was added, followed by 2-(azetidin-l-ylcarbonyl)-5-chloropyrazine (33.5 g, 0.17 mol) with a 100 mL acetonitrile charge wash. The mixture was stirred rapidly and warmed to 60°C under nitrogen. Additional acetonitrile (250 mL) was added followed, after 1.5 hours, by the addition of DMF (150 mL) to dissolve residual solid material. The reaction was stirred overnight then allowed to cool to RT and filtered. The filtrate was concentrated under vacuum to remove the acetonitrile and the residual solution poured into water (1500 mL) with stirring. The precipitated solid was filtered, dissolved in DCM (560 mL), washed with 1:1 brine / saturated aqueous solution of sodium hydrogen carbonate (2 x 500 mL) and dried (MgSO4). The organic solution was filtered and TFA (50 mL). The solution was stirred at RT for 3 hours. Additional TFA (50 mL) was added and the mixture stirred at 30°C for a further 3 hours then at RT overnight. The solvent was removed under vacuum and the residue azeotroped with toluene. The orange oily residue was dissolved in ethyl acetate (500 mL) and washed with a saturated solution of sodium hydrogen carbonate (2 x 500 mL), brine (500 mL), dried (MgSO4) and concentrated to leave a pale yellow waxy solid (64 g). The solid was triturated with ethyl acetate (200 mL) at 45°C for 2 hours, filtered, washed with ethyl acetate and dried in a vacuum oven at 40°C overnight to leave a white solid (52 g). The crude solid was columned on silica, eluting with a mixture of a 7N ammonia in methanol solution in DCM (0.5 - 6.5% methanol gradient), to give pure desired material [pure 1] (23.6 g, 99.1% pure by HPLC) and less pure material recovered from mixed fractions and previous liquors (34.5 g, 82.8% pure by HPLC). The impure material was dissolved in DCM (50 mL) and a white solid crystallised out. The solid was removed by filtration and washed with DCM to give pure desired material [pure 2] (6.5 g, 98.3% pure by HPLC). The filtrate was columned on silica, eluting with 7N ammonia in methanol solution in DCM (0 - 5% methanol gradient), to give the desired material [pure 3] (18.3 g, 98.7% pure by HPLC). Pures 1, 2 & 3 were combined (48.4 g) and charged to a 3L fixed vessel. Ethyl acetate (500 mL, 10 vol) was added and the mixture was heated to reflux with stirring, additional ethyl acetate (400 mL, 8 vol) was added and the remaining solid material removed by hot filtration. The filtrate was cooled to 60°C and isohexane (250 mL) added dropwise. The slurry was cooled to 2O0C over approx 1 hour and then stirred at RT overnight. The slurry was filtered and the solid washed with isohexane (2 x 200 mL) then dried in a vacuum oven at 4O0C to leave a white solid (34.5 g). This solid was further dried in a vacuum oven at 6O0C overnight to leave the desired compound as a white powder (33.1 g). This is a different crystal form to that previously described, melting point (melting onset) 113.80C. The preparation of 2-(azetidin-l-ylcarbonyl)-5-chloropyrazine was described earlier. The preparation of 1,1-dimethylethyl 3-[({3-hydroxy-5-[(l- methylethyl)oxy]phenyl}carbonyl)amino]-lH-pyrazole-l-carboxylate is described below.
1 , 1 -Dimethylethyl 3-[(13 -hydroxy-5 - |Y 1 -methylethvDoxylphenyl } carbonyDamino] -IH- pyrazole- 1 -carboxylate
Figure imgf000178_0001
1 , 1 -Dimethylethyl 3-[({ 3-[( 1 -methylethyl)oxy]-5- [(phenylmethyl)oxy]phenyl}carbonyl)amino]-lH-pyrazole-l -carboxylate (76.4 g, 0.17 mol) was dissolved in ethanol (750 mL, 10 vol) and 10% palladium on charcoal (water wet) (7.5 g) added. The mixture was hydrogenated for 18 hours at 250C and a pressure of 5 bar. The catalyst was removed by filtration through celite ® and the cake washed with methanol (250 mL). The combined filtrate was concentrated under vacuum to leave a beige foamy solid which was chromatographed on silica, eluting with 10-70% ethyl acetate in isohexane, to give the desired material as a white foam solid (56.4 g). 1H NMR δ (CDCl3): 1.30 (d, 6H), 1.64 (s, 9H), 4.49 - 4.55 (m, IH), 6.67 (t, IH), 6.98 (d, 2H), 7.10 (d, IH), 8.02 (d, IH), 9.21 (s, IH); m/z 360 (M-H)"
1.1 -Dimethylethyl 3-f f {3-[Yl -methylethvnoxyl-5- [(phenylmethvDoxylphenvDcarbonvDaminoi-lH-pyrazole-l-carboxylate
Figure imgf000178_0002
A solution of oxalyl chloride (76 mL, 0.87 mol) in DCM (125 mL) was added dropwise to a slurry of 3-[(l-methylethyl)oxy]-5-[(phenylmethyl)oxy]benzoic acid (50 g, 0.17 mol) and DMF (1 mL) in DCM (300 mL). The reaction was stirred at RT for 2 hours then the solvent removed under vacuum and the residue azeotroped with toluene (200 mL). The oily residue was dissolved in dry pyridine (100 mL) and the mixture added, over 5 minutes, to a solution of 1,1-dimethylethyl 3-amino-lH-pyrazole-l-carboxylate (38.4 g, 0.21 mol) in dry pyridine (325 mL) under nitrogen. The reaction was stirred at RT for 1 hour, the solvent removed under vacuum and the residue azeotroped with toluene. The residue was partitioned between DCM (500 mL) and water (500 mL) and the organic layer washed with a saturated aqueous solution of sodium hydrogen carbonate (500 mL), brine (500 mL) and dried (MgSO4). The organic solution was concentrated under vacuum and the residue twice azeotroped with toluene to leave an orange resin (88.8 g). The resin was chromatographed on silica, eluting with 25-50% ethyl acetate in isohexane, to give the desired material as a pale yellow foam (76.4 g). 1H NMR δ (CDCl3): 1.33 (d, 6H), 1.56 (s, 9H), 4.49 - 4.55 (m, IH), 5.04 (s, 2H), 6.69 (t, IH), 7.00 (t, IH), 7.06 - 7.07 (m, IH), 7.17 (d, IH), 7.32 - 7.45 (m, 5H), 8.04 (d, IH), 9.48 (s, IH); m/z 450 (M-H)'
The preparations of 3-[(l-methylethyl)oxy]-5-[(phenylmethyl)oxy]benzoic acid and 1,1- dimethylethyl 3 -amino- lH-pyrazole- 1 -carboxylate were described earlier.
Example 40: S-lfS-fAzetidin-l-ylcarbonvD-S-chloropyridin^-ylloxyl-N-d^S- dimethyl-lH-pyrazol-3-yl)-5-{f(lS)-l-methyl-2-(methvIoxy)ethylloxy}benzamide
Figure imgf000179_0001
A solution of N-(l,5-dimethyl-lH-pyrazol-3-yl)-3-hydroxy-5-{[(15)-l-methyl-2-
(methyloxy)ethyl]oxy}benzamide (0.62 mmol) in acetonitrile (5 mL), was treated with potassium carbonate (1.24 mmol) and 5-(azetidin-l-ylcarbonyl)-2,3-dichloropyridine (0.68 mmol), before being heated to 12O0C in a microwave reactor for 4.5 hours. The mixture was evaporated in vacuo, before being partitioned between DCM (20 mL) and water (20 mL). The organics were evaporated in vacuo then chromatographed on silica, eluting with 0-10% methanol in ethyl acetate, to give the desired compound as a white foam (160 mg). 1H NMR δ (de-DMSO): 1.25 (d, 3H), 2.24 (s, 3H), 2.26 (m, 2H), 3.30 (s, 3H), 3.50 (m, 2H), 3.66 (s, 3H), 4.05 (t, 2H), 4.37 (t, 2H), 4.78 (m, IH), 6.42 (s, IH), 7.03 (t, IH), 7.38 (t, IH), 7.51 (t, IH), 8.23 (d, IH), 8.34 (d, IH), 10.76 (s, IH); m/z 514,516 (M+H)+
The preparation of 5-(azetidin-l-ylcarbonyl)-2,3-dichloropyridine was described earlier. The preparation of N-(l,5-dimethyl-lH-pyrazol-3-yl)-3-hydroxy-5-{[(liS)-l-metliyl-2- (methyloxy)ethyl]oxy}benzamide is described below:
N-(L5-Dimethyl-lH-pyrazol-3-ylV3-hvdroxy-5-{rri^-l-methyl-2- fmethyloxy)ethyll oxy Ibenzamide
Figure imgf000180_0001
A solution of N-(l,5-dimethyl-lH-pyrazol-3-yl)-3-{[(15)-l-methyl-2- (methyloxy)ethyl]oxy}-5-[(phenylmethyl)oxy]benzamide (2.57 mmol) in ethanol (40 mL), under argon was treated with 10% palladium on carbon (100 mg, wetted with 0.1 mL water and 1 mL ethanol). The flask was evacuated and filled with hydrogen, this procedure was repeated 4 times. The mixture was left to stir at RT overnight under an atmosphere of hydrogen then the reaction filtered through celite® and the cake washed with ethanol (50 mL) and ethyl acetate (50 mL). The combined organic layers were evaporated in vacuo to give the desired compound as a white foam (597 mg). 1H ΝMR δ (d6-DMSO): 1.23 (d, 3H), 2.25 (s, 3H), 3.31 (s, 3H), 3.47 (m, 2H), 3.66 (s, 3H), 4.66 (m, IH), 6.40 (s, IH), 6.46 (t, IH), 6.95 (t, IH), 7.06 (t, IH), 9.60 (s, IH)5 10.49 (s, IH); m/z 320 (M+H)+
N-ri.S-Dimethyl-lH-pyrazol-S-ylVS-irα^-l-methyl^-rmethyloxy ethylioxyl-S- rCphenylmethvDoxylbenzamide
Figure imgf000181_0001
A solution of 3-[(liS)-2-methoxy-(l-methylethyl)oxy]-5-{[phenylmethyl]oxy}benzoic acid (4.11 mmol) in DMF (10 mL) was treated with the l,5-dimethyl-lH-pyrazol-3-ylamine (4.52 mmol), DIPEA (5.93 mmol) and ΗATU (3.85 mmol) and stirred at RT overnight, under an argon atmosphere. The mixture was poured onto water (100 mL) and extracted with ethyl acetate (3 x 100 mL). The organic layer was washed with brine (20 mL) and evaporated in vacuo before chromatography on silica, eluting with 20-80% ethyl acetate in isohexane, to afford the desired compound as a yellow oil (1.05 g).
1H ΝMR δ (d6-DMSO): 1.23 (d, 3H), 2.25 (s, 3H), 3.30 (s, 3H), 3.48 (m, 2H), 3.67 (s, 3H), 4.71 (m, IH), 5.16 (s, 2H), 6.42 (s, IH), 6.73 (t, IH), 7.20 (t, IH), 7.26 (t, IH), 7.34 (m, IH), 7.41 (m, 2H), 7.46 (m, 2H)3 10.62 (s, IH); m/z410 (M+H)+
The preparation of 3-[(15)-2-methoxy-(l-methylethyl)oxy]-5-{[phenylmethyl]oxy}benzoic acid was described earlier.
The l,5-dimethyl-lH-pyrazol-3-ylamine was prepared according to the literature route [J. Het. Chem., (1982), 19, 1267].
Example 41; S-frS-fAzetidin-l-ylcarbonvD-S-chloropyridin-I-ylloxyl-iV-d^S- dimethyl-ljy-pyrazol-S-vD-S-lfl-fluoro-l-fflttoromethvDethylloxylbenzamide
Figure imgf000181_0002
A solution of N-(l,5-dimethyl-lH-pyrazol-3-yl)-3-{[2-fluoro-l-(fluoromethyl)ethyl]oxy}- 5-hydroxybenzamide (0.61 mmol) in acetonitrile (5 mL), was treated with the potassium carbonate (1.23 mmol) and 5-(azetidin-l-ylcarbonyl)-2,3-dichloropyridine (0.68 mmol), before being heated to 12O0C in a microwave reactor for 4 hours. The mixture was filtered and evaporated in vacuo before being chromatographed on silica, eluting with 0-5% methanol in ethyl acetate, to give the desired compound as a white foam (175 mg). 1H ΝMR δ (de-DMSO): 2.27 (m, 5H), 3.66 (s, 3H), 4.06 (t, 2H), 4.37 (t, 2H), 4.65 (m, IH), 4.73 (m, IH), 4.77 (m, IH), 4.85 (m, IH), 5.10 (m, IH), 6.42 (s, IH), 7.16 (t, IH), 7.45 (t, IH), 7.60 (t, IH), 8.23 (d, IH), 8.35 (d, IH), 10.71 (s, IH) m/z 520 (M+H)+
The preparation of 5-(azetidin-l-ylcarbonyl)-2,3-dichloropyridine was described earlier. The preparation of N-( 1 ,5 -dimethyl- lH-pyrazol-3 -yl)-3 - { [2-fluoro- 1 - (fluoromethyl)ethyl]oxy}-5-hydroxybenzamide is described below:
N-C 1.5-Dimethyl- lH-pyrazol-3-ylV3 -( r2-fluoro- 1 -f fluoromethyriethylioxy) -5- hvdroxybenzamide
Figure imgf000182_0001
A solution of N-(l,5-dimethyl-lH-pyrazol-3-yl)-3-{[2-fluoro-l-(fluoromethyl)ethyl]oxy}- 5-[(phenylmethyl)oxy]benzamide (1.89 mmol) in ethanol (30 mL) was treated with 10% palladium on carbon (78 mg, wetted with 1 mL ethanol). The flask was evacuated and filled with hydrogen and this procedure repeated a further 4 times. The mixture was left to stir at RT overnight under an atmosphere of hydrogen then the mixture filtered through celite® and the cake washed with ethanol (50 mL) and ethyl acetate (50 mL). The combined organics were evaporated in vacuo to give the desired compound as a white foam (613 mg).
1H ΝMR δ (d6-DMSO): 2.52 (s, 3Η), 3.66 (s, 3H), 4.66 (m, 2H), 4.77 (m, 2H), 4.97 (m, IH), 6.41 (s, IH), 6.56 (t, IH), 7.02 (m, IH), 7.15 (t, IH), 10.52 (s, IH); m/z 326 (M+H)+ N-rL5-Dimethyl-lH-pyrazol-3-ylV3-(r2-fluoro-l-rfluoromethvDethvnoxy)-5- rfohenylmethvDoxylbenzamide
Figure imgf000183_0001
A solution of 3-{[2-fluoro-l-(fluoromethyl)ethyl]oxy}-5-[(phenylmethyl)oxy]benzoic acid (2.96 mmol) in DMF (5 mL) was treated with l,5-dimethyl-lH-pyrazol-3-ylamine (3.11 mmol), DIPEA (5.93 mmol) and ΗATU (3.85 mmol) and stirred at RT overnight. The mixture was poured into water (100 mL) and extracted with ethyl acetate (3 x 75 mL). The organics were washed with brine, dried (MgSO4) and evaporated in vacuo before chromatography on solica, eluting with 50-100% ethyl acetate in isohexane, to give the desired compound as a colourless glass (784 mg). 1H ΝMR δ (d6-DMSO): 2.81 (s, 3H), 3.67 (s, 3H), 4.66 (m, 2H), 4.78 (m, 2H), 5.04 (m, IH), 5.69 (s, 2H), 6.43 (s, IH), 6.85 (t, IH), 7.28 (m, IH), 7.32 (m, IH), 7.35 (m, IH), 7.41 (m, 2H), 7.47 (m, 2H), 10.64 (s, IH); m/z 416 (M+H)+
The preparations of 3-{ [2-fluoro-l-(fluoromethyl)ethyl]oxy}-5-
[(phenylmethyl)oxy]benzoic acid and l,5-dimethyl-lH-pyrazol-3-ylamine were described earlier.
Example 42; 3-f[6-(Azetidin-l-vIcarbonvI)pyridazin-3-ylloxy}-5-{f(lS)-l-methyl-2- (methyloxy)ethvnoxy|-iV-(l-methvI-lH-pyrazol-3-yl)benzamide
Figure imgf000183_0002
A mixture of 3-(azetidin-l-ylcarbonyl)-6-chloropyridazine (45 mg, 0.23 mmol), 3- hydroxy-5 - [( liS)-2-methoxy-( 1 -methylethyl)oxy] -N-( 1 -methyl- lH-pyrazol-3 -yl)benzamide (70 mg, 0.23 mmol) and potassium carbonate (63 mg, 0.46 mmol) in acetonitrile (3 mL) was stirred in a Smith Creator microwave at 1300C for 1 hour. The solid was filtered off and the solvent was removed in vacuo. The residue was purified by reverse phase preparative HPLC, eluting with 5 - 95% acetonitrile in water (+ 0.2% TFA), followed by passage through a Silicycle Si-Carbonate cartridge, eluting with methanol, to give the desired product as a colourless solid (97 mg). 1H NMR δ (CDCl3): 1.34 (3H, d), 1.66 (3H, s), 2.33 - 2.41 (2H, m), 3.40 (3H, s), 3.48 - 3.52 (IH, m), 3.56 - 3.60 (IH, m), 3.79 (3H, s), 4.27 (2H, d), 4.58 - 4.62 (IH, m), 4.76 (2H, t), 6.79 (IH, d), 6.97 (IH, t), 7.25 - 7.29 (3H, m), 7.35 - 7.36 (IH, m), 8.26 - 8.28 (IH, m), 8.49 (IH, s); m/z 467 (M+H)+.
The preparation of 3 -hydroxy-5- [( 1 iS)-2-methoxy-( 1 -methylethyl)oxy] -N-( 1 -methyl- IH- pyrazol-3-yl)benzamide was described earlier.
The preparation of 3-(azetidin-l-ylcarbonyl)-6-chloropyridazine is decribed below:
3-(Azetidin- 1 -ylcarbonvD-6-chloropyridazine
Figure imgf000184_0001
Phosphorus oxy chloride (50 mL) was added to 6-hydroxypyridazine-3-carboxylic acid (9.82 g, 70.05 mmol) and the resulting solution heated to reflux. After 45 mins the solution was allowed to cool and the excess of phosphorus oxychloride was removed under reduced pressure. TΗF (50 mL) was added to the black residue and the resulting solution cooled to 0°C. Triethylamine (9.77 mL, 70.05 mmol) and azetidine (4.0 g, 70.05 mmol) were added dropwise. The resulting mixture was allowed to warm to RT and stirred overnight. The volatiles were removed under reduced pressure and water (50 mL) added, then the residue was adjusted to pΗ8 using sodium hydroxide solution. The aqueous layer was extracted five times with ethyl acetate, the combined organics dried (MgSO4) and the solvent removed to give the crude product as a dark oil. A sample was purified by preparative HPLC, eluting with 5 - 95% acetoniltrile in water (+ 0.2% TFA), and gave the title compound as a colorless solid (135 mg). 1H NMR δ (CDCl3): 2.39 - 2.47 (2H, m), 4.30 (2H, t), 4.82 (2H, t), 7.64 (IH, d), 8.21 (IH, d); m/z l98 (M+H)+.
Example 43: 3-{r5-(Azetϊdin-l-ylcarbonvπpyrazin-2-ylloxy}-iV-(4-methvI-l,3-thiazol- 2-vI)-5-f(3iy)-tetrahvdrofuran-3-yloxy1benzamide
Figure imgf000185_0001
3-{[5-(Azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-[(35)-tetraliydrofuran-3-yloxy]benzoic acid (200 mg, 0.52 mmol), HATU (475 mg, 1.25 mmol) and 2-amino-4-methylthiazole (119 mg, 1.04 mmol) were suspended in DMF (2 mL). DIPEA (0.220 mL, 1.26 mmol) was then added and the reaction mixture stirred at RT for 16 hours. The DMF was removed in vacuo and water (15 mL) added. The mixture was extracted with ethyl acetate (3 x 20 mL) and the extracts combined, washed with water (15 mL), IN hydrochloric acid (10 mL), water (10 mL), a saturated aqueous solution of sodium bicarbonate (10 mL), brine (10 mL), dried (MgSO4), filtered, and evaporated in vacuo. The residue was chromatographed on silica, eluting with a gradient of 60-100% ethyl acetate in isohexane, to give the desired compound (70 mg).
