WO2005060727A2 - Decontamination amelioree des semences - Google Patents

Decontamination amelioree des semences Download PDF

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Publication number
WO2005060727A2
WO2005060727A2 PCT/GB2004/005286 GB2004005286W WO2005060727A2 WO 2005060727 A2 WO2005060727 A2 WO 2005060727A2 GB 2004005286 W GB2004005286 W GB 2004005286W WO 2005060727 A2 WO2005060727 A2 WO 2005060727A2
Authority
WO
WIPO (PCT)
Prior art keywords
seeds
chlorine dioxide
compound
solution
generate chlorine
Prior art date
Application number
PCT/GB2004/005286
Other languages
English (en)
Other versions
WO2005060727A3 (fr
Inventor
Keith Warriner
William M. Waites
Original Assignee
The University Of Nottingham
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The University Of Nottingham filed Critical The University Of Nottingham
Publication of WO2005060727A2 publication Critical patent/WO2005060727A2/fr
Publication of WO2005060727A3 publication Critical patent/WO2005060727A3/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B9/00—Preservation of edible seeds, e.g. cereals
    • A23B9/16—Preserving with chemicals
    • A23B9/24—Preserving with chemicals in the form of liquids or solids
    • A23B9/30—Inorganic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B9/00—Preservation of edible seeds, e.g. cereals
    • A23B9/02—Preserving by heating
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B9/00—Preservation of edible seeds, e.g. cereals
    • A23B9/16—Preserving with chemicals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B9/00—Preservation of edible seeds, e.g. cereals
    • A23B9/16—Preserving with chemicals
    • A23B9/18—Preserving with chemicals in the form of gases, e.g. fumigation; Compositions or apparatus therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B9/00—Preservation of edible seeds, e.g. cereals
    • A23B9/16—Preserving with chemicals
    • A23B9/18—Preserving with chemicals in the form of gases, e.g. fumigation; Compositions or apparatus therefor
    • A23B9/22—Preserving with chemicals in the form of gases, e.g. fumigation; Compositions or apparatus therefor in a controlled atmosphere comprising other gases in addition to CO2, N2, O2 or H2O
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
    • A61L2/04—Heat
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
    • A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
    • A61L2/186—Peroxide solutions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
    • A61L2/20—Gaseous substances, e.g. vapours
    • A61L2/202—Ozone
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
    • A61L2/20—Gaseous substances, e.g. vapours
    • A61L2/208—Hydrogen peroxide
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
    • C01B11/00—Oxides or oxyacids of halogens; Salts thereof
    • C01B11/02—Oxides of chlorine
    • C01B11/022—Chlorine dioxide (ClO2)
    • C01B11/023—Preparation from chlorites or chlorates
    • C01B11/024—Preparation from chlorites or chlorates from chlorites

