WO2005001063A3 - Polynucleotides aptes a la circularisation dependante de la cible et a la liaison topologique - Google Patents

Polynucleotides aptes a la circularisation dependante de la cible et a la liaison topologique Download PDF

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Publication number
WO2005001063A3
WO2005001063A3 PCT/US2004/020589 US2004020589W WO2005001063A3 WO 2005001063 A3 WO2005001063 A3 WO 2005001063A3 US 2004020589 W US2004020589 W US 2004020589W WO 2005001063 A3 WO2005001063 A3 WO 2005001063A3
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WO
WIPO (PCT)
Prior art keywords
target
polynucleotides
circularization
polynucleotides capable
dependent
Prior art date
Application number
PCT/US2004/020589
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English (en)
Other versions
WO2005001063A2 (fr
Inventor
Sergei A Kazakov
Anne Dallas
Tai-Chih Kuo
Brian H Johnston
Original Assignee
Somagenics Inc
Sergei A Kazakov
Anne Dallas
Tai-Chih Kuo
Brian H Johnston
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Somagenics Inc, Sergei A Kazakov, Anne Dallas, Tai-Chih Kuo, Brian H Johnston filed Critical Somagenics Inc
Priority to JP2006517706A priority Critical patent/JP2007524395A/ja
Priority to US10/561,691 priority patent/US20070105108A1/en
Priority to EP04777154A priority patent/EP1644531A4/fr
Publication of WO2005001063A2 publication Critical patent/WO2005001063A2/fr
Publication of WO2005001063A3 publication Critical patent/WO2005001063A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/122—Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne des polynucléotides à régulation allostérique capables à circularisation dépendant du cible et liaison topologique à une molécule-cible d'acide nucléique. Ces polynucléotides comportent une séquence-cible de liaison et un élément régulateur bloquant la circularisation en l'absence de la liaison à la cible. Ces polynucléotides peuvent comporter un domaine catalytique, permettant la circularisation par catalyse lorsque la séquence-cible de liaison du polynucléotide est liée à la cible. Des polynucléotides à liaison topologique peuvent être utilisés pour détecter des molecules-cibles ou pour bloquer la transcription ou la traduction de la cible.
PCT/US2004/020589 2003-06-25 2004-06-25 Polynucleotides aptes a la circularisation dependante de la cible et a la liaison topologique WO2005001063A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2006517706A JP2007524395A (ja) 2003-06-25 2004-06-25 標的依存性の環状化及びトポロジー的結合が可能なポリヌクレオチド
US10/561,691 US20070105108A1 (en) 2003-06-25 2004-06-25 Polynucleotides capable of target-depedent circularization and topological linkage
EP04777154A EP1644531A4 (fr) 2003-06-25 2004-06-25 Polynucleotides aptes a la circularisation dependante de la cible et a la liaison topologique

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48265303P 2003-06-25 2003-06-25
US60/482,653 2003-06-25

Publications (2)

Publication Number Publication Date
WO2005001063A2 WO2005001063A2 (fr) 2005-01-06
WO2005001063A3 true WO2005001063A3 (fr) 2005-07-14

Family

ID=33551999

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/020589 WO2005001063A2 (fr) 2003-06-25 2004-06-25 Polynucleotides aptes a la circularisation dependante de la cible et a la liaison topologique

Country Status (4)

Country Link
US (1) US20070105108A1 (fr)
EP (1) EP1644531A4 (fr)
JP (1) JP2007524395A (fr)
WO (1) WO2005001063A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006108422A2 (fr) * 2005-04-12 2006-10-19 In Situ Rcp A/S Under Founding Procedes de production d'oligonucleotides
CN101213310A (zh) 2005-04-12 2008-07-02 Novia公司名下的现场Rcp公司 用于产生寡核苷酸的方法
JP5296328B2 (ja) * 2007-05-09 2013-09-25 独立行政法人理化学研究所 １本鎖環状ｒｎａおよびその製造方法
WO2009073218A1 (fr) * 2007-12-04 2009-06-11 Somagenics Inc. Sondes d'hybridation supérieures et leurs procédés d'utilisation dans la détection de cibles polynucléotidiques

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5118801A (en) * 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
FR2675803B1 (fr) * 1991-04-25 1996-09-06 Genset Sa Oligonucleotides fermes, antisens et sens et leurs applications.
AU756301B2 (en) * 1997-08-20 2003-01-09 Somagenics, Inc. Antisense and antigene therapeutics with improved binding properties and methods for their use
FR2813085A1 (fr) * 2000-08-18 2002-02-22 Aventis Pharma Sa Systeme de regulation in vivo de l'expression d'un transgene par inhibition conditionnelle
US7125660B2 (en) * 2000-09-13 2006-10-24 Archemix Corp. Nucleic acid sensor molecules and methods of using same
WO2002022882A2 (fr) * 2000-09-13 2002-03-21 Archemix Corporation Biocapteur d'acide nucleique active par une cible et ses procedes d'utilisation

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CARRIERO S. ET AL.: "Template-mediated synthesis of lariat RNA and DNA", J. ORG. CHEM., vol. 68, 2003, pages 8328 - 8338, XP002985864 *
FLYNN-CHARLEBOIS A. ET AL.: "In vitro evolution of an RNA-cleaving DNA enzyme into an RNA ligase switches the selectivity from 3'-5' to 2'-5'", J. AM. CHEM. SOC., vol. 125, 2003, pages 5346 - 5350, XP002985865 *
KUWABARA T. ET AL.: "A novel allosterically trans-activated ribozyme, the masizyme, with exceptional specificity in vitro and in vivo", MOLECULAR CELL, vol. 2, 1998, pages 617 - 627, XP000857827 *

Also Published As

Publication number Publication date
JP2007524395A (ja) 2007-08-30
EP1644531A2 (fr) 2006-04-12
EP1644531A4 (fr) 2009-03-11
WO2005001063A2 (fr) 2005-01-06
US20070105108A1 (en) 2007-05-10

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