WO2005102041B1 - Crustacean expression vector - Google Patents
Crustacean expression vectorInfo
- Publication number
- WO2005102041B1 WO2005102041B1 PCT/US2005/012979 US2005012979W WO2005102041B1 WO 2005102041 B1 WO2005102041 B1 WO 2005102041B1 US 2005012979 W US2005012979 W US 2005012979W WO 2005102041 B1 WO2005102041 B1 WO 2005102041B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- construct
- promoter
- ires
- protein
- cell
- Prior art date
Links
- 241000238424 Crustacea Species 0.000 title claims abstract 10
- 239000013604 expression vector Substances 0.000 title 1
- 108020004684 Internal Ribosome Entry Sites Proteins 0.000 claims abstract 24
- 102000004169 proteins and genes Human genes 0.000 claims abstract 24
- 238000000034 method Methods 0.000 claims abstract 19
- 241000238557 Decapoda Species 0.000 claims abstract 13
- 108700026244 Open Reading Frames Proteins 0.000 claims abstract 12
- 241000709664 Picornaviridae Species 0.000 claims abstract 7
- 206010033799 Paralysis Diseases 0.000 claims abstract 5
- 241000700605 Viruses Species 0.000 claims abstract 5
- 108020004707 nucleic acids Proteins 0.000 claims abstract 5
- 102000039446 nucleic acids Human genes 0.000 claims abstract 5
- 150000007523 nucleic acids Chemical class 0.000 claims abstract 5
- 241000238631 Hexapoda Species 0.000 claims abstract 3
- 108090000623 proteins and genes Proteins 0.000 claims 24
- 210000004027 cell Anatomy 0.000 claims 15
- 241000439574 Decapod penstyldensovirus 1 Species 0.000 claims 4
- 241001465754 Metazoa Species 0.000 claims 3
- 241001265687 Taura syndrome virus Species 0.000 claims 3
- 241000696962 White spot syndrome virus Species 0.000 claims 3
- 108091026898 Leader sequence (mRNA) Proteins 0.000 claims 2
- 241000927735 Penaeus Species 0.000 claims 2
- 210000004102 animal cell Anatomy 0.000 claims 2
- 230000001681 protective effect Effects 0.000 claims 2
- 230000001225 therapeutic effect Effects 0.000 claims 2
- 230000003612 virological effect Effects 0.000 claims 2
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 claims 1
- 108091029795 Intergenic region Proteins 0.000 claims 1
- 241000272443 Penelope Species 0.000 claims 1
- 238000004520 electroporation Methods 0.000 claims 1
- 239000003550 marker Substances 0.000 claims 1
- 239000013612 plasmid Substances 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 230000010076 replication Effects 0.000 claims 1
- 108010001267 Protein Subunits Proteins 0.000 abstract 2
- 241000251468 Actinopterygii Species 0.000 abstract 1
- 108700028146 Genetic Enhancer Elements Proteins 0.000 abstract 1
- 241000237852 Mollusca Species 0.000 abstract 1
- 102000002067 Protein Subunits Human genes 0.000 abstract 1
- 238000010353 genetic engineering Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 235000015170 shellfish Nutrition 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
- A01K67/0337—Genetically modified Arthropods
- A01K67/0338—Genetically modified Crustaceans
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/70—Invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/02—Animal zootechnically ameliorated
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/03—Animal model, e.g. for test or diseases
- A01K2267/0393—Animal model comprising a reporter system for screening tests
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/90—Vectors containing a transposable element
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/60—Vector systems having a special element relevant for transcription from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Environmental Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Methods and constructs for genetic manipulation of one or more of shrimp, shellfish, mollusks, and fish are disclosed. The nucleic acid construct includes a promoter and an internal ribosome entry site of an insect picornavirus, such as a cricket paralysis-like picornavirus. One or more open reading frames can be operably associated with one or both of the promoter and the internal ribosome entry site, and one or more proteins or protein subunits can be expressed upon introduction of the construct into a host cell, such as into a shrimp. Method for producing immortalized crustacean cell lines using enhancer elements derived from shrimp and/or shrimp viruses are also described.
