WO2006067864A1

WO2006067864A1 - Proanthocyanidin-containing composition

Info

Publication number: WO2006067864A1
Application number: PCT/JP2004/019788
Authority: WO
Inventors: Kinya Takagaki; Takeshi Mitsui
Original assignee: Toyo Shinyaku Co., Ltd.
Priority date: 2004-12-24
Filing date: 2004-12-24
Publication date: 2006-06-29

Abstract

A composition comprising a proanthocyanidin and a catechin having a trihydroxyphenyl. This composition realizes striking enhancement of the various physiological activities of proanthocyanidin.

Description

Proanthocyanidin-containing composition

Technical field

The present invention relates to a composition for enhancing the bioactive action of proanthocyanidins.

Akita

Background art

Proanthocyanidins are condensed or polymerized tannins (hereinafter referred to as polycondensation) present in various plants, and are a group of compounds obtained by polycondensation using flavan 1-ol or flavan 1,3-diol as a constituent unit. is there. These are given their names because they produce anthocyanidins such as cyanidin, delphinidin, and pelargonidin by acid treatment. Among proanthocyanidins, a polycondensation polymer having a degree of polymerization of 2 to 4 is called oligomeric proanthocyanidin (hereinafter referred to as OPC).

Proanthocyanidins are a kind of polyphenols and have a strong antioxidant action (Japanese Patent Laid-Open No. Sho 61-16982), and further improve blood lipids; antihypertensive effects; Effect of suppressing blood sugar level rise; Effect of restoring blood vessel elasticity

(Anti-vascular effect); It is known to have an effect of increasing the utilization efficiency of vitamin C; and an effect of improving blood fluidity.

In recent years, in order to effectively utilize various bioactive effects of such proanthocyanidins, combinations with other components have been studied, and the bioactive actions of proanthocyanidins are enhanced and synergistic with other diseases and diseases. Have been found (Japanese Patent Laid-Open No. 2003-146 8 98, Japanese Patent Laid-Open No. 2003-9596 5, JP-A-2002-275076, JP-A-2503107, JP-A-5-112441, JP-A-11-80009 and JP-A-2002-238497). However, there is a need for means capable of enhancing not only such actions but also various physiologically active actions of proanthocyanidins.

Disclosure of the invention

An object of the present invention is to provide a novel composition that can further enhance various physiologically active actions of proanthocyanidins.

The composition of the present invention is characterized by containing proanthocyanidins and catechins having a 3,4,5-trihydroxyphenyl group.

The composition of the present invention contains proanthocyanidins and catechins having a 3,4,5-trihydroxyphenyl group.

In a preferred embodiment, the 3, 4, 5_trihydroxyphenino group is a galloyl group.

Oral or transdermal administration of a composition containing the above-mentioned proanthocyanidins and trihydroxenyl group-containing power textiles to improve the blood lipids and lipid metabolism of proanthocyanidins It can remarkably enhance physiological activities such as blood glucose level rise inhibitory effect, antihypertensive effect, blood flow improving effect, and skin beautifying effect. These effects are excellent effects that cannot be obtained by single administration of catechins having a proanthocyanidin and a trihydroxyphenyl group, respectively. The composition of the present invention can be effectively used for foods, pharmaceuticals, quasi drugs, cosmetics and the like. Brief Description of Drawings

Figure 1 shows a rat with a pine bark extract and a category with trihydroxyphenyl groups. Feeds containing quinine, pine bark extract and catechin not containing trihydroxyphenyl group, and feed containing only pine bark extract 2 It is a graph which shows transition of the blood pressure increase rate. BEST MODE FOR CARRYING OUT THE INVENTION

Hereinafter, the composition of the present invention will be described. The configurations described below are not intended to limit the present invention, and it will be apparent to those skilled in the art that various modifications can be made within the spirit of the present invention.

The composition of the present invention contains proanthocyanidins and catechins having a 3, 4, 5_trihydroxyphenyl group, and optionally contains other components. Hereinafter, each component will be described.