1H NMR δ (CDCl3): 2.13 - 2.29 (m, 2H), 2.32 (s, 3H), 2.34 - 2.42 (m, 2H), 3.89 - 4.02 (m, 4H), 4.26 (t, 2H), 4.69 (t, 2H), 4.96 - 4.99 (m, IH), 6.57 (d, IH), 6.94 (t, IH), 7.29 (t, IH), 7.33 (t, IH), 8.35 (d, IH), 8.85 (d, IH), 9.72 (s, IH); m/z 482 (M+H)+, 480 (M-H)" The following compounds were prepared in an analogous fashion from 3-{[5-(azetidin-l- ylcarbonyl)pyrazin-2-yl]oxy}-5-[(3iS)-tetrahydrofuran-3-yloxy]benzoic acid and the appropriate aminoheterocycle. -
Figure imgf000186_0001
Figure imgf000187_0002
* The reaction was carried out using 1,1-dimethylethyl 3-amino-lH-pyrazole-l- carboxylate as the amino heterocycle, the resultant product was dissolved in acetonitrile and heated in a microwave reactor at 15O0C for 5 - 10 minutes. The mixtures were allowed to cool then concentrated in vacuo to give the desired products. The preparation of 1,1- dimethylethyl 3-amino-lH-pyrazole-l-carboxylate was described earlier. The preparation of 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-[(35)- tetrahydrofuran-3-yloxy]benzoic acid is described below:
3 - { [5-(Azetidin- 1 -ylcarbonvDpyrazin-2-yl'|oxy } -5 - [(3>SVtetrahvdrofuran-3 -yloxyibenzoic acid
Figure imgf000187_0001
Methyl 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-[(31S)-tetrahydrofuran-3- yloxy]benzoate (7.02 mmol) was dissolved in TΗF (50 mL), IN sodium hydroxide (7.0 mL) was added followed by water (50 mL) and the resultant solution stirred at RT for 4 hours. The organics were removed in vacuo, the aqueous solution filtered and extracted with ethyl acetate (30 mL). The aqueous layer was acidified with 2N hydrochloric acid, extracted with ethyl acetate (3 x 50 mL), and the organic extracts washed with water (10 mL), brine (10 mL) then evaporated to dryness to give the desired material as a white foam (2.33 g). 1H NMR δ (CDCl3): 2.14 - 2.30 (m, 2H), 2.34 - 2.42 (m, 2H), 3.89 - 3.94 (m, 2H), 3.97 - 4.02 (m, 2H), 4.28 (t, 2H), 4.69 (t, 2H), 4.97 - 5.00 (m, IH), 6.94 (t, IH), 7.48 (t, 2H), 8.34 (d, IH), 8.85 (d, IH); m/z 386 (M+H)+, 384 (M-H)" Methyl S-irS-razetidin-l-ylcarbonvDpyrazin^-ylloxyl-S-frS-S^-tetrahydrofuran-S- yloxy]benzoate
Figure imgf000188_0001
A solution of methyl 3-hydroxy-5-[(3S)-tetrahydrofuran-3-yloxy]benzoate (2.5 g, 10.5 mmol), potassium carbonate (2.9 g, 21.0 mmol) and 2-(azetidin-l-ylcarbonyl)-5- chloropyrazine (2.48 g, 12.6 mmol) in DMA (25 mL) was heated at 120°C for 2 hours. The solution was diluted with ethyl acetate (150 mL), washed with water (3 x 50 mL), brine (20 mL), dried (MgSO4), filtered and the solvent removed in vacuo. The residue was purified by chromatography on silica, eluting with 0-50% ethyl acetate in isohexane, to give the desired compound as a colourless oil (2.8 g). 1H NMR δ (CDCl3): 2.13 - 2.29 (m, 2H), 2.34 - 2.41 (m, 2H), 3.88 - 4.04 (m, 4H), 3.90 (s, 3H), 4.25 (t, 2H), 4.68 (t, 2H), 4.96 - 5.00 (m, IH), 6.91 (t, IH), 7.43 (d, 2H), 8.32 (d, IH), 8.85 (d, IH); m/z 386 (M+H)+, 384 (M.-ΕS)'
Methyl 3-hvdroxy-5-[f3iSVtetrahvdrofuran-3-yloxy1benzoate
Figure imgf000188_0002
Methyl 3-[(phenylmethyl)oxy]-5-[(3)S)-tetrahydrofuran-3-yloxy]benzoate (25.0 g, 76.2 mmol) was dissolved in THF (150 mL) and ethanol (150 mL). 10% Palladium on carbon (30 mg) was added and the mixture placed under a hydrogen atmosphere and left to stir at RT until the reaction was complete. The catalyst was removed by filtration through diatomaceous earth and the filtrate was concentrated in vacuo to give an orange oil which crystallised on standing. The solid was filtered off and washed with diethyl ether to give the desired product as a white solid (13.75 g).
1H NMR δ (CDCl3): 2.1-2.3 (2H, m), 3.9 (3H, s), 3.9-3.95 (2H, m), 3.97-4.05 (2H, m), 4.95 (IH, s), 5.6 (1), 6.6 (IH, t), 7.1 (IH, t), 7.13 (IH, t); m/z 237 (M+H)+ Methyl 3-[(phenylmethyl')oxyl-5-[(3»Sf)-tetrahydrofuran-3-yloxy]benzoate
Figure imgf000189_0001
A mixture of methyl 3-hydroxy-5-{[phenylmethyl]oxy}benzoate (18.8 g, 72.75 mmol), (3i?)-tetrahydrofuran-3-yl 4-methylbenzenesulfonate (18.5 g, 76.4 mmol) and potassium carbonate (20.08 g, 145.5 mmol) in butyronitrile (250 mL) was heated to 130°C for 3 hours. The solvent was removed in vacuo and ethyl acetate added. The organics were washed with water (40 mL), 0.5M sodium hydroxide solution (40 mL), brine (40 mL), dried (MgSO4), filtered and the solvent removed in vacuo. The residue was chromatographed on silica, eluting with a gradient of 0-5% methanol in DCM, to give the desired compound as a colourless oil (20.1 g). 1H NMR δ (CDCl3): 2.08 - 2.26 (m, 2H), 3.78 - 4.01 (m, 4H), 3.90 (s, 3H), 4.92 - 4.96 (m, IH), 5.08 (s, 2H), 6.69 (t, IH), 7.15 (t, IH), 7.29 (t, IH), 7.34 - 7.44 (m, 5H); m/z 327 (M+H)+
The preparations of methyl 3-hydroxy-5-{[phenylmethyl]oxy}benzoate and (3R)- tetrahydrofuran-3-yl 4-methylbenzenesulfonate were described earlier.
Example 44 : 3-{ f 5-(Azetidin-l-ylcarbonvD-3-chloropyridin-2-vIl oxyl-7V-(4-methyl- l,3-thiazol-2-yl)-5-f(3S)-tetrahvdrofuran-3-yloxylbenzamide
Figure imgf000189_0002
l-Chloro-N,N,2-trimethyl-l-propenylamine (0.70 mL, 0.53 mmol) was added to a solution of 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-[(36)-tetrahydrofuran-3- yloxy]benzoic acid (200 mg, 0.48 mmol) in DCM (6 mL) and stirred at RT for 2 hours. 2- Amino-4-methylthiazole (110 mg, 0.96 mmol) and pyridine (0.078 mL, 0.96 mmol) were added and the reaction stirred at RT for 16 hours. The solvent was removed in vacuo, water (20 mL) added and the mixture extracted with ethyl acetate (3 x 20 mL). The extracts were combined and washed with 2N hydrochloric acid (20 mL), saturated aqueous sodium bicarbonate solution (20 mL), water (20 mL), brine (20 mL), dried (MgSO4), filtered, and evaporated in vacuo. The residue was chromatographed on silica, eluting with a gradient of 50-100% ethyl acetate in isohexane, to give the desired compound (78 mg). 1H NMR δ (CDCl3): 2.13 - 2.28 (m, 2H), 2.31 (s, 3H), 2.35 - 2.43 (m, 2H), 3.88 - 4.02 (m, 4H), 4.21 - 4.28 (m, 2H), 4.31 - 4.39 (m, 2H), 4.96 - 5.00 (m, IH), 6.56 (d, IH), 6.94 (t, IH), 7.28 (t, IH), 7.34 (t, IH), 8.16 (d, IH), 8.25 (d, IH), 9.83 (s, IH); m/z 515 (M+H)+, 513 (M-H)-
The following compounds were prepared in an analogous fashion from 3-{[5-(azetidin-l- ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-[(35)-tetrahydrofuran-3-yloxy]benzoic acid and the appropriate aminoheterocycle.
Figure imgf000190_0001
Figure imgf000191_0001
* The reaction was carried out using 1,1-dimethylethyl 3-amino-lH-pyrazole-l- carboxylate as the amino heterocycle, the resultant product was dissolved in acetonitrile and heated in a microwave reactor at 15O0C for 5 - 10 minutes. The mixtures were allowed to cool then concentrated in vacuo to give the desired products. The preparation of 1,1- dimethylethyl 3-amino-lH-pyrazole-l-carboxylate was described earlier. The preparation of 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-[(35)- tetrahydrofuran-3-yloxy]benzoic acid is described below: 3-{[5-(Azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl1oxy}-5-r('3^-tetrahvdrofuran-3- yloxylbenzoic acid
Figure imgf000192_0001
Methyl 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-[(3iS)-tetxahydrofuran- 3-yloxy]benzoate (3.75 g, 8.68 mmol) was dissolved in THF (40 mL), IN sodium hydroxide (8.68 mL, 8.68 mmol) was added followed by water (40 mL) and methanol (1 drop) and the resultant solution stirred at RT for 3 hours. The organics were removed in vacuo, the aqueous solution filtered and extracted with ethyl acetate (30 mL). The aqueous layer was acidified with 2N hydrochloric acid, extracted with ethyl acetate (3 x 50 mL), and the organic extracts washed with water (10 mL), brine (10 mL) then evaporated to dryness to give the desired material as a white foam (3.53 g).
1R NMR δ (CDCl3): 2.14 - 2.30 (m, 2H), 2.35 - 2.43 (m, 2H), 3.89 - 3.94 (m, 2H), 3.96 - 4.02 (m, 2H), 4.22 - 4.38 (m, 4H), 4.97 - 5.01 (m, IH), 6.95 (t, IH), 7.46 - 7.47 (m, IH), 7.49 - 7.50 (m, IH), 8.15 (d, IH), 8.26 (d, IH); m/z 419 (M+H)+, 417 (M-H)".
Methyl 3-{[5-razetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-r(3)S^-tetrahydrofuran- 3 - yloxylbenzoate
Figure imgf000192_0002
A solution of methyl 3-hydroxy-5-[(35)-tetrahydrofuran-3-yloxy]benzoate (2.5 g, 10.5 mmol), potassium carbonate (2.9 g, 21.0 mmol) and 5-(azetidin-l-ylcarbonyl)-2,3- dichloropyridine (2.9 g, 12.6 mmol) in DMA (25 mL) was heated at 120°C for 5 hours. The solution was diluted with ethyl acetate (150 mL), washed with water (3 x 50 mL), brine (20 mL), dried (MgSO4), filtered and the solvent removed in vacuo. The residue was purified by chromatography on silica, eluting with 20-50% ethyl acetate in isohexane, to give the desired compound as a colourless oil (3.75 g). 1H NMR δ (CDCl3): 2.13 - 2.29 (m, 2H), 2.38 (quin, 2H), 3.85 - 4.04 (m, 4H), 3.90 (s, 3H), 4.19 - 4.37 (m, 4H), 4.96 - 5.00 (m, IH), 6.91 (t, IH), 7.42 - 7.45 (m, 2H), 8.14 (d, IH), 8.25 (d, IH); m/z 433 (M+H)+.
The preparations of methyl 3-hydroxy-5-[(3iS)-tetrahydrofuran-3-yloxy]benzoate and 5- (azetidin-l-ylcarbonyl)-2,3-dichloropyridine were described earlier.
Example 45; S-irό-fAzetidin-l-ylcarbonvDpyridin-S-vIloxyl-iV-fS-methvIpyrazin-l- yl)-5-[(3S)-tetrahvdrofuran-3-yloxy1benzamide
Figure imgf000193_0001
l-Chloro-N,N,2-trimethyl-l-propenylamine (0.133 mL, 1.0 mmol) was added to a suspension of 3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3 -yl]oxy } -5- [(3<S)-tetrahydrofuran-3 - yloxy]benzoic acid (350 mg, 0.94 mmol) in DCM (10 mL) and stirred at RT for 30min. 2- Amino-5-methylpyrazine (196 mg, 1.82 mmol) and pyridine (0.148 mL, 1.82 mmol) were added and the reaction stirred at RT for 2 hours. The solvent was removed in vacuo and water (30 mL) added. The mixture was extracted with ethyl acetate (3 x 20 mL), washed with 2Ν hydrochloric acid (20 mL), water (10 mL), saturated aqueous sodium bicarbonate solution (20 mL), water (10 mL) and brine (20 mL). The combined organic extracts were dried (MgSO4), filtered and evaporated in vacuo. The residue was purified by chromatography, eluting with 0-10% methanol in DCM, to give the desired product as a colourless oil (150 mg). 1H NMR δ (CDCl3): 2.12 - 2.18 (IH, m), 2.22 - 2.29 (IH, m), 2.31 - 2.40 (2H, m), 2.56 (3H, s), 3.89 - 4.03 (4H, m), 4.25 (2H, t), 4.71 (2H, t), 4.97 - 5.00 (IH, m), 6.76 (IH, t), 7.15 (IH, t), 7.25 (IH, d), 7.38 - 7.40 (IH, m), 8.12 (IH, s), 8.14 (IH, d), 8.32 (IH, s), 8.34 (IH, d), 9.52 (IH, d); m/z 476 (M+H)+, 474 (M-H)-
The following compound was prepared in an analogous fashion from 3-{[5-(azetidin-l- ylcarbonyl)pyrazin-2-yl]oxy}-5-[(35)-tetrahydrofuran-3-yloxy]benzoic acid or 3-{[6- (azetidin- 1 -ylcarbonyl)pyridin-3-yl]oxy}-5-[(3iS)-tetxahydrofuran-3-yloxy]benzoic acid and the appropriate aminoheterocycle.
2.34 - 4H), IH), (s,
2.32 - 2H), IH), - -
2.32 - 2H), IH),
2.35 - (t, 2H), IH), - IH),
2.35 - (t, 2H), IH), IH), IH),
Figure imgf000194_0001
The preparation of 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-[(3»S)- tetrahydrofuran-3-yloxy]benzoic acid was described earlier. The preparation of 3-{ [6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-[(35)- tetrahydrofuran-3-yloxy]benzoic acid is described below:
S-irό-fAzetidin-l-ylcarbonyπpyridin-S-ynoxyl-S-rG^-tetrahvdrofuran-S-yloxylbenzoic acid
Figure imgf000195_0001
Methyl 3-hydroxy-5-[(35)-tetrahydrofuran-3-yloxy]benzoate (875 mg, 3.68 mmol), 2- (azetidin-l-ylcarbonyl)-5-bromopyridine (1.06 g, 4.41 mmol ), caesium carbonate (3.59 g, 11.03 mmol), and bromotris(triphenylphosphine)copper (0.69 g, 0.735 mmol) were added together in DMA (16 mL) and heated in a microwave reactor at 16O0C for 9 hours. The mixture was taken up in water (25 mL) and washed with ethyl acetate (2 x 20 mL). The aqueous layer was acidified with IN hydrochloric acid (30 mL) and extracted with ethyl acetate (5 x 30 mL). The combined organic extract was washed with water (30 mL), brine (30 mL), dried (MgSO4), filtered and evaporated to give a brown solid which was triturated with diethyl ether to give the desired compound as a beige solid (820 mg). 1H NMR δ (d6-DMSO): 2.00 - 2.06 (m, IH), 2.23 - 2.37 (m, 3H), 3.78 - 3.95 (m, 4H), 4.13 (t, 2H), 4.64 (t, 2H), 5.16 - 5.19 (m, IH), 7.05 (t, IH), 7.18 - 7.19 (m, IH), 7.33 - 7.34 (m, IH), 7.62 (dd, IH), 8.04 (d, IH), 8.46 (d, IH); m/z 385 (M+H)+, 383 (M-H)"
The preparations of methyl 3-hydroxy-5-[(3iS)-tetrahydrofuran-3-yloxy]benzoate and 2- (azetidin-l-ylcarbonyl)-5-bromopyridine were described earlier. Example 46: 3-{r3-Chloro-5-(pyrrolidin-l-ylcarbonyr)pyridin-2-ylloxy}-5--[(l- methyIethyl)oxyl-iV-(5-methylpyrazin-2-yl)benzainide
Figure imgf000196_0001
Cesium carbonate (782 mg, 2.4 mmol) was added to a solution of 3-hydroxy-5-[(l- methylethyl)oxy]-N-(5-methylpyrazin-2-yl)benzamide (230 mg, 0.8 mmol) and 2,3- dichloro-5-(pyrrolidin-l-ylcarbonyl)pyridine (234 mg, 0.96 mmol) in acetonitrile (5 mL) and the stirred mixture heated in a microwave reactor at 12O0C for 2 hours. The mixture was cooled to RT and the acetonitrile evaporated in vacuo. The residue was partitioned between water (25 mL) and ethyl acetate (50 mL), the organic layer washed with brine, dried (MgSO4) and evaporated to a residue which was chromatographed on silica, eluting with 75% ethyl acetate in isohexane, to give the desired compound (290 mg). 1H ΝMR δ (CDCl3): 1.3 (d, 6H), 1.9 (m, 4H), 2.5 (s, 3H), 3.4-3.7 (m, 4H), 4.55 (m, IH), 6.85 (d, IH), 7.2 (d, IH), 7.25 (d, IH), 7.9 (d, IH), 8.1 (dd, IH), 8.2 (s, IH), 8.35 (s, IH) and 9.45 (s, IH); m/z 496 (M+H)+. The following compounds were prepared in an analogous fashion from 2,3-dichloro-5- (pyrrolidin-l-ylcarbonyl)pyridine and either 3-hydroxy-5-{[(15)-l-methyl-2- (methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide or 3-hydroxy-N-(l-methyl- lH-pyrazol-3-yl)-5-[(35)-tetrahydrofuran-3-yloxy]benzamide.
Figure imgf000196_0002
46b 512 IH NMR δ (CDCl3): 1.8 - 1.95 (m, 4H), 2.1 (M+H)+ (m, 2H), 3.45 (t, 2H), 3.6 (t, 2H), 3.7 (s, 3H), 3.85 (m, 2H), 3.95 (d, 2H), 4.9 (m, IH), 6.7 (d, IH), 6.8 (d, IH), 7.15 (d, IH), 7.25 (d, 2H),
Figure imgf000197_0001
7.95 (d, IH), 8.1 (d, IH), 8.9 (s, IH)
The preparation of 2,3-dichloro-5-(pyrrolidin-l-ylcarbonyl)pyridine is described below:
2,3-Dichloro-5-(pyrrolidin-l-ylcarbonyl)pyridine
Figure imgf000197_0002
Oxalyl chloride (3.2 mL; 36.0 mmol) was added to a solution of 5,6 dichloronicotinic acid (5.76 g; 30.0 mmol) and 4M hydrogen chloride in dioxan (7.5 mL; 30.0 mmol) in DCM (50 mL). The mixture was stirred at RT for 18 hours, the DCM evaporated in vacuo, the residue azeotroped with toluene (2 x 15 mL) then added to a solution of pyrrolidine (3.0 mL; 36.0 mmol) and triethylamine (10.0 mL; 72 mmol) in DCM (150 mL). The mixture was stirred at RT for 6 hours, the DCM evaporated in vacuo, the residue partitioned between water (75 mL) and ethyl acetate (100 mL), the organic layer washed with brine, dried (MgSO4) and evaporated in vacuo. The residue was chromatographed on silica, eluting with 50% ethyl acetate in isohexane, to give the desired compound which was further purified by crystallisation from ethyl acetate and isohexane (6.88 g). 1H NMR δ (CDCl3): 1.9 (m, 4H), 3.4 (m, 2H), 3.6 (s, 3H), 7.9 (s, IH) and 8.4 (s, IH); m/z 245 (M+H)+.
The preparations of 3-hydroxy-5-[(l-methylethyl)oxy]-iV-(5-methylpyrazin-2- yl)benzamide and 3-hydroxy-5-{[(15)-l-methyl-2-(methyloxy)ethyl]oxy}-N-(5- methylpyrazin-2-yl)benzamide were described earlier. Example 47 ; 3-{ [5-(Azetidin-l-ylcarbonyl)pyrazin-2-yll oxyl-iV-(l-methyl-lH-pyrazol- 3-yl)-5-(tetrahvdro-2H-pyran-4-vIoxy)benzamide
Figure imgf000198_0001
3 -Ηydroxy-N-( 1 -methyl- lH-pyrazol-3 -yl)-5 -(tetrahydro-2H-pyran-4-yloxy)benzamide (209 mg, 0.66 mmol), 2-(azetidin-l-ylcarbonyl)-5-chloropyrazine (159 nig, 0.80 mmol) and potassium carbonate (184 mg, 1.33 mmol) were dissolved / suspended in acetonitrile (3.5 niL). The reaction mixture was heated in a microwave reactor for 4 hours at 12O0C then cooled, filtered and reduced in vacuo. The crude product was purified by chromatography on silica, eluting with 0-5% methanol in DCM, to give the required product as a white foam (39 mg). 1H ΝMR δ (d6-DMSO): 1.57 - 1.68 (m, 2H), 1.97 - 2.05 (m, 2H), 2.26 - 2.36 (m, 2H), 3.47 - 3.55 (m, 2H), 3.78 (s, 3H), 3.84 - 3.91 (m, 2H), 4.10 (t, 2H), 4.57 (t, 2H), 4.67 - 4.75 (m, IH), 6.58 (d, IH), 7.11 (t, IH), 7.43 (t, IH), 7.54 (t, IH), 7.60 (d, IH), 8.56 (d, IH), 8.68 (d, IH), 10.80 (s, IH); m/z 479 (M+H)+
The following compounds were made in an analogous fashion from the either 3-hydroxy- N-(I -methyl- lH-pyrazol-3 -yl)-5 -(tetrahydro-2H-pyran-4-yloxy)benzamide or 3 -hydroxy- N-(5-methylpyrazin-2-yl)-5-(tetrahydro-2H-pyran-4-yloxy)benzamide and the appropriate halogenated heterocycle.
Figure imgf000198_0002
Figure imgf000199_0002
The preparations of 2-(azetidin-l-ylcarbonyl)-5-chloropyrazine and 5-(azetidin-l- ylcarbonyl)-2,3-dichloropyridine were described earlier.
The preparation of 3 -hydroxy-N-( 1 -methyl- lH-pyrazol-3 -yl)-5 -(tetrahydro-2H-pyran-4- yloxy)benzamide is described below:
3 -Ηydroxy-N-C 1 -methyl- lH-pyrazol-3 -yP-5 -(tetrahvdro-2H-pyran-4-yloxy)benzamide
Figure imgf000199_0001
N-(I -Methyl- lH-pyrazol-3 -yl)-3 - [(phenylmethyl)oxy] -5 -(tetrahydro-2H-pyran-4- yloxy)benzamide (1.85 g, 4.54 mmol) was dissolved in ethanol (40 mL) and 10% palladium on charcoal (97 mg) catalyst added under argon. The reaction was stirred under an atmosphere of hydrogen for 16 hours then filtered through Celite® and the filtrate concentrated in vacuo give the desired compound as a light brown solid (1.30 g). 1H ΝMR δ (d6-DMSO): 1.54 - 1.65 (m, 2Η), 1.94 - 2.03 (m, 2H), 3.44 - 3.54 (m, 2H), 3.78 (s, 3H), 3.83 - 3.90 (m, 2H), 4.56 - 4.65 (m, IH), 6.51 (t, IH), 6.56 (d, IH), 6.97 (t, IH), 7.08 (t, IH), 7.59 (d, IH), 9.63 (s, IH), 10.59 (s, IH); m/z 318 (M+H)+ N-ri-Methyl-lH-pyrazol-3-ylV3-rrphenylmethyl)oxyl-5-rtetrahvdro-2H-pyran-4- yloxy)benzamide
Figure imgf000200_0001
3-[(Phenylmethyl)oxy]-5-(tetrahydro-2H-pyran-4-yloxy)benzoic acid (3.14 g, 9.5 mmol), 1 -methyl- lH-pyrazol-3-amine (1.85 g, 19.0 mmol) and ΗATU (4.70 g, 12.35 mmol) were dissolved in DMF (12.5 mL) and DIPEA (3.31 mL, 19.0 mmol) added. The resultant mixture was stirred at RT for 20 hours. The mixture was quenched with water (150 mL), extracted with ethyl acetate (2 x 75 mL), washed with brine, dried (MgSO4) and concentrated in vacuo. The residue was chromatographed on silica, eluting with 0-50% ethyl acetate in isohexane, to give the desired compound as a pale yellow gum (1.85 g).
