WO2004086979A1 - Swab for collecting biological specimens - Google Patents
Swab for collecting biological specimens Download PDFInfo
- Publication number
- WO2004086979A1 WO2004086979A1 PCT/EP2004/003392 EP2004003392W WO2004086979A1 WO 2004086979 A1 WO2004086979 A1 WO 2004086979A1 EP 2004003392 W EP2004003392 W EP 2004003392W WO 2004086979 A1 WO2004086979 A1 WO 2004086979A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- swab
- fibre
- tip
- rod
- layer
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/0045—Devices for taking samples of body liquids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F13/15—Absorbent pads, e.g. sanitary towels, swabs or tampons for external or internal application to the body; Supporting or fastening means therefor; Tampon applicators
- A61F13/38—Swabs having a stick-type handle, e.g. cotton tips
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5029—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures using swabs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/08—Ergonomic or safety aspects of handling devices
- B01L2200/087—Ergonomic aspects
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
Definitions
- the present invention relates to a swab for collecting biological specimens.
- swabs for collecting biological specimens of organic material consisting essentially of a cylindrical rod around one end of which, known as the tip, is wrapped a wad of fibre such as rayon or a natural fibre such as cotton, with hydrophilic properties to allow rapid absorption of the quantity of specimen to be collected and tested. Stable adherence of the fibre wrapped around the tip of the rod is generally achieved by gluing.
- a swab is immersed in a test-tube containing culture medium immediately after collection for appropriate conservation of the specimen during storage and /or transport thereof to the analytical laboratory.
- the tip of the cylindrical rod generally manufactured from essentially rigid material such as plastic, for example by extrusion, commonly presents a truncating cut which would make it difficult to insert the swab rod into the cavities (oral, nasal, ocular or rectal, urethral, vaginal etc.) of the patient from whom the specimen is taken, if the tip is not adequately protected. Therefore, the wad of hydrophilic fibre wrapped around said truncated end must not only contain sufficient material to allow absorption of the specimen in the desired quantity, in general 100 microlitres, but must also have a sufficiently thick and rounded shape to sheathe the edge of the truncated end so that it cannot cause damage or irritation to the patient during specimen collection.
- the fibre wad is wrapped around the tip of the rod in a rounded shape, typically developing into an ogive or similar shape so that it gradually becomes thicker towards the end of the rod thus reaching maximum thickness and therefore maximum protective effect, precisely around the truncated end.
- a wad of such a shape while protecting the patient from any risk of contact with said truncated end of the rod, results in a number of drawbacks.
- the main one is that the thickness of the wad, because of the hydrophilic nature of the fibre, leads to penetration of collected liquid specimen into the mass of said wad.
- the sample is released from the swab at the moment of analysis by simply gripping the rod of the swab and delicately sliding its tip and hence the fibre impregnated with liquid, along for example a petri dish with culture medium, in practice by spreading the specimen onto this latter (swabbing), even if this operation is repeated and is careful, it does not enable the entire volume e.g. the 100 ml of absorbed specimen to be released, because that part of it which has penetrated into the interior of the wad in the direction of its tip cannot be pressed out towards the surface and hence released by the swab during this operation
- the present invention proposes a swab for collecting biological specimens of the type consisting of a rod terminating with a tip covered in fibre with hydrophilic properties to allow absorption of said specimens, characterised in that said fibre covers said tip in the form of a layer applied by means of flocking.
- Said example refers to the case of a swab suitable for both the collection and storage of a biological specimen, and therefore also includes a test-tube containing a culture medium suitable for the collected microorganisms into which the swab is to be immersed after collection, such as for example the type described in the aforementioned patent EP0643131 by the same Applicant.
- Figure 1 shows an exploded view of the two components of a device in accordance with the example, that is the swab and test-tube, whereby the test- tube is partially sectioned longitudinally.
- FIG. 2 shows an enlarged detail of the swab of Figure 1 in section.
- a device of the invention in accordance with the illustrated example comprises an essentially cylindrical test-tube 10 containing a culture medium in gel form 11, presenting a free surface level 12 inside the test- tube.
- the upper open end of the test-tube presents a collar 13 for receiving a closure means.
- the device is completed by a swab 20 consisting of a rod 14 carrying at one end a stopper 15 which has to act as the closure means of the test-tube and is hence shaped so that it can engage, for example by snap-engaging, with the collar 13 of the test-tube.
- the rod 14 terminates with a tip 16 carrying a suitable means, for example a layer of fibre 17, for collecting the specimen to be analysed.