Claims
1. A non-naturally-occurring nucleic acid construct for expressing a cistron in a crustacean cell, the construct comprising a virus promoter and an insect picomavirus (IPV) internal ribosome entry site (IRES), wherein the promoter and the IRES are selected such that when the cistron is operably linked with the promoter and the IRES, the cistron is capable of expression in the cell.
2. The construct of claim 1 , wherein the promoter and the HRES are derived from the same EPV.
3. The construct of claim 1, wherein the promoter is a promoter of a cricket paralysis-like picornavirus.
4. The construct of claim 1, wherein the IRES is a HRES of a cricket paralysis-like picornavirus.
5. The construct of claim 1, wherein the IRES is the IRES of the 5 '-untranslated region of a cricket paralysis-like picornavirus.
6. The construct of claim 1, wherein the IRES is the IRES of the intergenic region of a cricket paralysis-like picornavirus having at least two ORFs.
7. The construct of claim 1, wherein the promoter is an infectious hypodermal and hematopoietic necrosis virus (IHHNV) promoter.
8. The construct of claim 1, wherein the IRES is a Taura syndrome virus (TSV) IRES.
9. The construct of claim 8, wherein the ERES is the IRES of the 5'-untranslated region of TSV.
10. The construct of claim 8, wherein the promoter is an IHHNV promoter.
11. The construct of claim 10, wherein the promoter is at least one of the P2 and P61 promoters of IHHNV. 38
12. The construct of claim 8, wherein the promoter is a white spot syndrome virus (WSSV) promoter.
13. The construct of claim 1, wherein the promoter is a promoter of an IPV that infects shrimp.
14. The construct of claim 1, wherein the IRES is an IRES of an IPV that infects shrimp.
15. The construct of claim 14, wherein the promoter is a promoter of an EPV that infects shrimp.
16. The construct of claim 1, wherein the construct further comprises an animal cell origin of replication site (ORS).
17. The construct of claim 16, wherein the animal is a crustacean.
18. The construct of claim 17, wherein the crustacean is a shrimp.
19. The construct of claim 18, wherein the shrimp is a.Penaeus shrimp.
20. The construct of claim 1, further comprising a first open reading frame (ORF) operably associated with the promoter.
21. The construct of claim 20, wherein the ORF is not interrupted by an intron.
22. The construct of claim 20, wherein the ORF encodes a detectable marker.
23. The construct of claim 1, further comprising a second ORF operably associated with the IRES.
24. The construct of claim 23, further comprising a first ORF operably associated with the promoter.
25. The construct of claim 24, wherein at least one of the first and second ORFs encodes a protein not normally expressed in a shrimp.
26. The construct of claim 1, further comprising a transposable element (TE).
27. The construct of claim 26, wherein the TE is homologous to the Drosophila Penelope TE sequence.
28. The construct of claim 26, wherein the TE is a TE of WSSV.
29. The construct of claim 26, wherein the TE is a TE of an IPV.
30. A method of expressing a protein in a crustacean cell, the method comprising introducing into the cell a non-naturally-occurring nucleic acid construct, the construct comprising a viral promoter and an IPV ERES, wherein an ORF encoding the protein is operably linked with at least one of the promoter and the IRES.
31. The method of claim 30, wherein the protein is a eukaryotic protein.
32. The method of claim 30, wherein the protein is a shrimp protein.
33. The method of claim 30, wherein the crustacean is a shrimp.
34. The method of claim 33, wherein the shrimp is a Penaeus shrimp.
35. The method of claim 30, wherein the construct is a plasmid.
36. The method of claim 30, wherein the construct is introduced into the cell using a virus vector.
37. The method of claim 30, wherein the construct is introduced into the cell ballistically.
38. The method of claim 30, wherein the construct is introduced into the cell by electroporation.
39. The method of claim 30, wherein the construct further comprises a TE.
40. The method of claim 30, wherein the protein is a protein that exerts a protective effect on the cell.
41. The method of claim 30, wherein the protein is a protein that exerts a protective effect on an animal that comprises the cell.
