WO2004056484A1 - Systeme microstructure pour le traitement d'un fluide - Google Patents

Systeme microstructure pour le traitement d'un fluide Download PDF

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Publication number
WO2004056484A1
WO2004056484A1 PCT/DE2003/004206 DE0304206W WO2004056484A1 WO 2004056484 A1 WO2004056484 A1 WO 2004056484A1 DE 0304206 W DE0304206 W DE 0304206W WO 2004056484 A1 WO2004056484 A1 WO 2004056484A1
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WO
WIPO (PCT)
Prior art keywords
reservoir
fluid
channel
electrode
microstructured
Prior art date
Application number
PCT/DE2003/004206
Other languages
German (de)
English (en)
Inventor
Markus Pfister
Steve Reichel
Original Assignee
Siemens Aktiengesellschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Siemens Aktiengesellschaft filed Critical Siemens Aktiengesellschaft
Publication of WO2004056484A1 publication Critical patent/WO2004056484A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus

Definitions

  • the invention relates to a microstructured arrangement for treating a fluid according to the preamble of claim 1.
  • Treatment means the implementation of reactions, but in particular the analysis, for example by capillary electrophoresis.
  • a microchip laboratory system has several reservoirs, which are formed in a substrate together with a channel system in a micromechanical way. Some of the reservoirs contain fluids to be processed which, after being processed in the duct system, are collected as waste products in other reservoirs. The fluids are conveyed from one reservoir to another electrokinetically by applying an electrical voltage between the respective reservoirs, for which purpose electrodes are arranged in them which can be switched to a voltage source.
  • the invention is based, to improve the microstructured arrangement of the type mentioned for a continuous fluid supply the task.
  • the fluid flows continuously through the reservoir, which for this purpose, for example, continuously from an industrial or other Process is removed and pumped through the reservoir.
  • the residence time of the fluid from the time of its removal from the process to the time at which a part of it is electrokinetically branched off from the reservoir for further processing and conveyed into the channel is therefore given a correspondingly high volume flow of the fluid flowing through the reservoir very short and independent of the time or the times of the turn. Since the branching of part of the fluid from the reservoir into the channel, viewed in the direction of flow, takes place in front of the electrode arranged in the reservoir, glass bubbles formed on the electrode cannot get into the channel and block it or a subsequent reaction or analysis system.
  • the channel When viewed from the electrode, the channel preferably branches off from the reservoir at an angle ⁇ 90 °, so that the fluid which flows through the reservoir is not pressed into the channel.
  • the reservoir is preferably also designed as a channel through which the fluid flows in a pressure-driven manner.
  • the electrode is preferably arranged such that it projects into the flow path of the fluid through the reservoir and the fluid flows around it. This makes it possible to design the electrode as a robust wire electrode that is less rapidly degraded by electrolysis than, for example, a thin film electrode. So that the flow of the fluid through the electrode is not disturbed, the interior of the reservoir can be widened in the area of the protruding electrode, so that the flow cross-section of the reservoir remains at least approximately constant.
  • the channel and the reservoir can be designed in different ways as microfluidic structures, wherein they are preferably in the form of trench-shaped depressions on one side of a plate and are covered by a cover plate lying thereon.
  • FIG. 1 shows an embodiment of the microstructured arrangement according to the invention for analyzing a fluid
  • FIGS. 2 and 3 show an example of a reservoir through which the fluid flows and with a branch branching from it in two different sectional views.
  • FIG. 1 shows a microstructured arrangement consisting of a carrier, here a plate 1, on which reservoirs 2, 3, 4 and 5 for fluids and a channel system 6 are formed micromechanically.
  • the reservoirs 2 to 5 and the channel system 6 are designed here as trench-shaped depressions on one side of the plate 1 and covered by a cover plate (not shown here) lying on top.
  • the channel system 6 consists of four channels 7, 8, 9 and 10, each branching off from one of the four reservoirs 2 to 5 and connected to one another at an intersection 11.
  • electrodes 12, 13, 14 and 15 are arranged, which are connected to a four-pole voltage source 16, via which different high voltages can be set between the electrodes 12 to 15.
