WO2006040142A3 - Verfahren zum nachweisen und/oder zum bestimmen der konzentration mindestens eines liganden - Google Patents

Verfahren zum nachweisen und/oder zum bestimmen der konzentration mindestens eines liganden Download PDF

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Publication number
WO2006040142A3
WO2006040142A3 PCT/EP2005/010976 EP2005010976W WO2006040142A3 WO 2006040142 A3 WO2006040142 A3 WO 2006040142A3 EP 2005010976 W EP2005010976 W EP 2005010976W WO 2006040142 A3 WO2006040142 A3 WO 2006040142A3
Authority
WO
WIPO (PCT)
Prior art keywords
ligand
radiation
measurement signal
receptor
solution
Prior art date
Application number
PCT/EP2005/010976
Other languages
English (en)
French (fr)
Other versions
WO2006040142A2 (de
Inventor
Mirko Lehmann
Ingo Freund
Holger Klapproth
Sonja Mohry
Original Assignee
Micronas Gmbh
Micronas Holding Gmbh
Mirko Lehmann
Ingo Freund
Holger Klapproth
Sonja Mohry
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Micronas Gmbh, Micronas Holding Gmbh, Mirko Lehmann, Ingo Freund, Holger Klapproth, Sonja Mohry filed Critical Micronas Gmbh
Priority to US11/665,169 priority Critical patent/US20080138829A1/en
Priority to EP05803501A priority patent/EP1807700A2/de
Publication of WO2006040142A2 publication Critical patent/WO2006040142A2/de
Publication of WO2006040142A3 publication Critical patent/WO2006040142A3/de

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

Bei einem Verfahren zum Nachweisen und/oder zum Bestimmen der Konzentration eines in einer zu analysierenden Lösung (1) enthaltenen Liganden (2) wird auf einem Halbleiterchip (4) an einer Teststelle (3) ein Rezeptor (5) immobilisiert, der mit dem Liganden (2) eine spezifische Bindung eingehen kann. Zum Binden des Liganden (2) an den Rezeptor (5) wird die Lösung (1) auf die Teststelle (3) aufgebracht. In Abhängigkeit von der Bindung des Liganden (2) an den Rezeptor (5) wird eine Lumineszenzstrahlung (14) oder eine Farbveränderung erzeugt. Mit Hilfe eines in den Halbleiterchip (4) integrierten Strahlungsempfängers (6) wird ein Strahlungsmesssignal erfasst, während die Lösung (1) und/oder eine den Luminophor (17) nicht enthaltende Hilfsflüssigkeit mit dem Strahlungsempfänger (6) in Kontakt steht. Während das Erzeugen der Lumineszenzstrahlung verhindert wird und der Strahlungsempfänger (6) mit der Hilfsflüssigkeit und/oder einer Ersatzflüssigkeit in Kontakt steht, wird ein Dunkelmesssignal für den Strahlungsempfänger (6) gemessen. Das Strahlungsmesssignal wird mit dem Dunkelmesssignal kompensiert.
PCT/EP2005/010976 2004-10-13 2005-10-12 Verfahren zum nachweisen und/oder zum bestimmen der konzentration mindestens eines liganden WO2006040142A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US11/665,169 US20080138829A1 (en) 2004-10-13 2005-10-12 Method For Detecting and/or Determining the Concentration of at Least One Ligand
EP05803501A EP1807700A2 (de) 2004-10-13 2005-10-12 Verfahren zum nachweisen und/oder zum bestimmen der konzentration mindestens eines liganden

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102004050032.0 2004-10-13
DE102004050032A DE102004050032A1 (de) 2004-10-13 2004-10-13 Verfahren zum Nachweisen und/oder zum Bestimmen der Konzentration mindestens eines Liganden

Publications (2)

Publication Number Publication Date
WO2006040142A2 WO2006040142A2 (de) 2006-04-20
WO2006040142A3 true WO2006040142A3 (de) 2006-08-10

Family

ID=36087895

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/010976 WO2006040142A2 (de) 2004-10-13 2005-10-12 Verfahren zum nachweisen und/oder zum bestimmen der konzentration mindestens eines liganden