42. The method of claim 30, wherein the protein is a protein that exerts a therapeutic effect on the cell.
43. The method of claim 30, wherein the protein is a protein that exerts a therapeutic effect on an animal that comprises the cell.
44. The method of claim 30, wherein the protein is a protein that exerts a regulatory effect on expression of an endogenous gene of the cell.
45. A method of expressing a protein in a marine animal cell, the method comprising introducing into the cell a non-naturally-occurring nucleic acid construct, the construct comprising a viral promoter and an IPV IRES, wherein an ORF encoding the protein is operably linked with at least one of the promoter and the IRES and wherein the promoter and the IRES are selected such that the ORF is capable of expression in a crustacean cell.
46. A non-naturally-occurring nucleic acid construct for expressing a cistron in a crustacean cell, the construct comprising a virus promoter and an insect picornavirus (IPV) internal ribosome entry site (IRES), wherein at least one of the promoter and the IRES is from a virus known to infect the crustacean.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP05740192A EP1756276A4 (en) | 2004-04-15 | 2005-04-15 | Crustacean expression vector |
| US11/587,770 US7973148B2 (en) | 2004-04-15 | 2005-04-15 | Crustacean expression vector |
| US13/176,026 US20110269225A1 (en) | 2004-04-15 | 2011-07-05 | Crustacean expression vector |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US56245904P | 2004-04-15 | 2004-04-15 | |
| US60/562,459 | 2004-04-15 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US13/176,026 Continuation US20110269225A1 (en) | 2004-04-15 | 2011-07-05 | Crustacean expression vector |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| WO2005102041A2 WO2005102041A2 (en) | 2005-11-03 |
| WO2005102041A3 WO2005102041A3 (en) | 2006-03-30 |
| WO2005102041B1 true WO2005102041B1 (en) | 2006-11-09 |
Family
ID=35197461
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2005/012979 WO2005102041A2 (en) | 2004-04-15 | 2005-04-15 | Crustacean expression vector |
Country Status (2)
| Country | Link |
|---|---|
| EP (1) | EP1756276A4 (en) |
| WO (1) | WO2005102041A2 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2912665C (en) | 2013-05-15 | 2021-03-02 | Robert Kruse | Intracellular translation of circular rna |
| US20210277393A1 (en) * | 2018-07-24 | 2021-09-09 | Mayo Foundation For Medical Education And Research | Circularized engineered rna and methods |
| KR20210116551A (en) * | 2019-01-15 | 2021-09-27 | 시오크 미트 피티이. 엘티디. | Isolation and Culture of Muscle and Adipocytes from Crustaceans |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0019745D0 (en) * | 2000-08-10 | 2000-09-27 | Univ Surrey | Gene expression element |
| JP2002306168A (en) * | 2001-01-25 | 2002-10-22 | National Institute Of Agrobiological Sciences | New translation activity-promoting higher structure |
| WO2003048325A2 (en) * | 2001-12-04 | 2003-06-12 | University Of Hawaii | Nucleotide sequences of shrimp beta-actin and actin promoters and their use in genetic transformation technology |
-
2005
- 2005-04-15 EP EP05740192A patent/EP1756276A4/en not_active Withdrawn
- 2005-04-15 WO PCT/US2005/012979 patent/WO2005102041A2/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| WO2005102041A2 (en) | 2005-11-03 |
| WO2005102041A3 (en) | 2006-03-30 |
| EP1756276A4 (en) | 2008-10-29 |
| EP1756276A2 (en) | 2007-02-28 |
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