3 - lYPhenyrmethvDoxyl -5 -(tetrahvdro-2H-pyran-4-yloxy)benzoic acid
Figure imgf000200_0002
Methyl 3-[(phenylmethyl)oxy]-5-(tetrahydro-2H-pyran-4-yloxy)benzoate (9.00 g, 26.3 mmol) was dissolved in TΗF (120 mL) and methanol (30 mL) added a solution of lithium hydroxide monohydrate (3.24 g, 78.9 mmol) in water (60 mL). The bi-phasic solution was stirred at RT for 3 hours until complete conversion was indicated by LCMS. The organics were removed in vacuo and water (40 mL) added. The pΗ of the mixture was adjusted to 7 by the addition of hydrochloric acid and the resulting precipitate filtered and washed with cold water to give the desired compound as a white solid (8.03 g). 1H ΝMR δ (dβ-DMSO): 1.57 (m, 2H), 1.94 (m, 2H), 3.50 (m, 2H), 3.84 (m, 2H), 4.62 (m, IH), 5.15 (s, 2H), 6.87 (t, IH), 7.09 (m, IH), 7.14 (m, IH), 7.34 (t, IH), 7.40 (t, 2H), 7.46 (d, 2H), 12.99 (s, IH); m/z 327 (M+H)+ Methyl 3-[CphenylmethyDoxy1-5-(tetrahydro-2H-pyran-4-yloxy')benzoate
Figure imgf000201_0001
Methyl 3-hydroxy-5-{[phenylmethyl]oxy}benzoate (7.75 g, 30.0 mmol), tetrahydro-4H- pyra-4-ol (4.28 mL, 45.0 mmol) and triphenylphosphine (11.8 g, 45.0 mmol) were stirred under argon in TΗF (166 mL) and cooled to 50C in an ice bath. DEAD (19.6 mL, 45.0 mmol) was added dropwise to the mixture, maintaining the internal temperature below 1O0C. Stirring was continued for 16 hours then the reaction mixture reduced in vacuo and the residue redissolved in ethyl acetate (60 mL) and isohexane (60 mL). The precipitate was removed, the filtrate concentrated in vacuo and purified by chromatography on silica, eluting with 0-25% ethyl acetate in isohexane (a small quantity of DCM used to assist column loading), to give the desired compound as a colourless oil (9.0 g). 1H NMR δ (de-DMSO): 1.58 (m, 2H), 1.94 (m, 2H), 3.49 (m, 2H)3 3.84 (m, 5H), 4.65 (m, IH), 5.16 (s, 2H), 6.92 (t, IH), 7.10 (m, IH), 7.15 (m, IH), 7.34 (t, IH), 7.40 (t, 2H), 7.46 (d, 2H); m/z 343 (M+H)+
The preparation of methyl 3-hydroxy-5-{[phenylmethyl]oxy}benzoate was described earlier. The preparation of 3-hydroxy-N-(5-methylpyrazin-2-yl)-5-(tetrahydro-2H-pyran-4- yloxy)benzamide is described below:
3-Ηvdroxy-N-(5-methylpyrazin-2-yl)-5-rtetrahydro-2H-pyran-4-yloxy)benzamide
Figure imgf000201_0002
N-(5-Methylpyrazin-2-yl)-3-[(phenylmethyl)oxy]-5-(tetrahydro-2H-pyran-4- yloxy)benzamide (1.70 g, 4.05 mmol) was dissolved in ethanol (40 mL) and 10% palladium on charcoal (86 mg) catalyst added under argon. The reaction was stirred under an atmosphere of hydrogen for 4 days then filtered and the filtrate concentrated in vacuo to give the desired compound as a cream-coloured solid (1.20 g). m/z 330 (M+H)+
N-(5-Methylpyrazin-2-ylV3-r(phenylmethvDoxyl-5-(tetrahvdro-2H-pyran-4- yloxy)benzamide
Figure imgf000202_0001
3-[(Phenylmethyl)oxy]-5-(tetrahydro-2H-pyran-4-yloxy)benzoic acid (3.14 g, 9.5 mmol), 2-amino-5 methylpyrazine (2.08 g, 19.0 mmol) and ΗATU (4.70 g, 12.35 mmol) were dissolved in DMF (12.5 niL) and DIPEA (3.31 mL, 19.0 mmol) added. The resultant mixture was stirred at RT for 20 hours. The mixture was quenched with water (150 mL) and extracted with ethyl acetate (2 x 75 mL), washed with brine, dried (MgSO4) and concentrated in vacuo. The residue was chromatographed on silica, eluting with 0-50% ethyl acetate in isohexane, to give the desired compound as a pale yellow gum (1.70 g). m/z 420 (M+Η)+
The preparations of 3-[(phenylmethyl)oxy]-5-(tetrahydro-2H-pyran-4-yloxy)benzoic acid and 2-amino-5 methylpyrazine were described earlier.
Example 48; 3-{f6-(Azetidin-l-ylcarbonyl)pyridin-3-vnoxy}-iV-(l-methvI-lH-pyrazol- 3-yl)-5-(tetrahvdro-2H-pyran-4-yloxy)benzamide
Figure imgf000202_0002
3-Ηydroxy-N-(l-methyl-lH-pyrazol-3-yl)-5-(tetrahydro-2H-pyran-4-yloxy)benzamide (200 mg, 0.63 mmol), 2-(azetidin-l-ylcarbonyl)-5-bromopyridine (184 mg, 0.76 mmol), bromotris(triphenylphosphine)copper (186 mg, 0.2 mmol) and caesium carbonate (411 mg, 1.26 mmol) were dissolved / suspended in DMA (3.5 mL). The reaction mixture was heated in a microwave reactor for 1 hour at 2000C then allowed to cool, filtered and reduced in vacuo. Water (25 mL) was added and the product extracted with ethyl acetate (2 x 25 mL), the organics dried (MgSO4) then purified by chromatography on silica, eluting with 0-10% methanol in DCM, to give the desired compound as a pale yellow foam (65 mg). 1R NMR δ (de-DMSO): 1.57 - 1.67 (m, 2H), 1.97 - 2.05 (m, 2H), 2.23 - 2.34 (m, 2H), 3.51 (t, 2H), 3.78 (s, 3H), 3.83 - 3.90 (m, 2H), 4.08 (t, 2H), 4.59 (t, 2H), 4.69 - 4.76 (m, IH), 6.56 (d, IH), 7.00 (t, IH), 7.29 (t, IH), 7.49 (t, IH), 7.54 - 7.58 (m, IH), 7.60 (d, IH), 8.00 (d, IH), 8.41 (d, IH), 10.83 (s, IH); m/z 478 (M+H)+
The folowing compound was made in an analogous fashion from 2-(azetidin-l- ylcarbonyl)-5-bromopyridine and 3-hydroxy-iV-(5-methylpyrazin-2-yl)-5-(tetrahydro-2H- pyran-4-yloxy)benzamide .
Figure imgf000203_0001
The preparations of 2-(azetidin-l-ylcarbonyl)-5-bromopyridine, 3-hydroxy-N-(l-methyl- lH-pyrazol-3-yl)-5-(tetrahydro-2H-pyran-4-yloxy)benzamide and 3-hydroxy-N-(5- methylpyrazin-2-yl)-5-(tetrahydro-2H-pyran-4-yloxy)benzamide were described earlier. Example 49: S-lfβ-fAzetidin-l-ylcarbonvDpyridin-B-vnoxyl-JV-d-methyl-lH-pyrazol- 3-yl)-5-f(3R*)-tetrahydrofuran-3-yIoxylbenzamide
Figure imgf000204_0001
A mixture of 3-hydroxy-N-(l -methyl- lH-pyrazol-3-yl)-5-[(3i?)-tetrahydrofuran-3- yloxy]benzamide (150 mg, 0.49 mmol), 2-(azetidin-l-ylcarbonyl)-5-bromopyridine (120 mg, 0.49 mmol), bromotris(triphenylphosphine)copper (92 mg, 0.10 mmol) and caesium carbonate (484 mg, 1.48 mmol) in acetonitrile (7 mL) was stirred in a microwave reactor at 1600C for 6 hours. The solid was filtered off, the solvent was removed in vacuo and ethyl acetate (50 mL) added to the residue. The organic phase was washed with water (10 mL), brine (20 mL), dried (MgSO4), filtered and the solvent removed in vacuo. The residue was chromatographed on silica, eluting with a gradient of 0-10% methanol in DCM, to give the title compound as a white solid (86 mg). 1H ΝMR δ (CDCl3): 2.10 - 2.17 (m, IH), 2.19 - 2.25 (m, IH)52.32 - 2.38 (m, 2H), 3.76 (s, 3H), 3.87 - 3.92 (m, IH), 3.95 - 4.01 (m, 3H), 4.24 (t, 2H), 4.71 (t, 2H), 4.97 (t, IH), 6.73 (t, IH), 6.81 (s, IH), 7.11 (s, IH), 7.24 (s, IH), 7.27 - 7.30 (m, IH), 7.36 - 7.39 (m, IH), 8.12 (d, IH), 8.31 (d, IH), 8.72 (s, IH); m/z 464 (M+H)+.
The preparation of 2-(azetidin-l-ylcarbonyi)-5-bromopyridine was described earlier. The preparation of 3-hydroxy-N-(l-methyl-lH-pyrazol-3-yl)-5-[(3i?)-tetrahydrofuran-3- yloxy]benzamide is described below:
3 -hydroxy-N-( 1 -methyl- lH-pyrazol-3 -yl)-5 -Ff 3i?)-tetrahydrofuran-3 -yloxyibenzamide
Figure imgf000204_0002
A suspension of 3 -hydroxy-N-(l -methyl- lH-pyrazol-3-yl)-5- [(phenylmethyl)oxy]benzamide (450 mg, 1.39 mmol), (35)-tetrahydrofuran-3-yl 4- methylbenzenesulfonate (507 mg, 2.09 mmol) and potassium carbonate (481 mg, 3.48 mmol) in acetonitrile (5 mL) was stirred in a microwave reactor at 160°C for 3 hours. The solvent was removed in vacuo and ethyl acetate added. The organics were washed with water (40 mL), brine (40 mL), dried (MgSO4), filtered and the solvent removed in vacuo. The residue was chromatographed on silica, eluting with a gradient of 0-100% ethyl acetate in isohexane, to give N-(l-methyl-lH-pyrazol-3-yl)-3-[(phenylmethyl)oxy]-5- [(3i?)-tetrahydrofuran-3-yloxy]benzamide as a white foam. This was dissolved in ethanol (5 mL) and ammonium formate (182 mg, 2.88 mmol) added in one portion. The reaction was blanketed with argon and 10% palladium on activated carbon (30 mg) was added. This mixture was heated in a microwave reactor at 14O0C for 10 minutes, the catalyst filtered off and the volatiles removed in vacuo to give the title product as a white solid (305 mg). 1R NMR δ (de-DMSO): 1.96 - 2.00 (m, 1Η), 2.20 - 2.25 (m, 1Η), 3.74 - 3.85 (m, 6Η), 3.87 - 3.91 (m, IH), 5.06 - 5.08 (m, IH), 6.46 (t, IH), 6.57 (d, IH), 6.97 (s, IH), 7.02 (s, IH), 7.60 (d, IH), 9.74 (s, IH), 10.70 (s, IH); m/z 304 (M+H)+.
The preparation of 3-hydroxy-iV-(l -methyl- lH-pyrazol-3-yl)-5-[(phenylmethyl)oxy] benzamide was described earlier. (35)-Tetrahydrofuran-3-yl 4-methylbenzenesulfonate was prepared in an analogous fashion to (3i?)-tetrahydrofuran-3-yl 4- methylbenzenesulfonate, as described in Example 25.
Example 50: 3-{f5-(Azetidin-l-ylcarbonvnpyrazin-2-vIloxy>-5-{rflS)-l-methvI-2- (methyloxy)ethylloxyl-iV-pyridin-2-ylbenzamide
Figure imgf000205_0001
l-Chloro-N,N,2-trimethyl-l-propenylamine (0.080 mL, 0.6 mmol) was added to a solution of 3 - { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl] oxy } -5- { [( 1 S)- 1 -methyl-2- (methyloxy)ethyl]oxy}benzoic acid (193 mg, 0.50 mmol) in DCM (10 mL) and stirred at RT for 30 minutes. 2-Aminopyridine (57 mg, 0.6 mmol) and pyridine (0.082 mL, 1 mmol) were added and the reaction stirred for a further 16 hours. The solvent was removed in vacu and the residue was taken up in ethyl acetate (50 mL). The organic phase was washed with water (2 x 20 mL),lN citric acid (10 mL), water (10 mL), a saturated aqueous solution of sodium bicarbonate (10 mL) and brine (100 mL), then dried (MgSO4), filtered, and evaporated in vacuo. The residue was chromatographed on silica, eluting with a gradient of 50-80% ethyl acetate in isohexane, to give the desired compound as a white foam (42 mg). 1H NMR δ (CDCl3): 1.28 (d, 3H), 2.31 (quin, 2H), 3.34 (s, 3H), 3.43 - 3.56 (m, 2H), 4.19 (t, 2H), 4.57 (q, IH), 4.62 (t, 2H), 6.90 (s, IH), 7.03 (t, IH), 7.20 (s, IH), 7.36 (s, IH), 7.71 (t, IH), 8.23 (d, IH), 8.28 (s, IH)5 8.30 (d, IH), 8.64 (s, IH), 8.79 (s, lH); m/z 464 (M+H)+
The following compounds were prepared in an analogous fashion from the appropriate benzoic acid and the appropriate aminoheterocycle.
2.38 4.19 7.12 IH),
3H), 3.57 4.62 IH),
2.36 4.19 IH), (s,
(t,
(s,
Figure imgf000206_0001
5Oe 435 1H NMR δ (CDCl3): 1.31 (d, 6H), 2.32 (quin, (M+H)+ 2H), 4.19 (t, 2H), 4.56 (quin, IH), 4.62 (t, 2H), 6.85 (s, IH), 7.20 (s, IH), 7.30 (s, IH), 8.21 (s, IH), 8.29 (s, IH), 8.33 (s, IH), 8.40 (s, IH),
Figure imgf000207_0001
8.80 (s, IH), 9.61 (s, IH)
5Of 464 1HNMR δ (CDCl3): 1.27 (d, 3H), 2.29 (quin, (M+H)+ 2H), 3.34 (s, 3H), 3.43 - 3.55 (m, 2H), 4.18 (t, 2H), 4.56 (q, IH), 4.64 (t, 2H), 6.78 (s, IH), 7.09 (s, IH), 7.26 (s, IH), 7.32 (d, IH), 8.06 (d, IH), 8.22 (s, IH), 8.27 (s, IH), 8.34 (s, IH), 8.36 (s, IH), 9.60 (s, IH)
5Og* 468 1H NMR δ (CDCl3): 1.31 (d, 6H), 2.32 (quin, (M+H)+ 2H), 4.17 (t, 2H), 4.29 (t, 2H), 4.57 (quin, IH), 6.87 (s, IH), 7.20 (s, IH), 7.29 (s, IH), 8.10 (s, IH), 8.19 (s, IH), 8.21 (s, IH), 8.32 (s, IH),
Figure imgf000207_0002
8.42 (s, IH), 9.62 (s, IH)
5Oh 498 1H NMR δ (CDCl3): 1.29 (d, 3H), 2.32 (quin, (M+H)+ 2H), 3.35 (s, 3H), 3.43 - 3.57 (m, 2H), 4.17 (t, 2H), 4.29 (t, 2H), 4.57 (q, IH), 6.93 (s, IH), 7.24 (s, IH), 7.35 (s, IH), 8.10 (s, IH), 8.19 (s, IH), 8.21 (s, IH), 8.32 (s, IH), 8.42 (s, IH),
Figure imgf000207_0003
9.62 (s, IH)
* The isolated sample was contaminated with approximately 15% of an unknown impurity following initial chromatography.
The preparations of 3 - { [5 -(azetidin- 1 -ylcarbonyl)pyrazin-2-yl] oxy } -5 - { [( 1 S)- 1 -methyl-2- (methyloxy)ethyl]oxy}benzoic acid, 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-[(l- methylethyl)oxy]benzoic acid, 3-{ [6-(azetidin- 1 -ylcarbonyl)pyridin-3-yl]oxy}-5-{ [(1S)- 1 - methyl-2-(methyloxy)ethyl]oxy}benzoic acid, 3-{[5-(azetidin-l-ylcarbonyl)-3- chloropyridin-2-yl]oxy}-5-[(l-methylethyl)oxy]benzoic acid, and 3-{[5-(azetidin-l- ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(lS)-l-methyl-2-(methyloxy)ethyl]oxy}benzoic acid were described earlier. Example 51: S-lfS-Chloro-S-fmorphoIin^-vIcarbonvDpyridin^-vnoxyl-iV-fl-methyl- IH-pyr azoI-3-yl)-5- r(3S)-tetr ahydrof ur an-3-yIoxyl benzamide
Figure imgf000208_0001
Cesium carbonate (489 mg, 1.5 mmol) was added to a solution of 3-hydroxy-N-(l-methyl- lH-pyrazol-3-yl)-5-[(3>S)-tetrahydrofuran-3-yloxy]benzamide (152 mg, 0.5 mmol) and 4- [(5,6-dichloropyridin-3-yl)carbonyl]morpholine (143 mg, 0.55 mmol) in acetonitrile (5 mL) and the mixture heated at 12O0C in a microwave reactor for 2 hours. The acetonitrile was evaporated in vacuo and the residue partitioned between water (25 mL) and ethyl acetate (50 mL). The organic layer was washed with brine, dried (MgSO4) and evaporated to a residue which was chromatographed on silica, eluting with ethyl acetate, to give the desired compound (203 mg). 1R ΝMR δ (CDCl3): 2.05 - 2.2 (m, 2Η), 3.4 - 3.7 (m, 8H), 3.7 (s, 3H), 3.85 (m, 2H), 3.95 (d, 2H), 4.9 (m, IH), 6.7 (d, IH), 6.8 (d, IH), 7.15 (d, IH), 7.25 (d, 2H), 7.85 (d, IH), 8.0 (d, IH), 8.95 (s, IH); m/z 512 (M+H)+.
The following compound was prepared in an analogous fashion from 3-hydroxy-N-(l- methyl- lH-pyrazol-3-yl)-5-[(35)-tetrahydrofuran-3-yloxy]benzamide and 7-[(5,6- dichloropyridin-3 -yl)carbonyl] -7-azabicyclo [2.2.1 ]heptane
Figure imgf000208_0002
The preparation of 3-hydroxy-N-(l-methyl-lH-pyrazol-3-yl)-5-[(35)-tetrahydrofuran-3- yloxy]benzamide was described earlier. The preparation of 4-[(5,6-dichloropyridin-3-yl)carbonyl]moφholine is described below:
4-|T5.6-Dichloropyridm-3-yl)carbonyl]morpholine
Figure imgf000209_0001
Oxalyl chloride (3.2 mL; 36.0 mmol) was added to a solution of 5,6 dichloronicotinic acid (5.76 g; 30.0 mmol) and 4M hydrogen chloride in dioxane (7.5 mL; 30 mmol) in DCM (50 mL). The mixture was stirred at RT for 18 hours, the DCM evaporated in vacuo, the residue azeotroped with toluene (2 x 15 mL) and added to a solution of morpholine (3.1 mL; 36.0 mmol) and triethylamine (10.0 mL; 72 mmol) in DCM (150 mL). The mixture was stirred at RT for 18 hours, the DCM evaporated in vacuo, and the residue partitioned between water (75 mL) and ethyl acetate (100 mL). The organic layer was washed with brine, dried (MgSO4) and evaporated to a residue which was chromatographed on silica, eluting with 50% ethyl acetate in isohexane, to give the desired compound (7.1 g). 1H NMR δ (CDCl3): 3.3 - 3.8 (br, 8H), 7.8 (s, IH), 8.25 (s, IH); m/z 261 (M+H)+.
The preparation of 7-[(5,6-dichloropyridin-3-yl)carbonyl]-7-azabicyclo[2.2.1]heptane is described below:
7- [(5 ,6-Dichloropyridin-3-yl)carbonyl1 -7-azabicyclo [2.2.1 "|heptane
Figure imgf000209_0002
Oxalyl chloride (3.2 mL; 36.0 mmol) was added to a solution of 5,6 dichloronicotinic acid
(5.76 g, 30.0 mmol) and 4M hydrogen chloride in dioxane (7.5 mL; 30.0 mmol) in DCM (50 mL). The mixture was stirred at RT for 18 hours, the DCM evaporated in vacuo, the residue azeotroped with toluene (2 x 15 mL) and added to a solution of 7- azabicyclo[2.2. l]heptane hydrochloride (4.75 g; 36.0 mmol) and triethylamine (15.1 mL; 108 mmol) in DCM (150 mL). The mixture was stirred at RT for 18 hours, the DCM evaporated in vacuo and the residue partitioned between water (75 mL) and ethyl acetate (150 mL). The organic layer was washed with brine, dried (MgSO4) and evaporated to give a solid which was crystallised from ethyl acetate to give the desired compound (5.45 g). 1H NMR δ (CDCl3): 1.5 (m, 4H), 1.85 (m, 4H), 4.0 (br, IH), 4.65 (br, IH), 7.9 (s, IH), 8.4 (s, lH); m/z 271 (M+H)+.
Example 52: 3-{f5-(Azetidin-l-ylcarbonyl)-4-methyI-l.,3-thiazol-2-vnoxy}-N-(l- methyl-lH-pyrazoI-3-v0-5-r(3iSVtetrahydrofuran-3-yloxylbenzamide
Figure imgf000210_0001
Cesium carbonate (489 mg, 1.5 mmol) was added to a solution of 3-hydroxy-N-(l-methyl- lH-pyrazol-3-yl)-5-[(35)-tetrahydrofuran-3-yloxy]benzamide (152 mg, 0.5 mmol) and 5- (azetidin-l-ylcarbonyl)-2-bromo-4-methyl-l,3-thiazole (144 mg, 0.55 mmol) in acetonitrile (5 mL) and the stirred mixture heated at 12O0C in a microwave reactor for 2 hours. The mixture was allowed to cool, the acetonitrile evaporated in vacuo, and the residue partitioned between water (25 mL) and ethyl acetate (50 mL). The organic layer was washed with brine, dried (MgSO4) and evaporated to a residue which was chromatographed on silica, eluting with ethyl acetate, to give the desired compound as a solid which was further crystallised from an ethyl acetate / isohexane mixture (122 mg). 1H ΝMR δ (CDCl3): 2.0 - 2.15 (m, 2H), 2.2 - 2.3 (m, 2H), 2.5 (s, 3H), 3.75 (s, 3H), 3.85 (m, 2H), 3.95 (d, 2H), 4.1 -4.3 (m, 4H), 4.9 (m, IH), 6.75 (d, IH), 6.95 (d, IH), 7.25 (d, 2H), 7.3 (d, IH) and 8.8 (s, IH); m/z 484 (M+H)+.