  • fluids from predeterminable reservoirs e.g. B. 2 and 3, via the channel system 6 in other reservoirs, for. B. 4 and 5, electro-kinetically promoted.
  • a buffer fluid is conveyed from the reservoir 3 via the channels 8 and 9 into the reservoir 4 and at the same time a sample fluid is conveyed from the reservoir 2 via the channels 7 and 10 into the reservoir 5.
  • the sample fluid at the intersection 11 is introduced into the channel 9 for a predetermined time and thus in a predetermined amount, before it is subsequently redirected into the channel 10.
  • the sample fluid introduced into the channel 9 and then conveyed through the channel 9 through the subsequent buffer fluid is electrophoretically separated into its components in the channel 9, which are detected by means of a detector device 17; an evaluation device 18 arranged downstream of the detector device 17 determines the detected components of the sample fluid qualitatively and quantitatively.
  • a sample fluid can be conveyed from the reservoir 3 via the channels 8 and 10 and a buffer fluid from the reservoir 2 via the channels 7 and 10 into the reservoir 5.
  • the buffer fluid at the crossing point 11 is diverted into the channel 9 and thereby injects the sample fluid located in the area of the crossing point 11 into the channel 9.
  • the amount of the sample fluid injected into the channel 9 corresponds to the volume of the crossing point 11 and can be increased by the fact that the channel 10 opens into the channel 9 offset from the channel 8 in the direction of the detector device 17.
  • the reservoirs 2 to 5 are designed in the form of further channels with fluid connections 19, 20, 21, 22, 23 and 24.
  • the reservoirs 2 used to feed the fluid into the channel system 6 and 3 each have two fluid connections 19 and 20 or 21 and 22, the channels 7 and 8 each branching off from the reservoirs 2 and 3 in the region between the two fluid connections 19 and 20 or 21 and 22.
  • the sample fluid 25 is taken continuously from an industrial process, here a pipeline 26, and after preparation, for example filtering and buffering, in a sample preparation device 27 by a pump or the like, pressure-driven via the fluid.
  • the sample fluid 25 flows through the reservoir 2 and is disposed of via the fluid connection 20.
  • FIGS. 2 and 3 each show, in different sectional representations, a section of the reservoir 2 in the area between the two fluid connections 19 and 20.
  • the reservoir 2 is designed in the form of a further channel 28 in the plate 1 and covered by a cover plate 29.
  • the electrode 12 is wire-shaped at least in its end region and penetrates the plate 1 coming from the side facing away from the channels 7, 28. Furthermore, the electrode 12 extends across the reservoir 2 to the cover plate 29, so that the sample fluid 25 flows around it on both sides.
  • the reservoir 2 is widened in the region of the electrode 12, so that its flow cross section narrows only slightly or not at all at this point.
  • the branch of the channel 7 from the reservoir 2 is arranged in front of the electrode 12 in relation to the direction of flow of the sample fluid 25, so that gas bubbles 30 formed on the electrode 12 by electrolysis are transported away from the branch by the flowing sample fluid 25 and are carried out and thus do not get into channel 7.
  • the channel-shaped reservoir 2 and the channel 7 branching therefrom each have a rectangular gen, here square flow cross-section, wherein the flow cross-section of the reservoir 2 with 0.3 x 0.3 mm, for example, is larger than that of the channel 7 with, for example, 0.1 x 0.1 mm.
  • the cross section of the channel 7 is sufficiently small to enable the sample fluid 25 to be conveyed electrokinetically.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Electrostatic Separation (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

Système microstructuré pour le traitement d'un fluide, possédant un réservoir qui contient le fluide et dans lequel est placée une électrode. Un conduit conduisant à une autre électrode part du réservoir. L'application d'une tension électrique sur les électrodes provoque un refoulement électrocinétique d'une partie du fluide, du réservoir dans le conduit. Selon la présente invention, pour que ledit système produise une alimentation continue en fluide, le fluide (25) traverse le réservoir (2) en continu et l'embranchement du conduit (7) à partir du réservoir (2) est situé dans le réservoir (2) avant l'électrode (12), par rapport à la direction d'écoulement du fluide (25).
PCT/DE2003/004206 2002-12-20 2003-12-19 Systeme microstructure pour le traitement d'un fluide WO2004056484A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10260310.3 2002-12-20
DE2002160310 DE10260310B3 (de) 2002-12-20 2002-12-20 Mikrostrukturierte Anordnung zur Behandlung eines Fluids

