WO2006136540A2

WO2006136540A2 - Human skin regenerating ointment comprising annelid extracts

Info

Publication number: WO2006136540A2
Application number: PCT/EP2006/063320
Authority: WO
Inventors: Víctor MELINAO CANALES
Original assignee: Incubadora De Negocios Gesta Mayor, S.A.; Barlocci, Anna
Priority date: 2005-06-20
Filing date: 2006-06-19
Publication date: 2006-12-28
Also published as: WO2006136540A3

Abstract

The invention relates to a pharmaceutical or cosmetic composition comprising annelid extracts; to a method for its preparation, and to its use for treating dermatological affections and in surgical or cosmetic treatments. Annelid extract are obtained from earth annelids and/or water annelids. The composition is of natural origin, free of additives; it is useful for treating a series of affections at dermis and epidermis levels, such as: skin affections (injuries, new or old scars, hot water or fire burns, herpes); surgical treatments (scores, tissue repair and healing, skin fortification, pain mitigation, etc.) or cosmetic ones (stimulate pigmentation, acting on antioxidant agents, protection against sun radiation, and others).

Description

Human skin regenerating ointment

FIELD OF INVENTION

The present invention relates to the preparation and use of an easy-to-apply human skin regenerating ointment, the composition of which is of natural origin, free o additives and obtained from earth and/or water annelids. The ointment itself is useful for treating a series of affections at dermis and epidermis levels, such as: skin affections (injuries, new or old scars, hot water or fire burns, herpes); surgical treatments (scores, tissue repair and healing, skin fortification, pain mitigation, etc.) or cosmetologic ones (stimulate pigmentation, acting on anti-oxidant agents, protection against sun radiation, and others).

BACKGROUND OF THE INVENTION Various products, both of a natural and chemical synthetic origin, are available in the market that are useful to treat, individually, different skin affections, derived from surgical actions, accidents, and others, or to be used for cosmetic purposes, forcing the medical staff, and mainly, patients or those interested to acquire and apply a series of products for the same affection.

There exist independent studies on regenerating-capacity compositions obtained in vitro (US 20040063205). Compositions based on natural Trichuris suis sources with antibacterial activity, yet not used as a dermatological ointment, have also been described (US 20040029802). Also known are some emulsions of natural origin containing hemoglobin or hemoglobin/myoglobin in the form of cream or lotion for treating poorly oxygenated skin (US 20040022839).

In addition, there are individual studies on the properties of worms wherein they separately use their components to account for specific affections, not for all of them (US 20040063205, US 20040029802, US 20040022839, US 20060047126, US 2005255522, US 20040247603, US 20040147606, US 20040081955). Instead, the present skin regenerating ointment acts at various levels (healing, analgesic, etc) and in a new area, such as skin regeneration.

There is Chilean patent CL 38615 that discloses a natural original cream for use in cosmetology, which is obtained from snail bave. In addition, Chilean patent application CL 1739-03 discloses a pharmaceutical or cosmetic gel composition comprising snail bave, carbopol, water and preservatives, to treat skin aging, spots, scars, burns, and other affections.

There also are commercially available natural origin products for treating skin affections, and some surgical or cosmetologic treatments, such as snail oil (snail-based, Reference: Skin Care Masterpiece), emu oil (base on emu, Reference: EMU Chile S.A), hazelnuts or Rosa Mosqueta oil and/or cream hazelnut and Rosa Mosqueta, Reference: Pharmos Natur (r), Primal Nature Products (r)). Their main characteristics are based on their composition and the properties discovered therein. Thus, emu oil has a high content of linololeic oil, oleic and palmitic oil helping to stimulate new cells, lower or attenuate spots on the skin. Snail cream helps hydration, stopping skin, as Rosa Mosqueta and hazelnut do. However, these products, except for emu oil, generally operate at dermis level.

As for synthetic products, there exist a variety of specific products on the market for each one of the phases of skin affections, surgical or cosmetologic treatments.

Heretofore, no natural origin composition obtained from earth/water annelids has been made for use to treat skin affections.

As for the farming of annelids, they have extensively been cultured since antiquity via a technique referred to as Worm Farming for the production of: humus, vermoplast, worm meal as fish feed for salmon farming or treating sewage (Lombriastur M2660557, USPTO 20050090405 USPTO 20040065610, Lombricultura.com, Lombricor.com). However, the species produced to this end is Eisenia foetida only, better known as red wiggler.

DESCRIPTION OF THE INVENTION

The present invention relates to the preparation of an easy-to-apply human skin regenerating ointment, the composition of which is 100% natural, since it is an extract of natural organisms, specifically earth and/or water annelids.

According to one aspect, the invention relates to a pharmaceutical or cosmetic composition comprising annelid extracts.

According to another aspect, the invention relates to a method of preparing a pharmaceutical or cosmetologic composition which comprises the collection of annelids and attainment of the extract from annelids which are the raw material of said composition.

According to a further aspect, the invention relates to the use of the above pharmaceutical composition for the manufacture of a medicament for treating dermatological affections and for surgical or cosmetologic treatments.