Definitions

  • the present invention relates to methods of reducing contamination of seeds and sprouts cultivated from seeds from human pathogens such as E. coli and Listeria mono cy to genes.
  • Sprouts cultivated from seeds are cultivated at a temperature (in the range of 25-35°C) and a humidity (greater than 80%) optimal for their sprouting and growth.
  • temperatures and humidities provide an ideal environment for breeding of various micro-organisms, including human pathogens.
  • sterile seeds should ideally not be produced as post-harvest contamination could take place in the absence of any competitive microflora.
  • the presence of competitive microflora is critical as they act as a biological buffer to inhibit the growth of pathogens during sprouting.
  • the microflora compete to use the nutrients present thus limiting the nutrients available for pathogen growth. Accordingly, there still exists a need for useful methods for reducing contamination of seeds and sprouts cultivated from seeds that will be eaten that address the aforementioned problems, are practical, and cost effective.
  • the phrase "sprouted seeds" includes sprouted seeds such as beansprouts and other sprouts cultivated from seed and eaten. Examples of the types of seeds that require decontamination are mung beans, alfalfa seeds and cress seeds.
  • the present invention provides a method of reducing contamination of seeds and sprouted seeds, the method comprising treating the seeds with a compound that is activated in use, by an acid generated in situ, to generate chlorine dioxide.
  • the compound activated in use to generate chlorine dioxide preferably comprises sodium chlorite or sodium chlorate.
  • the compound may be provided in the form of a solution or a solid.
  • the compound may be a stable sodium chlorite solution.
  • Most preferably the compound is a stabilised chlorine dioxide (SCD) solution consisting of a highly refined blend of oxy-chloro species containing sodium chlorite.
  • SCD stabilised chlorine dioxide
  • a suitable SCD solution is available as Harvest Wash supplied by Vernagene Ltd.
  • the compound is activated by acid to generate anti-microbial chlorine dioxide.
  • the acid is generated in situ, for example, by bacterial activity during their growth on nutrients released from the germinating seeds .
  • Generation of the acid in situ by the bacterial activity during the germination of the seeds means that the compound is stable before use and therefore readily handled.
  • the release of chlorine dioxide is localised around the bacterial activity and is therefore slower and prolonged compared to the activation by the addition of acid, other than that produced in situ by bacterial activity, which produces a high concentration of chlorine dioxide but this is only present for a short period of time.
  • the seeds are preferably soaked in a solution of the compound for 12 to 36 hours, preferably 18 to 30 hours and most preferably 24 hours.
  • the compound activated in use to generate chlorine dioxide is preferably present in amounts of from at least lOOppm to at least 500ppm or more, a preferred amount is at least lOOppm, and a most preferred amount is 125ppm.
  • the present invention provides a method of reducing contamination of seeds and sprouted seeds, the method comprising the steps, in any order, of i) subjecting the seeds to a heat treatment; and ii) treating the seeds with a decontamination reagent.
  • the heat treatment step is preferably carried out before treatment with the decontamination reagent.
  • the heat treatment step preferably comprises submerging the seeds in water at a temperature of from 95 to 100°C, preferably at boiling point, for a period of less than 20 seconds, preferably 5 to 15 seconds, most preferably 10 to 15 seconds, for example 10 seconds.
  • the heat treatment step is followed by quenching of the seeds in a liquid at a temperature of 0 to 6°C, preferably 4°C or less.
  • the liquid is advantageously a solution of the decontamination reagent.
  • the decontamination reagent is used to quench the seeds after treatment with hot water. This quenching process is advantageous as it rapidly cools the seeds and increases uptake of the decontamination reagent.
  • Treatment with a decontamination reagent can be carried out by one of the following processes:
  • the decontamination reagent may be a compound activated in use, by an acid generated in situ, to generate chlorine dioxide.
  • the compound activated in use to generate chlorine dioxide preferably comprises sodium chlorite or sodium chlorate.
  • the compound may be provided in the form of a solution or a solid.
  • the compound may be a stable sodium chlorite solution.
  • Most preferably the compound is a stabilised chlorine dioxide (SCD) solution consisting of a highly refined blend of oxy-chloro species containing sodium chlorite.
  • SCD stabilised chlorine dioxide
  • a suitable SCD solution is available as Harvest Wash supplied by Vernagene Ltd.
