WO2006012366A3 - Methods for making and using recombinant bacillus thuringiensis spores - Google Patents

Methods for making and using recombinant bacillus thuringiensis spores Download PDF

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Publication number
WO2006012366A3
WO2006012366A3 PCT/US2005/025788 US2005025788W WO2006012366A3 WO 2006012366 A3 WO2006012366 A3 WO 2006012366A3 US 2005025788 W US2005025788 W US 2005025788W WO 2006012366 A3 WO2006012366 A3 WO 2006012366A3
Authority
WO
WIPO (PCT)
Prior art keywords
recombinant
methods
bacillus thuringiensis
spores
strains
Prior art date
Application number
PCT/US2005/025788
Other languages
French (fr)
Other versions
WO2006012366A2 (en
Inventor
Stanley Goldman
John Libs
Original Assignee
Phyllom Llc
Stanley Goldman
John Libs
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Phyllom Llc, Stanley Goldman, John Libs filed Critical Phyllom Llc
Priority to EP05795245A priority Critical patent/EP1778714A4/en
Publication of WO2006012366A2 publication Critical patent/WO2006012366A2/en
Publication of WO2006012366A3 publication Critical patent/WO2006012366A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Pest Control & Pesticides (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Agronomy & Crop Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention provides isolated nucleic acid and amino acid sequences for Bacillus thuringiensis (Bt) spore outer coat protein and exosporium protein genes fusion proteins containing such proteins. The invention further includes methods for using the genes to construct expression cassettes, expression vectors, and recombinant Bt strains that have exogenous proteins attached to their spores. The exogenous proteins attached to the surface of recombinant Bt spores may provide insecticidal, enzymatic, or antigenic activity. The invention also provides for compositions made from the recombinant Bt strains and methods for fermenting and using the recombinant Bt strains produced by the invention.
PCT/US2005/025788 2004-07-20 2005-07-20 Methods for making and using recombinant bacillus thuringiensis spores WO2006012366A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP05795245A EP1778714A4 (en) 2004-07-20 2005-07-20 Methods for making and using recombinant bacillus thuringiensis spores

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US58898204P 2004-07-20 2004-07-20
US60/588,982 2004-07-20
US59114204P 2004-07-27 2004-07-27
US60/591,142 2004-07-27

Publications (2)

Publication Number Publication Date
WO2006012366A2 WO2006012366A2 (en) 2006-02-02
WO2006012366A3 true WO2006012366A3 (en) 2006-09-28

Family

ID=35786687

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/025788 WO2006012366A2 (en) 2004-07-20 2005-07-20 Methods for making and using recombinant bacillus thuringiensis spores

Country Status (2)

Country Link
EP (1) EP1778714A4 (en)
WO (1) WO2006012366A2 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102408472A (en) * 2011-11-29 2012-04-11 四川农业大学 Bt (Bacillus Thuringiensis) protein Cry62Aa1 and coding gene as well as application thereof
CN102408474A (en) * 2011-12-07 2012-04-11 四川农业大学 Bt protein Cry69Aa1, and coding gene and application thereof
CN102417538A (en) * 2011-12-07 2012-04-18 四川农业大学 Bt protein Cry68Aa1 and encoding gene and application thereof
CN102408473B (en) * 2011-11-29 2013-06-12 四川农业大学 Bt (Bacillus Thuringiensis) protein Cyt3Aa1 and coding gene as well as application thereof
CN102408475B (en) * 2011-12-07 2013-06-12 四川农业大学 Bt protein Cryt1Da1, and coding gene and application thereof

