WO2005102059A1

WO2005102059A1 - Control agent for disease caused by plant filamentous fungus, method of controlling disease caused by plant filamentous fungus and fertilizer therefor

Info

Publication number: WO2005102059A1
Application number: PCT/JP2005/007006
Authority: WO
Inventors: Hiroyuki Okamoto; Satoko Tuchiya; Takeshi Shirai
Original assignee: Asahi Breweries, Ltd.
Priority date: 2004-04-23
Filing date: 2005-04-11
Publication date: 2005-11-03
Also published as: TW200536483A

Abstract

A control agent for diseases caused by plant filamentous fungi, characterized in that a vegetable polyphenol having antimicrobial activity against plant pathogenic filamentous fungi is contained as an active ingredient. Conventional highly toxic crop dusting can be inhibited, and a highly safe control agent for diseases caused by plant filamentous fungi can be provided. Further, the problem of blight of vegetables by plant pathogenic filamentous fungi can be solved.

Description

Specification

Plant fungal disease controlling agent, method for controlling plant fungal disease, and fertilizer

Technical field

The present invention relates to a plant filamentous fungus controlling agent effective for phytopathogenic filamentous fungi such as Fusarium spp. Which is harmful in vegetable cultivation, a method for controlling plant filamentous fungal disease, and a fertilizer. is there.

Background art

[0002] It is known that the propagation of phytopathogenic filamentous fungi such as Fusarium spp. In open-field cultivation and nutrient solution cultivation of vegetables obliterates vegetables by blocking the conduit of the vegetables. Plant pathogenic microbes, especially Fusarium spp., Once they have begun to proliferate, take steps to control them.Pulsate the vegetables in which the phytopathogenic fungi have proliferated and suppress the spread of pathogenic fungi. The only reality is that there is no other way.

[0003] Therefore, highly toxic pesticides such as benlate wettable powder (trade name, manufactured by Sumitomo Chemical Co., Ltd.) are generally used when a disease is caused by pathogenic fungi or as a preventive measure.

[0004] Further, a microbial pesticide using a Pseudomonas genus bacterium exhibiting antibacterial activity against pathogenic fungi is known (Patent Document 1).

Patent Document 1: JP-A-11-187866.

Disclosure of the invention

Problems to be solved by the invention

[0005] In the conventional method for controlling pathogenic filamentous fungi using highly toxic pesticides, care must be taken not to inhale the pesticides when spraying the pesticides, and also in the vegetables after spraying. Needed to measure pesticide residues and confirm its safety. Therefore, in order to reduce residual pesticides, the time of application of pesticides was restricted, and it was not possible to apply toxic strong V and pesticides immediately before shipping.

[0006] Therefore, when spraying highly toxic pesticides, meticulous attention is required, the work is complicated, and there are problems associated with health risks.

[0007] The microbial pesticide of Patent Document 1 is highly safe, but is a strain of Pseudomonas sp. Must be cultured, which is not suitable for mass production.

[0008] The problem to be solved by the present invention is to suppress the conventional highly toxic pesticide spraying, to provide a highly safe plant filamentous fungus controlling agent, and to question the death of vegetables caused by phytopathogenic filamentous fungi. It is to solve the problem.

Means for solving the problem

[0009] The present inventors diligently searched for a highly safe, naturally occurring compound that exhibits antibacterial activity against pathogenic filamentous fungi. As a result, they found that plant polyphenols were effective and completed the present invention. To lj.

[0010] That is, the present invention for solving the above-mentioned problems has the following contents.

(1) A plant filamentous fungus controlling agent comprising, as an active ingredient, a plant polyphenol having antibacterial properties against phytopathogenic fungi.

(2) The plant filamentous fungus controlling agent according to (1), wherein the plant polyphenol is derived from apple.

(3) The plant filamentous fungus controlling agent according to (1) or (4), wherein the phytopathogenic filamentous fungus is a filamentous fungus belonging to the genus Fusarium. (4) Control of plant fungal disease characterized by treating soil and Z or plants with a plant fungal disease controlling agent containing a plant polyphenol having an antibacterial activity against phytopathogenic fungi as an active ingredient. Method.

