WO2005049168A3 - Procede et appareil de pre-enrichissement et d'extraction de cellules de sang total densifie - Google Patents

Procede et appareil de pre-enrichissement et d'extraction de cellules de sang total densifie Download PDF

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Publication number
WO2005049168A3
WO2005049168A3 PCT/US2004/038609 US2004038609W WO2005049168A3 WO 2005049168 A3 WO2005049168 A3 WO 2005049168A3 US 2004038609 W US2004038609 W US 2004038609W WO 2005049168 A3 WO2005049168 A3 WO 2005049168A3
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WO
WIPO (PCT)
Prior art keywords
enrichment
whole blood
target cells
cells
processor systems
Prior art date
Application number
PCT/US2004/038609
Other languages
English (en)
Other versions
WO2005049168A2 (fr
Inventor
Steven Gross
Herman Rutner
Original Assignee
Immunivest Corp
Steven Gross
Herman Rutner
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immunivest Corp, Steven Gross, Herman Rutner filed Critical Immunivest Corp
Publication of WO2005049168A2 publication Critical patent/WO2005049168A2/fr
Publication of WO2005049168A3 publication Critical patent/WO2005049168A3/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Hematology (AREA)
  • Mechanical Engineering (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • External Artificial Organs (AREA)

Abstract

La présente invention se rapporte à des réactifs, à des procédés et à des appareils permettant de prétraiter des échantillons sanguins afin d'obtenir des fractions de sang contenant sensiblement toutes les cellules cibles dans des volumes spécimens réduits qui sont adaptés à un enrichissement ultérieur. L'invention a trait des systèmes de traitement d'échantillons, destinés à enrichir des populations cellulaires dont la densité est inférieure à approximativement 1,09 g/ml, à partir d'environ 30 ml de sang total. L'on combine le sang total avec un réactif dense à compatibilité cellulaire, afin d'élever la densité à environ 1,09 g/ml. La centrifugation provoque l'accumulation des cellules cibles et de la plupart des globules blancs contenus dans la couche leuco-plaquettaire dans la partie supérieure, sous une couche de recouvrement tampon à faible densité. Les volumes d'échantillons réduits sont adaptés à des procédés ultérieurs d'enrichissement immunomagnétique ou autres, avant la détection et le dénombrement. Lesdits systèmes de traitement facilitent, conjointement avec d'autres procédures d'enrichissement, la détection de cellules cibles rares, telles que les cellules tumorales circulantes (CTC), lesquelles constituent des marqueurs utiles pour le diagnostic du cancer et le contrôle de la réponse thérapeutique.
PCT/US2004/038609 2003-11-17 2004-11-17 Procede et appareil de pre-enrichissement et d'extraction de cellules de sang total densifie WO2005049168A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52061403P 2003-11-17 2003-11-17
US60/520,614 2003-11-17

Publications (2)

Publication Number Publication Date
WO2005049168A2 WO2005049168A2 (fr) 2005-06-02
WO2005049168A3 true WO2005049168A3 (fr) 2005-09-09

Family

ID=34619495

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/038609 WO2005049168A2 (fr) 2003-11-17 2004-11-17 Procede et appareil de pre-enrichissement et d'extraction de cellules de sang total densifie

Country Status (1)

Country Link
WO (1) WO2005049168A2 (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US8168389B2 (en) 2006-06-14 2012-05-01 The General Hospital Corporation Fetal cell analysis using sample splitting
US8195415B2 (en) 2008-09-20 2012-06-05 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US8986966B2 (en) 2002-09-27 2015-03-24 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
US9174222B2 (en) 2005-04-05 2015-11-03 The General Hospital Corporation Devices and method for enrichment and alteration of cells and other particles

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6913697B2 (en) 2001-02-14 2005-07-05 Science & Technology Corporation @ Unm Nanostructured separation and analysis devices for biological membranes
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
EP2029779A4 (fr) 2006-06-14 2010-01-20 Living Microsystems Inc Utilisation de génotypage snp fortement parallèle pour diagnostic fétal
EP2201134B1 (fr) * 2007-09-19 2018-11-21 The Research Foundation of State University of New York Signatures de l'expression génique dans des échantillons de cellules tumorales enrichis
AU2013215525B2 (en) * 2012-01-31 2017-02-16 Coimmune, Inc. Centrifuge vessels suitable for live cell processing and associated systems and methods
CA2895227A1 (fr) * 2012-12-18 2014-06-26 Robert Chow Preparations a base de cellules sanguines et procedes associes (gen 8)
CN105264127B (zh) 2013-03-15 2019-04-09 Gpb科学有限责任公司 颗粒的片上微流体处理
US20150064153A1 (en) 2013-03-15 2015-03-05 The Trustees Of Princeton University High efficiency microfluidic purification of stem cells to improve transplants
EP2971279B1 (fr) 2013-03-15 2019-11-13 The Trustees of Princeton University Méthodes et dispositifs de purification à rendement élevé
US10976232B2 (en) 2015-08-24 2021-04-13 Gpb Scientific, Inc. Methods and devices for multi-step cell purification and concentration

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5645715A (en) * 1995-05-02 1997-07-08 Biocomp Instruments Inc. Collection tip for fractionating solution gradients

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5645715A (en) * 1995-05-02 1997-07-08 Biocomp Instruments Inc. Collection tip for fractionating solution gradients

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BAKER M.K. ET AL: "Molecular Detection of Breast Cancer Cells in the Peripheral Blood of Advanced-Stage Breast Cancer Patients Using Multimarker Real-Time Reverse Transcription-Polymerase Chain Reaction and a Novel Porous Barrier Density Gradient Centrifugation Technology", CLINICAL CANCER RESEARCH, vol. 9, no. 13, 15 October 2003 (2003-10-15), pages 4865 - 4871, XP002988215 *
GRAHAM M.K. ET AL: "Isolation of Human Polymorphonuclear Leukocytes (Granulocytes) from a Leukocyte-Rich Fraction", THE SCIENTIFIC WORLD JOURNAL, vol. 2, 2002, pages 1393 - 1396, XP009112861 *
HARDINGHAM J.E. ET AL: "Immunobead-PCR: a technique for the detection of circulating tumor cells using immunomagnetic beads and the polymerase chain reaction", CANCER RESEARCH, vol. 53, no. 15, 15 October 2003 (2003-10-15), pages 3455 - 3458, XP000605489 *

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8986966B2 (en) 2002-09-27 2015-03-24 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
US9174222B2 (en) 2005-04-05 2015-11-03 The General Hospital Corporation Devices and method for enrichment and alteration of cells and other particles
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US8372584B2 (en) 2006-06-14 2013-02-12 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US9017942B2 (en) 2006-06-14 2015-04-28 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US8168389B2 (en) 2006-06-14 2012-05-01 The General Hospital Corporation Fetal cell analysis using sample splitting
US9273355B2 (en) 2006-06-14 2016-03-01 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US9347100B2 (en) 2006-06-14 2016-05-24 Gpb Scientific, Llc Rare cell analysis using sample splitting and DNA tags
US8296076B2 (en) 2008-09-20 2012-10-23 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuoploidy by sequencing
US8682594B2 (en) 2008-09-20 2014-03-25 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US8195415B2 (en) 2008-09-20 2012-06-05 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US9353414B2 (en) 2008-09-20 2016-05-31 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US9404157B2 (en) 2008-09-20 2016-08-02 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing

Also Published As

Publication number Publication date
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