WO2005048738A1

WO2005048738A1 - Use of cassia sophera and its aqueous extract for producing foods of reducing symptom of diabetic

Info

Publication number: WO2005048738A1
Application number: PCT/CN2003/001011
Authority: WO
Inventors: Xingbo Feng
Original assignee: Xingbo Feng
Priority date: 2003-11-24
Filing date: 2003-11-24
Publication date: 2005-06-02
Also published as: CN1764390A; AU2003289600A1

Abstract

The invention relates to a use of cassia sophera and its aqueous extract for producing foods of reducing symptom of diabetic, being for making foods from Chinese traditional medicine. The invention possesses the obvious action for reducing blood sugar of diabetic. As long as diabetics take the food of the invention, the action is detectable after two weeks, then it is obvious after four weeks, thereby the cassia sophera and its aqueous extract are preferable for producing foods of reducing symptom of diabetic.

Description

Application of mangosteen and its extract in preparing food for improving diabetes symptoms Technical field

The invention belongs to the field of food use of Chinese medicine.

Background technique

Anemone (Cassia sophera Linn.), A medicinal plant, Cassia spp. According to the "Traditional Chinese Medicine Dictionary": "Amaranth, also known as Amaranth Cassia, is flat, non-toxic, and has a sweet taste. It has the effect of removing phlegm and thirst, making people sleepless, and regulating the effect. It is also known as Antipyretics. Alcoholic extracts from the leaves of Mang Mangosteen have the effect of relaxing the small intestine and bronchial smooth muscle of guinea pigs.

Summary of the invention

The purpose of the present invention is to prepare foods that improve the symptoms of diabetes by using mangosteen and its water extract.

The water extraction method is as follows:

1. Frying method: Water is used as the solvent, and the effective ingredients are extracted by heating and boiling. Generally, it must be extracted 2-4 times. The amount of solvent is generally 6-20 times the amount of awn.

2. Infiltration method: After cold soaking or warm soaking, the mango is passed through the mango with a solvent at an appropriate temperature to dissolve the components of the mango.

3. Immersion method: Cold soaking or warm soaking of amaranth for a large number of solvents, and after the ingredients are dissolved and equilibrated, the extraction solution is directly released.

4. Ultrasonic extraction method: After soaking the awns, put them into ultrasonic waves together with the solvent, and use the effect of ultrasound to dissolve the awns.

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Correction page (Article 91) 5. Microwave extraction method: Put the awn and the solvent in the microwave together, and use the microwave to polarize the molecules to dissolve the components.

When the present invention is used to prepare foods for treating diabetes and improving symptoms, it can be made into teabags or added to foods or beverages, and its water extract can be added with conventional excipients to make oral liquids, tablets, and capsules. Or pills.

The food prepared by the present invention for treating diabetes and improving the symptoms thereof is made of sassafras or its water extract together with auxiliary substances, edible additives or flavors that are beneficial to the human body. The auxiliary substances may be starch-like substances such as Rice and noodles; auxiliary substances can also be oligosaccharides, spirulina, hawthorn, carrot gravy, vitamins, zinc gluconate, dextrin and other substances; edible additives that are good for the human body can be other food additives Chinese medicinal materials or western medicine; the flavoring agent may be a functional sweetener, a flavor enhancer, a flavor, a compound such as a cyclodextrin and the like.

The dose of effective substance can be changed according to the way of taking, the age and weight of the patient, the severity of the disease and other similar factors. The oral dose is 10.0-15.0mg / kg, twice daily.

The food of the invention has better effects of lowering blood sugar, treating diabetes and improving symptoms. Under the condition of normal diet, patients can consciously maintain blood sugar stability, obtain non-drug treatment effects of diabetes, and solve nutritional balance and metabolism problems of patients with diabetes.

detailed description

The beneficial effects of the food prepared by the application of the present invention are further explained through test examples. These test examples include pharmacodynamic tests and clinical efficacy observation tests.

Test example 1 Effect of limulus mandshurica extract on hyperglycemic animal model in Wistar rats 1.1 Test materials

Medicine Mang Mang extract was extracted by the New Drug Research Institute of Institute of Regenerative Medicine Science of Jilin University

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Correction page (Article 91) Supply, batch number: 20010708; Xiaoke pills are produced by Guangzhou No. 1 Chinese Medicine Factory, approval number: (1994) No. 11146.

Animal Wistar rats, male and female, weighing 195g-240g, provided by the Experimental Animal Department of Jilin University, quality certificate number: Yidongzi No. 10-5110; main reagents and instruments Streptozotocin STZ produced by the American sigma chemical company , Lot Number: 89H0604

Blood glucose test strip produced by Beijing Yicheng Bioelectronic Technology Co., Ltd.

JPS-III fast blood glucose tester produced by Beijing Yicheng Bioelectronic Technology Co., Ltd.