The preparations of 3-hydroxy-N-(l-methyl-lH-pyrazol-3-yl)-5-[(35)-tetrahydrofuran-3- yloxy]benzamide and 5-(azetidin-l-ylcarbonyl)-2-bromo-4-methyl-l,3-thiazole were described earlier. Example 53 : 3-{ f6-f Azetidin-l-vIcarbonvnpyridin-3-yll oxyl-54 \( lS)-2-hvdroxy-l- methvIethyIloxy}-iV-(5-methylpyrazin-2-yl)benzamide
Figure imgf000211_0001
IM Hydrochloric acid (5 mL) was added to a solution of 3-{[6-(azetidin-l- ylcarbonyl)pyridin-3-yl]oxy}-5-[((15)-2-{[(l,l-dimethylethyl)(dimethyl)silyl]oxy}-l- methylethyl)oxy]-N-(5-methylpyrazin-2-yl)benzamide (82 mg, 0.14 mmol) in methanol (5 mL) and stirred at RT for 1 hour. The methanol was removed in vacuo and the residue taken to pH6, extracted into ethyl acetate (3 x 50 mL) and the combined organics washed with brine (50 mL), dried (MgSO4), filtered and reduced in vacuo. The crude yellow oil was chromatographed on silica, eluting with 0-5% methanol in ethyl acetate, to give the desired compound as a white foam (36 mg). The compound could be crystallised from a mixture of tert-butylmethyl ether and methanol. 1H ΝMR δ (CDCl3): 1.24 (d, 3H), 1.81 (s, IH), 2.28 (quin, 2H), 2.48 (s, 3H), 3.67 - 3.72 (m, 2H), 4.17 (t, 2H), 4.47 - 4.54 (m, IH), 4.63 (t, 2H), 6.73 (t, IH), 7.09 (t, IH), 7.25 (t, IH), 7.27 - 7.30 (m, IH), 8.01 - 8.06 (m, 2H), 8.24 (d, IH), 8.58 (s, IH), 9.44 (d, IH); m/z 464 (M+H)+
The following compound was synthesised in an analogous fashion from 3-{[6-(azetidin-l- ylcarbonyl)pyridin-3-yl]oxy}-5-[((lS)-2-{[(l,l-dimethylethyl)(dimethyl)silyl]oxy}-l- methylethyl)oxy]-N-(3 -methyl- 1 ,2,4-thiadiazol-5-yl)benzamide.
Figure imgf000211_0002
The preparation of 3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-[((15)-2-{[(l,l- dimethylethyl)(dimethyl)silyl]oxy}-l-meihyletliyl)oxy]-N-(5-methylpyrazin-2- yl)benzamide is described below.
3-1 [6-f Azetidin- 1 -ylcarbαnyltøyridin-3-yl1oxy}-5-IT( lS)-2-i [ClJ- dimethylethvDCdiniethvDsilv^oxyl-l-methylethvDoxyl-N-rS-methylpyrazin-Σ- vDbenzamide
Figure imgf000212_0001
l-Chloro-N,N,2-trimethyl-l-propenylamine (0.11 mL, 0.80 mmol) was added to a solution of 3-{ [6-(azetidin- 1 -ylcarbonyl)pyridin-3-yl]oxy }-5-[((15)-2-{ [(1 , 1- dimethylethyl)(dimethyl)silyl]oxy}-l-methylethyl)oxy]benzoic acid (0.3 g, 0.62 mmol) in DCM (10 mL) and stirred for 1 hour. 2-Amino-5-methylpyrazine (135 mg, 1.23 mmol), then pyridine (0.1 mL, 1.23 mmol), were added and the mixture stirred for a further 30 mins before being reduced in vacuo and partitioned between ethyl acetate (50 mL) and water (50 mL). The aqueous layer was further extracted into ethyl acetate (50 mL) and the combined organics washed with water (50 mL), brine (50 mL), dried (MgSO4), filtered and reduced in vacuo. The crude oil was chromatographed on silica, eluting with 40-100% ethyl acetate in isohexane, to give the desired compound as a colourless oil (82 mg). 1H ΝMR δ (CDCl3): 0.00 (s, 3H), 0.03 (s, 3H), 0.83 (s, 9H), 1.28 (d, 3H), 2.32 (quin, 2H), 2.53 (s, 3H), 3.64 - 3.77 (m, 2H), 4.22 (t, 2H), 4.47 - 4.51 (m, IH), 4.68 (t, 2H), 6.81 (t, IH), 7.10 (t, IH), 7.27 (t, IH), 7.33 - 7.35 (m, IH), 8.08 - 8.11 (m, 2H), 8.30 (d, IH), 8.37 (s, IH), 9.50 (d, IH); m/z 578 (M+H)+
3-{[6-(Azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-[((15)-2-{[(l,l- dimethylethyl)(dimethyl)silyl]oxy}-l-methylethyl)oxy]-N-(3-methyl-l,2,4-thiadiazol-5- yl)benzamide was prepared in an analogous fashion from 3-{[6-(azetidin-l- ylcarbonyl)pyridin-3-yl]oxy}-5-[((l«S)-2-{ [(1 , 1 -dimethylethyl)(dimethyl)silyl]oxy }- 1 - methylethyl)oxy]benzoic acid.
Figure imgf000213_0002
The preparation of 2-amino-5-methylpyrazine was described earlier.
The preparation of 3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3 -yljoxy } -5- [(( lS)-2- { [( 1 , 1 - dimethylethyl)(dimethyl)silyl]oxy}-l-methylethyl)oxy]benzoic acid is described below.
3-{r6-rAzetidin-l-ylcarbonvnpyridin-3-ylloxyl-5-r(a^-2-(rri.l- dimethylethyiydimethyDsilylloxyl-l-methylethvDoxylbenzoic acid
Figure imgf000213_0001
fert-Butyldimethylsilyl chloride (1.11 g, 7.36 mmol) was added to a mixture of 3-{[6- (azetidin- 1 -ylcarbonyl)pyridin-3 -yljoxy } -5- { [( 15)-2-hydroxy- 1 -methylethyl] oxy }benzoic acid (1.37g, 3.68 mmol) and l,8-diazabicyclo(5,4,0)undec-7-ene (1.11 mL, 7.36 mmol) in acetonitrile (20 mL) and stirred at RT for 24 hours. The solvent was removed in vacuo and water (80 mL) added to the resultant oil. The mixture was adjusted to pH 10 with IM sodium bicarbonate solution then washed with ether (80 mL) to remove impurities. The aqueous layer was adjusted to pH 3 with IM citric acid and extracted into ethyl acetate (2 x 100 mL) and the combined organics washed with brine (50 mL), dried (MgSO4), filtered and reduced in vacuo. The crude material was chromatographed on an Isolute NH2 cartridge, eluting with methanol then ammonia in methanol, to give a beige foam. This material was subsequently triturated with ethyl acetate to give the desired compound as a beige foam (1.0 g). 1H NMR δ (CDCl3): 0.01 (s, 3H), 0.03 (s, 3H), 0.83 (s, 9H)5 1.27 (d, 3H), 2.26 - 2.35 (m, 2H), 3.58 - 3.79 (m, 2H), 4.15 - 4.25 (m, 2H), 4.43 - 4.49 (m, IH), 4.60 - 4.69 (m, 2H), 6.77 - 6.82 (m, IH), 7.28 (m, 2H), 7.42 - 7.44 (m, IH), 8.02 - 8.07 (m, IH), 8.27 - 8.29 (m, IH); m/z 487 (M+H)+ B-lfό-CAzetidin-l-ylcarbonylipyridin-S-ylloxyl-S-lfriiS^-l-hydroxy-l- methylethylloxylbenzoic acid
Figure imgf000214_0001
A mixture of methyl 3-hydroxy-5-[(lS)-2-hydroxy-l-methylethoxy]benzoate (2.35 g, 10.39 mmol), 2-(azetidin-l-ylcarbonyl)-5-bromopyridine (2.51 g, 10.39 mmol), cesium carbonate (10.16 g, 31.16 mmol) and bromotris(triphenylphosphine)copper (1.93 g, 2.08 mmol) in DMA (20 mL) was stirred in a microwave reactor at 160°C for 8 hours. The mixture was partitioned between ethyl acetate (50 mL) and water (50 mL). The aqueous layer was acidified with IM citric acid and extracted into ethyl acetate (2 x 100 mL), washed with brine, dried (MgSO4) and the solvent removed in vacuo. The crude oil was chromatographed on an Isolute NH2 cartridge, eluting with methanol then ammonia in methanol, to give the desired material as a beige foam (1.37 g). m/z 373 (M+H)
The preparations of methyl 3-hydroxy-5-[(liS)-2-hydroxy-l-methylethoxy]benzoate and 2- (azetidin- 1 -ylcarbonyi)-5-bromopyridine were described earlier.
Example 54; S-irS-fAzetidin-l-vIcarbonvIVa-chloropyridin^-vIloxyl-S-ird.y)^- hvdroxy-l-methvIethylloxy|-N-(5-methylpyrazin-2-yl)benzamide
Figure imgf000214_0002
IM Hydrochloric acid (10 mL) was added to a solution of 3-{[5-(azetidin-l-ylcarbonyl)-3- chloropyridin-2-yl]oxy}-5-[((llS)-2-{[(l,l-dimethylethyl)(dimethyl)silyl]oxy}-l- methylethyl)oxy]-N-(5-methylpyrazin-2-yl)benzamide (180 mg, 0.29 mmol) in methanol (10 mL) and stirred at RT for 1 hour. The methanol was removed in vacuo and the residue adjusted to pH 6 then extracted into ethyl acetate (3 x 50 mL). The combined organics were washed with brine (50 mL), dried (MgSO4), and reduced in vacuo to give a yellow oil which was chromatographed on silica, eluting with 0-5% methanol in ethyl acetate, to give the desired compound as a white foam (134 mg). The compound was crystallised from ethyl acetate and methanol.
1H NMR δ (CDCl3): 1.33 (d, 3H), 2.40 (quin, 2H), 2.56 (s, 3H), 3.76 - 3.81 (m, 2H), 4.25 (t, 2H), 4.38 (t, 2H), 4.57 - 4.64 (m, IH), 6.98 (t, IH), 7.31 (t, IH), 7.42 (t, IH), 8.14 (s, IH), 8.17 (d, IH), 8.26 (d, IH), 8.64 (s, IH), 9.55 (s, IH); w/z 498 (M+H)+
The following compounds were prepared in an analogous fashion:
Figure imgf000215_0001
The preparation of 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-[((15)-2- {[(1,1 -dimethylethyl)(dimethyl)silyl]oxy } - 1 -methylethyl)oxy]-N-(5-methylpyrazin-2- yl)benzamide is described below. S-irS-rAzetidin-l-ylcarbonylVS-chloropyridin-Σ-ylloxyl-S-rrri^^-irri.l- dimethylethviydimethvDsilylloxyl-l-niethylethvDoxyl-N-fS-methylpyrazin^- vDbenzamide
Figure imgf000216_0001
l-Chloro-N,N,2-trimethyl-l-propenylamine (0.1 mL, 0.75 mmol) was added to a solution of3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-[((15)-2-{[(l,l- dimethylethyl)(dimethyl)silyl]oxy}-l-meth.ylethyl)oxy]benzoic acid (0.3 g, 0.58 mmol) in DCM (10 mL) and stirred for 1 hour. 2-Amino-5-methylpyrazine (126 mg, 1.15 mmol) then pyridine (0.094 mL, 1.15 mmol) were added and the mixture stirred for a further 30 mins before being reduced in vacuo and partitioned between ethyl acetate (50 mL) and water (50 mL). The aqueous layer was further extracted into ethyl acetate (50 mL) and the combined organics washed with water (50 mL), brine (50 mL), dried (MgSO4), and reduced in vacuo. The crude oil was chromatographed on silica, eluting with 40-100% ethyl acetate in isohexane, to give the title compound as an oil (180 mg). 1H ΝMR δ (CDCl3): 0.00 (s, 3H), 0.03 (s, 3H), 0.82 (s, 9H), 1.28 (d, 3H), 2.35 (quin, 2H), 2.51 (s, 3H), 3.63 - 3.78 (m, 2H), 4.19 (t, 2H), 4.32 (t, 2H), 4.47 - 4.51 (m, IH), 6.95 (t, IH), 7.23 - 7.24 (m, IH), 7.35 (t, IH), 8.08 (s, IH), 8.12 (d, IH), 8.21 (d, IH), 8.45 (s, IH), 9.51 (d, IH); m/z 612 (M+H)+
The following intermediates used in the preparation of Examples 54a-c were prepared in an analogous fashion. Structure m/z NMR
618 1H NMR δ (CDCl3): 0.00 (s, 3H), 0.03 (s, 3H), 0.84 (s, (M+H)+ 9H), 1.37 (d, 3H)3 2.30 - 2.38 (m, 5H), 3.61 - 3.79 (m, 2H), 4.19 (t, 2H), 4.30 (t, 2H), 4.41 - 4.47 (m, IH), 6.84 -
Figure imgf000217_0001
6.86 (m, IH), 7.62 - 7.62 (m, IH), 7.72 - 7.73 (m, IH), 8.10 - 8.11 (m, IH), 8.19 - 8.20 (m, IH), 9.89 (s, IH)
598 1HNMR δ (CDCl3): 0.00 (s, 3H), 0.03 (s, 3H), 0.83 (s, (M+H)+ 9H), 1.29 (d, 3H), 2.35 (quin, 2H), 3.64 - 3.79 (m, 2H), 4.19 (t, 2H), 4.32 (t, 2H), 4.47 - 4.51 (m, IH), 6.96 (s,
Figure imgf000217_0002
IH), 7.25 (s, IH), 7.36 (s, IH), 8.12 (d, IH), 8.21 (d, IH), 8.23 (s, IH), 8.34 (d, IH), 8.52 (s, IH), 9.64 (s, IH)
597 1HNMR δ (CDCl3): 0.00 (s, 3H), 0.03 (s, 3H), 0.84 (s, (M+H)+ 9H), 1.28 (d, 3H), 2.35 (quin, 2H), 3.63 - 3.78 (m, 2H), 4.19 (t, 2H), 4.32 (t, 2H), 4.46 - 4.50 (m, IH), 6.93 (t,
Figure imgf000217_0003
IH), 7.01 - 7.05 (m, IH), 7.23 - 7.23 (m, IH), 7.34 (t, IH), 7.69 - 7.73 (m, IH), 8.12 (d, IH), 8.21 (d, IH), 8.24 (d, IH), 8.31 (d, IH), 8.56 (s, IH)
The preparation of 3-{ [5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-[((l)S)-2- {[(l,l-dimethylethyl)(dimethyl)silyl]oxy}-l-methyleihyl)oxy]benzoic acid is described below.
3- { [5-(Azetidin- 1 -ylcarbonyr)-3-chloropyridin-2-yl1oxy| -5-\(( 1 S)-2- ( U 1.1 - dimethylethyiydimethypsilylioxyl - 1 -methylethvDoxy~|benzoic acid
Figure imgf000217_0004
tert-Butyldimethylsilyl chloride (2.78 g, 18.4 mmol) was added to a mixture of 3-{ [5- (azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl] oxy } -5- { [( 15)-2-hydroxy- 1 - methylethyl]oxy}benzoic acid (3.74 g, 9.19 mmol) and l,8-diazabicyclo(5,4,0)undec-7-ene (2.75 mL, 7.36 mmol) in acetonitrile (40 mL) and stirred at RT for 24 hours. The solvent was removed in vacuo and water (80 mL) added to the resultant oil. The mixture was adjusted to pH 10 with IM sodium bicarbonate solution and washed with ether to remove any impurities. The aqueous layer was adjusted to pH 3 with IM citric acid and extracted into ethyl acetate (2 x 100 mL). The combined organics were washed with brine (50 mL), dried (MgSO4), filtered and reduced in vacuo to give the desired compound as a white foam (2.9 g).
1R NMR δ (CDCl3): 0.00 (s, 3H), 0.03 (s, 3H), 0.83 (s, 9H), 1.28 (d, 3H), 2.35 (quin, 2H), 3.62 - 3.78 (m, 2H), 4.21 (t, IH), 4.32 (t, IH), 4.43 - 4.50 (m, IH), 6.95 - 6.98 (m, IH), 7.42 - 7.45 (m, IH), 7.50 - 7.53 (m, IH), 8.12 (d, IH), 8.22 (d, IH); m/z 521 (M+H)+
3 - I F5-f Azetidin- 1 - ylcarbonylV3 -chloropyridin-2-ylloxy) -5 - I \(l ^-2-hvdroxy- 1 - methylethylioxylbenzoic acid
Figure imgf000218_0001
Lithium hydroxide monohydrate (529 mg, 12.59 mmol) in water (25 mL) was added to a solution of methyl 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(liS)-2- hydroxy-l-methylethyl]oxy}benzoate (5.3 g, 12.6 mmol) in THF (50 mL) and stirred at RT for 3 hours. The THF was removed in vacuo and the aqueous layer was washed with ethyl acetate (50 mL) to remove any impurities. The aqueous layer was acidified and extracted into ethyl acetate (2 x 50 mL), and the organics washed with brine, dried (MgSO4), and concentrated in vacuo to give the desired compound as a white foam (3.74 g).
1U NMR δ (CDCl3): 1.24 (d, 3H), 2.31 (quin, 2H), 3.65 - 3.72 (m, 2H), 4.17 (t, 2H), 4.29 (t, 2H), 4.47 - 4.54 (m, IH), 6.93 (t, IH), 7.40 (s, IH), 7.46 (s, IH), 8.08 (d, IH), 8.19 (d, lH); m/z407 (M+H)+ Methvπ-irS-razetidin-l-ylcarbonylVS-chloropyridin-Σ-ylioxyl-S-irri^-l-hvdroxy-l- methylethylloxylbenzoate
Figure imgf000219_0001
A mixture of methyl 3-hydroxy-5-[(lS)-2-hydroxy-l-methylethoxy]benzoate (2.35 g, 10.39 mmol), 5-(azetidin-l-ylcarbonyl)-2,3-dichloropyridine (2.41 g, 10.39 mmol) and potassium carbonate (2.87 g, 20.8 mmol) in acetonitrile (20 mL) was stirred in a microwave reactor at 16O0C for 6 hours. The solvent was removed in vacuo and the residue partitioned between ethyl acetate (50 mL) and water (50 mL). The organic layer was washed with brine (50 mL), dried (MgSO4), and the solvent removed in vacuo. The crude oil was chromatographed on silica, eluting with 30-100% ethyl acetate in isohexane, to give the desired compound as a yellow oil (5.3 g).
1H NMR δ (CDCl3): 1.24 (d, 3H), 1.94 (t, IH), 2.32 (quin, 2H), 3.65 - 3.72 (m, 2H), 3.83 (s, 3H), 4.16 (t, 2H), 4.28 (t, 2H), 4.47 - 4.54 (m, IH), 6.90 (t, IH), 7.37 - 7.38 (m, IH), 7.43 - 7.45 (m, IH), 8.08 (d, IH), 8.18 (d, IH); m/z All (M+H)+
The preparations of methyl 3-hydroxy-5-[(15)-2-hydroxy-l-methylethoxy]benzoate and 5- (azetidin-l-ylcarbonyl)-2,3-dichloropyridine were described earlier.
Example 55: 3-{f5-(Azetidin-l-vIcarbonyl)pyridin-2-ylloxyl-5-{f(15)-2-hvdroxy-l- methvIethylloxy>-iV-(3-methyl-l,2,4-thiadiazol-5-vI)benzamide
Figure imgf000219_0002
A mixture of 3-hydroxy-5-{[(l,S)-2-hydroxy-l-methylethyl]oxy}-N-(3-methyl-l,2,4- thiadiazol-5-yl)benzamide (237 mg, 0.77 mmol), 5-(azetidin-l-ylcarbonyl)-2- chloropyridine (151 mg, 0.77 mmol) and potassium carbonate (213 mg, 1.54 mmol) in acetonitrile (5 mL) was stirred in a microwave reactor at 14O0C for 5 hours and at 1600C for 10 hours. The mixture was reduced in vacuo and ethyl acetate (50 mL) added. The mixture was washed with water (50 mL), brine (50 mL), dried (MgSO4), and reduced in vacuo to give a brown oil which was chromatographed on silica, eluting with 0-10% methanol in ethyl acetate, to give the desired compound as a white foam (64 mg).
1H NMR δ (CDCl3): 1.29 (d, 3H), 2.38 (quin, 2H), 2.49 (s, 3H), 2.72 (s, IH), 3.75 - 3.77 (m, 2H), 4.20 - 4.41 (m, 4H), 4.51 - 4.59 (m, IH), 6.95 (t, IH), 6.99 (d, IH), 7.35 (t, IH), 7.37 - 7.38 (m, IH), 8.05 - 8.08 (m, IH), 8.40 (d, IH)3 11.08 (s, IH); m/z 470 (M+H)+
The preparation of 5-(azetidin- 1 -ylcarbonyl)-2-chloropyridine was described earlier.
The preparation of 3-hydroxy-5-{[(15)-2-hydroxy-l-methylethyl]oxy}-N-(3-methyl-l,2,4- thiadiazol-5-yl)benzamide is described below.