(Proanthocyanidins)

As the proanthocyanidins used in the present invention, those containing a large amount of a condensation polymer having a low degree of polymerization are suitable. As the condensation polymer having a low degree of polymerization, a condensation polymer having a degree of polymerization of 2 to 30 (a 2 to 30 mer) is preferable, and a condensation polymer having a degree of polymerization of 2 to 10 (a 2 to 10 mer). ) Is more preferred, and a condensation polymer having a degree of polymerization of 2 to 4 (2 to 4 mer: OPC) is more preferred. OPC is a particularly powerful antioxidant. Proanthocyanidins are concentrated in plant leaves, bark, fruit peels or seeds. Proanthocyanidins, especially OPCs, are specifically the bark of plants such as pine, straw, and yamatake; grapes, blueberries, strawberries, avocados, false acacia, berries or seeds of barley; wheat; soybeans; black It is contained in soybeans, cacao, red beans, tochi nut shells, peanut skins, ichiyo leaves, etc. Also, West African cola nuts, Peruvian Latania roots. Japanese green tea is known to contain OPC. OPC is a substance that cannot be produced in the human body. In particular, when an extract containing proanthocyanidins with a high OPC content or proanthocyanidins with a high OPc content is used, proanthocyanidins with a high degree of polymerization (those with a low OPC content) are used. In contrast, excellent lipid metabolism improvement effect, antihypertensive effect, blood glucose level rise suppression effect, etc. are obtained. As the proanthocyanidins used in the composition of the present invention, materials such as the bark of the above-mentioned plant, pulverized fruits or seeds, or extracts thereof can be used. Among these, it is preferable to use an extract derived from a plant because it contains abundant proanthocyanidins, and it is particularly preferable to use an extract derived from pine bark. Among the plants containing proanthocyanidins, pine bark is rich in OPC, so it is preferably used as a raw material for proanthocyanidins. The extract is preferably one from which impurities have been removed. Hereinafter, an example of using pine bark rich in OPC as a raw material plant will be described as a method for preparing an extract containing puffer anthocyanidin as a main component.

Pinus martima, larch, black pine, waka pine, Japanese pine, pine pine, Korean pine, high pine, Ryuyuki pine, Utsushima pine, Daiyu pine, white pine, Quebec in Canada An extract of a bark of a plant belonging to the order of pine is preferably used. Above all, the bark extract of French coastal pine (Pinus Martima) is preferred.

French coastal pine is a marine pine that grows on the Atlantic coast of southern France. This French coastal pine bark contains proanthocyanidins, organic acids, and other physiologically active ingredients. Proanthocyanidins, which are the main ingredients, have strong antioxidative effects to remove active oxygen. It is known that there is. The pine bark extract is obtained by extracting the above pine bark with water or an organic solvent. When water is used, it is preferable to use warm water or hot water. To improve the extraction efficiency, salt such as sodium chloride is added to these waters. And are preferred. As the organic solvent used for extraction, organic solvents that are acceptable for the production of foods or pharmaceuticals are used. For example, methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, acetone, hexane , Cyclohexane, propylene glycol, water-containing ethanol, water-containing propylene glycol, methyl ethyl ketone, glycerin, methyl acetate, ethyl acetate, jetyl ether, dichloromethane, edible fats and oils, 1, 1, 1, 2-tetrafluoro And 1,1,1,2-trichloroethene. These water and organic solvents may be used alone or in combination. In particular, water, hot water, ethanol, water-containing ethanol, and water-containing propylene glycol are preferable, and water, hot water, ethanol, and water-containing ethanol are more preferable from the viewpoint of safety when used in foods and pharmaceuticals.

The method for extracting proanthocyanidins from pine bark is not particularly limited. For example, by extracting 1 to 50 parts by weight of hot water of 50 to 120 ° C., preferably 70 to 100 ° C. with respect to 1 part by weight of pine bark dry, A pine bark extract having a high bioactivity and high water solubility can be obtained. A warm extraction method, a supercritical fluid extraction method, or the like may be used.

Alternatively, supercritical fluid extraction may be performed by an entrainer addition method. In this method, for example, ethanol, propanol, n-hexane, acetone, toluene, other aliphatic lower alcohols, aliphatic hydrocarbons, aromatic hydrocarbons, or ketones are added to a supercritical fluid. Add about 2 O WZV% and perform supercritical fluid extraction with the resulting extraction fluid to dramatically increase the solubility of the target extract, such as OPC and catechins (described later), in the extraction solvent. Alternatively, it is a method for enhancing the selectivity of separation, and a method for efficiently obtaining a pine bark extract.

For extraction from pine bark, you can combine several extraction methods. It is possible to obtain pine bark extracts with various compositions by combining multiple extraction methods. It becomes ability.