  • the compound is activated by an acid, which is generated in situ, for example, by bacterial activity during their growth on nutrients released from the germinating seeds.
  • Generation of the acid in situ by the bacterial activity during the germination of the seeds means that the compound is stable before use and therefore readily handled.
  • the release of chlorine dioxide is localised around the bacterial activity and is therefore slower and prolonged compared to the activation by the addition of acid, other than that produced in situ by bacterial activity, which produces a high concentration of chlorine dioxide but this is only present for a short period of time.
  • the compound activated in use to generate chlorine dioxide is preferably present in an amount of from at least lOOppm to at least 500ppm or more, a preferred amount is at least lOOppm, most preferably 125ppm.
  • the seeds are preferably soaked in a solution of the compound for 12 to 36 hours, preferably 24 hours.
  • the decontamination reagent may be a compound activated prior to or in use to generate chlorine dioxide.
  • the compound activated prior to or in use to generate chlorine dioxide preferably comprises sodium chlorite.
  • the compound may be provided in the form of a solution or a solid.
  • the compound may be a stable sodium chlorite solution.
  • Most preferably the compound is a stabilised chlorine dioxide (SCD) solution consisting of a highly refined blend of oxy-chloro species containing sodium chlorite.
  • SCD stabilised chlorine dioxide
  • a suitable SCD solution is available as Harvest Wash supplied by Vernagene Ltd.
  • the compound is preferably activated by an acid.
  • the acid may be added to the compound and may, for example, be phosphoric acid or citric acid.
  • the acid When the acid is added to the solution of the compound the acid should be present in amounts of from 3 to 10% w/v or 3 to 10 % v/v.
  • the compound activated prior to or in use to generate chlorine dioxide is preferably present in the solution in an amount of from at least 25ppm to at least 250ppm or more, a preferred amount is at least 25ppm and a most preferred amount is 50ppm.
  • the seeds are soaked in a solution of the compound and acid, added to activate the compound, for a period of from 2 to 10 minutes, preferably 3 to 7 minutes and most preferably 5 minutes.
  • the compound activated in use to generate chlorine dioxide may alternatively comprise a stable chlorine dioxide preparation, which can be activated by spraying.
  • a suitable stable chlorine dioxide preparation is available as Harvest Wash TC supplied by Vernagene Ltd.
  • the seeds or sprouted seeds are sprayed with the compound activated in use to produce chlorine dioxide.
  • the ozone gas is preferably present in air contained in a treatment chamber in an amount of from at least 30ppm to at least 50ppm or more.
  • a most preferred ozone gas content is 30ppm.
  • acetic acid vapour a suitable decontamination reagents including hydrogen peroxide, peracetic acid, ethanol, cetylpyridium chloride or organic acid vapour, for example acetic acid vapour could be used.
  • suitable decontamination reagents including hydrogen peroxide, peracetic acid, ethanol, cetylpyridium chloride or organic acid vapour, for example acetic acid vapour could be used.
  • seeds and sprouted seeds having reduced levels of human pathogens. Further provided are seeds and sprouted seeds obtainable by the methods of the present invention.
  • non- activated Harvest Wash refers to Harvest Wash activated in use by acids generated in situ by bacteria.
  • activated Harvest Wash refers to Harvest Wash activated before use or in use by addition of an acid, e.g. phosphoric acid.
  • TVC provides an indication of microflora naturally present in sprouted seeds and how this microflora is affected by the methods of the present invention.
  • TVC was carried out by plating on tryptic soya agar and incubating at 30°C for 48 hours.
  • Escherichia coli was enumerated on tryptic soya agar containing 30 ⁇ g/ml of kanamycin and incubating at 37°C for 24 hours.
  • the inoculated mung beans were soaked in a Harvest Wash solution (500ml activated prior to use with phosphoric acid) and left for 5 minutes.
  • the decontaminant solution was replaced with sterile water and mung beans germinated.
  • Table 1 Bacterial counts derived from mung bean seeds decontaminated by Harvest Wash activated prior to use with phosphoric acid.
  • TVC is Total Viable Count.
  • Initial Load is bacterial count of inoculated seed before treatment.
  • ND Denotes "not detected”.
  • 0 Denotes mung beans treated in sterile distilled water in the absence of any decontamination reagent.
  • Table 1 The results set out in Table 1 show that levels of lOOppm Harvest Wash, or greater, activated with phosphoric acid reduced the levels of E. coli and L. monocytogenes on inoculated mung beans to below the level of detection.