3-Hvdroxy-5-(rCl^-2-hvdroxy-l-methylethylloxyl-N-('3-methyl-1.2.4-thiadiazol-5- ypbenzamide
Figure imgf000220_0001
Iodotrimethylsilane (5.51 mL, 38.7 mmol) was added to 3-hydroxy-5-{[(15)-2-methoxy- (l-methylethyl)oxy}-N-(3-methyl-l,2,4-thiadiazol-2-yl)benzamide (2.5 g, 7.73 mmol) in acetonitrile (25 mL) and the reaction stirred at RT for 48 hours. Methanol (15 mL) was added and the reaction stirred for 1 hour then a saturated solution of sodium thiosulphate (10 mL) was added and stirred for 20 mins. The volatiles were removed in vacuo and the aqueous residue extracted into ethyl acetate (2 x 150 mL). The organics were washed with water, brine, dried (MgSO4), and reduced in vacuo to give a yellow solid. The solid was triturated with DCM and then with ethyl acetate to give the desired compound as a white solid (1.44 g). 1H ΝMR δ (d6-DMSO): 1.23 (d, 3H), 2.49 (s, 3H), 3.46 - 3.59 (m, 2H), 4.48 - 4.52 (m, IH), 4.89 (t, IH), 6.60 (s, IH), 7.08 (s, IH), 7.24 (s, IH), 9.91 (s, IH), 13.28 (s,
Figure imgf000220_0002
The preparation of 3-hydroxy-5-{[(15)-2-methoxy-(l-methylethyl)oxy}-N-(3-methyl- 1 ,2,4-thiadiazol-2-yl)benzamide was described earlier. Example 56 : N-(l-methyl-lfr-pyrazol-3-yl)-3-{ [6-(pyrrolidin-l-vIcarbonyl)pyridin-3- ylloxy>-5-r(3S)-tetrahvdrofuran-3-yloxylbenzamide
Figure imgf000221_0001
Copper (I) iodide (95 mg, 0.5 mmol), 2,2,6,6-tetramethyl-3,5-heptanedione (0.42 mL, 2.0 mmol) and cesium carbonate (489 mg, 1.5 mmol) were added to a solution of 3-hydroxy- N-(l-methyl-lH-pyrazol-3-yl)-5-[(35)-tetrahydrofuran-3-yloxy]benzamide (152 mg, 0.5 mmol) and 5-bromo-2-(pyrrolidin-l-ylcarbonyl)pyridine (141 mg, 0.55 mmol) in ΝMP (5 mL) and the stirred mixture heated at 16O0C in a microwave reactor for 12 hours. The mixture was cooled to RT and pressure, the mixture partitioned between water (75 mL) and ethyl acetate (50 mL), the organic layer washed with brine, dried (MgSO4) and evaporated to a residue which was chromatographed on silica, eluting with ethyl acetate, to give the desired material (81 mg).
1H ΝMR δ (CDCl3): 1.8 - 1.95 (m, 4H), 2.0 - 2.2 (m, 2H), 3.6 (m, 2H), 3.75 (s, 3H), 3.85 (m, 2H), 3.95 (m, 2H), 4.9 (m, IH), 6.65 (m, IH), 6.75 (m, IH), 7.1 (m, IH), 7.2 (s, IH), 7.25 (m, IH), 7.3 (dd, IH), 7.8 (d, IH), 8.25 (d, IH), 8.8 (s, IH); m/z 478 (M+H)+.
The preparation of 3 -hydroxy- N-( 1 -methyl- lH-pyrazol-3 -yl)-5- [(35)-tetrahydrofuran-3 - yloxyjbenzamide was described earlier.
The preparation of 5-bromo-2-(pyrrolidin-l-ylcarbonyl)pyridine is described below.
5-Bromo-2-("r)yrrolidin- 1 -ylcarbonvDpvridine
Figure imgf000221_0002
Oxalyl chloride (0.83 mL; 9.3 mmol) and DMF (1 drop) were added to a solution of 5- bromoisonicotinic acid (1.57 g; 7.75 mmol) and 4M hydrogen chloride in dioxan (1.93 mL; 7.75 mmol) in DCM (50 mL). The mixture was stirred at RT for 18 hours, the DCM removed in vacuo and the residue azeotroped with toluene (2 x 15 mL). The residue was and added to a solution of pyrrolidine (0.78 mL; 9.3 mmol) and triethylamine (2.6 mL; 18.6 mmol) in DCM (40 mL) and the mixture stirred at RT for 6 hours. The DCM was removed in vacuo, the residue partitioned between water (75 mL) and ethyl acetate (100 mL), the organic layer washed with brine, dried (MgSO4) and evaporated to a residue which was chromatographed on silica, eluting with 50% ethyl acetate in iso hexane, to give the desired material (1.40 g). 1U NMR δ (CDCl3): 1.85 (m, 4H), 3.6 (m, 2H), 3.7 (s, 3H), 7.7 (d, IH)5 7.85 (s, IH), 8.55 (s, IH); m/z 257 (M+H)+.
Example 57 ; 3-{ [6-(Azetidin-l-ylcarbonyI)pyridin-3-yIl oxy }-iV-pyr azin-2-yl-5- [(3S)- tetrahvdrofuran-3-yloxylbenzamide
Figure imgf000222_0001
l-Chloro-N,N,2-trimethyl-l-propenylamine (0.095 mL, 0.75 mmol) was added to a solution of 3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-[(35)-tetrahydrofuran-3- yloxy]benzoic acid (250 mg, 0.65 mmol) in DCM (6 mL) and stirred at RT for 30 - 40 minutes. 2-Aminopyrazine (125 mg, 1.31 mmol) and pyridine (0.106 mL, 1.31 mmol) were added and the reaction stirred at RT for 2 hours. The solvent was removed in vacuo, water (20 mL) was added and the mixture was extracted with ethyl acetate (3 x 20 mL). The organic extracts were combined and washed with 2Ν hydrochloric acid (20 mL), a saturated solution of sodium bicarbonate (20 mL), water (20 mL), brine (20 mL), dried (MgSO4) and evaporated in vacuo. The crude product was chromatographed on silica, eluting with a gradient of 0-10% methanol in DCM, to give the desired compound (71 mg). 1H NMR δ (CDCl3): 2.13 - 2.30 (m, 2H), 2.32 - 2.39 (m, 2H), 3.89 - 4.04 (m, 4H), 4.25 (t, 2H), 4.71 (t, 2H), 4.97 - 5.01 (m, IH), 6.77 (t, IH), 7.16 (t, IH), 7.25 (s, IH), 7.40 (dd, IH), 8.14 (d, IH), 8.28 (dd, IH), 8.34 (d, IH), 8.38 (s, IH), 8.40 (d, IH), 9.66 (d, IH); m/z 462 (M+H)+, 460 (M-H)'
The following compounds were prepared in an analogous fashion from 3-{[6-(azetidin-l- ylcarbonyl)pyridin-3-yl]oxy}-5-[(35)-tetrahydrofuran-3-yloxy]benzoic acid and either 1,1- dimethylethyl S-amino-S-methyl-lH-pyrazole-l-carboxylate or 1,1-dimethylethyl 3- amino-lH-pyrazole-1-carboxylate. The material produced was dissolved in acetonitrile (3mL) and heated at 15O0C in a microwave reactor for 10 minutes to give the desired material after chromatography.
Figure imgf000223_0002
The preparations of 3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-[(35)- tetrahydrofuran-3-yloxy]benzoic acid and 1,1-dimethylethyl 3-amino-lH-pyrazole-l- carboxylate were described earlier.
The preparation of 1,1-dimethylethyl 3-amino-5-methyl-lH-pyrazole-l-carboxylate is described below.
1 , 1 -Dimethylethyl 3-ammo-5-methyl- lH-pyrazole- 1 -carboxylate
Figure imgf000223_0001
5-Methyl-lH-pyrazol-3-amine (800 mg, 8.25 mmol) was dissolved in DMF (10 mL) at 0 0C and treated with sodium hydride (336 mg, 8.25 mmol) followed by stirring for a further 30 minutes. Warmed di-fert-butyl dicarbonate (1.80 g, 8.25 mmol) was then slowly added via syringe over 5 min and the reaction was allowed to warm to RT and stirred for a further 1 hour. The reaction was taken up in saturated aqueous sodium hydrogencarbonate (50 mL) and ethyl acetate (100 mL). The organic layer was separated then dried (MgSO4), filtered and evaporated. Purification by column chromatography, eluting with 50-100% ethyl acetate in isohexane, afforded the title compound as a colourless oil (380 mg). 1E NMR δ (CDCl3): 1.62 (s, 9H), 2.43 (s, 3H), 3.87 (br. s, 2H), 5.60 (s, IH)
Example 58 : 3-{ f 5-(Azetidin-l-yIcarbonyl)-3-chlorop yridin-2-vn oxy)-5-{ [2-fluoro-l- (fluoromethyl)ethylloxyl-7V-(5-methvIpyrazin-2-yl)benzamide
Figure imgf000224_0001
l-Chloro-N,N-2-trimethylpropenylamine (0.08 mL, 0.60 mmol) was added to a solution of 3 - { [5-(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl] oxy } -5 - { [2-fluoro- 1 - (fluoromethyl)ethyl]oxy}benzoic acid (215 mg, 0.5 mmol) in DCM (5 mL) and the reaction stirred at RT for 30 - 40 minutes. Pyridine (0.08 mL, 1.0 mmol) and 2-amino-5- methylpyrazine (108 mg, 1.0 mmol) were added and the reaction stirred for 16 hours before being concentrated in vacuo and water (20 mL) added. The mixture was extracted with ethyl acetate (3 x 20 mL), washed with IN hydrochloric acid (20 mL), a saturated solution of sodium hydrogen carbonate (20 mL), brine (20 mL), dried (MgSO4) and concentrated in vacuo. The crude product was chromatographed on silica, eluting with a gradient of 0-10% methanol in DCM, to give the desired compound as a white solid (150 mg). 1H ΝMR δ (CDCl3): 2.40 (quintet, 2H), 2.56 (s, 3H), 4.22-4.31 (m, 2H), 4.32-4.40 (m, 2H), 4.62 - 4.82 (m, 5H), 7.06 (t, IH), 7.38 (t, IH), 7.47 (t, IH), 8.15 (s, IH), 8.17 (s, IH), 8.25 (d, IH), 8.46 (s, IH), 9.55 (s, IH); m/z 518 (M+H)+, 516 (M-H)"
The following compounds were prepared in an analogous fashion from the appropriate benzoic acid and either 2-amino-5-methylpyrazine, 1,1-dimethylethyl 3-amino-5-methyl- lH-pyrazole-1-carboxylate or 1,1-dimethylethyl 3-amino-lH-pyrazole-l-carboxylate. In the instances where either 1,1-dimethylethyl 3-amino-5-methyl-lH-pyrazole-l-carboxylate or 1,1-dimethylethyl 3-amino-lH-pyrazole-l-carboxylate were used, an additional step was required to obtain the desired material. This additional step required the material to be dissolved in acetonitrile (3mL) and heated at 15O0C in a microwave reactor for 10 minutes, the desired material was then isolated after chromatography.
(t,
IH) (quin, (m,
6.65
IH),
4.63 - - 8.37
Figure imgf000225_0001
The preparations of 1,1-dimethylethyl 3-amino-5-methyl-lH-pyrazole-l-carboxylate or 1,1-dimethylethyl 3-amino-lH-pyrazole-l-carboxylate were described earlier. The preparation of 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[2-fluoro- l-(fluoromethyl)ethyl]oxy}benzoic acid is described below. 3-([5-rAzetidin-l-ylcarbonylV3-chloropyridin-2-yl1oxyl-5-(r2-fluoro-l- (fluoromethyl)ethyl"loxy}benzoic acid
Figure imgf000226_0001
A mixture of methyl 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[2- fluoro-l-(fluoromethyl)ethyl]oxy}benzoate (3.6 g, 8.2 mmol) in THF (50 mL) and water (50 mL) was treated with IM sodium hydroxide solution (8.2 mL, 8.2 mmol) and allowed to stir at RT for 210 minutes. The THF was removed in vacuo, the residue filtered and extracted with ethyl acetate (30 mL). The aqueous layer was acidified with 2N hydrochloric acid, extracted with ethyl acetate (3 x 50 mL) and the combined organic extracts washed with water (10 mL), brine (10 mL) and concentrated in vacuo to give the desired material as a pale yellow foam (2.84 g). 1H NMR δ (CDCl3): 2.36 - 2.44 (m, 2H), 4.26 (t, 2H), 4.37 (t, 2H), 4.62 - 4.79 (m, 5H), 7.08 (t, IH), 7.56 - 7.57 (m, IH), 7.58 - 7.59 (m, IH)3 8.17 (d, IH), 8.27 (d, IH); m/z426 (M+H)+, 424 (M-H)"
3-{[5-(Azetidin-l-ylcarbohyl)pyrazin-2-yl]oxy}-5-{[2-fluoro-l-
(fluoromethyl)ethyl]oxy}benzoic acid was prepared in an analogous fashion from methyl 3- { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy } -5- { [2-fluoro- 1 - (fluoromethyl)ethyl] oxy } benzoate
Figure imgf000226_0002
The preparation of methyl 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[2- fluoro-l-(fluoromethyl)ethyl]oxy}benzoate is described below. Methyl 3-{r5-(azetidin-l-ylcarbonylV3-chloropyridin-2-ylloxy>-5-{r2-fluoro-l- ("fluoromethypethylloxylbenzoate
Figure imgf000227_0001
A solution of methyl 3-{[2-fluoro-l-(fluoromethyl)ethyl]oxy}-5-hydxoxybenzoate (1.78 g, 7.24 mmol), potassium carbonate (2.0 g, 14.5 mmol) and 5-(azetidin-l-ylcarbonyl)-2,3- dichloropyridine (2.08 g, 8.7 mmol) in acetonitrile (15mL) was heated in a microwave reactor at 130°C for 1 hour. The solution was diluted with ethyl acetate (150 mL), washed with water (3 x 50 mL), brine (20 mL), dried (MgSO4), and the solvent removed in vacuo to give the desired material as an orange oil (3.6 g). 1H NMR δ (CDCl3): 2.35 - 2.44 (m, 2H), 3.91 (s, 3H), 4.24 - 4.27 (m, 2H), 4.34 - 4.37 (m, 2H), 4.62 - 4.81 (m, 5H), 7.04 (t, IH), 7.51 (t, IH), 7.55 (m, IH), 8.15 (d, IH), 8.25 (d, IH); m/z 441 (M+H)+
Methyl 3- { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy }-5- { [2-fluoro- 1 - (fluoromethyl)ethyl]oxy}benzoate was prepared in an analogous fashion from methyl 3- { [2-fluoro- 1 -(fluoromethyl)ethyl]oxy } -5-hydroxybenzoate.
Figure imgf000227_0002
The preparation of methyl 3-{[2-fluoro-l-(fluoromethyl)ethyl]oxy}-5-hydroxybenzoate is described below. Methyl 3 - { ["2-fluoro- 1 -f fluoromethyltethyll oxy } -5-hydroxybenzoate
Figure imgf000228_0001
10% Palladium on charcoal (Pd/C; 50 mg) was added to a mixture of methyl 3-{[2-fluoro- l-(fluoromethyl)ethyl]oxy}-5-[(phenylmethyl)oxy]benzoate (1.2 g, 3.62 mmol) and ammonium formate (500 mg, 8.0 mmol) in ethanol (7 mL) and then heated at 14O0C for 20 minutes in a microwave reactor. The reaction was repeated on the same scale and the two reaction mixtures combined, filtered and filtrate concentrated in vacuo to give the desired material as a pale yellow oil (2.1 g). 1H NMR δ (CDCl3): 3.82 (s, 3H), 4.58 - 4.77 (m, 5H), 6.72 (t, IH), 7.17 - 7.22 (m, 2H); m/z 245 (M-H)"
The preparation of methyl 3-{[2-fluoro-l-(fluoromethyl)ethyl]oxy}-5- [(phenylmethyl)oxy]benzoate was described earlier.
Example 59: S-lfS-fAzetidin-l-ylcarbonvDpyrazin-Σ-vIloxyl-S-lfdiS^^-hvdroxy-l- methylethvIloxy}-iV-(5-methylpyrazin-2-yl)benzamide
Figure imgf000228_0002
IM Hydrochloric acid (5 mL) was added to a solution of 3-{[5-(azetidin-l- ylcarbonyl)pyrazin-2-yl] oxy } -5- [(( 1 S)-2- { [( 1 , 1 -dimethylethyl)(dimethyl)silyl] oxy} - 1 - methylethyl)oxy]-N-(5-methylpyrazin-2-yl)benzamide (90 mg, 0.16 mmol) in methanol (5 mL) and the mixture stirred at RT for 1 hour. The methanol was removed in vacuo, the residue adjusted to pH6 and then extracted into ethyl acetate (3 x 50 mL). The combined organics were washed with brine (50 mL), dried (MgSO4), and reduced in vacuo to give a yellow oil which was chromatographed on silica, eluting with 0-5% methanol in ethyl acetate, to give the desired compound as a white foam (28 mg). 1H NMR δ (CDCl3): 1.33 (d, 3H), 2.11 (s, IH), 2.38 (quin, 2H), 2.55 (s, 3H), 3.74 - 3.82 (m, 2H), 4.26 (t, 2H), 4.55 - 4.62 (m, IH), 4.69 (t, 2H), 6.97 (t, IH), 7.30 (t, IH), 7.40 (t, IH), 8.13 (s, IH), 8.35 (d, IH), 8.41 (s, IH), 8.85 (d, IH), 9.53 (d, IH); m/z465 (M+H)+
The preparation of 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-[((liS)-2-{[(l,l- dimethylethyl)(dimethyl)silyl]oxy}-l-methylethyl)oxy]-N-(5-methylpyrazin-2- yl)benzamide is described below.
3-1 r5-(Azetidin- 1 -ylcarbonvnpyrazin-2-yrioxy}-5-ITQ.SV2-{ Td .1 - dimethylethyiχdimethyl)silyl]oxy } - 1 -methylethyl)oxy]-N-f 5-methylpyrazin-2- ypbenzamide
Figure imgf000229_0001
A mixture of S-røl^^-ICCl.l-dimethylethyOCdimethy^silyyoxyJ-l-methylethy^oxyl-S- hydroxy-N-(5-methylpyrazin-2-yl)benzamide (0.12 g, 0.29 mmol), 2-(azetidin-l- ylcarbonyl)-5-chloropyrazine (57 mg, 0.29 mmol) and potassium carbonate (80 mg, 0.57 mmol) in acetonitrile (5 mL) was stirred in a microwave reactor at 140°C for 1 hour. The mixture was reduced in vacuo and ethyl acetate (50 mL) added. The mixture was washed with water (50 mL), brine (50 mL), dried (MgSO4), filtered and reduced in vacuo to give a brown oil which was chromatographed on silica, eluting with 50 to 100% ethyl acetate in isohexane, to give the desired compound as a colourless oil (90 mg). 1H ΝMR δ (CDCl3): - 0.03 (s, 3H), 0.00 (s, 3H), 0.81 (s, 9H), 1.26 (d, 3H), 2.32 (quin, 2H), 2.49 (s, 3H), 3.61 - 3.76 (m, 2H), 4.20 (t, 2H), 4.46 (sextet, IH), 4.62 (t, 2H), 6.91 (t, IH), 7.21 (t, IH), 7.32 (t, IH), 8.07 (s, IH), 8.28 (d, IH), 8.30 (s, IH), 8.79 (d, IH), 9.48 (d, IH). m/z 579 (M+H)+ 3-rrri^-2-(rri.l-Dimethylethviydimethvnsilvnoxy>-l-methylethvnoxyl-5-hvdroxy-N-(5- methylpyrazin-2-yPbenzamide
Figure imgf000230_0001
Lithium hydroxide monohydrate (0.38 g, 9.12 mol) in water (20 mL) was added to a solution of 3-{ [(I , l-dimethylethyl)(dimethyl)silyl]oxy }-5-[((15)-2-{ [(1 , 1 - dimethylethyl)(dimethyl)silyl]oxy } - 1 -methylethyl)oxy]-N-(5-methylpyrazin-2- yl)benzamide (1.94 g, 3.65 mol) in THF (40 mL) and stirred at RT for 20 hours. The THF was removed in vacuo and the aqueous layer was adjusted to pH7. Ethyl acetate (50 mL) and water (50 mL) were added then the aqueous layer re-extracted into ethyl acetate (50 mL) and the combined organics washed with water (50 mL), brine (50 mL), dried
(MgSO4), filtered and reduced in vacuo. The crude material was chromatographed on silica, eluting with 20-50% ethyl acetate in isohexane, to give the desired material as a white solid (0.8 g). 1R ΝMR δ (CDCl3): 0.04 (s, 3H), 0.08 (s, 3H), 0.87 (s, 9H), 1.30 (d, 3H), 2.56 (s, 3H), 3.65 - 3.81 (m, 2H), 4.46 - 4.51 (m, IH), 5.63 (s, IH), 6.63 (t, IH), 6.98 (t, IH), 7.04 (t, IH), 8.15 (s, IH), 8.41 (s, IH), 9.57 (s, IH); m/z 418 (M+H)+
3-(T(U -Dimethylethyl)rdimethvnsilylloxy}-5-rrri.y)-2-( ITl .1 - dimethylethyl)(dimethyl)silylloxy}-l-methylethyl)oxyl-N-(5-methylpyrazin-2- ypbenzamide
Figure imgf000230_0002
tert-Butyldimethylsilyl chloride (2.61 g, 17.31 mmol) was added to a solution of 3- hydroxy-5-{ [(l<S)-2-hydroxy-l-methylethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide (1.5 g, 4.95 mmol) and imidazole (2.36 g, 34.62 mmol) in DMF (15 mL) and the reaction stirred at RT for 24 hours. Water was added and the product extraxted into ether (2 x 100 mL). The combined organics were washed with brine, dried (MgSO4), filtered and reduced in vacuo to give a yellow oil which was chromatographed on silica, eluting with 0-40% ethyl acetate in isohexane, to give the desired material as a colourless oil (1.94 g). 1R NMR δ (CDCl3): -0.03 (s, 3H), 0.01 (s, 3H), 0.16 (s, 6H), 0.81 (s, 9H), 0.92 (s, 9H), 1.23 (d, 3H), 2.48 (s, 3H), 3.58 - 3.74 (m, 2H)5 4.37 - 4.42 (m, IH), 6.54 (t, IH), 6.86 (t, IH), 6.99 (t, IH), 8.06 (s, IH), 8.21 (s, IH), 9.48 (d, IH).
3-Hvdroxy-5-{ rd»Sf)-2-hvdroxy- 1 -methylethylioxyl-N-f 5-memylpyrazin-2-yl)benzamide
Figure imgf000231_0001
Trimethylsilyl iodide (6.06 mL, 42.75 mmol) was added to a solution of 3-hydroxy-5- {[(15)-l-methyl-2-(methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide (2.71 g, 8.55 mmol) in dry acetonitrile (150 mL) and stirred for 24 hours. Methanol (30 mL) was added to quench the reaction and stirred for 10 minutes. 10% w/v Aqueous sodium thiosulfate pentahydrate (20 mL) was added to the mixture and the organic solvents removed in vacuo. The residue was brought to pH5 with IM hydrochloric acid and ethyl acetate (80 mL) added. A yellow solid (1.4 g) was separated by filtration. The aqueous filtrate was reextracted into ethyl acetate (2x80 mL) and the combined organic layers dried (MgSO4), filtered and the solvents removed in vacuo. This residue was combined with the yellow solid obtained above and purified by column chromatography, eluting with 5% to 10% methanol in DCM, to give the title compound (1.70 g) 1H. ΝMR δ (de-DMSO): 1.21 (d, 3H), 2.50 (s, 3H), 3.40-3.60 (m, 2H), 4.45 (sex, IH), 4.80 (t, IH), 6.50 (s, IH), 6.97 (s, IH), 7.08 (s, IH), 8.32 (s, IH), 9.21 (s, 2H), 9.63 (s, IH), 10.80 (brs, IH). m/z 304 (M+H)+
The preparation of 3-hydroxy-5-{[(liS)-l-methyl-2-(methyloxy)ethyl]oxy}-N-(5- methylpyrazin-2-yl)benzamide was described earlier.
