WO2005075615A1 - Nouveau procede de production de biodiesel a partir d'huiles et de graisses catalysees par lipase dans un systeme de reaction a milieu organique - Google Patents

Nouveau procede de production de biodiesel a partir d'huiles et de graisses catalysees par lipase dans un systeme de reaction a milieu organique Download PDF

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WO2005075615A1
WO2005075615A1 PCT/CN2004/001372 CN2004001372W WO2005075615A1 WO 2005075615 A1 WO2005075615 A1 WO 2005075615A1 CN 2004001372 W CN2004001372 W CN 2004001372W WO 2005075615 A1 WO2005075615 A1 WO 2005075615A1
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Prior art keywords
lipase
fat
oil
reaction system
organic medium
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PCT/CN2004/001372
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English (en)
French (fr)
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Wei Du
Dehua Liu
Lilin Li
Yuanyuan Xu
Li Wang
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Tsinghua University
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Priority to CA2553653A priority Critical patent/CA2553653C/en
Priority to AT04797388T priority patent/ATE446353T1/de
Priority to EP04797388A priority patent/EP1705238B1/en
Priority to BRPI0418062A priority patent/BRPI0418062B1/pt
Priority to DE602004023761T priority patent/DE602004023761D1/de
Priority to US10/597,123 priority patent/US7550278B2/en
Publication of WO2005075615A1 publication Critical patent/WO2005075615A1/zh
Priority to HK06114093.1A priority patent/HK1094229A1/xx

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
    • C—CHEMISTRY; METALLURGY
    • C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G, C10K; LIQUEFIED PETROLEUM GAS; ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE OR UNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL; FIRELIGHTERS
    • C10L1/00—Liquid carbonaceous fuels
    • C10L1/02—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only
    • C10L1/026—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only for compression ignition
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11C—FATTY ACIDS FROM FATS, OILS OR WAXES; CANDLES; FATS, OILS OR FATTY ACIDS BY CHEMICAL MODIFICATION OF FATS, OILS, OR FATTY ACIDS OBTAINED THEREFROM
    • C11C3/00—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom
    • C11C3/003—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom by esterification of fatty acids with alcohols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P30/00—Technologies relating to oil refining and petrochemical industry
    • Y02P30/20—Technologies relating to oil refining and petrochemical industry using bio-feedstock