Centrifuge Beijing Medical Centrifuge Factory, Model: LDZ5-2.

Fully automatic biochemical analyzer, produced by Hitachi, Japan, Model: Hitachi 7170A.

1.2 Test method

Test conditions and methods:

Since the introduction of Wistar rats by the Animal Department of Jilin University, they have been kept in the animal room of the Basic Medical College of Jilin University, room temperature 22-25 ° C, humidity 30-60%, feed composition: corn meal 39%, bean cake noodle 25%, wheat bran noodle 14 %, Sorghum noodles 10%, bone meal 2.5%, fish meal 5%, yeast 3.5%, and additives 1%.

Take the mango extract powder to prepare 5.2% (g / v), 2.6% (g / v), 1.3% (g / v) large, medium and small doses of test solution. Xiaoke pill is formulated into a test solution with a concentration of 20% (g / v).

110 Wistar rats, male and female, were introduced from the Animal Department of Jilin University. They were acclimated in the room for 1 week, fasted for 4 hours, and weighed, measured for blood glucose, and marked. 100 male and female rats with good blood glucose levels in the normal range were selected for experiments, 10 of them were left as normal control groups, and 90 Wistar rats were modeled with streptozotocin STZ at 0.054 g / kg The streptozotocin STZ was formulated into a 2.0% solution with 0.05 mol / L citric acid solution PH = 4.5, and is now used.

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Correction page (Article 91) After fasting for 16 hours without fasting, rats were injected with streptozotocin STZ solution once intraperitoneally according to their weight. On the eighth day after fasting for 4 hours, the blood glucose was measured, and a blood glucose value of more than 10 mmol / L was established as a model. Compared with the normal control group, there was a significant difference P <0.001. Fifty-four hyperglycemic model animals were randomly divided into five groups, with 10 to 11 animals in each group. The groups are: normal control group, model group, Xiaoke Wan group 2.0g / kg, which is equivalent to 4 times the clinical dosage; mango extract large dose group 0.62g / kg, medium dose group 0.31g / kg, low dose group 0.15g / kg, equivalent to 8, 4, or 2 times the clinical dosage.

The model group was given 10 ml / kg of water, and each administration group was given 10 ml / kg of test solution. Gavage is administered around 9am every day for four consecutive weeks. Blood glucose and body weight are measured weekly, and the dose is calculated once based on the new weight. Animals were sacrificed after the fourth week of administration, blood tests were performed for blood routine, blood biochemical indicators, blood rheology and insulin content, and pancreas were taken for histopathological examination. The results obtained by each group are represented by the mean (Ϊ) soil standard deviation (S), and the results are compared with the model group to perform a t-test between the groups to determine their significance. 1.3 Test results

1.3.1 Impact on Blood Sugar

At the third week after administration, the blood glucose of the mango extract in the high-dose group, the middle-dose group and the model group significantly decreased, P <0.05. At the fourth week, the blood glucose levels of the large and medium doses of the mango extract were lowered. Compared with the model group, the blood glucose level was significantly statistically significant P <0.05, see Table 1.

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Correction page (Article 91) § Composition

the amount

Effect of Mang Mango Extract on Blood Glucose (mmol / L) in Hyperglycemic Model Rats (± s) Normal Control Model Model Xiaoke Pill Group Medium Dose Group Low Dose Group 10ml / kg 2. Og / kg 0.62 g / kg 0.31g / kg 0.15g / kg

10 8 9 10 9 8 6.11 ± 0.53 17.52 ± 2.40 17.22 ± 3, 22 17.04 ± 3.34 17.10 ± 2.63 16.34 ± 3.32 one week 6.19 ± 0.50 17.15 ± 2.23 16.84 ± 3.06 15.54 ± 3.23 15.10 ± 2.36 15.72 ± 3.15 two weeks before administration 6.12 ± 0 · 41 15.85 ± 3.02 14.57 ± 3.02 13.59 ± 2.60 14.06 ± 2.53 15.18 ± 3.23 three weeks 6.22 ± 0.31 14.05 ± 3.52 11.97 ± 2.53 10.93 ± 2.58 * 10.71 ± 2.72 * 13.05 ± 3.26 four weeks 5.89 ± 0.46 13.80 ± 4.16 9.38 ± 2.83 * 9.07 ± 2.78 * 9.45 ± 1.98 * 11.71 ± 3.53 Compared with the model group, * P <0.05

Test Example 2 Clinical observation of the food of the present invention in the treatment of diabetic patients 2.1-General information See Table 2 Table 2

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Correction page (; Article 91) 2.2 Treatment results

2.2.1 The glucose-lowering effect of the present invention is extremely significant

It can be clearly seen from Table 2 that these 25 diabetic patients consumed it for 28 days. Except for 2 patients who had diarrhea and general discomfort, the others had a significant hypoglycemic effect. After taking and comparing the blood glucose level, the average blood glucose was reduced by 2.0mmol. / L, reduced by 14.1mmol / L in severe patients. After t-test analysis, the t-value = 3.161, and at the degrees of freedom N = N-1 = 23-1 = 22, p = 0.01 and t = 2.819, so the effect of reducing glucose is extremely significant.

