WO2004058210A1 - Composition cosmetique contenant un precurseur de melanine - Google Patents

Composition cosmetique contenant un precurseur de melanine Download PDF

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Publication number
WO2004058210A1
WO2004058210A1 PCT/EP2003/014513 EP0314513W WO2004058210A1 WO 2004058210 A1 WO2004058210 A1 WO 2004058210A1 EP 0314513 W EP0314513 W EP 0314513W WO 2004058210 A1 WO2004058210 A1 WO 2004058210A1
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WO
WIPO (PCT)
Prior art keywords
regulate
kit
inhibit
skin
enzyme
Prior art date
Application number
PCT/EP2003/014513
Other languages
English (en)
Inventor
Kalpana Kamalakar Nayak
Govindarajan Raman
Savitha Sharma
Sushama Shripad Wagh
Original Assignee
Unilever Plc
Unilever Nv
Hindustan Lever Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever Plc, Unilever Nv, Hindustan Lever Limited filed Critical Unilever Plc
Priority to AU2003290087A priority Critical patent/AU2003290087A1/en
Publication of WO2004058210A1 publication Critical patent/WO2004058210A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00—Preparations for care of the hair
    • A61Q5/006—Antidandruff preparations
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/3481—Organic compounds containing oxygen
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/3571—Microorganisms; Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
    • A61K8/22—Peroxides; Oxygen; Ozone
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/34—Alcohols
    • A61K8/347—Phenols
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q15/00—Anti-perspirants or body deodorants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005—Antimicrobial preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/70—Biological properties of the composition as a whole
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/74—Biological properties of particular ingredients
    • A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
    • A61K2800/782—Enzyme inhibitors; Enzyme antagonists
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/88—Two- or multipart kits