Definitions

  • the present invention belongs to the field of bio-oil synthesis, and particularly relates to a new process for producing biodiesel by converting lipase into fat in an organic medium reaction system using an organic solvent as a reaction medium. Background technique
  • biodiesel is a long-chain fatty acid ester produced from bio-oil raw materials through transesterification. It is a new type of pollution-free renewable energy.
  • the combustion performance of biodiesel is comparable to that of traditional petroleum-based diesel. After combustion, harmful substances in the exhaust gas of the engine are reduced by 50% compared with conventional petrochemical diesel. At present, the research and application of biodiesel has received extensive attention.
  • biodiesel is mainly produced by chemical methods, that is, using animal and vegetable fats and some short-chain alcohols (such as methanol or ethanol) to perform transesterification reactions under the action of alkaline or acid catalysts to generate corresponding fatty acid short-chain esters.
  • short-chain alcohols such as methanol or ethanol
  • bio-enzymatic synthesis of biodiesel has the advantages of mild reaction conditions, no pollutant emissions, and free fatty acids and a small amount of water in the oil and fat raw materials do not affect the enzymatic reaction, which is in line with the development direction of green chemistry, so it has been increasingly valued by people.
  • the object of the present invention is to propose a new process for producing biodiesel by converting lipase into fat in an organic medium reaction system.
  • the process uses short-chain alcohol R0H as the reaction acyl acceptor, and uses a relatively hydrophilic organic solvent that does not have a negative effect on the enzyme's due activity as a reaction medium, and uses bioenzyme to catalyze the transesterification reaction of oil and fat raw materials to synthesize biodiesel;
  • the method comprises: loading a short-chain alcohol having a molar ratio of 3: 1 to 6: 1 and fat, 20-200% organic solvent based on fat volume, and 2-30% lipase based on fat mass into an enzyme reactor. Mix well, heat to 20 ° C ⁇ 60 ° C, react for 5-24 hours detailed description
  • the relatively hydrophilic organic solvent is preferably tert-butanol or short-chain fatty acid ester RC00R ', where R and R' are alkyl groups having 1 to 4 carbon atoms.
  • the lipase is a microbial lipase, preferably Lipozyme TL, Lipozyme RM,
  • the fats and oils are biological fats and oils, preferably vegetable fats and oils, animal fats and oils, waste cooking oil, or refining waste.
  • the vegetable oil includes castor oil, rapeseed oil, soybean oil, peanut oil, corn oil, cottonseed oil, rice bran oil, and algae oil.
  • the animal fat is fish oil or lard.
  • R is a fluorenyl group having 1 to 5 carbon atoms, and the short-chain alcohol is preferably methanol, ethanol, propanol, butanol, or pentanol.
  • the reaction molar ratio of the short-chain alcohol to the fat is preferably 3: 1 to 5: 1, and the preferred amount of the organic solvent to be added is 50% to 100% based on the volume of the fat.
  • the enzyme reactor may be a stoppered Erlenmeyer flask or various other suitable enzyme reactors.
  • the heating step is preferably carried out in a reciprocating shaker with automatic temperature control or various biochemical reactors suitable for enzyme-catalyzed reactions.
  • the preferred reaction temperature is 30 ° C ⁇ 50 ° C.
  • the beneficial effects of the invention are to improve the enzyme reaction activity, prolong the service life of the enzyme and significantly improve the yield of biodiesel.
  • These new organic medium reaction systems can promote the dissolution of short chain alcohols such as methanol in fats and oils, and effectively reduce the negative impact of short chain alcohols on the enzymatic reaction activity.
  • the required Short-chain alcohol which can significantly shorten the enzymatic reaction time, improve biodiesel yield, biodiesel yield of more than 94%;
  • these relatively hydrophilic organic solvents can partially dissolve glycerol to a certain extent, can effectively reduce Negative effects of reactant short-chain alcohols and glycerol by-products on the enzyme reaction activity, significantly increase the enzyme reaction activity, effectively improve the "shielding" of glycerol on the pore diameter of the immobilized enzyme, increase the enzyme reaction activity and prolong the service life of the enzyme.
  • Pentyl alcohol and rapeseed oil (rapeseed oil 9.65g) in a molar ratio of 4: 1 and butyl butyrate based on 100% of the fat volume are put into a stoppered triangle bottle and mixed uniformly, and placed in an automatic temperature control After heating in a reciprocating shaker to 50 ° C, the immobilized lipase Lipozyme TL based on 30% of the fat mass was added to start the reaction. After 8 hours, 9.17g of biodiesel was produced, and the yield of biodiesel was 95%.
  • R is a fluorenyl group having 1 to 5 carbon atoms
  • an immobilized lipase with an amount of 2% to 30% based on the mass of fat and oil is added in an appropriate range of 3 ⁇ 4 ⁇ .degree.

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fats And Perfumes (AREA)
  • Liquid Carbonaceous Fuels (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)

