WO2006015810A3 - Fluoreszenz-basierte assays zur schnellen quantitativen analyse von bimolekülen (proteine und nukleinsäuren) durch anreicherung auf zellen oder beads - Google Patents

Fluoreszenz-basierte assays zur schnellen quantitativen analyse von bimolekülen (proteine und nukleinsäuren) durch anreicherung auf zellen oder beads Download PDF

Info

Publication number
WO2006015810A3
WO2006015810A3 PCT/EP2005/008508 EP2005008508W WO2006015810A3 WO 2006015810 A3 WO2006015810 A3 WO 2006015810A3 EP 2005008508 W EP2005008508 W EP 2005008508W WO 2006015810 A3 WO2006015810 A3 WO 2006015810A3
Authority
WO
WIPO (PCT)
Prior art keywords
beads
molecule
detected
biomolecules
fluorescence
Prior art date
Application number
PCT/EP2005/008508
Other languages
English (en)
French (fr)
Other versions
WO2006015810B1 (de
WO2006015810A2 (de
Inventor
Marina Sauer
Original Assignee
Diacdem Chip Technology Gmbh
Marina Sauer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diacdem Chip Technology Gmbh, Marina Sauer filed Critical Diacdem Chip Technology Gmbh
Publication of WO2006015810A2 publication Critical patent/WO2006015810A2/de
Publication of WO2006015810A3 publication Critical patent/WO2006015810A3/de
Publication of WO2006015810B1 publication Critical patent/WO2006015810B1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms

Abstract

Die vorliegende Erfindung betrifft ein Verfahren zur Detektion mindestens eines Moleküls (212) in einer Lösung in einem Reak­tionsreservoir eines Mikrofluidik-Chips mit einem Boden, wobei a) das zu detektierende Molekül mit mit geeigneten Antikörpern beschichteten Kügelchen (210) und mit farbstoffmarkierten Se­kundärantikörpern (214) in Kontakt gebracht wird, b) wobei es sich bei dem farbstoffmarkierten Sekundärantikörper (214) bevorzugt um einen Antikörper handelt, der durch die Bin­dung an das zu detektierende Molekül fluoreszenzfähig wird, c) wobei das zu detektierende Molekül mit den genannten Sub­stanzen bindet, d) wobei sich ein Komplex (216) aus zu detektierendem Molekül, Kügelchen und farbstoffmarkierten Sekundärantikörpern bildet, und e) wobei dieser Komplex sedimentiert und am Boden nachgewiesen wird.
PCT/EP2005/008508 2004-08-06 2005-08-05 Fluoreszenz-basierte assays zur schnellen quantitativen analyse von bimolekülen (proteine und nukleinsäuren) durch anreicherung auf zellen oder beads WO2006015810A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102004038163.1 2004-08-06
DE200410038163 DE102004038163A1 (de) 2004-08-06 2004-08-06 Fluoreszenz-basierte Assays zur schnellen, quantitativen Analyse von Biomolekülen (Proteine und Nukleinsäuren) durch Anreicherung auf Zellen oder Beads

Publications (3)

Publication Number Publication Date
WO2006015810A2 WO2006015810A2 (de) 2006-02-16
WO2006015810A3 true WO2006015810A3 (de) 2006-08-24
WO2006015810B1 WO2006015810B1 (de) 2006-09-28

Family

ID=35355030

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/008508 WO2006015810A2 (de) 2004-08-06 2005-08-05 Fluoreszenz-basierte assays zur schnellen quantitativen analyse von bimolekülen (proteine und nukleinsäuren) durch anreicherung auf zellen oder beads

Country Status (2)

Country Link
DE (1) DE102004038163A1 (de)
WO (1) WO2006015810A2 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1977829A1 (de) * 2007-03-29 2008-10-08 Roche Diagnostics GmbH Vorrichtung zur parallelen Durchführung mehrerer Analysen
CN114910460A (zh) * 2022-05-22 2022-08-16 北京华牛世纪生物技术研究院 一种基于适配体的蛋白质芯片及其制备与应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136543A (en) * 1997-01-31 2000-10-24 Hitachi, Ltd. Method for determining nucleic acids base sequence and apparatus therefor
WO2000063437A2 (en) * 1999-04-20 2000-10-26 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
EP1231282A2 (de) * 1990-12-06 2002-08-14 Affymetrix, Inc. Verfahren und Zussamensetzungen für den Nachweis von Polymeren
US20030148544A1 (en) * 2001-06-28 2003-08-07 Advanced Research And Technology Institute, Inc. Methods of preparing multicolor quantum dot tagged beads and conjugates thereof
WO2004066210A1 (en) * 2003-01-22 2004-08-05 Cyvera Corporation Hybrid random bead/chip based microarray