Definitions

  • the present invention relates to products adapted to serve as inhibitor/functional modifier of proteins, and in particular a kit/formulations useful for such inhibition/functional modification of proteins for cosmetic applications such as an anti-aging aid, anti-microbial applications, malodour inhibition as well as for application in food/beverage processing.
  • the invention also relates to a method to enhance preservative/storage stability of food stuffs, and the like.
  • the invention is also directed to method of cosmetic /personal care applications of the kit/formulation of the invention such as for improving/retaining the skin conditions, in anti- microbial applications and in food processing/preservation, and the like.
  • Functional proteins are those which exhibit properties like binding to ligands, barrier or receptor properties in membranes, catalytic properties, regulatory properties through their presence/absence could either be stimulatory/inhibitory, or serve to give integrity/shape, role which can be assayed. These also include proteins which can be physiologically converted to functional proteins. Functional proteins that exhibit catalytic activity are called enzymes.
  • Proteinases or peptidases are enzymes classified under hydrolases and are active in catalyzing the breakdown of peptide bonds, a process which is conventionally known as proteolysis .
  • proteinases play a significant role in altering physiological conditions which again affect cosmetic conditions such as skin glow/elasticity, scaly scalp, and the like. Moreover some proteinases are also responsible for enzymatic spoilage of food products.
  • Collagen a predominant skin matrix protein is known to impart tensile strength to skin. It is also known in the art that the levels of collagen in skin are significantly reduced with age and/or photoda aged skin. Many studies have shown that the levels of collagen type-I in skin are decreased with age and/or with increased photo-damage (for example Lavker, R. J. Inv. Derm. (1979, 1973, 1979-1966- Griffiths et al. N. Eng. J. Med. (1993) 329, 530-535. The lower level of collagen in skin is accordingly associated with decrease in the tensile strength of the skin causing wrinkles and laxity. This decrease is thought to be due to enzymatic breakdown of collagen and similar proteins in the skin.
  • Elastin is the protein that gives body tissues their elasticity. It is particularly important in skin wherein it imparts useful suppleness and resilience. The elastin fibers along with collagen help skin to maintain its tensile strength, as well as the ability to recoil after being stretched. It is therefore important that adequate levels of elastin are maintained. 3 -
  • Bacteria are ubiquitous in nature. They are present as normal flora in nearly all natural surfaces like skin, teeth, oral mucosa, in the gastro-intestinal tract etc. While they have a protective function as normal flora, they are responsible for several undesirable conditions like spoilage, production of malodor and diseases. Bacteria are able to metabolize sugars, proteins and carbohydrates to cause spoilage.
  • Bacteria are able to cause diseases of skin, such as e.g. pimples, boils etc. Bacteria also cause diseases of the gastro-intestinal tract like typhoid, gastro enteritis, cholera, dysentery and diarrhea. Bacteria in the axilla break down the secretions of the sebaceous and apocrine glands to produce volatile fatty acids, steroids and thiols that are responsible for body odor. Bacterial growth on the teeth called plaque in responsible for caries, and bacteria residing on the tongue cause oral malodor.
  • Antibacterial compounds are used for treatment of diseases.
  • Antimicrobial compounds are included in formulations like soaps, deos, floor cleaners and tooth pastes that are meant to control the growth of bacteria on body and other surfaces.
  • Perspiration or sweat is basically a clear liquid excreted by the sudoriparous glands. Sebum is a secretion of sebaceous glands. Sweat, sebum and such secretions in combination with skin bacteria, provide a suitable medium for the elaboration of malodor molecules from precursors present in sweat and sebum.
  • lipases are key enzymes responsible for spoilage in food products such as oily and dairy products. Inherent lipases present in the food products or unintended exogenous additions or due to their release by lipolytic microbes could breakdown the lipids present in the food product resulting in the spoilage of the product.
  • US 6,423,325 discloses a cosmetic composition adapted to serve as an anti-aging formulation comprising a specific composition of lipid components, in particular a first lipid selected from the petroselinic acid and derivatives thereof, and a second lipid selected from linoleic acid, conjugated linoleic acid and mixtures thereof in a dermatologically acceptable vehicle.
  • US 6,440,434 proposed a cosmetic composition for application to the skin comprising a specific fatty acid, namely petroselinic acid and/or derivatives thereof, in combination with a retinoid selected from the group consisting of retinoid acid, retinol and retinyl linoleate and a dermatologically acceptable vehicle to advantageously provide further skin benefits in addition to anti-aging such as soothing sensitive and/or irritated skin, controlling oil/sebum secretion and for lightening the skin.
  • a specific fatty acid namely petroselinic acid and/or derivatives thereof
  • a retinoid selected from the group consisting of retinoid acid, retinol and retinyl linoleate
  • a dermatologically acceptable vehicle to advantageously provide further skin benefits in addition to anti-aging such as soothing sensitive and/or irritated skin, controlling oil/sebum secretion and for lightening the skin.
  • US 6455057 discloses yet another cosmetic composition useful as an anti-aging substance for skin comprising a specific fatty acid, namely petroselinic acid and/or derivatives thereof, in combination with a phenolic compound and a dermatologically acceptable vehicle.
  • This prior art also suggests achieving soothing sensitive and/or irritated skin, controlling oil/sebum secretion and desired lightening of the skin apart from serving as an anti-aging formulation.
  • US 3,950,509 discloses a method of reducing body odour by applying to the body an aqueous odour-reducing composition containing a perspiration odor-reducing or retarding amount of a biological protease-ihhibitor .
