WO2004063720A1 - Collecte de materiau cible - Google Patents

Collecte de materiau cible Download PDF

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Publication number
WO2004063720A1
WO2004063720A1 PCT/AU2004/000057 AU2004000057W WO2004063720A1 WO 2004063720 A1 WO2004063720 A1 WO 2004063720A1 AU 2004000057 W AU2004000057 W AU 2004000057W WO 2004063720 A1 WO2004063720 A1 WO 2004063720A1
Authority
WO
WIPO (PCT)
Prior art keywords
opening
closure
collection
sample
target material
Prior art date
Application number
PCT/AU2004/000057
Other languages
English (en)
Inventor
David Ian Berryman
Original Assignee
Murdoch University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Murdoch University filed Critical Murdoch University
Priority to AU2004204144A priority Critical patent/AU2004204144A1/en
Publication of WO2004063720A1 publication Critical patent/WO2004063720A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G01N1/08—Devices for withdrawing samples in the solid state, e.g. by cutting involving an extracting tool, e.g. core bit

Definitions

  • This invention relates to collection of target material.
  • the invention relates to a device for collecting target material, and to collection apparatus incorporating such a collection device.
  • the target material may be in any appropriate form, such as a sample taken from source material, or a discrete item.
  • the invention has been devised particularly, although not solely, for field collection of sample material, such as plant sample material, from which genetic analysis can later be conducted.
  • apparatus for collection of target material comprising a body defining a collection chamber having an opening to receive target material therein, and applicator means for applying a closure to the opening after reception of the target material in the collection chamber.
  • the body comprises a plurality of collection chambers.
  • guide means are provided for guiding movement of the applicator means from one collection chamber to the next chamber.
  • An indexing mechanism is preferably provided whereby the applicator means is brought into registration with the next successive collection chamber after completing insertion of the closure in one collection chamber.
  • the closure comprises a stopper.
  • other appropriate forms of closure including caps and adhesive coverings such as films and foils.
  • the closure and the periphery of the opening co-operate to cause separation of the sample from the source material upon application of the closure to the opening.
  • the co-operation between the closure and the periphery of the opening to the collection chamber involves interaction to provide a cutting action for cutting a sample from source material to provide the target material.
  • the source material is placed adjacent the chamber opening with the closure approaching the collection chamber from the opposed side of the source material thereby to cut a sample from the source material and carry the cut sample into the collection chamber.
  • Either the closure or the periphery of the opening may be configured to present a cutting or weakening edge to facilitate separation of the sample from the source material.
  • the body has a surface onto which each collection chamber opens at said opening therein, the surface providing a locating surface against which the source material is located in preparation for a sample to be cut therefrom upon contact with the closure during application thereof to the opening.
  • the body comprises a well plate of known kind.
  • the closures are fed successively to a station within the applicator means. This can be achieved in appropriate way, such as by having the closures provided in a magazine or on a feeder strip.
  • a collection device comprising an attachment means for releasable attachment to a body defining a collection chamber having an opening to receive target material therein, and an applicator means for applying a closure to the opening after reception of target material in the collection chamber.
  • the applicator means is adapted to apply the closure to the opening in such manner that interaction between the closure and the opening causes separation of target material from source material positioned therebetween and pushes said target material into the opening during application of the closure to the opening.
  • the collection device may further comprise an indexing mechanism whereby the applicator means is caused to move successively into registration with each collection chamber.
  • an apparatus for collection of sample material comprising a body defining a collection chamber having an opening, a closure for closing the opening, and means for applying the closure into the opening, the closure and the opening cooperating to cause separation of sample material from source material positioned between the closure and the opening as the closure is applied to the opening.