- said tip 16 of the rod is shaped in a rounded geometry, similar to an ogive, and said fibre 17 being disposed as a layer of uniform thickness.
- said fibre with hydrophilic properties is deposited by means of flocking.
- the flocking technique is preferably of the type conducted in an electrostatic field which deposits the fibres in an ordered manner, perpendicular to the surface of the tip of the swab rod, which has been previously coated with adhesive for example by immersion or spraying.
- the fibre which is to form the flocked layer is subjected to an electrostatic field, and is hence deposited in an oriented manner and anchored to the surface of the tip, being retained by the adhesive.
- the adhesive is preferably water-based: once dried it enables the fibre to be anchored in a stable manner to the swab and to resist abrasion.
- the flocked swab is then dried by exposing it to a source of heat or radio- frequency.
- the tip of the swab stem is covered with a layer of fibre, preferably of uniform thickness, and from 0.6 to 3 mm thick.
- the fibre count, i.e. the weight in grams per 100 linear metres of a single fibre, is preferably between 1.7 and 3.3 Dtex.
- a fibre of 0.6 mm length and 1.7 Dtex can be applied by flocking to obtain a fine nap
- a fibre up to 3 mm in length and 3.3 Dtex can be applied to obtain a long nap, obtaining, for values intermediate between the aforedefined, corresponding intermediate characteristics of thickness and fineness of the flocked layer.
- the expedient to be respected according to the objects of the invention is to maintain an ordered arrangement of the fibres, substantially parallel to each other and normal to the surface of the rod, avoiding any overlapping of fibres which can occur if the nap is too long. Indeed, in this manner the capillary represented by each fibre, by virtue of which it can carry out its task of absorbing and releasing essentially the same quantity of specimen, remains unimpaired and functional.
- the amount of fibre to be deposited for forming the flocked layer in accordance with the invention is determined on the basis of the type of fibre and the pre- chosen layer characteristics of thickness and fineness, in such a manner as to enable 100 microlitres of specimen to be absorbed.
- the fibre is chosen from a wide range of materials provided they are hydrophilic by capillarity, such as for example, synthetic or artificial materials e.g. rayon, polyester, polyamide, carbon fibre or alginate, natural materials e.g. cotton and silk, or mixtures thereof.
- synthetic or artificial materials e.g. rayon, polyester, polyamide, carbon fibre or alginate, natural materials e.g. cotton and silk, or mixtures thereof.
- a swab is prepared using a plastic rod, suitable for human clinical collection, of diameter 2.5 mm which decreases to 1 mm over a length of about 6 cm.
- the tip of the part with the smallest diameter is dipped in or sprayed with an adhesive, then the rod is placed vertically in a flocking apparatus in electrostatic field to deposit a polyamide flock.
- the polyamide flock of 0.7 mm length and 1.7 Dtex allows 0.5 ⁇ l per mm 2 to be absorbed, therefore by flocking the 10 mm long tip of said rod the absorbing capacity obtained is 40 ⁇ l.
- a rod with a spatulate end is used, suited for example to collecting organic specimens from the oral cavity of a patient.
- Polyester fibre of 1 mm length and 1.7 Dtex count are used for the flocking.
- polyester fibre of 2 mm length and 2.5 Dtex count is used.
- a swab of the invention is capable of releasing about 90% of the absorbed specimen by swabbing, in this manner considerably increasing the sensitivity of the analysis compared with swabs of the known art, in particular by almost completely eliminating the risk of false negatives resulting from the incomplete release of the collected specimen from swab to test plate.
- a fibre layer of any thickness, even very small, around the tip of the rod rather than a mass to cover it, as in the known art means that the required rounded shape of the swab, i.e.
- a swab can be provided with a rounded tip by virtue of its shaping, around which a thin layer of fibre is deposited by flocking to allow on the one hand collection of a sufficient quantity of specimen for analysis, and on the other to minimize the total bulk of the part of the swab which is to penetrate the urethra, in consequence so reducing the discomfort of the patient undergoing the collection procedure.
- the shape given to the tip of the swab nevertheless varies greatly according to the type of collection it is intended for, and can even be truncated or have edges when the type of collection (for example oral) allows it.
- the type of adhesive, type of fibre and fibre characteristics, such as length and count are in any case chosen from a wide range of options in order to obtain an ideal specific marker for identifying the microbiological specimen, whether by a direct diagnostic technique, by immuno- test, or by molecular biology techniques such as PCR, or with other known culturing, enrichment or selection techniques.