Example 59, 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{[(l1S)-2-hydroxy-l- methylethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide was also prepared in the following manner. A mixture of 3-hydroxy-5-{[(15)-2-hydroxy-l-methylethyl]oxy}-N-(5-methylpyrazin-2- yl)benzamide (3.03 g, 10.0 mmol), 2-(azetidin-l-ylcarbonyl)-5-chloropyrazine (2.17 g, 11.0 mmol) and potassium carbonate (4.14 g, 30.0 mmol) in acetonitrile (30.3 mL) was heated at 600C overnight. The solvent was removed by evaporation and the residue partitioned between ethyl acetate (15 vol) and water (10 vol). The aqueous phase was further extracted into ethyl acetate and the combined organics washed water, brine, dried (MgSO4), filtered and the solvent removed in vacuo. The residue was chromatographed on silica, eluting with 0.5-6% methanol in ethyl acetate, to give the desired material as a foam (3.1 g). A sample of this material (100 mg) was dissolved in the minimum volume of acetonitrile then isohexane (5 mL) added. The mixture was stirred for 3 days, the liquid decanted and the residue triturated with diethyl ether. The crystalline material was collected by filtration. 1H ΝMR δ (d6-DMSO): 1.25 (d, 3H), 2.30 (quin, 2H), 2.48 (s, 3H), 3.49 - 3.61 (m, 2H), 4.09 (t, 2H), 4.55 - 4.63 (m, 3H), 4.92 (t, IH), 7.12 (t, IH), 7.47 (t, IH), 7.57 (t, IH), 8.37 (d, IH), 8.58 (d, IH), 8.69 (d, IH), 9.26 (d, IH), 11.06 (s, IH); m/z 465 (M+H)+. Mpt (melting onset) 100.60C.
The title compound was also crystallised by stirring a slurry of the compound in ethyl acetate (melting point 125.O0C), toluene, nitromethane or methanol.
Example 60: 3-U5-(Azetidin-l-ylcarbonyl)pyrazin-2-yIloxy}-5-({(LS')-2- [(difluoromethyl)oxyl-l-methvIethyl|oxy)-iV-LH-pyrazol-3-ylbenzamide
Figure imgf000232_0001
Trifluoroacetic acid (2 mL) was added to a solution of 1,1-dimethylethyl 3-({[3-{[5- (azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-({(15)-2-[(difluoromethyl)oxy]-l- methylethyl}oxy)phenyl]carbonyl}amino)-lH-pyrazole-l-carboxylate (115 mg, 0.17 mmol) in DCM (16 mL) and stirred at RT for 2 hours. The solvent was removed in vacuo, DCM (20 mL) added and the mixture washed with water (20 mL), a saturated solution of sodium bicarbonate (20 mL), brine (20 mL), dried (MgSO4), filtered and reduced in vacuo to give the desired compound as a white foam (93 mg). 1H NMR δ (CDCl3): 1.36 (d, 3H), 2.37 (quin, 2H), 3.93 - 4.02 (m, 2H), 4.24 (t, 2H), 4.59 - 4.69 (m, 3H), 6.24 (t, IH), 6.82 (s, IH), 6.89 (t, IH), 7.29 - 7.31 (m, IH), 7.36 - 7.38 (m, IH), 7.40 (d, IH), 8.35 (d, IH), 8.79 (d, IH), 10.23 (s, IH), 10.48 (s, IH); m/z 489 (M+H)+
The preparation of 1,1-dimethylethyl 3-({[3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}~ 5-({ ( 1 »S)-2- [(difluoromethyl)oxy] - 1 -methylethyl } oxy)phenyl] carbonyl } amino)- IH- pyrazole-1-carboxylate is described below.
l.l-Dimethylethyl 3-r(r3-{r5-(azetidin-l-ylcarbonylWrazin-2-vnoxy}-5-α(l^-2- [f difluoromethyl)oxy~|- 1 -methylethyl ) oxy^phenyl] carbonyl } amino)- lH-pyrazole- 1 - carboxylate
Figure imgf000233_0001
l-Chloro-N,N,2-trimethyl-l-propenylamine (0.12 mL, 0.92 mmol) was added to a solution of 3 - { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl] oxy }-5-({(l S)-2- [(difluoromethyl)oxy] - 1 - methylethyl} oxy)benzoic acid (0.26 g, 0.61 mmol) in DCM (8 mL) and stirred for 1 hour. 1,1-Dimethylethyl 3-amino-lH-pyrazole-l -carboxylate (169 mg, 0.92 mmol) then pyridine (0.1 mL, 1.23 mmol) were added and stirred for a further 20 hours. The reaction mixture was reduced in vacuo and ethyl acetate (50 mL) and water (50 mL) were added. The aqueous layer was re-extracted into ethyl acetate (50 mL) and the combined organics washed with water (50 mL), brine (50 mL), dried (MgSO4), filtered and reduced in vacuo. The crude oil was chromatographed on silica, eluting with 25-50% ethyl acetate in isohexane, to give the desired compound as a colourless oil (100 mg). 1H ΝMR δ (CDCl3): 1.38 (d, 3H), 1.62 (s, 9H), 2.38 (quin, 2H), 3.93 - 4.04 (m, 2H), 4.26 (t, 2H), 4.61 - 4.65 (m, IH), 4.69 (t, 2H), 6.26 (t, IH), 6.95 (t, IH), 7.08 (d, IH), 7.25 - 7.26 (m, IH), 7.33 (t, IH)5 8.01 (d, IH), 8.34 (d, IH), 8.84 (s, IH), 8.85 (d, IH); m/z 587 (M-H)- The preparation of 1,1-dimethylethyl 3-amino-lH-pyrazole-l-carboxylate was described earlier.
The preparation of 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-({(15)-2- [(difluoromethyl)oxy]-l-methylethyl}oxy)benzoic acid is described below.
3 - { \5 -CAzetidin- 1 -ylcarbonyr)pyrazin-2-vπ oxyl -5-( (Cl >SV2-r(difluoromethyr)oxy1 - 1 - methylethyl}oxy)benzoic acid
Figure imgf000234_0001
Lithium hydroxide monohydrate (48 mg, 1.13 mmol) in water (5 mL) was added to a solution of methyl 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-({(15)-2-
[(difluoromethyl)oxy]-l-methylethyl}oxy)benzoate (0.33 g, 0.75 mmol) in TΗF (10 mL) and stirred at RT for 20 hours. The TΗF was removed in vacuo and the aqueous layer washed with ethyl acetate (50 mL) to remove any impurities. The aqueous layer was acidified and extracted into ethyl acetate (2 x 50 mL), washed with brine, dried (MgSO4), filtered and the solvent removed in vacuo to give the desired compound as a white foam (0.26 g). m/z 424 (M+Η)+
Methyl 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxyl-5-('l(l^-2-[(difluoromethyl)oxyl- 1 -methylethyl } oxy)benzoate
Figure imgf000234_0002
A mixture of methyl 3-({(lS)-2-[(difluoromethyl)oxy]-l-methylethyl}oxy)-5- hydroxybenzoate (0.28 g, 1.01 mmol), 2-(azetidin-l-ylcarbonyl)-5-chloropyrazine (201 mg, 1.01 mmol) and potassium carbonate (280 mg, 2.03 mmol) in acetonitrile (5 mL) was stirred in a microwave reactor at 140°C for 5 hourrs. The reaction mixture was reduced in vacuo, ethyl acetate (50 mL) added and the organics washed with water (50 mL), brine (50 mL), dried (MgSO4), filtered and reduced in vacuo. The crude oil was chromatographed on silica, eluting with 20 to 70% ethyl acetate in isohexane, to give the desired compound as a colourless oil (330 mg). 1H NMR δ (CDCl3): 1.38 (d, 3H), 2.37 (quin, 2H), 3.90 (s, 3H), 3.93 - 4.04 (m, 2H), 4.26 (d, 2H), 4.61 - 4.70 (m, 3H), 6.25 (t, IH), 6.95 (t, IH), 7.43 - 7.44 (m, IH), 7.49 - 7.50 (m, IH), 8.32 (d, IH), 8.85 (d, IH); m/z 438 (M+H)+
Methyl 3-({(lS)-2- [CdifluoromethyDoxy]- 1 -methylethyl } oxyV 5-hydroxybenzoate
Figure imgf000235_0001
Methyl 3-({(lS)-2-[(difluoromethyl)oxy]-l-methylethyl}oxy)-5-
[(phenylmethyl)oxy]benzoate (0.48 g, 1.08 mmol) was dissolved in ethanol (10 mL) and THF (10 mL) and the flask evacuated and purged with argon (3 times). 10% Palladium on carbon (140 mg) was added and the flask further evacuated and finally purged with hydrogen gas. The reaction mixture was stirred at RT for 20 hours until completion. The reaction mixture was evacuated and purged with argon (3 times) then the catalyst removed by filtration through celite®. The filtrate was concentrated in vacuo to give the desired compound as a colourless oil (1.05 g). 1H NMR δ (CDCl3): 1.35 (d, 3H), 3.90 (s, 3H), 3.90 - 4.02 (m, 2H), 4.57 - 4.64 (m, IH), 5.20 (s, IH), 6.26 (t, IH), 6.63 (t, IH), 7.14 - 7.15 (m, IH), 7.17 - 7.18 (m, IH); m/z 275 (M-H)" The preparations of methyl 3-({(lS)-2-[(difluoromethyl)oxy]-l-methylethyl}oxy)-5- [(phenylmethyl)oxy]benzoate and 2-(azetidin-l-ylcarbonyl)-5-chloropyrazine were described earlier.
Example 61 : 3-1 f 5-(Azetidin-l-vIsulfonyl)-4-methyl-l,3-thiazoI-2-vIl oxy}-5-{ UlS)-I- methyI-2-(methvIoxy)ethvnoxyl-iV-(5-methylpyrazin-2-yl)benzamide
Figure imgf000235_0002
Cesium carbonate (293 mg, 0.9 mmol) was added to a solution of 3-hydroxy-5-{[(15)-l- methyl-2-(methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide (95 mg, 0.3 mmol) and 5-(azetidin-l-ylsulfonyl)-2-chloro-4-methyl-l,3-thiazole (90 mg, 0.36 mmol) in acetonitrile (5 mL) and the stirred mixture heated in a microwave reactor at 12O0C for 1 hour. The mixture was cooled to RT and pressure, the acetonitrile removed in vacuo, and the residue partitioned between water (15 mL) and ethyl acetate (30 mL). The organic layer was washed with brine, dried (MgSO4) and evaporated in vacuo to a residue which was chromatographed on silica, eluting with 60% ethyl acetate in isohexane, to give the desired material as a colourless solid (79 mg). 1H ΝMR δ (CDCl3): 1.3 (d, 3H), 2.15 (m, 2H), 2.5 (s, 3H), 2.5 (s, 3H), 3.35 (s, 3H), 3.45 - 3.55 (m, 2H), 3.85 (t, 4H), 4.55 (m, IH), 7.0 (d, IH), 7.35 (d, 2H), 8.05 (s, IH), 8.3 (s, IH), 9.45 (s, IH); m/z 534 (M+H)+
The preparation of 3-hydroxy-5-{[(l»S)-l-methyl-2-(methyloxy)ethyl]oxy}-N-(5- methylpyrazin-2-yl)benzamide was described earlier.
The preparation of 5-(azetidin-l-ylsulfonyl)-2-chloro-4-methyl-l,3-thiazole is described below.
5-(Azetidin- 1 -ylsulfonyl)-2-chloro-4-methyl- 1 ,3-thiazole o&-* <V T
A solution of 2-chloro-4-methylthiazole-5-sulphonylchloride (462 mg; 2.0 mmol) in DCM (5 mL) was added to an ice-cold solution of azetidine hydrochloride (196 mg; 2.1 mmol) and triethylamine (1.0 mL; 7.2 mmol) in DCM (15 mL) and the mixture stirred at O0C for 30 minutes. The DCM was removed in vacuo to give a residue which was partitioned between water (50 mL) and ethyl acetate (75 mL). The organic layer was washed with brine, dried (MgSO4) and evaporated to a residue which was chromatographed on silica, eluting with 10% ethyl acetate in isohexane, to give the desired material as a solid which was crystallised from ethyl acetate and isohexane (100 mg). 1H NMR δ (CDCl3): 2.2 (dt, 2H), 2.65 (s, 3H), 3.9 (t, 4H); m/z 253 (M+H)+ Example 62: 3-{[6-rAzetidin-l-ylcarbonvI)pyridin-3-ylloxy}-iV-fl-(difluoroniethvIV lJ3-PyrazoI-3-vn-5-f(35)-tetrahvdrofuran-3-yIoxylbenzamide
Figure imgf000237_0001
l-Chloro-N,N,2-trimethyl-l-propenylamine (0.088 mL, 0.66 mmol) was added to a solution of 3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-[(3.S)-tetraliydroftιran-3- yloxy]benzoic acid (171 mg, 0.44 mmol) in DCM (10 mL) and the reaction was stirred at RT for 30minutes. After this time l-(difluoromethyl)pyrazol-3 -amine hydrochloride (75 mg, 0.44 mmol) and DIPEA (0.154 mL, 0.89 mmol) were added sequentially. The resulting solution was stirred at RT for 16 hours after which time the solvent was removed in vacuo and ethyl acetate (20 mL) and water (20 mL) were added. The aqueous phase was extracted with ethyl acetate (2 x20 mL), washed with a saturated solution of sodium hydrogen carbonate (20 mL), and brine (20 mL). The combined organic extracts were dried (MgSO4), filtered and evaporated. The residue was purified by flash column chromatography on silica, eluting with 50-100% ethyl acetate in isohexane, to give the desired product as a white solid (132 mg). 1H ΝMR δ (CDCl3): 2.40 - 2.12 (m, 4H), 4.03 - 3.89 (m, 4H), 4.25 (t, 2H), 4.71 (t, 2H), 5.01 - 4.96 (m, IH), 6.76 (t, IH), 7.03 (t, IH), 7.08 (d, IH), 7.11 (s, IH), 7.22 (s, IH), 7.38 (dd, IH), 7.75 (d, IH)3 8.13 (d, IH), 8.33 (d, IH), 8.50 (s, IH); 19F ΝMR δ (CDCl3): -93.84; m/z 500 (M+H)+.
The following compounds were made in an analogous fashion.
Figure imgf000237_0002
Figure imgf000238_0002
The preparations of 3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3-yl]oxy } -5 - [(3S)- tetrahydrofuran-3 -yloxy]benzoic acid, 3 - { [5 -(azetidin- 1 -ylcarbonyl)pyrazin-2-yl] oxy } -5- {[(lS)-l-methyl-2-(methyloxy)ethyl]oxy}benzoic acid and 3-{[6-(azetidin-l- ylcarbonyl)pyridin-3-yl]oxy}-5-{[(lS)-l-methyl-2-(methyloxy)ethyl]oxy}benzoic acid were described earlier.
The preparation of l-(difluoromethyl)pyrazol-3 -amine is described in the literature [WO2005090332, PCT Int. Appl. (2005)].
Example 63: 3-{[5-(Azetϊdin-l-ylcarbonyl)pyrazin-2-ylloxy}-5-{[(lig)-l-methvI-2- (methyloxy)ethylloxy}-N-(5-methvIpyrazin-2-yr)benzamide
Figure imgf000238_0001
l-Chloro-N,N,2-trimethyl-l-propenylamine (0.042 mL, 0.31 mmol) was added to a solution of 3- { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy }-5- { [(Ii?)- 1 -methyl-2- (methyloxy)ethyl]oxy}benzoic acid (0.102 g, 0.0.31 mmol) in DCM (3 mL) under argon and stirred at RT for 40 minutes. 2-Amino-5-methylpyrazine (57 mg, 0.52 mmol) and pyridine (0.043 mL, 0.52 mmol) were added and the reaction stirred for a further 3 hours. The solvent was removed in vacuo and the residue taken up in ethyl acetate (30 mL), washed with water (2 x 10 mL), a saturated aqueous solution of sodium bicarbonate (10 mL), brine (10 mL), dried (MgSO4), filtered, and evaporated in vacuo. The crude material was chromatographed on silica, eluting with a gradient of 60-100% ethyl acetate in isohexane, to give the desired compound as a white foam (86 mg). 1H NMR δ (CDCl3): 1.35 (d, 3H), 2.32 - 2.44 (m, 2H), 2.56 (s, 3H), 3.41 (s, 3H), 3.47 - 3.64 (m, 2H), 4.26 (t, 2H), 4.62 (q, IH), 4.69 (t, 2H), 6.98 (s, IH), 7.29 (s, IH), 7.41 (s, IH), 8.13 (s, IH), 8.35 (s, 2H), 8.86 (s, IH), 9.54 (s, IH); m/z 479 (M+H)+
The preparation of 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{[(li?)-l-methyl-2- (methyloxy)ethyl]oxy}benzoic acid is described below.
3-([5-rAzetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-ir('li?Vl-methyl-2- (metfayloxy'jethylloxylbenzoic acid
Figure imgf000239_0001
Methyl 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{[(lJR)-l-methyl-2-
(methyloxy)ethyl]oxy}benzoate (1.06 g, 2.6 mmol) was dissolved in THF (24 niL) and methanol (8 mL) then IN lithium hydroxide solution (3.12 mL) added, followed by the dropwise addition of water (10 mL). The resultant solution was stirred for 3 hours at RT, a further portion of IN lithium hydroxide solution (1.3 mL), the reaction stirred a further 2 hours, then further IN lithium hydroxide solution (0.6 mL) added and stirring continued for another hour. The organics were removed by evaporation under reduced pressure, the aqueous solution was washed with diethyl ether (10 mL) then acidified with 2N hydrochloric acid and extracted with ethyl acetate (3 x 20 mL). The combined organics were washed with water (10 mL), brine (10 mL) and evaporated to dryness under reduced pressure to give the desired compound as a white solid (887 mg).
1H NMR δ (CDCl3): 1.34 (d, 3H), 2.38 (quin, 2H), 3.41 (s, 3H), 3.47 - 3.64 (m, 2H), 4.27 (t, 2H), 4.55 - 4.65 (m, IH), 4.69 (t, 2H), 6.99 (s, IH), 7.45 (s, IH), 7.55 (s, IH), 8.33 (s, IH), 8.84 (s, IH); m/z 388 (M+H)+ Methyl 3-1 r5-Cazetidin- 1 -ylcarbonvDpyrazin-2-vnoxy> -5- { \( IK)- 1 -methyl-2-
(methyloxy)ethvHoxyibenzoate
Figure imgf000240_0001
A mixture of methyl 3-hydroxy-5-{[(li?)-l-methyl-2-(methyloxy)ethyl]oxy}benzoate (727 mg, 3 mmol), 2-(azetidin-l-ylcarbonyl)-5-chloropyrazine (712 mg, 3.6 mmol) and potassium carbonate (828 mg, 6 mmol) in DMA (10 mL) was stirred at 120°C for 2 hours. The solution was diluted with ethyl acetate(150 mL) washed with water (3 x 50 mL), brine (20 mL), dried (MgSO4), filtered and the solvent removed in vacuo. The residue was purified by chromatography on silica, eluting with 50% ethyl acetate in isohexane, to give the desired material as a colourless oil (1.07 g). 1H NMR δ (CDCl3): 1.27 (d, 3H), 2.4 (quintet, 2H), 3.4(s, 3H), 3.5 - 3.6 (m, 2H), 3.9 (s, 3H), 4.25 (t, 2H), 4.53 (q, IH), 4.7 (t, 2H), 6.95 (s, IH), 7.4 (s, IH), 7.5 (s, IH), 8.3(s, IH), 8.85 (s, IH); m/z 402 (M+H)+
The preparation of 2-(azetidin-l-ylcarbonyl)-5-chloropyrazine was described earlier.
Methyl 3-hvdroxy-5-{[fliO-l-methyl-2-(methyloxy)ethyl'|oxy}benzoate
Figure imgf000240_0002
A solution of methyl 3-{[(li?)-l-methyl-2-(methyloxy)ethyl]oxy}-5- [(phenylmethyl)oxy]benzoate in THF (5 mL) and ethanol (5 mL), containing 10% palladium on carbon, was stirred under an atmosphere of hydrogen for 16 hours. The palladium on carbon was removed by filtration and the filtrate evaporated under reduced pressure to give the desired material as a clear gum (723 mg).
1H NMR δ (CDCl3): 1.31 (d, 3H), 3.42 (s, 3H), 3.48 - 3.64 (m, 2H), 3.88 (s, 3H), 4.54 - 4.63 (m, IH), 5.75 (s, IH), 6.64 (s, IH), 7.11 (s, IH), 7.17 (s, IH); m/z 241 (M+H)+ Methyl 3-{[rii?Vl-methyl-2-rmethyloxy)ethyl'|oxyi-5-frphenylmethyl)oxy]benzoate
Figure imgf000241_0001
DIAD (4.6 g, 0.029mol) was added dropwise to a solution of methyl 3-hydroxy-5- {[phenylmethyl]oxy}benzoate (6 g, 0.023 mol), (S)-(H-)- l-methoxy-2-propanol (2.59 g, 0.029 mol) and triphenylphosphine (7.53 g, 0.029 mol) in THF (100 mL), under argon, at 0°C. The reaction was stirred at O0C for 1 hour and at RT for 20 hours. The volatiles were removed in vacuo and isohexane / ethyl acetate 2:1 added followed by stirring for 1 hour. A white solid was removed by filtration and the filtrate was evaporated to a residue which was chromatographed on silica, eluting with a gradient of 0-20% ethyl acetate in isohexane, to give the desired compound (5.11 g).
1H NMR δ (CDCl3): 1.31 (d, 3H), 3.40 (s, 3H), 3.45-3.60 (m, 2H), 3.88 (s, 3H), 4.57 (sex, IH), 5.07 (s, 2H), 6.76 (m, IH), 7.25 (m, 2H), 7.40 (m, 5H). m/z 331 (M+H)+.