Country Status (4)

Country Link
US (1) US20080138829A1 (de)
EP (1) EP1807700A2 (de)
DE (1) DE102004050032A1 (de)
WO (1) WO2006040142A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006060748A2 (en) 2004-12-03 2006-06-08 California Institute Of Technology Microfluidic sieve valves
EP2020601B1 (de) 2007-08-03 2010-11-17 Micronas GmbH Kit und Vorrichtung zum Erzeugen einer Chemolumineszenzstrahlung
WO2009132667A1 (de) * 2008-04-30 2009-11-05 Micronas Gmbh Verfahren zum nachweisen und/oder bestimmen der konzentration eines liganden

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5827748A (en) * 1997-01-24 1998-10-27 The United States Of America As Represented By The Secretary Of The Navy Chemical sensor using two-dimensional lens array
WO2002073158A2 (en) * 2001-03-09 2002-09-19 Apollo Biotechnology, Inc. Conjugate probes and optical detection of analytes

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8420053D0 (en) * 1984-08-07 1984-09-12 Secr Social Service Brit Enhanced luminescent/luminometric assay
US5846708A (en) * 1991-11-19 1998-12-08 Massachusetts Institiute Of Technology Optical and electrical methods and apparatus for molecule detection
DE19736641A1 (de) * 1997-08-22 1999-03-11 Michael G Dr Weller Verfahren und Vorrichtung zur parallelen Messung von mehreren Analyten in komplexen Mischungen
DE19925402C2 (de) * 1999-06-02 2001-12-20 Molecular Machines & Ind Gmbh Screening von Target-Ligand-Wechselwirkungen
DE10109779A1 (de) * 2001-03-01 2002-09-19 Infineon Technologies Ag Vorrichtung und Verfahren zum Erfassen von makromolekularen Biopolymeren mittels mindestens einer Einheit zum Immobilisieren von makromolekularen Biopolymeren
DE10161529A1 (de) * 2001-12-14 2003-07-03 Infineon Technologies Ag Biosensor zum Erfassen von makromolekularen Biopolymeren, Sensoranordnung mit einem Biosensor, Verfahren zur Herstellung des Biosensors und Verfahren zum Erfassen von Nukleinsäuremolekülen mittels mindestens einer Einheit zum Immobilisieren von Nukleinsäuren
EP1406090A1 (de) * 2002-10-02 2004-04-07 Infineon Technologies AG Optoelektronischer Sensor insbesondere zur Detektion von makromolekularen Biopolymeren
DE10251757B4 (de) * 2002-11-05 2006-03-09 Micronas Holding Gmbh Vorrichtung zur Bestimmung der Konzentration von in einer zu untersuchenden Probe enthaltenen Liganden
US7113285B2 (en) * 2003-12-09 2006-09-26 Beckman Coulter, Inc. Multimode reader

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5827748A (en) * 1997-01-24 1998-10-27 The United States Of America As Represented By The Secretary Of The Navy Chemical sensor using two-dimensional lens array
WO2002073158A2 (en) * 2001-03-09 2002-09-19 Apollo Biotechnology, Inc. Conjugate probes and optical detection of analytes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KEGELMEYER L M ET AL: "A GROUNDTRUTH APPROACH TO ACCURATE QUANTITATION OF FLUORESCENCE MICROARRAYS", PROCEEDINGS OF THE SPIE, SPIE, BELLINGHAM, VA, US, vol. 4266, 2001, pages 35 - 45, XP001156764, ISSN: 0277-786X *
MENDIS D ET AL: "Cell biotinylation provides a sensitive and effective detection technique for cellular adhesion assays: comparison with existing methods", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 253, no. 1-2, 1 July 2001 (2001-07-01), pages 57 - 68, XP004241360, ISSN: 0022-1759 *

Also Published As

Publication number Publication date
WO2006040142A2 (de) 2006-04-20
US20080138829A1 (en) 2008-06-12
DE102004050032A1 (de) 2006-04-27
EP1807700A2 (de) 2007-07-18

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