The pine bark extract obtained by the above extraction may be purified for the purpose of increasing the content of proanthocyanidins. For purification, a solvent such as ethyl acetate is usually used, but from the viewpoint of safety as a food or pharmaceutical, a method that does not use a solvent, such as ultrafiltration or Diaion HP-2O, Sephadex It is preferable to purify by a column method or a batch method using an adsorbent carrier such as LH 20 or chitin.

In the present invention, the pine bark extract containing proanthocyanidin as a main component is specifically prepared by the following method, but this is an example and is not limited to this method.

1 kg of French coastal pine bark is placed in 3 L of saturated sodium chloride solution and extracted at 100 ° C for 30 minutes to obtain an extract (extraction process). Thereafter, the extract is filtered, and the resulting insoluble matter is washed with 50 O mL of a saturated solution of sodium chloride to obtain a washing solution (washing step). The extract and washing solution are combined to obtain a crude extract of pine bark.

Next, 25 mL of ethyl acetate is added to the crude extract and the phases are separated to recover the ethyl acetate layer 5 times. Combine the recovered ethyl acetate solution and add directly to 200 g of anhydrous sodium sulfate for dehydration. Then, the ethyl acetate solution is filtered, and the filtrate is concentrated under reduced pressure until the original volume is reduced to one-fifth. The concentrated ethyl acetate solution is poured into 2 L of chloroform and the precipitate obtained by stirring is collected by filtration. Then, after the precipitate is dissolved in 10 mL of ethyl acetate, the washing step is repeated twice, in which the precipitate is added again to 1 L of chloroform and precipitated. According to this method, for example, about 5 g of pine bark extract containing 20% by weight or more of OPC and 5% by weight or more of strong techins can be obtained.

Extracts derived from raw material plants such as the above pine husks contain 40% by weight or more of pro It is preferable to contain anthocyanidin. Further, the extract derived from the raw material plant preferably contains 20% by weight or more of OPC, more preferably 30% by weight or more. As described above, a pine bark extract is preferably used as a raw material containing proanthocyanidins in a high proportion.

Since OPC is an antioxidant, it has the effect of reducing the risk rate of adult diseases such as cancer, heart disease, and cerebral thrombosis, and has the effect of improving the allergy in nature such as arthritis, atopic dermatitis, and hay fever. In addition, it suppresses bacterial growth in the oral cavity and reduces plaque (dental glands); restores the elasticity of blood vessels; prevents lipoproteins in the blood from being damaged by active oxygen; It also has the effect of preventing cholesterol from adhering to the inner wall of blood vessels and adhering cholesterol; the effect of regenerating vitamin E decomposed by active oxygen; and the effect of enhancing vitamin E.

(Catechin having 3, 4, 5-trihydroxyphenyl group)

The composition of the present invention contains catechins having a 3,4,5-trihydroxyphenyl group (hereinafter sometimes referred to as THP catechins). Catechin is a generic name for polyhydroxyflavan 1-ol, (+) — force techin (referred to as catechin in a narrow sense), gallocatechin, afuzerekin, (+) 3-galloyl derivative of The 3-galloyl derivative of yopi gallocatechin is common.

Examples of THP catechins include (+)-gallocatechin, (1) one-epigalocatechin, force-techin gallate, epicatechin gallate, gallocatechin gallate, and epigallocatechin gallate. Of these THP catechins, catechins in which the trihydroxyphenyl group is a galloyl group are preferred. Such catechins having a galloyl group include force techin gallate, epicatechin gallate, gallocatechin gallate, and epigallocatechin gallate. Etc.

THP catechins are contained in plants such as camellias (for example, tea leaves such as green tea, black tea, and ulong tea) and mao. These plants or their meals or extracts may be used as THP catechins. Preferably, it is a camellia plant, or a pulverized product thereof or an extract thereof. For example, green tea leaves contain about 10-15% catechins, of which about 80% or more are THP catechins and about 50% or more are TH P catechins. Since it is a catechin having one galloyl group, it is preferable.

THP catechins, together with proanthocyanidins, can remarkably enhance various bioactive effects of proanthocyanidins. Thus, the inventors found for the first time that THP catechins are materials that efficiently enhance the multiple bioactive effects of proanthocyanidins. In particular, catechins having a galloyl group not only have high antioxidant activity, but also have excellent effects such as an inhibitory effect on blood glucose level increase, antihypertensive effect, anti-lipaemia, and the like, so proanthocyanidins It is possible to remarkably enhance various physiologically active actions possessed by.