- the specimen to be collected with a swab of the invention generally consists of bacteria or viruses or DNA or RNA or a mixture thereof.
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Heart & Thoracic Surgery (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Epidemiology (AREA)
- Vascular Medicine (AREA)
- Surgery (AREA)
- Pathology (AREA)
- Medical Informatics (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Claims
Priority Applications (18)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2004226798A AU2004226798B2 (en) | 2003-04-01 | 2004-03-31 | Swab for collecting biological specimens |
CA002515205A CA2515205C (en) | 2003-04-01 | 2004-03-31 | Swab for collecting biological specimens |
JP2006504927A JP4579902B2 (en) | 2003-04-01 | 2004-03-31 | Swab for collecting biological specimens |
EP04724556.8A EP1608268B2 (en) | 2003-04-01 | 2004-03-31 | Swab for collecting biological specimens |
DK04724556.8T DK1608268T4 (en) | 2003-04-01 | 2004-03-31 | Swab for taking biological samples |
ES04724556T ES2297406T5 (en) | 2003-04-01 | 2004-03-31 | Swab to collect biological samples |
US10/543,873 US8114027B2 (en) | 2003-04-01 | 2004-03-31 | Swab for collecting biological specimens |
DE202004021787U DE202004021787U1 (en) | 2003-04-01 | 2004-03-31 | Swabs for receiving biological samples |
NZ541560A NZ541560A (en) | 2003-04-01 | 2004-03-31 | Swab for collecting biological specimens |
DE602004010240.7T DE602004010240T3 (en) | 2003-04-01 | 2004-03-31 | DUTCH FOR RECEIVING BIOLOGICAL SAMPLES |
US12/903,921 US8317728B2 (en) | 2003-04-01 | 2010-10-13 | Method of using flocked swab for collecting biological specimens |
US13/361,584 US8979784B2 (en) | 2003-04-01 | 2012-01-30 | Swab for collecting biological specimens |
US13/531,800 US9011358B2 (en) | 2003-04-01 | 2012-06-25 | Swab for collecting biological specimens |
US13/657,949 US11364018B2 (en) | 2003-04-01 | 2012-10-23 | Swab for collecting biological specimens |
US13/899,394 US9173779B2 (en) | 2003-04-01 | 2013-05-21 | Swab for collecting biological specimens |
US14/858,792 US10327741B2 (en) | 2003-04-01 | 2015-09-18 | Swab for collecting biological specimens |
US16/448,398 US20190307433A1 (en) | 2003-04-01 | 2019-06-21 | Swab for Collecting Biological Specimens |
US17/744,421 US11446012B2 (en) | 2003-04-01 | 2022-05-13 | Swab for collecting biological specimens |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IT000643A ITMI20030643A1 (en) | 2003-04-01 | 2003-04-01 | BUFFER FOR THE COLLECTION OF BIOLOGICAL SAMPLES |
ITMI2003A000643 | 2003-04-01 |
Related Child Applications (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US10543873 A-371-Of-International | 2004-03-31 | ||
US10/543,873 A-371-Of-International US8114027B2 (en) | 2003-04-01 | 2004-03-31 | Swab for collecting biological specimens |
US12/903,921 Continuation US8317728B2 (en) | 2003-04-01 | 2010-10-13 | Method of using flocked swab for collecting biological specimens |
US13/361,584 Continuation US8979784B2 (en) | 2003-04-01 | 2012-01-30 | Swab for collecting biological specimens |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2004086979A1 true WO2004086979A1 (en) | 2004-10-14 |
Family
ID=33105040
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2004/003392 WO2004086979A1 (en) | 2003-04-01 | 2004-03-31 | Swab for collecting biological specimens |
Country Status (10)
Country | Link |
---|---|
US (9) | US8114027B2 (en) |
EP (1) | EP1608268B2 (en) |
JP (1) | JP4579902B2 (en) |
CA (1) | CA2515205C (en) |
DE (6) | DE202004021930U1 (en) |
DK (1) | DK1608268T4 (en) |
ES (1) | ES2297406T5 (en) |
IT (1) | ITMI20030643A1 (en) |
NZ (1) | NZ541560A (en) |
WO (1) | WO2004086979A1 (en) |
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US7897365B2 (en) | 2003-11-18 | 2011-03-01 | Charm Sciences, Inc. | Method for adjusting antibiotic sensitivity of a test culture |
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