WO2004108906A2 - Supports bacteriens pour sequences nucleotidiques codant des substances actives - Google Patents

Supports bacteriens pour sequences nucleotidiques codant des substances actives Download PDF

Info

Publication number
WO2004108906A2
WO2004108906A2 PCT/DE2004/001180 DE2004001180W WO2004108906A2 WO 2004108906 A2 WO2004108906 A2 WO 2004108906A2 DE 2004001180 W DE2004001180 W DE 2004001180W WO 2004108906 A2 WO2004108906 A2 WO 2004108906A2
Authority
WO
WIPO (PCT)
Prior art keywords
bacteria
cell
cells
gene
microorganism
Prior art date
Application number
PCT/DE2004/001180
Other languages
German (de)
English (en)
Other versions
WO2004108906A3 (fr
Inventor
Werner Goebel
Sabine Pilgrim
Jochen Strizker
Ivaylo Gentschev
Christoph Schoen
Original Assignee
Zentaris Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zentaris Gmbh filed Critical Zentaris Gmbh
Priority to DE112004001496T priority Critical patent/DE112004001496D2/de
Publication of WO2004108906A2 publication Critical patent/WO2004108906A2/fr
Publication of WO2004108906A3 publication Critical patent/WO2004108906A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne un micro-organisme bactérien comportant une séquence d'acide nucléique étrangère qui code une substance active. Selon l'invention, dans l'ADN chromosomique de ce micro-organisme, une séquence d'acide nucléique naturelle de la bactérie, qui code une enzyme bactérienne subit une délétion ou une mutation à condition que le produit de traduction résultant ne soit pas fonctionnel. Cette invention se rapporte en outre à des cellules qui sont infectées par lesdits micro-organismes, à des procédés de production de ces organismes, ainsi qu'à l'utilisation desdits micro-organismes, notamment pour produire des compositions pharmaceutiques.
PCT/DE2004/001180 2003-06-06 2004-06-07 Supports bacteriens pour sequences nucleotidiques codant des substances actives WO2004108906A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE112004001496T DE112004001496D2 (de) 2003-06-06 2004-06-07 Bakterielle Träger für Nukleotidsequenzen kodierend für Wirkstoffe

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10326189A DE10326189A1 (de) 2003-06-06 2003-06-06 Bakterielle Träger für Nukleotidsequenzen kodierend für Wirkstoffe
DE10326189.3 2003-06-06

Publications (2)

Publication Number Publication Date
WO2004108906A2 true WO2004108906A2 (fr) 2004-12-16
WO2004108906A3 WO2004108906A3 (fr) 2005-02-10

Family

ID=33494938

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2004/001180 WO2004108906A2 (fr) 2003-06-06 2004-06-07 Supports bacteriens pour sequences nucleotidiques codant des substances actives

Country Status (2)

Country Link
DE (2) DE10326189A1 (fr)
WO (1) WO2004108906A2 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998048026A1 (fr) * 1997-04-18 1998-10-29 Gesellschaft für Biotechnologische Forschung mbH Souche attenuee de salmonella utilisee en tant que vehicule d'immunisation orale
US5877159A (en) * 1995-05-03 1999-03-02 University Of Maryland At Baltimore Method for introducing and expressing genes in animal cells and live invasive bacterial vectors for use in the same
WO1999034007A1 (fr) * 1997-12-29 1999-07-08 Schering Aktiengesellschaft Introduction d'adn plasmidique codant pour un polypeptide dans le cytosol de macrophages par des bacteries suicidaires attenuees

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5877159A (en) * 1995-05-03 1999-03-02 University Of Maryland At Baltimore Method for introducing and expressing genes in animal cells and live invasive bacterial vectors for use in the same
WO1998048026A1 (fr) * 1997-04-18 1998-10-29 Gesellschaft für Biotechnologische Forschung mbH Souche attenuee de salmonella utilisee en tant que vehicule d'immunisation orale
WO1999034007A1 (fr) * 1997-12-29 1999-07-08 Schering Aktiengesellschaft Introduction d'adn plasmidique codant pour un polypeptide dans le cytosol de macrophages par des bacteries suicidaires attenuees