  • a collection device adapted to cooperate with a well plate to provide sample collection apparatus according to the first or third aspects of the invention.
  • a sample collection device adapted to cooperate with a body defining a collection chamber having an opening to receive sample material therein, the sample collection device comprising applicator means for applying a closure to the opening whereby interaction between the closure and the opening causes separation of a sample from source material positioned therebetween and pushes said sample material into the opening during application of the closure to the opening.
  • the sample collection device further comprises means for detachably connecting the applicator means to the body.
  • the body defines a plurality of collection chambers.
  • the body is a conventional well plate.
  • the sample collection device may further comprise an indexing mechanism whereby the applicator means is caused to move successively into registration with each collection chamber.
  • Figure 1 is a perspective view of a sample collection device according to a first embodiment, fitted onto a well plate to provide a sample collection apparatus;
  • Figure 2 is a schematic view illustrating operational parts of sample collection device
  • Figure 3 is a plan view of the well plate
  • Figure 4 is a sectional view of the well plate of Figure 3;
  • Figure 5 is a schematic view illustrating the indexing path followed by an applicator means with respect to the well plate
  • Figure 6 is a schematic illustrating the sample collection apparatus in operation, with a sample about to be cut from target material
  • Figure 7 is of view similar to Figure 6 with the exception that the sample has been cut and the collection well closed by way of a stopper;
  • Figure 8 is a schematic view illustrating the sample collection apparatus in operation using an alternative form of well plate, with a sample about to be cut from target material;
  • Figure 9 is a view similar to Figure 8 with the exception that the sample has been cut and the collected well closed by way of a stopper;
  • Figure 10 is a perspective view of a sample collection device according to a second embodiment, fitted onto a well plate to provide a sample collection apparatus;
  • Figure 11 is a schematic elevational view of a magazine which forms part of the apparatus of Figure 10 for storage of batches of stoppers;
  • Figure 12 is a schematic plan view of part of the magazine. Best Mode(s) for Carrying Out the Invention
  • the first embodiment which is shown in Figures 1 to 7, is directed to a sample collection device 10 for field collection of samples taken from source material such as plant leaves including in particular wheat leaves.
  • the sample collection device 10 is used in association with a conventional well plate 11 comprising a body 13 having an upper face 15 and a plurality of wells 17 each having an opening 19 which opens on to the upper face 15. Each opening 19 has a peripheral edge 20. Thus, each collection well 17 defines a collection chamber.
  • the sample collection device 10 and the well plate 11 in combination provide a sample collection apparatus.
  • the sample collection device 10 comprises an applicator means 21 , an attachment means 23 for releasably attaching the device 10 to the well plate 11 , and an indexing mechanism 25 for indexing movement of the applicator means 21 with respect to the attachment means 23 and thereby with respect to the well plate 11 connected thereto, as will be explained in more detail later.
  • the applicator means 21 is adapted to insert closures 31 sequentially into the wells 17 through the openings 19 thereof.
  • Each closure 31 comprises a stopper such as a cap having a body 33 with a cylindrical sidewall 35, a bottom wall 36 and a top wall 37.
  • a cavity 38 extends in to the body 33 through the top wall 37, as best seen in Figures 6 and 7 of the drawings.
  • the body 33 defines a peripheral edge 39 at the junction between the sidewall 35 and the bottom wall 36.
  • the applicator means 21 comprises a body 41 having a portion thereof configured as a handle 43.
  • An operating lever 45 is pivotally mounted on the body 41 at pivot 47, with a portion of the lever 45 adjacent one end thereof being located adjacent the handle 43, thereby to provide a trigger 49 operable by a user gripping the handle 43.
  • the other end of the operating leaver 45 is operatively connected to a plunger 51 by way of a pivot 53.
  • the plunger 51 is guided for sliding movement within the body 21 in response to operation of the trigger 49.
  • the body 41 incorporates a station 55 into and out of which the plunger 51 can move in a reciprocating fashion in response to operation of the trigger 49.
  • a feeder mechanism (not shown) is provided for moving stoppers 31 sequentially in to the station 55 from where the particular stopper occupying the station can be inserted into a respective well 17 in registration therewith under the action of the plunger.