  • Table 2 Bacterial counts derived from the resultant sprouts of the mung beans decontaminated by Harvest Wash activated prior to use with phosphoric acid.
  • TVC is Total Viable Count. *: Denotes concentration of Harvest Wash activated with phosphoric acid used to decontaminate the beans. ND: Denotes "not detected” . 0: Denotes mung beans treated in sterile distilled water in the absence of decontamination reagent.
  • the inoculated mung beans were submerged in one litre of boiling water for 10-15 seconds. The beans were then rapidly transferred to cold (4°C) activated Harvest Wash solution (500ml activated prior to use with phosphoric acid) and left for 5 minutes. The decontaminant solution was replaced with sterile water and mung beans germinated over four days at 30°C. Counts were performed on the subsequent sprouts.
  • Table 3 Bacterial counts derived from sprouts of inoculated mung beans decontaminated by a combination of hot water and Harvest Wash activated prior to use with phosphoric acid.
  • TVC is Total Viable Count. *: Denotes concentration of Harvest Wash activated with phosphoric acid used to decontaminate the beans. Initial load: Denotes counts recovered from inoculated beans prior to treatment. ND: Denotes "not detected”. 0: Denotes mung beans treated in sterile distilled water in the absence of a decontamination regent.
  • Inoculated mung beans were submerged in boiling water for 15 seconds prior to placing in a chamber containing air into which ozone gas was introduced.
  • the ozone gas concentration was maintained constant in the chamber throughout a 5 minute treatment period.
  • Table 4 Bacterial counts derived from sprouts of mung beans treated with hot water and different ozone gas concentrations.
  • TVC is Total Viable Count. *: Denotes concentration of ozone gas with which the mung beans were treated. 0: Denotes mung beans treated in sterile distilled water in the absence of a decontamination regent. ND: Denotes "not detected”.
  • Table 5 Bacterial counts derived from sprouts of mung beans soaked in non-activated Harvest Wash.
  • TVC is Total Viable Count.
  • NT Denotes “not treated”.
  • ND Denotes "not detected”.
  • HW Denotes Harvest Wash.
  • Treating mung beans with non-activated Harvest Wash is more effective than treating with activated Harvest Wash as seen from comparison with the Comparative Example.
  • Part B - With heat treatment The beans were initially submerged in 1 litre of boiling water for 10 seconds before being soaked in water containing non-activated Harvest Wash for 24 hours at 30 °C. The Harvest Wash solution was then replaced by sterile water and the mung beans sprouting continued for a further 3 days. Counts were then carried out on the sprouts.
  • Table 6 Bacterial counts derived from sprouts of mung beans soaked in non-activated Harvest Wash after an initial hot water treatment.
  • TVC is Total Viable Count.
  • NT Denotes “not treated” .
  • HT Denotes 10 second submersion in boiling water.
  • ND Denotes "not detected”.
  • HW Denotes Harvest Wash.
  • Example 3 shows that non-activated Harvest Wash at levels of at least lOOppm, with or without hot water treatment, provides consistent inactivation of E. coli and L. monocytogenes during mung bean sprouting.
  • NT Denotes “not treated” .
  • ND Denotes “not detected” *: Denotes concentration of non-activated Harvest Wash used to decontaminate the beans.
  • Non-activated Harvest Wash at concentrations of at least lOOppm is sufficient to suppress the growth of E. coli and L. monocytogenes during mung bean sprouting.
  • TVC levels remain fairly high and constant, providing a good biological buffer system.
  • the beans were soaked in distilled water containing a decontamination reagent for 24 hours at 30° C.
  • the decontamination reagent containing solution was then replaced by sterile water and the mung beans sprouting continued for a further 3 days. Counts were then carried out on the sprouts.
  • Table 8 Bacterial counts derived from bean sprouts of mung beans treated with non-activated Harvest Wash or sodium chlorite without heat treatment.
  • NT Denotes "not treated” .
  • ND Denotes "not detected” .
  • Example 5 shows that sodium chlorite is equally as effective as Harvest Wash at inactivating inoculated E. coli and L. monocytogenes.
  • Table 8a Treatment with Hot Water and Ozone Gas.
  • Alfalfa seeds were inoculated by submersion in a cocktail of E. coli and L. monocytogenes (at a concentration of 10 7 cfu/g) for 20 mins. Seeds were dried at room temperature for 24h prior to use. Batches (20g) were decontaminated by soaking in distilled water containing a decontamination reagent for 24 hours at 30°C. The decontamination reagent containing solution was then replaced by sterile water and the alfalfa seed sprouting continued for a further 4 days at 30°C. Counts were then carried out on the sprouts.
  • Table 9 Bacterial counts derived from sprouts of decontaminated alfalfa seeds.
  • NT Denotes "not treated” .

