WO2006011280A1 - Procede et agent de purification environnementaux - Google Patents

Procede et agent de purification environnementaux Download PDF

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Publication number
WO2006011280A1
WO2006011280A1 PCT/JP2005/008336 JP2005008336W WO2006011280A1 WO 2006011280 A1 WO2006011280 A1 WO 2006011280A1 JP 2005008336 W JP2005008336 W JP 2005008336W WO 2006011280 A1 WO2006011280 A1 WO 2006011280A1
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Prior art keywords
environmental purification
genus
environmental
strain
bacteria
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PCT/JP2005/008336
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English (en)
Japanese (ja)
Inventor
Kiyoki Komori
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Komori Bio Laboratory Inc.
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Priority to PCT/JP2005/013443 priority Critical patent/WO2006011416A1/fr
Publication of WO2006011280A1 publication Critical patent/WO2006011280A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

Definitions

  • the present invention relates to an environmental purification method, an environmental purification agent used in the method, and bacteria that can be used in these purification methods and environmental purification agents.
  • physicochemical treatment and biological treatment are broadly used to treat domestic wastewater and wastewater from factories.
  • physicochemical treatments such as agglomeration, precipitation, filtration and adsorption are excellent in processing speed.
  • biological treatment is not efficient for wastewater containing a high concentration of pollutants, and it takes much time. Because of its superiority, biological treatment methods are often adopted.
  • bacteria that can degrade phosphorus compounds have not been found so far, and it has been thought that biological treatment cannot reduce phosphorus compounds in wastewater.
  • biological treatment cannot reduce phosphorus compounds in wastewater.
  • the search for bacteria that can treat pollutants efficiently is one of the major themes.
  • Odor generation is also a problem. In particular, it is necessary to quickly reduce amine-based compounds and sulfur-based compounds that cause bad odors in fetch-type toilets in areas without sewage facilities and toilets in trains that must be installed close to passenger seats. There is.
  • Japanese Patent Application Laid-Open No. 11-1137953 discloses a deodorizing apparatus including a carrier supporting microorganisms, a watering facility for spraying water on the carrier, and a water storage tank for collecting the water sprayed and having nitrifying bacteria. It is disclosed.
  • Japanese Patent Application Laid-Open No. 2001-70424 discloses a method for producing a biological deodorant carrying bacteria belonging to the genus Baci 11 1us such as Bacillusthuringiensis species. In the examples, specific treatments such as ammonia are disclosed. Data are also disclosed. However, as is clear from the attached diagram, the technology is to treat volatile compounds in the air, and to treat substances that directly cause odors. is not.
  • JP-A-6-190028 and JP-A-10-248556 there are a deodorant containing bacteria belonging to the genus C lostri du im and the genus Thermos and a deodorizer containing bacteria belonging to the genus Pseud omonas, respectively.
  • An experimental example is disclosed in which methyl sulfide and methyl disulfide, which are malodorous substances, are treated.
  • Examples of familiar environmental pollution include “numeric” that occurs in kitchen drains and drains, bathroom drains, bathtubs, toilet flush tanks, and water tanks.
  • the true nature of this slime is a polysaccharide produced mainly by various germs, molds and algae. Therefore, a compound having a bactericidal action is used for suppressing slime.
  • fungicides can be slow and can be dangerous to use at home.
  • the problem to be solved by the present invention is to use an environmental purification method for treating harmful organic compounds and inorganic compounds by bacteria, an environmental purification agent used in the method, and these environmental purification method and environmental purification agent. It is to provide bacteria that can.
  • the present inventors have searched for bacteria useful for environmental purification, and the Emp edobacter genus bacteria that have never been used for environmental purification so far have only COD, malodorous components, etc. of wastewater.
  • the present invention was completed by finding that it has the ability to reduce the concentration of phosphorus compounds.
  • the environmental purification method of the present invention is characterized in that the bacteria belonging to the genus Empedobacter and the culture thereof are brought into contact with the treatment object.
  • the environmental purification agent of the present invention is characterized in that it contains bacteria belonging to the genus Empedobacter and / or a culture thereof.