  • the plunger 51 operates by entering the aperture 38 within the stopper 31 , and then pushing the stopper out of the station 55 towards the well 17 in registration with the station and into that well through the opening 19 therein.
  • the stopper 31 In addition to serving to close the well 17, the stopper 31 also cooperates with the well to perform a cutting action for cutting a sample from target material and conveying the sample in to the well.
  • This is best seen with reference to Figures 6 and 7 of the drawings, which show target material in the form of a leaf 61 positioned against the upper face 15 of the well plate 11.
  • the target material 61 is disposed between the well plate 11 and the station 55 such that the particular well to receive the cut sample is disposed to one side of the target material and the particular stopper 31 for closing that well 17 is disposed to the other side of the target material.
  • the applicator means 21 is automatically indexed to the next well 17 within the well plate 11 in readiness to collect the next sample.
  • the indexing action occurs on the return movement of the operating lever 45 following withdrawal of the plunger 51 from the stopper 31 in the well just sealed.
  • the path of indexing movement is illustrated schematically in Figure 5 of the drawings and identified by reference numeral 70. From the drawing, it can be seen that the path 70 has longitudinal sections 71 and lateral sections 72.
  • the attachment means 23 includes a clamping mechanism 24 for clampingly engaging the well plate 11 at the periphery thereof.
  • the indexing mechanism 25 includes guide rails 73, 74 on which the applicator means 21 is mounted for movement corresponding to the longitudinal direction of the indexing path 70.
  • the guide rail 73 incorporates detents 75, each of which correspond to one of the wells 17 in each longitudinal section 71 of the indexing path 70.
  • the applicator means 21 indexes along the longitudinal section 71 of the indexing path 70 from one detent 75 to the next following each operation of the applicator means 21 to install a stopper 31.
  • the indexing mechanism 25 further includes means 77 for advancing the applicator means 21 through a lateral section 72 of the indexing path 70 to the next longitudinal section 71.
  • the means 77 includes a control knob 79 which is rotatable by a operator for advancing the applicator means 21 from one longitudinal section 71 to the next longitudinal section 71 along the respective lateral section 72 therebetween.
  • the identity of samples collected can be recorded manually, or alternatively the collection device 10 may be equipped with means for recording purposes.
  • the collection device 10 may incorporate a microprocessor or computer to establish electronic records of sample location. This may involve utilisation of a camera capable of reading digital codes on samples and associating each sample code with the location of the collection well 17 in which a particular sample is retained.
  • voice recording may also be provision for voice recording to allow spoken notes to be linked to each individual sample. This may be beneficial, for example, when samples are grown in an alpha-numeric array, matching the well plate 11 , in which some individual samples are missing from the array.
  • sample numbers could be linked by bar coding, which could be included on the closure for each collection well, or a code on the original sample source could be linked by appropriate software to a particular location within the well plate.
  • the present embodiment provides a simple yet highly effective arrangement for automating the process of sample collection to some extent.
  • the embodiment provides a way of performing a cutting action on target material such as a plant leaf, delivering a sample cut therefrom into one of the collection wells 17, sealing the respective collection well 17 by way of a stopper 31 and thereafter indexing the applicator means 21 to the next collection well 17 in readiness to receive another piece of target material from which a sample is to be obtained.
  • each collection well 17 was open by way of opening 19 until such time as it was closed by application of closure 31.
  • Such an arrangement is illustrated in Figures 8 and 9, where each collection well 17 of the well plate 11 is provided with a frangible seal defined by a membrane 81 positioned across the opening 19.
  • the membrane 81 can seal the collection well 17 against ingress of any contaminant material.
  • the membrane 81 can retain matter 83 already present in the collection well 17.
  • the retained matter 83 maybe of any appropriate form.
  • the retained matter 83 comprises a liquid 85 for performing a preliminary process on the sample to be subsequently introduced, as well as a homogenisation ball 87.