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4486530A (en) * 1980-08-04 1984-12-04 Hybritech Incorporated Immunometric assays using monoclonal antibodies
FI842992A0 (fi) * 1984-07-26 1984-07-26 Labsystems Oy Immunologiskt definitionsfoerfarande.
US4859612A (en) * 1987-10-07 1989-08-22 Hygeia Sciences, Inc. Metal sol capture immunoassay procedure, kit for use therewith and captured metal containing composite
IL138286A (en) * 1998-03-11 2004-02-19 Steag Micro Parts Gmbh Sample support
ATE270344T1 (de) * 1999-11-16 2004-07-15 Atto Tec Gmbh Farbstoffmarkiertes oligonukleotid zum markieren eines nukleinsäuremoleküls
DE10117430A1 (de) * 2001-04-06 2002-10-10 Nicole Marme Hochempfindlicher und hochspezifischer Enzymnachweis mit einer Nachweisgrenze bis in den femtomolaren Bereich
DE10137288A1 (de) * 2001-08-01 2003-02-20 Markus Sauer Nachweis humaner Auto-Antikörper

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1231282A2 (de) * 1990-12-06 2002-08-14 Affymetrix, Inc. Verfahren und Zussamensetzungen für den Nachweis von Polymeren
US6136543A (en) * 1997-01-31 2000-10-24 Hitachi, Ltd. Method for determining nucleic acids base sequence and apparatus therefor
WO2000063437A2 (en) * 1999-04-20 2000-10-26 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20030148544A1 (en) * 2001-06-28 2003-08-07 Advanced Research And Technology Institute, Inc. Methods of preparing multicolor quantum dot tagged beads and conjugates thereof
WO2004066210A1 (en) * 2003-01-22 2004-08-05 Cyvera Corporation Hybrid random bead/chip based microarray

Also Published As

Publication number Publication date
WO2006015810B1 (de) 2006-09-28
DE102004038163A1 (de) 2006-03-16
WO2006015810A2 (de) 2006-02-16

Similar Documents

Publication Publication Date Title
US20200096502A1 (en) Analyte Detection
US6846638B2 (en) Method and system for rapid biomolecular recognition of amino acids and protein sequencing
Wang et al. Ultrasensitive microfluidic solid-phase ELISA using an actuatable microwell-patterned PDMS chip
US5989924A (en) Device for determining an analyte in a sample
US20100311186A1 (en) Devices and methods for performing receptor binding assays using magnetic particles
Kawabata et al. “Electrokinetic Analyte Transport Assay” for α‐fetoprotein immunoassay integrates mixing, reaction and separation on‐chip
EP2248912B8 (de) Hochempfindliche Assays für die Pathogendetektion unter Verwendung der metallverstärkten Fluoreszenz
WO2006074482A3 (en) Methods and kits for strand-specific polynucleotide detection with cationic multichromophores
JP2000516343A (ja) キャピラリー電気泳動酵素イムノアッセイ
WO2002079490A3 (en) Bio-barcodes based on oligonucleotide-modified particles
BRPI0610092B8 (pt) métodos para a detecção de um analito e de um ácido nucleico em uma amostra, e de sintetização de uma molécula de ácido nucleico ou de análogo de ácido nucleico, kit reagente, uso do mesmo, molécula de ácido nucleico ou de análogo de ácido nucleico e produto associado
WO2004027388A3 (en) Novel green and orange fluorescent labels and their uses
Dunbar et al. Microsphere-based multiplex immunoassays: development and applications using Luminex® xMAP® technology
KR101263398B1 (ko) 다중 분석을 위한 원심력 기반 미세 유동장치 및 이를 이용한 분석 방법
US20110116972A1 (en) Microfluidic assays and microfluidic devices
US20070238140A1 (en) Method For Multiplex Bead-Based Assays Using Chemiluminescence and Fluorescence
WO2006071970A3 (en) Methods for the detection of analytes in a sample
WO2006015810A3 (de) Fluoreszenz-basierte assays zur schnellen quantitativen analyse von bimolekülen (proteine und nukleinsäuren) durch anreicherung auf zellen oder beads
JPWO2007122943A1 (ja) 免疫学的測定法およびチップ
US7846713B2 (en) Calibrating microarrays
EP2282206A3 (de) Verfahren und Vorrichtung zur Bestimmung mehrerer Analyten mit simultaner internen Kontrolle in einer grafischen Kombination
WO2000034790A3 (de) Verfahren zum nachweis von antikörpern oder antigenen sowie zur blutgruppenbestimmung
EP1127166A1 (de) Methode und system zur amplizierten array analyse
WO2004076684A3 (en) Compositions and methods for the detection of mammalian muscle troponin i
EP3505934A1 (de) Sensorkörper zur bindung und/oder anreicherung und/oder detektion eines analyten in einer probe

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KM KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NG NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SM SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU LV MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DPE1 Request for preliminary examination filed after expiration of 19th month from priority date (pct application filed from 20040101)
NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase