WO2005001475A3 - Appareil et procede de detection d'organismes vivants microscopiques au moyen de bacteriophage - Google Patents

Appareil et procede de detection d'organismes vivants microscopiques au moyen de bacteriophage Download PDF

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Publication number
WO2005001475A3
WO2005001475A3 PCT/US2004/011285 US2004011285W WO2005001475A3 WO 2005001475 A3 WO2005001475 A3 WO 2005001475A3 US 2004011285 W US2004011285 W US 2004011285W WO 2005001475 A3 WO2005001475 A3 WO 2005001475A3
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WO
WIPO (PCT)
Prior art keywords
bacteriophage
sample
phage
test
bacteria
Prior art date
Application number
PCT/US2004/011285
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English (en)
Other versions
WO2005001475A2 (fr
Inventor
Kent Voorhees
John Rees
John H Wheeler
Original Assignee
Kent Voorhees
John Rees
John H Wheeler
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2003/011253 external-priority patent/WO2003087772A2/fr
Application filed by Kent Voorhees, John Rees, John H Wheeler filed Critical Kent Voorhees
Priority to JP2006532405A priority Critical patent/JP4578478B2/ja
Priority to CN2004800163658A priority patent/CN101375163B/zh
Priority to EP04775884A priority patent/EP1613965A2/fr
Publication of WO2005001475A2 publication Critical patent/WO2005001475A2/fr
Publication of WO2005001475A3 publication Critical patent/WO2005001475A3/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé de détection d'une ou de plusieurs bactéries cible (14) dans un échantillon brut qui consiste: à ajouter un ou des bactériophages (18,102) spécifiques à chaque bactérie dans un échantillon brut, à incuber l'échantillon test et, à appliquer cet échantillon test à un substrat (64,220) qui change de couleur si le bactériophage ou la substance biologique associés à ce Bactériophage est présente. Le substrat contient des anticorps (44,228) qui se lient spécifiquement à chaque phage. La bactérie présente dans l'échantillon test peut être lysée par addition d'une lysozyme (22) microbienne à l'échantillon exposé aux bactériophages. Les phages parents (102) sont marqués (105) de façon qu'ils puissent être séparés du phage de descendance (106) avant le processus de détection. Le phage peut-être dissocié (94,124) après le processus d'incubation et l'échantillon peut être testé (99, 116) pour détecter la présence de protéines de capside individuelles (97, 72) ou d'acides nucléiques de phage (74). Cette invention peut être utilisée (140) pour tester la résistance aux antibiotiques de bactéries cible.
PCT/US2004/011285 2003-04-10 2004-04-12 Appareil et procede de detection d'organismes vivants microscopiques au moyen de bacteriophage WO2005001475A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2006532405A JP4578478B2 (ja) 2003-04-10 2004-04-12 バクテリオファージを使用した微生物を検出するための装置および方法
CN2004800163658A CN101375163B (zh) 2003-04-10 2004-04-12 用噬菌体检测微观活生物体的装置与方法
EP04775884A EP1613965A2 (fr) 2003-04-10 2004-04-12 Appareil et procede de detection d'organismes vivants microscopiques au moyen de bacteriophage

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
PCT/US2003/011253 WO2003087772A2 (fr) 2002-04-12 2003-04-10 Procede de detection de faibles concentrations d'une bacterie cible qui utilise des phages pour infecter des cellules bacteriennes cibles
USPCT/US03/11253 2003-04-10
US10/249,452 US7166425B2 (en) 2002-04-12 2003-04-10 Method for detecting low concentrations of a target bacterium that uses phages to infect target bacterial cells
US10/249,452 2003-04-10
US54443704P 2004-02-13 2004-02-13
US60/544,437 2004-02-13
US55796204P 2004-03-31 2004-03-31
US60/557,962 2004-03-31

Publications (2)

Publication Number Publication Date
WO2005001475A2 WO2005001475A2 (fr) 2005-01-06
WO2005001475A3 true WO2005001475A3 (fr) 2005-03-03

Family

ID=33556564

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/011285 WO2005001475A2 (fr) 2003-04-10 2004-04-12 Appareil et procede de detection d'organismes vivants microscopiques au moyen de bacteriophage

Country Status (2)