Publications (1)

Publication Number Publication Date
WO2004056484A1 true WO2004056484A1 (fr) 2004-07-08

Family

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Family Applications (1)

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PCT/DE2003/004206 WO2004056484A1 (fr) 2002-12-20 2003-12-19 Systeme microstructure pour le traitement d'un fluide

Country Status (2)

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DE (1) DE10260310B3 (fr)
WO (1) WO2004056484A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004062923A1 (de) * 2004-12-28 2006-07-06 Hirschmann Laborgeräte GmbH & Co. KG Vorrichtung zur Förderung von Fluiden, Verfahren zur Herstellung derselben und Pipette mit einer solchen Vorrichtung
CN111700632A (zh) * 2019-03-18 2020-09-25 西门子医疗有限公司 用于局部减弱x射线辐射的过滤系统、x射线仪器和用于局部改变x射线辐射的强度的方法

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL1038266C2 (nl) * 2010-09-27 2012-03-28 Capilix B V Meetsysteem en werkwijze voor het autonoom meten van een ion-concentratie met microchip capillaire elektroforese.
CN102764676B (zh) * 2012-07-23 2014-08-06 西安交通大学 非接触式光驱动-双极电极的微流控芯片

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5846396A (en) * 1994-11-10 1998-12-08 Sarnoff Corporation Liquid distribution system
EP1162455A1 (fr) * 1994-08-01 2001-12-12 Lockheed Martin Energy Systems, Inc. Méthode et appareil pour la performance des manipulations microfluidics pour l'analyse et synthèse chimique
WO2002064253A2 (fr) * 2001-02-09 2002-08-22 Microchem Solutions Procédé et dispositif d'injection d'échantillons dans des dispositifs fabriqués par micro-usinage
EP1241472A2 (fr) * 1996-07-03 2002-09-18 Caliper Technologies Corporation Contrôle variable par des forces électriques de forces électroosmotiques et/ou électrophorétiques dans une enceinte contenant un fluide

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5699157A (en) * 1996-07-16 1997-12-16 Caliper Technologies Corp. Fourier detection of species migrating in a microchannel

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1162455A1 (fr) * 1994-08-01 2001-12-12 Lockheed Martin Energy Systems, Inc. Méthode et appareil pour la performance des manipulations microfluidics pour l'analyse et synthèse chimique
US5846396A (en) * 1994-11-10 1998-12-08 Sarnoff Corporation Liquid distribution system
EP1241472A2 (fr) * 1996-07-03 2002-09-18 Caliper Technologies Corporation Contrôle variable par des forces électriques de forces électroosmotiques et/ou électrophorétiques dans une enceinte contenant un fluide
WO2002064253A2 (fr) * 2001-02-09 2002-08-22 Microchem Solutions Procédé et dispositif d'injection d'échantillons dans des dispositifs fabriqués par micro-usinage

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004062923A1 (de) * 2004-12-28 2006-07-06 Hirschmann Laborgeräte GmbH & Co. KG Vorrichtung zur Förderung von Fluiden, Verfahren zur Herstellung derselben und Pipette mit einer solchen Vorrichtung
CN111700632A (zh) * 2019-03-18 2020-09-25 西门子医疗有限公司 用于局部减弱x射线辐射的过滤系统、x射线仪器和用于局部改变x射线辐射的强度的方法
CN111700632B (zh) * 2019-03-18 2023-08-29 西门子医疗有限公司 用于局部减弱x射线辐射的过滤系统、x射线仪器和用于局部改变x射线辐射的强度的方法

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Publication number Publication date
DE10260310B3 (de) 2004-05-06

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