WO2005059505A3 - Método y kit para genotipificación de hla-b27 basados en la pcr en tiempo real - Google Patents

Método y kit para genotipificación de hla-b27 basados en la pcr en tiempo real Download PDF

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Publication number
WO2005059505A3
WO2005059505A3 PCT/ES2004/070105 ES2004070105W WO2005059505A3 WO 2005059505 A3 WO2005059505 A3 WO 2005059505A3 ES 2004070105 W ES2004070105 W ES 2004070105W WO 2005059505 A3 WO2005059505 A3 WO 2005059505A3
Authority
WO
WIPO (PCT)
Prior art keywords
kit
allele
hla
real time
genotypification
Prior art date
Application number
PCT/ES2004/070105
Other languages
English (en)
French (fr)
Other versions
WO2005059505A2 (es
Inventor
Otero Manuel Juan
Ponces Natalia Casamitjana
Borrell Ricardo Pujol
Oriol Roger Colobran
Rivera Eduardo Palou
Canet Maria Rosa Faner
Crusafont Ana Ribera
Original Assignee
Univ Barcelona Autonoma
Otero Manuel Juan
Ponces Natalia Casamitjana
Borrell Ricardo Pujol
Oriol Roger Colobran
Rivera Eduardo Palou
Canet Maria Rosa Faner
Crusafont Ana Ribera
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Barcelona Autonoma, Otero Manuel Juan, Ponces Natalia Casamitjana, Borrell Ricardo Pujol, Oriol Roger Colobran, Rivera Eduardo Palou, Canet Maria Rosa Faner, Crusafont Ana Ribera filed Critical Univ Barcelona Autonoma
Priority to EP04805142A priority Critical patent/EP1741791A2/en
Publication of WO2005059505A2 publication Critical patent/WO2005059505A2/es
Publication of WO2005059505A3 publication Critical patent/WO2005059505A3/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70539—MHC-molecules, e.g. HLA-molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La invención se relaciona con un método para determinar alelos del grupo alélico 27 del locus B del antígeno leucocitario humano (HLA-B27) a partir de una muestra de ácido nucleico y con un kit para Ilevar a cabo este método. El kit comprende cebadores específicos de alelo para usar en la reacción en cadena de la polimerasa (PCR) en tiempo real, y sondas específicas de alelo marcadas fluorescentemente, o un agente intercalador, para detectar señales de fluorescencia. El análisis se completa con la determinación de las temperaturas de fusión de las secuencias amplificadas de ácidos nucleicos. Supera algunas de las limitaciones de los métodos convencionales de tipificación basados en ADN, en términos de tiempo, posibilidad de automatización, y aumento de la resolución, mientras se reduce el número de reacciones PCR. Es útil p.ej. para determinar la predisposición a una enfermedad específica.
PCT/ES2004/070105 2003-12-17 2004-12-03 Método y kit para genotipificación de hla-b27 basados en la pcr en tiempo real WO2005059505A2 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP04805142A EP1741791A2 (en) 2003-12-17 2004-12-03 Method and kit for the genotypification of hla-b27 based on real time pcr

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES200400009 2003-12-17
ES200400009 2003-12-17

Publications (2)

Publication Number Publication Date
WO2005059505A2 WO2005059505A2 (es) 2005-06-30
WO2005059505A3 true WO2005059505A3 (es) 2005-12-29

Family

ID=34684848

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/ES2004/070105 WO2005059505A2 (es) 2003-12-17 2004-12-03 Método y kit para genotipificación de hla-b27 basados en la pcr en tiempo real

Country Status (2)

Country Link
EP (1) EP1741791A2 (es)
WO (1) WO2005059505A2 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2276630B1 (es) * 2005-12-13 2008-06-16 Universitat Autonoma De Barcelona Metodo y kit para genotipificacion de hla-b basados en la pcr en tiempo real.
KR101352470B1 (ko) 2011-05-25 2014-01-16 김현숙 인간 백혈구 항원 ｂ２７과 ｂ５１ 유전자형 진단을 위한 프라이머 및 프로브, 이를 이용한 검사 방법 및 이를 포함하는 검사 키트
CN103255209A (zh) * 2013-03-27 2013-08-21 天津市秀鹏生物技术开发有限公司 用于检测人类白细胞hla-b27抗原基因的引物组及试剂盒
JP2017506500A (ja) * 2013-12-10 2017-03-09 コネクシオ ゲノミクス ピーティーワイ リミテッド 遺伝子アリルを同定するための方法及びプローブ
CN109182491B (zh) * 2018-09-04 2021-10-22 江苏中济万泰生物医药有限公司 一种人类白细胞hla-b*27基因分型引物组及应用
CN110632071A (zh) * 2019-09-18 2019-12-31 南京华银医学检验所有限公司 一种白细胞抗原b27检测方法及其应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1229128A1 (en) 2001-01-31 2002-08-07 Boehringer Mannheim Gmbh New method for genotype determination

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BERNARD PS ET AL: "Homogeneous amplification and variant detection by fluorescent hybridization probes.", CLIN CHEM., vol. 46, no. 2, 2000, pages 147 - 148, XP008072923 *
BON MA ET AL: "Genotyping of HLA-B27 by real-time PCR without hybridization probes.", CLIN CHEM., vol. 46, no. 7, 2000, pages 1000 - 1002, XP008072915 *
FANER R ET AL: "HLA-B27 genotyping by fluorescent resonance emission transfer (FRET) probes in real-time PCR.", HUM IMMUNOL., vol. 65, no. 8, 2004, pages 826 - 838, XP004546057 *
GARCIA-FERNANDEZ S ET AL: "New insights regarding HLA-B27 diversity in the Asian population.", TISSUE ANTIGENS., vol. 58, no. 4, 2001, pages 259 - 262, XP008072921 *
GELSTHORPE AR ET AL: "High-throughput class I HLA genotyping using fluorescence resonance energy transfer (FRET) probes and sequence-specific primer-polymerase chain reaction (SSP-PCR).", TISSUE ANTIGENS., vol. 54, no. 6, 1999, pages 603 - 614, XP008072919 *
OLERUP O ET AL: "HLA-B27 typing by a group-specific PCR amplification.", TISSUE ANTIGENS., vol. 43, no. 4, 1994, pages 253 - 256, XP008072922 *
PAPP AC ET AL: "Single nucleotide polymorphism genotyping using allele-specific PCR and fluorescence melting curves.", BIOTECHNIQUES., vol. 34, no. 5, 2003, pages 1068 - 1072, XP001208020 *
TIEMANN C ET AL: "Rapid DNA typing of HLA-B27 allele by real-time PCR using lightcycler technology.", CLIN LAB., vol. 47, no. 3-4, 2001, pages 131 - 134, XP008072918 *

Also Published As

Publication number Publication date
WO2005059505A2 (es) 2005-06-30
EP1741791A2 (en) 2007-01-10
EP1741791A9 (en) 2009-07-29

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