(5) The method for controlling a fungal disease of a plant according to item 4, wherein the method is derived from the plant polyphenolic alcohol.

(6) The method for controlling a plant filamentous fungal disease according to (4) or (5), wherein the phytopathogenic filamentous fungus is a filamentous fungus belonging to the genus Fusarium.

(7) A fertilizer having a plant filamentous fungal control effect, comprising a plant polyphenol having an antibacterial property against phytopathogenic fungi and a fertilizer as active ingredients.

(8) The fertilizer according to claim 7, wherein the plant polyphenol is derived from an apple.

(9) The fertilizer having a plant filamentous fungal disease controlling effect according to (7) or (8), wherein the phytopathogenic filamentous fungus is a filamentous fungus belonging to the genus Fusarium. The invention's effect

[0011] Extracts mainly composed of plant polyphenols extracted from plants are highly safe and naturally derived compounds, many of which have been recognized as food additives. Therefore, compared with the case where a conventional highly toxic pesticide is used, it is possible to control the fungal disease efficiently and very safely. In addition, plant polyphenols such as apple polyphenols are highly safe and naturally occurring compounds, and therefore, eating vegetables containing plant polyphenols does not adversely affect the human body.

[0012] Further, the fertilizer of the present invention has an effect of controlling a fungal disease of a plant, and thus can efficiently control a fungal disease of a plant simultaneously with fertilization.

BEST MODE FOR CARRYING OUT THE INVENTION

[0013] The plant polyphenol used in the present invention is derived from plants such as apples, grapes, tea, herbs, and spices.

[0014] In particular, apple polyphenol is a general term for polyvalent aromatic hydroxy conjugates contained in apple fruit, and among the apple polyphenols, there are proanthocyanins, catechins, chlorogenic acid, caffeine, and the like. It contains phenolic carboxylic acids such as acids and p-tamaric acid, and flavonoids such as quercetin glycosides and chalcone glycosides.

[0015] The method for preparing apple polyphenol is not particularly limited, but fruit juice containing fruit polyphenol can be easily obtained by pressing or centrifuging. By treating the squeezed juice and extract with an adsorbent, the polyphenol fraction can be purified. That is, apple fruits are clarified by squeezing or centrifuging, passed through a synthetic styrene-dibutylbenzene-based synthetic adsorption resin to adsorb the polyphenol component, and washed with water to completely remove sugars and organic acids. After that, the apple polyphenol fraction can be purified by elution with aqueous ethanol.

[0016] By concentrating the purified polyphenol fraction, a concentrated solution in which apple polyphenol is concentrated can be obtained. Further, by spray-drying or freeze-drying this concentrated liquid, apple polyphenol on powder can be obtained.

[0017] Commercially available products may be used as the plant polyphenol (apple, grape seed, tea, mint, evening primrose, each extract, etc.). [0018] The apple as a raw material of the apple polyphenol can be used regardless of the variety. Further, the apple as a raw material may be a pruned immature apple, a non-grade apple, or an apple that has been damaged by falling or the like. Since immature apple fruit contains about 50 times as much apple polyphenol as mature apple fruit, it is preferable to use immature fruit as a raw material.

[0019] Examples of pathogenic plant filamentous fungi to be used as the plant filamentous fungus controlling agent of the present invention include, for example, Fusarium solani f. Sp phaseoli, Fusarium anguioides, and Fusarium.