The present invention uses a very simple extraction technique, wherein a natural composition is obtained, which is comprised of a number of components characteristic of its source, and this composition is useful to prepare an oil, cream, ointment or powder, useful for treating dermatological affections, surgical or cosmetologic treatments, at dermis and epidermis level.

The pharmacological ands/or cosmetic action of this ointment is the result of the different elements comprising this extract.

Embodiments of the human skin regenerating ointment are characterized, depending on its composition, in that the base raw materials are earth and/or water annelids, mainly of the Prosoporos and Opistoporos Family, such as: Lumbiculida, of the Lumbriculidae, Branchiobdelidae, Acanthobdelodae, Alluroididae, Sygeonodrilidae, Monologastridae, Lumbricidae, Dorydrilidae families; being also able to be from the Monolidastήde, haplotaxidae, Enchytraeidae, Tubificidae, Naididae, Phreodrilidae, Opostocystidae, Alluroididae, Syngenodrilodae, Biwadrolidae, Criodrilidae, Sparganophilidae, Ailoscolecidae, Hormogastridae, Lumbricidae, Lutodrilidae, Kynotodae, Mocrochaetodae, Glossoscolecidae, Almidae, Onerodrilidae, Megascolecidae, Eudrilidae, Aelosomatodae , Aelosomatodae, Naidodae, Oposthocystidae, Tubibificidae,Phreodrolodae, Enchytraeidae families.

Raw material collection of earth and/or water annelids for processing the Human Skin Regenerating Ointment is done on fields or land rich in non- contaminated animal or vegetal wastes, by selecting, depending on the year season, a quantity from 150 to 800 annelids whose weight and length varies based on the collecting season.

Once the annelids have been collected, they are washed in plastic containers with abundant deep well water and then weighed. In the winter, 200 annelids ca weigh, all together, between 150 and 400 grams, which, later, are put into an aluminum, steel or iron pot exposed to low heat, the temperature of which varies between 40° a 70° degrees Celsius, for a period between 1 and 10 minutes.

50 to 70 ml or ointment is obtained in liquid, creamy or solid state, depending on the state of maturity of the annelids and the working temperature.

The skin regenerator ointment is a compound of natural origin obtained through an easily executable, fast and low-cost method of extraction, providing great advantages to the pharmaceutical and cosmetic industries.

The annelid-extract obtained by the present invention serves to prepare useful compositions for treating dermatological affections, such as: hot water or fire burns, healing of injuries (new or old), scores; at surgical level (tissue regeneration after operations); reinforcement of muscle tissues; at cosmetic level, skin strengthening, pigmentation improvement, and protection against radiation and oxidizing agents

In the prior art, the treatment applied to a first or second degree burn from fire, hot water or any element in contact with a hand, is the use of medicines containing: (benzocaine (infection), analgesics (pain), anti inflammatories (inflammation), vitamin E (skin regeneration), (valine and isoleucine (tissue repair and production of (L-Glutamine), which means, at least, three different medicines applied on a single burn.

The advantage of the present invention is that the annelid extract used to prepare a skin regenerating ointment has various simultaneous active principles, since the skin regenerating ointment prevents infection, and acts both as an anti-inflammatory and a skin regenerator.

Using the skin regenerator obtained from annelids only requires the additional use of analgesics during the first day of treatment, which presents a great advantage with respect to the already described compositions for treating bums, in addition to the easy-to-produce and low-cost advantages. Other relevant advantages of the preset invention are: low cost, fast attainment of the raw material and easy preparation of the extract. The attainment of annelids to prepare the extract presents great advantages with respect to other inventions based on the extraction of natural products, such as emu oil. The attainment of annelids has advantages, not only the easy acquisition of these organisms from earth and water, but also because the farming of annelids is known for other purposes, and has been widely developed, living place to worm farming, annelids show a high reproduction rate, they reproduce at an average rate on 1 to 20 individuals per year and, in adequate condition, they may breed at a rate of 1 ,000 to 4,000 individuals per square meter of earth..

One comparative advantage is that the extract of the present invention shows a composition having a mono- and poly-saturated oil content much higher than emu oil and other natural extracts used for skin care treatment.

Another comparative advantage with respect to emu oil, is the high content of Omega 3 oil in the annelid extract as compared to the emu oil used in skin care treatments.

Another advantage is the effect the extract exerts not only at dermis level, but also at the epidermis one.

EXAMPLE 1

Production of a human skin regenerating extract.

The base raw material for this example is earth annelids of the prosopore, opistopore family, Lumbricullina, Haplotaxina, Lumbricina and Megascolecina type.

1. Earth annelids were collected on land abundant with non contaminated animal or vegetal wastes, located in the San Patricio sector, Cautfn Province, Araucanfa Region, Chile.

2. 200 annelids specimens were obtained, which were washed in plastic containers with abundant untreated water (non-chlorinated, from a deep well).

3. The annelids were washed and weighed, and 190 g. were obtained, which were subject to a temperature from 45° to 65° C, in a 1 liter stainless steel container, for a 10-minute period. 4. A final quantity of 70ml was obtained. This extract may be used directly as a skin regenerating ointment or for preparing a composition containing pharmaceutically acceptable additives, known in the state of the art in compositions for dermatological purposes.