  • the biological protease-inhibitors proposed for topical application as deodorants include protease-inhibitors such as, for example, kallilrein-trypsin-inhibitors from pancreas, liver, lung, lymph glands, parotid glands, spleen and blood serum and protease inhibitors of vegetable origin, for example the inhibitors from potatoes [A.K.Balis, CA. Ryan, J.
  • the basic object of the present invention to be able to provide products adapted to serve as target oriented inhibitor/functional modifier of proteins such as a system/kit/formulation, useful for such inhibition/functional modification of proteins for cosmetic applications such as an anti-aging aid, anti-microbial applications, malodour inhibition, anti-dandruff applications on scalp as well as for application in food/beverage processing as preservative/storage stability of food stuffs and the like.
  • a system/kit/formulation useful for such inhibition/functional modification of proteins for cosmetic applications such as an anti-aging aid, anti-microbial applications, malodour inhibition, anti-dandruff applications on scalp as well as for application in food/beverage processing as preservative/storage stability of food stuffs and the like.
  • Another object of the present invention is directed to method of cosmetic/personal care applications of the system/kit/formulation of the invention such as for improving/retaining the skin conditions, in anti-microbial applications, anti-dandruff application on scalp and in food processing/preservation and the like.
  • Yet further objective of the invention is directed to be able to provide for an enzyme inhibition system for effecting enzyme inhibition which could serve any one or more of the following i.e. the deterioration in skin condition due to age could be controlled and enable maintaining desired elasticity, youthful suppleness and resilience of the skin even on aging, as anti-microbial agent, anti-dandruff formulation for scalp care, foot care and as a food/beverage preservative.
  • Another object of the present invention is directed to a proteinase inhibition system and its manner of manufacture which would enable controlled proteinase inhibition whereby on one hand the natural body/skin defense characteristics of proteinases could be maintained, and yet the problems of its excessive presence leading to degradation of body/skin conditions could be avoided.
  • Yet further object of the present invention is directed to a proteinase inhibition system and its manner of manufacture which would provide for maintaining desired elasticity of the skin even after it ages thereby controlling to a substantial extent problems of chrono-aging and/or its activated photoageing symptoms/conditions .
  • Yet further object of the present invention is to provide for a proteinase inhibition system and its manner of manufacture which would be safe and compatible with the usual body/skin color/glow/appearance characteristics and/or food product characteristics.
  • Yet further object is directed to a proteinase inhibition system and its manner of manufacture in the form of a kit which would enable controlling problems of uncontrolled proteolytic activity such as skin aging such as skin elasticity, glow, appearance, microbial conditions by way of a simple and cost-effective topical application of the contents of the kit without problems of oily/sticky or otherwise irritating symptoms or visual appearance on use/application.
  • Yet further object of the present invention is directed to provide for a proteinase inhibiting system for cosmetically conditioning personal care and/or conditioning food/beverages free of microbial contamination/problems of storage stability.
  • Yet further object of the present invention is directed to provide for a method of application of the proteinase inhibitor system for cosmetic skin care applications, anti- microbial agent, anti-dandruff applications on scalp and food/beverage processing/storage/packaging and the like.
  • an inhibition system comprising selectively melanin monomers and intermediates as regulation/inhibition of soluble/insoluble proteins.
  • the inhibitor system of the invention comprises a kit comprising: a. a precursor of melanin formation; b. an enzyme or oxidant or a mixture thereof; and c. instructions for application of a) and b) above to regulate/inhibit soluble/insoluble proteins
  • the inhibitor system of the invention comprises a kit for skin care comprising: a. a precursor of melanin formation; b. an enzyme or oxidant or a mixture thereof; and c. instructions for application of a) and b) above to regulate/inhibit protease.
  • the inhibitor system of the invention comprises a kit for use as anti-microbial system comprising: a. a precursor of melanin formation; b. an enzyme or oxidant or a mixture thereof; and c. instructions for application of a) and b) above to regulate growth of microbes.
  • the inhibitor system of the invention comprises a kit for use as food/beverage conditioning/preservation comprising: a. a precursor of melanin formation; b. an enzyme or oxidant or a mixture thereof; and c. instructions for application of a) and b) above to regulate/inhibit soluble/insoluble proteins.
  • the inhibitor system of the invention comprises a kit for controlling body malodour comprising: a. a precursor of melanin formation; b. an enzyme or oxidant or a mixture thereof; and c. instructions of application of a) and b) above to inhibit malodor generation.
  • the inhibitor system of the invention comprises a kit for use as hair or scalp care formulation comprising: a. a precursor of melanin formation; b. an enzyme or oxidant or a mixture thereof; and c. instructions for application of a) and b) above to regulate organisms that are involved in dandruff and in care of the skin of the scalp.
  • the various actives are specifically maintained spatially separated to avoid contamination before use/application.
  • Such spatial separation can be achieved by providing the actives as desired spatially separated forms such as a. separate containers such as bottles containing the respective actives; b. dual-tube compartments for the actives; c. solid supports separated from one another; or d. any other form of providing the actives spatially separated until use.
  • the inhibition system is provided in the form of a kit which comprises: a. precursor of melanin formation which is selected from tyrosine, dihydroxy p enyl alanine (DOPA) , catechol, and other 3, 4-dihydroxy derivatives of phenol or catechol; b. enzyme is selected from tyrosinase, polyphenol oxidase, peroxidases, catechol oxidase and oxidant is selected from hydrogen peroxide, and other peroxides, or metal oxidants selected from FeCl 3 etc or a free radical generating system; c. instructions of application of (a) and (b) above to regulate/inhibit soluble/insoluble proteins/regulate growth of microbes and inhibit generation of malodor.