  • the membrane 81 is fractured by being cut, along with the sample 65, by interaction between the closure 31 and the periphery 20 of the opening 19, during application of the closure 31.
  • the cut portion 82 of the membrane 81 deposits in the collection chamber but does not adversely interfere with the sample therein.
  • FIG. 10 to 12 there is shown a collection device according to a second embodiment.
  • the collection device 90 according to the second embodiment is similar to the collection device 10 according to the first embodiment and corresponding reference numerals are utilised to identify corresponding parts.
  • the device 90 incorporates a magazine structure 91 for accommodating a multitude of closures 31 in the form of stoppers.
  • the magazine 91 comprises a housing 93 which is rotatably mounted on shaft 94 and which incorporates a plurality of elongate storage chambers 95 in circumferentially spaced relation. Each storage chamber 95 is adapted to receive a batch of stoppers 31 positioned in series one upon another.
  • the housing 93 is positioned above a moveable base 97 which incorporates a positioning hole 99.
  • the base 97 is moveable in order to convey the positioning hole 99 between a receiving station to a discharge station.
  • the housing 93 is rotateable so that each of the storage chambers 95 can be moved selectively into registration with the positioning hole 99 when the latter is at the receiving station.
  • Stoppers 31 in the particular storage chamber 95 in registration with the positioning hole 99 are sequentially moved into the positioning hole.
  • the base 97 is then moved to convey the particular stopper 31 accommodated within the positioning hole 99 from the receiving station to the discharge station, at which the stopper concerned can be inserted into a respective collection well 17 in registration therewith under the action of the plunger 51 of the applicator means 21.
  • the stoppers 31 in the particular storage chamber 95 in registration with the positioning hole 99 when the latter is at the receiving station, are urged towards the positioning hole under the influence of a spring-loaded plunger 103.
  • the plunger 103 is withdrawn from that chamber concerned and the housing 93 indexed so as to move the next chamber into registration with the positioning hole 99 at the receiving station.
  • the plunger 103 is then inserted into the chamber 95 in registration with the positioning hole 99 so as to urge the batch of stoppers 31 therein towards the positioning hole.
  • the invention need not be limited to the particular embodiments described.
  • the embodiments have been described in relation to collection of samples from plant material (such as leaves of wheat).
  • the invention may be utilized for the collection of any appropriate material including for example animal material.
  • the invention may for example be employed to collect specimens from the ears of animals by cutting a piece therefrom.
  • the invention may also be used for collection of target material other than samples taken from source material such as leaves.
  • the invention may be applied to the collection of discrete items, such as seeds, invertebrate animals, and inorganic substances. With the collection of discrete items, it is obviously unnecessary to use the feature whereby the closure can cut target material from source material.
  • the collection device When used for collection of discrete items, the collection device may be equipped with a transfer mechanism, such as a funnel, through which samples can be conveyed to the collection chamber, with the opening in the collection chamber being subsequently closed by application of a closure in a manner somewhat similar to that previously described.
  • a transfer mechanism such as a funnel
  • the applicator means 21 was manually operable by way of the operating lever 45. Operation of the lever 45 actuates the plunger 51 and also the indexing mechanism 25, as described.
  • Other arrangements are, of course, possible, including an automated process.
  • a solenoid mechanism may be provided for activating the plunger 51 and electronic "stepper" motors may be employed for effecting relative indexing movement between the applicator means 21 and the well plate 11.
  • the device 10 is adapted for use with conventional well plates which are disposable after use. It should be appreciated that the invention is not limited to use with conventional well plates.
  • dedicated collection chambers can be provided for use with the collection device if so desired.
  • the invention may also be provided with an arrangement for selectively masking each of the collection chambers other than the particular collection chambers in use at any particular time. Such an arrangement may be advantageous in assisting to avoid cross contamination between the various collection chambers.