THP catechins are other anti-carcinogenic effects, arteriosclerosis-preventing effects, fat-metabolism-suppressing effects, blood pressure rise-inhibiting effects, platelet aggregation-inhibiting effects, anti-allergic effects, anti-viral effects, anti-bacterial effects, caries-preventing effects, bad breath It is known to have an inhibitory action, an intestinal microbiota normalizing action, an active oxygen and free radical scavenging action, and an antioxidant action.

(Other ingredients)

The composition of the present invention contains the above-mentioned proanthocyanidins and THP catechins, and may contain various components as necessary. For example, normal food and medicine Ingredients that can be added as excipients (excipients, extenders, binders, thickeners, emulsifiers, colorants, fragrances, nutritional ingredients, food additives, etc.); and usually as external preparations for skin of cosmetics, quasi drugs Ingredients used (whitening agent, moisturizer, antioxidant, UV absorber, UV scattering agent, antiseptic, fragrance, gelling agent, polymer, oil component, surfactant, thickener, alcohol, Powder components, colorants, aqueous components, various skin nutrients, etc.).

Nutritional components include, for example, ascorbic acid, tocopherol, riboflavin, vitamins such as 3 -carotene, folic acid, and piotin; minerals such as calcium, magnesium, selenium, iron; included in taurine, garlic, etc. Sulfur compounds; Flavanoids such as hesperidin and quercetin; Flavonoids; Dietary fibers such as indigestible dextrin, alginic acid, chitin, chitosan and guagam; Proteins such as soy protein and collagen; Peptides; Amino acids; Milk Animal fats such as fat, lard, beef tallow and fish oil; vegetable oils such as soybean oil and rapeseed oil; fruits such as orange, lemon, grapefruit and strawberry and their juices; royal jelly and propolis. Examples of food-added calories include honey, reduced maltose, lactose, sugar alcohol, liquid sugar, and seasonings.

(Proanthocyanidin-containing composition)

As described above, the composition of the present invention contains proanthocyanidins and THP strength textiles and, if necessary, other ingredients. As described above, proanthocyanidins and THP catechins may be any of isolated compounds, food materials containing them, and extracts of the food materials. For example, a plant extract containing THP strength is added to a plant extract containing proanthocyanidins. Specifically, it contains proanthocyanidins such as pine bark and cinnamon bark, and most of the catechins contained are trihydroxide. A plant extract containing THP catechins is added to a plant extract that is a catechin that does not have a siphenyl group. If the plant extract contains both proanthocyanidins and THP catechins, add necessary ingredients (for example, pine bark extract containing a large amount of proanthocyanidins) depending on the purpose. The amount ratio of each component in the composition can be adjusted as appropriate.

The content ratio of proanthocyanidins and THP catechins in the composition of the present invention is arbitrary. Preferably, with respect to 100 parts by weight of proanthocyanidins, the above-mentioned THP strength techins are 1 part by weight to 5000 parts by weight, more preferably 3 parts by weight to 2000 parts by weight, still more preferably 10 parts by weight to 1000 parts by weight, Most preferably, it can be contained in a proportion of 50 to 500 parts by weight.

There is no restriction | limiting in particular in content of proanthocyanidin in the composition of this invention. Preferably, it is preferably contained so that the daily intake per adult is 1 to 1000 mg, more preferably 2 to 500 mg as proanthocyanidins. In the case of transdermal administration, since it is a topical administration, it is preferable to have a predetermined concentration. Specifically, proanthocyanidins are preferably contained in the composition in an amount of preferably 0.0001% to 20% by weight. More preferably, it may be contained in a proportion of 0.0001% to 10% by weight, more preferably 0.001% to 10% by weight, and most preferably 0.01% to 10% by weight.

There is no restriction | limiting in particular in content of THP catechin in the composition of this invention. It is preferable that the daily intake for adults is 10 mg to: I 0000 mg, more preferably 20 mg to 500 Omg. In the case of transdermal administration, as with proanthocyanidins, since it is a topical administration, it is preferable to have a predetermined concentration.Specifically, THP catechins are preferably 0. 00001 wt% to 20 wt%, more preferably 0.001 wt% to 20 wt%, more preferably 0.001 wt% to 20 wt%, most preferably 0.01 wt% to 20 wt% May be contained. The composition of the present invention can be prepared in various forms depending on the purpose, for example, as a food, a pharmaceutical, a quasi-drug, or a cosmetic.