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0504940D0 (en) * 2005-03-10 2005-04-20 Secr Defence Vaccine formulation
US9133251B2 (en) 2008-02-22 2015-09-15 The Curators Of The University Of Missouri Bacillus based delivery system and methods of use
WO2012090789A1 (en) * 2010-12-28 2012-07-05 国立大学法人広島大学 Polypeptide for distinguishing silicon oxide from silicon nitride, and use thereof
AU2013337422A1 (en) * 2012-11-02 2015-05-07 Genesys Research Institute Compositions and methods for auditory therapy
US9403881B2 (en) * 2013-03-14 2016-08-02 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods of use
US9573980B2 (en) * 2013-03-15 2017-02-21 Spogen Biotech Inc. Fusion proteins and methods for stimulating plant growth, protecting plants from pathogens, and immobilizing Bacillus spores on plant roots
BR112017005509A2 (en) 2014-09-17 2018-08-14 Bayer Cropscience Lp compositions comprising recombinant bacillus cells and another biological control agent.
WO2016044533A1 (en) 2014-09-17 2016-03-24 Bayer Cropscience Lp Compositions comprising recombinant bacillus cells and a fungicide
US20170295798A1 (en) * 2014-09-17 2017-10-19 Bayer Cropscience Lp Compositions comprising recombinant bacillus cells and a fungicide
AR101959A1 (en) 2014-09-17 2017-01-25 Bayer Cropscience Lp COMPOSITIONS THAT INCLUDE BACILLUS RECOMBINATING CELLS AND AN INSECTICIDE
WO2016044542A1 (en) * 2014-09-17 2016-03-24 Bayer Cropscience Lp Compositions comprising recombinant bacillus cells and an insecticide
BR122023020858A2 (en) 2014-09-17 2024-01-30 Spogen Biotech Inc PLANT SEED COATED WITH A RECOMBINANT MICROORGANISM THAT EXPRESSES AN ENZYME THAT CATALYZES THE PRODUCTION OF NITRIC OXIDE
KR102179224B1 (en) * 2014-09-17 2020-11-16 바이엘 크롭사이언스 엘피 Compositions comprising recombinant bacillus cells and another biological control agent
PE20170942A1 (en) * 2014-10-16 2017-07-13 Monsanto Technology Llc CRY1DA1 AMINO ACID SEQUENCE VARIANTS PROTEINS ACTIVE FOR LEPIDOPTERS
BR112018068719A2 (en) * 2016-03-16 2019-01-22 Spogen Biotech Inc Methods for Producing an Immunogenic Response in an Aquatic Animal and Pharmaceutical Composition
IL295513B1 (en) 2016-03-16 2024-04-01 Spogen Biotech Inc Methods for promoting plant health using free enzymes and microorganisms that overexpress enzymes
AU2017342921B2 (en) * 2016-10-10 2023-06-15 Monsanto Technology Llc Novel insect inhibitory proteins
AR113123A1 (en) * 2017-09-20 2020-01-29 Spogen Biotech Inc FUSION PROTEINS, RECOMBINANT BACTERIA AND EXOSPORIUM FRAGMENTS TO PROMOTE PLANT HEALTH
JP2021508235A (en) 2017-11-16 2021-03-04 バイエル クロップサイエンス エルピーＢａｙｅｒ Ｃｒｏｐｓｃｉｅｎｃｅ Ｌｐ Endogenous spore display platform, products and methods based on Paenibacillus
TWI728264B (en) * 2018-09-10 2021-05-21 高雄醫學大學 A microbial agent for controlling transmission of mosquito-borne diseases
CN111440814A (en) * 2020-02-26 2020-07-24 中国农业科学院作物科学研究所 Insect-resistant fusion gene mCry1AbVip3A, expression vector and application thereof
WO2023137458A1 (en) * 2022-01-14 2023-07-20 Syngenta Crop Protection Ag Compositions and methods for screening insecticidal proteins
CN116514936B (en) * 2023-06-29 2023-09-29 莱肯生物科技(海南)有限公司 Insect-resistant protein and preparation method and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020150594A1 (en) * 2000-06-26 2002-10-17 Maxygen, Inc. Methods and compositions for developing spore display systems for medicinal and industrial applications
US20030165538A1 (en) * 2000-06-26 2003-09-04 Maxygen Incorporated Methods and compositions for developing spore display systems for medicinal and industrial applications

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5766914A (en) * 1995-01-26 1998-06-16 Michigan State University Method of producing and purifying enzymes
US5800821A (en) * 1995-03-10 1998-09-01 New England Medical Center Hospitals, Inc. Bacterial spores as a heat stable vaccine delivery system

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020150594A1 (en) * 2000-06-26 2002-10-17 Maxygen, Inc. Methods and compositions for developing spore display systems for medicinal and industrial applications
US20030165538A1 (en) * 2000-06-26 2003-09-04 Maxygen Incorporated Methods and compositions for developing spore display systems for medicinal and industrial applications

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BRAVO ET AL.: "Analysis of cry1Aa expression in sigE and sigK mutants of Bacillus thuringiensis", MOLECULAR AND GENERAL GENETICS, vol. 250, no. 6, 10 April 1996 (1996-04-10), pages 734 - 741, XP002007697 *
CRICKMORE ET AL.: "Use of an Operon Fusion to Induce Expression and Crystallisation of a Bacillu Thuringiensis delta-Endotoxin Encoded by a Cryptic Gene", MOLECULAR AND GENERAL GENETICS, vol. 242, 1994, pages 365 - 368, XP008081535 *
NAIMOV ET AL.: "Bacillus thuringiensis delta-Endotoxin Cry1 Hybrid Proteins with Increased Activity against the Colorado Potato Beetle", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 67, no. 11, November 2001 (2001-11-01), pages 5328 - 5330, XP003013551 *
VAN DER SALM ET AL.: "Insect Resistance of Transgenic Plants that Express Modified Bacillus thuringiensis cry1A(b) and cry1C Genes: A resistence Management Strategy", PLANT MOLECULAR BIOLOGY, vol. 25, no. 1, 1994, pages 51 - 59, XP001029215 *
WEBB ET AL.: "Use of Green Fluorescent Protein for Visualization of Cell-Specific Gene Expression and Subcellular Protein Localization during Sporulation in Bacillus subtilis", JOURNAL OF BACTERIOLOGY, vol. 177, no. 20, October 1995 (1995-10-01), pages 5906 - 5911, XP002089513 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102408472A (en) * 2011-11-29 2012-04-11 四川农业大学 Bt (Bacillus Thuringiensis) protein Cry62Aa1 and coding gene as well as application thereof
CN102408473B (en) * 2011-11-29 2013-06-12 四川农业大学 Bt (Bacillus Thuringiensis) protein Cyt3Aa1 and coding gene as well as application thereof
CN102408474A (en) * 2011-12-07 2012-04-11 四川农业大学 Bt protein Cry69Aa1, and coding gene and application thereof
CN102417538A (en) * 2011-12-07 2012-04-18 四川农业大学 Bt protein Cry68Aa1 and encoding gene and application thereof
CN102408475B (en) * 2011-12-07 2013-06-12 四川农业大学 Bt protein Cryt1Da1, and coding gene and application thereof
CN102417538B (en) * 2011-12-07 2013-06-19 四川农业大学 Bt protein Cry68Aa1 and encoding gene and application thereof

Also Published As

Publication number Publication date
EP1778714A2 (en) 2007-05-02
WO2006012366A2 (en) 2006-02-02
EP1778714A4 (en) 2008-05-21

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