avenaceum, Fusarium bulbigenum, Fusarium caucasicum, Fusarium cnlamydosporum, Fusarium

coccophilum, Fusarium crookwellense, Fusarium culmorum, Fusarium

decemcellulare, Fusarium

equiseti, Fusarium graminearum, Fusarium lateritium, Fusarium lini, Fusarium merismoides, Fusarium moniliforme, Fusarium nivale, Fusarium oxysporum, Fusarium

pallidoroseum, Fusarium roseum, Fusarium semitectum, Fusarium solani, Fusarium splendens, Fusarium vasinfectum, Fusarium acuminatum Fusarium anthophilum, Fusarium

aquaeductuum, Fusarium arthrosporioides, Fusarium beomiforme, Fusarium buharicum, Fusarium

camptoceras, Fusarium chlamydosporum Fusarium ciliatum, Fusarium coccidicola, Fusarium

coeruleum, Fusarium compactum, Fusarium concolor, Fusarium dimerum, Fusarium dlamini ゝ Fusarium episphaeria, Fusarium episphaeropsis, Fusarium epistromum, Fusarium

flocciferum, Fusarium fusarioides, Fusarium heterosporum, Fusarium inflexum, Fusarium

javanicum, Fusarium lunulosporum, Fusarium melanochlorum, Fusarium napiforme, Fusarium nygamai, Fusarium orthoceras, Fusarium poae, Fusarium polyphialidicum, Fusarium proliferatum, Fusarium redolens, Fusarium rigidiuscula, Fusarium saccharic Fusarium

sambucinum, Fusarium scirpi ゝ Fusarium semitectum, Fusarium sporotrichiella, Fusarium

sporotrichioides, Fusarium stilboides, Fusarium subglutinans, Fusarium sulphureum, Fusarium

tabacinum, Fusarium trichothecioides, Fusarium tricinctum, Fusarium ventricosum, Fusarium

Verticulioides, Fusarium xylarioides, and Fusarium zonatum are capable of storming S.

[0020] Since the plant polyphenol extract is water-soluble, it can be diluted with water to control pathogenic fungi. That is, an aqueous solution of plant polyphenol should be sprayed or applied to vegetables in which pathogenic fungi have propagated, or sprayed on unvegetated vegetables in a preventive manner. In addition, the roots of vegetable seedlings can be immersed in an aqueous solution of plant polyphenol to control plant fungal disease. By immersing vegetable seeds and vegetable seeds in an aqueous solution of plant polyphenol, plant filamentous fungi can be controlled. Furthermore, by spraying or irrigating an aqueous solution of plant polyphenol on a seedling raising soil or a cultivation field, it is possible to prevent plant fungal diseases in a preventive manner.

[0021] It is also possible to add and mix a powdery plant polyphenol to the seedling raising soil to control plant pathogenic fungi in the soil.

[0022] It is more preferable to combine a plant polyphenol and a fertilizer, since the fungal disease can be efficiently controlled simultaneously with the fertilization. Fertilizers that can be combined with plant polyphenols include, for example, chemical fertilizers such as Kumiai Kasei Fertilizer (Kumiai Kasei Co., Ltd.) or organic fertilizers such as Organic Combined Fertilizer (Sakata Seed Co., Ltd.). Or liquid fertilizers such as "Hyponex Vegetable Liquid" (manufactured by Hyponex Japan) and foliar spraying agents such as "Calplus" (manufactured by Otsuka Chemical Co., Ltd.).

The

[0023] In addition, in the nutrient solution cultivation of vegetables and flowers, the nutrient solution and medium used are plant polyphenols. By adding and using this, it is possible to effectively control plant fungal disease in hydroponics.

[0024] Even if the plant fungal disease controlling agent of the present invention is sprayed on soil, useful microorganisms and insects in the soil are not killed, so that no burden is imposed on the environment.

[0025] Furthermore, since plant polyphenols are almost odorless and safe, spray them near houses.

[0026] The plant polyphenol, which is an active ingredient of the plant fungal disease controlling agent of the present invention, is a naturally derived compound recognized as a food additive. Therefore, when spraying, rubber gloves are used for conventional agricultural chemicals. No need to wear and handle or work with gas mask

[0027] Even if the plant filamentous fungus controlling agent of the present invention remains in vegetables, it is harmless, so that the spraying time of the plant filamentous fungus controlling agent of the present invention is not restricted, and the plant fungicide is sprayed on vegetables immediately before shipment. You can do it.

When spraying as an aqueous solution, the concentration of the plant polyphenol is not particularly limited, and it may be appropriately diluted according to the purpose of use.