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
THOMPSON R J ET AL: "Pathogenicity and Immunogenicity of a Listeria monocytogenes Strain That Requires D-Alanine for Growth" INFECTION AND IMMUNITY, AMERICAN SOCIETY FOR MICROBIOLOGY. WASHINGTON, US, Bd. 66, Nr. 8, 1998, Seiten 3552-3561, XP002170925 ISSN: 0019-9567 *

Also Published As

Publication number Publication date
DE112004001496D2 (de) 2006-04-20
DE10326189A1 (de) 2005-01-05
WO2004108906A3 (fr) 2005-02-10

Similar Documents

Publication Publication Date Title
AU679436B2 (en) Stem cell inhibiting proteins
KR102276373B1 (ko) 유전자 치료 DNA 벡터 VTvaf17, 생산 방법； 대장균 균주 SCS110-AF, 생산 방법； 유전자 치료 DNA 벡터 VTvaf17을 가진 대장균 균주 SCS110-AF/VTvaf17, 생산 방법
DE69629576T2 (de) Verfahren zur herstellung von rekombinanten plasmiden
DE3308215C2 (de) Expression, Processing und Sekretion von heterologem Protein durch Hefe
AU2014256457B2 (en) Salmonella-based vectors for cancer immunotherapy targeting wilms' tumor gene WT1
KR101850162B1 (ko) 형질전환용 플라스미드
KR20070004585A (ko) 2-미크론 패밀리 플라스미드 및 이의 용도
DD209476A5 (de) Verfahren zur stabilisierung und selektierung von wirtszellen, die rekombinierende dna enthalten
CA2231784C (fr) Stabilisation de plasmides
DE3308030A1 (de) Tierische interferone
KR20170132784A (ko) 글로빈 유전자 클러스터의 조절 요소를 포함하는 진핵생물 발현 벡터
DE60204317T2 (de) Methode zur plasmidstabilisierung durch in vivo deletion der antibiotikaresistenz und selektion mit einem essentiellen gen
EP0775203B1 (fr) Procede de therapie genique utilisant des vecteurs d'adn sans gene marqueur de selection
WO2004108906A2 (fr) Supports bacteriens pour sequences nucleotidiques codant des substances actives
CN115518148A (zh) 手足口病免疫原性组合物、手足口病疫苗以及该疫苗的制备方法
EP1404808B1 (fr) Preparations fantomes exemptes d'acides nucleiques
KR20230079228A (ko) 람노스 대사 장애를 갖는 숙주 세포에서 재조합 단백질을 제조하는 방법 및 그의 발현 벡터, 숙주 세포 및 재조합 단백질
CN113355352B (zh) 一种以太子参TuMV-phe病毒基因为基础修饰改造病毒表达载体的方法
KR20220041928A (ko) 바실러스 리체니포르미스에서 단백질 생산을 증가시키기 위한 조성물 및 방법
EP0300425A2 (fr) Procédé de production de protéines sous forme soluble
JPH06508983A (ja) ライブラリースクリーニング法
CN113373101B (zh) 一种表面展示rgnnv mcp蛋白的重组芽孢及其制备方法与应用
DE19749973C1 (de) Lysozym-analoge Proteine und Peptide mit antimikrobieller Wirkung, ihre Herstellung und ihre Verwendung
KR20200010901A (ko) 프로모터 영역을 포함하는 분리된 폴리뉴클레오티드, 그를 포함하는 숙주 세포 및 상기 숙주 세포를 이용하여 목적 유전자를 발현시키는 방법
EP1000167B2 (fr) Production de proteines mutantes humaines dans des cellules humaines par recombinaison homologue

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 1120040014964

Country of ref document: DE

REF Corresponds to

Ref document number: 112004001496

Country of ref document: DE

Date of ref document: 20060420

Kind code of ref document: P

WWE Wipo information: entry into national phase

Ref document number: 112004001496

Country of ref document: DE

122 Ep: pct application non-entry in european phase