  • a. precursor of melanin formation which is selected from tyrosine, dihydroxy p enyl alanine (DOPA) , catechol, and other 3, 4-dihydroxy derivatives of phenol or catechol
  • DOPA dihydroxy
  • the selected intermediates of melanin are such that on application to skin they would be capable of inhibiting proteinases, especially elastase and collaginase, increase of which cause ageing of skin by degradation of elastin or collagen.
  • the selective melanin monomers and intermediates of the kit would serve an important role in the regulation/inhibition of such damaging proteinase, and thereby serve as safeguarding the presence of desired elastin and collagen content in skin to maintain its desired elasticity and appearance.
  • the levels/concentration of the various active ingredients range from: i) O.OlmM to 3M precursor of melanin formation; and ii) 0.5 units/ml to 3000 units/ml of an enzyme, or 0.5mM to 3M oxidant, or a free radical generating system, or a mixture thereof.
  • the precursor of melanin is in the range O.OlmM to IM, and more preferably O.OlmM to lOOmM.
  • concentration of the enzyme is preferably 0.5 units/ml to 1000 units/ml, or 0.5mM to IM of the oxidant, or a free radical generating system, or a mixture thereof, and more preferably 0.5 units/ml to 100 units/ml. of an enzyme or 0.5mM to lOOmM oxidant or a free radical generating system or a mixture thereof.
  • the concentration of the various active ingredients range from i) O.OlmM to lO M precursor of melanin formation; and ii) 0.5 units/ml to 20 units/ml of an enzyme, or 0.5mM to lOmM oxidant, or a mixture thereof.
  • the concentration of the various active ingredients range from: i) 0.05 mM to 100 mM precursor of melanin formation; and ii) 1 unit/ml to 250 units/ml enzyme, or ImM to 100 mM oxidant, or a mixture thereof.
  • the levels/concentration of the various active ingredients range from: i) 0.05 mM to 100 mM precursor of melanin formation; and ii) 1 unit/ml to 250 units/ml an enzyme, or ImM to 100 mM oxidants, or a mixture thereof.
  • the levels/cone, of the various active ingredients range from: i) 0.05mM to lOOmM precursor of melanin formation; and ii) 0.5 units to 250 units of an enzyme, or ImM to lOOmM oxidant, or a mixture thereof.
  • the proteinase inhibitor system of the invention is directed to achieve proteinase inhibition such as elastase and collagenase, which cause ageing of the skin by degrading elastin or collagen.
  • the system is thus adapted for regulating/inhibition of these proteinases, and serves as an effective anti-ageing formulation.
  • a method of manufacture of the inhibition system in the form of a kit comprising; Providing in spatially separated form a) a precursor of melanin formation, and b) an enzyme or oxidant or mixture thereof, along with instructions of application of a) and b) to regulate/inhibit soluble/insoluble proteins.
  • the required period of achieving the desired soluble/ insoluble protein inhibition after application of the formulations from the kit of the invention would vary depending upon the conditions treated/attended. For example, for anti-microbial effects the same could be achieved within about 5-10 min, while for anti-ageing the desired time for proteinase inhibition would be about 10 to 30 min. In case of controlling dandruff it would be about 15 to 30 min, while when added to food stuffs and the like it would be about 1 to 5 min.
  • Dihydroxy phenyl alanine (DOPA) and tyrosinase in an acetate buffer (pH 5.0) according to the invention is demonstrated in relation to the following protocol on digestion of Bovine Serum Albumin (BSA) with cathepsin B (CB) .
  • BSA Bovine Serum Albumin
  • CB cathepsin B
  • the two components DOPA and tyrosinase were allowed to react for 2 hours at about 25°C, and only melanin intermediates less than 3000 daltons were used.
  • Bovine Serum Albumin (BSA) was used as the model substrate and cathepsin B as the proteolytic enzyme.
  • BSA Bovine Serum Albumin
  • Cathepsin B were preincubated with and without melanin intermediates, and BSA was used as a substrate and the reaction was allowed to proceed. After the reaction was complete the proteins were precipitated with 10% trichloro acetic acid and washed with diethyl ether.
  • the unreacted protein was analysed on 10% Sodium Dodecyl Sulphate polyacrylamide gel electrophoresis (SDS-PAGE) .
  • SDS-PAGE Sodium Dodecyl Sulphate polyacrylamide gel electrophoresis
  • the results obtained as regards the extent of proteolytic activity achieved is represented in the figure 1.
  • the intensity of the band marked (A) in the figure represents the level of BSA unreacted.
  • Figure 1 shows the following lanes on the gel.
  • a reaction system comprising various components in acetate buffer pH 5.0 as described in Table 1 was used to assay the anti-microbial activity at 30°C.
  • a gram positive bacteria for e.g. Staphylococcus aureus (S. aure ⁇ s) and a gram negative bacteria for e.g. Escherichia coli (E. coli) cultures were used for testing the inhibitory activity of the melanin monomers on their growth in these systems.
  • the data presented in table 1 show that in presence of the melanin monomers that get generated in the test system when the precursor such as catechol and an oxidant such as ferric chloride or an enzyme such as tyrosinase are present have a significant antimicrobial activity. In the absence of any one of the ingredients namely, the precursor or the oxidant or the enzyme, no inhibition of growth is observed. At the end of 10 minutes, significant kill of the bacteria was observed showing the antimicrobial activity of the melanin monomers generated according to the invention.
  • the cells of the bacteria were separated from the medium by centrifugation and resuspended in 1ml physiological saline, and sonicated for 10 minutes. This suspension was incubated at 37°C ' for 1 hour with and without the melanin monomers.
  • the melanin monomers were formed by reacting DOPA with tyrosinase.
  • the level of the malodour component (butyric acid) generated was scored on a 1 to 5 scale, where 1 represents low value and 5 represents high value. It was seen that in the presence of the melanin monomers, the malodour component was (1) and was significantly lower than the control (4) .