[0029] To the extent that the effects of the present invention are not impaired, a spreading agent such as "Approach" (manufactured by Maruwa Noo Chemical Co., Ltd.) or an auxiliary agent such as a yeast-derived rooting promoter may be added within a range. Good.

[0030] The mechanism of the present invention for controlling plant filamentous fungal diseases is unknown, but it is considered that plant polyphenols inhibit the mycelial growth of filamentous fungi, inhibit the formation of sclerotium, or inhibit germination.

Hereinafter, the present invention will be described in detail with reference to examples, but the present invention is not limited to the examples.

Example 1

[0032] Antibacterial activity against phytopathogenic fungi was measured using apple polyphenol (apple extract: trade name "Applephenone", powder form, manufactured by Asahi Breweries, Ltd.). Antibacterial activity of apple polyphenol was measured using Fusarium solani f. Sp phaseoli as a phytopathogenic fungus.

First, apple polyphenol was dissolved in sterile water to prepare a 4 wt% apple polyphenol aqueous solution. Using a 96-well microplate, add 4 wt% aqueous solution of apple polyphenol to each well The solution was added and sequentially diluted with sterile water. 10 ⁴ Zml to be green root rot fungus (Fusanum

spores of S. solani f. sp phaseoli) were added to a twice-concentrated PD medium and cultured at 30 ° C. After 24 hours and 48 hours, the presence or absence of spore germination was observed under a microscope to evaluate the antibacterial activity. The results are shown in Table 1. For comparison, a system without addition of an antibacterial agent was used as a control, and a common filamentous fungus, Aspergillus

oryzae AOK12-2) was used as a control to test the antibacterial activity. Table 1 shows the results of the antibacterial test.

[0034] [Table 1]

[0035] Evaluation criteria + +: Strong antibacterial activity (no spore germination)

+: Antibacterial activity (very few spore germination)

: No antibacterial activity (spore germination)

[0036] As is clear from the results shown in Table 1, the system in which 0.5% by weight of apple polyphenol was added was obtained from Fusarium.

solani f. sp phaseoli can suppress spore germination, and apple polyphenols can control plant fungal diseases with high antibacterial activity against pathogenic fungi of Fusarium .

Industrial applicability

[0037] Vegetable polyphenols such as apple polyphenol, which are active ingredients of the plant filamentous fungus controlling agent of the present invention, are highly safe naturally occurring compounds that have been recognized as food additives. Therefore, compared with the conventional case of using a highly toxic pesticide, it is possible to control plant filamentous fungi efficiently and very safely. Also, since plant polyphenols are highly safe and naturally occurring compounds, eating vegetables containing plant polyphenols does not adversely affect the human body.

Claims

The scope of the claims

[1] A plant filamentous fungus controlling agent comprising, as an active ingredient, a plant polyphenol having antibacterial activity against phytopathogenic filamentous fungi.

[2] The plant filamentous fungus controlling agent according to [1], which is derived from the plant polyphenolic alcohol.

[3] The plant filamentous fungus controlling agent according to [1] or [2], wherein the phytopathogenic filamentous fungus is a filamentous fungus belonging to the genus Fusarium.

[4] Control of plant fungal disease characterized by treating soil and Z or a plant with a plant fungal disease controlling agent containing a plant polyphenol having an antibacterial activity against phytopathogenic fungi as an active ingredient Method.

5. The method for controlling a fungal disease of a plant according to claim 4, wherein the method is derived from the plant polyphenolic alcohol.

6. The method according to claim 4, wherein the phytopathogenic filamentous fungus is a filamentous fungus belonging to the genus Fusarium.

[7] A fertilizer having a plant filamentous fungal control effect, comprising a plant polyphenol and a fertilizer having antibacterial properties against phytopathogenic fungi as active ingredients.

[8] The fertilizer according to claim 7, wherein the fertilizer is derived from the plant polyphenolic alcohol.

9. The fertilizer according to claim 7, wherein the phytopathogenic filamentous fungus is a filamentous fungus belonging to the genus Fusarium.