WO2006011280A1

WO2006011280A1 - Environmental purification method and environmental purification agent

Info

Publication number: WO2006011280A1
Application number: PCT/JP2005/008336
Authority: WO
Inventors: Kiyoki Komori
Original assignee: Komori Bio Laboratory Inc.
Priority date: 2004-07-29
Filing date: 2005-04-25
Publication date: 2006-02-02
Also published as: WO2006011416A8; WO2006011416A1

Abstract

It is intended to provide an environmental purification method whereby harmful organic compounds and inorganic compounds are treated with a bacterium; an environmental purification agent to be used in this method; and a bacterium usable in these environmental purification method and agent. Namely, a method characterized by comprising contacting a bacterium belonging to the genus Empedobacter and/or its culture with a material to be treated; and an environmental purification agent characterized by containing a bacterium belonging to the genus Empedobacter and/or its culture.

Description

Description Environmental purification method and environmental purification agent Technical Field

The present invention relates to an environmental purification method, an environmental purification agent used in the method, and bacteria that can be used in these purification methods and environmental purification agents. Background art

In recent years, environmental impacts have become a problem as industrial technology develops and man-made increases. For example, nitrogen compounds contained in domestic wastewater and wastewater from food factories cause eutrophication in rivers and seas and cause red tides. In addition, manure and livestock sewage not only pollute rivers, but also cause odors. In addition, food waste from households has a high moisture content, so incineration requires not only a large amount of energy but also a foul odor.

Of the above environmental problems, physicochemical treatment and biological treatment are broadly used to treat domestic wastewater and wastewater from factories. Of these methods, physicochemical treatments such as agglomeration, precipitation, filtration and adsorption are excellent in processing speed. On the other hand, biological treatment is not efficient for wastewater containing a high concentration of pollutants, and it takes much time. Because of its superiority, biological treatment methods are often adopted.

However, bacteria that can degrade phosphorus compounds have not been found so far, and it has been thought that biological treatment cannot reduce phosphorus compounds in wastewater. In addition, in wastewater treatment using biological treatment methods, the search for bacteria that can treat pollutants efficiently is one of the major themes.

Odor generation is also a problem. In particular, it is necessary to quickly reduce amine-based compounds and sulfur-based compounds that cause bad odors in fetch-type toilets in areas without sewage facilities and toilets in trains that must be installed close to passenger seats. There is.

As a method of reducing malodor, there are a method of adsorbing the causative substance to a porous body such as activated carbon and a method of chemically decomposing it. However, since the adsorption capacity of the porous body is saturated, the removal is not possible. The odor capacity is limited. Also, chemical decomposition methods can be dangerous for home use. Therefore, various methods for reducing malodor using bacteria are being studied. For example, Japanese Patent Application Laid-Open No. 11-1137953 discloses a deodorizing apparatus including a carrier supporting microorganisms, a watering facility for spraying water on the carrier, and a water storage tank for collecting the water sprayed and having nitrifying bacteria. It is disclosed. However, the installation of watering and storage tanks is not only problematic in terms of equipment costs, but it is difficult to install the equipment in limited spaces such as in trains. In this regard, there are technologies that attempt to solve equipment problems by using bacteria that are particularly excellent in deodorizing action. Japanese Patent Laid-Open No. 5-153971 describes a malodor generation inhibitor using bacteria belonging to the species Bacillus me gaterium. However, the effects of the bacteria described in the examples of this document are qualitative, such as “the generation of malodors was suppressed”, and it is unclear how effective they are.

On the other hand, Japanese Patent Application Laid-Open No. 2001-70424 discloses a method for producing a biological deodorant carrying bacteria belonging to the genus Baci 11 1us such as Bacillusthuringiensis species. In the examples, specific treatments such as ammonia are disclosed. Data are also disclosed. However, as is clear from the attached diagram, the technology is to treat volatile compounds in the air, and to treat substances that directly cause odors. is not.

Further, in JP-A-6-190028 and JP-A-10-248556, there are a deodorant containing bacteria belonging to the genus C lostri du im and the genus Thermos and a deodorizer containing bacteria belonging to the genus Pseud omonas, respectively. An experimental example is disclosed in which methyl sulfide and methyl disulfide, which are malodorous substances, are treated. 'Examples of familiar environmental pollution include “numeric” that occurs in kitchen drains and drains, bathroom drains, bathtubs, toilet flush tanks, and water tanks. The true nature of this slime is a polysaccharide produced mainly by various germs, molds and algae. Therefore, a compound having a bactericidal action is used for suppressing slime. However, fungicides can be slow and can be dangerous to use at home.