Description

在有机介质反应体系中以脂肪酶转化油脂生产生物柴油的新工艺 技术领域
本发明属于生物油脂合成领域，特别涉及利用有机溶剂作为反应 介质的一种在有机介质反应体系中以脂肪酶转化油脂生产生物柴油 的新工艺。 背景技术
油脂工业的新前景一生物柴油是由生物油脂原料通过转酯反应 生成的长链脂肪酸酯类物质， 是一种新型的无污染可再生能源。生物 柴油的燃烧性能可以与传统的石油系柴油媲美，其燃烧后发动机排放 出的尾气里有害物质比传统石化柴油降低了 50%。 目前生物柴油的研 究和应用已经受到了广泛的关注。
目前生物柴油主要是用化学法生产，即用动植物油脂和一些短链 醇 （例如甲醇或乙醇） 在碱性或者酸性催化剂作用下进行转酯反应， 生成相应的脂肪酸短链酯。化学法制备生物柴油存在如下一些不可避 免的缺点： ①油脂原料中的游离脂肪酸和水严重影响反应的进行;② 低碳醇在油脂中溶解性不好，易导致乳化液的形成从而使得后续处理 过程复杂；③工艺要求短链醇用量大大超过反应摩尔比，过量短链醇 的蒸发回流增大过程能耗。
由于利用生物酶法合成生物柴油具有反应条件温和、无污染物排 放、 油脂原料中的游离脂肪酸和少量水不影响酶促反应等优点， 符合 绿色化学的发展方向， 因而日益受到人们的重视。
但同化学方法相比， 生物酶法也存在如下一些亟待解决的问题。 在 Shimada Yuji等人的题为 Enzymatic alcoholysis for biodiesel fuel production and appl ication of the reaction to oil processing ( Journal of Molecular Catalysis B： Enzymatic, 2002, 17 : 133-142 ) 的文献中报道： 甲醇、 乙醇等短链醇在油脂原料 中的溶解性较差， 不利于反应的进行； 其次， 过量醇的存在会导致脂 肪酶严重失活， 故在利用生物酶法生产生物柴油的工艺过程中， 多采 用分批加入短链醇的方式以缓解其对酶的毒害作用。这种方式操作繁 琐， 反应时间较长； 另外， 以短链醇作为反应的酰基受体， 反应过程 中有副产物甘油产生，亲水性的甘油容易附着在固定化酶内孔及外表 面， 从而对酶的活性位点形成 "屏蔽"， 严重影响酶反应活性； 而且， 同化学方法相比， 酶法合成生物柴油的得率相对较低。为改善以上情 况， 一些学者尝试采用有机溶剂反应体系合成生物柴油， 文献 [Shimada Yuji et al. Enzymatic alcoholysis for biodiesel fuel production and application of the reaction to oil processing, Journal of Molecular Catalysis B : Enzymatic, 2002, 17 : 133-142] 报道： 采用一些疏水性较强的有机溶剂如己烷、 环己烷、 石油醚等作 为反应介质。这些疏水性有机溶剂可以很好地溶解油脂， 在一定程度 上能促进反应的进行；但由于这些疏水性较强的有机溶剂并不能有效 溶解甲醇等低碳醇以及副产物甘油， 故对酶反应活性、酶的使用寿命 以及生物柴油得率等并未有明显改善。 发明内容
本发明的目的在于提出一种在有机介质反应体系中以脂肪酶转 化油脂生产生物柴油的新工艺。该工艺是以短链醇 R0H作为反应酰基 受体，用对酶尽应活性没有负面影响的相对亲水的有机溶剂作为反应 介质， 利用生物酶催化油脂原料进行转酯反应合成生物柴油； 其特征 在于： 将醇脂摩尔比为 3: 1〜6： 1的短链醇和油脂、 基于油脂体积 的 20- 200%的有机溶剂和基于油脂质量的 2- 30%的脂肪酶， 装入酶反 应器中混合均匀， 加热至 20°C〜60°C， 反应 5— 24小时， 将油脂原 具体实施方式
在本发明的新工艺中，所述相对亲水的有机溶剂优选为叔丁醇或 短链脂肪酸酯 RC00R' ， 其中 R和 R' 为具有 1一 4个碳原子的烷基。
所述脂肪酶为微生物脂肪酶，优选为 Lipozyme TL, Lipozyme RM,
Novozym 435或它们的混合物。
所述油脂为生物油脂， 优选为植物油脂、 动物油脂、 废食用油或 炼油脚料。
所述植物油脂包括蓖麻油、 菜籽油、 大豆油、 花生油、 玉米油、 棉子油、 米糠油以及藻类油脂。
所述动物油脂为鱼油或猪油。
所述短链醇 R0H中 R为具有 1-5个碳原子的垸基，该短链醇优选 为甲醇、 乙醇、 丙醇、 丁醇或戊醇。
所述短链醇与油脂的反应摩尔比优选为 3： 1〜5： 1，优选的有机溶 剂添加量为基于油脂体积的 50%- 100%。