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Abstract

L'invention concerne un ensemble d'inhibition composé (a) d'un précurseur de la formation de mélanine ; (b) d'une enzyme, d'un oxydant ou d'un mélange de ceux-ci ; et (c), d'instructions destinées à l'application de (a) et de (b) pour la régulation/inhibition de protéines solubles/insolubles, la régulation/inhibition de protéase destinée au soin de la peau, la régulation de la croissance microbienne destinée à la constitution d'un système antimicrobien, la régulation/inhibition de protéines solubles/insolubles destinée au conditionnement ou à la conservation d'aliments ou de boissons, l'inhibition de la production de mauvaises odeurs, et la régulation d'organismes impliqués dans la formation de pellicules et le soin du cuir chevelu.
PCT/EP2003/014513 2002-12-30 2003-12-17 Composition cosmetique contenant un precurseur de melanine WO2004058210A1 (fr)

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AU2003290087A AU2003290087A1 (en) 2002-12-30 2003-12-17 Cosmetic composition containing a melanin precursor

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IN1164/MUM/02 2002-12-30
IN1164MU2002 2002-12-30

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WO (1) WO2004058210A1 (fr)

Cited By (1)

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EP3139768A4 (fr) * 2014-05-06 2017-12-27 Arturo Solis Herrera Procédé et système électrochimique pour la conservation de denrées périssables

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JPS54110337A (en) * 1978-02-14 1979-08-29 Nonogawa Shoji Yk Hair dyeing agent
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WO1998051269A1 (fr) * 1997-05-16 1998-11-19 Bristol-Myers Squibb Company Teinture des cheveux a l'aide de precurseurs de la melanine en presence d'iodate et de peroxyde
WO2000069267A1 (fr) * 1999-05-14 2000-11-23 University Of Otago Compositions resistantes aux microbes

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US3993436A (en) * 1973-12-01 1976-11-23 Shiseido Co., Ltd. Dyeing live hair with melanin precursors
JPS54110337A (en) * 1978-02-14 1979-08-29 Nonogawa Shoji Yk Hair dyeing agent
US4473550A (en) * 1981-01-16 1984-09-25 Rosenbaum Robert S Bactericidal compositions and methods
US4609544A (en) * 1983-07-05 1986-09-02 Repligen Corporation Process for tanning the skin
WO1998051269A1 (fr) * 1997-05-16 1998-11-19 Bristol-Myers Squibb Company Teinture des cheveux a l'aide de precurseurs de la melanine en presence d'iodate et de peroxyde
WO2000069267A1 (fr) * 1999-05-14 2000-11-23 University Of Otago Compositions resistantes aux microbes

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3139768A4 (fr) * 2014-05-06 2017-12-27 Arturo Solis Herrera Procédé et système électrochimique pour la conservation de denrées périssables
US11432572B2 (en) 2014-05-06 2022-09-06 Arturo Solis Herrera Electrochemical process and system for the preservation of perishable food

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GB0306084D0 (en) 2003-04-23

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