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Inorganic Chemistry (AREA)
  • General Life Sciences & Earth Sciences (AREA)
  • Geology (AREA)
  • Organic Chemistry (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

L'invention concerne un procédé de réduction de la contamination des semences germées. Ce procédé consiste à traiter les semences avec un agent de décontamination. Cet agent de décontamination peut être un composé qui est activé, lors de l'utilisation, par un acide généré in situ, pour obtenir du dioxyde de chlore. L'agent de décontamination peut être de l'ozone ou un autre agent approprié qui contient du peroxyde d'hydrogène. Ce procédé peut également consister à soumettre les semences à un traitement thermique.
PCT/GB2004/005286 2003-12-24 2004-12-17 Decontamination amelioree des semences WO2005060727A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0329929A GB0329929D0 (en) 2003-12-24 2003-12-24 Improvements to decontamination of seeds
GB0329929.4 2003-12-24

Publications (2)

Publication Number Publication Date
WO2005060727A2 true WO2005060727A2 (fr) 2005-07-07
WO2005060727A3 WO2005060727A3 (fr) 2005-09-22

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GB (1) GB0329929D0 (fr)
WO (1) WO2005060727A2 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1752044A2 (fr) * 2005-08-09 2007-02-14 Bi-Oxide Crop Science Llc Procédé pour combattre les maladies fongiques dans des grains et des légumes avec des solutions de chlorite de sodium
EP2757890A1 (fr) * 2011-09-23 2014-07-30 Diversey, Inc. Procédés pour décontaminer des grains de céréales avec du dioxyde de chlore
WO2016074099A1 (fr) * 2014-11-13 2016-05-19 Agri-Neo Inc. Procédé de désinfection de graines comestibles et, en particulier, de graines produisant un mucilage
US10292402B2 (en) 2013-11-22 2019-05-21 Agri-Neo Inc. Method of sanitizing edible seeds, particularly mucilage producing seeds

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3271242A (en) * 1963-03-29 1966-09-06 Alexander R Reed Iii Stable chlorine dioxide composition and method of making same
WO1996039200A1 (fr) * 1995-06-05 1996-12-12 Bernard Technologies, Inc. Compositions biocides a liberation prolongee et leur utilisation
US5922776A (en) * 1995-06-12 1999-07-13 Bernard Technologies, Inc. Sustained release, transparent biocidal compositions
WO2001033961A1 (fr) * 1999-11-09 2001-05-17 Raytec Corporation Procede de conservation de produits cultives

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3271242A (en) * 1963-03-29 1966-09-06 Alexander R Reed Iii Stable chlorine dioxide composition and method of making same
WO1996039200A1 (fr) * 1995-06-05 1996-12-12 Bernard Technologies, Inc. Compositions biocides a liberation prolongee et leur utilisation
US5922776A (en) * 1995-06-12 1999-07-13 Bernard Technologies, Inc. Sustained release, transparent biocidal compositions
WO2001033961A1 (fr) * 1999-11-09 2001-05-17 Raytec Corporation Procede de conservation de produits cultives

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1752044A2 (fr) * 2005-08-09 2007-02-14 Bi-Oxide Crop Science Llc Procédé pour combattre les maladies fongiques dans des grains et des légumes avec des solutions de chlorite de sodium
EP1752044A3 (fr) * 2005-08-09 2008-01-23 Bi-Oxide Crop Science Llc Procédé pour combattre les maladies fongiques dans des grains et des légumes avec des solutions de chlorite de sodium
EP2757890A1 (fr) * 2011-09-23 2014-07-30 Diversey, Inc. Procédés pour décontaminer des grains de céréales avec du dioxyde de chlore
EP2757890B1 (fr) * 2011-09-23 2018-11-07 Diversey, Inc. Procédés pour décontaminer des grains de céréales avec du dioxyde de chlore
US10292402B2 (en) 2013-11-22 2019-05-21 Agri-Neo Inc. Method of sanitizing edible seeds, particularly mucilage producing seeds
WO2016074099A1 (fr) * 2014-11-13 2016-05-19 Agri-Neo Inc. Procédé de désinfection de graines comestibles et, en particulier, de graines produisant un mucilage

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GB0329929D0 (en) 2004-01-28
WO2005060727A3 (fr) 2005-09-22

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