Country Link
EP (1) EP1613965A2 (fr)
WO (1) WO2005001475A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12006531B2 (en) 2019-11-05 2024-06-11 Laboratory Corporation Of America Holdings Methods and systems for rapid detection of microorganisms using infectious agents

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US7524625B2 (en) 2000-10-30 2009-04-28 Sru Biosystems, Inc. Real time binding analysis of antigens on a biosensor surface
AU2003284228A1 (en) * 2002-10-15 2004-05-04 Dwight L. Anderson Assays to detect or quantify bacterial or viral pathogens and contaminants
US20080241819A1 (en) * 2006-10-31 2008-10-02 Microphage (Tm) Incorporated Method and apparatus for enhanced bacteriophage-based diagnostic assays by selective inhibition of potential cross-reactive organisms
GB0503172D0 (en) * 2005-02-16 2005-03-23 Enigma Diagnostics Ltd Detection method
MX2007010571A (es) * 2005-03-04 2008-03-11 Blaze Venture Technologies Ltd Metodo y dispositivo para muestreo bacteriano.
GB2436616A (en) * 2006-03-29 2007-10-03 Inverness Medical Switzerland Assay device and method
EP2142667B1 (fr) * 2007-04-05 2014-12-03 Sequella, Inc. Procédés perfectionnés et compositions pour déterminer l'état pathogène des agents infectieux
US20100240025A1 (en) * 2007-06-15 2010-09-23 Timothy Read Bacteriophage with Enhanced Lytic Activity
CN101329342A (zh) * 2007-06-22 2008-12-24 王霁 检测结核菌和抗结核药物敏感性的方法及试剂盒
CN101871939B (zh) * 2010-07-09 2014-09-10 北京易斯威特生物医学科技有限公司 检测试剂盒及其应用方法
WO2012158041A1 (fr) * 2011-05-18 2012-11-22 Rna Holding B.V. Procédés et kits diagnostiques pour déterminer la présence d'un microorganisme dans un échantillon
US10519483B2 (en) 2012-02-21 2019-12-31 Laboratory Corporation Of America Holdings Methods and systems for rapid detection of microorganisms using infectious agents
US10913934B2 (en) 2013-02-21 2021-02-09 Laboratory Corporation Of America Holdings Methods and systems for rapid detection of microorganisms using infectious agents
FR3024465B1 (fr) * 2014-07-30 2018-03-23 Biomerieux Caracterisation de micro-organismes par maldi-tof
JP2020513764A (ja) * 2016-12-30 2020-05-21 クイデル コーポレーション ファージ媒介性イムノアッセイ及び抗生物質又はプロバイオティック薬剤に対する細菌の感受性を決定するための方法
CN106995805A (zh) * 2017-03-26 2017-08-01 海南大学 一种溶菌酶标记的工程化噬菌体快速检测微生物
EP3692160A1 (fr) * 2017-10-02 2020-08-12 Quidel Corporation Procédé de détection à base de phages pour le test de susceptibilité antimicrobienne et l'identification d'espèces bactériennes
EP3987015A1 (fr) 2019-06-21 2022-04-27 Laboratory Corporation of America Holdings Procédés de production de bactériophages mutants pour la détection de listeria
CN114729332A (zh) 2019-08-26 2022-07-08 美国控股实验室公司 用于使用重组繁殖缺陷性指示噬菌体检测微生物的装置和方法
MX2022003092A (es) 2019-09-11 2022-06-08 Laboratory Corp America Holdings Metodos y sistemas para la deteccion rapida de microorganismos utilizando agentes infecciosos recombinantes para expresar una subunidad indicadora.

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US20030027241A1 (en) * 2001-07-20 2003-02-06 Sayler Gary S. Bioluminescent biosensor device
WO2003087772A2 (fr) * 2002-04-12 2003-10-23 Colorado School Of Mines Procede de detection de faibles concentrations d'une bacterie cible qui utilise des phages pour infecter des cellules bacteriennes cibles

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12006531B2 (en) 2019-11-05 2024-06-11 Laboratory Corporation Of America Holdings Methods and systems for rapid detection of microorganisms using infectious agents

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Publication number Publication date
WO2005001475A2 (fr) 2005-01-06
EP1613965A2 (fr) 2006-01-11

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