所述的酶反应器可以是具塞的三角瓶或其它各种合适的酶反应 器。所述的加热步骤优选在可自动控温的往复摇床或适于酶催化反应 的各种生化反应器中进行。 优选的反应温度为 30°C〜50°C。
本发明的有益效果是提高了酶反应活性、延长了酶的使用寿命并 显著提高了生物柴油得率。这些新型有机介质反应体系可以促进甲醇 等短链醇在油脂中的溶解， 有效降低短链醇对酶反应活性的负面影 响； 其次， 在这种有机介质反应体系中， 可以一次性加入反应所需的 短链醇， 从而可显著缩短酶促反应时间、 提高生物柴油得率，生物柴 油得率达 94%以上； 另外， 这些相对亲水的有机溶剂在一定程度上可 以部分溶解甘油，可以有效降低反应物短链醇以及副产物甘油对酶反 应活性的负面影响、 显著提高酶反应活性，有效改善甘油对固定化酶 孔径的 "屏蔽"状况， 提高酶反应活性并延长酶的使用寿命。 卜—面通过实施例来进一步说明本发明。 实施例 1
将摩尔比为 4: 1 的甲醇和菜籽油 （菜籽油 9. 65g) 和基于油脂 体积 100 %的叔丁醇， 装入具塞三角瓶中混合均匀， 并置于可自动控 温的往复摇床中加热至 40°C后，加入基于油脂质量 10%的固定化脂肪 酶 Novozym 435开始反应， 经 6小时后生产出生物柴油 9. 64g， 生物 柴油得率约为 100%。 实施例 2
将摩尔比为 3 : 1的甲醇和大豆油（大豆油 9. 65g)和基于油脂体 积 20%的叔丁醇， 装入具塞三角瓶中混合均匀， 并置于可自动控温的 往复摇床中加热至 2(TC后， 加入基于油脂质量 2%的固定化脂肪酶 Novozym 435开始反应， 经 24小时后生产出生物柴油 9. 17g， 生物柴 油得率为 95%。 实施例 3
将摩尔比为 5 : 1的乙醇和棉籽油 （棉籽油 9. 65g)、 基于油脂体 积 200%的甲酸甲酯， 装入具塞三角瓶中混合均匀， 并置于可自动控 温的往复摇床中加热至 6CTC后，加入基于油脂质量 10%的固定化脂肪 酶 Novozym 435开始反应， 经 12小时后生产出生物柴油 9. lg, 生物 柴油得率为 94%。 实施例 4
将摩尔比为 3 : 1的丁醇和废食用油 （废食用油 9. 65g)、 基于油 脂体积 80 %的叔丁醇， 装入具塞三角瓶中混合均匀， 并置于可自动 控温的往复摇床中加热至 40°C后，加入基于油脂质量 10%的固定化脂 肪酶 Novozym 435开始反应， 经 7小时后生产出生物柴油 9. 65g， 生 物柴油得率为 100%。 实施例 5
将摩尔比为 3: 1的甲醇和大豆油 （大豆油 9. 65g)、 基于油脂休 积 50%的叔丁醇， 装入具塞三角瓶中混合均匀， 并置于可自动控温的 往复摇床中加热至 40°C后， 加入基于油脂质量 20%的固定化脂肪酶 Lipozyme TL开始反应， 经 10小时后生产出生物柴油 9. lg， 生物柴 油得率为 94%。 实施例 6
将摩尔比为 4: 1的戊醇和菜籽油（菜籽油 9. 65g)、 基于油脂体 积 100 %的丁酸丁酯， 装入具塞三角瓶中混合均匀， 并置于可自动控 温的往复摇床中加热至 50°C后，加入基于油脂质量 30%的固定化脂肪 酶 Lipozyme TL开始反应， 经 8小时后生产出生物柴油 9. 17g, 生物 柴油得率为 95%。
实施例 Ί
将摩尔比为 3: 1的乙醇和棉籽油 （棉籽油 9. 65g)、 基于油脂体 积 80%的叔丁醇， 装入具塞三角瓶中混合均匀， 并置于可自动控温的 往复摇床中加热至 40Ό后， 加入基于油脂质量 20%的固定化脂肪酶 Lipozyme RM开始反应， 经 10小时后生产出生物柴油 9. lg， 生物柴 油得率为 94%。 实施例 8 :
将实施例 1中反应后的脂肪酶直接滤出用于下一批次反应，其他 反应条件同实施例 1， 如此将脂肪酶连续回用 10次。 在第 10个反应 比次中， 反应 6小时可生产出生物柴油 9. 62g， 生物柴油得率仍高达 99%。 根据上述实施例， 以叔丁醇、 短链脂肪酸酯 RC00R' ( R、 R' 为 具有 1-4个碳原子数的垸基）作为有机介质反应体系， 以短链醇 R0H
( R为具有 1-5个碳原子数的垸基） 作为反应酰基受体时， 在适宜的 ¾§.度范围下加入基于油脂质量 2%〜30%的固定化脂肪酶 Novozym 435
(来源于 Candida antarctica)^ Lipozyme RM (来源于 Rhizomucor miehei) 或 Lipozyme TL i^M^ Thermomyces lanuginosus) , 不同 生物油脂原料（蓖麻油、 菜籽油、 棉籽油、 废食用油、 大豆油、 鱼油、 猪油、 炼油下脚料、 藻类油脂等)都能被高效转化生成生物柴油。