Among the above-mentioned uses, when the composition of the present invention is orally ingested (administered) as food, pharmaceuticals, quasi drugs, etc., there is no particular limitation on the form. For example, capsules such as hard capsules and soft capsules, tablets, pills, powder (powder), granules, tea bags, bowl-like viscous liquids, liquids, pastes, etc. are used in a form commonly used by those skilled in the art. . Depending on the shape or preference, these may be taken as they are, or may be taken by dissolving in water, hot water, milk, etc., or may be taken after leaching the ingredients.

Even when the composition of the present invention is applied to the skin as a quasi-drug, cosmetic, etc., these forms are not particularly limited, such as ointments, creams, emulsions, lotions, packs, poultices, bath preparations, etc. Any form can be used as long as it is conventionally used for an external preparation for skin. Example

Hereinafter, the present invention will be described with reference to examples, but it goes without saying that the present invention is not limited to the examples.

In this example, pine husk extract containing 40% by weight of proanthocyanidins as a material containing proanthocyanidins (containing 20% by weight of OPC in the material) (trade name: Flavandinol, Toyo Shinyaku Co., Ltd.) ) Was used. The THP catechins, catechin 8 0 weight ^_0/0 containing green tea-derived extract (trade name, containing these forces catechins having Garoi Le group 6 0 wt% or more: Porifuenon 7 0 S, Tokyo Food Techno Co., Ltd.) and an extract derived from green tea containing 93% by weight of Epigalocatechin Gallate (trade names: Theavigo, Roche “Vitamin” Japan Co., Ltd.) were used. Here, since the above-mentioned “polyphenone 70 S” contains 48% by weight or more of THP-powered tekins, Referred to as the "tea catechin 48", "Teabiko" is, the THP force catechins from that it contains 93 by weight ^_0/0 or more, Les as "tea catechin 93", to Ukoto. Catechin (Sigma Aldrich Japan) was used as a catechin having no trihydroxyphenyl group.

(Example 1)

By mixing the following materials were prepared purified diet: corn starch 29.49 by weight ^_0/0, milk casein 20 wt%, Alf alpha meal 10 weight ^_0/0, - potato starch 10 weight ^_0/0, cellulose powder 10 weight ^_0/0, Shiyukuro scan 10 weight ^_0/0, soybean 6 wt ^_0/0, mineral mixture (Α Ι Ν- 76) 3. 5 by weight ^_0/0, vitamin mixture (Α Ι Ν- 76) 1 wt ^_0/0, and Asukorubin acid 0.01 wt%. These were mixed with refined feed so that the pine peel extract was 1.0% by weight and tea catechin 48 was 0.5% by weight. And).

(Example 2)

A solid feed was prepared in the same manner as in Example 1 except that tea catechin 93 was used instead of tea catechin 48 (referred to as feed 2). (Comparative Example 1)

A solid feed was prepared in the same manner as in Example 1 except that catechin was used instead of tea catechin 48 (referred to as feed 3).

(Comparative Example 2)

A solid feed was prepared in the same manner as in Example 1 except that tea catechin 48 was not mixed (referred to as feed 4). (Comparative Example 3)

A solid feed was prepared in the same manner as in Example 1 except that the pine bark extract was not mixed (referred to as feed 5).

(Example 3: Blood lipid improving effect and lipid cost improving effect)

Three-week-old male guinea pigs (Japan SLC Co., Ltd.) were given a commercial solid feed (RC4, Oriental Yeast Co., Ltd.) for one week to acclimate. The guinea pigs were divided into groups of 7 by a total randomization method. Each group of guinea pigs was allowed free intake of the above feeds 1-5 for 28 days. Furthermore, as a control group, a group that freely ingested purified feed was established. After the intake period, the total cholesterol level, LDL cholesterol level, HDL cholesterol level, and triglyceride level in the blood of each group of guinea pigs were measured. The results are shown in Table 1. The measurement method is as follows.

Total cholesterol (mgZd L) was measured by enzymatic method using “Serotech” TCHO-L (manufactured by Serotech Co., Ltd.).

