WO2005102041A3 - Crustacean expression vector - Google Patents

Crustacean expression vector Download PDF

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Publication number
WO2005102041A3
WO2005102041A3 PCT/US2005/012979 US2005012979W WO2005102041A3 WO 2005102041 A3 WO2005102041 A3 WO 2005102041A3 US 2005012979 W US2005012979 W US 2005012979W WO 2005102041 A3 WO2005102041 A3 WO 2005102041A3
Authority
WO
WIPO (PCT)
Prior art keywords
shrimp
crustacean
promoter
picornavirus
entry site
Prior art date
Application number
PCT/US2005/012979
Other languages
French (fr)
Other versions
WO2005102041B1 (en
WO2005102041A2 (en
Inventor
Arun K Dhar
Thomas F C Allnutt
Original Assignee
Advanced Bionutrition Corp
Arun K Dhar
Thomas F C Allnutt
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Bionutrition Corp, Arun K Dhar, Thomas F C Allnutt filed Critical Advanced Bionutrition Corp
Priority to US11/587,770 priority Critical patent/US7973148B2/en
Priority to EP05740192A priority patent/EP1756276A4/en
Publication of WO2005102041A2 publication Critical patent/WO2005102041A2/en
Publication of WO2005102041A3 publication Critical patent/WO2005102041A3/en
Publication of WO2005102041B1 publication Critical patent/WO2005102041B1/en
Priority to US13/176,026 priority patent/US20110269225A1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
    • A01K67/0337—Genetically modified Arthropods
    • A01K67/0338—Genetically modified Crustaceans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/70—Invertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Methods and constructs for genetic manipulation of one or more of shrimp, shellfish, mollusks, and fish are disclosed. The nucleic acid construct includes a promoter and an internal ribosome entry site of an insect picornavirus, such as a cricket paralysis-like picornavirus. One or more open reading frames can be operably associated with one or both of the promoter and the internal ribosome entry site, and one or more proteins or protein subunits can be expressed upon introduction of the construct into a host cell, such as into a shrimp. Method for producing immortalized crustacean cell lines using enhancer elements derived from shrimp and/or shrimp viruses are also described.
PCT/US2005/012979 2004-04-15 2005-04-15 Crustacean expression vector WO2005102041A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US11/587,770 US7973148B2 (en) 2004-04-15 2005-04-15 Crustacean expression vector
EP05740192A EP1756276A4 (en) 2004-04-15 2005-04-15 Crustacean expression vector
US13/176,026 US20110269225A1 (en) 2004-04-15 2011-07-05 Crustacean expression vector

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US56245904P 2004-04-15 2004-04-15
US60/562,459 2004-04-15

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/176,026 Continuation US20110269225A1 (en) 2004-04-15 2011-07-05 Crustacean expression vector

Publications (3)

Publication Number Publication Date
WO2005102041A2 WO2005102041A2 (en) 2005-11-03
WO2005102041A3 true WO2005102041A3 (en) 2006-03-30
WO2005102041B1 WO2005102041B1 (en) 2006-11-09

Family

ID=35197461

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/012979 WO2005102041A2 (en) 2004-04-15 2005-04-15 Crustacean expression vector

Country Status (2)

Country Link
EP (1) EP1756276A4 (en)
WO (1) WO2005102041A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2996697B1 (en) 2013-05-15 2019-06-26 Robert Kruse Intracellular translation of circular rna
EP3826643A4 (en) * 2018-07-24 2022-05-18 Mayo Foundation for Medical Education and Research Circularized engineered rna and methods
KR102687238B1 (en) * 2019-01-15 2024-07-23 시오크 미트 피티이. 엘티디. Isolation and culture of muscle and fat cells from crustaceans

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0019745D0 (en) * 2000-08-10 2000-09-27 Univ Surrey Gene expression element
JP2002306168A (en) * 2001-01-25 2002-10-22 National Institute Of Agrobiological Sciences New translation activity-promoting higher structure
AU2002365601A1 (en) * 2001-12-04 2003-06-17 University Of Hawaii Nucleotide sequences of shrimp beta-actin and actin promoters and their use in genetic transformation technology

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
MARTINEZ-SALAS E. ET AL: "Picornavirus IRES: Structure function relationship", CURRENT PHARMACEUTICAL DESIGN, vol. 10, 2004, pages 3757 - 3767, XP008060178 *
MORGAN R.A. ET AL: "Retroviral vectors containing putative internal ribosome entry sites: development of a polycistronic gene transfer system and applications to human gene therapy", NUCLEIC ACIDS RESEARCH, vol. 20, 1992, pages 1293 - 1299, XP002910800 *
See also references of EP1756276A4 *
WILSON J.E. ET AL: "Naturally occuring dicistronic cricket paralysis virus RNA is regulated by two internal ribosomal entry sites", MOLL.CELL.BIOL., vol. 20, July 2000 (2000-07-01), pages 4990 - 4999, XP002182115 *

Also Published As

Publication number Publication date
EP1756276A2 (en) 2007-02-28
WO2005102041B1 (en) 2006-11-09
EP1756276A4 (en) 2008-10-29
WO2005102041A2 (en) 2005-11-03

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