Thus, Emp edobacter bacteria such as Emp edobacterbrevls are known to be used for bioconversion reactions such as adding hydroxyl groups to unsaturated fatty acids (Japanese Patent Laid-Open No. 15-144186). However, There is no example of using Emp edobacter bacteria for environmental purification such as wastewater treatment. Disclosure of the invention

As described above, wastewater and malodor treatment using bacteria has been conventionally performed. In addition, a technology for suppressing slime is also known. However, it is more efficient for biological treatment of harmful organic compounds and inorganic compounds, especially compounds that cause an increase in COD of wastewater, amine compounds and sulfur compounds that cause bad odor, and polysaccharides that cause slime. Technology is required. In particular, since bacteria that can treat phosphorus compounds that cause eutrophication in rivers and the like have not been found, bacteria capable of treating phosphorus compounds are eagerly desired.

Therefore, the problem to be solved by the present invention is to use an environmental purification method for treating harmful organic compounds and inorganic compounds by bacteria, an environmental purification agent used in the method, and these environmental purification method and environmental purification agent. It is to provide bacteria that can. In order to solve the above problems, the present inventors have searched for bacteria useful for environmental purification, and the Emp edobacter genus bacteria that have never been used for environmental purification so far have only COD, malodorous components, etc. of wastewater. In addition, the present invention was completed by finding that it has the ability to reduce the concentration of phosphorus compounds.

That is, the environmental purification method of the present invention is characterized in that the bacteria belonging to the genus Empedobacter and the culture thereof are brought into contact with the treatment object.

Further, the environmental purification agent of the present invention is characterized in that it contains bacteria belonging to the genus Empedobacter and / or a culture thereof.

Furthermore, the Emp e do b a c t er genus bacteria exhibiting particularly excellent effects with the environmental purification method and the environmental purification agent according to the present invention are S I I D 2926-1b strain (FERM A

P- 20108). Brief Description of Drawings

Fig. 1 Chromatographic results showing the fatty acid composition of S I ID 2926-1b strain.

Fig. 2 SIID 2926-1 is a molecular phylogenetic tree containing strain b and its related strains. BEST MODE FOR CARRYING OUT THE INVENTION

Among the bacteria belonging to the genus Empe dobacter, which are bacteria according to the present invention, the strain S 1 I D 2 926-1b is deposited at the Patent Microorganism Deposit Center (NPMD) under the deposit number: FER M AP-20108. SIID 2926-1b cell morphology, gram staining, spore presence, flagellar motility, and nu trientagar (Oxoid) observed with an optical microscope (Olympus, U-LH1000) Morphology of colonies cultured at 30 ° C for 24 hours, as well as B arrow, G. I. And Felth am, RKA, Cowandand Steel 's Man nu alforthe I dentiiication of Medical B acteri a. 3 rdedition Table 1 shows the test results of catalase reaction, oxidase reaction opi-glucose oxidation / fermentation (OZF) according to, C am bridge University Pres.

table 1

Table 2 shows the results of biochemical property tests using the API system (manufactured by BI OMER I EUX), which is a reagent for testing bacterial identification. Table 2

With regard to the above forms and properties, the strain SIID 2926-1b is similar to the fungal group classified in the F 1 avobacteriaceae family, but the genus species has not been identified. However, from the results of the examples described later, it can be concluded that the strain SIID 2926-1b is a bacterium classified into the genus Em pedobacter in the family F 1 a V obacteriaceae.

SII D2926-1b strain reduces COD, BOD, nitrogen compounds and phosphorus compounds in wastewater, suppresses the generation of amine compounds and sulfur compounds that cause foul odors, and suppresses polysaccharides that cause waste. Has an excellent action. Therefore, other bacteria belonging to the genus Empedobacter are also considered to have these effects and can be used for environmental purification in the same manner as the SIID2926-1b strain. Also, a plurality of bacteria belonging to the genus Empe dobacter may be used in combination.

The environmental purification method of the present invention is characterized by bringing the bacteria belonging to the genus Empedobacter and / or the culture thereof into contact with the treatment object. By the method of the present invention, harmful compounds in the environment, especially organic compounds that cause eutrophication of rivers, nitrogen compounds, phosphorus compounds; amine compounds and sulfur compounds that cause bad odors; The generation of molds can be suppressed.

The bacteria belonging to the genus Empedobacter used in the method of the present invention are as described above. Emp edobacter bacteria culture is Emp edobact It includes a medium used for culturing er genus bacteria and a compound, enzyme, etc. released into the medium with the growth of Emp edobacter genus bacteria. Empedobacter bacteria themselves can degrade harmful compounds, but enzymes produced by bacteria may have similar effects.

The medium is not particularly limited as long as it is suitable for cultivation of bacteria belonging to the genus Empedobacter. For example, water obtained by adding an appropriate amount of a carbon nutrient source such as sugar, a nitrogen nutrient source such as urea or peptone, or a trace element source such as yeast extract to water such as pure water, purified water, or distilled water can be used.

The object to be treated of the method of the present invention is not particularly limited as long as it contains a harmful compound or generates a harmful compound. Examples include wastewater that causes eutrophication of rivers and other wastewater such as domestic wastewater and food factory wastewater, odor sources such as manure and livestock wastewater, food waste such as raw garbage, and microbial sludge. Also, toilet rooms where bad odor is a problem, toilet bowl, dog and cat manure, ashtray, garbage container; wastewater, mold, bathroom drains, drains, walls, bathtubs, toilet tanks Emperedobacter bacteria and / or their cultures may be directly applied to water tanks, outdoor units of air conditioners, garbage containers, etc.

The environmental purification agent used in the method of the present invention is characterized in that it contains bacteria belonging to the genus Empedobacter and / or a culture thereof. The environmental cleansing agent of this effort makes harmful compounds in the environment, especially organic compounds that cause eutrophication of rivers, nitrogen compounds, phosphorus compounds; amine compounds and sulfur compounds that cause bad odors; It is possible to suppress polysaccharides that cause waste. Therefore, the environmental purification agent of the present invention is useful as a wastewater treatment agent, malodor inhibitor or deodorizer, slime inhibitor or polysaccharide generation inhibitor, mold generation inhibitor, and the like.

The environmental purification agent of the present invention may be one in which the Empedo bacter bacterium and / or its culture is directly administered to the treatment object, but may be in a form corresponding to the type of the treatment object.

For example, when the object to be treated is a toilet room or garbage itself, the bacteria belonging to the genus Empedobacter and / or its culture may be dispersed in a solvent and sprayed onto the object to be treated. Solvents that can be used in this case include water; alcohols such as ethanol; mixed solvents of water and alcohols. Such dispersion The concentration of the liquid is not particularly limited. For example, the solution may be about 5000 to 10000 times by mass added to Empedobacter bacteria. In addition, as long as the effects of Emp edobacter bacteria are not significantly reduced, those that can become a fungus bed and compositions thereof, pH adjusters, pigments, disinfectants, etc. may be used in combination. This dispersion shall also contain a culture solution of Empedobacter bacteria.

When the material to be treated is wastewater, the bacteria belonging to the genus Empedobacter and / or the culture thereof may be put into a conventional wastewater treatment facility such as a septic tank. In that case, it is not particularly necessary when the culture is introduced. However, when administering the bacterium itself, a nutrient source suitable for the growth of the bacterium, such as a carbon source, a nitrogen source, and a trace element source, is used. Add other conditions and make sure that the other conditions are met. Plant-based residues may be added as a nutrient source. This is because plant residues are not only excellent as a nutrient source for bacteria, but can also be reused for what should be discarded. Examples of such plant residues include rice bran, okara, sawdust, coffee lees, oil lees, soybean lees, pears, sugarcane bagasse, etc. For the bacteria of the present invention, rice lees and coffee lees Is the best.

A product obtained by culturing the bacterium of the present invention in the above-described plant residue is useful as it can be used for wastewater treatment as it is. The preparation method is, for example, adding the bacterium of the present invention and other nutrients to the plant residue, giving sufficient water, incubating at about 37-39 ° C, and fermenting for several days. This can be added to the workpiece as it is, or a dried product can be added to the workpiece.

In addition, the environmental purification agent of the present invention may be one in which an Empedobacter genus Opino or a culture thereof is supported on a carrier. Examples of the carrier that can be used include one or more selected from the group consisting of rice bran, coffee koji, soybean koji, coconut shell, activated carbon, porous silica, gauze, non-woven fabric, and fabric. This environmental purification agent can be produced by, for example, adding a culture solution of bacteria belonging to the genus Empe dobacter to a carrier and drying it appropriately.

The addition amount of the environmental purification agent bacterium according to the present invention may be appropriately adjusted according to, for example, the COD of wastewater, the degree of malodor, the presence of solid components such as garbage. When the material to be treated is wastewater, generally about 0.3 mg of bacterial cell weight is added to 1 L of wastewater. Add it. If the object to be treated is a toilet room or garbage, spread the environmental cleansing agent, which is a dispersion of Empedobacter bacteria and / or their cultures, to the extent that no foul odors occur. The strain SII D2926-1b grows at 30 ° C and 37 ° C as described above, so it is not necessary to control the temperature, and it can be treated at room temperature. May be incubated at an appropriate temperature. Specific treatment conditions may be determined by preliminary experiments. Hereinafter, the present invention will be described more specifically with reference to examples. However, the present invention is not limited by the following examples, and appropriate modifications are made within a range that can meet the purpose described above and below. These are all included in the technical scope of the present invention.

Example

Example 1 16SrDNA base sequence analysis

Genomic DNA was extracted from the cells of S I I D2926—lb strain (FERM AP—20108). For the extraction of genomic DNA, Insta GeneMatrix (manufactured by BIORAD) was used, and the protocol followed that of BIORAD. Using the extracted genomic DNA as a saddle type, a region of about 500 bp on the 5 ′ end side of 16 S rDNA was amplified by PCR. Thereafter, the amplified base sequence was sequenced to determine the base sequence. For PCR and cycle sequences, use the Micro S eq 500 16 S rDNA B acterial Identification PCR Kit (Applied Biosystems), and the Gene Recycler Gene Amp PCR System 9600 (Ap 1 ied B iosyst ems) and AB IPR I SM 3100 DNA Sequencer (App 1 ied B iosyst ems) were used as the DNA sequencer. In addition, the basic operation from PCR to cyclosequencing was in accordance with the protocol (P / N4346296 Rev. B) of Ap p lie e Bios stems.

Next, genus species were identified using the obtained base sequence information. First, a database of reference strains for each genus species, Micro S eq B acterial 500 0 Library v. 0023 (Ap plied B iosyst ems) The homology rate with known bacteria was determined using MicroSeq Microbial Identification System Vs. 1.4.1 as the analysis software and BLAST as the algorithm. Table 3 shows the results of the top 5 homology ratios.

Table 3

If the homology is 97% or less, they can be said to belong to different species (see: S tackebrandt E, Go etel BM (1 994), Taxon om icnote; alcefor DJ A—DNA reassociation and 1 6 S rRNA sequenceana 1 ysisintheresentsecies definitioninbacteriol ogy, Int. J. Sys. B acteriol., 44, pp 846-849). Therefore, S I I D 2926-1b strain was found to be a novel strain that does not belong to any known bacterial genus. As a precaution, we also performed identification using the international base sequence database (GeneBan kZDDB J / EMBL). Table 4 shows the results for the top 5 homology rates. Table 4

As a result, even when using the international nucleotide sequence database, it is the reference strain

The homology with Emp edobacterbrevis is 95%, and it can be said that the SIID 2926-1b strain does not belong to the Emp edobacterbrevis species. Some of the homology was high, but these were not the reference strains. In addition, even if the homology of the entire base sequence of 16 S r DNA is 100%, it does not necessarily prove that the strain is the same.

Although the above results suggest that the SIID 2926-1b strain is closely related to the genus Emp edobacter and the genus Flavobacterium, no homology was found to the reference strains classified into these genera. It was concluded that the bacterium was a completely new bacterium at the genus level, and was classified into the Flavobacteriaceae family that contains these genera. Example 2 Fatty acid composition analysis

S I I D 2926—1b strain was inoculated on a plate medium of T r i p t i c a c e S o y (S CD) A g a r (manufactured by B c t o n D i c k i in so) and cultured at 30 ° C. for 24 hours. The cells of 4 plates were scraped with a cell scraper and rubbed against the bottom of a 13 mL glass vial.

Add 1 mL of the incubation solution (sodium hydroxide 45 g, methanol 150 mL, distilled water 15 OmL) to this microbial cell, and leave the glass pail in a constant temperature state with a 100 ° C water pass. I put it. Five minutes after the start of the reaction, the mixture was taken out from the water path, stirred gently and returned, and the cells were further incubated for 30 minutes. Add 2 mL of methylation reagent (uniform mixture of 325 mL of 6.0 M hydrochloric acid and 275 mL of methanol) to the hatched sample solution and react for 10 minutes in a water bath set at 80 ° C. The fatty acid was methyl esterified.

Extract solution (mixed solution of 20 OmL of hexane and 200 mL of t-butyl methyl ether) 1. 25 mL was added to the sample solution, stirred for 10 minutes with a shaker, and allowed to stand. The aqueous layer separated into two layers was removed with a Pasteur pipette. To the remaining organic layer was added 3 mL of a washing solution (10.8 g of sodium hydroxide and 90 OmL of distilled water uniformly mixed) and stirred for 5 minutes with a shaker. As a result, a few drops of saturated saline were added. The two layers were separated. The upper 2 3 of the separated organic layer was taken out with a Pasteur pipette and transferred to a gas chromatography sample pail to obtain a measurement sample.

The fatty acid composition of the sample was measured using a gas chromatography system HP 6890 (manufactured by Hewlett-Packard). The results are shown in Figure 1. The In addition, a homology search with known bacterial species was performed on this result using the Sherlock Microdental Identification System. The results are shown in Table 5.

Table 5

In the above table, “Sum InFeature 3” is either C 16: 1 w7 c or C 15: 0 i so 2 OH, whose retention times are almost the same. In addition, the “similarity” is 1.000 when the bacterial species have the same composition, and if the value is 0.5 or more as a guide, it is similar to the fatty acid composition of the searched bacterial species. However, at 0.30 or less, it is considered that there are no bacterial species with similar fatty acid composition.

The results indicate that the SIID 2926-lb strain is closely related to Em pedobacterbrevis, which is classified in the Flavobacteriaceae family, but does not match the known bacterial species. . Example 3 Molecular Phylogenetic Tree

Regarding the reference strains included in the related strains of SIID 2926-1b strain estimated from the results of 16 Sr DN A base sequence analysis obtained in Example 1 above, the database (G en Bank / DDB J The upstream side of the 16 S rDN A nucleotide sequence was obtained from (EMBL) and a molecular phylogenetic tree was created. At that time, for alignment (processing to insert an appropriate gap into the sequence data so that the correspondence between the base sequences is the best), alignment software C lustal X (Thomson, JD et al., “T he C 1 ust 1 X wind ow sinterface: flexible strategy for multiple sequencealig nme ntaided by qualityana 1 ysistools ”, Nucleic Acids Re serach, 24, p p. 4876-4882 (1 997)). -Software MEG A 2 (Kumar, R. et al., “MEGA 2: Molecular Evo- lutionary Genetics Ana 1 ysissoft wa re”, Bioinfo rma tics, 1 7 pp. 1 2 44- 1 245 ( 2001)) was used. For the estimation of the molecular phylogenetic tree, the neighbor joining method (Saito, N. et al., “Theneighbor-joining me thod: an ew me tnodiorreconstructing phylogenetictrees”, Mol. Biol. Evolol, 4, p. -425 (1987)), a bootstrap showing the validity of the saddle shape (F e 1 senstein, J., “Confidencelimitsonphylogenies: anapproachusingteboot stra pJ, Ev olution, 39, p p. 783-791 ( 1 9 85)) was generated 1000 times, and the cluster containing SIID 2926-1b was supported by bootstrap values of 99% and 100%, as shown in Fig. 2. It can be seen that the 16 S r DNA base sequence of SIID 2926-1b strain is included in the cluster formed by the 16 S r DNA base sequence of Empedobacterbrevis. When considered together, it can be concluded that the strain SIID 2926-1b is a novel strain classified into the genus Emp edobacter. 4 deodorizing ability test

First, add SIID 2926-1 b strain, coffee koji, and urea to rice bran, incubate groundwater sprayed at 37-39 ° C for 5 days, and then air dry moderately to obtain SIID 2926-1 b A plant-based residue containing the strain was created. Next, 10 L of raw wastewater treatment plant water was taken into a 12 L sealed vacuum desiccator, and the plant residue was added at a rate of 10 g per 1 L of the raw water (total 100 g). The desiccator was sealed and incubated at 25 ° C with shaking. At predetermined time intervals, the air in the gas phase portion was extracted with a syringe, and the ammonia concentration (ν_ν ρ ρ m) in the gas phase portion was measured by the indophenol absorbance method based on the provisions of JIS K0099. The results are shown in Table 6. Table 6

From these results, the SIID 29 2 6-1b strain of the present invention can reduce the concentration of ammonia, which causes malodor from general raw manure water, to almost half in one hour. The ammonia concentration was maintained at a low level. Therefore, it was demonstrated that the S I I D 2 9 2 6-1b strain of the present invention has a high deodorizing ability. Example 5 Wastewater treatment test

First, the wastewater (discharge flow: 25 m ³ / day) from the food (kamaboko) manufacturing plant that is discharged after being treated with grease trap, pH, chemical oxygen demand, biochemical oxygen demand Amount, suspended solid content, total nitrogen content, total phosphorus content and n-hexane extractables (mineral oils and animal and plant fats and oils) were measured.

Next, a plant residue containing S I I D 29 2 6-lb strain used in Example 4 was added to the grease trap and stirred for 20 days. The pH of the wastewater after that was measured under the same conditions. The results are shown in Table 7.

Table 7

Units for numerical values other than pH are all mg / L.

As the results show, the SIID 2 9 2 6-1 b strain of the present invention has a chemical oxygen demand (C OD), biochemical oxygen demand (BD), nitrogen compounds that cause bad odors, phosphorus compounds that cause environmental pollution, etc. have been demonstrated. In particular, since it has been conventionally considered that biological treatment cannot reduce phosphorus compounds in wastewater, the SIID 2926-1b strain of the present invention can be said to be extremely excellent as a microorganism for wastewater treatment. Example 6 Wastewater treatment test

First, regarding the influent and effluent water of the septic tank (350 tanks) installed in the machine parts manufacturing factory, _PH , chemical oxygen demand, biochemical oxygen demand, suspended solids content, nitrogen compound content and The phosphorus compound content was measured by the method shown in Table 7. The septic tank is intended that wastewater per day 46m ³ processing the combined domestic wastewater and sewage from canteen, is obtained by employing the conventional activated sludge treatment method. However, troubles such as inadequate purification of COD, BOD, etc. in the discharged water, which were presumed to be caused by setting the sludge load and flow rate load in the septic tank underestimated, occurred frequently.

After the above measurement, 50 kg of plant residue containing S I I D2926 — lb strain used in Example 4 was added to the septic tank, and the same measurement was performed 4 days after the input. Furthermore, 4 kg of plant residue was added every week from the first input, for a total of 16 kg, and the same measurement was performed again after the final input. The results are shown in Table 8.

Table 8

According to the result, the SIID 2926-1b stock according to the present invention was invested in the conventional septic tank. As a result, COD, BOD and suspended solids in the wastewater were significantly reduced. In addition, ammonia nitrogen, which causes foul odors, and nitrate nitrogen, nitrite nitrogen, and phosphorus compounds, which cause environmental pollution, are 6% and 18.5%, respectively, of wastewater treated in conventional septic tanks. %, 2.0%, 3.7%. Of these compounds, the reason why ammonia nitrogen and phosphorus compounds increase once is not clear, but the treatment with conventional septic tanks increased ammonia nitrogen and phosphorus compounds. It may be the result of decomposition by microorganisms other than SIID 2926-1b. From these results, it can be seen that the conventional septic tank cannot always reduce malodors and environmental pollutants. On the other hand, it was proved that the SIID 2926-1b strain of the present invention can significantly reduce malodors and environmental pollutants. Example 7 Odor control test

S I I D 2926_lb strain 0.5 parts by mass, glucose 10 parts by mass, peptone 5 parts by mass opi yeast extract 2.5 parts by mass were added to 4000 parts by mass of water and cultured at 30 ° C. for 5 days. The culture solution (5 mL) was added to water (500 mL). This is environmental cleanser A. About 2 to 3 mL of the environmental cleanser A was applied to the sandbox, which is the cat's excrement site, for 10 days every morning at 8 o'clock in the morning, and the state of bad odor was determined by an agreement after checking by 3 people every morning at 7 o'clock. . Table 9 shows the occurrence of bad odor before and after spraying of environmental cleanser A, where X is when there is bad odor, △ when there is little bad odor, and ◯ when there is no bad odor.

Table 9

As shown in the results, although the environmental purification agent 剤 according to the present invention had a bad odor before use, the effect began to appear 1 day after the start of use, and the occurrence of bad odor was observed between 2 and 10 days. I couldn't. Therefore, the malodor control action of the environmental purification agent according to the present invention was demonstrated. Example 8 Odor control test

When the environmental cleanser A from Example 7 above is put into a tank of a draw-up toilet used by three family members for about 20 to about 15 to 2 O mL each morning for 20 days and the toilet lid is removed The state of occurrence of bad odors was judged by agreement after three people checked every morning at 7 am. Table 10 shows the state of odor generation before and after spraying of environmental cleanser A, where X is when there is odor, △ when there is little odor, and ◯ when there is no odor. Table 1 0

As shown in the results, although there was a bad odor until 1 day after the introduction of the environmental purification agent A according to the present invention, the effect began to appear on the 2nd day after the introduction, and the generation of a bad odor was observed between 4 and 20 days. There wasn't. Therefore, the malodor control action of the environmental purification agent according to the present invention was proved. Example 9 Odor control test

In the culture broth prepared in Example 1 above, 400 parts by mass of rice bran, 100 parts by mass of rice bran, 100 parts by mass of coffee, 100 parts by mass of urea, 2 parts by mass of honey, sugar 2 parts by mass were added. Paying attention to the temperature rise due to the heat generated by the culture, the culture was allowed to stand for 5 days with occasional agitation so as not to exceed 55 ° C. Thereafter, the culture broth was air-dried, and the strain S I I D 2 9 2 6 -lb was put into a tentative state to obtain an environmental purification agent B. The water content of the environmental purification agent B was measured with a rice cereal moisture meter and found to be 13%. -"

About 20 to 25 g of this environmental purification agent B was put into a toilet tank in the same manner as in Example 8 above, and the malodor control action was examined. The results are shown in Table 11.

Table 1 1

As shown in the results, although there was a bad odor until 1 day after the introduction of the environmental purification agent A according to the present invention, the effect began to appear on the 2nd day after the introduction, and the generation of the bad odor occurred from 5 to 20 days I was not able to admit. Therefore, the malodor control action of the environmental purification agent according to the present invention was proved. Example 1 0 Odor control test

Disperse the environmental cleaner B obtained in Example 9 above into a 100-liter L-covered plastic bucket for approximately 30 to 10 o'clock in the morning for approximately 8 to 10 g every morning for 30 days. Then, every day, morning, noon, and evening meals were accumulated on top of it. The state of occurrence of odor when the lid was removed was judged by an agreement after three people checked at 7 am every morning. Table 12 shows the state of odor generation before and after spraying of environmental cleaner B, where X is when there is odor, △ when there is little odor, and ◯ when there is no odor. Table 1 2

As shown in the results, although there was a bad odor until 1 day after the introduction of the environmental purification agent A according to the present invention, the effect began to appear on the 2nd day after the introduction, and the generation of a bad odor was recognized between 3 and 30 days. There wasn't. Therefore, the malodor control action of the environmental purification agent according to the present invention was proved. Example 1 1 Odor control test

The environmental cleanser A of Example 7 above was sprayed on the ashtray of a private car approximately 20 to 7 mL every night for 20 days at 20 o'clock, and the occurrence of bad odor was checked by 3 people every morning at 7 o'clock. Judgment was made by agreement in Japan. Table 13 shows the state of odor generation before and after spraying of environmental cleaner A, where X is when there is odor, △ when there is little odor, and ◯ when there is no odor.

Table 1 3

According to the result, it had a bad odor before the introduction of the environmental purification agent A according to the present invention. However, the effect began to appear on the first day after the injection, and no odor was observed between 20 and 20 days. Therefore, the malodor control action of the environmental purification agent according to the present invention was proved. Example 1 2 Test for suppressing the occurrence of force and slime

10 parts by mass of the culture solution prepared in Example 7 was added to 100 parts by mass of water and dispersed and mixed. This is an environmental cleaner. It was. The environmental purification agent C was sprayed to 3-5 mL per day over 60 days on bathroom walls and bathtubs that had been cleaned. After the experiment 60 days later, there was no power on the bathroom wall or bathtub. Therefore, the action of suppressing the occurrence of mold and slime of the environmental purifier according to the present invention was demonstrated. Example 1 3 Suppression test for occurrence of force and slime

The environmental purification agent C of Example 12 above was added to the flush tank in the toilet every 3 to 3 ml over 60 days. After the experiment 60 days later, there was no power loss in the flush tank or the toilet bowl. Therefore, it was proved that the environmental purifier according to the present invention has an inhibitory action against mold and slime generation. '' Example 1 4 Slime suppression test

Put 50 g of the environmental cleanser B obtained in Example 9 above into a polyester fiber non-woven bag, seal the bag mouth, and put it into the plastic plastic bottle at the kitchen drain used by three family members. It was. After 3 months, the occurrence of slime in the above plastic power bar was judged every day by an agreement after checking by three people, and no slime occurred. Therefore, the slime-inhibiting action of the environmental cleaner according to the present invention was proved. Example 1 5 Slime suppression test

Porous charcoal (average particle size: 2 to 5 mm) in 100 parts by mass of the culture solution prepared in Example 7 above was immersed in 100 parts by mass and stirred occasionally at 30 ° C. Let stand for days. Thereafter, only the porous charcoal was taken out and air-dried to obtain an environmental purification agent D having a moisture content of 12%. Put 1550 g of the environmental cleanser D in a polyester fiber non-woven bag, seal the mouth of the bag, and shape it according to the filter part of the bath for 4 hours. used. After three months, the state of occurrence of dust in a part of the filter was judged every day by an agreement after checking by three people at 7 am every morning, and no dust was found. Therefore, the slime-inhibiting action of the environmental cleaner according to the present invention was proved. Example 1 6 Suppression test