LDL cholesterol (mgZd L) was measured by an enzyme method using Cholestest LDL (Daiichi Chemical Co., Ltd.).

HDL cholesterol (mg / d L) was measured by enzymatic method using Choleest HDL (Daiichi Chemical Co., Ltd.).

Triglyceride (mgZd L) was measured by an enzymatic method using “Cellotech” TG-L (manufactured by Seguchi Tech Co., Ltd.). table 1

Average soil standard deviation

From the results in Table 1, the group that ingested the feed (feeds 1 and 2) containing the pine bark extract of the example and the coconut catechins showed that the pine bark extract of the comparative example and the trihydroxyphenyl group (ΤΗΡ group) Compared with the group ingesting feed containing catechins without feed (feed 3), or feed containing only one of pine bark extract and THP catechins (feeds 4 and 5), It can also be seen that the triglyceride content is low. Furthermore, it can be seen that among cholesterol, the blood concentration of HDL cholesterol, which is said to be good, is high. These facts indicate that the composition containing proanthocyanidins and THP catechins has excellent blood lipid improvement effects and lipid metabolism improvement effects that cannot be obtained when taken alone. Show.

(Example 4)

Mix with basic feed (MF feed, Oriental Yeast Co., Ltd.) so that pine bark extract is 0.5% by weight and tea catechin 48 is 0.5% by weight. Prepared (referred to as feed 6). (Comparative Example 4)

A solid feed was prepared in the same manner as in Example 4 except that catechin was used instead of tea catechin 48 (referred to as feed 7).

(Comparative Example 5)

A solid feed was prepared in the same manner as in Example 4 except that tea catechin 48 was not mixed (referred to as feed 8).

(Example 5: blood glucose level increase suppressing effect)

One group of 5 4-week-old SD rats (Japan SLC Co., Ltd.) were allowed to freely take the above feeds 6 to 8 for 2 weeks each. In addition, a group that freely ingested basic feed was established as a control group. After the intake period, I fasted for 12 hours. Thereafter, a glucose tolerance test was performed by forcibly administering a 20 wt% glucose solution to each group of rats so that the glucose was 25 O mg / 100 g body weight. The blood glucose level immediately before the load test and the blood glucose level 60 minutes after the load test were measured to evaluate the effect of suppressing the increase in blood glucose level. The results are shown in Table 2. Table 2

Blood glucose level (mg dL)

Before the gel course load test After the course test Before and after the load test

AB BA feed 6 Example 4 148 182 34 Feed 7 Comparative example 4 152 201 49 Feed 8 Comparative example 5 153 198 45 Basic feed Control example 154 217 63 From the results shown in Table 2, the group that ingested the feed (feed 6) containing the pine bark extract of the example and the THP catechins had the pine bark extract of the comparative example and the trihydroxyphenyl group (THP. Group). It can be seen that the increase in blood glucose level is suppressed as compared with the group ingesting the feed containing catechin not contained (Feed 7) or the feed containing only the pine bark extract (Feed 8).

From the results in Table 1 and Table 2 above, the composition containing pine bark extract and THP catechins has an effect of improving blood lipids and suppressing an increase in blood glucose level. Is considered to be obtained.

(Example 6: Antihypertensive effect)

Four-week-old male SHR rats (SHR and other disease model joint study group) were acclimated with a basic diet and water for one week. The rats were divided into 7 groups, so that the average fit of each group was almost uniform. The rats in each group were allowed to freely take the above diets 6-8 for 28 days. In addition, a group with free intake of basic feed was established as a control group. During the intake period, all groups received free drinking water containing 1% by weight of NaC1.

Using the non-invasive automatic sphygmomanometer (BP-98A: soft mouth), the systolic blood pressure of the rats in each group was measured on days 7, 14, 21, and 28, starting on the day of feeding. Measured. The rate of increase in systolic blood pressure after the lapse of each feeding day relative to the systolic blood pressure on the feeding start day was calculated from the following equations. Figure 1 shows the change in the rate of increase in systolic blood pressure. Increase rate of systolic blood pressure ₍ % ₎ = (systolic blood pressure after feeding) _{χΊ 00}

(Systolic blood pressure of the feeding start cocoon) From the results of Fig. 1, the group ingested the diet (feed 6) containing the pine bark extract of the example and the coconut catechins, the pine bark extract of the comparative example and the chicken Droxif It can be seen that the increase in blood pressure was suppressed compared to the group that ingested the feed containing catechins that do not have an enyl group (THP group) (Feed 7) or the feed containing only pine bark extract (Feed 8). . This indicates that the yarn and composition containing pine bark extract and THP catechins is effective as a prophylactic / therapeutic agent for hypertension.