  • the Emp e do b a c t er genus bacteria exhibiting particularly excellent effects with the environmental purification method and the environmental purification agent according to the present invention are S I I D 2926-1b strain (FERM A).
  • Fig. 1 Chromatographic results showing the fatty acid composition of S I ID 2926-1b strain.
  • Fig. 2 SIID 2926-1 is a molecular phylogenetic tree containing strain b and its related strains. BEST MODE FOR CARRYING OUT THE INVENTION
  • the strain S 1 I D 2 926-1b is deposited at the Patent Microorganism Deposit Center (NPMD) under the deposit number: FER M AP-20108.
  • SIID 2926-1b cell morphology, gram staining, spore presence, flagellar motility, and nu trientagar (Oxoid) observed with an optical microscope (Olympus, U-LH1000) Morphology of colonies cultured at 30 ° C for 24 hours, as well as B arrow, G. I. And Felth am, RKA, Cowandand Steel 's Man nu alforthe I dentiiication of Medical B acteri a. 3 rdedition Table 1 shows the test results of catalase reaction, oxidase reaction opi-glucose oxidation / fermentation (OZF) according to, C am bridge University Pres.
  • Table 2 shows the results of biochemical property tests using the API system (manufactured by BI OMER I EUX), which is a reagent for testing bacterial identification.
  • the strain SIID 2926-1b is similar to the fungal group classified in the F 1 avobacteriaceae family, but the genus species has not been identified. However, from the results of the examples described later, it can be concluded that the strain SIID 2926-1b is a bacterium classified into the genus Em pedobacter in the family F 1 a V obacteriaceae.
  • SII D2926-1b strain reduces COD, BOD, nitrogen compounds and phosphorus compounds in wastewater, suppresses the generation of amine compounds and sulfur compounds that cause foul odors, and suppresses polysaccharides that cause waste. Has an excellent action. Therefore, other bacteria belonging to the genus Empedobacter are also considered to have these effects and can be used for environmental purification in the same manner as the SIID2926-1b strain. Also, a plurality of bacteria belonging to the genus Empe dobacter may be used in combination.
  • the environmental purification method of the present invention is characterized by bringing the bacteria belonging to the genus Empedobacter and / or the culture thereof into contact with the treatment object.
  • harmful compounds in the environment especially organic compounds that cause eutrophication of rivers, nitrogen compounds, phosphorus compounds; amine compounds and sulfur compounds that cause bad odors; The generation of molds can be suppressed.
  • the bacteria belonging to the genus Empedobacter used in the method of the present invention are as described above.
  • Emp edobacter bacteria culture is Emp edobact It includes a medium used for culturing er genus bacteria and a compound, enzyme, etc. released into the medium with the growth of Emp edobacter genus bacteria.
  • Empedobacter bacteria themselves can degrade harmful compounds, but enzymes produced by bacteria may have similar effects.
  • the medium is not particularly limited as long as it is suitable for cultivation of bacteria belonging to the genus Empedobacter.
  • a carbon nutrient source such as sugar, a nitrogen nutrient source such as urea or peptone, or a trace element source such as yeast extract
  • water such as pure water, purified water, or distilled water can be used.
  • the object to be treated of the method of the present invention is not particularly limited as long as it contains a harmful compound or generates a harmful compound.
  • Examples include wastewater that causes eutrophication of rivers and other wastewater such as domestic wastewater and food factory wastewater, odor sources such as manure and livestock wastewater, food waste such as raw garbage, and microbial sludge.
  • toilet rooms where bad odor is a problem, toilet bowl, dog and cat manure, ashtray, garbage container; wastewater, mold, bathroom drains, drains, walls, bathtubs, toilet tanks Emperedobacter bacteria and / or their cultures may be directly applied to water tanks, outdoor units of air conditioners, garbage containers, etc.
  • the environmental purification agent used in the method of the present invention is characterized in that it contains bacteria belonging to the genus Empedobacter and / or a culture thereof.