The preparation of methyl 3-hydroxy-5-{[phenylmethyl]oxy}benzoate was described earlier.
Example 64: 3-{r6-(Azetidin-l-vIcarbonyl)pyridin-3-ylloxyi-5-{rfli?)-l-methvI-2- (methvIoxy)ethylloxy}-iV-(5-methvIpyrazin-2-yl)benzamide
Figure imgf000241_0002
A mixture of 3-{[6-(azetidin-l-ylcarbonyl)ρyridin-3-yl]oxy}-5-hydroxy-N-(5- methylρyrazin-2-yl)benzamide (101 mg, 0.25 mmol), S-(+)-methoxy-2-propanol (0.049 mL, 0.50 mmol) and triphenyl phosphine (131 mg, 0.50 mmol) in dry THF (10 mL) was treated dropwise with DIAD (0.099 mL, 0.50 mmol) at O0C under an argon atmosphere. The mixture was allowed to warm to RT and stirred overnight. The THF was removed by evaporation under reduced pressure and the residue purified twice by chromatography on silica, eluting with 0-7% methanol in DCM. The desired fractions were combined and purified by elution through an SCX column, eluted with 5% methanol in DCM then 50% 7N ammonia (in methanol) in DCM. The desired fractions were purified by elution though an NH2 column, eluting with 5% methanol in DCM, to give the desired compound (35 mg) with a purity estimated to be 85%. 1H NMR δ (CDCl3): 1.26 (d, 3H), 2.28 (quin, 2H), 2.49 (s, 3H), 3.33 (s, 3H), 3.41 - 3.54 (m, 2H), 4.18 (t, 2H), 4.50 - 4.58 (m, IH), 4.64 (t, 2H), 6.77 (s, IH), 7.08 (s, IH), 7.25 (s, IH), 7.31 (d, IH), 8.04 (d, IH), 8.07 (s, IH), 8.26 (d, IH), 8.30 (s, IH), 9.46 (s, IH); m/z 478 (M+H)+
The preparation of 3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-hydroxy-N-(5- methylpyrazin-2-yl)benzamide is described below.
3-1 f 6-fAzetidin- 1 - ylcarbonyl)pyridin-3 -yl] oxy I -5-hydroxy-JV-(5-methylpyrazin-2- vDbenzamide
Figure imgf000242_0001
3-{[6-(Azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-N-(5-methylpyrazin-2-yl)-5- [(phenylrnethyl)oxy]benzamide (3.9 g, 7.9 mmol) was dissolved in ethyl acetate (200 mL) and ethanol (200 mL) then 10% palladium on charcoal added. The reaction was stirred under an atmosphere of hydrogen for 16 hours. Methanol (150 mL) was added to aid solubilisation of the material and the suspension filtered, the residue taken up in DMF, filtered and the combined filtrates evaporated to dryness to give the desired material (3.17 g). 1H ΝMR δ (d6-DMSO): 2.33 (quin, 2H), 2.53 (s, 3H), 4.13 (t, 2H), 4.64 (t, 2H), 6.81 (s, IH), 7.31 (s, IH), 7.34 (s, IH), 7.62 (d, IH), 8.05 (d, IH), 8.40 (s, IH), 8.47 (s, IH), 9.26 (s, IH), 10.35 (s, IH), 11.02 (s, IH); m/z 406 (M+H)+ 3 - { F6-f Azetidin- 1 -ylcarbonvDpyridin-3 -yl] oxy } -N-("5-methylpyrazin-2-ylV5- FfphenylmethvDoxylbenzamide
Figure imgf000243_0001
l-Chloro-N,N,2-trimethyl-l-propenylamine (2.67 mL, 20 mmol) was added to a solution of 3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-[(phenylmethyl)oxy]benzoic acid (6.18 g, 15.3 mmol) in DCM (100 mL) and the reaction stirred at RT for 50mins. 2-Amino-5- methylpyrazine (3.34 g, 30.6 mmol) and pyridine (2.5 mL, 30.6 mmol) were added and the reaction stirred overnight. The solvent was removed in vacuo, and the residue taken up in ethyl acetate(350 mL). The organic phase was washed with water (2 x 100 mL), brine (100 mL), dried (MgSO4), filtered, and evaporated in vacuo. The residue was chromatographed on silica, eluting with a gradient of 50-75% ethyl acetate in isohexane, to give the desired material (4.01 g). 1H ΝMR δ (CDCl3): 2.28 (quin, 2H), 2.49 (s, 3H), 4.18 (t, 2H), 4.63 (t, 2H), 5.05 (s, 2H), 6.78 (s, IH), 7.10 (s, IH), 7.25 - 7.37 (m, 7H), 8.04 (d, IH), 8.07 (s, IH), 8.25 (s, 2H), 9.46 (s, IH); m/z 496 (M+H)+
3-( r6-(Azetidin- 1 -ylcarbonyl)pyridin-3 -yll oxy I -5- [(phenylmethypoxylbenzoic acid
Figure imgf000243_0002
A mixture of methyl 3-hydroxy-5-{[phenylmethyl]oxy}benzoate (5.16 g, 20 mmol), 2- (azetidin-l-ylcarbonyl)-5-bromopyridine (5.3 g, 22 mmol), cesium carbonate (19.56 g, 60 mmol) and bromotris(triphenylphosphine)copper (3.73 g, 4 mmol) in DMA (100 mL) was stirred at 160°C for 6 hours in a microwave reactor. The DMA was evaporated under reduced pressure and the residue was dissolved in water (200 mL) and washed with ethyl actate (3 x 50 mL). The aqueous layer was acidified with 2Ν hydrochloric acid and extracted with ethyl acetate (3 x 100 mL), the combined organics were washed with water (2 x 20 mL), brine (20 mL), dried (MgSO4), filtered and the solvent removed in vacuo to give the desired material (6.18 g). 1H NMR δ (CDCl3): 2.29 (s, 2H), 4.20 (s, 2H), 4.64 (s, 2H), 5.04 (s, 2H), 6.83 (s, IH), 7.22 - 7.44 (m, 7H), 7.49 (s, IH), 7.79 - 8.63 (m, 2H); m/z 405 (M+H)+
The preparations of methyl 3-hydroxy-5-{[phenylrnethyl]oxy}benzoate and 2-(azetidin-l- ylcarbonyl)-5-bromopyridine were described earlier.
BIOLOGICAL
Tests:
The biological effects of the compounds of formula (I) may be tested in the following way:
(1) Enzymatic activity
Enzymatic activity of recombinant human pancreatic GLK may be measured by incubating GLK, ATP and glucose. The rate of product formation may be determined by coupling the assay to a G-6-P dehydrogenase, NADP/NADPH system and measuring the linear increase with time of optical density at 340nm (Matschinsky et al 1993). Activation of GLK by compounds can be assessed using this assay in the presence or absence of GLKRP as described in Brocklehurst et al (Diabetes 2004, 53, 535-541).
Production of recombinant GLK and GLKRP:
Human GLK and GLKRP cDNA was obtained by PCR from human pancreatic and hepatic mRNA respectively, using established techniques described in Sambrook J, Fritsch EF & Maniatis T, 1989. PCR primers were designed according to the GLK and GLKRP cDNA sequences shown in Tanizawa et al 1991 and Bonthron, D.T. et al 1994 (later corrected in Warner, J.P. 1995).
Cloning in Bluescript II vectors
GLK and GLKRP cDNA was cloned in E. coli using pBluescript II, (Short et al 1998) a recombinant cloning vector system similar to that employed by Yanisch-Perron C et al (1985), comprising a colEI-based replicon bearing a polylinker DNA fragment containing multiple unique restriction sites, flanked by bacteriophage T3 and T7 promoter sequences; a filamentous phage origin of replication and an ampicillin drug resistance marker gene.
Transformations
E. Coli transformations were generally carried out by electroporation. 400 mL cultures of strains DH5a or BL21(DE3) were grown in L-broth to an OD 600 of 0.5 and harvested by centrifugation at 2,00Og. The cells were washed twice in ice-cold deionised water, resuspended in ImL 10% glycerol and stored in aliquots at -700C. Ligation mixes were desalted using Millipore V series™ membranes (0.0025mm) pore size). 4OmL of cells were incubated with ImL of ligation mix or plasmid DNA on ice for 10 minutes in 0.2cm electroporation cuvettes, and then pulsed using a Gene Pulser™ apparatus (BioRad) at O.SkVcm"1, 25OmF. Transformants were selected on L-agar supplemented with tetracyline at lOmg/mL or ampicillin at lOOmg/mL.
Expression
GLK was expressed from the vector pTB375NBSE in E.coli BL21 cells,, producing a recombinant protein containing a 6-His tag immediately adjacent to the N-terminal methionine. Alternatively, another suitable vector is pET21(+)DNA, Novagen, Cat number 697703. The 6-His tag was used to allow purification of the recombinant protein on a column packed with nickel-nitrilotriacetic acid agarose purchased from Qiagen (cat no
30250).
GLKRP was expressed from the vector pFLAG CTC (IBI Kodak) in E.coli BL21 cells, producing a recombinant protein containing a C-terminal FLAG tag. The protein was purified initially by DEAE Sepharose ion exchange followed by utilisation of the
FLAG tag for final purification on an M2 anti-FLAG immunoaffϊnity column purchased from Sigma-Aldrich (cat no. A1205).
(2) Oral Glucose Tolerance Test fOGTT)
Oral glucose tolerance tests were done on conscious Zucker obese fa/fa rats (age 12-13 weeks or older) fed a high fat diet (45 % kcal fat) for at least two weeks prior to experimentation. The animals were fasted for 2 hours before use for experiments. A test compound or a vehicle was given orally 120 minutes before oral administration of a glucose solution at a dose of 2 g/kg body weight. Blood glucose levels were measured using a Accucheck glucometer from tail bled samples taken at different time points before and after administration of glucose (time course of 60 minutes). A time curve of the blood glucose levels was generated and the area-under-the-curve (AUC) for 120 minutes was calculated (the time of glucose administration being time zero). Percent reduction in glucose excursion was determined using the AUC in the vehicle-control group as zero percent reduction. Compounds of the invention generally have an activating activity for glucokinase with an EC50 of less than about lμM and generally less than about 50OnM. For example, Example 3 has an EC50 of 31nm. Example 3 exhibits 54% OGTT activity at 10mg/kg.
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Claims

Claims:
1. A compound of Formula (I):
Figure imgf000250_0001
(I) wherein:
R1 is selected from isopropyl, but-2-yl, cyclopentyl, l,l,l-trifluoroprop-2-yl, 1,3- difluoroprop-2-yl, but-l-yn-3-yl, l-hydroxyprop-2-yl, 2-hydroxybut-3-yl, l-hydroxybut-2- yl, tetrahydrofuryl, tetrahydropyranyl, l-methoxyprop-2-yl, l-methoxybut-2-yl, 2- hydroxyprop-1-yl, 2-methoxyprop-l-yl, 2-hydroxybut-l-yl, 2-methoxybut-l-yl, 1- fluoromethoxyprop-2-yl, l,l-difluoromethoxyprop-2-yl and l-trifluoromethoxyprop-2-yl; HET-I is a 5- or 6-membered, C-linked heteroaryl ring containing a nitrogen atom in the 2- position and optionally 1 or 2 further ring heteroatoms independently selected from O, N and S; which ring is optionally substituted on any nitrogen atom (provided it is not thereby quaternised) by a substituent selected from R7 and/or on 1 or 2 available carbon atoms by a substituent independently selected from R6;
HET-2 is a 5- or 6- membered heteroaryl ring, containing 1, 2 or 3 ring hetereoatoms independently selected from O, S and N; which ring is substituted on an available carbon atom by a substituent selected from R2, and is optionally further substituted on 1 or 2 available carbon atoms by a substituent independently selected from R3 and/or on an available nitrogen atom (provided it is not thereby quaternised) by a substituent selected from R10;
R2 is selected from -C(O)NR4R5 and -SO2NR4R5;
R3 is selected from methyl, trifluoromethyl and halo; R4 and R5 together with the nitrogen atom to which they are attached form a 4 to 7 membered saturated or partially unsaturated heterocyclyl ring, optionally containing 1 or 2 further heteroatoms (in addition to the linking N atom) independently selected from O, N and S, wherein a -CH2- group can optionally be replaced by a -C(O)- and wherein a sulphur atom in the ring may optionally be oxidised to a S(O) or S(O)2 group; which ring is optionally substituted on an available carbon atom by 1 or 2 substituents independently selected from R8 and/or on an available nitrogen atom by a substituent selected from R9; or R4 and R5 together with the nitrogen atom to which they are attached form a 6-10 membered bicyclic saturated or partially unsaturated heterocyclyl ring, optionally containing 1 further nitrogen atom (in addition to the linking N atom), wherein a -CH2- group can optionally be replaced by a -C(O)-; which ring is optionally substituted on an available carbon by 1 substituent selected from hydroxy and R3 or on an available nitrogen atom by methyl;
R6 is independently selected from (l-4C)alkyl, halo, hydroxy(l-4C)alkyl, (l-4C)alkoxy(l- 4C)alkyl, (l-4C)alkylS(O)p(l-4C)alkyl, amino(l-4C)alkyl, (l-4C)alkylamino(l-4C)alkyl and di(l-4C)alkylamino(l-4C)alkyl; R7 is independently selected from (l-4C)alkyl, halo(l-4C)alkyl, dihalo(l-4C)alkyl, trihalo(l-4C)alkyl, hydroxy(l-4C)alkyl, (l-4C)alkoxy(l-4C)alkyl, (l-4C)alkylS(0)p(l- 4C)alkyl, amino(l-4C)alkyl, (l-4C)alkylamino(l-4C)alkyl and di(l-4C)alkylamino(l- 4C)alkyl;
R8 is selected from hydroxy, (l-4C)alkoxy, (l-4C)alkyl, aminocarbonyl, (1- 4C)alkylaminocarbonyl, di(l-4C)alkylaminocarbonyl, (l-4C)alkylamino, di(l- 4C)alkylamino, (l-4C)alkoxy(l-4C)alkyl, hydroxy(l-4C)alkyl and -S(0)p(l-4C)alkyl; R9 is selected from (l-4C)alkyl, -C(O)(l-4C)alkyl, aminocarbonyl, (1- 4C)alkylaminocarbonyl, di( 1 -4C)alkylaminocarbonyl, ( 1 -4C)alkoxy( 1 -4C)alkyl, hydroxy(l-4C)alkyl and -S(0)p(l-4C)alkyl; R10 is selected from (l-4C)alkyl, (3-6C)cycloalkyl, hydroxy(l-4C)alkyl, (l-4C)alkoxy(l- 4C)alkyl, -C(O)(I -4C)alkyl, benzyl, and (l-4C)alkylsulfonyl; p is (independently at each occurrence) 0, 1 or 2; or a salt thereof.
2. A compound of the formula (I) as claimed in Claim 1 or a salt thereof wherein R1 is of sub-formula X:
Figure imgf000252_0001
(X) wherein Rx is selected from methyl, ethyl, trifluoromethyl, ethynyl, hydroxymethyl, hydroxyethyl, methoxymethyl, fluoromethoxymethyl, difluoromethoxymethyl and trifluoromethoxymethyl.
3. A compound of the formula (I) as claimed in Claim 1 or Claim 2 or a salt thereof, wherein R2 is -CONR4R5.
4. A compound of the formula (I) as claimed in Claim 1 or a salt thereof, wherein: R1 is selected from isopropyl, cyclopentyl, l,3-difluoroprop-2-yl, but-2-yl, 1- hydroxyprop-2-yl, 2-hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, tetrahydropyranyl, l-hydroxybut-2-yl and l-methoxyprop-2-yl;
HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl, pyridyl, isoxazolyl and pyrazinyl, wherein R1 is optionally substituted with a methyl, isopropyl or ethyl group and/or (on a carbon atom) by fluoro;
HET-2 is selected from pyridyl, pyrazinyl, pyridazinyl, thiazolyl and pyrimidinyl, substituted by R2 and optionally substituted by R3;
R3 is methyl, fluoro or chloro;
R2 is selected from -C(O)NR4R5 and -SO2NR4R5;
R4 and R5 together form an azetidinyl, 7-azabicyclo[2.2.1]hept-7-yl, morpholino, or pyrrolidinyl ring.
5. A compound of the formula (I) as claimed in Claim 1 or a salt thereof, wherein: R1 is selected from isopropyl, cyclopentyl, l,3-difluoroprop-2-yl, but-2-yl, 1- hydroxyprop-2-yl, 2-hydroxybut-3-yl, l-difluoromethoxyprop-2-yl, tetrahydrofuryl, tetrahydropyranyl, l-hydroxybut-2-yl and l-methoxyprop-2-yl; HET-I is selected from thiazolyl, pyrazolyl, thiadiazolyl, pyridyl, isoxazolyl and pyrazinyl, wherein R1 is optionally substituted with a methyl, isopropyl or ethyl group and/or (on a carbon atom) by fluoro;
HET-2 is selected from pyridyl, pyrazinyl, pyridazinyl, thiazolyl and pyrimidinyl, substituted by R2 and optionally substituted by R3; R3 is methyl, fluoro or chloro; R2 is -CONR4R5; R4 and R5 together form an azetidinyl or pyrrolidinyl ring.
6. A compound of the formula (I) as claimed in any one of Claims 1 to 5, or a salt thereof, wherein R4 and R5 together form an azetidinyl ring.
7. A compound of the formula (I) as claimed in any one of Claims 1 to 6 which is a compound of formula (IA) or a salt thereof:
Figure imgf000253_0001
(IA) wherein each of X1, X2 and X3 is independently selected from CH, N, S and O; X4 is absent (to make a 5-membered ring) or is selected from CH, N, O and S; provided that at least one of X1, X2, X3 and X4 is CH and provided that there are no 0-0, O-S or S-S bonds within the ring.
