WO2005033337A1 - Oligonucleotide a utiliser pour detecter une bacterie aerobie sporogene, procede de determination et trousse de determination pour bacterie aerobie sporogene l'utilisant - Google Patents

Oligonucleotide a utiliser pour detecter une bacterie aerobie sporogene, procede de determination et trousse de determination pour bacterie aerobie sporogene l'utilisant Download PDF

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Publication number
WO2005033337A1
WO2005033337A1 PCT/JP2004/014240 JP2004014240W WO2005033337A1 WO 2005033337 A1 WO2005033337 A1 WO 2005033337A1 JP 2004014240 W JP2004014240 W JP 2004014240W WO 2005033337 A1 WO2005033337 A1 WO 2005033337A1
Authority
WO
WIPO (PCT)
Prior art keywords
bacterium
spore
oligonucleotide
bacteria
spore aerobic
Prior art date
Application number
PCT/JP2004/014240
Other languages
English (en)
Japanese (ja)
Inventor
Mikiko Yamanaka
Shoichi Murata
Original Assignee
Suntory Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Suntory Limited filed Critical Suntory Limited
Priority to CN2004800288051A priority Critical patent/CN1863925B/zh
Priority to KR1020067007783A priority patent/KR101113974B1/ko
Publication of WO2005033337A1 publication Critical patent/WO2005033337A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Abstract

Cette invention concerne un oligonucléotique qui contient une séquence de base spécifique du gène de l'ARN ribosomique 16S d'une bactérie aérobie sporogène ou une séquence complémentaire à ladite séquence de base. La séquence de base spécifique susmentionnée est une séquence de base représentée par n'importe quelle séquence comprise entre SEQ ID NO:1 et SEQ ID NO:6. Cet oligonucléotide est utilisé comme amorce dans une réaction en chaîne de la polymérase, un acide nucléique préparé à partir d'une bactérie examinée à déterminer étant utilisé comme spécimen, lequel spécimen sert de modèle. Il est ainsi possible de déterminer si une bactérie examinée est une bactérie aérobie sporogène ou non sur la base de l'amplification d'un fragment d'ADN spécifique.
PCT/JP2004/014240 2003-10-03 2004-09-29 Oligonucleotide a utiliser pour detecter une bacterie aerobie sporogene, procede de determination et trousse de determination pour bacterie aerobie sporogene l'utilisant WO2005033337A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN2004800288051A CN1863925B (zh) 2003-10-03 2004-09-29 用于好气性产芽孢细菌鉴定的寡核苷酸以及使用此寡核苷酸的好气性产芽孢细菌的鉴定方法和鉴定试剂盒
KR1020067007783A KR101113974B1 (ko) 2003-10-03 2004-09-29 유포자 호기성 세포의 판정에 사용되는올리고뉴클레오티드, 이를 이용한 유포자 호기성 세균의판정방법 및 판정 키트

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2003346167A JP4242244B2 (ja) 2003-10-03 2003-10-03 有胞子好気性細菌の判定に用いられるオリゴヌクレオチド、これを用いた有胞子好気性細菌の判定方法および判定キット
JP2003-346167 2003-10-03

Publications (1)

Publication Number Publication Date
WO2005033337A1 true WO2005033337A1 (fr) 2005-04-14

Family

ID=34419495

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2004/014240 WO2005033337A1 (fr) 2003-10-03 2004-09-29 Oligonucleotide a utiliser pour detecter une bacterie aerobie sporogene, procede de determination et trousse de determination pour bacterie aerobie sporogene l'utilisant

Country Status (5)

Country Link
JP (1) JP4242244B2 (fr)
KR (1) KR101113974B1 (fr)
CN (1) CN1863925B (fr)
TW (1) TWI342338B (fr)
WO (1) WO2005033337A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006014022A1 (fr) * 2004-08-04 2006-02-09 Suntory Limited Instrument de détection de bactérie, procédé de détection de bactérie et kit de détection de bactérie
EP1975257A1 (fr) * 2007-03-30 2008-10-01 GC Corporation Procédé pour la mesure du nombre de Lactobacillus oraux, et ensemble d'amorces-sonde de PCR utilisé pour ce procédé

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5279204B2 (ja) * 2007-06-04 2013-09-04 サントリーホールディングス株式会社 細菌検出器具、細菌検出方法および細菌検出キット

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0994100A (ja) * 1995-07-03 1997-04-08 Higeta Shoyu Co Ltd 細菌同定法及びキット
JPH11137259A (ja) * 1997-11-05 1999-05-25 Mitsui Norin Co Ltd 細菌同定のためのオリゴヌクレオチドおよびそれを用いた細菌の同定方法
JP2002051783A (ja) * 2000-08-09 2002-02-19 Denso Corp 真正細菌の検出・定量法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0994100A (ja) * 1995-07-03 1997-04-08 Higeta Shoyu Co Ltd 細菌同定法及びキット
JPH11137259A (ja) * 1997-11-05 1999-05-25 Mitsui Norin Co Ltd 細菌同定のためのオリゴヌクレオチドおよびそれを用いた細菌の同定方法
JP2002051783A (ja) * 2000-08-09 2002-02-19 Denso Corp 真正細菌の検出・定量法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HANSEN B.M. ET AL.: "Polymerase chain reaction assay for the detection of Bacillus cereus group cells", FEMS MICROBIOL. LETT., vol. 202, no. 2, 21 August 2001 (2001-08-21), pages 209 - 213, XP002984212 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006014022A1 (fr) * 2004-08-04 2006-02-09 Suntory Limited Instrument de détection de bactérie, procédé de détection de bactérie et kit de détection de bactérie
EP1975257A1 (fr) * 2007-03-30 2008-10-01 GC Corporation Procédé pour la mesure du nombre de Lactobacillus oraux, et ensemble d'amorces-sonde de PCR utilisé pour ce procédé
US8133677B2 (en) 2007-03-30 2012-03-13 Gc Corporation Method for measuring the number of oral Lactobacillus, and a PCR primers-probe set used for the same

Also Published As

Publication number Publication date
TWI342338B (en) 2011-05-21
JP2005110530A (ja) 2005-04-28
KR101113974B1 (ko) 2012-03-06
CN1863925B (zh) 2012-09-05
JP4242244B2 (ja) 2009-03-25
KR20060076786A (ko) 2006-07-04
CN1863925A (zh) 2006-11-15
TW200525039A (en) 2005-08-01

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