  • the environmental cleansing agent of this effort makes harmful compounds in the environment, especially organic compounds that cause eutrophication of rivers, nitrogen compounds, phosphorus compounds; amine compounds and sulfur compounds that cause bad odors; It is possible to suppress polysaccharides that cause waste. Therefore, the environmental purification agent of the present invention is useful as a wastewater treatment agent, malodor inhibitor or deodorizer, slime inhibitor or polysaccharide generation inhibitor, mold generation inhibitor, and the like.
  • the environmental purification agent of the present invention may be one in which the Empedo bacter bacterium and / or its culture is directly administered to the treatment object, but may be in a form corresponding to the type of the treatment object.
  • the bacteria belonging to the genus Empedobacter and / or its culture may be dispersed in a solvent and sprayed onto the object to be treated.
  • Solvents that can be used in this case include water; alcohols such as ethanol; mixed solvents of water and alcohols.
  • concentration of the liquid is not particularly limited.
  • the solution may be about 5000 to 10000 times by mass added to Empedobacter bacteria.
  • pH adjusters, pigments, disinfectants, etc. may be used in combination.
  • This dispersion shall also contain a culture solution of Empedobacter bacteria.
  • the bacteria belonging to the genus Empedobacter and / or the culture thereof may be put into a conventional wastewater treatment facility such as a septic tank. In that case, it is not particularly necessary when the culture is introduced.
  • a nutrient source suitable for the growth of the bacterium such as a carbon source, a nitrogen source, and a trace element source, is used. Add other conditions and make sure that the other conditions are met. Plant-based residues may be added as a nutrient source. This is because plant residues are not only excellent as a nutrient source for bacteria, but can also be reused for what should be discarded.
  • plant residues examples include rice bran, okara, sawdust, coffee lees, oil lees, soybean lees, pears, sugarcane bagasse, etc.
  • rice lees and coffee lees Is the best.
  • a product obtained by culturing the bacterium of the present invention in the above-described plant residue is useful as it can be used for wastewater treatment as it is.
  • the preparation method is, for example, adding the bacterium of the present invention and other nutrients to the plant residue, giving sufficient water, incubating at about 37-39 ° C, and fermenting for several days. This can be added to the workpiece as it is, or a dried product can be added to the workpiece.
  • the environmental purification agent of the present invention may be one in which an Empedobacter genus Opino or a culture thereof is supported on a carrier.
  • the carrier that can be used include one or more selected from the group consisting of rice bran, coffee koji, soybean koji, coconut shell, activated carbon, porous silica, gauze, non-woven fabric, and fabric.
  • This environmental purification agent can be produced by, for example, adding a culture solution of bacteria belonging to the genus Empe dobacter to a carrier and drying it appropriately.
  • the addition amount of the environmental purification agent bacterium according to the present invention may be appropriately adjusted according to, for example, the COD of wastewater, the degree of malodor, the presence of solid components such as garbage.
  • the material to be treated is wastewater, generally about 0.3 mg of bacterial cell weight is added to 1 L of wastewater. Add it.
  • the object to be treated is a toilet room or garbage, spread the environmental cleansing agent, which is a dispersion of Empedobacter bacteria and / or their cultures, to the extent that no foul odors occur.
  • the strain SII D2926-1b grows at 30 ° C and 37 ° C as described above, so it is not necessary to control the temperature, and it can be treated at room temperature. May be incubated at an appropriate temperature. Specific treatment conditions may be determined by preliminary experiments.
  • the present invention will be described more specifically with reference to examples. However, the present invention is not limited by the following examples, and appropriate modifications are made within a range that can meet the purpose described above and below. These are all included in the technical scope of the present
  • Genomic DNA was extracted from the cells of S I I D2926—lb strain (FERM AP—20108).
  • Insta GeneMatrix manufactured by BIORAD
  • BIORAD BIORAD
  • a region of about 500 bp on the 5 ′ end side of 16 S rDNA was amplified by PCR. Thereafter, the amplified base sequence was sequenced to determine the base sequence.
  • PCR and cycle sequences use the Micro S eq 500 16 S rDNA B acterial Identification PCR Kit (Applied Biosystems), and the Gene Recycler Gene Amp PCR System 9600 (Ap 1 ied B iosyst ems) and AB IPR I SM 3100 DNA Sequencer (App 1 ied B iosyst ems) were used as the DNA sequencer.