(Example 7)

Pine bark extract, tea catechin 93, and excipients are mixed in the proportions (weight%) shown in Table 3 to produce tablets (about 200 mg per tablet). Yes.

(Comparative Examples 6 and 7)

In the same manner as in Example 7, the ingredients listed in Table 3 were mixed to produce tablets (about 200 mg per tablet). Table 3

Unit is% by weight

* 1 Contains 93% by weight or more of THP catechins

* 2 ······· Does not contain catechins (Example 8: Blood flow improvement effect)

Twenty-four healthy men and women aged between 22 and 63 years old (12 men and 12 women) were the subjects, and they were randomly divided into three groups, except for equal gender. For 8 subjects in each group, blood flow was first measured before food intake. The subjects then took the above foods 1-3 (approximately 20 Omg per tablet) at a rate of 2 tablets per day with 10 OmL of water. This ingestion was carried out for 28 days, and after the ingestion period, blood flow was measured again. The blood flow is measured on the subcutaneous part of the index finger of the right hand.

(Laser blood flow imaging device PIM II; Swedish company). In addition, I avoided eating and drinking for 3 hours before the measurement and asked me to rest. The results are shown in Table 4. The values in Table 4 are average standard error. The larger the value, the greater the blood flow. Table 4

Average soil standard deviation

From the results in Table 4, the group that ingested the food (food 1) containing the pine husk extract of the example and the potato catechins had the pine bark extract of the comparative example and the trihydroxyphenyl group (ΤΗΡ). It can be seen that the blood flow is greatly increased compared to the group that ingested the food containing catechin (food 2) or the food containing only pine bark extract (food 3). This indicates that the composition containing pine bark extract and repulsive textiles has an effect of improving blood flow in peripheral tissues. ,

(Example 9)

Pine lotion extract, tea catechin 48, alcohol, glycerin, and 1,3-butylene glycol were dissolved in purified water in the proportions (% by weight) shown in Table 5 to prepare a lotion. This was named lotion 1.

(Comparative Examples 8 and 9)

In the same manner as in Example 9, the ingredients listed in Table 5 were mixed and dissolved in purified water to prepare a skin lotion. These were designated as lotions 2 and 3. Table 5

Unit is% by weight

The balance is purified water

* 1 "'THP catechins more than 48% by weight

(Example 10: Skin beautifying effect)

Randomly divided into 8 groups of 18 women who were worried about rough skin (stains, wrinkles, and kusumi). The above-mentioned lotion 1 to 3 (2 mL) was applied once a day before going to bed at a place where the skin of the subjects in each group was concerned. This application was performed for 28 days. At the end of the application period, we asked the self-assessment of whether there was an improvement effect on stains, stains, and kusumi, and counted the number of people who clearly had an improvement effect on each item. The result Table 6 shows, Table 6

From the results shown in Table 6, the group to which the skin lotion (lotion 1) containing the pine bark extract of the example and the THP catechins was applied was either one of the pine bark extract of the comparative example and the THP strength techin. It can be seen that skin wrinkles, spots, and kusumi are improved compared to the group to which the skin lotion containing only the skin lotion (skin lotions 2 and 3) is applied. This indicates that the pine peel extract and trihydroxif 2 It shows that a composition containing a catechin having a ru group has a beautifying effect. Industrial applicability

The composition of the present invention can be administered orally (taken) or transdermally (applied) to improve the lipid metabolism of proanthocyanidins, improve blood lipids, suppress blood glucose elevation, and antihypertensive. Physiologically active effects such as blood flow improvement effect and skin beautifying effect can be remarkably enhanced. These effects are excellent effects that cannot be obtained when proanthocyanidins and THP strength techins are administered alone. The composition of the present invention can be used for foods, pharmaceuticals, quasi drugs, cosmetics and the like.

Claims

The scope of the claims

1. A composition containing proanthocyanidins and catechins having a 3, 4, 5-trihydroxyphenyl group.

2. The composition according to claim 1, wherein the 3,4,5-trihydroxyphenyl group is a galloyl group.