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

La présente invention concerne un appareil s'utilisant pour recueillir un matériau cible tel qu'un échantillon prélevé sur le matériau source (61), par exemple, une feuille. L'appareil comporte un corps ainsi qu'un plateau à cupules (11) définissant une chambre de collecte (17) comportant une ouverture (19) pour y recevoir le matériau échantillon. L'appareil comporte également un applicateur (21) à piston (51) permettant d'appliquer sur l'ouverture (19) une occultation telle qu'un bouchon (31) après réception de l'échantillon de matériau dans la chambre de collecte (17). Lorsque l'appareil sert à recueillir du matériau cible sous forme d'un échantillon prélevé dans le matériau source, le bouchon (31) et le pourtour (20) de l'ouverture (19) coopèrent de façon à découper l'échantillon dans le matériau source par application du bouchon (31) sur l'ouverture (19).
PCT/AU2004/000057 2003-01-16 2004-01-16 Collecte de materiau cible WO2004063720A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2004204144A AU2004204144A1 (en) 2003-01-16 2004-01-16 Collection of target material

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2003900192A AU2003900192A0 (en) 2003-01-16 2003-01-16 Sample collection apparatus
AU2003900192 2003-01-16

Publications (1)

Publication Number Publication Date
WO2004063720A1 true WO2004063720A1 (fr) 2004-07-29

Family

ID=30004899

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AU2004/000057 WO2004063720A1 (fr) 2003-01-16 2004-01-16 Collecte de materiau cible

Country Status (2)

Country Link
AU (1) AU2003900192A0 (fr)
WO (1) WO2004063720A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103439141A (zh) * 2013-08-27 2013-12-11 中国农业科学院棉花研究所 功能叶片取样机
WO2014053792A2 (fr) * 2012-10-05 2014-04-10 Vilmorin & Cie Outil de prelevement de materiel vegetal, automate le comportant, cellule de prelevement equipee d'un tel automate et procede de prelevement
CN103257054B (zh) * 2006-03-02 2016-07-06 孟山都技术有限公司 自动无污染种子取样器和对种子进行取样、检测和分群的方法
WO2016197122A1 (fr) * 2015-06-05 2016-12-08 Orion Genomics Llc Dispositif de collecte d'échantillons

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3921459A (en) * 1974-10-09 1975-11-25 Norman F Willett Precision leaf sampling device
EP0278677B1 (fr) * 1987-02-11 1992-07-22 Secretary Of State For Scotland Dispositif de prélèvement d'échantillons
US5510081A (en) * 1991-11-13 1996-04-23 Queensland University Of Technology Test sampling apparatus
US5587062A (en) * 1996-01-24 1996-12-24 Shimadzu Corporation Sample collecting apparatus by gel electrophoresis
US5638170A (en) * 1994-06-07 1997-06-10 Hudson Control Group, Inc. Punched carrier specimen sample processor

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3921459A (en) * 1974-10-09 1975-11-25 Norman F Willett Precision leaf sampling device
EP0278677B1 (fr) * 1987-02-11 1992-07-22 Secretary Of State For Scotland Dispositif de prélèvement d'échantillons
US5510081A (en) * 1991-11-13 1996-04-23 Queensland University Of Technology Test sampling apparatus
US5638170A (en) * 1994-06-07 1997-06-10 Hudson Control Group, Inc. Punched carrier specimen sample processor
US5587062A (en) * 1996-01-24 1996-12-24 Shimadzu Corporation Sample collecting apparatus by gel electrophoresis

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103257054B (zh) * 2006-03-02 2016-07-06 孟山都技术有限公司 自动无污染种子取样器和对种子进行取样、检测和分群的方法
WO2014053792A2 (fr) * 2012-10-05 2014-04-10 Vilmorin & Cie Outil de prelevement de materiel vegetal, automate le comportant, cellule de prelevement equipee d'un tel automate et procede de prelevement
FR2996641A1 (fr) * 2012-10-05 2014-04-11 Alci Outil de prelevement de materiel vegetal, automate le comportant, cellule de prelevement equipee d'un tel automate et procede de prelevement
WO2014053792A3 (fr) * 2012-10-05 2014-06-26 Vilmorin & Cie Outil de prelevement de materiel vegetal, automate le comportant, cellule de prelevement equipee d'un tel automate et procede de prelevement
US9250161B2 (en) 2012-10-05 2016-02-02 Vilmorin & Cie Tool for sampling plant material, automation comprising same, sampling cell provided with such an automation and sampling method
EP3168594A1 (fr) * 2012-10-05 2017-05-17 Vilmorin & Cie Outil de prélèvement de matériel végétal, automate le comportant, cellule de prélèvement équipée d'un tel automate et procédé de prélèvement
CN103439141A (zh) * 2013-08-27 2013-12-11 中国农业科学院棉花研究所 功能叶片取样机
WO2016197122A1 (fr) * 2015-06-05 2016-12-08 Orion Genomics Llc Dispositif de collecte d'échantillons
US10508975B2 (en) 2015-06-05 2019-12-17 Orion Genomics Llc Sample collection device
US11268885B2 (en) 2015-06-05 2022-03-08 Orion Genomics Llc Sample collection device

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