  • the basic operation from PCR to cyclosequencing was in accordance with the protocol (P / N4346296 Rev. B) of Ap p lie e Bios stems.
  • genus species were identified using the obtained base sequence information.
  • a database of reference strains for each genus species Micro S eq B acterial 500 0 Library v. 0023 (Ap plied B iosyst ems)
  • the homology rate with known bacteria was determined using MicroSeq Microbial Identification System Vs. 1.4.1 as the analysis software and BLAST as the algorithm. Table 3 shows the results of the top 5 homology ratios.
  • the homology with Emp edobacterbrevis is 95%, and it can be said that the SIID 2926-1b strain does not belong to the Emp edobacterbrevis species. Some of the homology was high, but these were not the reference strains. In addition, even if the homology of the entire base sequence of 16 S r DNA is 100%, it does not necessarily prove that the strain is the same.
  • S I I D 2926—1b strain was inoculated on a plate medium of T r i p t i c a c e S o y (S CD) A g a r (manufactured by B c t o n D i c k i in so) and cultured at 30 ° C. for 24 hours.
  • the cells of 4 plates were scraped with a cell scraper and rubbed against the bottom of a 13 mL glass vial.
  • Extract solution (mixed solution of 20 OmL of hexane and 200 mL of t-butyl methyl ether) 1. 25 mL was added to the sample solution, stirred for 10 minutes with a shaker, and allowed to stand. The aqueous layer separated into two layers was removed with a Pasteur pipette. To the remaining organic layer was added 3 mL of a washing solution (10.8 g of sodium hydroxide and 90 OmL of distilled water uniformly mixed) and stirred for 5 minutes with a shaker. As a result, a few drops of saturated saline were added. The two layers were separated. The upper 2 3 of the separated organic layer was taken out with a Pasteur pipette and transferred to a gas chromatography sample pail to obtain a measurement sample.
  • the fatty acid composition of the sample was measured using a gas chromatography system HP 6890 (manufactured by Hewlett-Packard). The results are shown in Figure 1. The In addition, a homology search with known bacterial species was performed on this result using the Sherlock Microdental Identification System. The results are shown in Table 5.
  • “Sum InFeature 3” is either C 16: 1 w7 c or C 15: 0 i so 2 OH, whose retention times are almost the same.
  • the “similarity” is 1.000 when the bacterial species have the same composition, and if the value is 0.5 or more as a guide, it is similar to the fatty acid composition of the searched bacterial species. However, at 0.30 or less, it is considered that there are no bacterial species with similar fatty acid composition.
  • alignment software C lustal X (Thomson, JD et al., “T he C 1 ust 1 X wind ow sinterface: flexible strategy for multiple sequencealig nme ntaided by qualityana 1 ysistools ”, Nucleic Acids Re serach, 24, p p. 4876-4882 (1 997)).
  • -Software MEG A 2 (Kumar, R. et al., “MEGA 2: Molecular Evo- lutionary Genetics Ana 1 ysissoft wa re”, Bioinfo rma tics, 1 7 pp.
  • SIID 2926-1 b strain, coffee koji, and urea to rice bran, incubate groundwater sprayed at 37-39 ° C for 5 days, and then air dry moderately to obtain SIID 2926-1 b A plant-based residue containing the strain was created.
  • 10 L of raw wastewater treatment plant water was taken into a 12 L sealed vacuum desiccator, and the plant residue was added at a rate of 10 g per 1 L of the raw water (total 100 g). The desiccator was sealed and incubated at 25 ° C with shaking.
  • the SIID 29 2 6-1b strain of the present invention can reduce the concentration of ammonia, which causes malodor from general raw manure water, to almost half in one hour.
  • the ammonia concentration was maintained at a low level. Therefore, it was demonstrated that the S I I D 2 9 2 6-1b strain of the present invention has a high deodorizing ability.