Activated carbon fibers (average fiber diameter: 100 μππι, average fiber length: 50 mm) were immersed in 100 parts by mass of the culture solution prepared in Example 7 above, and 3 2 The mixture was allowed to stand for 5 days with occasional stirring at ° C. Thereafter, only the activated carbon fiber was taken out and air-dried to obtain an environmental purification agent E having a moisture content of 14%. The environmental purification agent E was put in a poly: ^ steal fiber non-woven bag, and the bag mouth was sealed, and it was used by being attached to a part of a filter for a bath for 24 hours in the same manner as in Example 17 above. After two months, the occurrence of slime in the filter section was judged every day by an agreement after checking with three people every morning at 7 o'clock, and no slime occurred. Therefore, the slime-inhibiting action of the environmental cleaner according to the present invention was proved. Example 1 7 Organic compound decomposition test

Add about 30 g of the environmental cleanser B obtained in Example 9 above to a container in soil that accumulates pet dog's manure once a day for one month, and at the same time, supply an appropriate amount of water. Sprayed and stirred once every 3-5 days. Meanwhile, the container was used as usual and accumulated dog manure. For comparison, a similar test was conducted except that no environmental cleaner was used. Feces and urine were halved and accumulated in equal amounts in the examples and comparative examples.

One month after the start of the test, when the environmental purification agent B of the present invention was used, there was almost no increase in the accumulation of manure and very few odors. On the other hand, when environmental cleaner B was not used, feces and urine accumulated several times more, and foul odors were generated. Therefore, it was demonstrated that the environmental purification agent according to the present invention can decompose harmful organic compounds. Example 1 8 Organic compound decomposition test

About 25 g of the environmental cleanser B obtained in Example 9 above is put together with raw garbage once a day for one month into a container in the soil where kitchen garbage is accumulated, and at the same time an appropriate amount of water is added. Sprayed and stirred once every 3-5 days. For comparison, a similar test was conducted separately except that no environmental cleaner was used. One day's worth of raw garbage was accumulated, halved, and equal amounts were accumulated in the examples and comparative examples.

One month after the start of the test, when the environmental cleaner B of the present invention was used, there was almost no increase in the accumulation of garbage, and there was very little malodor generation. On the other hand, when the environmental cleanser B was not used, the accumulation of garbage was several times higher and a foul odor was also generated. Therefore, it was demonstrated that the environmental purification agent according to the present invention can decompose harmful organic compounds. Industrial applicability

The bacterium of the present invention has an excellent treatment capacity for harmful compounds. For example, it can exhibit extremely excellent effects such as reduction of organic compounds, nitrogen compounds and phosphorus compounds that cause eutrophication of rivers and seas, suppression of malodor, and suppression of causative substances of slime. Therefore, the present invention using the bacterium is extremely useful industrially as being capable of purifying the environment.

Claims

The scope of the claims

1. A method for environmental purification, which comprises bringing bacteria belonging to the genus Emp edobac ter and / or culture thereof into contact with an object to be treated.

2. The environmental purification method according to claim 1, wherein the strain S I I D2926—lb (FE RM AP—20108) is used as a bacterium belonging to the genus Emp e do abacter.

3. The environmental purification method according to claim 1 or 2, which reduces phosphorus compounds.

4. The environmental purification method according to any one of claims 1 to 3, which reduces a malodor-causing substance.

5. The environmental purification method according to any one of claims 1 to 4, which reduces slime.

6. An environmental purification agent comprising bacteria of the genus Emp edobac ter and / or cultures thereof.

7. The environmental purification agent according to claim 6, comprising S I D D 2926—1b strain (F ERM AP—20108) as a bacterium belonging to the genus Emp edobac ter.

8. The environmental purification agent according to claim 6 or 7, wherein the bacteria belonging to the genus Empedobacter and / or the culture thereof are dispersed in a solvent.

9. The environmental purification agent according to claim 6 or 7, wherein the Emp edobacter genus Opino or its culture is carried on a carrier.

10. The environment according to claim 9, wherein the carrier is one or more selected from the group consisting of rice bran, coffee lees, soybean lees, coconut husk, activated carbon, silica porous material, gauze, non-woven fabric, and fabric. Purifier.

11. S I I D 2926— 1b strain (FERM AP_20108).