Composition of the extract obtained

Table I: Annelid extract composition

Analysis on a 200-annelid sample, conducted by Laboratorio Diamolab Ltda.

Table II: Annelid amino-acid extract composition

The Skin Regenerating ointment contains 90% of essential human amino- acids.

Table III: Composition of the saturated fatty acids in the annelid extract

Table IV: Mono-saturated fatty acid composition in annelid extract

Table V: Poly-saturated fatty acid composition in annelid extract

Analysis conducted by ANALAB CHILE S.A, as report on results Nr. 161968, dated 01-06-2006.

The concentration of fatty and saturate acids in the annelid extract obtained is low, but it is high in mono-saturated and poly-saturated oils.

EXAMPLE 2

Use of annelid extract to treat skin burns.

In a farm, in the borough of Vilcύn, a worker, aged 37, together with two other workers, was painting a shed cover with hot tar, when a zinc roof tile suddenly collapsed and he and the hot tar bucket fell from a 10-meter height. The left side of his face and part of his scalp got covered with a film of hot tar, which was party removed by his workmates, and the rest of the material was removed at the Vilcύn and Temuco hospitals.

After the accident, he remained in hospital for 3 months due to 2^nd and 3^rd degree burns, after which period he was released until the infection and pain had been controlled. No skin change or face skin regeneration treatment was carried out.

He continued an out-patient treatment with only disinfectants and analgesics. After 2 months of out-patient treatment, the treatment applied at Temuco's Hernan Henrfquez hospital was discontinued. The patient had pain and great sensibility on the affected areas and the burns had healed.

Based on this condition, the ointment of the invention, annelid extract, was applied on a daily basis.

The annelid extract was obtained on a daily basis by heating two annelids in a 15 ml capacity stainless steel container.

Heating took place in a furnace or in a thermal-regulated bath.

The extract obtained was directly applied on the skin, being manually spread on face and head, on the damaged part, twice a day, over a three-month period.

The results, after a three-month long treatment were as follows:

• Face skin recovery on the affected zone.

• Recovery of part of the scalp lost as a result of the burn.

• Recovery of skin pigmentation and strength.

The annelid extract of the present invention did not regenerate part of the lost ear due to the effect of the hot tar, and no hair was regenerated on the rear part of the head, where the greatest damage took place and the burn was deeper (3^rd degree).

The additional advantages of applying the annelid extract, over the three- month long treatment were that the patient did not suffer from infections on the injure and pains disappeared.

EXAMPLE 3

Use of annelid extract to treat injuries.

The annelid extract obtained was applied on field workers' minor injuries. The kind of injuries treated is from damaged caused by barbed wire on the workers' hands, back and head, which take place when these workers have to release animals entangled in fences.

The extract applied is obtained by heating two annelids in a 15 ml capacity stainless steel container inside a furnace at 40-60 ⁰C.

The treatment on injuries at dermis level consisted of the application, twice a day (morning and afternoon), of the extract for three days, period in which injuries properly healed. These injuries were treated with annelid extract as the only treatment, without applying other skin regenerating compositions or analgesics.

Claims

1. A pharmaceutical or cosmetic composition comprising annelid extracts.

2. The pharmaceutical or cosmetic composition in accordance with claim 1 , wherein the annelid extract comes from earth annelids and/or water annelids.

3. The pharmaceutical or cosmetic composition in accordance with claim 1 , wherein it comprises an annelid extract that is additive-free.

4. The pharmaceutical or cosmetic composition, in accordance with claim 1 , wherein the annelid extract comprises about 70% of proteins, about 7% of lipids, about 20% of carbon hydrates, and about 0.7% of ashes.

5. The pharmaceutical or cosmetic composition, in accordance with claim 4, wherein the lipid fraction comprises about 30% of saturated fatty acids, about 40% of fatty mono-saturated acids, and about 30% of fatty poly- saturated acids.

6. A Method of preparing a pharmaceutical or cosmetologic composition wherein it comprises the collection of annelids and attainment of the extract from annelids which are the raw material of said composition.

7. The method of preparing a pharmaceutical or cosmetologic composition, in accordance with claim 6, wherein the collection of annelids comprises earth annelids and/or water annelids.

8. The method of preparing a pharmaceutical or cosmetologic composition, in accordance with claim 6, wherein the extract is attained through the steps of:

• washing the annelids with chlorine-free water, and • heating the collected annelids.

9. The method of preparing a pharmaceutical or cosmetologic composition, in accordance with claim 8, wherein heating is applied at a 45° to 65° C temperature range.

10. The method of preparing a pharmaceutical or cosmetologic composition, in accordance with claim 8, wherein heating is applied for a 1 to 10- minute period.

1 1. Use of the pharmaceutical composition of claim 1 for the manufacture of a medicament for treating dermatological affections and for surgical or cosmetologic treatments.

12. Use in accordance with claim 1 1 , for the manufacture of a medicament for treating dermis and epidermis affections.