  • the wastewater discharge flow: 25 m 3 / day
  • the food kamaboko
  • discharge flow 25 m 3 / day
  • pH chemical oxygen demand
  • biochemical oxygen demand Amount suspended solid content
  • total nitrogen content total phosphorus content
  • n-hexane extractables mineral oils and animal and plant fats and oils
  • Units for numerical values other than pH are all mg / L.
  • the SIID 2 9 2 6-1 b strain of the present invention has a chemical oxygen demand (C OD), biochemical oxygen demand (BD), nitrogen compounds that cause bad odors, phosphorus compounds that cause environmental pollution, etc. have been demonstrated.
  • C OD chemical oxygen demand
  • BD biochemical oxygen demand
  • nitrogen compounds that cause bad odors, phosphorus compounds that cause environmental pollution, etc. have been demonstrated.
  • the SIID 2926-1b strain of the present invention can be said to be extremely excellent as a microorganism for wastewater treatment.
  • the septic tank is intended that wastewater per day 46m 3 processing the combined domestic wastewater and sewage from canteen, is obtained by employing the conventional activated sludge treatment method.
  • troubles such as inadequate purification of COD, BOD, etc. in the discharged water, which were presumed to be caused by setting the sludge load and flow rate load in the septic tank underestimated, occurred frequently.
  • the SIID 2926-1b stock according to the present invention was invested in the conventional septic tank.
  • COD, BOD and suspended solids in the wastewater were significantly reduced.
  • ammonia nitrogen which causes foul odors
  • nitrate nitrogen, nitrite nitrogen, and phosphorus compounds which cause environmental pollution
  • ammonia nitrogen and phosphorus compounds are 6% and 18.5%, respectively, of wastewater treated in conventional septic tanks. %, 2.0%, 3.7%.
  • the treatment with conventional septic tanks increased ammonia nitrogen and phosphorus compounds. It may be the result of decomposition by microorganisms other than SIID 2926-1b. From these results, it can be seen that the conventional septic tank cannot always reduce malodors and environmental pollutants. On the other hand, it was proved that the SIID 2926-1b strain of the present invention can significantly reduce malodors and environmental pollutants.
  • Example 1 In the culture broth prepared in Example 1 above, 400 parts by mass of rice bran, 100 parts by mass of rice bran, 100 parts by mass of coffee, 100 parts by mass of urea, 2 parts by mass of honey, sugar 2 parts by mass were added. Paying attention to the temperature rise due to the heat generated by the culture, the culture was allowed to stand for 5 days with occasional agitation so as not to exceed 55 ° C. Thereafter, the culture broth was air-dried, and the strain S I I D 2 9 2 6 -lb was put into a tentative state to obtain an environmental purification agent B. The water content of the environmental purification agent B was measured with a rice cereal moisture meter and found to be 13%. -"
  • Example 9 Disperse the environmental cleaner B obtained in Example 9 above into a 100-liter L-covered plastic bucket for approximately 30 to 10 o'clock in the morning for approximately 8 to 10 g every morning for 30 days. Then, every day, morning, noon, and evening meals were accumulated on top of it. The state of occurrence of odor when the lid was removed was judged by an agreement after three people checked at 7 am every morning.
  • Table 12 shows the state of odor generation before and after spraying of environmental cleaner B, where X is when there is odor, ⁇ when there is little odor, and ⁇ when there is no odor.
  • Table 1 2 shows the state of odor generation before and after spraying of environmental cleaner B, where X is when there is odor, ⁇ when there is little odor, and ⁇ when there is no odor.
  • Example 7 The environmental cleanser A of Example 7 above was sprayed on the ashtray of a private car approximately 20 to 7 mL every night for 20 days at 20 o'clock, and the occurrence of bad odor was checked by 3 people every morning at 7 o'clock. Judgment was made by agreement in Japan.
  • Table 13 shows the state of odor generation before and after spraying of environmental cleaner A, where X is when there is odor, ⁇ when there is little odor, and ⁇ when there is no odor.