2.2.2 The longer you drink, the better the effect

2.2.2.1 The results of one week of drinking are shown in Table 3:

Analysis of hypoglycemic effect after drinking for a week

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Correction page (Article 91) Test 1 according to Table 3, the results are: t = l, 962, when the degree of freedom Nl = 22-l = 21, t = 2.080 for p-0.05, t = 1.721 for p == 0.10, so although drinking one week The effect is not significant. 2.2.2.2 The analysis of the effect of drinking for two weeks is shown in Table 4:

Table 4 Analysis of hypoglycemic effect after drinking for two weeks

t test results, t = 2,2637, when the degrees of freedom N = N-1 = 22-1 = 21, p = 0.05, t = 2.080, p = (U0's t = 2.831, so the difference is significant, that is, the effect is obvious .

2.2.2.3 The effect analysis after drinking for three weeks is shown in Table 5:

Correction page (Article 91) Table 5 Analysis of hypoglycemic effect after drinking for three weeks

t test result, t = 2.5043, when the degree of freedom N = N-1 = 19-1 = 18, p = 0.05 t = 2.101 p = 0.01 t = 2.878, so the effect is significant.

2.2.2.4 The analysis of the effects of drinking for four weeks is shown in Table 6:

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Correction page (Article 91) Analysis of hypoglycemic effect after drinking

According to the t test result, t = 3.161, when the degrees of freedom N = N-1 = 22-1 = 22, p = 0.05 and t = 2.074, p = 0.01 and t = 2.819, so the effect is extremely significant.

According to the analysis, the diabetic patients drink the invention, the longer the drinking time, the more obvious the effect, that is, drinking for one week is basically ineffective, drinking for two weeks shows a hypoglycemic effect, drinking for three weeks has a significant hypoglycemic effect, and drinking for four weeks is extremely significant .

2.2.3 Analysis of disease duration and drinking effect

The 23 diabetes patients drinking the invention in Table 1 were divided into two groups: those with disease for more than 5 years as a group, those with disease for less than 4 years as a group, and statistical analysis was performed, and the results were as follows:

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Correction page (Article 91) 2.2.3.1. The analysis of the effect for more than 5 years is shown in Table 7.

Table 7 Analysis of drinking effects for more than 5 years

t test result, t = 1.782, when the degrees of freedom N = N_ 1 = 13—1 = 12, t == 2.197 at p = 0.05, and t = 1.782 at p = 0.01, so the effect is not significant.

2.2.3.2. Table 8 shows the analysis of drinking effects for patients under 4 years of illness.

Analysis of drinking effects under the age of 4 years

t test result, t = 2.966, when the degree of freedom N = N—1-10—1 = 9, p = 0.05 of 2.262, p = 0.01 of t = 3.250, so the effect is significant.

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Correction page (Article 91) 2.3 Conclusion

1. Amaranth has a significant effect on reducing blood sugar in patients with diabetes.

2. Diabetic patients can take two weeks to drink it, and the effect is obvious around four weeks.

3. The illness time is shorter (less than 4 years) and longer than that (more than 5 years).

The following examples further illustrate the preparation of the food of the present invention.

Example 1

A health-care oral liquid containing mangosteen water extract to improve the symptoms of diabetes 2.0g of mangosteen water extract, 10000ml of water, mixed into 100 bottles, each 100ml, containing 20mg of mangosteen extract .

Example 2

Teabag containing mangosteen which can improve symptoms of diabetes

Mangosteen 20.0g, kudingcha was pulverized into 4980g of L5 ~ 2mm pieces, and the two were thoroughly mixed and put into a food bag to make 500 bags. Each bag weighed 10g and contained mangosteen 40mg.

Example 3

Beverage containing amaranth extract to improve diabetes symptoms

Water extract of mangosteen 10g, mixed with 1000L of prickly pear juice and packed into 1000 bottles, 1L per bottle, each bottle contains 10mg of water extract from mangosteen.

Example 4

Food containing mangosteen to improve diabetes symptoms

Mangosteen water extract 10g, flour 1000g, flavor lg, yeast lg, 2g salt, as raw materials, add water and flour, proof, bake, cool, and package.

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Correction page (Article 91)

Claims

Request for Rights

And stalk in the preparation of food to improve the symptoms of diabetes

Application of Amaranth water extract in the preparation of food for treating diabetes and improving its symptoms °

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Correction page (Article 91)