WO2006036735A3 - Procede de detection et de quantification de multiples composants subcellulaires - Google Patents

Procede de detection et de quantification de multiples composants subcellulaires Download PDF

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Publication number
WO2006036735A3
WO2006036735A3 PCT/US2005/033964 US2005033964W WO2006036735A3 WO 2006036735 A3 WO2006036735 A3 WO 2006036735A3 US 2005033964 W US2005033964 W US 2005033964W WO 2006036735 A3 WO2006036735 A3 WO 2006036735A3
Authority
WO
WIPO (PCT)
Prior art keywords
detecting
subcellular components
quantitating multiple
quantitating
multiple subcellular
Prior art date
Application number
PCT/US2005/033964
Other languages
English (en)
Other versions
WO2006036735A2 (fr
Inventor
Michael Kilpatrick
Triantafyllos Tafas
Original Assignee
Ikonisys Inc
Michael Kilpatrick
Triantafyllos Tafas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ikonisys Inc, Michael Kilpatrick, Triantafyllos Tafas filed Critical Ikonisys Inc
Priority to JP2007533625A priority Critical patent/JP2008518587A/ja
Priority to AU2005289765A priority patent/AU2005289765A1/en
Priority to EP05798226A priority patent/EP1791979A4/fr
Priority to CA002580961A priority patent/CA2580961A1/fr
Publication of WO2006036735A2 publication Critical patent/WO2006036735A2/fr
Publication of WO2006036735A3 publication Critical patent/WO2006036735A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/689—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to pregnancy or the gonads
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Gynecology & Obstetrics (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

La présente invention se rapporte à un procédé permettant de détecter et de quantifier de multiples signaux fluorescents uniques issus d'un échantillon cellulaire. Le procédé selon l'invention combine les techniques d'immunohistochimie et d'hybridation in situ à marquage fluorescent. Le procédé selon l'invention est utile pour identifier des composants subcellulaires spécifiques de cellules telles que des chromosomes et des protéines.
PCT/US2005/033964 2004-09-22 2005-09-22 Procede de detection et de quantification de multiples composants subcellulaires WO2006036735A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2007533625A JP2008518587A (ja) 2004-09-22 2005-09-22 多細胞下成分の検出及び定量法
AU2005289765A AU2005289765A1 (en) 2004-09-22 2005-09-22 Method for detecting and quantitating multiple subcellular components
EP05798226A EP1791979A4 (fr) 2004-09-22 2005-09-22 Procede de detection et de quantification de multiples composants subcellulaires
CA002580961A CA2580961A1 (fr) 2004-09-22 2005-09-22 Procede de detection et de quantification de multiples composants subcellulaires

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61206704P 2004-09-22 2004-09-22
US60/612,067 2004-09-22

Publications (2)

Publication Number Publication Date
WO2006036735A2 WO2006036735A2 (fr) 2006-04-06
WO2006036735A3 true WO2006036735A3 (fr) 2008-01-24

Family

ID=36119422

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/033964 WO2006036735A2 (fr) 2004-09-22 2005-09-22 Procede de detection et de quantification de multiples composants subcellulaires

Country Status (6)

Country Link
EP (1) EP1791979A4 (fr)
JP (1) JP2008518587A (fr)
CN (1) CN101228283A (fr)
AU (1) AU2005289765A1 (fr)
CA (1) CA2580961A1 (fr)
WO (1) WO2006036735A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107267628A (zh) * 2017-07-13 2017-10-20 苏州贝康医疗器械有限公司 胚胎植入前染色体异常检测试剂盒

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2724563C (fr) * 2008-05-16 2018-07-17 Huron Technologies International Inc. Systeme d'imagerie avec optimisation de la plage dynamique
EP2519825B1 (fr) * 2009-12-31 2015-01-14 Ventana Medical Systems, Inc. Détection simultanée de l'état de mutation et du nombre de copies d'un gène
KR20140120321A (ko) * 2012-02-01 2014-10-13 벤타나 메디컬 시스템즈, 인코포레이티드 조직 샘플들에 있어서의 유전자들을 검출하는 시스템
JP6231709B1 (ja) * 2016-05-31 2017-11-15 シスメックス株式会社 蛍光画像分析装置および分析方法
JP6959755B2 (ja) 2017-04-14 2021-11-05 シスメックス株式会社 蛍光画像分析装置、蛍光画像の分析方法及びコンピュータプログラム
JP6948145B2 (ja) 2017-04-14 2021-10-13 シスメックス株式会社 蛍光画像分析装置、蛍光画像の画像処理方法及びコンピュータプログラム
CN108956431B (zh) * 2018-08-09 2021-04-30 苏州叠代生物科技有限公司 一种单采血小板制品中残留细胞的检测方法
JP7376245B2 (ja) 2019-03-29 2023-11-08 シスメックス株式会社 蛍光画像分析装置及び蛍光画像分析方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136540A (en) * 1994-10-03 2000-10-24 Ikonisys Inc. Automated fluorescence in situ hybridization detection of genetic abnormalities
US6169816B1 (en) * 1997-05-14 2001-01-02 Applied Imaging, Inc. Identification of objects of interest using multiple illumination schemes and finding overlap of features in corresponding multiple images

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BIANCHI ET AL.: "Detection of fetal cells with 47, XY, +21 karyotype in maternal peripheral blood", HUMA. GENET., vol. 90, 1992, pages 368 - 370, XP001069259 *
OERTHER ET AL.: "Simultaneous oligonucleotide probe hybridization and immunostaining for in situ detection of Gordona species in activated sludge", FEMS MICROBIOLOGY ECOLOGY, vol. 29, no. 2, 1999, pages 129 - 136, XP003022483 *
See also references of EP1791979A4 *
WAGGONER ET AL.: "Multiparameter Fluorescence Imaging Microscope: Reagents and Instruments", HUMAN PATHOLOGY, vol. 27, no. 5, May 1996 (1996-05-01), pages 494 - 502, XP000920886 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107267628A (zh) * 2017-07-13 2017-10-20 苏州贝康医疗器械有限公司 胚胎植入前染色体异常检测试剂盒

Also Published As

Publication number Publication date
EP1791979A4 (fr) 2009-07-29
JP2008518587A (ja) 2008-06-05
AU2005289765A1 (en) 2006-04-06
CA2580961A1 (fr) 2006-04-06
EP1791979A2 (fr) 2007-06-06
WO2006036735A2 (fr) 2006-04-06
CN101228283A (zh) 2008-07-23

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