Claims

权利要求
1.一种在有机介质反应体系中以脂肪酶转化油脂生产生物柴油 的工艺， 该工艺是以短链醇 R0H作为反应酰基受体， 用对酶反应活性 没有负面影响的相对亲水的有机溶剂作为反应介质，利用生物酶催化 油脂原料进行转酯反应合成生物柴油； 其特征在于， 将醇脂摩尔比为 3： 1〜6： 1的短链醇和油脂、 基于油脂体积的 20-200%的有机溶剂和 基于油脂质量的 2- 30%的脂肪酶， 装入酶反应器中混合均匀， 加热至 20° (：〜 60°C， 反应 5— 24小时， 将油脂原料转化生成生物柴油和副产 物甘油。
2.根据权利要求 1 所述的在有机介质反应体系中以脂肪酶转化 油脂生产生物柴油的工艺， 其特征在于， 所述相对亲水的有机溶剂选 自叔丁醇以及短链脂肪酸酯 RC00R' ，其中 R和 R' 为具有 1一 4个碳 原子的烷基。
3.根据权利要求 1 所述的在有机介质反应体系中以脂肪酶转化 油脂生产生物柴油的工艺，其特征在于，所述脂肪酶为微生物脂肪酶。
4. 根据权利要求 3所述的在有机介质反应体系中以脂肪酶转化 油脂生产生物柴油的工艺， 其特征在于， 所述的微生物脂肪酶包括 Lipozyme TL， Lipozyme RM， Novozym 435以及它们的混合物。
5.根据权利要求 1 所述的在有机介质反应体系中以脂肪酶转化 油脂生产生物柴油的工艺， 其特征在于， 所述油脂为生物油脂。
6. 根据权利要求 5所述的在有机介质反应体系中以脂肪酶转化 油脂生产生物柴油的工艺， 其特征在于， 所述的生物油脂包括植物油 脂、 动物油脂、 废食用油以及炼油脚料。
7.根据权利要求 6 所述的在有机介质反应体系中以脂肪酶转化 油脂生产生物柴油的工艺， 其特征在于， 所述植物油脂包括蓖麻油、 菜籽油、 大豆油、 花生油、 玉米油、 棉子油、 米糠油以及藻类油脂。
8.根据权利要求 6 所述的在有机介质反应体系中以脂肪酶转化 袖脂生产生物柴油的工艺，其特征在于，所述动物油脂为鱼油或猪油。
9.根据权利要求 1 所述的在有机介质反应体系中以脂肪酶转化 袖脂生产生物柴油的工艺， 其特征在于， 所述短链醇 R0H中 R为具有 1-5个碳原子的烷基。
10.根据权利要求 9所述的在有机介质反应体系中以脂肪酶转化 袖脂生产生物柴油的工艺， 其特征在于， 所述短链醇为甲醇、 乙醇、 丙醇、 丁醇或戊醇。
11.根据权利要求 1所述的在有机介质反应体系中以脂肪酶转化 袖脂生产生物柴油的工艺， 其特征在于， 所述短链醇与所述油脂的摩 尔比为 3： 1〜5： 1。
12.根据权利要求 1所述的在有机介质反应体系中以脂肪酶转化 油脂生产生物柴油的工艺， 其特征在于， 所述有机溶剂的添加量为基 于油脂体积的 50 %— 100%。
13.根据权利要求 1所述的在有机介质反应体系中以脂肪酶转化 袖脂生产生物柴油的工艺， 其特征在于， 所述的加热步骤在可自动控 §.的往复摇床中进行。
14.根据权利要求 1所述的在有机介质反应体系中以脂肪酶转化 油脂生产生物柴油的工艺， 其特征在于， 所述的反应温度为 30°C〜 50°C。
PCT/CN2004/001372 2004-01-16 2004-11-29 Nouveau procede de production de biodiesel a partir d'huiles et de graisses catalysees par lipase dans un systeme de reaction a milieu organique WO2005075615A1 (fr)

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AT04797388T ATE446353T1 (de) 2004-01-16 2004-11-29 Neues verfahren zur herstellung von biodiesel aus ölen und fetten unter lipasekatalyse in einem organischen medium als reaktionssystem
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BRPI0418062A BRPI0418062B1 (pt) 2004-01-16 2004-11-29 processo para a produção de biodiesel a partir de óleo renovável na presença de catálise por lipase em um sistema de reação em meio orgânico
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