8. A compound of the formula (I) as claimed in Claim 1 which is any one or more of the following: 3-{[5-(azetidin-l-ylcarbonyl)pyridin-2-yl]oxy}-5-{[(lS)-2-hydroxy-l-methylethyl]oxy}- N-l,3-thiazol-2-ylbenzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl]oxy } -5- { [( 1 S)-2-hydroxy- 1 - methylethyl]oxy}-N- 1 ,3-thiazol-2-ylbenzamide; 3- { [6-(azetidin- 1 -ylcarbonyl)pyridin-3-yl]oxy } -5- { [(I S)-2-hydroxy- 1 -methylethyl]oxy } -
N- 1 ,3-thiazol-2-ylbenzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)pyridin-3 -yl]oxy } -5 - { [( 1 S)-2-hydroxy- 1 -methylethyl] oxy } -
N-(I -methyl- 1 H-pyrazol-3 -yl)benzamide; 3-{ [6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-{ [(I S)-2-hydroxy-l-methylethyl]oxy}-
N-( 1 -methyl- 1 H-pyrazol-3-yl)benzamide;
3- { [5-(azetidin- 1 -ylcarbonyl)pyridin-2-yl]oxy }-5- { [( 1 S)- 1 -methyl-2-
(methyloxy)ethyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl]oxy } -5- { [( 1 S)- 1 -methyl-2- (methyloxy)ethyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)pyridin-2-yl] oxy } -5-[( 1 -methylethyl)oxy] -N- 1 ,3 -thiazol-2- ylbenzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl] oxy } -5 -[( 1 -methylethyl)oxy] -N- 1,3- thiazol-2-ylbenzamide; 3-{[5-(azetidin-l-ylcarbonyl)pyridin-2-yl]oxy}-5-[(l-methylethyl)oxy]-N-(l-methyl-lH- pyrazol-3 -yl)benzamide ;
3 - { [5 -(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl] oxy } -5-[( 1 -methylethyl)oxy]-N-( 1 - methyl-lH-pyrazol-3-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyridin-2-yl]oxy}-5-{[(lS)-l-methyl-2- (methyloxy)ethyl]oxy}-N-(3-methyl-l,2,4-thiadiazol-5-yl)benzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl] oxy } -5- { [( 1 S)- 1 -methyl-2-
(methyloxy)ethyl]oxy}-N-(3-methyl-l,2,4-thiadiazol-5-yl)benzamide;
3-{ [6-(azetidin- 1 -ylcarbonyl)pyridin-3-yl]oxy}-5-{ [(I S)-I -methyl-2-
(methyloxy)ethyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide; 3-{[5-(azetidin-l-ylcarbonyl)ρyridin-3-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-[(l-methylethyl)oxy]-N-(l-methyl-lH- pyrazol-3-yl)benzamide;
3-{ [6-(azetidin- 1 -ylcarbonyl)pyridin-3-yl]oxy}-5-[(l -methylethyl)oxy]-N- 1 ,3-thiazol-2- ylbenzamide;
3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3-yl] oxy } -5-[( 1 -methylethyl)oxy] -N-( 1 -methyl- 1 H- pyrazol-3-yl)benzamide; 3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3 -yl]oxy }-5-{[(lS)-l -methyl-2-
(methyloxy)ethyl]oxy } -N- 1 ,3-thiazol-2-ylbenzamide;
3-{ [6-(azetidin-l -ylcarbonyl)pyridin-3-yl]oxy }-5-{ [(I S)- 1 -methyl-2-
(methyloxy)ethyl]oxy}-N-(3-methyl-l,2,4-thiadiazol-5-yl)benzamide; 3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-[(l-methylethyl)oxy]-N-(3-methyl-l,2,4- thiadiazol-5-yl)benzamide;
3-{[5-(azetidin-l-ylsulfonyl)ρyridin-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{[(lS)-l-methyl-2- (methyloxy)ethyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-l,3-thiazol-2-yl]oxy}-5-[(l-methylethyl)oxy]-N-(l-methyl-
1 H-pyrazol-3 -yl)benzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)pyridin-2-yl]oxy } -5 - { [( 1 S)-2-hydroxy- 1 -methylethyl] oxy } -
N-(l-methyl-lH-pyrazol-3-yl)benzamide; 3 - { [3 -chloro-5 -(morpholin-4-ylcarbonyl)pyridin-2-yl]oxy } -5 - { [( 1 S)-2-hydroxy- 1 - methylethyl] oxy } -N-( 1 -methyl- 1 H-pyrazol-3 -yl)benzamide ; and
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[2-fluoro-l-
(fluoromethyl)ethyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide; and/or
3-{[5-(azetidin-l-ylcarbonyl)-l,3-thiazol-2-yl]oxy}-5-{[(lS)-l-methyl-2- (methyloxy)ethyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl]oxy } -5-{[(lS)-l -methylpropyl] oxy } -N-
( 1 -methyl- 1 H-pyrazol-3 -yl)benzamide ;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{[(lS)-l-methylpropyl]oxy}-N-(l- methyl- 1 H-pyrazol-3 -yl)benzamide; 3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-{[(lS)-l-methylpropyl]oxy}-N-(l- methyl- 1 H-pyrazol-3 -yl)benzamide;
3-{[2-(azetidin-l-ylcarbonyl)pyrimidin-5-yl]oxy}-5-[(l-methylethyl)oxy]-N-(l-methyl-
1 H-pyrazol-3 -yl)benzamide ;
3- { [2-(azetidin- 1 -ylcarbonyl)pyrimidin-5-yl]oxy }-5- { [(I S)- 1 -methyl-2- (methyloxy)ethyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide;
3- { [6-(azetidin- 1 -ylcarbonyl)pyridin-3-yl]oxy }-5- { [2-fluoro- 1 -(fluoromethyl)ethyl]oxy } -
N-(I -methyl- 1 H-pyrazol-3 -yl)benzamide; 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{[2-fluoro-l-(fluoromethyl)ethyl]oxy}-
N-(I -methyl- 1 H-pyrazol-3 -yl)benzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)pyrazin-2-yl] oxy } -5 - [( 1 -methylethyl)oxy] -N-( 1 -methyl- 1 H- pyrazol-3-yl)benzamide; 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-({(lS)-2-
[(difluoromethyl)oxy] - 1 -methylethyl } oxy)-N-( 1 -methyl- 1 H-pyrazol-3 -yl)benzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)pyridin-2-yl] oxy} -5-({(lS)-2- [(difluoromethyl)oxy] - 1 - methylethyl } oxy)-N-( 1 -methyl- 1 H-pyrazol-3 -yl)benzamide;
3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3 -yl]oxy } -5 -({ ( 1 S)-2-[(difluoromethyl)oxy]- 1 - methylethyl } oxy)-N-( 1 -methyl- 1 H-pyrazol-3 -yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-({(lS)-2-[(difluoromethyl)oxy]-l- methylethyl } oxy)-N-( 1 -methyl- 1 H-pyrazol-3 -yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(lR,2R)-2-hydroxy-l- methylpropyl]oxy } -N-( 1 -methyl- 1 H-pyrazol-3 -yl)benzamide; 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(lS,2S)-2-hydroxy-l- methylpropyl] oxy } -N-( 1 -methyl- 1 H- pyrazol-3 -yl)benzamide ;
3 - { [5-(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl] oxy } -N-( 1 -ethyl- 1 H-pyrazol-3 -yl)-5 -
{ [( 1 S)- 1 -methyl-2-(methyloxy)ethyl]oxy }benzamide;
3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3 -yl]oxy } -N-( 1 -ethyl- 1 H-pyrazol-3-yl)-5 - {[(ΙS)-I- methyl-2-(methyloxy)ethyl]oxy}benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-N-(l-ethyl-lH-pyrazol-3-yl)-5-{[(lS)-l- methyl-2-(methyloxy)ethyl]oxy}benzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl] oxy } -5 - { [( 1 S)-2-hydroxy- 1 - methylethyl]oxy}-N-[l-(l-methylethyl)-lH-pyrazol-3-yl]benzamide; 3-{[5-(azetidin-l-ylcarbonyl)pyridin-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-lH-pyrazol-3-ylbenzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)-5-
[(3S)-tetrahydroruran-3-yloxy]benzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)-5-[(3S)- tetrahydroruran-3-yloxy]benzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl] oxy } -N-( 1 -methyl- 1 H-pyrazol-3-yl)-5 - [(3 S)- tetrahydrofuran-3-yloxy]benzamide; 3- { [4-(azetidin- 1 -ylcarbonyl)- 1 ,3-thiazol-2-yl]oxy } -5-{ [(I S)- 1 -methyl-2-
(methyloxy)ethyl] oxy } -N-( 1 -methyl- 1 H-pyrazol-3-yl)benzamide ;
3-{[4-(azetidin-l-ylcarbonyl)-l,3-thiazol-2-yl]oxy}-5-[(l-methylethyl)oxy]-N-(l-methyl- lH-pyrazol-3-yl)benzamide; and 3 - { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy } -5 -{[(1S)-1 -(hydroxymethyl)propyl] oxy } -
N-(I -methyl- 1 H-pyrazol-3 -yl)benzamide; and/or
3-{[2-(azetidin-l-ylcarbonyl)pyrimidin-5-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N- 1 ,3-thiazol-2-ylbenzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-(cyclopentyloxy)-N-(l-methyl-lH- pyrazol-3-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-(cyclopentyloxy)-N-(l-methyl-
1 H-pyrazol- 3 -yl)benzamide ;
3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3 -yl]oxy } -5 -(cyclopentyloxy)-N-( 1 -methyl- 1 H- pyrazol-3-yl)benzamide; 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{[(lR,2R)-2-hydroxy-l- methylproρyl]oxy } -N-(I -methyl- 1 H-pyrazol-3-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{[(lS,2S)-2-hydroxy-l- methylpropyl]oxy } -N-(I -methyl- 1 H-pyrazol-3-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-l,3-thiazol-2-yl]oxy}-5-{[(lS)-l-methyl-2- (methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy } -5 -{[( IS)-I -methyl-2-
(methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide; 3-{[5-(azetidin-l-ylcarbonyl)pyridin-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide;
3 - { [4-(azetidin- 1 -ylcarbonyl)- 1 ,3 -thiazol-2-yl]oxy } -5 - { [( 1 S)- 1 -methyl-2-
(methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)-4-methyl- 1 ,3 -thiazol-2-yl]oxy } -5- { [( 1 S)- 1 -methyl-2- (methyloxy)ethyl] oxy } -N-(5-methylpyrazin-2-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-4-methyl-l,3-thiazol-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy } -N-(I -methyl- 1 H-pyrazol-3-yl)benzamide; 3 - { [5 -(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl]oxy } -5-[( 1 -methylethyl)oxy] -N-(5 - methylpyrazin-2-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-[(l-methylethyl)oxy]-N-(5- methylpyrazin-2-yl)benzamide ; 3-{[5-(azetidin-l-ylcarbonyl)pyridin-2-yl]oxy}-5-[(l-methylethyl)oxy]-N-(5- methylpyrazin-2-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-4-methyl-l,3-thiazol-2-yl]oxy}-5-[(l-niethylethyl)oxy]-N-
(5 -methylpyrazin-2-yl)benzamide ;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-{[(lS)-l-methyl-2- (methyloxy)ethyl]oxy} -N-(5-methylpyrazin-2-yl)benzamide;
3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3 -yl] oxy } -5 - [( 1 -methylethyl)oxy] -N-(5- methylpyrazin-2-yl)benzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)-3-chloropyridin-2-yl] oxy } -5- { [( 1 S)- 1 -methyl-2-
(methyloxy)efhyl]oxy}-N-lH-pyrazol-3-ylbenzamide; 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl] oxy } -N- 1 ,3 -thiazol-2-ylbenzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-pyridin-2-ylbenzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-N-(l-ethyl-lH-pyrazol-3-yl)-5- [(l-methylethyl)oxy]benzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy } -N-( 1 -ethyl- 1 H-pyrazol-3 -yl)-5- [( 1 - methylethyl)oxy]benzamide;
3- { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy } -N-[I -(I -methylethyl)- 1 H-pyrazol-3-yl]-5-
{[(lS)-l-methyl-2-(methyloxy)ethyl]oxy}benzamide; 3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-N-(l-ethyl-lH-pyrazol-3-yl)-5-[(l- methylethyl)oxy]benzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-pyridin-2-ylbenzamide;
3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3 -yl] oxy } -N-isoxazol-3 -yl-5 - { [( 1 S)- 1 -methyl-2- (methyloxy)ethyl]oxy}benzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl]oxy } -5-[( 1 -methylethyl)oxy] -N-(3 - methyl-l,2,4-thiadiazol-5-yl)benzamide; 3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-[(l-methylethyl)oxy]-N-lH-pyrazol-3- ylbenzamide;
3-{[6-(azetidin-l-ylcarbonyl)ρyridin-3-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-lH-pyrazol-3-ylbenzamide; 3 - { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy } -5-[( 1 -methylethyl)oxy] -N- 1 H-pyrazol-3 - ylbenzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-lH-pyrazol-3-ylbenzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl]oxy } -5 - [( 1 -methylethyl)oxy] -N-IH- pyrazol-3-ylbenzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-N-(l,5-dimethyl-lH-pyrazol-3- yl)-5-{[(lS)-l -methyl-2-(methyloxy)ethyl] oxy }benzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl] oxy } -N-(1 ,5-dimethyl- 1 H-pyrazol-3 - yl)-5- { [2-fluoro- 1 -(fluoromethyl)ethyl]oxy }benzamide; 3-{[6-(azetidin-l-ylcarbonyl)pyridazin-3-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-N-(4-methyl-l,3-thiazol-2-yl)-5-[(3S)- tetrahydrofuran-3 -yloxy]benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-N-(5-methyl-l,3-thiazol-2-yl)-5-[(3S)- tetrahydrofuran-3-yloxy]benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-N-(l-ethyl-lH-pyrazol-3-yl)-5-[(3S)- tetrahydrofuran-3-yloxy]benzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy } -N- [ 1 -( 1 -methylethyl)- 1 H-pyrazol-3 -yl] -5 -
[(3S)-tetrahydrofuran-3-yloxy]benzamide; S-ltS-Cazetidin-l-ylcarbony^pyrazin^-y^oxyj-N-lH-pyrazol-S-yl-S-tCSS)- tetrahydrofuran-3-yloxy]benzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy } -N-( 1 ,5-dimethyl- 1 H-pyrazol-3 -yl)-5 -[(3 S)- tetrahydrofuran-3-yloxy]benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-N-(4-methyl-l,3-thiazol-2-yl)-5- [(3S)-tetrahydrofuran-3-yloxy]benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-N-(5-methyl-l,3-thiazol-2-yl)-5-
[(3S)-tetrahydrofuran-3-yloxy]benzamide; 3 - { [5 -(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl] oxy } -N-(5-methylpyrazin-2-yl)-5 -
[(3S)-tetrahydrofuran-3-yloxy]benzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl] oxy } -N-( 1 -ethyl- 1 H-pyrazol-3 -yl)-5 -
[(3S)-tetrahydrofuran-3-yloxy]benzamide; 3 - { [5 -(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl] oxy } -N- [ 1 -( 1 -methylethyl)- 1 H- pyrazol-3-yl]-5-[(3S)-tetrahydrofuran-3-yloxy]benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-N-lH-pyrazol-3-yl-5-[(3S)- tetrahydrofuran-3-yloxy]benzamide;
3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3 -yl]oxy } -N-(5-methylpyrazin-2-yl)-5 - [(3 S)- tetrahydrofuran-3-yloxy]benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-N-(5-methylpyrazin-2-yl)-5-[(3S)- tetrahydrofuran-3-yloxy]benzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-N-(5-fluoropyridin-2-yl)-5-[(3S)- tetrahydrofuran-3 -yloxy]benzamide; 3-{ [3-chloro-5-(pyrrolidin- 1 -ylcarbonyl)pyridin-2-yl]oxy}-5-[(l -methylethyl)oxy]-N-(5- methylpyrazin-2-yl)benzamide;
3-{[3-chloro-5-(pyrrolidin-l-ylcarbonyl)pyridin-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl] oxy } -N-(5 -methylpyrazin-2-yl)benzamide;
3 - { [3 -chloro-5-(pyrrolidin- 1 -ylcarbonyl)pyridin-2-yl]oxy } -N-( 1 -methyl- 1 H-pyrazol-3 -yl)- 5-[(3S)-tetrahydrofuran-3-yloxy]benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)-5-
(tetrahydro-2H-pyran-4-yloxy)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)-5-
(tetrahydro-2H-pyran-4-yloxy)benzamide; 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-N-(5-methylpyrazin-2-yl)-5-(tetrahydro-
2H-pyran-4-yloxy)benzamide;
3-{ [5-(azetidin- 1 -ylcarbonyl)-3-chloropyridin-2-yl]oxy}-N-(5-methylpyrazin-2-yl)-5-
(tetrahydro-2H-pyran-4-yloxy)benzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)-5- (tetrahydro-2H-pyran-4-yloxy)benzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-N-(5-rnethylpyrazin-2-yl)-5-(tetrahydro-
2H-pyran-4-yloxy)benzamide; 3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-N-(l-methyl-lH-pyrazol-3-yl)-5-[(3R)- tetrahydrofuran-3-yloxy]benzamide; and/or
3-{ [6-(azetidin- 1 -ylcarbonyl)pyridin-3-yl]oxy}-N-isoxazol-3-yl-5-[(3 S)-tetrahydrofuran-3- yloxy]benzamide; 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-N-pyrazin-2-yl-5-[(3S)-tetrahydrofuran-3- yloxy]benzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)pyrazin-2-yl] oxy } -N-(5 -fluoropyridin-2-yl)-5- [(3 S)- tetrahydrofuran-3-yloxy]benzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy } -5 - { [( 1 S)- 1 -methyl-2- (methyloxy)ethyl]oxy}-N-pyridin-2-ylbenzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy } -N-isoxazol-3 -yl-5 - { [( 1 S)- 1 -methyl-2-
(methyloxy)ethyl] oxy }benzamide ;
3 - { [5 -(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy } -5- { [( 1 S)- 1 -methyl-2-
(methyloxy)ethyl]oxy}-N-(4-methyl-l,3-thiazol-2-yl)benzamide; 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl] oxy } -N-(5 -methyl- 1 ,3 -thiazol-2-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazm-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-pyrazin-2-ylbenzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-[(l-methylethyl)oxy]-N-pyrazin-2- ylbenzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-pyrazin-2-ylbenzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl]oxy } -5-[( 1 -methylethyl)oxy] -N- pyrazin-2-ylbenzamide ; 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-pyrazin-2-ylbenzamide;
3- { [3 -chloro-5 -(moφholin-4-ylcarbonyl)pyridin-2-yl]oxy } -N-( 1 -methyl- 1 H-pyrazol-3 -yl)-
5-[(3S)-tetrahydrofuran-3-yloxy]benzamide;
3-{[5-(7-azabicyclo[2.2.1]hept-7-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-N-(l-methyl-lH- pyrazol-3-yl)-5-[(3S)-tetrahydrofuran-3-yloxy]benzamide; and
3-{[5-(azetidin-l-ylcarbonyl)-4-methyl-l,3-thiazol-2-yl]oxy}-N-(l-methyl-lH-pyrazol-3- yl)-5-[(3S)-tetrahydrofiαran-3-yloxy]benzamide; and/or 3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-{[(lS)-2-hydroxy-l-methylethyl]oxy}-
N-(5-methylpyrazin-2-yl)benzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-{[(lS)-2-hydroxy-l-methylethyl]oxy}-
N-(3 -methyl- 1 ,2,4-thiadiazol-5-yl)benzamide; 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(lS)-2-hydroxy-l- methylethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[(lS)-2-hydroxy-l- methylethyljoxy }-N-(3 -methyl- 1 ,2,4-thiadiazol-5-yl)benzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl]oxy } -5 - { [( 1 S)-2-hydroxy- 1 - methylethyl]oxy}-N-pyrazin-2-ylbenzamide;
3 - { [5 -(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl]oxy } -5- { [( 1 S)-2-hydroxy- 1 - methylethyl]oxy}-N-pyridin-2-ylbenzamide;
3-{ [5-(azetidin- 1 -ylcarbonyl)pyridin-2-yl]oxy } -5- { [(I S)-2-hydroxy- 1 -methylethyl]oxy }-
N-(3-methyl-l,2,4-thiadiazol-5-yl)benzamide; N-(I -methyl- 1 H-pyrazol-3 -yl)-3 - { [6-(pyrrolidin- 1 -ylcarbonyl)pyridin-3-yl] oxy } -5-[(3 S)- tetrahydrofuran-3-yloxy]benzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-N-pyrazin-2-yl-5-[(3S)-tetrahydrofuran-3- yloxy]benzamide;
3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-N-(5-methyl-lH-pyrazol-3-yl)-5-[(3S)- tetrahydrofuran-3-yloxy]benzarnide;
3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3 -yl]oxy } -N- 1 H-pyrazol-3 -yl-5 - [(3 S)- tetrahydrofiiran-3 -yloxy]benzamide;
3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[2-fluoro-l-
(fluoromethyl)ethyl] oxy } -N-(5 -methylpyrazin-2-yl)benzamide; 3-{[5-(azetidin-l-ylcarbonyl)-3-chloropyridin-2-yl]oxy}-5-{[2-fluoro-l-
(fluoromethyl)ethyl]oxy}-N-lH-pyrazol-3-ylbenzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)-3 -chloropyridin-2-yl] oxy } -5 - { [2-fluoro- 1 -
(fluoromethyl)ethyl]oxy}-N-(5-methyl-lH-pyrazol-3-yl)benzamide;
3 - { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl] oxy } -5 - { [2-fluoro- 1 -(fluoromethyl)ethyl]oxy } - N-lH-pyrazol-3-ylbenzamide;
3- { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy } -5- { [2-fluoro- 1 -(fluoromethyl)ethyl]oxy } -
N-(5-methylpyrazin-2-yl)benzamide; 3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-{[(lS)-2-hydroxy-l-methylethyl]oxy}-
N-(5-methylpyrazin-2-yl)benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazin-2-yl]oxy}-5-({(lS)-2-[(difluoromethyl)oxy]-l- methylethyl}oxy)-N-lH-pyrazol-3-ylbenzamide; 3-{[5-(azetidin-l-ylsulfonyl)-4-methyl-l,3-thiazol-2-yl]oxy}-5-{[(lS)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide;
3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3 -yl]oxy } -N-[I -(difluoromethyl)- 1 H-pyrazol-3 -yl] -5-
[(3S)-tetrahydrofuran-3-yloxy]benzamide;
3-{[5-(azetidin-l-ylcarbonyl)pyrazm-2-yl]oxy}-N-[l-(difluoromethyl)-lH-pyrazol-3-yl]-5- {[(1S)-1 -methyl-2-(methyloxy)ethyl] oxy }benzamide;
3 - { [6-(azetidin- 1 -ylcarbonyl)pyridin-3-yl] oxy } -N-[ 1 -(difluoromethyl)- 1 H-pyrazol-3 -yl]-5 -
{[(lS)-l-methyl-2-(methyloxy)ethyl]oxy}benzamide; and/or
3- { [5-(azetidin- 1 -ylcarbonyl)pyrazin-2-yl]oxy } -5- { [(1-K)- 1 -methyl-2-
(methyloxy)ethyl] oxy } -N-(5 -methylpyrazin-2-yl)benzamide; 3-{[6-(azetidin-l-ylcarbonyl)pyridin-3-yl]oxy}-5-{[(ljR)-l-methyl-2-
(methyloxy)ethyl]oxy}-N-(5-methylpyrazin-2-yl)benzamide; or a salt thereof.
9. A pharmaceutical composition comprising a compound according to any one of Claims 1 to 8, or a pharmaceutically-acceptable salt thereof, together with a pharmaceutically acceptable diluent or carrier.
10. A compound according to any one of Claims 1 to 8 or a pharmaceutically- acceptable salt thereof for use as a medicament.
11. A compound according to Claim 10, wherein the medicament is a medicament for treatment of a disease mediated through GLK, in particular type 2 diabetes.
12. The use of a compound according to any one of Claims 1 to 8, or a pharmaceutically-acceptable salt thereof, in the preparation of a medicament for treatment of a disease mediated through GLK.
13. The use of a compound according to any one of Claims 1 to 8, or a pharmaceutically-acceptable salt thereof, in the preparation of a medicament for treatment of type 2 diabetes.
14. A method of treating GLK mediated diseases by administering an effective amount of a compound of Formula (I) or (IA) as claimed in any one of Claims 1 to 8 or a pharmaceutically-acceptable salt thereof, to a mammal in need of such treatment.
15. The method of Claim 14 wherein the GLK mediated disease is type 2 diabetes.
16. A process for the preparation of a compound of Formula (I) or (IA) as claimed in any one of Claims 1 to 8, which comprises a process a) to e) (wherein the variables are as defined for compounds of Formula (I) in Claim 1 unless otherwise stated):
(a) reaction of an acid of Formula (III) or activated derivative thereof with a compound of Formula (IV), wherein R1 is as hereinbefore defined or a protected version thereof;
Figure imgf000264_0001
or (b) reaction of a compound of Formula (V) with a compound of Formula (VI),
Figure imgf000264_0002
(V) (VI) wherein X1 is a leaving group and X2 is a hydroxyl group or X1 is a hydroxyl group and X2 is a leaving group, and wherein R1 is as hereinbefore defined or a protected version thereof; process (b) could also be accomplished using the intermediate ester Formula (VII), wherein P1 is a protecting group as hereinafter described, followed by ester hydrolysis and amide formation by procedures described elsewhere and well known to those skilled in the art;
Figure imgf000265_0001
(V) (VII) or
(c) reaction of a compound of Formula (VIII) with a compound of Formula (IX)
Figure imgf000265_0002
(VIII) (IX) wherein X3 is a leaving group or an organometallic reagent and X4 is a hydroxyl group or X3 is a hydroxyl group and X4 is a leaving group or an organometallic reagent, and wherein R1 is as hereinbefore defined or a protected version thereof; process (c) could also be accomplished using the intermediate ester Formula (X), followed by ester hydrolysis and amide formation by procedures described elsewhere and well known to those skilled in the art;
Figure imgf000265_0003
(VIII) (X) (d) reaction of a compound of Formula (XI) with a compound of Formula (XII),
Figure imgf000266_0001
(XI) (XII); wherein X5 is a leaving group; and wherein R1 is as hereinbefore defined or a protected version thereof; or e) reaction of a compound of formula (XIII)
Figure imgf000266_0002
(XIII) wherein R2a is a precursor to R2, such as a carboxylic acid, ester or anhydride (for R2 = -CONR4R5) or the sulfonic acid equivalents (for R2 is -SO2NR4R5); with an amine of formula -NR4R5; and thereafter, if necessary: i) converting a compound of Formula (I) into another compound of Formula (I); ii) removing any protecting groups; and/or iii) forming a salt thereof.
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NO20076658A NO20076658L (en) 2005-07-09 2007-12-28 Heteroberl benzamide derivatives for use as GLK activators in the treatment of diabetes
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US12/203,324 US7842694B2 (en) 2005-07-09 2008-09-03 Heteroaryl benzamide derivatives for use as GLK activators in the treatment of diabetes
US12/256,254 US7977328B2 (en) 2005-07-09 2008-10-22 Heteroaryl benzamide derivatives for use as GLK activators in the treatment of diabetes
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