  • Example 1 10 parts by mass of the culture solution prepared in Example 7 was added to 100 parts by mass of water and dispersed and mixed. This is an environmental cleaner. It was. The environmental purification agent C was sprayed to 3-5 mL per day over 60 days on bathroom walls and bathtubs that had been cleaned. After the experiment 60 days later, there was no power on the bathroom wall or bathtub. Therefore, the action of suppressing the occurrence of mold and slime of the environmental purifier according to the present invention was demonstrated.
  • Example 1 3 Suppression test for occurrence of force and slime
  • the environmental purification agent C of Example 12 above was added to the flush tank in the toilet every 3 to 3 ml over 60 days. After the experiment 60 days later, there was no power loss in the flush tank or the toilet bowl. Therefore, it was proved that the environmental purifier according to the present invention has an inhibitory action against mold and slime generation. '' Example 1 4 Slime suppression test
  • Example 1 5 Slime suppression test
  • Porous charcoal (average particle size: 2 to 5 mm) in 100 parts by mass of the culture solution prepared in Example 7 above was immersed in 100 parts by mass and stirred occasionally at 30 ° C. Let stand for days. Thereafter, only the porous charcoal was taken out and air-dried to obtain an environmental purification agent D having a moisture content of 12%. Put 1550 g of the environmental cleanser D in a polyester fiber non-woven bag, seal the mouth of the bag, and shape it according to the filter part of the bath for 4 hours. used. After three months, the state of occurrence of dust in a part of the filter was judged every day by an agreement after checking by three people at 7 am every morning, and no dust was found. Therefore, the slime-inhibiting action of the environmental cleaner according to the present invention was proved.
  • Activated carbon fibers (average fiber diameter: 100 ⁇ , average fiber length: 50 mm) were immersed in 100 parts by mass of the culture solution prepared in Example 7 above, and 3 2 The mixture was allowed to stand for 5 days with occasional stirring at ° C. Thereafter, only the activated carbon fiber was taken out and air-dried to obtain an environmental purification agent E having a moisture content of 14%.
  • the environmental purification agent E was put in a poly: ⁇ steal fiber non-woven bag, and the bag mouth was sealed, and it was used by being attached to a part of a filter for a bath for 24 hours in the same manner as in Example 17 above.
  • Example 9 Add about 30 g of the environmental cleanser B obtained in Example 9 above to a container in soil that accumulates pet dog's manure once a day for one month, and at the same time, supply an appropriate amount of water. Sprayed and stirred once every 3-5 days. Meanwhile, the container was used as usual and accumulated dog manure. For comparison, a similar test was conducted except that no environmental cleaner was used. Feces and urine were halved and accumulated in equal amounts in the examples and comparative examples.
  • Example 9 About 25 g of the environmental cleanser B obtained in Example 9 above is put together with raw garbage once a day for one month into a container in the soil where kitchen garbage is accumulated, and at the same time an appropriate amount of water is added. Sprayed and stirred once every 3-5 days. For comparison, a similar test was conducted separately except that no environmental cleaner was used. One day's worth of raw garbage was accumulated, halved, and equal amounts were accumulated in the examples and comparative examples.
  • the bacterium of the present invention has an excellent treatment capacity for harmful compounds. For example, it can exhibit extremely excellent effects such as reduction of organic compounds, nitrogen compounds and phosphorus compounds that cause eutrophication of rivers and seas, suppression of malodor, and suppression of causative substances of slime. Therefore, the present invention using the bacterium is extremely useful industrially as being capable of purifying the environment.

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  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
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  • Organic Chemistry (AREA)
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Abstract

L'invention concerne un procédé de purification environnementale dans lequel des composés organiques et inorganiques dangereux sont traités à l'aide de bactéries; un agent de purification environnementale à utiliser dans ce procédé; et une bactérie pouvant être utilisée dans ces procédé et agent de purification environnementaux. Ledit procédé est, essentiellement, caractérisé en ce qu'il consiste à mettre en contact une bactérie du genre Empedobacter et/ou sa culture avec un matériau à traiter, l'agent de purification environnemental étant caractérisé en ce qu'il contient une bactérie du genre Empedobacter et/ou sa culture.
PCT/JP2005/008336 2004-07-29 2005-04-25 Procede et agent de purification environnementaux WO2006011280A1 (fr)

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